CN105683369A - 变体酶 - Google Patents

变体酶 Download PDF

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Publication number
CN105683369A
CN105683369A CN201480052987.XA CN201480052987A CN105683369A CN 105683369 A CN105683369 A CN 105683369A CN 201480052987 A CN201480052987 A CN 201480052987A CN 105683369 A CN105683369 A CN 105683369A
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CN
China
Prior art keywords
variant
amino acid
substitution
place
acid replacement
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201480052987.XA
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English (en)
Chinese (zh)
Inventor
M·福卡拉基
R·W·霍姆斯
T·卡佩尔
B·R·凯莱门
S·克拉利
S·E·兰茨
I·尼古拉耶夫
德尔 克利 W·范
W·范德尔克利
利斯豪特 J·F·T·范
J·F·T·范利斯豪特
斯提格 桑斯 S·范
S·范斯提格桑斯
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Danisco USA Inc
Original Assignee
Danisco USA Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco USA Inc filed Critical Danisco USA Inc
Publication of CN105683369A publication Critical patent/CN105683369A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Fodder In General (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201480052987.XA 2013-07-29 2014-07-24 变体酶 Pending CN105683369A (zh)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US201361859666P 2013-07-29 2013-07-29
US201361859680P 2013-07-29 2013-07-29
US201361859630P 2013-07-29 2013-07-29
US201361859712P 2013-07-29 2013-07-29
US201361859704P 2013-07-29 2013-07-29
US201361859721P 2013-07-29 2013-07-29
US201361859735P 2013-07-29 2013-07-29
US61/859,735 2013-07-29
US61/859,680 2013-07-29
US61/859,712 2013-07-29
US61/859,666 2013-07-29
US61/859,630 2013-07-29
US61/859,704 2013-07-29
US61/859,721 2013-07-29
PCT/US2014/048075 WO2015017255A1 (en) 2013-07-29 2014-07-24 Variant enzymes

Publications (1)

Publication Number Publication Date
CN105683369A true CN105683369A (zh) 2016-06-15

Family

ID=51299060

Family Applications (3)

Application Number Title Priority Date Filing Date
CN201480052987.XA Pending CN105683369A (zh) 2013-07-29 2014-07-24 变体酶
CN201480052997.3A Pending CN105793417A (zh) 2013-07-29 2014-07-24 变体酶
CN201480053364.4A Pending CN105916981A (zh) 2013-07-29 2014-07-24 变体酶

Family Applications After (2)

Application Number Title Priority Date Filing Date
CN201480052997.3A Pending CN105793417A (zh) 2013-07-29 2014-07-24 变体酶
CN201480053364.4A Pending CN105916981A (zh) 2013-07-29 2014-07-24 变体酶

Country Status (10)

Country Link
US (4) US10479983B2 (https=)
EP (3) EP3027742B1 (https=)
JP (3) JP2016533740A (https=)
CN (3) CN105683369A (https=)
AU (3) AU2014296574A1 (https=)
BR (3) BR112016002038A2 (https=)
DK (2) DK3027742T3 (https=)
MX (3) MX2016001317A (https=)
WO (3) WO2015017254A1 (https=)
ZA (3) ZA201600631B (https=)

Cited By (7)

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CN113913412A (zh) * 2021-10-13 2022-01-11 湖北大学 蛋白酶k突变体及其制备方法
CN116121434A (zh) * 2022-09-26 2023-05-16 四川农业大学 一种控制小麦zip3a锌转运功能的氨基酸位点及分子标记
CN117925577A (zh) * 2024-03-19 2024-04-26 金堂佰信维科技有限公司 提高木聚糖酶活性的方法和木聚糖酶及其应用
CN118910014A (zh) * 2024-09-03 2024-11-08 广州青囊生物科技有限公司 一种生产稀有人参皂苷CK的β-葡萄糖苷酶突变体及其应用
WO2025061210A3 (zh) * 2023-09-19 2025-05-08 中国科学院天津工业生物技术研究所 一种嗜热内切纤维素酶突变体及制备方法
CN120758467A (zh) * 2025-09-11 2025-10-10 中国科学院天津工业生物技术研究所 裂解性多糖单加氧酶突变体及纤维素协同水解方法
CN121204003A (zh) * 2025-10-17 2025-12-26 珠海宝锐生物科技有限公司 一种mmlv酶突变体、制备方法及其应用

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CN110846332B (zh) * 2019-11-29 2022-05-17 怀化学院 一种果胶酶人工序列及其表达方法和应用
CN110846294B (zh) * 2019-11-29 2022-05-17 怀化学院 重组果胶酶及其基因、重组载体、制备方法和应用
CN110747212B (zh) * 2019-11-29 2022-05-17 怀化学院 一种新果胶酶的基因及其蛋白表达、载体和应用
CN111154743B (zh) * 2020-04-01 2021-01-05 黑龙江省农业科学院畜牧兽医分院 突变型纤维素酶及其在玉米青贮中的应用
CN118043454A (zh) 2021-06-18 2024-05-14 国际N&H丹麦有限公司 用于啤酒浑浊减少的蛋白酶
CN114480146B (zh) * 2022-04-15 2022-06-24 中国科学院天津工业生物技术研究所 一种米曲霉菌株在富集铜铁锰锌中的应用
AU2023306398A1 (en) 2022-07-15 2025-01-23 International N&H Denmark Aps Improved enzymatic modification of phospholipids in food
CN119997966A (zh) 2022-09-20 2025-05-13 国际N&H丹麦有限公司 变体抗病毒多肽
AU2024331645A1 (en) 2023-09-01 2026-03-12 International N&H Denmark Aps Proteases and aldc enzymes for beer haze and diacetyl reduction
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CN120464599B (zh) * 2025-07-11 2025-09-12 南京工业大学 Pet水解酶突变体及其应用

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