CN105683369A - 变体酶 - Google Patents
变体酶 Download PDFInfo
- Publication number
- CN105683369A CN105683369A CN201480052987.XA CN201480052987A CN105683369A CN 105683369 A CN105683369 A CN 105683369A CN 201480052987 A CN201480052987 A CN 201480052987A CN 105683369 A CN105683369 A CN 105683369A
- Authority
- CN
- China
- Prior art keywords
- amino acid
- variant
- substitution
- acid substitution
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108090000790 Enzymes Proteins 0.000 title claims description 175
- 102000004190 Enzymes Human genes 0.000 title claims description 174
- 238000000034 method Methods 0.000 claims abstract description 83
- 239000000203 mixture Substances 0.000 claims abstract description 82
- 125000003147 glycosyl group Chemical group 0.000 claims abstract description 47
- 238000006467 substitution reaction Methods 0.000 claims description 1192
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 408
- 229940088598 enzyme Drugs 0.000 claims description 173
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 113
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 110
- 229920001184 polypeptide Polymers 0.000 claims description 109
- 238000003556 assay Methods 0.000 claims description 85
- 241000499912 Trichoderma reesei Species 0.000 claims description 83
- 230000014509 gene expression Effects 0.000 claims description 55
- 229910052700 potassium Inorganic materials 0.000 claims description 52
- 108010059892 Cellulase Proteins 0.000 claims description 49
- 108090000604 Hydrolases Proteins 0.000 claims description 44
- 229910052717 sulfur Inorganic materials 0.000 claims description 44
- 102000004157 Hydrolases Human genes 0.000 claims description 41
- 239000013598 vector Substances 0.000 claims description 41
- 229910052740 iodine Inorganic materials 0.000 claims description 40
- 229910052720 vanadium Inorganic materials 0.000 claims description 38
- 229940106157 cellulase Drugs 0.000 claims description 37
- 229910052731 fluorine Inorganic materials 0.000 claims description 35
- 238000006460 hydrolysis reaction Methods 0.000 claims description 33
- 229910052757 nitrogen Inorganic materials 0.000 claims description 30
- 229910052727 yttrium Inorganic materials 0.000 claims description 30
- 229910052698 phosphorus Inorganic materials 0.000 claims description 28
- 102000040430 polynucleotide Human genes 0.000 claims description 28
- 108091033319 polynucleotide Proteins 0.000 claims description 28
- 239000002157 polynucleotide Substances 0.000 claims description 28
- 150000001413 amino acids Chemical class 0.000 claims description 27
- 230000002538 fungal effect Effects 0.000 claims description 27
- 230000007062 hydrolysis Effects 0.000 claims description 27
- 241000233866 Fungi Species 0.000 claims description 24
- 239000003599 detergent Substances 0.000 claims description 24
- 241000209149 Zea Species 0.000 claims description 23
- 239000000758 substrate Substances 0.000 claims description 23
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 22
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 22
- 235000005822 corn Nutrition 0.000 claims description 22
- 229910052739 hydrogen Inorganic materials 0.000 claims description 22
- 239000013604 expression vector Substances 0.000 claims description 20
- 239000010907 stover Substances 0.000 claims description 15
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 14
- 241000351920 Aspergillus nidulans Species 0.000 claims description 14
- 229910052721 tungsten Inorganic materials 0.000 claims description 14
- 241000228245 Aspergillus niger Species 0.000 claims description 13
- 230000002708 enhancing effect Effects 0.000 claims description 13
- 239000012228 culture supernatant Substances 0.000 claims description 12
- 230000003301 hydrolyzing effect Effects 0.000 claims description 12
- 241000223259 Trichoderma Species 0.000 claims description 10
- 239000002253 acid Substances 0.000 claims description 10
- 230000001580 bacterial effect Effects 0.000 claims description 8
- 241000223261 Trichoderma viride Species 0.000 claims description 7
- 229910021529 ammonia Inorganic materials 0.000 claims description 7
- 241000228212 Aspergillus Species 0.000 claims description 6
- 241000221961 Neurospora crassa Species 0.000 claims description 6
- 241000223260 Trichoderma harzianum Species 0.000 claims description 6
