CN105684735A - Bacterium control handling method for bottled pleurotus eryngii factory-like inoculating room - Google Patents
Bacterium control handling method for bottled pleurotus eryngii factory-like inoculating room Download PDFInfo
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- CN105684735A CN105684735A CN201610081074.1A CN201610081074A CN105684735A CN 105684735 A CN105684735 A CN 105684735A CN 201610081074 A CN201610081074 A CN 201610081074A CN 105684735 A CN105684735 A CN 105684735A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
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Abstract
The invention discloses a bacterium control handling method for a bottled pleurotus eryngii factory-like inoculating room and belongs to the field of bacterium control and disinfection technical methods for pleurotus eryngii factory-like inoculating rooms. The method comprises the following steps that firstly, air feeding of the inoculating room is controlled; secondly, the temperature and the humidity of the inoculating room are controlled; thirdly, night disinfection on the air and machine equipment of the inoculating room is carried out; fourthly, fumigation and sterilization are carried out regularly with Bijieshi; fifthly, hoppers and tools in contact with strain materials are sterilized before inoculating. According to the method, due to the fact that comprehensive sterilization handling for the period before inoculating, the inoculating process and the period after inoculating is carried out through various measures such as efficient purification handling, humidity and temperature control, sterilization and disinfection and cleaning in the inoculating bottled pleurotus eryngii inoculating room, comprehensive control over inoculating contamination is achieved, and thus the contamination rate on bottled pleurotus eryngii is reduced to the maximum degree.
Description
Technical field
The present invention relates to the control bacterium disinfection technology field of Pleurotus eryngii industrial transfer room, particularly relate to a kind of bottle of control bacterium method of disposal planting Pleurotus eryngii industrial transfer room。
Background technology
China's edible fungi total output, annual value of production increase year by year, are the maximum Edible Fungi countries of consumption in the whole world, account for the 70% of world's gross annual output amount, become China's agriculture field be only second to grain, oil, really, the fifth-largest crop of dish。Wherein, Pleurotus eryngii is a kind of large-scale meat gill fungi, has another name called pleurotus eryngii, is that exploitation cultivation in recent years successfully integrates Rare edible fungus new varieties edible, medicinal, dietetic therapy。
Current China for, edible fungus industrial production development is rapid, and China's batch production Pleurotus eryngii volume of production increases sharply in total output, this is mainly bag and plants the increase of production rate of Pleurotus eryngii more than 80%, quality and the mushroom type of bag cultivation Pleurotus eryngii all at least differ from 1 class than the quality of bottle cultivation Pleurotus eryngii and mushroom type, due to by investment level, technical merit, the restriction of R & D Level, bottle is planted Pleurotus eryngii industrial production and is met with bottleneck, and very easily causing living contaminants, a main factor in process is that bottleneck is big, and during inoculation, culture medium is big with the area of contacting external air, and sealing does not have bag cultivation Pleurotus eryngii good, transfer room health, sterilization, moisture, temperature, air-supplies etc. control not in place, very easily causing inoculation to pollute, domestic have 7 Jia Pingzai Pleurotus eryngii manufacturers, does not have control to put in place owing to polluting, has closed down four, not yet has the one can before inoculation in prior art, in inoculation, take measures after inoculation, by blowing, temperature, humidity, sterilization, sterilization, the control bacterium method of disposal of the batch production transfer room of the aspect Comprehensive Control such as monitoring。
Summary of the invention
