CN105684735B - Bottle plants the control bacterium method of disposal of Pleurotus eryngii industrial transfer room - Google Patents
Bottle plants the control bacterium method of disposal of Pleurotus eryngii industrial transfer room Download PDFInfo
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- CN105684735B CN105684735B CN201610081074.1A CN201610081074A CN105684735B CN 105684735 B CN105684735 B CN 105684735B CN 201610081074 A CN201610081074 A CN 201610081074A CN 105684735 B CN105684735 B CN 105684735B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
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Abstract
The present invention discloses the control bacterium method of disposal of a kind of bottle of cultivation Pleurotus eryngii industrial transfer room, belong to the control bacterium disinfection technology method field of Pleurotus eryngii industrial transfer room, comprising the following steps: including following operation: (1) transfer room air blowing control (2) transfer room Temperature and Humidity Control (3) must periodically sterilize to the night disinfection (4) of inoculation room air and machinery equipment to the hopper and tool of contact strain material before clean bodyguard suffocating sterilization (5) inoculation.The present invention by bottle plant Pleurotus eryngii be inoculated with many kinds of measures such as the processing of indoor high-efficient purification, humidity and temperature control, sterilization, sanitation and hygiene to before inoculation, seeded process, the comprehensive sterilizing after inoculation dispose, to reach to the comprehensively control polluted is inoculated with, to reduce the pollution rate that bottle plants Pleurotus eryngii to the greatest extent.
Description
Technical field
The present invention relates to the control bacterium disinfection technology fields of Pleurotus eryngii industrial transfer room, particularly relate to a kind of bottle of cultivation Pleurotus eryngii
The control bacterium method of disposal of batch production transfer room.
Background technique
China's edible mushroom total output, annual value of production increase year by year, are the maximum Edible Fungi countries of consumption in the whole world, account for the world year
The 70% of total output becomes the fifth-largest crop that China's agriculture field is only second to grain, oil, fruit, dish.Wherein, Pleurotus eryngii is a kind of
Large-scale meat agaric also known as pleurotus eryngii are that exploitation cultivation in recent years successfully integrates edible, medicinal, dietotherapy rare
Edible mushroom new varieties.
China is for edible fungus industrial production development is rapid, and batch production Pleurotus eryngii output in China's is in total yield at present
It increases sharply in amount, this is mainly the increase of production rate of bag cultivation Pleurotus eryngii 80% or more, and bag plants the quality and mushroom type of Pleurotus eryngii
1 class all at least poorer than the quality and mushroom type of bottle cultivation Pleurotus eryngii, due to by investment level, technical level, the limit of R & D Level
System, bottle plant Pleurotus eryngii industrial production and meet with bottleneck, easily cause living contaminants in the process, and a main factor is that bottleneck is big,
The area of culture medium and contacting external air is big when inoculation, and leakproofness does not have bag cultivation Pleurotus eryngii good, transfer room health, disinfection, water
Point, temperature, the control such as air-supply is not in place, inoculation is easily caused to pollute, there are 7 Jia Pingzai Pleurotus eryngii manufacturers in the country, due to dirt
Dye in place, has been closed down four without control, can be taken in the prior art from before inoculation, in inoculation, after inoculation there has been no a kind of
Measure is disposed by the control bacterium of the batch production transfer room of air-supply, temperature, humidity, disinfection, sterilization, monitoring etc. comprehensively control
Method.
Summary of the invention
The object of the present invention is to provide the control bacterium methods of disposal that a kind of bottle is planted Pleurotus eryngii industrial transfer room, by planting to bottle
Pleurotus eryngii is inoculated with many kinds of measures such as the processing of indoor high-efficient purification, humidity and temperature control, sterilization, sanitation and hygiene to inoculation
Joint disposal in advance, in thing, subsequent, come reach to inoculation pollution comprehensively control, thus to the greatest extent reduce bottle plant
The pollution rate of Pleurotus eryngii.
