CN109392638A - A kind of preparation method and cultural method of RADIX CURCUMAE detoxic seedling - Google Patents
A kind of preparation method and cultural method of RADIX CURCUMAE detoxic seedling Download PDFInfo
- Publication number
- CN109392638A CN109392638A CN201811276247.0A CN201811276247A CN109392638A CN 109392638 A CN109392638 A CN 109392638A CN 201811276247 A CN201811276247 A CN 201811276247A CN 109392638 A CN109392638 A CN 109392638A
- Authority
- CN
- China
- Prior art keywords
- seedling
- radix curcumae
- soil
- root
- detoxic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to the preparation methods and cultural method of a kind of RADIX CURCUMAE detoxic seedling, it is controlled by the condition to detoxic seedling preparation process, make that RADIX CURCUMAE detoxic seedling quality is higher and virus elimination rate almost can achieve 100%, in addition scientifically cultural method is provided, survival rate is close to 100% after making prepared detoxic seedling cultivation seedling-cultivating, and seedling is healthy and strong.
Description
Technical field
The present invention relates to the preparation methods and cultural method of a kind of RADIX CURCUMAE detoxic seedling.
Background technique
RADIX CURCUMAE (Curcuma wenyujin) is Zingiber curcuma, root tuber, rhizome can hyoscine, root tuber adds
Work at medicinal material RADIX CURCUMAE be one of famous genunie medicinal materials in Zhejiang Province " eight Zhe's ".For a kind of Zingiber of Southern Zhejiang area plantation
Medicinal plant, wild species are rare, and based on artificial cultivation, RADIX CURCUMAE likes warm and moist, sun-drenched weather, drought-enduring anti-
Flood, but be afraid of frost.It is suitable for being grown in the alluvial soil that the soil is porous, draining is good, soil layer is deep, sandy loam.RADIX CURCUMAE is bloomed
Seldom, seed does not enrich mostly, with kind of a race stem breeding in cultivation.RADIX CURCUMAE Annual growth cycle about 250 days or so, on general April
Ten days (clear and bright front and back) plantation, early or mid December harvest.
As industrial and agricultural production develops and promotes, soil pollution is got worse.Harmful heavy metal is run up to centainly in soil
Degree will be to soil -- and botanical system generates murder by poisoning, does not only result in soil degradation, crop yield and quality is directly caused to drop
It is low, jeopardize human life and health.Under the action of environmental factor, plant is mutually adapted with pathogen and mutually struggles against RADIX CURCUMAE
Lead to the generation and sprawling of disease.Since RADIX CURCUMAE belongs to more base sources kind, source is complicated, and the planting matrix of RADIX CURCUMAE at present
It is to be carried out according to traditional extensive mode in sheet, leads to variety deterioration, virusization seriously, harvests and the feelings such as Habitat producing is lack of standardization
Condition occurs.Cause quality of medicinal material unstable, it is difficult to be controlled, and then influence product quality, market competitiveness decline, market accounts for
There is share extension difficult.
Summary of the invention
The purpose of the invention is to overcome shortcoming and defect of the existing technology, and provide a kind of RADIX CURCUMAE detoxic seedling
Preparation method and cultural method.