It is an object of the invention to provide a kind of bottle of control bacterium method of disposal planting Pleurotus eryngii industrial transfer room, by bottle planted the high-efficient purification in Pleurotus eryngii transfer room processing, the many kinds of measures such as humidity and temperature controls, sterilization, the sanitation and hygiene joint disposal in advance, in thing, afterwards to inoculating, reach the Comprehensive Control that inoculation is polluted, thus farthest reducing bottle to plant the pollution rate of Pleurotus eryngii。
The present invention proposes a kind of bottle of control bacterium method of disposal planting Pleurotus eryngii industrial transfer room, including following operation:
(1) transfer room air blowing control: transfer room adopts the high-efficiency filtration systems with first effect, middle effect, the wind cabinet blower fan of efficient three-stage filtration net and FFU blower fan, middle-grade malleation wind is opened during inoculation operation, the blast of transfer room is the blast that whole cleaning shop is the highest, and cleans weekly, regularly replaces three-stage filtration net。
(2) transfer room Temperature and Humidity Control: guarantee that the humidity of transfer room is maintained at less than 60% in 24 hours, uses anion group's dehumidifier to carry out wet down, sterilization；During inoculation, the temperature of transfer room controls at 15-18 DEG C；
(3) night of transfer room air and machinery equipment is sterilized: adopting ozone and ultraviolet disinfection, ozonization 1-2 time every night, each more than 30 minutes, concentration 18-22ppm, during sterilization, transfer room kept sealing state；Disinfection by ultraviolet light twice every night, each 40-45 minute, uviol lamp disembarked the distance of device and conveyer belt less than 1.5 meters, the uviol lamp inside inoculation device, opens the whole night and is sterilized in machine inside；Evening, ozone and time period of disinfection by ultraviolet light, out-of-blast；Refrigeration machine is opened, and controls transfer room temperature 15-18 DEG C。
(4) regularly must clean bodyguard suffocating sterilization: within one month, once with transfer room being carried out clean bodyguard fumigation, stifling point is evenly distributed, fumigation time 4-8 hour。
(5) to the hopper of contact strain material and instrument sterilizing before inoculation: for 75% ethanol being coordinated to spray its surface combustion sterilizing by the hopper of flame sterilization and the instrument Alcohol Flame of 95%；For the instrument of fire sterilizing should not be used to spray or wiping sterilizing with the ethanol of 75%。
Preferably, during inoculation operation, wind speed is 0.45 ± 0.9m/s, within every 1-2 hour, surveys blast and wind speed, detects temperature and humidity per hour；Wind cabinet blower fan, FFU blower fan are overhauled by every month, and the assorted bits of filter screens at different levels are cleared up。
Being provided with HEPA high efficiency particle air filter in described FFU, the drainage screen of HEPA reaches 99.99% for the microgranule effective percentage of 0.1 micron and 0.3 micron；Wind cabinet blower fan is loaded on above transfer room, and FFU blower fan is contained in transfer room, is positioned at the lower section of high efficiency particulate air air outlet, and the blast of FFU upper space is in barotropic state, it is to avoid the halfway air that makes to sterilize outside transfer room draws in transfer room。
The position of ozone air inlet pipe is located at the top of FFU, weekly to uviol lamp wiping once, every monthly repair, obfuscation phenomenon or the replacing in time that do not work occur。
Stifling some height at 1.5 ± 0.2m, must the consumption of clean bodyguard suffocating sterilization be the stifling 5m of a piece of A agent medicine+5mlB agent3-10m3。
To hopper and the instrument sterilizing concrete operations contacting strain material it is: the Alcohol Flame with 95% digs kind of tool insert calcination 4 seconds to each；For hopper and other instruments, the cotton ball soaked in alcohol taking light 95% with long forceps sub-folder moves calcination, meanwhile, with the ethanol of FLAME 75% of movement to its surface, makes Alcohol Flame thoroughly cover and contacts maximally related working surface with strain；The strain bottleneck of upper inoculation device every time is rotated spray disinfectant by the ethanol with 75%。
