CN105723998B - A kind of polygerm paris polyphylla underground rhizome stripping and slicing mating system - Google Patents
A kind of polygerm paris polyphylla underground rhizome stripping and slicing mating system Download PDFInfo
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- CN105723998B CN105723998B CN201410764239.6A CN201410764239A CN105723998B CN 105723998 B CN105723998 B CN 105723998B CN 201410764239 A CN201410764239 A CN 201410764239A CN 105723998 B CN105723998 B CN 105723998B
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Abstract
The present invention provides a kind of methods of polygerm paris polyphylla underground rhizome stripping and slicing breeding, this method combines stripping and slicing raising technology with Dominant variety, the characteristic for making full use of polygerm paris polyphylla polygerm to sprout, using beveling method, and Stratification technique is utilized, promote Paris polyphylla underground rhizome surface resting bud to sprout as new plant.Proprietary formula soil is used simultaneously, keeps humiture and gas permeability appropriate, ensures without putrefaction in stripping and slicing pregermination procedure, to realize the quick industrialization of paris polyphylla.Stripping and slicing seedling is produced with the present invention, planting percent can reach 95%, and stripping and slicing loss is less than 5%, and 90% or more polygerm rate, 1 mu of Paris polyphylla can expand numerous 10 mu or more, and germination time is within 1 year, so may be implemented all to shift to an earlier date 23 years than seed seedling-raising for seedling in 1 year.The seedling stalwartness come out, well developed root system are bred, plant current year can blossom and bear fruit, and produce seed, medicinal material can be supplied by planting 5 years.
Description
Technical field
The invention belongs to Chinese medicine plantation raising technology field, it is related to a kind of polygerm paris polyphylla underground rhizome stripping and slicing breeding
Method.
Background technology
Paris polyphylla is Liliaceae paris plant, is the main of the drugs such as Gong Xue Ning, antiviral granule, jidesheng sheyao tablets
Raw material, because of the vigorous market demand, paris polyphylla wild resource is drastically reduced, endangered.Paris polyphylla growth cycle is long, manually
It is difficult to cultivate, and especially seedling breeding technical difficulty is larger, and seeling industry becomes the pass for restricting entire Paris polyphylla plantation industry
Key link, thus it is current, and technical staff puts into a large amount of time, energy and fund and carries out correlative study.Wherein more successfully
It is seed seedling-raising, but the growing-seedling period of seed seedling-raising is longer, sowing is just emerged for 2 years for the first time, and 3 ability of emergence are as seedling
Transplanting, entire growing-seedling period are up to 4 years, and management cost is higher, cause cost high.Tissue cultures are the weights of vegetative propagation
Means are wanted, people further investigate the tissue cultures of Paris polyphylla for many years, and explant callus induction group is acquired from each position
It knits, by the proliferation of callus and is differentiated to form new plant, though have current it has been reported that great still without obtaining
It breaks through, can be applied in actual production plantation.
It is one of current upper most common modes of reproduction of Paris polyphylla production using underground rhizome rhizome cutting propagation.The method both may be used
Quickly to breed Paris polyphylla seedling, and crop cycle can be shortened.Paris polyphylla underground rhizome is because of its section as material of nourishing and generating
On there are resting buds, when rhizome truncated, or because of stimulations such as wound, insect bites after, resting bud can be promoted to germinate, formed one
Or multiple new plant.
Number of patent application is that the patent application document of CN201310314995.4 discloses one kind from the induction of magnificent rhizoma paris rhizome
The technology of Multiple Buds fast asexual propagation.Specifically, the side that Multiple Buds fast asexual propagation is induced from magnificent rhizoma paris rhizome is disclosed
Method is：The magnificent rhizoma paris rhizome of choosing is spare, dries in the air 3-7 days, with cutter in rhizome midline incision, and by cutting part in 10-30ppm's
ABT rooting powder solution immersion treatments 2-10 hours, then notch is sterilized with plant ash, being used in combination plant ash to be mixed with clean river sand will be magnificent
Rhizoma paris rhizome is wrapped up in mix processing after, by strain transplant plant, above coated with the fine earth of thickness about 5mm；After rhizome plantation, Multiple Buds are from mother
Sprouted in strain, after 1 year or so time grew, each China Paris polyphylla maternal plant can produce more than 20 strain plantlets, bud, small rhizome,
Radicula is provided with, and is transplanted as magnificent Paris polyphylla seedling；Magnificent Paris polyphylla finished product is harvested after 3-4.Number of patent application is
The patent application document of CN201210364553.6 discloses a kind of method that magnificent Paris polyphylla seedling is quick, efficiently breeds.Specifically
The method that magnificent Paris polyphylla seedling is quick, efficiently breeds is disclosed, the selection and processing of propagating materials are included；It is induced using Stratificated treatment
Resting bud is sprouted.Sapling multiplication processing and production management.What the two patents were related to is the seedling breeding method of magnificent Paris polyphylla.
