CN213214724U - Aseptic inoculation device of tissue culture seedling that dendrobium officinale used - Google Patents
Aseptic inoculation device of tissue culture seedling that dendrobium officinale used Download PDFInfo
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- CN213214724U CN213214724U CN202021691212.6U CN202021691212U CN213214724U CN 213214724 U CN213214724 U CN 213214724U CN 202021691212 U CN202021691212 U CN 202021691212U CN 213214724 U CN213214724 U CN 213214724U
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- fixedly connected
- inoculation
- aseptic
- aseptic inoculation
- tissue culture
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- 238000011081 inoculation Methods 0.000 title claims abstract description 80
- 241001076416 Dendrobium tosaense Species 0.000 title claims abstract description 20
- 238000000926 separation method Methods 0.000 claims abstract description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 230000002421 anti-septic effect Effects 0.000 claims description 7
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 8
- 235000015097 nutrients Nutrition 0.000 abstract description 8
- 239000007921 spray Substances 0.000 abstract description 4
- 238000005192 partition Methods 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 241001523681 Dendrobium Species 0.000 description 2
- 241000973497 Siphonognathus argyrophanes Species 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 210000003437 trachea Anatomy 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an aseptic inoculation device of tissue culture seedling that dendrobium officinale was used, including aseptic inoculation box, the interior diapire of aseptic inoculation box inlays and is equipped with the bearing, the interior anchor ring fixedly connected with dwang of bearing, the surface difference fixedly connected with bearing block and the separation curtain of dwang, the last fixed surface of aseptic inoculation box is connected with servo motor, servo motor's output and the upper end fixed connection of dwang, the inner wall fixedly connected with ultraviolet lamp and the flame spray nozzle of aseptic inoculation box. This aseptic inoculation device of tissue culture seedling that dendrobium officinale was used, through setting up the partition curtain, can be with the internal partition of aseptic inoculation case for placing district and inoculation district, through setting up ultraviolet lamp, can utilize ultraviolet lamp light to place the inside tissue culture seedling test tube of waiting to inoculate of district and nutrient solution and disinfect, through setting up the flame shower nozzle, can disinfect to the test tube that the inoculation finishes, prevent to cause the pollution of test tube joint seedling at the in-process of inoculation.
Description
Technical Field
The utility model relates to an aseptic inoculation technical field specifically is an aseptic inoculation device of tissue culture seedling that dendrobium officinale used.
Background
Orchid plants form the characteristic of relying on mycorrhiza survival and growth in a long-term evolution process, typical mycorrhizal plants are plants which need fungi to provide nutrition for root systems in the growth process, dendrobium officinale is a treasure plant of orchid dendrobium, at present, sterile seeding is used in the market to obtain test-tube plantlets, and the problem of dendrobium shortage and wild resource protection are solved through a tissue culture seedling rapid propagation technology.
However, in tissue culture propagation, there are still many problems, such as the need to ensure the sterility of the environment during the rapid propagation of tissue culture seedlings, and the need to ensure that the nutrient solution added to the test-tube seedlings is also sterile.
Therefore, an aseptic inoculation device for tissue culture seedlings for dendrobium officinale is needed.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an aseptic inoculation device of tissue culture seedling that dendrobium officinale was used to solve the problem that provides among the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a sterile inoculation device of tissue culture seedling that dendrobium officinale used, includes aseptic inoculation hood, the interior diapire of aseptic inoculation hood inlays and is equipped with the bearing, the interior anchor ring fixedly connected with dwang of bearing, the surface of dwang is fixedly connected with bearing block and separation curtain respectively, the lateral wall fixedly connected with bearing bar of bearing block, the last fixed surface of aseptic inoculation hood is connected with servo motor, servo motor's output and the upper end fixed connection of dwang, the interior back wall fixedly connected with ultraviolet lamp and the flame shower nozzle of aseptic inoculation hood, the air inlet has been seted up to the right side wall of aseptic inoculation hood, the surface cover of air inlet is equipped with the filter screen, the inside wall fixedly connected with fan housing of aseptic inoculation hood, the interior diapire fixedly connected with of aseptic inoculation hood is equipped with the disinfection cup of antiseptic solution, the air-out end of fan housing extends to the inside of antiseptic solution through the pipeline, the left wall of aseptic seed receiving box has seted up the exhaust vent, the inner wall fixedly connected with air exhauster of exhaust vent.
Preferably, the lower fixed surface of aseptic inoculation hood is connected with the supporting leg, the quantity of supporting leg is four, four the supporting leg is the lower surface that the rectangular array set up at aseptic inoculation hood.
Preferably, the number of the bearing rods is two, and the upper surfaces of the two bearing rods are fixedly connected with the object carrying plate.
Preferably, the number of the separation curtains is two, and the two separation curtains and the bearing rod are arranged in the sterile inoculation box in a cross-shaped structure.
Preferably, a gas tank is arranged below the sterile inoculation box, and the flame nozzle is communicated with the gas tank through a gas pipe.
Preferably, the lateral wall fixedly connected with foot control switch of supporting leg, air exhauster, servo motor and ultraviolet lamp all with the coupling of foot control switch.
