CN105766129A - Method for releasing dormancy of oil-used peony seeds - Google Patents
Method for releasing dormancy of oil-used peony seeds Download PDFInfo
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- CN105766129A CN105766129A CN201610141018.2A CN201610141018A CN105766129A CN 105766129 A CN105766129 A CN 105766129A CN 201610141018 A CN201610141018 A CN 201610141018A CN 105766129 A CN105766129 A CN 105766129A
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- paeonia suffruticosa
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention discloses a method for releasing dormancy of oil-used peony seeds. The method comprises the following steps: a quick post-maturation stage of seed embryos of the oil-used peony seeds: harvesting peony pods, spreading the peony pods in shade indoors, and overturning up and down once very day; after the pods crack naturally, screening and collecting shed seeds; performing stratification treatment after full seeds which submerge in water are dried in shade indoors for 1 to 2 days; adding fresh river sand which contains 50 to 60 mass percent of water in the screened seeds, and spreading a mixture indoors for drying; keeping internal temperature and humidity of a mixture of the seeds and the river sand, and treating for 30 days to obtain the mixture of the seeds with the seed embryos which are completely matured and the river sand; a hypocotyledonary axis dormancy releasing stage of the oil-used peony seeds: controlling the indoor temperature of the mixture of the seeds and the river sand to be between 15 and 20 DEG C, keeping the humidity of the river sand, and treating for 30 days to obtain a mixture of the seeds of which the root length is greater than 4 centimetres and the river sand; an epicotyl dormancy releasing stage of the oil-used peony seeds: controlling the indoor temperature of the mixture of the seeds of which the root length is greater than 4 centimetres and the river sand to be below 10 DEG C; spraying a plant growth conditioning agent; keeping the river sand at a certain humidity; treating for 30 days; completing a dormancy releasing process of the oil-used peony seeds.
Description
Technical field
The invention belongs to seed dormancy and release technical field, a kind of method relating to oil seed of Paeonia suffruticosa termination of diapause.
Background technology
Paeonia suffruticosa, is under the jurisdiction of Paeoniaceae (Paeoniaceae) Paeonia (Paeonia) Section Moutan (Sect.MoutanDC.), is traditional famous flower of China, have the good reputation of " king in spending ", " aromatic ".China is the centre of origin, the world and the distribution center of Paeonia suffruticosa, and whole 9 kinds of this group all originate in China.As medicinal plants, Paeonia suffruticosa is at China's cultivation history of existing 2000；As ornamental plant, Paeonia suffruticosa is at China's cultivation history of existing 1600.
In recent years, it has been found that in peony seed oil, the content of unsaturated fatty acid is more than 90%, meanwhile, a linolenic acid content is up to 40%.2011, peony seed oil was approved as " new resource food " by China's Ministry of Public Health.The oil of seed of Flos Moutan is worth the concern being just subject to more and more people, and oil is also about to by Paeonia suffruticosa industry rise.After this industry is risen, oil is huge by Paeonia suffruticosa seedling demand, the seed seedling-raising that breeding coefficient is big will become the main method of oil Paeonia suffruticosa seedling breeding.But seed of Paeonia suffruticosa has very strong hibernation feature, under field conditions (factors) seed of Paeonia suffruticosa after planting to the spring in next year or the 3rd year could germination and emergence, this hibernation feature of seed of Paeonia suffruticosa will seriously restrict the production of oil Paeonia suffruticosa seedling and then the impact oil fast development of Paeonia suffruticosa industry.Though having some at present about the technique study breaking seed of Paeonia suffruticosa dormancy, but the conclusion of different researcher existing very big-difference.
In this context, it is proposed to a kind of simple and effective oil seed of Paeonia suffruticosa breaking dormancy methods, it is one of oil Paeonia suffruticosa seedling factorial praluction urgent problem.
Summary of the invention
A kind of method that it is an object of the invention to provide simple and effective oil seed of Paeonia suffruticosa termination of diapause, the method can the dormancy of efficient solution oil removing seed of Paeonia suffruticosa, shorten seedling raise period, it is achieved the oil factorial praluction of Paeonia suffruticosa seedling.