This method also include finished after to the cleaning of transfer room and sterilizing: empty seed bottle removal inoculation room, hopper is disassembled cleaning dry, wiped clean after clearing up with the air gun residue to the dead angle of inoculation device, conveyer belt and transfer room wall and dust, thoroughly sweeps out transfer room by waste residue；After the bottle outlet of transfer room and bottle inlet mouth are blocked, transfer room wall is uniformly sprayed the ethanol for disinfection of 75%, after being watered with lysol or " 84 " disinfectant solution, carries out ground sterilization。
This method also includes the Sterility testing to transfer room: transfer room does weekly 1-2 microbial sterility detection, carry out sterile petri dish in 1-2 hour in inoculation, it is put into 6-8 point of transfer room equably, ware lid is opened ware lid sealing on 20 minutes bonnets of placement, it is put in the constant incubator of 30-32 DEG C after 2-3 days and detects, the maximum allowable micro organism quantity of each culture dish less than 2, should should be searched reason if exceeded standard and get rid of。
The invention has the beneficial effects as follows: when daytime is inoculated, adopt high-efficiency filtration systems that circulated air is carried out purifying and sterilizing, malleation wind be by just effect, middle effect, efficiently and FFU filter after do not contain the pure air with pressure of dust and miscellaneous bacteria, inoculation does not only just have pollution under the environment of this cleaning, inoculate when calm inoculation, not reaching certain cleanliness factor during inoculation, as easy as rolling off a log causing inoculates pollution。Anion group's dehumidifier can play dehumidifying, sterilization dual function, malleation wind is the circulated air entering transfer room from the external world, also has the synthesis crossing the blast that FFU circulation is strengthened at subenvironment back warp, circulated air can play exsiccation to a certain extent, but when ozone, ultraviolet disinfection and rainy season etc., ambient humidity was big at night, play main exsiccation with anion group's dehumidifier, it is to avoid steam sticks on FFU, make FFU damage。Ozone and ultraviolet disinfection can only idle evening nobody time inoculation space is carried out thorough disinfection, close malleation wind during ozonization and seal, ozone concentration can be kept。Refrigeration machine open, be so possible not only to an ozone and be evenly distributed at whole transfer room, but also can the long period keep ozone Disinfection Effect。The blast of transfer room is the blast that whole cleaning shop is the highest, it is ensured that the air of other clean area enters less than transfer room。The position of ozone air inlet pipe to be generally put into the top of FFU, so the ozone in whole room is more evenly distributed, and sterilization effect is better。With clean bodyguard transfer room being carried out fumigation, for the variation preventing miscellaneous bacteria from single-minded ozone and ultraviolet disinfection being caused。Transfer room doing the detection of 1-2 microbial sterility, carries out in 1-2 hour in inoculation, this time period not only can detect Disinfection Effect but also can detect efficiently and the filter effect of FFU in evening。
In sum, comprehensive use through above sterilization method, and the determination of various sterilization and dust collection method parameter, the cleanliness factor of transfer room reaches between hundred grades and thousand grades of the standard of transfer room clean level and microorganism detection sedimentation, namely by above-mentioned detection method, the maximum allowable micro organism quantity of each culture dish is less than 2。Bottle plant pollution rate by do not adopt before this technology about 10 ‰, drop to present about 2 ‰, within falling below controlled range value completely, produce the amount of 80,000 bottles every day, pollution can be reduced every day and scrap 640 bottles, the production cost of every bottle is at 0.9 yuan, every day can reduce by the cost allowance of 576 yuan, the loss that 26 days every months can reduce 1.5 ten thousand yuan, also avoid simultaneously and cause the negative effect that whole plant area is polluted because of severe contamination, solve bottle and plant the bottleneck that Pleurotus eryngii pollution rate is high, lay a good foundation for Pleurotus eryngii quality stability。