- 238000004113 cell culture Methods 0.000 claims description 6
- 241001513093 Aspergillus awamori Species 0.000 claims description 5
- 241000894120 Trichoderma atroviride Species 0.000 claims description 5
- 241000378866 Trichoderma koningii Species 0.000 claims description 5
- 241000306527 Trichoderma orientale Species 0.000 claims description 5
- 241001149558 Trichoderma virens Species 0.000 claims description 5
- 241001507865 Aspergillus fischeri Species 0.000 claims description 4
- 241001465318 Aspergillus terreus Species 0.000 claims description 4
- 241000123346 Chrysosporium Species 0.000 claims description 4
- 241000223218 Fusarium Species 0.000 claims description 4
- 102220604087 Homeobox protein SIX3_Y49W_mutation Human genes 0.000 claims description 4
- 241001480714 Humicola insolens Species 0.000 claims description 4
- 241000221960 Neurospora Species 0.000 claims description 4
- 241001495429 Thielavia terrestris Species 0.000 claims description 4
- 229910052799 carbon Inorganic materials 0.000 claims description 4
- 102220223293 rs1060502955 Human genes 0.000 claims description 4
- 102220245301 rs1555596673 Human genes 0.000 claims description 4
- 102220005535 rs41322954 Human genes 0.000 claims description 4
- 102220063402 rs786204868 Human genes 0.000 claims description 4
- 102220105537 rs879254604 Human genes 0.000 claims description 4
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims description 3
- 241000228193 Aspergillus clavatus Species 0.000 claims description 3
- 241000228197 Aspergillus flavus Species 0.000 claims description 3
- 241000122821 Aspergillus kawachii Species 0.000 claims description 3
- 240000006439 Aspergillus oryzae Species 0.000 claims description 3
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims description 3
- 241001674013 Chrysosporium lucknowense Species 0.000 claims description 3
- 241001149475 Gaeumannomyces graminis Species 0.000 claims description 3
- 241000223199 Humicola grisea Species 0.000 claims description 3
- 102220577295 Stromal cell-derived factor 1_P23G_mutation Human genes 0.000 claims description 3
- 241001100181 Thermothelomyces heterothallica Species 0.000 claims description 3
- 241000223262 Trichoderma longibrachiatum Species 0.000 claims description 3
- 241000223263 Trichoderma saturnisporum Species 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 239000004173 sunset yellow FCF Substances 0.000 claims description 3
- 241000228215 Aspergillus aculeatus Species 0.000 claims description 2
- 241000228138 Emericella Species 0.000 claims description 2
- 241000896533 Gliocladium Species 0.000 claims description 2
- 241000223198 Humicola Species 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 claims description 2
- 241000228143 Penicillium Species 0.000 claims description 2
- 239000003674 animal food additive Substances 0.000 claims description 2
- 102220492441 Selenoprotein V_F43Y_mutation Human genes 0.000 claims 1
- 102220515765 cAMP-dependent protein kinase inhibitor alpha_N10D_mutation Human genes 0.000 claims 1
- 102200069353 rs8103142 Human genes 0.000 claims 1
- 102220106669 rs879255364 Human genes 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 abstract description 104
- 102000039446 nucleic acids Human genes 0.000 abstract description 72
- 108020004707 nucleic acids Proteins 0.000 abstract description 72
- 102000004316 Oxidoreductases Human genes 0.000 abstract description 2
- 108090000854 Oxidoreductases Proteins 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 120
- 108090000623 proteins and genes Proteins 0.000 description 76
- 230000000694 effects Effects 0.000 description 53
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 45
- 102000004169 proteins and genes Human genes 0.000 description 42
- 229920002678 cellulose Polymers 0.000 description 41
- 239000001913 cellulose Substances 0.000 description 41
- 235000010980 cellulose Nutrition 0.000 description 41
- 102000053602 DNA Human genes 0.000 description 40
- 108020004414 DNA Proteins 0.000 description 40
- 108091028043 Nucleic acid sequence Proteins 0.000 description 34
- 230000035772 mutation Effects 0.000 description 31
- 239000013612 plasmid Substances 0.000 description 28
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 27
- 239000008103 glucose Substances 0.000 description 27