Number of patent application is that the patent application document of CN200710052465.1 discloses a kind of rhizome of long connective Paris polyphylla
Multiple Buds asexual reproduction method.Specifically, include the following steps：(a) selection of propagating materials；(b) the processing side of propagating materials
Method；(c) processing time of propagating materials, whole year can all carry out；(d) environment of induction Multiple Buds and management；(e) Multiple Buds seedling
Transplantation technique.
The patent application document that number of patent application is CN200810233590.7 discloses a kind of paris polyphylla stem tuber high power and cuts
Method for culturing seedlings is cut, specifically discloses and selects stalwartness, disease-free paris polyphylla stem tuber crosscutting at segment by stipes, then each small
Duan Zong is cut into small pieces, and ensures that each stripping and slicing carries stem trace, after stripping and slicing is put into the seedling bed for completing fertile soil or sandy soil, covers 3-
The fertile soil or sandy soil of 5cm, it is 50-60% to keep the relative humidity of soil, and the 2nd year 9-10 month, band bud stripping and slicing is in seedling bed
On be planted, keep soil relative humidity be 60-70%, by 40-60% degree of shading shade, temporary planting 1 year after transplanting greatly
Field.Using the present invention, paris polyphylla stem tuber germination rate is 85-90%, and rooting rate 40-50%, breeding coefficient is than conventional paris polyphylla
Rhizome segment seedling method improves 5 times or more.The patent is mainly that the terminal bud of paris polyphylla is cut off, and is not excavated, and use and be just located in
The method of reason.
Number of patent application is that the patent application document of CN200810058017.7 discloses Yunnan rhizoma paridis rhizocaul multiple germination raise-seedling
Method.It specifically discloses and 7-8 months the paris polyphylla plane that plantation survives is opened into soil until exposing bud, prescinded with the knife sterilized
Bud sterilizes paris polyphylla bud notch, with the limewash sterile perimeter soil of a concentration of 1-5 ‰, with a concentration of 80- with plant ash
The Gibberellins solution of 120mg/L is sprayed at paris polyphylla top exposed portion, after drying in the air 4-7 days, the fine earth of covering thickness 5-8mm, and sprinkling
After a concentration of 40-60%ABT rooting powder solutions, then cover the detritus soil and soil of thick 5-8cm.Although the patent application is to excavation
Rhizome stripping and slicing, but the method for using secondary temporary planting.
Number of patent application is that the patent application document of CN200710066296.7 discloses a kind of Yunnan Rhizoma Paridis rhizome segment
Method for culturing seedlings.It specifically discloses and uses Yunnan Rhizoma Paridis rhizome segment in the 3-4 months or the 9-10 months, with the cell division of 2-10mg/L
Plain 6-BA impregnates 12-36 hours, and one layer of loam, one layer of segment indoors is placed, soil layer thickness 3-5cm, the total height 10- of stacking
50cm moves on to greenhouse, by seeding row spacing 2-5*2- when having root 1-3 after keeping loam relative water content 50-70%, adventitious bud to sprout
5cm high density is placed on greenhouse seedbed, and seedling can a plant division work kind transplantation of seedlings crop field within 2 years.The patent application document cuts excavation rhizome
Block is impregnated using root-inducing powder, the method for secondary temporary planting.
But there is no the raising technologies for being directed to polygerm paris polyphylla in the prior art.
Invention content
In order to make up for the deficiencies of the prior art, the present invention provides a kind of sides of polygerm paris polyphylla underground rhizome stripping and slicing breeding
Method, this method combine stripping and slicing raising technology with Dominant variety, and the characteristic for making full use of polygerm paris polyphylla polygerm to sprout is adopted
With beveling method, and utilize Stratification technique, promote Paris polyphylla underground rhizome surface resting bud sprout become new plant.Simultaneously using special
With formula soil, temperature, humidity and gas permeability appropriate are kept, ensures stripping and slicing without putrefaction in pregermination procedure, to realize
The quick industrialization of paris polyphylla.
