CN107616063A - Pleurotus eryngii bottle plants the comprehensive sterilization of factorial praluction inoculation - Google Patents
Pleurotus eryngii bottle plants the comprehensive sterilization of factorial praluction inoculation Download PDFInfo
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- CN107616063A CN107616063A CN201710988339.0A CN201710988339A CN107616063A CN 107616063 A CN107616063 A CN 107616063A CN 201710988339 A CN201710988339 A CN 201710988339A CN 107616063 A CN107616063 A CN 107616063A
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Abstract
The present invention relates to a kind of pleurotus eryngii bottle to plant the comprehensive sterilization of factorial praluction inoculation, comprises the following steps：1）The transformation of transfer room filtration system：2）The control of transfer room humidity and temperature；3）It is inoculated with the ozone and ultraviolet disinfection of room air and machinery equipment；4）Disinfection agent of chlorine dioxide suffocating sterilization in transfer room：5）75% alcohol disinfecting and the sterilizing of 95% Alcohol Flame；6）Sanitation and hygiene after transfer room is finished：7）Transfer room Sterility testing：The present invention uses by the comprehensive of above sterilization method, and various sterilizations and the determination of dust removal method parameter, the pollution rate that bottle is planted is dropped to 2 ‰ or so by 10 ‰ or so before no optimization, is fallen below completely within controlled range value；Solve bottle and plant the high bottleneck of pleurotus eryngii pollution rate, laid a good foundation for pleurotus eryngii quality stability.
Description
Technical field
The present invention relates to the control bacterium disinfection technology field of Pleurotus eryngii industrial transfer room, particularly relates to a kind of pleurotus eryngii bottle and plants
The comprehensive sterilization of factorial praluction inoculation.
Background technology
China's edible mushroom total output, annual value of production increase year by year, are global maximum Edible Fungi countries of consumption, account for world's year
The 70% of total output, turn into the fifth-largest crop that China's agriculture field is only second to grain, oil, fruit, dish.
Wherein, pleurotus eryngii is a kind of large-scale meat agaric, also known as pleurotus eryngii, is the successfully collection food of exploitation cultivation in recent years
With, medicinal, dietotherapy in the Rare edible fungus new varieties of one.Pleurotus eryngii is nutritious, rich in protein, carbohydrate, more
The mineral matter such as sugared, vitamin and calcium, magnesium, copper, zinc, can improve immune function of human body, have anticancer, reducing blood lipid, profit to human body
Stomach and beauty etc. act on.Because it has the characteristics that nutritious, in good taste, shelf life is long, easy factory culture, in recent years
Carry out the popular welcome by domestic and international consumer and investor, there is good development potentiality.
China is for edible fungus industrial production development is rapid, and batch production pleurotus eryngii output in China's is in total yield at present
Increased sharply in amount, this be mainly the increase of production rate of plastic bag cultivation pleurotus eryngii more than 80%, the quality and mushroom type of plastic bag cultivation pleurotus eryngii are all
Than the quality and at least poor 1 class of mushroom type that bottle plants pleurotus eryngii, under degree of the plastic bag cultivation pleurotus eryngii yield close to saturation, market pair
The pleurotus eryngii demand of high-quality is also quickly increasing, due to being limited by investment level, technical merit, R ＆ D Level, batch production
Pleurotus eryngii bottle plants production and encounters bottleneck in process of production, and bottle, which is planted, easily causes miscellaneous bacteria dirty in Pleurotus eryngii industrial production process
Dye, a main factor is that bottleneck is big, and the area of culture medium and contacting external air is big during inoculation, and sealing does not have plastic bag cultivation apricot Bao
Mushroom is good, and the control such as transfer room health, sterilization, moisture, temperature, efficient is not in place, easily causes inoculation to pollute.It is same in order to avoid walking
The road of sample, the synthesis sterilization of pleurotus eryngii transfer room is planted to industrial bottle and has carried out beneficial exploration.