The present invention propose a kind of bottle cultivation Pleurotus eryngii industrial transfer room control bacterium method of disposal, which is characterized in that including with
Lower operation:
(1) transfer room air blowing control: transfer room use with just effect, it is middle effect, efficient three-stage filtration net wind cabinet blower and
The high-efficiency filtration systems of FFU blower, open middle-grade positive pressure wind when being inoculated with operation, the wind pressure of transfer room is that entire cleaning shop is highest
Wind pressure, and clean weekly, regularly replace three-stage filtration net；HEPA high efficiency particle air filter is equipped in the FFU blower, HEPA's
Filter screen reaches 99.99% for the particle effective percentage of 0.1 micron and 0.3 micron；Wind cabinet blower is loaded on transfer room top, FFU
Blower is in transfer room, and positioned at the lower section of high efficiency particulate air air outlet, the wind pressure of FFU blower upper space is in positive pressure shape
State avoids making the halfway air of disinfection outside transfer room from pulling in transfer room；
(2) transfer room Temperature and Humidity Control: ensure transfer room is maintained at 60% for humidity 24 hours hereinafter, using anion group
Dehumidifier carries out wet down, disinfection；During inoculation, the temperature of transfer room is controlled at 15-18 DEG C；
(3) night of inoculation room air and machinery equipment is sterilized: uses ozone and ultraviolet disinfection, every night ozonization 1-
2 times, 30 minutes or more every time, concentration 18-22ppm, transfer room kept sealing state when disinfection；Every night disinfection by ultraviolet light twice,
40-45 minute every time, ultraviolet lamp disembark device and conveyer belt distance no more than 1.5 meters, the ultraviolet lamp inside inoculation device is beaten the whole night
It opens and machine inside is sterilized；It is the period of evening ozone and disinfection by ultraviolet light, out-of-blast；Refrigeration machine is opened, and transfer room is controlled
Temperature is at 15-18 DEG C；The position of ozone air inlet pipe is located at the top of FFU blower；
(4) periodically must clean bodyguard suffocating sterilization: one month it is primary with must clean bodyguard fumigation is carried out to transfer room, it is stifling to put minute
Cloth is uniform, and fumigation time 4-8 hours；
(5) it sterilizes before being inoculated with to the hopper and tool of contact strain material: hopper and tool for preferably using flame sterilization
75% alcohol is cooperated to spray the burning sterilizing of its surface with 95% Alcohol Flame；For the tool of fire sterilizing should not be used with 75%
Alcohol sprinkling or wiping sterilizing.
Preferably, when being inoculated with operation, wind speed is 0.45 ± 0.9m/s, and every 1-2 hour surveys wind pressure and wind speed, examine per hour
Testing temperature and humidity；Every month overhauls wind cabinet blower, FFU blower, clears up the miscellaneous bits of filter screens at different levels.
Primary, every monthly repair, appearance obfuscation phenomenon or the timely replacement that do not work are wiped to ultraviolet lamp weekly.
Stifling point height in 1.5 ± 0.2m, must the dosage of clean bodyguard suffocating sterilization be that 5m is fumigated in a piece of A agent medicine+5mlB agent3-
10m3。
Hopper and tool sterilizing concrete operations to contact strain material are: with 95% Alcohol Flame to each digging kind tool
Cutter head calcination 4 seconds；For hopper and other tools, the mobile calcination of 95% cotton ball soaked in alcohol lighted is taken with long forceps sub-folder, meanwhile,
With the alcohol of mobile flame spraying 75% to its surface, covers Alcohol Flame thoroughly and contact maximally related worksheet with strain
Face；Rotation spray disinfectant is carried out to the strain bottleneck of each upper inoculation device with 75% alcohol.
Further include it is finished after cleaning and sterilizing to transfer room: empty seed bottle is removed inoculation room, hopper is dismantled
Cleaning of getting off is dried, and is wiped after being cleared up with residue and dust of the air gun to the dead angle of inoculation device, conveyer belt and transfer room wall
It wipes completely, waste residue is thoroughly swept out into transfer room；It is uniform to transfer room wall after the bottle outlet of transfer room and bottle inlet mouth are blocked
The ethanol for disinfection for spraying 75% carries out ground sterilization after being watered with lysol or " 84 " thimerosal.
Further include the Sterility testing to transfer room: 1-2 microbial sterility detection being done to transfer room weekly, is carried out in inoculation
By sterile petri dish in 1-2 hour, it is equably put into 6-8 point of transfer room, ware lid is opened placement and is covered after twenty minutes
Ware lid is simultaneously sealed, and is put into 30-32 DEG C of constant incubator and is detected after 2-3 days, each maximum allowable micro organism quantity of culture dish
2 should be no more than, if exceeded should search reason and exclude.