On the one hand, the present invention provides a kind of polygerm paris polyphylla underground rhizome stripping and slicing breeding method, the method includes
Following steps：
1) it excavates：
Introduces a collection of the polygerm paris polyphylla of 3-5 as stripping and slicing is selected, after waiting for the seed maturity harvesting of the month in autumn 9-11, excavation
Underground rhizome, the material as stripping and slicing breeding；
Preferably, time of the introduces a collection from excavation to stripping and slicing is no more than 24 hours；It is highly preferred that the introduces a collection is on the spot
The polygerm paris polyphylla of excavation；
2) stripping and slicing：By the oblique knife stripping and slicing of the bud head of the underground rhizome, wherein 2-3cm at the most side of bud head root to incisxal edge, tail
Portion is cut to the not equal stripping and slicing of 2-3cm length and width, and each piece upper with epidermis；
Preferably, the fresh polygerm paris polyphylla rhizome of per kilogram is cut to 30-200 stripping and slicing；It is highly preferred that by per kilogram
Fresh polygerm paris polyphylla rhizome is cut to 50-100 stripping and slicing；
Preferably, the part of epidermis and part chopped will not dry as medicinal material in the stripping and slicing；
3) it sterilizes for the first time：It is prepared as liquid medicine using carbendazim, phoxim, mashed garlic, water, the polygerm paris polyphylla that will be cut
Stripping and slicing is impregnated 0.5-3 hour using liquid medicine, and the liquid medicine dosage is to flood polygerm paris polyphylla stripping and slicing, then drains and dries table
Face moisture；
Wherein, the mass ratio of carbendazim in the liquid medicine, phoxim, mashed garlic and water is 2-5：3-6：120-160：400-
600；
4) it sterilizes for second：The polygerm paris polyphylla stripping and slicing shone is stirred evenly with the mixture of stove ash and carbendazim, is dried in the air
It shines 0.5-3 hours, to promote the section organization healing of polygerm paris polyphylla stripping and slicing；
Wherein, the stove ash is remaining ashes after firewood weeds completely burned；
Wherein, the mass ratio of the mixture medium mess ash and carbendazim of the stove ash and carbendazim is 100-150:1-4；
5) sand storage：Shady and cool the sand of upper paving 1-5cm, the polygerm paris polyphylla stripping and slicing disinfected is laid on sand
Then face covers 1-5cm with sand, then in one layer of polygerm paris polyphylla stripping and slicing of tiling above, every layer of Paris polyphylla is no more than 4cm, layering
Pile, sand storage 5-9 days；
After sand storage, plants the polygerm paris polyphylla stripping and slicing with bud head as seedling, cut without the polygerm paris polyphylla of bud head
Block carries out vernalization processing；
Preferably, the number of plies of the layering pile is no more than 5 layers；
6) vernalization：The polygerm paris polyphylla stripping and slicing without bud head after sand storage is subjected to vernalization：
First, the vernalization formula soil that 3-6cm is spread in bottom is turned over once, and level land is sprinkled into carbendazim and phoxim, then
With plank applanation surface；
Wherein, 4-15 grams of carbendazim, 5-20 grams of phoxim are sprinkled into for every square metre；
Secondly, the vernalization formula soil of 2-4cm is repaved on the surface, the polygerm paris polyphylla then put without bud head is cut
Block, the stripping and slicing are not carried against together；It so repeats, puts 4 layers of polygerm paris polyphylla stripping and slicing；
Finally, the vernalization formula soil of one layer of paving 8-12cm, covers the pine tag of 3-6cm above above；
Wherein, the mountain base soil that mass fraction is 30-100% is contained in the vernalization formula soil；
Wherein, the condition of the vernalization is to keep the humidity of soil 50-70%, and air themperature is not above 30 degree, vernalization
Time is usually 6-12 months, after new bud head and fibrous root are longer, that is, pregermination procedure is completed, without the polygerm Yunnan of bud head
Paris polyphylla stripping and slicing can be used as seedling to plant.
Preferably, the polygerm paris polyphylla is the complete No. 1 polygerm paris polyphylla of cloud, and it is limited can be purchased from the full biology exploitation of Lijing cloud
Company；
In the preferred embodiment of the present invention, it the described method comprises the following steps：
A. introduces a collection is selected：The complete No. 1 polygerm paris polyphylla of cloud that selection has grown 3-5 makees introduces a collection；Wait for that the month in autumn 9-11 plants
After sub ripe harvesting, underground rhizome is excavated, epidermis sandy soil are shaken off；
B. stripping and slicing：In 24 hours, by the oblique knife stripping and slicing of the bud head of the underground rhizome, wherein bud head root to incisxal edge most
2-3cm at side, tail portion are cut to the not equal stripping and slicing of 2-3cm length and width, and each piece upper with epidermis, the fresh polygerm paris polyphylla of per kilogram
Rhizome is cut to 30-200 stripping and slicing；Preferably, the fresh polygerm paris polyphylla rhizome of per kilogram is cut to 50-100 stripping and slicing
C. it sterilizes for the first time：It is prepared as liquid medicine using carbendazim, phoxim, mashed garlic, water, the polygerm paris polyphylla that will be cut
Stripping and slicing is impregnated 0.5-3 hours using liquid medicine, and the liquid medicine dosage is to flood polygerm paris polyphylla stripping and slicing, then drains and dries surface
Moisture；
Wherein, the mass ratio of carbendazim in the liquid medicine, phoxim, mashed garlic and water is 3-4：4-5：140-150：450-
550；
D. it sterilizes for second：The polygerm paris polyphylla stripping and slicing shone is stirred evenly with the mixture of stove ash and carbendazim, is dried in the air
It shines 0.5-3 hours, to promote the section organization healing of polygerm paris polyphylla stripping and slicing；
Wherein, the mass ratio of the mixture medium mess ash and carbendazim of the stove ash and carbendazim is 120-140：2-3；
E. sand storage：Shady and cool the sand of upper paving 2-4cm, the polygerm paris polyphylla stripping and slicing disinfected is laid on sand
Then face covers 2-4cm with sand, then in one layer of polygerm paris polyphylla stripping and slicing of tiling above, every layer of Paris polyphylla is no more than 4cm, layering
Pile, no more than 5 layers, sand storage 7-8 days；
After sand storage, plants the polygerm paris polyphylla stripping and slicing with bud head as seedling, cut without the polygerm paris polyphylla of bud head
Block carries out vernalization processing；
F. vernalization：The polygerm paris polyphylla stripping and slicing without bud head after sand storage is subjected to vernalization：
First, the vernalization formula soil that 4-5cm is spread in bottom is turned over once, and level land is sprinkled into carbendazim and phoxim, then
With plank applanation surface；
Wherein, 8-12 grams of carbendazim, 10-15 grams of phoxim are sprinkled into for every square metre；
Secondly, the vernalization formula soil of 2-4cm is repaved on the surface, the polygerm paris polyphylla then put without bud head is cut
Block, the stripping and slicing are not carried against together；It so repeats, puts 4 layers of polygerm paris polyphylla stripping and slicing；
Finally, the vernalization formula soil of one layer of paving 8-12cm, covers the pine tag of 3-6cm above above；
Wherein, the vernalization formula soil contains the mountain base soil that mass fraction is 30-100%；
Wherein, the condition of the vernalization is to keep the humidity of soil 50-70%, and air themperature is not above 30 degree, vernalization
Time is usually 6-12 months, after new bud head and fibrous root are longer, that is, pregermination procedure is completed, without the polygerm Yunnan of bud head
Paris polyphylla stripping and slicing can be used as seedling to plant.