Preferably, the front of the sterile inoculation box is provided with a sliding groove, a folding soft curtain is connected inside the sliding groove in a sliding manner, the surface of the folding soft curtain is provided with four operation holes, and the number of the operation holes is four.
Advantageous effects
The utility model provides an aseptic inoculation device of tissue culture seedling that dendrobium officinale used possesses following beneficial effect:
1. this aseptic inoculation device of group banks up seedling for dendrobii officmalis caulis, separate the curtain through setting up, can be with the internal partitioning of aseptic inoculation case for placing district and inoculation district, through setting up ultraviolet lamp, can utilize ultraviolet lamp light to disinfect to placing the inside group of waiting to inoculate seedling test tube and nutrient solution of distinguishing, through setting up flame shower nozzle, keep the test tube mouth during the inoculation, bottleneck or culture dish crack department do not leave the aseptic district of flame, prevent to cause the pollution of test tube connector seedling at the in-process of inoculation, through setting up the air exhauster, disinfection cup and antiseptic solution, can disinfect and filter the air that outside air got into in the box, reached and prevented that outside bacterium from getting into the inside sterile environment's of box purpose.
2. This aseptic inoculation device of tissue culture seedling that dendrobium officinale used through setting up four handle holes, can two people operate simultaneously, through setting up servo motor and carrying the thing dish, can realize automatic commentaries on classics material under the condition of carrying out space separation and disinfection, when carrying out pipelined inoculation, can guarantee the aseptic nature of inoculation district environment.
Drawings
FIG. 1 is a schematic view of a front cross-sectional structure of the present invention;
FIG. 2 is a schematic front view of the present invention;
fig. 3 is the side-cut structure schematic diagram of the utility model.
In the figure: 1 sterile inoculation box, 2 rotating rods, 3 separation curtains, 4 servo motors, 5 ultraviolet lamps, 6 flame nozzles, 7 fan covers, 8 disinfection cups, 9 exhaust fans, 10 supporting legs, 11 carrying discs, 12 gas tanks, 13 foot-operated control switches and 14 folding soft curtains.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-3, the present invention provides a technical solution: the utility model provides an aseptic inoculation device of tissue culture seedling that dendrobium officinale was used, includes aseptic inoculation hood 1, and aseptic lower fixed surface who connects inoculation hood 1 is connected with supporting leg 10, and the quantity of supporting leg 10 is four, and four supporting legs 10 are the lower surface that the rectangle array set up at aseptic inoculation hood 1.
The sliding tray has been seted up in aseptic inoculation hood 1's front, the inside sliding connection of sliding tray has folding soft curtain 14, the handle hole has been seted up on folding soft curtain 14's surface, the sliding tray has been seted up for four aseptic inoculation hoods 1's front to the quantity of handle hole, the inside sliding connection of sliding tray has folding soft curtain 14, folding soft curtain 14's surface is provided with the handle hole, the quantity of handle hole is four, through setting up four handle holes, can two people operate simultaneously.
The bearing is embedded to the interior diapire of aseptic inoculation box 1, the interior anchor ring fixedly connected with dwang 2 of bearing, the surperficial fixedly connected with bearing block and the branch septum cap 3 respectively of dwang 2, the lateral wall fixedly connected with bearing bar of bearing block, the quantity of bearing bar is two, the last fixed surface of two bearing bars is connected with has thing dish 11, the quantity of branch septum cap 3 is two, two branch septum caps 3 and bearing bar are the cross setting in the inside of aseptic inoculation box 1, divide septum cap 3 through setting up, can be with the internal partitioning of aseptic inoculation box 1 for placing district and inoculation district.
The upper surface of the sterile inoculation box 1 is fixedly connected with a servo motor 4, and the output end of the servo motor 4 is fixedly connected with the upper end of the rotating rod 2.
The fresh air inlet has been seted up to the right side wall of aseptic seed receiving box 1, the surface cover of fresh air inlet is equipped with the filter screen, aseptic inside wall fixedly connected with fan housing 7 that connects seed receiving box 1, aseptic inner diapire fixedly connected with that connects seed receiving box 1 is equipped with the disinfection cup 8 of antiseptic solution, the inside that the air-out end of fan housing 7 extends to the antiseptic solution through the pipeline, the exhaust vent has been seted up to the left wall of aseptic seed receiving box 1, the inner wall fixedly connected with air exhauster 9 of exhaust vent.
The side wall of the supporting leg 10 is fixedly connected with a foot control switch 13, and the exhaust fan 9, the servo motor 4 and the ultraviolet lamp 5 are all coupled with the foot control switch 13.
Through setting up air exhauster 9, disinfection cup 8 and antiseptic solution, can disinfect and filter the air that outside air got into in the box, reached the purpose that prevents that outside bacterium from getting into the inside sterile environment of box.