The technical solution used in the present invention is as follows: a kind of preparation method of RADIX CURCUMAE detoxic seedling, comprising the following steps:
(1) select plant height in the short-stalked variety RADIX CURCUMAE of 70~120cm, take 0.5~2cm on RADIX CURCUMAE rhizomes terminal bud or
Axillary bud tip is rinsed 30-50 minutes with flowing water, then successively sterilizes 20-40s and 6-8 minutes with 70% ethyl alcohol and 10% hydrogen peroxide, so
It is rinsed again with sterile water 3 ~ 4 times afterwards；
(2) shoot tip meristem is taken, 0.1~0.3mm square is cut into, in MS+6-BA3.0mg/L+IAA1.0mg/L culture medium,
No light and temperature are cultivated 2 ~ 5 days under conditions of being 12 ~ 18 DEG C, are then 18 ~ 24 DEG C in temperature, daily illumination is the item of 4 ~ 6h
It is cultivated 15 ~ 20 days under part；
(3) it is forwarded to MS+2, is to carry out the induction of callus in temperature in 4-D2.0mg/L+KT5.0mg/L culture medium；Turn
It meets MS+2,4-D1.0mg/L+KT0.5mg/L and carries out squamous subculture；The MS+KT5.0mg/L+NAA0.3mg/L that transfers again is divided
Change；It takes root in MS+IAA1.0 mg/L, obtains detoxification test tube plantlet；
(4) it when needing to cultivate, opens detoxification test tube plantlet test tube bottleneck and is tamed for 3 ~ 5 days, disease then is carried out to detoxification test tube plantlet
Poison detection, selects not viruliferous plant, washes away culture medium, obtain RADIX CURCUMAE detoxic seedling.
A kind of cultural method of RADIX CURCUMAE detoxic seedling, comprising the following steps:
(1) rooting culture: establishing greenhouse area, first places bottle seedling in greenhouse 1-2 weeks before transplanting, when bottle outlet, by culture medium and group
Training seedling take out together, place clear water in wash culture medium, then clean 1-2 time with clear water again, for naked or lack root take off
Seedling root is placed in the ABT root-inducing powder of 100mg/L and impregnates 15min by malicious seedling；Then it transplants into matrix, matrix 30%-
50% perlite, 30%-50% vermiculite, 20%-35% length 2-3cm bark of dahurian larch mixed-matrix；After transplanting, watering, then
Nest is filled with 1500~2000 times of liquid trichoderma as biological microbial inoculums, then every the 1/4MS nutrient solution of spray in 3-5 days；
(2) soil treatment: selection loosing soil, the alluvial soil or sandy loam that draining is good, soil layer is deep, rich in organic matter, soil
Earth pH is neutral or detoxic seedling or prior year plantation gramineous crop, rape or sweet were not planted in subacidity, selection at least 3 years
Sugarcane, surrounding at least 500m are not planted the area of RADIX CURCUMAE, carry out disinfection, be ground into using the root of tripterygium wilfordii thin to soil
Powder applies in the soil after adding plant ash to mix；
(3) ridging plants seedling: 25~30cm of depth of raking the soil level breaks into the high ridge in 0.5~0.8m wide case face, to the seedling in step (1)
It is long to 6 ~ 8cm when, transplant into soil, seedling can be planted by disinfecting minimum 10 talent, and detoxic seedling base portion buries 3-4cm, watering, so
Nest is filled with 1500~2000 times of liquid trichoderma as biological microbial inoculums afterwards；
(4) management after transplanting: carrying out checking whether generation pest and disease damage every two days, to the seedling and dead seedling that pest and disease damage occurs
It is cleared up, keeps humidity in greenhouse to be greater than 65%, after two weeks, apply fertilizer, then water.
In step (2), the fine powder usage amount that the root of tripterygium wilfordii is ground into is per acre with 2.5~3.0kg, fine earth or plant ash
5.0~6.5kg.
In step (3), seedling spacing in the rows 10-15cm, line-spacing 0.65-1.05m.
In step (4), with decomposed rape cake, to extract after oil of zedoary turmeric the dregs of a decoction that generate with mass ratio be that 20-30:1 is smashed to pieces
Mixing, is applied fertilizer for 15 ~ 30 kilograms per acre by cave applying method.
Beneficial effects of the present invention are as follows: the detoxic seedling high survival rate for preparing and cultivating through the invention, and quality is good.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, the present invention will be made below further detailed
Description.