The inventors of the present application found that using polygerm paris polyphylla as stripping and slicing introduces a collection, suitable season, appropriate place are selected
Reason method promotes the sprouting of bud head and new root system it is possible to prevente effectively from stripping and slicing rhizome goes mouldy and rots, and shortens vernalization
Journey improves planting percent, reduces loss, and breeding coefficient is higher, still keeps polygerm head character, such seedling underground rhizome growth fast
Speed, root yield are higher.Stripping and slicing seedling is produced with the present invention, planting percent can reach 95%, and stripping and slicing loss is less than 5%, polygerm rate
90% or more, 1 mu of Paris polyphylla can expand numerous 10 mu or more, and germination time is within 1 year, so may be implemented in 1 year all for seedling, than
Seed seedling-raising will shift to an earlier date 2-3.And because seedling is healthy and strong, well developed root system can blossom and bear fruit after plantation, can effectively improve kind
Benefit is planted, expands numerous population quantity using self-produced seed, realizes quick industrialization.The seedling stalwartness come out, well developed root system are bred, is planted
Strain current year can blossom and bear fruit, and produce seed, and plantation can supply medicinal material in 5 years.
The present invention combines stripping and slicing raising technology with Dominant variety, the spy for making full use of polygerm paris polyphylla polygerm to sprout
Property, loss is utmostly reduced using beveling method, stripping and slicing pregermination procedure does not use any basic element of cell division, easy to operate, cost
It is cheap, the simple chemical agent for meeting specification is only used, the toxic side effect of medicinal material will not be increased, the chemistry of medicinal material will not be increased
Residual.
Specific implementation mode
With reference to embodiment, the present invention is described further, but not limited to the examples.
Embodiment 1
Introduces a collection lot number is 20121015JD, and introduces a collection is that the towns Ju Dian Lijing cloud plants polygerm in biological development corporation, Ltd. base entirely
Paris polyphylla " complete No. 1 of cloud ", Planting Years are 5 years, and the excavation time is on October 15th, 2012, and concrete operation step is as follows：
A. introduces a collection is selected：Selecting Lijing cloud, 5 years polygerm paris polyphyllas " complete No. 1 of cloud " 1 are planted by biological development corporation, Ltd. entirely
Mu；
B. it excavates：On October 15th, 2012 excavates underground rhizome after harvesting seed, shakes off epidermis sandy soil, 1100 public affairs of weighing
Jin transports stripping and slicing；
C. stripping and slicing：The paris polyphylla of excavation is cut into 77000 fritters, wherein bud head part is 23100 pieces, 53900 pieces of tail portion
D. it sterilizes：The polygerm paris polyphylla stripping and slicing cut is divided into 5 parts, is configured to carbendazim, phoxim, mashed garlic, water
Liquid medicine impregnates 1 hour, drains, dries surface moisture.By the mixture of the polygerm paris polyphylla stripping and slicing stove ash and carbendazim that have shone
It stirs evenly, dries 1 hour；
Wherein, the mass ratio of carbendazim, phoxim, mashed garlic and water is 2：3：120：400；
The mass ratio of stove ash and carbendazim is 100：4；
E. sand storage：It is shady and cool upper to spread 2cm thickness sands, the polygerm paris polyphylla stripping and slicing disinfected is laid in above sand,
Then 2cm being covered with sand, then in one layer of polygerm paris polyphylla stripping and slicing of tiling above, every layer of polygerm paris polyphylla stripping and slicing is no more than 4cm,
So layering pile, 5 layers in total, sand storage 7 days；After sand storage, the polygerm paris polyphylla stripping and slicing for containing bud head is planted as seedling, no
Polygerm paris polyphylla stripping and slicing with bud head carries out vernalization processing；
F. vernalization：Complete 1 point of ground is selected in No. 2 greenhouses of company, first spreads 5cm vernalization formula soils in bottom, is turned over once,
Level land is sprinkled into 333.5 grams of 226.8 grams of carbendazim and phoxim, with plank applanation surface.3cm vernalization formula soils, pendulum are spread in surface layer
Polygerm paris polyphylla stripping and slicing stem tuber is put, is not leaned on together between stem tuber, spreads 3cm vernalization formula soils, then put one layer of Paris polyphylla block above
Stem repaves 3cm vernalization formula soils, so repeats, and puts 4 layers of polygerm paris polyphylla stripping and slicing altogether, topmost one layer of paving 10cm vernalization formula
Soil, surrounding is slightly higher, covers 5cm pine tags above.Keep ventilated in canopy, temperature opens side window in time when being higher than 30 degree, increases
Add air to flow, while using spray, reducing temperature as early as possible.Winter is suitably warming.