Detailed description of the invention
Embodiment one the present embodiment proposes a kind of bottle of control bacterium method of disposal planting Pleurotus eryngii industrial transfer room, including following operation:
(1) transfer room air blowing control: transfer room adopts the high-efficiency filtration systems with first effect, middle effect, the wind cabinet blower fan of efficient three-stage filtration net and FFU blower fan, middle-grade malleation wind is opened during inoculation operation, the blast of transfer room is the blast that whole cleaning shop is the highest, and cleans weekly, regularly replaces three-stage filtration net。Being provided with HEPA high efficiency particle air filter in described FFU, the drainage screen of HEPA reaches 99.99% for the microgranule effective percentage of 0.1 micron and 0.3 micron；Wind cabinet blower fan is loaded on above transfer room, and FFU blower fan is contained in transfer room, is positioned at the lower section of high efficiency particulate air air outlet, and the blast of FFU upper space is in barotropic state, it is to avoid the halfway air that makes to sterilize outside transfer room draws in transfer room。
(2) transfer room Temperature and Humidity Control: guarantee that the humidity of transfer room is maintained at less than 60% in 24 hours, uses anion group's dehumidifier to carry out wet down, sterilization；During inoculation, the temperature of transfer room controls at 15-18 DEG C；
(3) night of transfer room air and machinery equipment is sterilized: adopting ozone and ultraviolet disinfection, ozonization 1-2 time every night, each more than 30 minutes, concentration 18-22ppm, during sterilization, transfer room kept sealing state；Disinfection by ultraviolet light twice every night, each 40-45 minute, uviol lamp disembarked the distance of device and conveyer belt less than 1.5 meters, the uviol lamp inside inoculation device, opens the whole night and is sterilized in machine inside；Evening, ozone and time period of disinfection by ultraviolet light, out-of-blast；Refrigeration machine is opened, and controls transfer room temperature 15-18 DEG C。
(4) regularly must clean bodyguard suffocating sterilization: within one month, once with transfer room being carried out clean bodyguard fumigation, stifling point is evenly distributed, fumigation time 4-8 hour。
(5) to the hopper of contact strain material and instrument sterilizing before inoculation: for 75% ethanol being coordinated to spray its surface combustion sterilizing by the hopper of flame sterilization and the instrument Alcohol Flame of 95%；For the instrument of fire sterilizing should not be used to spray or wiping sterilizing with the ethanol of 75%。Concrete operations are: the Alcohol Flame with 95% digs kind of tool insert calcination 4 seconds to each；For hopper and other instruments, the cotton ball soaked in alcohol taking light 95% with long forceps sub-folder moves calcination, meanwhile, with the ethanol of FLAME 75% of movement to its surface, makes Alcohol Flame thoroughly cover and contacts maximally related working surface with strain；The seed bottle of upper inoculation device every time is rotated spray disinfectant by the ethanol with 75%。
(6) to the cleaning of transfer room and sterilizing after finished: empty seed bottle removal inoculation room, hopper is disassembled cleaning dry, wiped clean after clearing up with the air gun residue to the dead angle of inoculation device, conveyer belt and transfer room wall and dust, thoroughly sweeps out transfer room by waste residue；After the bottle outlet of transfer room and bottle inlet mouth are blocked, transfer room wall is uniformly sprayed the ethanol for disinfection of 75%, after being watered with lysol or " 84 " disinfectant solution, carries out ground sterilization。
(7) Sterility testing to transfer room: weekly transfer room is done 1-2 microbial sterility detection, carry out sterile petri dish in 1-2 hour in inoculation, it is put into 6-8 point of transfer room equably, ware lid is opened ware lid sealing on 20 minutes bonnets of placement, it is put in the constant incubator of 30-32 DEG C after 2-3 days and detects, the maximum allowable micro organism quantity of each culture dish less than 2, should should be searched reason if exceeded standard and get rid of。