22 days 06 month, Chinese invention patent application publication number CN105684735A 2016, disclose a kind of bottle and plant apricot Bao
The control bacterium method of disposal of mushroom industrialized transfer room, including following operation：(1) transfer room air blowing control, (2) inoculation room temperature humidity control
System, (3) to inoculation room air and machinery equipment night sterilize, (4) periodically must clean bodyguard suffocating sterilization, (5) inoculation before dock
Touch hopper and the instrument sterilizing of strain material.The invention by bottle plant pleurotus eryngii transfer room in high-efficient purification handle, humidity and
The many kinds of measures such as temperature control, sterilization, sanitation and hygiene to before inoculation, seeded process, the comprehensive sterilizing disposal after inoculation, come
Reach the Comprehensive Control to inoculation pollution, so as to farthest reduce the pollution rate that bottle plants pleurotus eryngii.
The content of the invention
In view of under above-mentioned background, the present invention proposes that a kind of pleurotus eryngii bottle plants the comprehensive sterilization of factorial praluction inoculation, is
On the basis of CN105684735A, transfer room filtration system has been transformed, has been determined that bottle is planted in pleurotus eryngii transfer room by experiment,
Efficient purified treatment, air humidity and temperature, ozone, ultraviolet disinfection sterilization, Alcohol Flame sterilization, sanitation and hygiene knot
Close 75% alcohol, lysol, " 84 " thimerosal, must the various design parameters such as clean bodyguard fumigation, to reach to inoculation pollution
Comprehensive Control, so as to farthest reduce the pollution rate that bottle plants pleurotus eryngii, reach and the Contamination rate control of bottle cultivation pleurotus eryngii is existed
In zone of reasonableness.
Based on above-mentioned purpose, a kind of pleurotus eryngii bottle provided by the invention plants the comprehensive sterilization of factorial praluction inoculation, specifically
Comprise the following steps：
1）The transformation of transfer room filtration system：
（1）Increase one-level electrostatic precipitator before air cleaner；
（2）The blower fan inside elementary, middle and high wind cabinet must be first driven before inoculation production is started, opens electrostatic precipitator, then open
The blower fan of blower fan filtering unit, in process of production, it is necessary to assure pass through electrostatic precipitator into the wind inside transfer room, just,
Middle and high blower fan filtering unit；The air quantity of blower fan filtering unit is transferred to middle amount class, below every group of blower fan filtering unit, does
Upper red silk ribbon, during work, inspection personnel checks blast and wind speed each hour, during ensureing inoculation, transfer room one
Directly it is in barotropic state；
2）The control of transfer room humidity and temperature：
（1）The humidity one day of transfer room 24 hours, less than 60% is maintained at negative oxygen ion dehumidifier；
（2）During inoculation, for the temperature control of transfer room at 15-18 DEG C, transfer room will hang a mercurial thermometer sum temperature display
Degree meter；
（3）During inoculation, each hour does a comprehensive inspection and record to temperature and humidity；
3）It is inoculated with the ozone and ultraviolet disinfection of room air and machinery equipment:
（1）Pleurotus eryngii bottle planted the time of the ozonization of transfer room more than 30 minutes, and concentration is in 18-22ppm;Sterilize every night
Number is 1-2 times, the special Ozone Monitor of Concentration Testing；
（2）Uviol lamp, set time for twice, opening every time every night at 40 minutes, uviol lamp disembark device and conveyer belt distance not
More than 1.5 meters；
（3）Air-conditioning must be opened temperature range of the control in work at night；
（4）The period of ozone and disinfection by ultraviolet light at night, it is impossible to carry out mending fresh air and open cycle wind；
（5）Position on ozone air inlet pipe will typically be put into the top of blower fan filtering unit；
4）Disinfection agent of chlorine dioxide suffocating sterilization in transfer room：
In order to prevent miscellaneous bacteria to variation caused by single-minded ozone and ultraviolet disinfection, periodically with disinfection agent of chlorine dioxide to transfer room
Fumigation is carried out, dosage is calculated according to spatial volume；5m3-10m is fumigated in a piece of A agent medicine+5ml, B agent3；Stifling point distribution
