CN105766368A - Method for cultivating lepista sordida (Schum.: Fr.) Sing.with miscanthus sacchariflorus and culture medium of method - Google Patents
Method for cultivating lepista sordida (Schum.: Fr.) Sing.with miscanthus sacchariflorus and culture medium of method Download PDFInfo
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- CN105766368A CN105766368A CN201610112434.XA CN201610112434A CN105766368A CN 105766368 A CN105766368 A CN 105766368A CN 201610112434 A CN201610112434 A CN 201610112434A CN 105766368 A CN105766368 A CN 105766368A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
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- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention discloses a method for cultivating lepista sordida (Schum.: Fr.) Sing.with miscanthus sacchariflorus and a culture medium of the method.The culture medium is composed of, by weight, 75-80% of the miscanthus sacchariflorus, 17-20% of wheat bran, 1-2% of urea and 2-3% of gypsum, the water content of the culture medium is controlled to be 65-70%, and pH is 7.5-8.5; the cultivation method includes the steps that a small segment of the miscanthus sacchariflorus is placed into lime water for soaking, and after the miscanthus sacchariflorus is dried, the wheat bran, the urea and the gypsum are added, mixed and stirred; then heap building is conducted, air holes are formed in a punching mode, membrane covering is conducted, heap turning is conducted when the material temperature is raised to 70 DEG C or above, heap turning is conducted once every day, and water is added for preserving moisture when the water content is lower than 65%; fermentation is conducted for 13-17 days; bed building is conducted, inoculation is conducted when the material temperature reaches 20-30 DEG C, and the weight ratio of the material to seeds is 5: 1, compression is conducted, and the heap is covered with turfy soil which is 1.8-2.2 cm in thickness; culture is conducted for 30 days at the temperature of 20-25 DEG C.According to the method, planting raw materials are sufficient, low in price and good in quality.
Description
Technical field:
The present invention relates to a kind of method using reed grass cultivation Lepista sordida and culture medium thereof, belong to biological technical field.
Background technology:
Lepista sordida belongs to mycota Fungi, Basidiomycota Basidiomycota, agaric guiding principle Agaricomycetes, agaric
Mesh Agaricales, Tricholomataceae Tricholomataceae, the macro fungi of Lepista lentinus Lepista.It is fresh and tender sweet, fragrance
Strong, mouthfeel is fabulous, have the good reputation of " a Shiqi taste hears its perfume for ten ".In Europe, the pouplarity energy of Lepista sordida
Enough equally celebrated for their achievements with matsutake and bolete, it is considered first-class food materials in France, there is the highest edibility.
Additionally, Lepista sordida not only delicious flavour, and vitamin containing many needed by human and amino acid and rich
Rich protein, such as threonine, alanine, glutamine, serine, glutamic acid, valine, leucine, asparagine acid,
Asparatate and glycine[8].In addition the fructification of Lepista sordida possibly together with mannitol, arabite, glucose, glycerine,
Fructose and trehalose, wherein the content of trehalose is the highest.
In terms of medical value, " the book on Chinese herbal medicine art warp " embroidered according to " daily draft " and the Qing Dynasty Huang official in the Yuan Dynasty is recorded, and eats for a long time
Vascular sclerosis can be effectively prevented with Lepista sordida, cirrhosis, diabetes, kidney trouble, gall stone etc. can be treated.Up till now
Till, the research about the medical value of Lepista sordida abroad mainly still concentrates on its antioxidation activity and antibacterial activity two
In aspect.The fructification of Lepista sordida is possibly together with abundant Cobastab1, it is possible to the regulation normal glycometabolism of organism, promote god
Through conduction, prevention tinea pedis there is good effect.The hot water extract of Lepista sordida fructification has significant active anticancer, its
Pharmacological testing proves that Lepista sordida fruiting body extract is respectively 100% He to the inhibiting rate of small white mouse Emhorn knurl and euphorbia egg decoctum
90%.And the juice that fructification decocts can help to treat abscess, sand holes and wound.