During inoculation operation, wind speed is 0.45 ± 0.9m/s, within every 1-2 hour, surveys blast and wind speed, detects temperature and humidity per hour；Wind cabinet blower fan, FFU blower fan are overhauled by every month, and the assorted bits of filter screens at different levels are cleared up。
The position of ozone air inlet pipe is located at the top of FFU, weekly to uviol lamp wiping once, every monthly repair, obfuscation phenomenon or the replacing in time that do not work occur。
Stifling some height at 1.5 ± 0.2m, must the consumption of clean bodyguard suffocating sterilization be the stifling 5m3-10m3 of a piece of A agent medicine+5mlB agent。
FFU is english abbreviation, and full name is FanFilterUnits, and the Chinese meaning is " fan filter screen unit "。Be exactly a kind of self-powered, there is the modular end air flow device of filter efficacy。HEPA refers to that the Chinese meaning is for high efficiency particle air filter, reaches the drainage screen of HEPA standard, and the effective percentage for 0.1 micron and 0.3 micron reaches 99.99%, and the feature of HEPA net is that air can pass through, but tiny microgranule cannot pass through。
Claims (8)
1. the control bacterium method of disposal of a bottle cultivation Pleurotus eryngii industrial transfer room, it is characterised in that include following operation:
(1) transfer room air blowing control: transfer room adopts the high-efficiency filtration systems with first effect, middle effect, the wind cabinet blower fan of efficient three-stage filtration net and FFU blower fan, middle-grade malleation wind is opened during inoculation operation, the blast of transfer room is the blast that whole cleaning shop is the highest, and cleans weekly, regularly replaces three-stage filtration net；
(2) transfer room Temperature and Humidity Control: guarantee that the humidity of transfer room is maintained at less than 60% in 24 hours, uses anion group's dehumidifier to carry out wet down, sterilization；During inoculation, the temperature of transfer room controls at 15-18 DEG C；
(3) night of transfer room air and machinery equipment is sterilized: adopting ozone and ultraviolet disinfection, ozonization 1-2 time every night, each more than 30 minutes, concentration 18-22ppm, during sterilization, transfer room kept sealing state；Disinfection by ultraviolet light twice every night, each 40-45 minute, uviol lamp disembarked the distance of device and conveyer belt less than 1.5 meters, the uviol lamp inside inoculation device, opens the whole night and is sterilized in machine inside；Evening, ozone and time period of disinfection by ultraviolet light, out-of-blast；Refrigeration machine is opened, and controls transfer room temperature 15-18 DEG C；
(4) regularly must clean bodyguard suffocating sterilization: within one month, once with transfer room being carried out clean bodyguard fumigation, stifling point is evenly distributed, fumigation time 4-8 hour；
(5) to the hopper of contact strain material and instrument sterilizing before inoculation: for 75% ethanol being coordinated to spray its surface combustion sterilizing by the hopper of flame sterilization and the instrument Alcohol Flame of 95%；For the instrument of fire sterilizing should not be used to spray or wiping sterilizing with the ethanol of 75%。
2. the control bacterium method of disposal of Pleurotus eryngii industrial transfer room planted by bottle according to claim 1, it is characterised in that during inoculation operation, wind speed is 0.45 ± 0.9m/s, within every 1-2 hour, surveys blast and wind speed, detects temperature and humidity per hour；Wind cabinet blower fan, FFU blower fan are overhauled by every month, and the assorted bits of filter screens at different levels are cleared up。
3. the control bacterium method of disposal of Pleurotus eryngii industrial transfer room planted by bottle according to claim 1, it is characterised in that being provided with HEPA high efficiency particle air filter in described FFU, the drainage screen of HEPA reaches 99.99% for the microgranule effective percentage of 0.1 micron and 0.3 micron；Wind cabinet blower fan is loaded on above transfer room, and FFU blower fan is contained in transfer room, is positioned at the lower section of high efficiency particulate air air outlet, and the blast of FFU upper space is in barotropic state, it is to avoid the halfway air that makes to sterilize outside transfer room draws in transfer room。