Uniformly, highly it is advisable in 1.5m, fumigation time 4-8 hours；
5）75% alcohol disinfecting and the sterilizing of 95% Alcohol Flame：
（1）The hopper and instrument that contact strain material sterilized with 95% Alcohol Flame before inoculation, concrete operations
It is the flame lighted with 95% cotton ball soaked in alcohol, calcination 4 seconds is carried out to each kind of tool insert that digs；Correlation is contacted with strain
Instrument carries out flame sterilization；With 75% alcohol disinfecting plastics should not use fire sterilizing instrument, these processing after the completion of, with
Sterilized hopper is attached to above inoculation device by the hand of sterile gloves；
（2）For the seed bottle of each upper inoculation device, rotation sprinkling will be carried out with alcohol watering can to bottle mouth position with 75% alcohol
Sterilization；
6）Sanitation and hygiene after transfer room is finished：
（1）For when inoculation terminating every time, the seed bottle of sky being removed inoculation room, hopper is disassembled and uses running water
Clean up, be put into purge chamber and dry, wait inoculation room thoroughly to sweep health and place into transfer room；
（2）The dead angle of inoculation device and inoculation room is cleared up with air gun, waste material is swept out transfer room with besom and refuse hopper,
Wanting the transfer room that sweeps out compared with limits, process above strain waste residue will be repeated 3-5 times；
（3）For adhering to bacteria residue and dust on inoculation device and conveyer belt, wiped with the steel wire lump for speckling with clear water, after wiping
It is exactly again finally machine and biography with clean towel with the dirt thoroughly wiped containing cleaning agent towel on machine and conveyer belt
Send band to wipe clean, some hidden residues on transfer room wall, machine, conveyer belt are then blown to transfer room on the ground with air gun
Face, and sweep out transfer room；
（4）Wall is wiped with the towel with detergent, then with clean towel wall wiped clean, last wall
Wall uniformly sprays 75% ethanol for disinfection；
（5）With ground can be dragged a clean with the long mop of disassembly, cleaning, finally replaced again with lysol, " 84 " thimerosal
Use, ground sterilization is carried out to inoculation room floor；
（6）Inoculation hopper instrument after cleaning is dried is put into, above the shelf for putting strain frame, for transfer room bottle outlet and enter
Bottle mouth position, blocked with the polypropylene or stainless steel plate mutually agreed with oral area；
7）Transfer room Sterility testing：
（1）1-2 microbial sterility detection is done to transfer room weekly, sees the sterilization of transfer room, the effect of filter, filtering dress
The normal operation and the clean level of health put；
（2）The culture dish crossed with making and carrying out Sterility testing, uniformly it is put into 6-8 point of transfer room, the position each put
0.5 meter or so below FFU of position is put, typically carries out proceeding by placement in 1-2 hour in inoculation, ware after placing
Lid is opened, and is placed 20 minutes, is covered ware lid to the time, seal up culture dish with sealed membrane, prevent miscellaneous bacteria from entering in transfer process；
（3）Culture dish after detection is sealed, is put into 30-32 DEG C of constant incubator 2-3 days, observes the number of mould and bacterium
Amount, and contrasted with blank culture dish；
（4）The cleanliness factor of transfer room is typically between hundred grades and thousand grades, so the maximum allowable micro organism quantity of each culture dish should
No more than 2.
The present invention uses by the comprehensive of above sterilization method, and various sterilizations and the determination of dust removal method parameter, makes bottle
The pollution rate of cultivation drops to present 2 ‰ or so by 10 ‰ or so before no optimization, fall below completely controlled range value with
Interior, the amount of 80,000 bottles of production, can reduce pollution and scrap 640 bottles, every bottle of production cost can subtract daily at 0.9 yuan daily daily
Few 576 yuan of cost allowance, can reduce by 1.5 ten thousand yuan of loss 26 days every months, at the same also avoid because serious pollution and caused by
Negative effect to the pollution of whole plant area, solve bottle and plant the high bottleneck of pleurotus eryngii pollution rate, established for pleurotus eryngii quality stability
Basis is determined.