- 108091026890 Coding region Proteins 0.000 description 26
- 238000004519 manufacturing process Methods 0.000 description 23
- 239000010902 straw Substances 0.000 description 20
- 102220577237 Density-regulated protein_G88Y_mutation Human genes 0.000 description 19
- 102220481647 Methylmalonyl-CoA epimerase, mitochondrial_K70T_mutation Human genes 0.000 description 15
- 108010076504 Protein Sorting Signals Proteins 0.000 description 15
- 239000000047 product Substances 0.000 description 15
- 239000000523 sample Substances 0.000 description 15
- 102220005196 rs33954595 Human genes 0.000 description 14
- 108010084185 Cellulases Proteins 0.000 description 13
- 102000005575 Cellulases Human genes 0.000 description 13
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 13
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 13
- 239000003550 marker Substances 0.000 description 13
- 230000008569 process Effects 0.000 description 13
- 235000000346 sugar Nutrition 0.000 description 13
- 239000006228 supernatant Substances 0.000 description 13
- 229920000742 Cotton Polymers 0.000 description 12
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 12
- 238000004128 high performance liquid chromatography Methods 0.000 description 12
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 12
- 238000001542 size-exclusion chromatography Methods 0.000 description 12
- 239000012634 fragment Substances 0.000 description 11
- 239000000463 material Substances 0.000 description 11
- 102220087827 rs28935489 Human genes 0.000 description 11
- 108010038196 saccharide-binding proteins Proteins 0.000 description 11
- -1 sawdust Substances 0.000 description 11
- DLFVBJFMPXGRIB-UHFFFAOYSA-N Acetamide Chemical compound CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 description 10
- 239000002028 Biomass Substances 0.000 description 10
- 125000000539 amino acid group Chemical group 0.000 description 10
- 239000004744 fabric Substances 0.000 description 10
- 102220276607 rs750954949 Human genes 0.000 description 10
- 230000003248 secreting effect Effects 0.000 description 10
- 238000013518 transcription Methods 0.000 description 10
- 230000035897 transcription Effects 0.000 description 10
- 102220621367 Cytochrome P450 2B6_G99E_mutation Human genes 0.000 description 9
- 108091034117 Oligonucleotide Proteins 0.000 description 9
- 238000012986 modification Methods 0.000 description 9
- 230000004048 modification Effects 0.000 description 9
- 230000009466 transformation Effects 0.000 description 9
- 102220535136 4-aminobutyrate aminotransferase, mitochondrial_C321K_mutation Human genes 0.000 description 8
- 102220589098 Caskin-2_L290A_mutation Human genes 0.000 description 8
- 108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 description 8
- 102220545583 Ileal sodium/bile acid cotransporter_N10D_mutation Human genes 0.000 description 8
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 8
- 108010047754 beta-Glucosidase Proteins 0.000 description 8
- 230000003197 catalytic effect Effects 0.000 description 8
- 238000012217 deletion Methods 0.000 description 8
- 230000037430 deletion Effects 0.000 description 8
- 238000011534 incubation Methods 0.000 description 8
- 238000002864 sequence alignment Methods 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 230000014616 translation Effects 0.000 description 8
- 101001035456 Hypocrea jecorina Endoglucanase-4 Proteins 0.000 description 7
- 101100382629 Schizosaccharomyces pombe (strain 972 / ATCC 24843) cbh1 gene Proteins 0.000 description 7
- 102000006995 beta-Glucosidase Human genes 0.000 description 7
- 101150048033 cbh gene Proteins 0.000 description 7
- 230000000295 complement effect Effects 0.000 description 7
- 239000000835 fiber Substances 0.000 description 7
- 108010002430 hemicellulase Proteins 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 238000003752 polymerase chain reaction Methods 0.000 description 7
- 102220228198 rs1064793030 Human genes 0.000 description 7
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 6
- 108020004705 Codon Proteins 0.000 description 6
- 239000004471 Glycine Substances 0.000 description 6
- 238000010240 RT-PCR analysis Methods 0.000 description 6
- 239000008272 agar Substances 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 230000002255 enzymatic effect Effects 0.000 description 6