The beneficial effects of the present invention are: carrying out purifying and sterilizing to circulated air using high-efficiency filtration systems when daytime is inoculated with, just
Pressure wind is the pure air with pressure that dust and miscellaneous bacteria are not contained by just imitating, after middle effect, efficient and FFU blower fan filtering,
Inoculation is just not in pollute only under the environment of this cleaning, is inoculated under conditions of calm inoculation, and when inoculation is not achieved
Certain cleanliness is easy to that inoculation is caused to pollute.Anion group dehumidifier can play a dual role of dehumidifying, disinfection, positive pressure
Wind is that the synthesis for the wind pressure that FFU blower circulation is reinforced also is crossed in subenvironment back warp from the extraneous circulated air for entering transfer room,
Circulated air can play exsiccation to a certain extent, but when the ambient humidities such as ozone, ultraviolet disinfection and rainy season are big at night,
Main exsiccation is played with anion group's dehumidifier, steam is avoided to be adhered on FFU blower, damages FFU blower.Ozone and purple
Outer disinfection can only idle evening nobody when thorough disinfection is carried out to inoculation space, positive pressure wind and close is closed when ozonization
Envelope, can keep ozone concentration.Refrigeration machine is opened, and ozone not only can be evenly distributed in entire transfer room, but also can
With the Disinfection Effect of the holding ozone of long period.The wind pressure of transfer room is the highest wind pressure in entire cleaning shop, is guaranteed other
The air of clean area enters less than transfer room.The position of ozone air inlet pipe will generally be put into the top of FFU blower, in this way to entire
The ozone in room is more evenly distributed, and sterilization effect is more preferable.With must clean bodyguard fumigation is carried out to transfer room, miscellaneous bacteria pair in order to prevent
Variation caused by single-minded ozone and ultraviolet disinfection.1-2 microbial sterility detection is done to transfer room, carries out 1-2 in inoculation
In hour, this period not only can detecte to evening Disinfection Effect but also can detecte efficient and FFU blower filter effect.
In conclusion the determination of comprehensive use and various disinfections and dust removal method parameter by the above sterilization method, connects
The cleanliness of kind room reaches between hundred grades and thousand grades of the standard of transfer room clean level and microorganism detection sedimentation, i.e., by above-mentioned
Detection method, each maximum allowable micro organism quantity of culture dish are no more than 2.Bottle plant pollution rate by not use this technology it
Preceding 10 ‰ or so, drop to present 2 ‰ or so, are fallen below within controlled range value completely, daily the amount of 80,000 bottles of production, often
It can reduce pollution and scrap 640 bottles, and every bottle of production cost can reduce 576 yuan of cost allowance, every month at 0.9 yuan daily
It can reduce by 1.5 ten thousand yuan of loss within 26 days, while also avoid the negative effect for causing to pollute because seriously polluting to entire plant area,
It solves bottle and plants the high bottleneck of Pleurotus eryngii pollution rate, lay a good foundation for Pleurotus eryngii quality stability.
Specific embodiment
One the present embodiment of embodiment proposes the control bacterium method of disposal of a kind of bottle of cultivation Pleurotus eryngii industrial transfer room, including following
Operation:
(1) transfer room air blowing control: transfer room use with just effect, it is middle effect, efficient three-stage filtration net wind cabinet blower and
The high-efficiency filtration systems of FFU blower, open middle-grade positive pressure wind when being inoculated with operation, the wind pressure of transfer room is that entire cleaning shop is highest
Wind pressure, and clean weekly, regularly replace three-stage filtration net.HEPA high efficiency particle air filter is equipped in the FFU blower, HEPA's
Filter screen reaches 99.99% for the particle effective percentage of 0.1 micron and 0.3 micron；Wind cabinet blower is loaded on transfer room top, FFU
Blower is in transfer room, and positioned at the lower section of high efficiency particulate air air outlet, the wind pressure of FFU blower upper space is in positive pressure shape
State avoids making the halfway air of disinfection outside transfer room from pulling in transfer room.
(2) transfer room Temperature and Humidity Control: ensure transfer room is maintained at 60% for humidity 24 hours hereinafter, using anion group
Dehumidifier carries out wet down, disinfection；During inoculation, the temperature of transfer room is controlled at 15-18 DEG C；
(3) night of inoculation room air and machinery equipment is sterilized: uses ozone and ultraviolet disinfection, every night ozonization 1-
2 times, 30 minutes or more every time, concentration 18-22ppm, transfer room kept sealing state when disinfection；Every night disinfection by ultraviolet light twice,
40-45 minute every time, ultraviolet lamp disembark device and conveyer belt distance no more than 1.5 meters, the ultraviolet lamp inside inoculation device is beaten the whole night
It opens and machine inside is sterilized；It is the period of evening ozone and disinfection by ultraviolet light, out-of-blast；Refrigeration machine is opened, and transfer room is controlled
Temperature is at 15-18 DEG C.
(4) periodically must clean bodyguard suffocating sterilization: one month it is primary with must clean bodyguard fumigation is carried out to transfer room, it is stifling to put minute
Cloth is uniform, and fumigation time 4-8 hours.