To achieve these goals, present invention employs following technical scheme:
A kind of method of oil seed of Paeonia suffruticosa termination of diapause, comprises the following steps:
A, the oil seed of Paeonia suffruticosa embryo quick after-ripening stage
1) gathering when peony fruit pod is crab roe color fruit pod, spread out in indoor shady and cool place by the fruit pod gathered, thickness is less than 20 centimetres, and every day is stirred once up and down；
2) after fruit pod natural cracking, collecting the seed come off, remove and go mouldy or shrivelled seed, the full seed indoor sunk in water intaking carry out Stratificated treatment after drying in the shade 12 days；
3) seed after screening being mixed moisture mass ratio is 50～60% fresh river sands, according to river sand: the mass ratio mixing of seed=3:1, spreads out in indoor, and thickness, less than 40 centimetres, opens the heat radiation ditch of wide 20 centimetres every 1 meter；
4) keep seed and river sand mixture internal temperature less than 30 DEG C, keep certain humidity, be processed as 30 days, obtain embryo and complete the seed of after-ripening and the mixture of river sand；
The seed of Paeonia suffruticosa hypocotyl termination of diapause stage used by B, oil
1) indoor temperature of the seed Yu river sand mixture that complete kind of embryo after-ripening is controlled at 15 20 DEG C；
2) keep the certain humidity of river sand, be processed as 30 days, obtain the root length seed more than 4 centimetres and river sand mixture；
C, oil seed of Paeonia suffruticosa top-wall effect release the stage
1) indoor temperature of the root length seed more than 4 centimetres and river sand mixture is controlled below 10 DEG C；
2) period sprays plant growth regulator 100mg/L once to seed with river sand mixture；
3) keep the certain humidity of river sand, be processed as 30 days, so far complete oil seed of Paeonia suffruticosa During The Release of Dormancy.
As preferably, the method step A 1 of aforesaid a kind of oil seed of Paeonia suffruticosa termination of diapause) in, peony fruit pod is gathered, and when Paeonia suffruticosa shell crab roe color or brown, is accomplished by gathering when seed milk yellow or rufous.
As preferably, the method step A 2 of aforesaid a kind of oil seed of Paeonia suffruticosa termination of diapause) in, spread 7 15 days after fruit pod of gathering, fruit pod gradually becomes brown or black, most of fruit pods are ftractureed voluntarily and produce seed, and minority manually divests, and are removed by fruit pod and sort out seed.
As preferably, the method step A 4 of aforesaid a kind of oil seed of Paeonia suffruticosa termination of diapause) in, keeping seed heat radiation and the certain humidity of river sand is 60%.
As preferably, the method step B 2 of aforesaid a kind of oil seed of Paeonia suffruticosa termination of diapause) in, keep the certain humidity of river sand to be
As preferably, the method step C 3 of aforesaid a kind of oil seed of Paeonia suffruticosa termination of diapause) in, keeping the certain humidity of river sand is 60%.
As preferably, the method step C 2 of aforesaid a kind of oil seed of Paeonia suffruticosa termination of diapause) in, sprinkling plant growth regulator is GA3.
By above-mentioned process, can the dormancy of efficient solution oil removing seed of Paeonia suffruticosa.
Compared with prior art, the present invention has following useful technique effect:
Oil seed of Paeonia suffruticosa kind embryo after-ripening provided by the invention, hypocotyl termination of diapause and top-wall effect release method, including first stage (kind embryo after-ripening), second stage (hypocotyl termination of diapause) and phase III (top-wall effect releasing), the these three stage be combined with each other the complete oil seed of Paeonia suffruticosa termination of diapause system of formation one.
What the present invention adopted is fresh river sand, low price, and facility of drawing materials can not only obtain good termination of diapause effect, and greatly reduce production cost in process of production.
Accompanying drawing explanation
Fig. 1 is the oil of the present invention seed with Paeonia suffruticosa hypocotyl termination of diapause.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail, and the explanation of the invention is not limited.