- 239000000446 fuel Substances 0.000 description 6
- 238000009396 hybridization Methods 0.000 description 6
- 239000002029 lignocellulosic biomass Substances 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 239000007974 sodium acetate buffer Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 239000004094 surface-active agent Substances 0.000 description 6
- 239000004753 textile Substances 0.000 description 6
- 239000002023 wood Substances 0.000 description 6
- 102220575973 Cellular tumor antigen p53_Q167K_mutation Human genes 0.000 description 5
- 102220568823 Dual specificity mitogen-activated protein kinase kinase 1_G65V_mutation Human genes 0.000 description 5
- 102000005548 Hexokinase Human genes 0.000 description 5
- 108700040460 Hexokinases Proteins 0.000 description 5
- 240000007594 Oryza sativa Species 0.000 description 5
- 235000007164 Oryza sativa Nutrition 0.000 description 5
- 229920001131 Pulp (paper) Polymers 0.000 description 5
- 241000209140 Triticum Species 0.000 description 5
- 235000021307 Triticum Nutrition 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 102220353533 c.295G>T Human genes 0.000 description 5
- 108020001778 catalytic domains Proteins 0.000 description 5
- 239000003480 eluent Substances 0.000 description 5
- 239000003623 enhancer Substances 0.000 description 5
- 230000004927 fusion Effects 0.000 description 5
- 230000002068 genetic effect Effects 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 239000002773 nucleotide Substances 0.000 description 5
- 125000003729 nucleotide group Chemical group 0.000 description 5
- 210000001938 protoplast Anatomy 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 235000009566 rice Nutrition 0.000 description 5
- 229920002477 rna polymer Polymers 0.000 description 5
- 102220328515 rs1555583537 Human genes 0.000 description 5
- 102220234869 rs376280361 Human genes 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 238000013519 translation Methods 0.000 description 5
- 210000005253 yeast cell Anatomy 0.000 description 5
- 238000010269 ABTS assay Methods 0.000 description 4
- 102220584076 Atypical kinase COQ8A, mitochondrial_G227I_mutation Human genes 0.000 description 4
- 102220584097 Atypical kinase COQ8A, mitochondrial_N216M_mutation Human genes 0.000 description 4
- 102220574829 Cellular tumor antigen p53_S121F_mutation Human genes 0.000 description 4
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 101710098247 Exoglucanase 1 Proteins 0.000 description 4
- 102220542106 Feline leukemia virus subgroup C receptor-related protein 2_F43Y_mutation Human genes 0.000 description 4
- 102220644920 Manganese-dependent ADP-ribose/CDP-alcohol diphosphatase_Y153Q_mutation Human genes 0.000 description 4
- 108091005804 Peptidases Proteins 0.000 description 4
- 102220537308 Protein NDRG2_P28K_mutation Human genes 0.000 description 4
- 241000235403 Rhizomucor miehei Species 0.000 description 4
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 4
- 238000007792 addition Methods 0.000 description 4
- 239000002154 agricultural waste Substances 0.000 description 4
- 239000002280 amphoteric surfactant Substances 0.000 description 4
- 229960000723 ampicillin Drugs 0.000 description 4
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 239000007795 chemical reaction product Substances 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 239000002299 complementary DNA Substances 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 230000001404 mediated effect Effects 0.000 description 4
- 238000002703 mutagenesis Methods 0.000 description 4
- 231100000350 mutagenesis Toxicity 0.000 description 4
- 238000005457 optimization Methods 0.000 description 4
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 230000010076 replication Effects 0.000 description 4
- 102220024774 rs199473393 Human genes 0.000 description 4
- 102220011686 rs202004587 Human genes 0.000 description 4
- 102220005160 rs33954595 Human genes 0.000 description 4
- 102200089582 rs5030804 Human genes 0.000 description 4
- 102220033266 rs61751410 Human genes 0.000 description 4
- 102220026200 rs63750213 Human genes 0.000 description 4
- 102220101425 rs878853501 Human genes 0.000 description 4
- 238000002741 site-directed mutagenesis Methods 0.000 description 4
- 239000007858 starting material Substances 0.000 description 4
- 150000008163 sugars Chemical class 0.000 description 4