Plant first band bud head seedling on October 22nd, 2012, in July, 2013, statistics emergence reached 98%；2013 12
Seedling of the transplanting of the moon 28 Jing Guo vernalization, on May 6th, 2014, statistics emergence rate reached 85%, and part bud head of not emerging is intact.It should
70 pieces of batch weight building per kilogram stripping and slicing, dissecting portion are 2.8 kilograms, and the seedling sum that vernalization comes out is 52013 plants, and planting percent is
96.5%.
Embodiment 2
Introduces a collection lot number is 20121015JD, and introduces a collection is that the towns Ju Dian Lijing cloud plants polygerm in biological development corporation, Ltd. base entirely
Paris polyphylla " complete No. 1 of cloud ", Planting Years are 5 years, and the excavation time is on October 15th, 2012, and concrete operation step is as follows：
A. introduces a collection is selected：Selecting Lijing cloud, 5 years polygerm paris polyphyllas " complete No. 1 of cloud " 1 are planted by biological development corporation, Ltd. entirely
Mu；
B. it excavates：On October 15th, 2012 excavates underground rhizome after harvesting seed, shakes off epidermis sandy soil, 1100 public affairs of weighing
Jin transports stripping and slicing；
C. stripping and slicing：The paris polyphylla of excavation is cut into 32200 fritters, wherein bud head part is 9900 pieces, 22300 pieces of tail portion
D. it sterilizes：The polygerm paris polyphylla stripping and slicing cut is divided into 5 parts, is configured to carbendazim, phoxim, mashed garlic, water
Liquid medicine impregnates 0.5 hour, drains, dries surface moisture.By the mixing of the polygerm paris polyphylla stripping and slicing stove ash and carbendazim that have shone
Object stirs evenly, and dries 0.5 hour；
Wherein, the mass ratio of carbendazim, phoxim, mashed garlic and water is 2：3：120：400；
The mass ratio of stove ash and carbendazim is 100：1；
E. sand storage：Shady and cool the sand of upper paving 5cm thickness, the polygerm paris polyphylla stripping and slicing disinfected is laid on sand
Then face covers 5cm with sand, then in one layer of polygerm paris polyphylla stripping and slicing of tiling above, every layer of polygerm paris polyphylla stripping and slicing is no more than
4cm is so layered pile, 5 layers in total, sand storage 5 days；After sand storage, the polygerm paris polyphylla stripping and slicing of bud head will be contained as kind of a seed
It plants, the polygerm paris polyphylla stripping and slicing without bud head carries out vernalization processing；
F. vernalization：Complete 1 point of ground is selected in No. 2 greenhouses of company, first spreads 3cm vernalization formula soils in bottom, is turned over once,
Level land is sprinkled into 333.5 grams of 226.8 grams of carbendazim and phoxim, with plank applanation surface.2cm vernalization formula soils, pendulum are spread in surface layer
Polygerm paris polyphylla stripping and slicing stem tuber is put, is not leaned on together between stem tuber, spreads 2cm vernalization formula soils, then put one layer of Paris polyphylla block above
Stem repaves 2cm vernalization formula soils, so repeats, and puts 4 layers of polygerm paris polyphylla stripping and slicing altogether, topmost one layer of paving 8cm vernalization formula
Soil, surrounding is slightly higher, covers 3cm pine tags above.Keep ventilated in canopy, temperature opens side window in time when being higher than 30 degree, increases
Add air to flow, while using spray, reducing temperature as early as possible.Winter is suitably warming.
Plant first band bud head seedling on October 22nd, 2012, in July, 2013, statistics emergence reached 99%；2013 12
Seedling of the transplanting of the moon 28 Jing Guo vernalization, on May 6th, 2014, statistics emergence rate reached 98%, and part bud head of not emerging is intact.It should
30 pieces of batch weight building per kilogram stripping and slicing, dissecting portion are 26.6 kilograms, and the seedling sum that vernalization comes out is 31110 plants, and planting percent is
98.3%.