4. the control bacterium method of disposal of Pleurotus eryngii industrial transfer room planted by bottle according to claim 1, it is characterised in that the position of ozone air inlet pipe is located at the top of FFU；Weekly to uviol lamp wiping once, every monthly repair, obfuscation phenomenon or the replacing in time that do not work occur。
5. the control bacterium method of disposal of Pleurotus eryngii industrial transfer room planted by bottle according to claim 2, it is characterised in that stifling some height at 1.5 ± 0.2m, must the consumption of clean bodyguard suffocating sterilization be the stifling 5m of a piece of A agent medicine+5mlB agent3-10m3。
6. the control bacterium method of disposal of Pleurotus eryngii industrial transfer room planted by bottle according to claim 1, it is characterised in that to hopper and the instrument sterilizing concrete operations contacting strain material be: the Alcohol Flame with 95% digs kind of tool insert calcination 4 seconds to each；For hopper and other instruments, the cotton ball soaked in alcohol taking light 95% with long forceps sub-folder moves calcination, meanwhile, with the ethanol of FLAME 75% of movement to its surface, makes Alcohol Flame thoroughly cover and contacts maximally related working surface with strain；The strain bottleneck of upper inoculation device every time is rotated spray disinfectant by the ethanol with 75%。
7. the control bacterium method of disposal of Pleurotus eryngii industrial transfer room planted by bottle according to claim 1, it is characterized in that, also include finished after to the cleaning of transfer room and sterilizing: empty seed bottle removal inoculation room, hopper is disassembled cleaning dry, wiped clean after clearing up with the air gun residue to the dead angle of inoculation device, conveyer belt and transfer room wall and dust, thoroughly sweeps out transfer room by waste residue；After the bottle outlet of transfer room and bottle inlet mouth are blocked, transfer room wall is uniformly sprayed the ethanol for disinfection of 75%, after being watered with lysol or " 84 " disinfectant solution, carries out ground sterilization。
8. the control bacterium method of disposal of Pleurotus eryngii industrial transfer room planted by bottle according to claim 1, it is characterized in that, also include the Sterility testing to transfer room: weekly transfer room is done 1-2 microbial sterility detection, carry out sterile petri dish in 1-2 hour in inoculation, it is put into 6-8 point of transfer room equably, ware lid is opened ware lid sealing on 20 minutes bonnets of placement, it is put in the constant incubator of 30-32 DEG C after 2-3 days and detects, the maximum allowable micro organism quantity of each culture dish less than 2, should should be searched reason if exceeded standard and get rid of。
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Cited By (4)
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| CN107616063A (en) * | 2017-10-21 | 2018-01-23 | 郴州市芝草农业科技开发有限公司 | Pleurotus eryngii bottle plants the comprehensive sterilization of factorial praluction inoculation |
| CN111567324A (en) * | 2020-06-03 | 2020-08-25 | 湖南省宇秀生物科技有限公司 | Method for sterilizing strain pipeline and spray head of online liquid inoculation machine by steam |
| CN111699926A (en) * | 2020-07-06 | 2020-09-25 | 湖南果秀食品有限公司 | Fungus bottle body for inoculating pleurotus eryngii with liquid seeds and comprehensive fungus bottle culture method |
| CN115250827A (en) * | 2022-08-24 | 2022-11-01 | 江苏菇本堂生物科技股份有限公司 | Method for comprehensively preventing and treating velvet antler mushroom industrial production pollution |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111567324A (en) * | 2020-06-03 | 2020-08-25 | 湖南省宇秀生物科技有限公司 | Method for sterilizing strain pipeline and spray head of online liquid inoculation machine by steam |
| CN111699926A (en) * | 2020-07-06 | 2020-09-25 | 湖南果秀食品有限公司 | Fungus bottle body for inoculating pleurotus eryngii with liquid seeds and comprehensive fungus bottle culture method |
| CN115250827A (en) * | 2022-08-24 | 2022-11-01 | 江苏菇本堂生物科技股份有限公司 | Method for comprehensively preventing and treating velvet antler mushroom industrial production pollution |
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