- 230000002103 transcriptional effect Effects 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 3
- 102220489582 Acetyl-CoA acetyltransferase, cytosolic_H66G_mutation Human genes 0.000 description 3
- 241000609240 Ambelania acida Species 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 102220582742 Biotinidase_L76D_mutation Human genes 0.000 description 3
- 102220552800 Cellular tumor antigen p53_T230S_mutation Human genes 0.000 description 3
- 102220501444 Cytosolic iron-sulfur assembly component 3_D73A_mutation Human genes 0.000 description 3
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 3
- 101710112457 Exoglucanase Proteins 0.000 description 3
- 102100027285 Fanconi anemia group B protein Human genes 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 238000010268 HPLC based assay Methods 0.000 description 3
- 229920002488 Hemicellulose Polymers 0.000 description 3
- 102220588900 Histone H3-like centromeric protein A_S68Q_mutation Human genes 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
- 241000228168 Penicillium sp. Species 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 3
- 241000209504 Poaceae Species 0.000 description 3
- 239000004793 Polystyrene Substances 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 102220538948 SUMO-conjugating enzyme UBC9_F22A_mutation Human genes 0.000 description 3
- 241001494489 Thielavia Species 0.000 description 3
- 102220482238 Tripartite motif-containing protein 74_W13R_mutation Human genes 0.000 description 3
- 239000012190 activator Substances 0.000 description 3
- 125000000217 alkyl group Chemical group 0.000 description 3
- 101150069003 amdS gene Proteins 0.000 description 3
- 239000003945 anionic surfactant Substances 0.000 description 3
- 239000010905 bagasse Substances 0.000 description 3
- 102220348392 c.107T>C Human genes 0.000 description 3
- 102220350650 c.295G>C Human genes 0.000 description 3
- 238000011088 calibration curve Methods 0.000 description 3
- 101150052795 cbh-1 gene Proteins 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000001186 cumulative effect Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 150000002016 disaccharides Chemical class 0.000 description 3
- 231100000673 dose–response relationship Toxicity 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 230000001747 exhibiting effect Effects 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 125000000524 functional group Chemical group 0.000 description 3
- 229930182478 glucoside Natural products 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 238000002844 melting Methods 0.000 description 3
- 230000008018 melting Effects 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 239000002736 nonionic surfactant Substances 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 229940096055 prax Drugs 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 101150054232 pyrG gene Proteins 0.000 description 3
- 102220083492 rs104894094 Human genes 0.000 description 3
- 102220223286 rs1060501211 Human genes 0.000 description 3
- 102200162479 rs139548132 Human genes 0.000 description 3
- 102220278884 rs1554569658 Human genes 0.000 description 3
- 102220285619 rs1555059081 Human genes 0.000 description 3
- 102220283196 rs1555594803 Human genes 0.000 description 3
- 102200082903 rs35140348 Human genes 0.000 description 3
- 102220049431 rs587784257 Human genes 0.000 description 3
- 102220058925 rs750058876 Human genes 0.000 description 3
- 102220097478 rs751124745 Human genes 0.000 description 3
- 102220103395 rs765325777 Human genes 0.000 description 3
- 102220218709 rs765437517 Human genes 0.000 description 3
- 102220071198 rs79632685 Human genes 0.000 description 3
- 102220083086 rs863224621 Human genes 0.000 description 3
- 102220202090 rs864622596 Human genes 0.000 description 3
- 102220090145 rs875989858 Human genes 0.000 description 3
- 102220097933 rs876659871 Human genes 0.000 description 3
- 102220098934 rs878854576 Human genes 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000010561 standard procedure Methods 0.000 description 3
- 238000011426 transformation method Methods 0.000 description 3
- 229910021642 ultra pure water Inorganic materials 0.000 description 3