Sterilization method of the present invention, the pollution rate in bottle cultivation pleurotus eryngii seeded process can be effectively reduced, reduces life
The loss of cost is produced, the stability of production is added, alleviates plant area's pollution level, is carried for the stabilization of company, consecutive production
Sound assurance is supplied.
Embodiment
The present invention is described in detail with embodiment below.
1）The replacing of transfer room high-efficiency filtration systems, use, maintenance criterion：
（1）Scavenging air filter, replacing construction just imitate cleaning in one week twice, change once within one month；The cleaning one in one week of middle effect
It is secondary, change once within three months；Changed once within individual month efficiently per 4-6；Blower fan filtering unit is changed once for 1 year；
2）The blower fan inside elementary, middle and high wind cabinet must be first driven before inoculation production is started, then opens the wind of blower fan filtering unit
Machine, in process of production, it is necessary to assure pass through elementary, middle and high blower fan filtering unit into the wind inside transfer room；Blower fan filtering machine
The air quantity of group is transferred to middle amount class, below every group of blower fan filtering unit, does red silk ribbon, during work, inspection personnel
Each hour checks blast and wind speed, and during ensureing inoculation, transfer room is constantly in barotropic state；
（3）Maintaining, every month are detected to the belt of wind cabinet blower fan, the blower fan of also blower fan filtering unit, find pine
The on-call maintenance of relaxation, there is the timely of cracking to be sealed with glass cement blower fan filtering unit seam crossing, in filter screen gap below FFU
There is wood chip and cleared up in face；
2）The control of transfer room humidity and temperature：
（1）General 24 hours one day of the humidity of transfer room, is maintained at less than 60% with negative oxygen ion dehumidifier, plays wet down, disappears
The effect of poison；
（2）During inoculation, for the temperature control of transfer room at 15-18 DEG C, transfer room will hang a mercurial thermometer sum temperature display
Degree meter, guarantee be seeded in it is accurate rationally in a low temperature of carry out；
（3）During inoculation, each hour does a comprehensive inspection and record to temperature and humidity；
3）It is inoculated with the ozone and ultraviolet disinfection of room air and machinery equipment:
（1）Pleurotus eryngii bottle planted the time of the ozonization of transfer room more than 30 minutes, and concentration is in 18-22ppm;Sterilize every night
Number is 1-2 times, the special Ozone Monitor of Concentration Testing, in the case where space sealing is bad, to do a little baffle plates handles and enter
Material mouth and discharging opening are stifled tight with polypropylene plastics flitch or stainless steel shoe；
（2）Uviol lamp, set time for twice, opening every time every night at 40 minutes, uviol lamp disembark device and conveyer belt distance not
More than 1.5 meters, the uviol lamp inside inoculation device is general to open the whole night at night, being carried out disinfection inside machine, uviol lamp one
As phenomenon or do not worked and will change in time if there is obfuscation in one month, uviol lamp is wiped once weekly；
（3）Air-conditioning must be opened temperature range of the control in work at night, so not only ozone can be entirely being connect
Kind room is evenly distributed, but also can be with the Disinfection Effect of the holding ozone of long period；
（4）The period of ozone and disinfection by ultraviolet light at night, it is impossible to carry out mending fresh air and open cycle wind, so can just play pair
Article surface plays good disinfective action；
（5）Position on ozone air inlet pipe will typically be put into the top of blower fan filtering unit, the so ozone to whole room
It is more evenly distributed, sterilization effect is more preferable；
4）Must clean bodyguard board disinfection agent of chlorine dioxide suffocating sterilization in transfer room：
In order to prevent miscellaneous bacteria to being made a variation caused by single-minded ozone and ultraviolet disinfection, periodically（One month once）Disappeared with chlorine dioxide
Toxic agent carries out fumigation to transfer room, and dosage is calculated according to spatial volume.5m is fumigated in a piece of A agent medicine+5mlB agent3-10m3。
Stifling point is evenly distributed, and is highly advisable in 1.5m, fumigation time 4-8 hours.The ratio of 5mlB agent is poured into generally according to a piece of medicine
Calculate, B agent is poured into the plastic measuring glass in open nonmetallic vessel or in packaging, put well by the position code of stifling point, then throw
Enter A agent, launched by order from the inside to surface, withdraw scene after dispensing immediately；
5）75% alcohol disinfecting and the sterilizing of 95% Alcohol Flame：
（1）The hopper and instrument that contact strain material sterilized with 95% Alcohol Flame before inoculation, concrete operations
It is the flame lighted with 95% cotton ball soaked in alcohol, calcination 4 seconds is carried out to each kind of tool insert that digs；And strain related to hopper etc.