In recent years, Lepista sordida is gradually recognized understanding by common people, is continuously increased its market demand, thus causes open country
Production-goods source is reduced year by year, is difficult to meet the demand in market again, Lepista sordida carries out artificial cultivation technique research imperative.
The ability that Lepista sordida drafts a document saprophytic bacteria, decomposition of cellulose and lignin is weak.At present in cultivation culture medium wood chip,
Straw, wheat straw, bagasse, cotton seed hulls, waste cotton and corncob are primary carbon source, with livestock and poultry muck such as chicken manure, horsehit and cow dungs or
Adding appropriate wheat bran, corn flour, soy meal and various grouts is nitrogen source, and there is lack of raw materials and price high, poor quality, and along with edible
The continuous expansion of bacterium plant husbandry, cotton seed hulls price is more and more expensive, substantially increases Lepista sordida planting cost.
Reed grass [Triarrhena sacchariflora (Maxim.) Nakai] is grass family, and reed belongs to perennial grass
This plant.Crawling root-like stock, stalk is upright, high up to 1.5 meters, diameter about 5 millimeters, the raw pubescence of joint.Leaf sheath is without hair, and the tip of a leaf is short, tool
Cilium;Blade is flat, wide linear, and edge sawtooth shape is coarse, and base portion is often shrunk to handle, sturdy.Panicle dredges generated umbrella room shape,
Main shaft, without hair, gives birth to pubescence between armpit, small ear handle top is slightly expanded, small ear wire lanceolar, band brown after maturation, and basal disc tool is a length of little
The thread pubescence of fringe 2 times;Top film quality length is tapering, and edge and back have long pubescence;Caryopsis Long Circle, yields positive results the 8-10 month.
Widely distributed in China, Heilungkiang, Jilin, Liaoning, Hebei, Shanxi, Henan, Shandong, Gansu and Shaanxi etc. save, and are born in hillside grass
Ground and hillock, Plain, riparian wetland.
There is presently no the primary raw material using reed grass as Lepista sordida, substitute the paint face such as cotton seed hulls, wood chip, wheat straw fragrant
The Main Cultivation raw material of mushroom plantation, provides a kind of not only economic flow rate but also high novel cultivation technique for Lepista sordida cultivation, simultaneously
Reduce the relevant report of the planting cost of Lepista sordida.
Summary of the invention:
It is an object of the invention to overcome the deficiency of above-mentioned prior art to provide a kind of plantation raw material sufficient, quality low with price
Good, the mycelial growth rate of Lepista sordida can be improved, the method using reed grass cultivation Lepista sordida of high yield and training thereof simultaneously
Support base.
The purpose of the present invention can be reached by following measure: a kind of for using the cultivation of reed grass cultivation Lepista sordida
Base, it is characterised in that its recipe ingredient is by weight percentage: reed grass 75-80%, wheat bran 17-20%, urea 1-2%, gypsum 2-
3%, the water content control of culture medium is 7.5-8.5 at 65-70%, pH.
In order to realize the purpose of the present invention further, its recipe ingredient is by weight percentage: reed grass 79%, wheat bran 17%,
Urea 1%, gypsum 2%, the water content control of culture medium are 7.5-8.5 at 65-70%, pH.
A kind of method using reed grass cultivation Lepista sordida, it is characterised in that it comprises the steps:
(1) reed grass pre-treatment:
Reed grass is cut into the segment of a length of 3-4 centimetre, puts in limewash and soak, soak 10-12 hour, take out to drench and do
Moisture, the water content control of reed grass is at 70-75%；Wherein the content of lime is the 5% of reed grass weight, and limewash is completely soaked reed
Grass.