- 239000012498 ultrapure water Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- FYGDTMLNYKFZSV-WFYNLLPOSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,3s,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-WFYNLLPOSA-N 0.000 description 2
- 102220472850 ADP-ribosylation factor-like protein 13B_T35N_mutation Human genes 0.000 description 2
- 102220604157 Adenosine deaminase_K64A_mutation Human genes 0.000 description 2
- 241000931365 Ampelodesmos mauritanicus Species 0.000 description 2
- 102220465452 Angiogenin_K64Q_mutation Human genes 0.000 description 2
- 102220629004 Anterior gradient protein 2 homolog_D47N_mutation Human genes 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000228257 Aspergillus sp. Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 102220618447 Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe_P89A_mutation Human genes 0.000 description 2
- 229920002498 Beta-glucan Polymers 0.000 description 2
- 102220582679 Biotinidase_L76K_mutation Human genes 0.000 description 2
- 102220504735 Borealin_T230K_mutation Human genes 0.000 description 2
- 238000009010 Bradford assay Methods 0.000 description 2
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 2
- 235000006008 Brassica napus var napus Nutrition 0.000 description 2
- 240000000385 Brassica napus var. napus Species 0.000 description 2
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
- 102220485758 Carbohydrate sulfotransferase 12_P52H_mutation Human genes 0.000 description 2
- 102220499807 Carbonic anhydrase 2_N62H_mutation Human genes 0.000 description 2
- 101710095524 Cellodextrinase Proteins 0.000 description 2
- 102220575971 Cellular tumor antigen p53_Q167L_mutation Human genes 0.000 description 2
- 102220591062 Cellular tumor antigen p53_S183E_mutation Human genes 0.000 description 2
- 102220574054 Cellular tumor antigen p53_S185C_mutation Human genes 0.000 description 2
- 102220574052 Cellular tumor antigen p53_S185G_mutation Human genes 0.000 description 2
- 102220574053 Cellular tumor antigen p53_S185I_mutation Human genes 0.000 description 2
- 102220522551 Cellular tumor antigen p53_S185T_mutation Human genes 0.000 description 2
- 102220552822 Cellular tumor antigen p53_V225F_mutation Human genes 0.000 description 2
- 102220617576 Cerebellin-3_A48N_mutation Human genes 0.000 description 2
- 241000123350 Chrysosporium sp. Species 0.000 description 2
- 241001429695 Colletotrichum graminicola Species 0.000 description 2
- 102220553754 Cyclic GMP-AMP synthase_T35E_mutation Human genes 0.000 description 2
- 239000001692 EU approved anti-caking agent Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 102220542220 Eukaryotic peptide chain release factor GTP-binding subunit ERF3B_P23T_mutation Human genes 0.000 description 2
- 101710098246 Exoglucanase 2 Proteins 0.000 description 2
- 102220548187 F-box-like/WD repeat-containing protein TBL1X_L94E_mutation Human genes 0.000 description 2
- 102220565429 Fidgetin-like protein 1_T61D_mutation Human genes 0.000 description 2
- 102220627377 Forkhead box protein P3_L76A_mutation Human genes 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 241001149959 Fusarium sp. Species 0.000 description 2
- 102220642725 Glutathione peroxidase 2_A37L_mutation Human genes 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 102000005744 Glycoside Hydrolases Human genes 0.000 description 2
- 108010031186 Glycoside Hydrolases Proteins 0.000 description 2
- 102220498335 HLA class II histocompatibility antigen, DR beta 3 chain_L39Y_mutation Human genes 0.000 description 2
- 102220577829 Hepatocyte nuclear factor 3-alpha_N96A_mutation Human genes 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 102220487046 Histone H4 transcription factor_E25Q_mutation Human genes 0.000 description 2
- 102220526817 Homeobox protein MSX-1_W13A_mutation Human genes 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 101001067705 Hypocrea jecorina (strain QM6a) Endoglucanase-7 Proteins 0.000 description 2
- 102220475716 Immunoglobulin kappa variable 2-24_T21K_mutation Human genes 0.000 description 2
- 108091092195 Intron Proteins 0.000 description 2
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 2
- 241000209082 Lolium Species 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 102220506708 Meckel syndrome type 1 protein_L39F_mutation Human genes 0.000 description 2
- 240000003433 Miscanthus floridulus Species 0.000 description 2