(5) it sterilizes before being inoculated with to the hopper and tool of contact strain material: hopper and tool for preferably using flame sterilization
75% alcohol is cooperated to spray the burning sterilizing of its surface with 95% Alcohol Flame；For the tool of fire sterilizing should not be used with 75%
Alcohol sprinkling or wiping sterilizing.Concrete operations are: with 95% Alcohol Flame to each digging kind tool insert calcination 4 seconds；For
Hopper and other tools take the mobile calcination of 95% cotton ball soaked in alcohol lighted with long forceps sub-folder, meanwhile, with mobile flame spraying
75% alcohol covers Alcohol Flame thoroughly and contacts maximally related working surface with strain to its surface；With 75% alcohol
Rotation spray disinfectant is carried out to the seed bottle of each upper inoculation device.
(6) cleaning and sterilizing after finished to transfer room: empty seed bottle is removed inoculation room, hopper is disassembled
Cleaning is dried, and is wiped after being cleared up with residue and dust of the air gun to the dead angle of inoculation device, conveyer belt and transfer room wall
Only, waste residue is thoroughly swept out into transfer room；After the bottle outlet of transfer room and bottle inlet mouth are blocked, transfer room wall is uniformly sprayed
75% ethanol for disinfection carries out ground sterilization after being watered with lysol or " 84 " thimerosal.
(7) to the Sterility testing of transfer room: doing 1-2 microbial sterility detection to transfer room weekly, carry out 1-2 in inoculation
By sterile petri dish in a hour, it is equably put into 6-8 point of transfer room, ware lid is opened placement and covers ware after twenty minutes
It covers and seals, be put into 30-32 DEG C of constant incubator and detected after 2-3 days, each maximum allowable micro organism quantity of culture dish is answered
No more than 2, if exceeded should search reason and exclude.
When being inoculated with operation, wind speed is 0.45 ± 0.9m/s, and every 1-2 hour surveys wind pressure and wind speed, detect temperature per hour
And humidity；Every month overhauls wind cabinet blower, FFU blower, clears up the miscellaneous bits of filter screens at different levels.
The position of ozone air inlet pipe is located at the top of FFU blower, wipes primary, every monthly repair, appearance to ultraviolet lamp weekly
The phenomenon that shades or the timely replacement that do not work.
Stifling point height in 1.5 ± 0.2m, must the dosage of clean bodyguard suffocating sterilization be that 5m is fumigated in a piece of A agent medicine+5mlB agent3-
10m3。
FFU is english abbreviation, and full name is Fan Filter Units, and the Chinese meaning is " fan filter screen unit ".Definitely
Say it is a kind of self-powered modular end air flow device with filter efficacy.HEPA refers to that the Chinese meaning is efficiently empty
Air filter reaches the filter screen of HEPA standard, reaches 99.99%, HEPA net for the effective percentage of 0.1 micron and 0.3 micron
The characteristics of be that air can pass through, but tiny particle can not pass through.
Claims (7)
1. the control bacterium method of disposal of a kind of bottle of cultivation Pleurotus eryngii industrial transfer room, which is characterized in that including following operation:
(1) transfer room air blowing control: transfer room use with just effect, it is middle effect, efficient three-stage filtration net wind cabinet blower and FFU wind
The high-efficiency filtration systems of machine open middle-grade positive pressure wind when being inoculated with operation, the wind pressure of transfer room is the highest wind pressure in entire cleaning shop,
And it cleans weekly, regularly replace three-stage filtration net；HEPA high efficiency particle air filter, the filtering of HEPA are equipped in the FFU blower
Net reaches 99.99% for the particle effective percentage of 0.1 micron and 0.3 micron；Wind cabinet blower is loaded on transfer room top, FFU blower
In transfer room, positioned at the lower section of high efficiency particulate air air outlet, the wind pressure of FFU blower upper space is in barotropic state, keeps away
Exempt to make the halfway air of disinfection outside transfer room to pull in transfer room；
(2) transfer room Temperature and Humidity Control: ensure transfer room is maintained at 60% for humidity 24 hours hereinafter, dehumidifying using anion group
Machine carries out wet down, disinfection；During inoculation, the temperature of transfer room is controlled at 15-18 DEG C；
(3) to inoculation room air and machinery equipment night sterilize: use ozone and ultraviolet disinfection, ozonization 1-2 times every night,
30 minutes or more every time, concentration 18-22ppm, transfer room kept sealing state when disinfection；Every night disinfection by ultraviolet light twice, every time
40-45 minutes, the disembark distance of device and conveyer belt of ultraviolet lamp was no more than 1.5 meters, the ultraviolet lamp inside inoculation device, opened the whole night pair
Disinfection inside machine；It is the period of evening ozone and disinfection by ultraviolet light, out-of-blast；Refrigeration machine is opened, control inoculation room temperature
At 15-18 DEG C；The position of ozone air inlet pipe is located at the top of FFU blower；
(4) periodically must clean bodyguard suffocating sterilization: one month it is primary with must clean bodyguard fumigation is carried out to transfer room, fumigate point and be distributed
It is even, fumigation time 4-8 hours；
(5) it sterilizes before being inoculated with to the hopper and tool of contact strain material: the hopper and tool of preferably using flame sterilization is used
95% Alcohol Flame cooperates 75% alcohol to spray the burning sterilizing of its surface；For should not use fire sterilize tool with 75% wine
Essence sprinkling or wiping sterilizing.