The instrument of contact carries out flame sterilization（Concrete operations, 95% cotton ball soaked in alcohol is taken with long forceps sub-folder, is lighted with lighter, then basis
The sequencing of the relevant instrument contacted with strain, one side movable flame cotton ball soaked in alcohol, while 75% alcohol is sprayed with watering can,
The inoculation hopper surface with flame cotton ball soaked in alcohol is sprayed onto, Alcohol Flame is thoroughly covered and maximally related instrument is contacted with strain
Surface, it is thoroughly sterilized, the instrument of fire sterilizing should not be used with 75% alcohol disinfecting plastics etc., after the completion of these processing, with wearing
Sterilized hopper is attached to above inoculation device by the hand for having sterile gloves；
（2）For the seed bottle of each upper inoculation device, rotation sprinkling will be carried out with alcohol watering can to bottle mouth position with 75% alcohol
Sterilization；
6）Sanitation and hygiene after transfer room is finished：
（1）For when inoculation terminating every time, the seed bottle of sky being removed inoculation room, hopper is disassembled and uses running water
Clean up, be put into purge chamber and dry, wait inoculation room thoroughly to sweep health and place into transfer room；
（2）The dead angle of inoculation device and inoculation room is cleared up with air gun, waste material is swept out transfer room with besom and refuse hopper,
Wanting the transfer room that sweeps out compared with limits, process above strain waste residue will be repeated 3-5 times；
（3）For adhering to bacteria residue and dust on inoculation device and conveyer belt, wiped with the steel wire lump for speckling with clear water, after wiping
It is exactly again finally machine and biography with clean towel with the dirt thoroughly wiped containing cleaning agent towel on machine and conveyer belt
Send band to wipe clean, some hidden residues on transfer room wall, machine, conveyer belt are then blown to transfer room on the ground with air gun
Face, and sweep out transfer room；
（4）Wall is wiped with the towel with detergent, then with clean towel wall wiped clean, last wall
Wall uniformly sprays 75% ethanol for disinfection；
（5）With ground can be dragged a clean with the long mop of disassembly, cleaning, finally replaced again with lysol, " 84 " thimerosal
Use, ground sterilization is carried out to inoculation room floor；
（6）Inoculation hopper instrument after cleaning is dried is put into, above the shelf for putting strain frame, for transfer room bottle outlet and enter
Bottle mouth position, blocked with the polypropylene or stainless steel plate mutually agreed with oral area；
7）Transfer room Sterility testing：
（1）1-2 microbial sterility detection is done to transfer room weekly, sees the sterilization of transfer room, the effect of filter, filtering dress
The normal operation and the clean level of health put；
（2）The culture dish crossed with making and carrying out Sterility testing, uniformly it is put into 6-8 point of transfer room, the position each put
0.5 meter or so below FFU of position is put, typically carries out proceeding by placement in 1-2 hour in inoculation, ware after placing
Lid is opened, and is placed 20 minutes, is covered ware lid to the time, seal up culture dish with sealed membrane, prevent miscellaneous bacteria from entering in transfer process；
（3）Culture dish after detection is sealed, is put into 30-32 DEG C of constant incubator 2-3 days, observes the number of mould and bacterium
Amount, and contrasted with blank culture dish；
（4）The standard settled about transfer room clean level and microorganism detection；
| Cleanliness class | Grit maximum allowable number/m3 >=0.5 μm | Grit maximum allowable number/m3 >=5 μm | Microorganism maximum allowable number flcating germ/m3 | Microorganism maximum allowable number settling bacteria/ware |
| 100 | 3,500 | 0 | 5 | 1 |
| 110,000 | 350,000 | 2,000 | 100 | 3 |
| 100,000 | 3,500,000 | 20,000 | 500 | 10 |
| 300,000 | 10,500,000 | 60,000 | - | 15 |
| 100 | 3,500 | 0 | 5 | 1 |
；
（5）The cleanliness factor of our transfer rooms is typically between hundred grades and thousand grades, so the maximum allowable microbe quantity of each culture dish
Amount should be no more than 2, and the reason for corresponding is had to look for if there is exceeded.