The working principle is as follows: when the tissue culture seedling sterile inoculation device for the dendrobium officinale is used, firstly, the folding soft curtain 14 on the front side of the sterile inoculation box 1 is opened, a seedling culture test tube and nutrient solution required by inoculation operation are placed in the placement area on the right side of the sterile inoculation box 1, the folding soft curtain 14 is closed, the ultraviolet lamp 5 is opened, the seedling culture test tube and the nutrient solution are subjected to light irradiation disinfection, meanwhile, the exhaust fan 9 is opened, the air inside the box body is kept to circulate, the air entering the box body can be filtered by disinfectant in the disinfection cup 8, one hand passes through the operation hole to place the test tube and the nutrient solution on the carrying disc 11, the other hand controls the servo motor 4 to rotate 180 degrees through the foot control switch 13, the test tube and the nutrient solution on the carrying disc 11 are taken down, the sterile inoculation operation is carried out, and the situation that the placement area on the right side of the sterile inoculation box 1 is polluted due to various, in the in-process of inoculating, keep the aseptic district that test tube mouth, bottleneck or culture dish seam department do not leave flame, prevent to cause the pollution of test tube joint seedling at the in-process of inoculating, then place and pass back through servo motor 4 rotation on carrying thing dish 11 and place the district, reached and prevented that outside bacterium from getting into the inside aseptic environment of box, cause the purpose that test tube inoculation seedling pollutes at the in-process of inoculating.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The utility model provides an aseptic inoculation device of tissue culture seedling that dendrobium officinale used, includes aseptic inoculation case (1), its characterized in that: the inner bottom wall of the sterile inoculation box (1) is embedded with a bearing, the inner annular surface of the bearing is fixedly connected with a rotating rod (2), the surface of the rotating rod (2) is respectively fixedly connected with a bearing block and a separation curtain (3), the lateral wall of the bearing block is fixedly connected with a bearing rod, the upper surface of the sterile inoculation box (1) is fixedly connected with a servo motor (4), the output end of the servo motor (4) is fixedly connected with the upper end of the rotating rod (2), the inner rear wall of the sterile inoculation box (1) is fixedly connected with an ultraviolet lamp (5) and a flame nozzle (6), the right side wall of the sterile inoculation box (1) is provided with an air inlet hole, the outer surface of the air inlet hole is covered with a filter screen, the right side wall of the sterile inoculation box (1) is fixedly connected with an air cover (7), the inner bottom wall of the sterile inoculation box (1) is fixedly connected with a disinfection cup (8) filled with, the air-out end of fan housing (7) passes through the inside that the pipeline extended to the antiseptic solution, the exhaust vent has been seted up to the left wall of aseptic seed receiving box (1), the inner wall fixedly connected with air exhauster (9) of exhaust vent.
2. The aseptic inoculation device of tissue culture seedling for dendrobium officinale according to claim 1, which is characterized in that: the lower fixed surface of aseptic inoculation hood (1) is connected with supporting leg (10), the quantity of supporting leg (10) is four, four supporting leg (10) are the lower surface that the rectangle array set up at aseptic inoculation hood (1).
3. The aseptic inoculation device of tissue culture seedling for dendrobium officinale according to claim 1, which is characterized in that: the number of the bearing rods is two, and the upper surfaces of the two bearing rods are fixedly connected with object carrying discs (11).
4. The aseptic inoculation device of tissue culture seedling for dendrobium officinale according to claim 1, which is characterized in that: the number of the separation curtains (3) is two, and the two separation curtains (3) and the bearing rod are arranged in the sterile inoculation box (1) in a cross structure.
5. The aseptic inoculation device of tissue culture seedling for dendrobium officinale according to claim 1, which is characterized in that: a gas tank (12) is arranged below the sterile inoculation box (1), and the flame nozzle (6) is communicated with the gas tank (12) through a gas pipe.
6. The aseptic inoculation device of tissue culture seedling for dendrobium officinale according to claim 2, which is characterized in that: the lateral wall fixedly connected with foot control switch (13) of supporting leg (10), air exhauster (9), servo motor (4) and ultraviolet lamp (5) all with foot control switch (13) coupling, foot control switch (13) and external power source electric connection.
7. The aseptic inoculation device of tissue culture seedling for dendrobium officinale according to claim 1, which is characterized in that: the front of aseptic inoculation hood (1) has been seted up the sliding tray, the inside sliding connection of sliding tray has folding soft curtain (14), the lateral wall of folding soft curtain (14) is provided with spacing card, the surface of folding soft curtain (14) is provided with the handle hole, the quantity of handle hole is four.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021691212.6U CN213214724U (en) | 2020-08-14 | 2020-08-14 | Aseptic inoculation device of tissue culture seedling that dendrobium officinale used |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021691212.6U CN213214724U (en) | 2020-08-14 | 2020-08-14 | Aseptic inoculation device of tissue culture seedling that dendrobium officinale used |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213214724U true CN213214724U (en) | 2021-05-18 |
Family
ID=75894865
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021691212.6U Expired - Fee Related CN213214724U (en) | 2020-08-14 | 2020-08-14 | Aseptic inoculation device of tissue culture seedling that dendrobium officinale used |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213214724U (en) |
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2020
- 2020-08-14 CN CN202021691212.6U patent/CN213214724U/en not_active Expired - Fee Related
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210518 |