(2) prepared by culture medium:
By weight percentage by above-mentioned soaked reed grass 75-80%, add wheat bran 17-20%, urea 1-2%, gypsum 2-3%, enter
Row is sufficiently mixed stirring, and finally making water content control is 7.5-8.5 at 65-70%, pH；
(3) cultivate capital construction heap to ferment:
By be stirred culture medium carry out building heap fermentation, heap is high 1.5 meters, wide 1.5 meters, every 70 centimetres, pricks one a diameter of 5
Centimetre passage, the through bottom of passage, and overlay film, treat that material temperature rises at least 70 DEG C and carries out turning under turning, top, the inside
Turn up, turning every day once, adds water moisturizing when water content is less than 65%；Ferment and within 13-17 days, complete fermentation, make compost；
(4) bed is built, inoculation:
The compost fermented carries out building bed, bedside 1.4, thick 15 centimetres of material, treats material temperature to 20 DEG C-30 DEG C, accesses through purifying
Lepista sordida bacterial classification, the weight ratio of the compost fermented and Lepista sordida bacterial classification is 5:1, and then compacting allows Lepista sordida bacterium
Planting and be fully contacted with compost, then surface covers the turfy soil of 1.8-2.2 centimetre；
(5) cultivate:
Connecting after planting, control indoor temperature to cultivate at 20-25 DEG C, between bacteria developing period, as found, overburden layer becomes dry, and waters in time
Water moisturizing, cultivates 30 days, both can be with fruiting.
The present invention can produce following good effect compared with the prior art: Lepista sordida is a kind of rare, high-quality wild mushroom
Kind, under wild state, quantity is few, yields poorly but its color and luster grace, and smell giving off a strong fragrance, taste is fresh and tender, nutritive value and medicinal valency
It is worth high and expensive.At present, the primary raw material of Lepista sordida cultivation is cotton seed hulls, wheat straw and wood chip etc., cost of material
Expensive, planting cost is high, and the problem such as yield poorly, and reed grass that compost raw material of the present invention is used, it is wild widely distributed, yield
Greatly, plant height, harvesting of holding, is preferable Lepista sordida culturing raw material, and its content of lignin is low, cellulose and hemicellulose level
Height, dense, sturdy compared with other culture medium mycelium length, in lavender, viability is vigorous, can shorten Lepista sordida mycelium nutrition
The time of growth, promote it to be changed into reproductive growth from nourishing and growing at short notice, shorten the cultivation period of Lepista sordida,
Accomplish scale production for Lepista sordida and the development of medicinal career all has very important significance.The present invention uses reed grass to make
For the primary raw material of Lepista sordida, substitute the Main Cultivation raw material of the Lepista sordida plantations such as cotton seed hulls, wood chip, wheat straw, for paint face
Fragrant mushroom cultivation develops a kind of not only economic flow rate but also high novel cultivation technique, reduces the raw material caused due to raw material anxiety simultaneously
The vicious circle that price is the most expensive, reduces the planting cost of Lepista sordida, shortens the crop cycle of Lepista sordida, improves and produces
Amount.
Detailed description of the invention:
Below the detailed description of the invention of the present invention is elaborated:
Embodiment 1:
(1) reed grass pre-treatment:
Reed grass is cut into the segment of a length of 3 centimetres, puts in limewash and soak, soak 10 hours, take out pouring solid carbon dioxide and divide,
The water content control of reed grass is 70%；Wherein the content of lime is the 5% of reed grass weight, and limewash is completely soaked reed grass.
(2) prepared by culture medium:
By weight percentage by above-mentioned soaked reed grass 80%, add wheat bran 17%, urea 1%, gypsum 2%, be sufficiently mixed
Stirring, finally makes water content control 70%, and pH is 7.5.
(3) cultivate capital construction heap to ferment:
By be stirred culture medium carry out building heap fermentation, heap is high 1.5 meters, and wide 1.5 meters, length does not limits, and every 70 centimetres, pricks one
The passage of individual a diameter of 5 centimetres, the through bottom of passage, in overlay film insulation autumn and winter, summer, the preventing and treating of overcast and rainy overlay film drenched with rain,
Treat that material temperature rises at least 70 DEG C and carries out turning under turning, top, turning up of the inside, turning every day once, when water content is less than 65%
Time suitably add water moisturizing；Ferment and within 13 days, complete fermentation, make compost；The compost fermented has material perfume, without going mouldy, quality
Softness, tack-free.