Present invention firstly provides the first stage method of oil seed of Paeonia suffruticosa termination of diapause, i.e. a kind of method of oil quick after-ripening of seed of Paeonia suffruticosa embryo, comprise the following steps:
1, the gathering of peony fruit pod
Gathering of peony fruit pod to be gathered in good time in batches, can not too early or too late.Too early seed is immature, and quality is tender and moisture many, it is easy to go rotten or shrivelled；Seed easily comes off too late.General when Paeonia suffruticosa shell crab roe color or brown, it is accomplished by gathering when seed milk yellow or rufous.
2, peony fruit pod pretreatment
After peony fruit is gathered, the indoor in shady and cool ventilation should be spread, allow its after-ripening, be sure not to tan by the sun, otherwise seed one drying, germinating capacity will be lost.Spread thickness and do not exceed 20 centimetres, should stir once up and down every day, in case inner heat is gone rotten.Through 7 15 days, fruit pod gradually became brown or black, and most of fruit pods are ftractureed voluntarily, produce seed, and minority can manually divest, and is then removed by fruit pod, sorts out seed.
3, seed of Paeonia suffruticosa pretreatment
For eliminating the impact on germination percentage of the shrivelled seed, can putting in clear water by the seed sorted out, after soaking 1-2 hour, remove the foreign material floated on the surface and shrivelled seed, the full seed indoor sunk in water intaking carry out Stratificated treatment after drying in the shade 12 days.
4, the seed of screening is mixed moisture mass ratio by embryo Aging storage is 50 60% fresh river sands, according to river sand: the mass ratio mixing of seed=3:1, spread out in indoor, thickness does not exceed 40 centimetres, opens the heat radiation ditch of wide 20 centimetres every 1 meter, keeps river sand and seed mix internal temperature not to exceed 30 DEG C, it is processed as 30 days, period notes keeping the certain humidity of river sand, and humidity is 60%, obtains embryo and completes the seed of after-ripening and the mixture of river sand.
In the oil second stage method of seed of Paeonia suffruticosa termination of diapause, i.e. the oil method of seed of Paeonia suffruticosa hypocotyl termination of diapause, comprise the following steps:
Hypocotyl termination of diapause processed sand Tibetan after 30 days, and Paeonia suffruticosa embryo is basically completed After-ripening, now controlled, at 15 20 DEG C, to be processed as 30 days by spreading the seed indoor temperature with river sand mixture, and period notes keeping the certain humidity of river sand, and humidity is 60%.
In the oil phase III method of seed of Paeonia suffruticosa termination of diapause, the method that namely oil releases with seed of Paeonia suffruticosa top-wall effect, comprise the following steps:
Check that seed is taken root situation, shown in Fig. 1, when the root length when more than 80% is more than 4 centimetres, can control spreading the seed indoor temperature with river sand mixture below 10 DEG C；Period to seed with river sand mixture spray 100mg/LGA3 once, is processed as 30 days, and period notes keeping the certain humidity of river sand, and humidity is 60%.
By above-mentioned process, the seed breaking dormancy of more than 90% can be made.
Above content is in conjunction with concrete preferred implementation further description made for the present invention; it cannot be assumed that the specific embodiment of the present invention is only limitted to this; for general technical staff of the technical field of the invention; without departing from the inventive concept of the premise; some simple reasonings or replacement can also be made, all should be considered as belonging to the present invention and be determined scope of patent protection by submitted claims.
Claims (7)
1. the oil method of seed of Paeonia suffruticosa termination of diapause, it is characterised in that comprise the following steps:
A, the oil seed of Paeonia suffruticosa embryo quick after-ripening stage
1) gathering when peony fruit pod is crab roe color fruit pod, spread out in indoor shady and cool place by the fruit pod gathered, thickness is less than 20 centimetres, and every day is stirred once up and down；
2) after fruit pod natural cracking, collecting the seed come off, remove and go mouldy or shrivelled seed, the full seed indoor sunk in water intaking carry out Stratificated treatment after drying in the shade 12 days；
3) seed after screening being mixed moisture mass ratio is 50～60% fresh river sands, according to river sand: the mass ratio mixing of seed=3:1, spreads out in indoor, and thickness, less than 40 centimetres, opens the heat radiation ditch of wide 20 centimetres every 1 meter；
4) keep seed and river sand mixture internal temperature less than 30 DEG C, keep certain humidity, be processed as 30 days, obtain embryo and complete the seed of after-ripening and the mixture of river sand；
The seed of Paeonia suffruticosa hypocotyl termination of diapause stage used by B, oil
1) indoor temperature of the seed Yu river sand mixture that complete kind of embryo after-ripening is controlled at 15 20 DEG C；
2) keep the certain humidity of river sand, be processed as 30 days, obtain the root length seed more than 4 centimetres and river sand mixture；
C, oil seed of Paeonia suffruticosa top-wall effect release the stage
1) indoor temperature of the root length seed more than 4 centimetres and river sand mixture is controlled below 10 DEG C；
2) period sprays plant growth regulator 100mg/L once to seed with river sand mixture；
3) keep the certain humidity of river sand, be processed as 30 days, so far complete oil seed of Paeonia suffruticosa During The Release of Dormancy.