Claims (1)
1. a kind of pleurotus eryngii bottle provided by the invention plants the comprehensive sterilization of factorial praluction inoculation, following steps are specifically included：
1）The transformation of transfer room filtration system：
（1）Increase one-level electrostatic precipitator before air cleaner；
（2）The blower fan inside elementary, middle and high wind cabinet must be first driven before inoculation production is started, opens electrostatic precipitator, then open
The blower fan of blower fan filtering unit, in process of production, it is necessary to assure pass through electrostatic precipitator into the wind inside transfer room, just,
Middle and high blower fan filtering unit；The air quantity of blower fan filtering unit is transferred to middle amount class, below every group of blower fan filtering unit, does
Upper red silk ribbon, during work, inspection personnel checks blast and wind speed each hour, during ensureing inoculation, transfer room one
Directly it is in barotropic state；
2）The control of transfer room humidity and temperature：
（1）The humidity one day of transfer room 24 hours, less than 60% is maintained at negative oxygen ion dehumidifier；
（2）During inoculation, for the temperature control of transfer room at 15-18 DEG C, transfer room will hang a mercurial thermometer sum temperature display
Degree meter；
（3）During inoculation, each hour does a comprehensive inspection and record to temperature and humidity；
3）It is inoculated with the ozone and ultraviolet disinfection of room air and machinery equipment:
（1）Pleurotus eryngii bottle planted the time of the ozonization of transfer room more than 30 minutes, and concentration is in 18-22ppm;Sterilize every night
Number is 1-2 times, the special Ozone Monitor of Concentration Testing；
（2）Uviol lamp, set time for twice, opening every time every night at 40 minutes, uviol lamp disembark device and conveyer belt distance not
More than 1.5 meters；
（3）Air-conditioning must be opened temperature range of the control in work at night；
（4）The period of ozone and disinfection by ultraviolet light at night, it is impossible to carry out mending fresh air and open cycle wind；
（5）Position on ozone air inlet pipe will typically be put into the top of blower fan filtering unit；
4）Disinfection agent of chlorine dioxide suffocating sterilization in transfer room：
In order to prevent miscellaneous bacteria to variation caused by single-minded ozone and ultraviolet disinfection, periodically with disinfection agent of chlorine dioxide to transfer room
Fumigation is carried out, dosage is calculated according to spatial volume；5m is fumigated in a piece of A agent medicine+5mlB agent3-10m3；Stifling point distribution is equal
It is even, highly it is advisable in 1.5m, fumigation time 4-8 hours；
5）75% alcohol disinfecting and the sterilizing of 95% Alcohol Flame：
（1）The hopper and instrument that contact strain material sterilized with 95% Alcohol Flame before inoculation, concrete operations
It is the flame lighted with 95% cotton ball soaked in alcohol, calcination 4 seconds is carried out to each kind of tool insert that digs；Correlation is contacted with strain
Instrument carries out flame sterilization；With 75% alcohol disinfecting plastics should not use fire sterilizing instrument, these processing after the completion of, with
Sterilized hopper is attached to above inoculation device by the hand of sterile gloves；
（2）For the seed bottle of each upper inoculation device, rotation sprinkling will be carried out with alcohol watering can to bottle mouth position with 75% alcohol
Sterilization；
6）Sanitation and hygiene after transfer room is finished：
（1）For when inoculation terminating every time, the seed bottle of sky being removed inoculation room, hopper is disassembled and uses running water
Clean up, be put into purge chamber and dry, wait inoculation room thoroughly to sweep health and place into transfer room；