Embodiment 3
Introduces a collection lot number is 20121015JD, and introduces a collection is that the towns Ju Dian Lijing cloud plants polygerm in biological development corporation, Ltd. base entirely
Paris polyphylla " complete No. 1 of cloud ", Planting Years are 5 years, and the excavation time is on October 15th, 2012, and concrete operation step is as follows：
A. introduces a collection is selected：Selecting Lijing cloud, 5 years polygerm paris polyphyllas " complete No. 1 of cloud " 1 are planted by biological development corporation, Ltd. entirely
Mu；
B. it excavates：On October 15th, 2012 excavates underground rhizome after harvesting seed, shakes off epidermis sandy soil, 1100 public affairs of weighing
Jin transports stripping and slicing；
C. stripping and slicing：The paris polyphylla of excavation is cut into 215400 fritters, wherein bud head part is 66000 pieces, tail portion 149400
Block
D. it sterilizes：The polygerm paris polyphylla stripping and slicing cut is divided into 10 parts, is configured to carbendazim, phoxim, mashed garlic, water
Liquid medicine impregnates 3 hours, drains, dries surface moisture.By the mixture of the polygerm paris polyphylla stripping and slicing stove ash and carbendazim that have shone
It stirs evenly, dries 3 hours；
Wherein, the mass ratio of carbendazim, phoxim, mashed garlic and water is 5：6：160：600；
The mass ratio of stove ash and carbendazim is 150：4；
E. sand storage：It is shady and cool upper to spread 5cm thickness sands, the polygerm paris polyphylla stripping and slicing disinfected is laid in above sand,
Then 5cm being covered with sand, then in one layer of polygerm paris polyphylla stripping and slicing of tiling above, every layer of polygerm paris polyphylla stripping and slicing is no more than 4cm,
So layering pile, 5 layers in total, sand storage 9 days；After sand storage, the polygerm paris polyphylla stripping and slicing for containing bud head is planted as seedling, no
Polygerm paris polyphylla stripping and slicing with bud head carries out vernalization processing；
F. vernalization：Complete 1 point of ground is selected in No. 2 greenhouses of company, first spreads 6cm vernalization formula soils in bottom, is turned over once,
Level land is sprinkled into 1334 grams of 1000.5 grams of carbendazim and phoxim, with plank applanation surface.4cm vernalization formula soils, pendulum are spread in surface layer
Polygerm paris polyphylla stripping and slicing stem tuber is put, is not leaned on together between stem tuber, spreads 4cm vernalization formula soils, then put one layer of Paris polyphylla block above
Stem repaves 4cm vernalization formula soils, so repeats, and puts 4 layers of polygerm paris polyphylla stripping and slicing altogether, topmost one layer of paving 12cm vernalization formula
Soil, surrounding is slightly higher, covers 6cm pine tags above.Keep ventilated in canopy, temperature opens side window in time when being higher than 30 degree, increases
Add air to flow, while using spray, reducing temperature as early as possible.Winter is suitably warming.
Plant first band bud head seedling on October 22nd, 2012, in July, 2013, statistics emergence reached 98.5%；2013
Transplanted the seedling Jing Guo vernalization on December 28, on May 6th, 2014, statistics emergence rate reached 97.5%, and part bud head of not emerging is complete
It is good.200 pieces of the batch weight building per kilogram stripping and slicing, dissecting portion are 23 kilograms, and the seedling sum that vernalization comes out is 210675 plants, seedling
Rate is 97.8%.
Claims (10)
1. a kind of polygerm paris polyphylla underground rhizome stripping and slicing mating system, the described method comprises the following steps：
1) it excavates：
Introduces a collection of the polygerm paris polyphylla of 3-5 as stripping and slicing is selected, after waiting for the seed maturity harvesting of the month in autumn 9-11, excavates underground
Rhizome, the material as stripping and slicing breeding；
2) stripping and slicing：By the oblique knife stripping and slicing of the bud head of the underground rhizome, wherein 2-3cm at the most side of bud head root to incisxal edge, tail portion is cut
For the not equal stripping and slicing of 2-3cm length and width, each piece upper with epidermis；
3) it sterilizes for the first time：It is prepared as liquid medicine using carbendazim, phoxim, mashed garlic, water, the polygerm paris polyphylla stripping and slicing that will be cut
It is impregnated 0.5-3 hours using liquid medicine, the liquid medicine dosage is to flood polygerm paris polyphylla stripping and slicing, then drains and dries surface water
Point；
Wherein, the mass ratio of carbendazim in the liquid medicine, phoxim, mashed garlic and water is 2-5：3-6：120-160：400-600；
4) it sterilizes for second：The polygerm paris polyphylla stripping and slicing shone is stirred evenly with the mixture of stove ash and carbendazim, is dried
0.5-3 hours, to promote the section organization healing of polygerm paris polyphylla stripping and slicing；
Wherein, the stove ash is remaining ashes after firewood weeds completely burned；
Wherein, the mass ratio of the mixture medium mess ash and carbendazim of the stove ash and carbendazim is 100-150：1-4；
5) sand storage：Shady and cool the sand of upper paving 1-5cm, the polygerm paris polyphylla stripping and slicing disinfected is laid in above sand, so
1-5cm being covered with sand afterwards, then in one layer of polygerm paris polyphylla stripping and slicing of tiling above, every layer of Paris polyphylla is no more than 4cm, is layered pile,
Sand storage 5-9 days；
After sand storage, using the polygerm paris polyphylla stripping and slicing with bud head as seedling plant, without bud head the stripping and slicing of polygerm paris polyphylla into
Row vernalization is handled；
6) vernalization：The polygerm paris polyphylla stripping and slicing without bud head after sand storage is subjected to vernalization：