(4) bed is built, inoculation:
The compost fermented carries out building bed, bedside 1.4, thick 15 centimetres of material, treats material temperature to 20 DEG C, accesses purified paint face
Fragrant mushroom bacterial classification, the compost fermented is 5:1 with the weight ratio of Lepista sordida bacterial classification, and then compacting allows Lepista sordida bacterial classification and training
Nutriment is fully contacted, and then surface covers the turfy soil of 1.8 centimetres.
(5) cultivate:
Connecting after planting, control indoor temperature to cultivate at 20 DEG C, between bacteria developing period, as found, overburden layer becomes dry, and water guarantor in time
Wet, cultivate 30 days, both can be with fruiting.
Embodiment 2:
(1) reed grass pre-treatment:
Reed grass is cut into the segment of a length of 4 centimetres, puts in limewash and soak, soak 12 hours, take out pouring solid carbon dioxide and divide,
The water content control of reed grass is 75%；Wherein the content of lime is the 5% of reed grass weight, and limewash is completely soaked reed grass.
(2) prepared by culture medium:
By weight percentage by above-mentioned soaked reed grass 75%, add wheat bran 20%, urea 2%, gypsum 3%, be sufficiently mixed
Stirring, finally makes water content control 65%, and pH is 8.5.
(3) cultivate capital construction heap to ferment:
By be stirred culture medium carry out building heap fermentation, heap is high 1.5 meters, and wide 1.5 meters, length does not limits, and every 70 centimetres, pricks one
The passage of individual a diameter of 5 centimetres, the through bottom of passage, in overlay film insulation autumn and winter, summer, the preventing and treating of overcast and rainy overlay film drenched with rain,
Treat that material temperature rises at least 70 DEG C and carries out turning under turning, top, turning up of the inside, turning every day once, when water content is less than 65%
Time suitably add water moisturizing；Ferment and within 17 days, complete fermentation, make compost；The compost fermented has material perfume, without going mouldy, quality
Softness, tack-free.
(4) bed is built, inoculation:
The compost fermented carries out building bed, bedside 1.4, thick 15 centimetres of material, treats material temperature to 30 DEG C, accesses purified paint face
Fragrant mushroom bacterial classification, the compost fermented is 5:1 with the weight ratio of Lepista sordida bacterial classification, and then compacting allows Lepista sordida bacterial classification and training
Nutriment is fully contacted, and then surface covers the turfy soil of 2.2 centimetres.
(5) cultivate:
Connecting after planting, control indoor temperature to cultivate at 25 DEG C, between bacteria developing period, as found, overburden layer becomes dry, and water guarantor in time
Wet, cultivate 30 days, both can be with fruiting.
Embodiment 3:
(1) reed grass pre-treatment:
Reed grass is cut into the segment of a length of 3.5 centimetres, puts in limewash and soak, soak 11 hours, take out and drench solid carbon dioxide
Point, the water content control of reed grass is 72%；Wherein the content of lime is the 5% of reed grass weight, and limewash is completely soaked reed grass.
(2) prepared by culture medium:
By weight percentage by above-mentioned soaked reed grass 78%, add wheat bran 18%, urea 1.5%, gypsum 2.5%, carry out fully
Mix and blend, finally makes water content control 68%, and pH is 8.
(3) cultivate capital construction heap to ferment:
By be stirred culture medium carry out building heap fermentation, heap is high 1.5 meters, and wide 1.5 meters, length does not limits, and every 70 centimetres, pricks one
The passage of individual a diameter of 5 centimetres, the through bottom of passage, in overlay film insulation autumn and winter, summer, the preventing and treating of overcast and rainy overlay film drenched with rain,
Treat that material temperature rises at least 70 DEG C and carries out turning under turning, top, turning up of the inside, turning every day once, when water content is less than 65%
Time suitably add water moisturizing；Ferment and within 15 days 5, complete fermentation, make compost；The compost fermented has material perfume, without going mouldy, matter
Ground is soft, tack-free.