（2）The dead angle of inoculation device and inoculation room is cleared up with air gun, waste material is swept out transfer room with besom and refuse hopper,
Wanting the transfer room that sweeps out compared with limits, process above strain waste residue will be repeated 3-5 times；
（3）For adhering to bacteria residue and dust on inoculation device and conveyer belt, wiped with the steel wire lump for speckling with clear water, after wiping
It is exactly again finally machine and biography with clean towel with the dirt thoroughly wiped containing cleaning agent towel on machine and conveyer belt
Send band to wipe clean, some hidden residues on transfer room wall, machine, conveyer belt are then blown to transfer room on the ground with air gun
Face, and sweep out transfer room；
（4）Wall is wiped with the towel with detergent, then with clean towel wall wiped clean, last wall
Wall uniformly sprays 75% ethanol for disinfection；
（5）With ground can be dragged a clean with the long mop of disassembly, cleaning, finally replaced again with lysol, " 84 " thimerosal
Use, ground sterilization is carried out to inoculation room floor；
（6）Inoculation hopper instrument after cleaning is dried is put into, above the shelf for putting strain frame, for transfer room bottle outlet and enter
Bottle mouth position, blocked with the polypropylene or stainless steel plate mutually agreed with oral area；
7）Transfer room Sterility testing：
（1）1-2 microbial sterility detection is done to transfer room weekly, sees the sterilization of transfer room, the effect of filter, filtering dress
The normal operation and the clean level of health put；
（2）The culture dish crossed with making and carrying out Sterility testing, uniformly it is put into 6-8 point of transfer room, the position each put
0.5 meter or so below FFU of position is put, typically carries out proceeding by placement in 1-2 hour in inoculation, ware after placing
Lid is opened, and is placed 20 minutes, is covered ware lid to the time, seal up culture dish with sealed membrane, prevent miscellaneous bacteria from entering in transfer process；
（3）Culture dish after detection is sealed, is put into 30-32 DEG C of constant incubator 2-3 days, observes the number of mould and bacterium
Amount, and contrasted with blank culture dish；
（4）The cleanliness factor of transfer room is typically between hundred grades and thousand grades, so the maximum allowable micro organism quantity of each culture dish should
No more than 2.
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| CN201710988339.0A CN107616063A (en) | 2017-10-21 | 2017-10-21 | Pleurotus eryngii bottle plants the comprehensive sterilization of factorial praluction inoculation |
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| CN201710988339.0A CN107616063A (en) | 2017-10-21 | 2017-10-21 | Pleurotus eryngii bottle plants the comprehensive sterilization of factorial praluction inoculation |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111567324A (en) * | 2020-06-03 | 2020-08-25 | 湖南省宇秀生物科技有限公司 | Method for sterilizing strain pipeline and spray head of online liquid inoculation machine by steam |
| CN112088720A (en) * | 2020-09-19 | 2020-12-18 | 灌南云农食用菌研究所(有限合伙) | Pleurotus eryngii liquid culture medium and pleurotus eryngii liquid culture production process |
| CN114377183A (en) * | 2021-12-26 | 2022-04-22 | 盐城聚德机械零部件有限公司 | Sterilization filtering method of air filter |
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| CN111567324A (en) * | 2020-06-03 | 2020-08-25 | 湖南省宇秀生物科技有限公司 | Method for sterilizing strain pipeline and spray head of online liquid inoculation machine by steam |
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| CN114377183A (en) * | 2021-12-26 | 2022-04-22 | 盐城聚德机械零部件有限公司 | Sterilization filtering method of air filter |
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