2. the oil method of seed of Paeonia suffruticosa termination of diapause described in 1 as requested, it is characterised in that described step A 1) in, peony fruit pod is gathered, and when Paeonia suffruticosa shell crab roe color or brown, is accomplished by gathering when seed milk yellow or rufous.
3. the oil method of seed of Paeonia suffruticosa termination of diapause described in 1 as requested, it is characterised in that described step A 2) in, gather fruit pod after spread 7 15 days, fruit pod gradually become brown or black, most of fruit pods are ftractureed voluntarily and produce seed, minority manually divests, and is removed by fruit pod and sorts out seed.
4. the oil method of seed of Paeonia suffruticosa termination of diapause described in 1 as requested, it is characterised in that described step A 4) in, keeping seed heat radiation and the certain humidity of river sand is 60%.
5. the oil method of seed of Paeonia suffruticosa termination of diapause described in 1 as requested, it is characterised in that described step B 2) in, keeping the certain humidity of river sand is 60%.
6. the oil method of seed of Paeonia suffruticosa termination of diapause described in 1 as requested, it is characterised in that described step C 3) in, keeping the certain humidity of river sand is 60%.
7. the oil method of seed of Paeonia suffruticosa termination of diapause described in 1 as requested, it is characterised in that described step C 2) in, sprinkling plant growth regulator is GA3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610141018.2A CN105766129A (en) | 2016-03-11 | 2016-03-11 | Method for releasing dormancy of oil-used peony seeds |
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| CN201610141018.2A CN105766129A (en) | 2016-03-11 | 2016-03-11 | Method for releasing dormancy of oil-used peony seeds |
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| CN105766129A true CN105766129A (en) | 2016-07-20 |
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| CN201610141018.2A Pending CN105766129A (en) | 2016-03-11 | 2016-03-11 | Method for releasing dormancy of oil-used peony seeds |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110235708A (en) * | 2019-07-25 | 2019-09-17 | 锡林郭勒盟蒙医医院 | A kind of wild Marsh Felwort introduction and acclimatization and cultivation mating system |
| CN110476535A (en) * | 2019-07-31 | 2019-11-22 | 河南科技大学 | A kind of implantation methods releasing ' Feng Dan ' tree peony suspend mode under natural conditions |
| CN114631459A (en) * | 2022-04-07 | 2022-06-17 | 云南省农业科学院高山经济植物研究所 | Method for quickly forming seedlings of paeonia suffruticosa seeds |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110235708A (en) * | 2019-07-25 | 2019-09-17 | 锡林郭勒盟蒙医医院 | A kind of wild Marsh Felwort introduction and acclimatization and cultivation mating system |
| CN110235708B (en) * | 2019-07-25 | 2022-02-25 | 锡林郭勒盟蒙医医院 | Wild intercostal flower introduction domestication and cultivation breeding method |
| CN110476535A (en) * | 2019-07-31 | 2019-11-22 | 河南科技大学 | A kind of implantation methods releasing ' Feng Dan ' tree peony suspend mode under natural conditions |
| CN110476535B (en) * | 2019-07-31 | 2022-02-15 | 河南科技大学 | Planting method for breaking dormancy of paeonia ostii peony in natural state |
| CN114631459A (en) * | 2022-04-07 | 2022-06-17 | 云南省农业科学院高山经济植物研究所 | Method for quickly forming seedlings of paeonia suffruticosa seeds |
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