Embodiment one:
(1) when harvesting in autumn, plant height is selected in the short-stalked variety RADIX CURCUMAE of 70~120cm, during practicing planting experiment,
It was found that the growth of RADIX CURCUMAE cauline leaf is very luxuriant, surplus growth bring nutrient consumption is excessive, and the RADIX CURCUMAE kind of dwarfing not only resists
Lodging enhancing, promotes root growth, improves ventilation and penetrating light, reduces disease.The RADIX CURCUMAE main root stem for taking healthy growth, is removed
Soil and fibrous root are removed, is refrigerated 2 weeks in 4 DEG C of refrigerator, is embedded in the fine sand garden seedling through 50% carbendazim, 800 times of liquid immersion treatments,
And nursery is placed in vernalization under high temperature (36-40 DEG C), spire and leaf sheath are peelled off when bud length to 10cm or so, takes RADIX CURCUMAE root shape
The terminal bud of 0.5~2cm on stem or axillary bud tip are rinsed 30-50 minutes with flowing water, then successive with 70% ethyl alcohol and 10% hydrogen peroxide
Disinfection 20-40s and 6-8 minutes, is then rinsed 3 ~ 4 times again with sterile water.
(2) shoot tip meristem is taken, 0.1~0.3mm square is cut into, in MS+6-BA3.0mg/L+IAA1.0mg/L culture medium
In, it is cultivated under conditions of no light and temperature are 12 ~ 18 DEG C 2 ~ 5 days, is then 18 ~ 24 DEG C in temperature, daily illumination is 4 ~
It cultivates 15 ~ 20 days, is carried out from the induction and proliferation sprouted under conditions of 6h；
In test, by testing multiple and different culture mediums, last preferably MS+6-BA3.0mg/L+IAA1.0mg/L culture
Base, proliferation times are highest.
(3) it is forwarded to MS+2, is to carry out luring for callus in temperature in 4-D2.0mg/L+KT5.0mg/L culture medium
It leads, base portion expands, and bud point switchs to green, and plant induction rate is 72%；Transfer MS+2,4-D1.0mg/L+KT0.5mg/L carry out after
It is commissioned to train feeding, generates several small bud points；The MS+KT5.0mg/L+NAA0.3mg/L that transfers again is broken up, and bud point turns green, Plantlet Differentiation
Rate is 87%, bud well-grown；It takes root in MS+IAA1.0 mg/L, rooting rate reaches 94% or so, obtains detoxification test tube plantlet；
(4) it when needing to cultivate, opens detoxification test tube plantlet test tube bottleneck and is tamed for 3 ~ 5 days, disease then is carried out to detoxification test tube plantlet
Poison detection, selects not viruliferous plant, obtains RADIX CURCUMAE detoxic seedling, do not detect viruliferous plant, energy in viral diagnosis
Reach 100% virus elimination rate.
The detection mode of virus is as follows: taking a little blade of plant test tube seedling or root tissue with the tool after disinfection, is put into
In mortar, suitable distilled water is added and is ground.Leachate is dipped with copper mesh after plant tissue precipitating, is stayed a minute, is used
Filter paper sops up extraction raffinate from copper mesh edge.When the liquid on copper mesh will be dry not dry, 1 2% phosphotungstic acid negative staining liquid of drop is added dropwise, pays attention to
The copper mesh back side cannot be made to be stained with liquid, sop up extraction raffinate with filter paper after 1-2 minutes, only stay a small amount of liquid, spontaneously dried.With transmission electricity
The micro- sem observation virion of son.Each sample dips leachate with two copper mesh, and each copper mesh at least detects 20 or more no
The same visual field, and be control with non-detoxification sample.
Embodiment two:
(1) rooting culture: greenhouse area is established, is first placed bottle seedling in greenhouse 1-2 weeks before transplanting, to adapt to the temperature in nursery region
And sunshine, when bottle outlet, culture medium is taken out together with tissue-cultured seedling, places in clear water and wash culture medium, it is then clear with clear water again
It washes 1-2 times, for naked or the detoxic seedling of few root, seedling root is placed in the ABT root-inducing powder of 100mg/L and impregnates 15min；So
After transplant into matrix, matrix is the perlite of 30%-50%, 30%-50% vermiculite, 20%-35% length 2-3cm bark of dahurian larch
Mixed-matrix；After transplanting, then watering fills nest with 1500~2000 times of liquid trichoderma as biological microbial inoculums, then primary every spray in 3-5 days
1/4MS nutrient solution；
It is carried out by the mixed-matrix of the perlite of 30%-50%, 30%-50% vermiculite, 20%-35% length 2-3cm bark of dahurian larch
Nursery, when seedling it is long to 6 ~ 8cm when, stem is obviously more coarse, and blade quantity can achieve 6-8 piece, and nursery survival rate reaches
98% or so.