2. the control bacterium method of disposal that bottle according to claim 1 plants Pleurotus eryngii industrial transfer room, which is characterized in that inoculation
When operation, wind speed is 0.45 ± 0.9m/s, and every 1-2 hour surveys wind pressure and wind speed, detect temperature and humidity per hour；Every month
Wind cabinet blower, FFU blower are overhauled, the miscellaneous bits of filter screens at different levels are cleared up.
3. the control bacterium method of disposal that bottle according to claim 1 plants Pleurotus eryngii industrial transfer room, which is characterized in that weekly
Primary to ultraviolet lamp wiping, there is obfuscation phenomenon or the timely replacement that do not work in every monthly repair.
4. the control bacterium method of disposal that bottle according to claim 1 plants Pleurotus eryngii industrial transfer room, which is characterized in that stifling
Point height in 1.5 ± 0.2m, must the dosage of clean bodyguard suffocating sterilization be that 5m is fumigated in a piece of A agent medicine+5mlB agent3-10m3。
5. the control bacterium method of disposal that bottle according to claim 1 plants Pleurotus eryngii industrial transfer room, which is characterized in that docking
The hopper and tool sterilizing concrete operations for touching strain material are: with 95% Alcohol Flame to each digging kind tool insert calcination 4 seconds；
For hopper and other tools, the mobile calcination of 95% cotton ball soaked in alcohol lighted is taken with long forceps sub-folder, meanwhile, with mobile flame
The alcohol of sprinkling 75% covers Alcohol Flame thoroughly and contacts maximally related working surface with strain to its surface；With 75%
Alcohol carries out rotation spray disinfectant to the strain bottleneck of each upper inoculation device.
6. the control bacterium method of disposal that bottle according to claim 1 plants Pleurotus eryngii industrial transfer room, which is characterized in that also wrap
Include it is finished after cleaning and sterilizing to transfer room: empty seed bottle is removed inoculation room, hopper is disassembled cleaning and is dried,
Wiped clean after being cleared up with residue and dust of the air gun to the dead angle of inoculation device, conveyer belt and transfer room wall, by waste residue
Thoroughly sweep out transfer room；After the bottle outlet of transfer room and bottle inlet mouth are blocked, 75% is uniformly sprayed to transfer room wall and is disappeared
Poisonous wine essence, carries out ground sterilization after being watered with lysol or " 84 " thimerosal.
7. the control bacterium method of disposal that bottle according to claim 1 plants Pleurotus eryngii industrial transfer room, which is characterized in that also wrap
It includes the Sterility testing to transfer room: 1-2 microbial sterility detection being done to transfer room weekly, is carried out in 1-2 hour in inoculation
By sterile petri dish, it is equably put into 6-8 point of transfer room, ware lid opening is placed and covers ware lid after twenty minutes and seals,
It being put into 30-32 DEG C of constant incubator and is detected after 2-3 days, each maximum allowable micro organism quantity of culture dish should be no more than 2,
If exceeded should search reason and exclude.
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| CN107616063A (en) * | 2017-10-21 | 2018-01-23 | 郴州市芝草农业科技开发有限公司 | Pleurotus eryngii bottle plants the comprehensive sterilization of factorial praluction inoculation |
| CN111567324A (en) * | 2020-06-03 | 2020-08-25 | 湖南省宇秀生物科技有限公司 | Method for sterilizing strain pipeline and spray head of online liquid inoculation machine by steam |
| CN111699926A (en) * | 2020-07-06 | 2020-09-25 | 湖南果秀食品有限公司 | Fungus bottle body for inoculating pleurotus eryngii with liquid seeds and comprehensive fungus bottle culture method |
| CN115250827A (en) * | 2022-08-24 | 2022-11-01 | 江苏菇本堂生物科技股份有限公司 | Method for comprehensively preventing and treating velvet antler mushroom industrial production pollution |
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