(4) bed is built, inoculation:
The compost fermented carries out building bed, bedside 1.4, thick 15 centimetres of material, treats material temperature to 25 DEG C, accesses purified paint face
Fragrant mushroom bacterial classification, the compost fermented is 5:1 with the weight ratio of Lepista sordida bacterial classification, and then compacting allows Lepista sordida bacterial classification and training
Nutriment is fully contacted, and then surface covers the turfy soil of 2 centimetres.
(5) cultivate:
Connecting after planting, control indoor temperature to cultivate at 23 DEG C, between bacteria developing period, as found, overburden layer becomes dry, and water guarantor in time
Wet, cultivate 30 days, both can be with fruiting.
Above embodiment is only to be described the preferred embodiment of the present invention, not enters the scope of the present invention
Row limits, on the premise of designing spirit without departing from the present invention, and this area ordinary skill technical staff technical side to the present invention
Various deformation that case is made and improvement, all should fall in the protection domain that claims of the present invention determines.
Claims (3)
1. the culture medium being used for using reed grass cultivation Lepista sordida, it is characterised in that its recipe ingredient is by weight percentage
For: reed grass 75-80%, wheat bran 17-20%, urea 1-2%, gypsum 2-3%, the water content control of culture medium at 65-70%, pH is
7.5-8.5。
The most according to claim 1 a kind of for using the culture medium of reed grass cultivation Lepista sordida, it is characterised in that it is joined
Fang Zufen is by weight percentage: reed grass 79%, wheat bran 17%, urea 1%, gypsum 2%, culture medium water content control at 65-
70%, pH are 7.5-8.5.
3. the method using reed grass cultivation Lepista sordida, it is characterised in that it comprises the steps:
(1) reed grass pre-treatment:
Reed grass is cut into the segment of a length of 3-4 centimetre, puts in limewash and soak, soak 10-12 hour, take out to drench and do
Moisture, the water content control of reed grass is at 70-75%；Wherein the content of lime is the 5% of reed grass weight, and limewash is completely soaked reed
Grass；
(2) prepared by culture medium:
By weight percentage by above-mentioned soaked reed grass 75-80%, add wheat bran 17-20%, urea 1-2%, gypsum 2-3%, enter
Row is sufficiently mixed stirring, and finally making water content control is 7.5-8.5 at 65-70%, pH；
(3) cultivate capital construction heap to ferment:
By be stirred culture medium carry out building heap fermentation, heap is high 1.5 meters, wide 1.5 meters, every 70 centimetres, pricks one a diameter of 5
Centimetre passage, the through bottom of passage, and overlay film, treat that material temperature rises at least 70 DEG C and carries out turning under turning, top, the inside
Turn up, turning every day once, adds water moisturizing when water content is less than 65%；Ferment and within 13-17 days, complete fermentation, make compost；
(4) bed is built, inoculation:
The compost fermented carries out building bed, bedside 1.4, thick 15 centimetres of material, treats material temperature to 20 DEG C-30 DEG C, accesses through purifying
Lepista sordida bacterial classification, the weight ratio of the compost fermented and Lepista sordida bacterial classification is 5:1, and then compacting allows Lepista sordida bacterium
Planting and be fully contacted with compost, then surface covers the turfy soil of 1.8-2.2 centimetre；
(5) cultivate:
Connecting after planting, control indoor temperature to cultivate at 20-25 DEG C, between bacteria developing period, as found, overburden layer becomes dry, and waters in time
Water moisturizing, cultivates 30 days, both can be with fruiting.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109673386A (en) * | 2018-11-21 | 2019-04-26 | 达州市农业科学研究院（达州市苎麻科学研究所达州市薯类作物研究所） | A kind of Lepista sordida bacterium culture medium and preparation method and cultural method |
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Cited By (1)
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| CN109673386A (en) * | 2018-11-21 | 2019-04-26 | 达州市农业科学研究院（达州市苎麻科学研究所达州市薯类作物研究所） | A kind of Lepista sordida bacterium culture medium and preparation method and cultural method |
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