(2) soil treatment: selection loosing soil, the alluvial soil or sandy loam that draining is good, soil layer is deep, rich in organic matter
Soil, soil pH is neutral or detoxic seedling or prior year plantation gramineous crop, rape were not planted in subacidity, selection at least 3 years
Or sugarcane, surrounding at least 500m are not planted the area of RADIX CURCUMAE, carry out disinfection to soil, be ground into using the root of tripterygium wilfordii
Fine powder applies in the soil after adding plant ash to mix；
(3) ridging plants seedling: 25~30cm of depth of raking the soil level breaks into the high ridge in 0.5~0.8m wide case face, to the seedling in step (1)
It is long to 6 ~ 8cm when, transplant into soil, seedling can be planted by disinfecting minimum 10 talent, and detoxic seedling base portion buries 3-4cm, watering, so
Nest is filled with 1500~2000 times of liquid trichoderma as biological microbial inoculums afterwards；
(4) management after transplanting: carrying out checking whether generation pest and disease damage every two days, to the seedling and dead seedling that pest and disease damage occurs
It is cleared up, keeps humidity in greenhouse to be greater than 65%, after two weeks, apply fertilizer, then water.
It is that 20-30:1 smashs mixing to pieces that fertilising, which selects decomposed rape cake, extracts the dregs of a decoction generated after oil of zedoary turmeric with mass ratio,
It is applied fertilizer per acre by cave applying method for 15 ~ 30 kilograms.
It transplants after two weeks, seedling percent 98.91%.
After RADIX CURCUMAE seedling replanting to planting site, planted, disease incidence compare before reduction by 84%, single mu
Yield is compared to growth about 20%.
The above disclosure is only the preferred embodiments of the present invention, cannot limit the right model of the present invention with this certainly
It encloses, therefore equivalent changes made in accordance with the claims of the present invention, is still within the scope of the present invention.
Claims (5)
1. a kind of preparation method of RADIX CURCUMAE detoxic seedling, it is characterised in that the following steps are included:
Plant height is selected in the short-stalked variety RADIX CURCUMAE of 70~120cm, takes the terminal bud or armpit of 0.5~2cm on RADIX CURCUMAE rhizomes
Bud tip is rinsed 30-50 minutes with flowing water, then successively sterilizes 20-40s and 6-8 minutes with 70% ethyl alcohol and 10% hydrogen peroxide, then
It is rinsed 3-4 times again with sterile water；
Shoot tip meristem is taken, 0.1~0.3mm square is cut into, in MS+6-BA3.0mg/L+IAA1.0mg/L culture medium, in nothing
Illumination and temperature are cultivated 2 ~ 5 days under conditions of being 12-18 DEG C, are then 18-24 DEG C in temperature, daily illumination is the condition of 4-6h
Lower culture 15-20 days；
Budlet is forwarded to MS+2, is to carry out the induction of callus in temperature in 4-D2.0mg/L+KT5.0mg/L culture medium；
The MS+2,4-D1.0mg/L+KT0.5mg/L that transfers carries out squamous subculture；The MS+KT5.0mg/L+NAA0.3mg/L that transfers again is divided
Change；It takes root in MS+IAA1.0 mg/L, obtains detoxification test tube plantlet；
It when needing to cultivate, opens detoxification test tube plantlet test tube bottleneck 3-5 days and is tamed, viral inspection then is carried out to detoxification test tube plantlet
It surveys, selects not viruliferous plant, obtain RADIX CURCUMAE detoxic seedling.