First, the vernalization formula soil that 3-6cm is spread in bottom is turned over once, and level land is sprinkled into carbendazim and phoxim, then uses wood
Plate applanation surface；
Wherein, 4-15 grams of carbendazim, 5-20 grams of phoxim are sprinkled into for every square metre；
Secondly, the vernalization formula soil of 2-4cm is repaved on the surface, then puts the polygerm paris polyphylla stripping and slicing without bud head, institute
Stripping and slicing is stated to be not carried against together；It so repeats, puts 4 layers of polygerm paris polyphylla stripping and slicing；
Finally, the vernalization formula soil of one layer of paving 8-12cm, covers the pine tag of 3-6cm above above；
Wherein, the mountain base soil that mass fraction is 30-100% is contained in the vernalization formula soil；
Wherein, the condition of the vernalization is to keep the humidity of soil 50-70%, and air themperature is not above 30 degree, germination time
Usually 6-12 months, after new bud head and fibrous root are longer, that is, pregermination procedure is completed, without the polygerm paris polyphylla of bud head
Stripping and slicing can be used as seedling to plant.
2. according to the method described in claim 1, it is characterized in that, introduces a collection described in the step 1) from excavation to stripping and slicing when
Between be no more than 24 hours.
3. according to the method described in claim 1, it is characterized in that, introduces a collection described in the step 1) is the polygerm excavated on the spot
Paris polyphylla.
4. according to the method described in claim 1, it is characterized in that, the fresh polygerm paris polyphylla root of per kilogram in the step 2)
Stem is cut to 30-200 stripping and slicing.
5. according to the method described in claim 1, it is characterized in that, the fresh polygerm paris polyphylla root of per kilogram in the step 2)
Stem is cut to 50-100 stripping and slicing.
6. according to the method described in claim 1, it is characterized in that, in the step 2), will not there is no epidermis in the stripping and slicing
Part and part chopped are dried as medicinal material.
7. according to the method described in claim 1, it is characterized in that, in the step 5), the number of plies for being layered pile is no more than 5
Layer.
8. according to the method described in claim 1, it is characterized in that, the polygerm paris polyphylla is the complete No. 1 polygerm paris polyphylla of cloud.
9. according to the method described in any one of claim 1-8, which is characterized in that the described method comprises the following steps：
A. introduces a collection is selected：The complete No. 1 polygerm paris polyphylla of cloud that selection has grown 3-5 makees introduces a collection；Wait for the month in autumn 9-11 seed at
After ripe harvesting, underground rhizome is excavated, epidermis sandy soil are shaken off；
B. stripping and slicing：In 24 hours, by the oblique knife stripping and slicing of the bud head of the underground rhizome, wherein at the most side of bud head root to incisxal edge
2-3cm, tail portion are cut to the not equal stripping and slicing of 2-3cm length and width, and each piece upper with epidermis, the fresh polygerm paris polyphylla rhizome of per kilogram
It is cut to 30-200 stripping and slicing；
C. it sterilizes for the first time：It is prepared as liquid medicine using carbendazim, phoxim, mashed garlic, water, the polygerm paris polyphylla stripping and slicing that will be cut
It is impregnated 0.5-3 hours using liquid medicine, the liquid medicine dosage is to flood polygerm paris polyphylla stripping and slicing, then drains and dries surface water
Point；
Wherein, the mass ratio of carbendazim in the liquid medicine, phoxim, mashed garlic and water is 3-4：4-5：140-150：450-550；
D. it sterilizes for second：The polygerm paris polyphylla stripping and slicing shone is stirred evenly with the mixture of stove ash and carbendazim, is dried
0.5-3 hours, to promote the section organization healing of polygerm paris polyphylla stripping and slicing；
Wherein, the mass ratio of the mixture medium mess ash and carbendazim of the stove ash and carbendazim is 120-140：2-3；
E. sand storage：Shady and cool the sand of upper paving 2-4cm, the polygerm paris polyphylla stripping and slicing disinfected is laid in above sand, so
2-4cm being covered with sand afterwards, then in one layer of polygerm paris polyphylla stripping and slicing of tiling above, every layer of Paris polyphylla is no more than 4cm, is layered pile,
No more than 5 layers, sand storage 7-8 days；
After sand storage, using the polygerm paris polyphylla stripping and slicing with bud head as seedling plant, without bud head the stripping and slicing of polygerm paris polyphylla into
Row vernalization is handled；
F. vernalization：The polygerm paris polyphylla stripping and slicing without bud head after sand storage is subjected to vernalization：
First, the vernalization formula soil that 4-5cm is spread in bottom is turned over once, and level land is sprinkled into carbendazim and phoxim, then uses wood
Plate applanation surface；
Wherein, 8-12 grams of carbendazim, 10-15 grams of phoxim are sprinkled into for every square metre；
Secondly, the vernalization formula soil of 2-4cm is repaved on the surface, then puts the polygerm paris polyphylla stripping and slicing without bud head, institute
Stripping and slicing is stated to be not carried against together；It so repeats, puts 4 layers of polygerm paris polyphylla stripping and slicing；
Finally, the vernalization formula soil of one layer of paving 8-12cm, covers the pine tag of 3-6cm above above；
Wherein, the vernalization formula soil contains the mountain base soil that mass fraction is 30-100%；
Wherein, the condition of the vernalization is to keep the humidity of soil 50-70%, and air themperature is not above 30 degree, germination time
Usually 6-12 months, after new bud head and fibrous root are longer, that is, pregermination procedure is completed, without the polygerm paris polyphylla of bud head
Stripping and slicing can be used as seedling to plant.