2. a kind of cultural method of RADIX CURCUMAE detoxic seedling, it is characterised in that the following steps are included:
(1) rooting culture: establishing greenhouse area, first places bottle seedling in greenhouse 1-2 weeks before transplanting, when bottle outlet, by culture medium and group
Training seedling take out together, place clear water in wash culture medium, then clean 1-2 time with clear water again, for naked or lack root take off
Seedling root is placed in the ABT root-inducing powder of 100mg/L and impregnates 15min by malicious seedling；Then it transplants into matrix, matrix 30%-
50% perlite, 30%-50% vermiculite, 20%-35% length 2-3cm bark of dahurian larch mixed-matrix；After transplanting, watering, then
Nest is filled with 1500~2000 times of liquid trichoderma as biological microbial inoculums, then every the 1/4MS nutrient solution of spray in 3-5 days；
(2) soil treatment: selection loosing soil, the alluvial soil or sandy loam that draining is good, soil layer is deep, rich in organic matter, soil
Earth pH is neutral or detoxic seedling or prior year plantation gramineous crop, rape or sweet were not planted in subacidity, selection at least 3 years
Sugarcane, surrounding at least 500m are not planted the area of RADIX CURCUMAE, carry out disinfection, be ground into using the root of tripterygium wilfordii thin to soil
Powder applies in the soil after adding plant ash to mix；
(3) ridging plants seedling: 25~30cm of depth of raking the soil level breaks into the high ridge in 0.5~0.8m wide case face, to the seedling in step (1)
It is long to 6 ~ 8cm when, transplant into soil, seedling can be planted by disinfecting minimum 10 talent, and detoxic seedling base portion buries 3-4cm, watering, so
Nest is filled with 1500~2000 times of liquid trichoderma as biological microbial inoculums afterwards；
(4) management after transplanting: carrying out checking whether generation pest and disease damage every two days, to the seedling and dead seedling that pest and disease damage occurs
It is cleared up, keeps humidity in greenhouse to be greater than 65%, after two weeks, apply fertilizer, then water.
3. the cultural method of RADIX CURCUMAE detoxic seedling according to claim 2, it is characterised in that: in step (2), tripterygium wilfordii
The fine powder usage amount that root is ground into is per acre with 2.5~3.0kg, 5.0~6.5kg of fine earth or plant ash.
4. the cultural method of RADIX CURCUMAE detoxic seedling according to claim 2, it is characterised in that: in step (3), seedling spacing in the rows
10-15cm, line-spacing 0.65-1.05m.
5. the cultural method of RADIX CURCUMAE detoxic seedling according to claim 2, it is characterised in that: in step (4), with decomposed
The dregs of a decoction generated after rape cake, extraction oil of zedoary turmeric are that 20-30:1 smashs mixing to pieces with mass ratio, are applied per acre by cave for 15 ~ 30 kilograms
Method fertilising.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811276247.0A CN109392638A (en) | 2018-10-30 | 2018-10-30 | A kind of preparation method and cultural method of RADIX CURCUMAE detoxic seedling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811276247.0A CN109392638A (en) | 2018-10-30 | 2018-10-30 | A kind of preparation method and cultural method of RADIX CURCUMAE detoxic seedling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109392638A true CN109392638A (en) | 2019-03-01 |
Family
ID=65470087
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811276247.0A Pending CN109392638A (en) | 2018-10-30 | 2018-10-30 | A kind of preparation method and cultural method of RADIX CURCUMAE detoxic seedling |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109392638A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113966717A (en) * | 2021-11-10 | 2022-01-25 | 广西壮族自治区药用植物园 | Method for transplanting large curcuma zedoary tissue culture seedlings in greenhouse |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101103701A (en) * | 2007-08-06 | 2008-01-16 | 浙江大学 | Curcuma wenyujin detoxicating and quick reproduction method |
| CN101536639A (en) * | 2008-03-21 | 2009-09-23 | 温州医学院 | Curcuma wenyujin high yield culture technology |
| CN101953300A (en) * | 2010-08-24 | 2011-01-26 | 浙江大学 | Tissue culture method for Curcuma wenyujin No.1 |
| CN107410032A (en) * | 2017-09-26 | 2017-12-01 | 杭州师范大学 | A kind of method of RADIX CURCUMAE Regenerated Plantlets |
| CN107810811A (en) * | 2017-10-19 | 2018-03-20 | 大理药业股份有限公司 | A kind of method of tailored version RADIX CURCUMAE stock breeding |
-
2018
- 2018-10-30 CN CN201811276247.0A patent/CN109392638A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101103701A (en) * | 2007-08-06 | 2008-01-16 | 浙江大学 | Curcuma wenyujin detoxicating and quick reproduction method |
| CN101536639A (en) * | 2008-03-21 | 2009-09-23 | 温州医学院 | Curcuma wenyujin high yield culture technology |
| CN101953300A (en) * | 2010-08-24 | 2011-01-26 | 浙江大学 | Tissue culture method for Curcuma wenyujin No.1 |
| CN107410032A (en) * | 2017-09-26 | 2017-12-01 | 杭州师范大学 | A kind of method of RADIX CURCUMAE Regenerated Plantlets |
| CN107810811A (en) * | 2017-10-19 | 2018-03-20 | 大理药业股份有限公司 | A kind of method of tailored version RADIX CURCUMAE stock breeding |
Non-Patent Citations (1)
| Title |
|---|
| 汪洪 等: "温郁金脱毒组织培养技术研究", 《药物生物技术》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113966717A (en) * | 2021-11-10 | 2022-01-25 | 广西壮族自治区药用植物园 | Method for transplanting large curcuma zedoary tissue culture seedlings in greenhouse |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105393742A (en) | Open field ginger large-area seed propagation cultivation method | |
| CN103766128A (en) | Asexual rapid propagation method for idesia seedlings | |
| CN102939898B (en) | Method for catalpa bungei clonal seed selection | |
| CN105432290A (en) | Method for breeding peony seeds | |
| CN104067939A (en) | Tissue culture rapid propagation method of radix gentianae | |
| CN104322255B (en) | The culture apparatus of Rhizoma Zingiberis Recens | |
| CN105993816A (en) | Leaf picked ginkgo biloba and pinellia ternate interplanting cultivation method | |
| CN103141282B (en) | Cultivation method of panax ginseng | |
| CN103168690B (en) | Breeding method of Qi dioscorea opposita virus-free miniature seed beans | |
| CN104823855A (en) | Chirita brachytricha tissue culture and rapid propagation method | |
| CN104145644A (en) | Planting method of polygonatum odoratum | |
| CN104322254B (en) | The cultivation system of Rhizoma Solani tuber osi | |
| CN106613172B (en) | Original ecological planting method of anoectochilus roxburghii in Guangdong-oriented chemical-change lake sky and land | |
| CN104737766A (en) | Method for artificially cultivating resveratrol-enhanced polygonum cuspidatum through tissue culture | |
| CN107624467A (en) | A kind of fruit of a cubeb litsea tree method for culturing seedlings | |
| CN104170729A (en) | Method for obtaining hedychium coronarium tetraploid plants by slice culture technology | |
| CN101743908A (en) | Tissue culture, rapid propagation and cultivation method of grevillea banksii | |
| CN109392638A (en) | A kind of preparation method and cultural method of RADIX CURCUMAE detoxic seedling | |
| CN104871909A (en) | Cultivation method of Karst rock-desertification area gardenia jasminoides ellis | |
| CN104885762A (en) | Cultivation method of conservation rock-desertification area gardenia jasminoides ellis | |
| CN109169132A (en) | A kind of Hipeastrum vittalum (L Her.) Herb.- Amaryllisvittata Ait tissue-cultured seedling crop field efficient cultivation method | |
| CN204217520U (en) | The planting unit of sweet potato | |
| CN204217519U (en) | The culturing device of ginger | |
| CN104920000A (en) | Wild cultivation imitated method of lycopodium serratum at under-forest environment | |
| CN107616068A (en) | A kind of high efficiency, low cost potato virus-free plantlet and seed production technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190301 |
|
| RJ01 | Rejection of invention patent application after publication |