10. according to the method described in claim 9, it is characterized in that, the polygerm Yunnan weight that per kilogram is fresh in step b. strippings and slicings
Building rhizome is cut to 50-100 stripping and slicing.
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| CN107242012A (en) * | 2017-07-26 | 2017-10-13 | 广西桂平市蒙圩镇柱强种养专业合作社 | A kind of young age Paris polyphylla underground stem tuber promotees strong cultural method |
| CN110226480A (en) * | 2019-07-17 | 2019-09-13 | 贵州百聚隆康中药材种植有限公司 | A kind of planting rhizoma polygonati method for culturing seedlings |
| CN110338018B (en) * | 2019-08-12 | 2021-12-17 | 腾冲谢家山金铁锁种植专业合作社 | Multi-bud paris polyphylla division cultivation method |
| CN113692934B (en) * | 2020-05-22 | 2022-12-23 | 中科农林科技有限公司 | Paeonia lactiflora breeding method |
| CN112056199A (en) * | 2020-09-14 | 2020-12-11 | 浙江省萧山棉麻研究所 | Seedling storage and breeding method for hemerocallis fulva |
| CN113994790B (en) * | 2021-11-09 | 2022-07-22 | 广西壮族自治区中国科学院广西植物研究所 | Method for promoting quick germination and seedling emergence of paris polyphylla rhizome buds |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09172817A (en) * | 1995-12-27 | 1997-07-08 | Yukijirushi Shubyo Kk | Mat-like flower seedling and its production |
| CN101142881A (en) * | 2007-10-18 | 2008-03-19 | 云南省农业科学院高山经济植物研究所 | Roots and stems section-cutting seedling-growing method for paris yunnanensis franch |
| CN101147441A (en) * | 2007-10-18 | 2008-03-26 | 云南省农业科学院高山经济植物研究所 | Seedlings growing method for promoting emergence rate of paris yunnanensis franch seeds |
| CN101248727A (en) * | 2008-04-07 | 2008-08-27 | 云南省农业科学院 | Artificial three-segment cultivating Paris Chinensis method |
| CN103444391A (en) * | 2013-07-31 | 2013-12-18 | 王小婵 | Cutting propagation and cultivation method for Paris polyphylla |
| CN104081911A (en) * | 2014-07-18 | 2014-10-08 | 云南省农业科学院药用植物研究所 | Method for shortening paris polyphylla seed germination period |
-
2014
- 2014-12-12 CN CN201410764239.6A patent/CN105723998B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09172817A (en) * | 1995-12-27 | 1997-07-08 | Yukijirushi Shubyo Kk | Mat-like flower seedling and its production |
| CN101142881A (en) * | 2007-10-18 | 2008-03-19 | 云南省农业科学院高山经济植物研究所 | Roots and stems section-cutting seedling-growing method for paris yunnanensis franch |
| CN101147441A (en) * | 2007-10-18 | 2008-03-26 | 云南省农业科学院高山经济植物研究所 | Seedlings growing method for promoting emergence rate of paris yunnanensis franch seeds |
| CN101248727A (en) * | 2008-04-07 | 2008-08-27 | 云南省农业科学院 | Artificial three-segment cultivating Paris Chinensis method |
| CN103444391A (en) * | 2013-07-31 | 2013-12-18 | 王小婵 | Cutting propagation and cultivation method for Paris polyphylla |
| CN104081911A (en) * | 2014-07-18 | 2014-10-08 | 云南省农业科学院药用植物研究所 | Method for shortening paris polyphylla seed germination period |
Non-Patent Citations (2)
| Title |
|---|
| 云南重楼种苗繁育技术;陈翠等;《中国现代中药》;20100228;第12卷(第02期);第23-24，28页 * |
| 甘孜州重楼高产栽培技术;崔宝禄;《四川农业科技》;20091231(第07期);第42页 * |
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