- 108020005196 Mitochondrial DNA Proteins 0.000 description 2
- 102220538112 Modulator of macroautophagy TMEM150B_A63H_mutation Human genes 0.000 description 2
- 102220556763 Myosin light chain kinase, smooth muscle_S68A_mutation Human genes 0.000 description 2
- 102220599464 Neuropeptides B/W receptor type 1_P19Q_mutation Human genes 0.000 description 2
- 238000000636 Northern blotting Methods 0.000 description 2
- 102220504520 Organic solute transporter subunit alpha_T20K_mutation Human genes 0.000 description 2
- 102220581198 Oxidized purine nucleoside triphosphate hydrolase_D47P_mutation Human genes 0.000 description 2
- 102220585282 PH domain leucine-rich repeat-containing protein phosphatase 1_A60L_mutation Human genes 0.000 description 2
- 241001520808 Panicum virgatum Species 0.000 description 2
- 102220528963 Pentraxin-related protein PTX3_A48D_mutation Human genes 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 235000014676 Phragmites communis Nutrition 0.000 description 2
- 102220637623 Phytanoyl-CoA hydroxylase-interacting protein-like_L76I_mutation Human genes 0.000 description 2
- 102220468523 Podocin_P89T_mutation Human genes 0.000 description 2
- 102220613852 Presenilin-1_V82E_mutation Human genes 0.000 description 2
- 102220467909 Protein Dok-7_V32A_mutation Human genes 0.000 description 2
- 102220472196 Protein Wnt-2_L322A_mutation Human genes 0.000 description 2
- 102220474362 Protein ZNRD2_T21M_mutation Human genes 0.000 description 2
- 102220586238 Protein downstream neighbor of Son_N51L_mutation Human genes 0.000 description 2
- 102220540010 Protein phosphatase 1 regulatory subunit 1A_T35D_mutation Human genes 0.000 description 2
- 102220524118 Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1_D47R_mutation Human genes 0.000 description 2
- 102220544141 Pyrin_T20A_mutation Human genes 0.000 description 2
- 102220506924 Ras-related C3 botulinum toxin substrate 1_T35S_mutation Human genes 0.000 description 2
- 102220573234 Ras-related protein Ral-A_A48W_mutation Human genes 0.000 description 2
- 102220631896 Regulator of G-protein signaling 2_Q78H_mutation Human genes 0.000 description 2
- 102220487583 Retinitis pigmentosa 1-like 1 protein_P19H_mutation Human genes 0.000 description 2
- 102220514466 SERTA domain-containing protein 1_N98Q_mutation Human genes 0.000 description 2
- 102220482487 Serine/threonine-protein kinase Nek8_K64M_mutation Human genes 0.000 description 2
- 102220504613 Serine/threonine-protein kinase receptor R3_N96S_mutation Human genes 0.000 description 2
- 108020004682 Single-Stranded DNA Proteins 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- HIWPGCMGAMJNRG-ACCAVRKYSA-N Sophorose Natural products O([C@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HIWPGCMGAMJNRG-ACCAVRKYSA-N 0.000 description 2
- 102220590630 Spindlin-1_T20N_mutation Human genes 0.000 description 2
- 241000187747 Streptomyces Species 0.000 description 2
- 102220517476 Stress-induced-phosphoprotein 1_T230V_mutation Human genes 0.000 description 2
- 102220565024 Survival motor neuron protein_L39A_mutation Human genes 0.000 description 2
- 102220563641 T-box brain protein 1_Q50A_mutation Human genes 0.000 description 2
- 108091036066 Three prime untranslated region Proteins 0.000 description 2
- 102220509962 Thromboxane A2 receptor_V80E_mutation Human genes 0.000 description 2
- 102220598990 Transcriptional coactivator YAP1_V80A_mutation Human genes 0.000 description 2
- 102220522584 Ubiquitin-fold modifier 1_A48Q_mutation Human genes 0.000 description 2
- 102220473189 Zinc finger protein 658_S68Y_mutation Human genes 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 102000004139 alpha-Amylases Human genes 0.000 description 2
- 108090000637 alpha-Amylases Proteins 0.000 description 2
- 229940024171 alpha-amylase Drugs 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 235000020054 awamori Nutrition 0.000 description 2
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- HIWPGCMGAMJNRG-UHFFFAOYSA-N beta-sophorose Natural products OC1C(O)C(CO)OC(O)C1OC1C(O)C(O)C(O)C(CO)O1 HIWPGCMGAMJNRG-UHFFFAOYSA-N 0.000 description 2
- 239000007844 bleaching agent Substances 0.000 description 2
- 210000004899 c-terminal region Anatomy 0.000 description 2
- 102220363170 c.112G>A Human genes 0.000 description 2
- 102220364119 c.148C>A Human genes 0.000 description 2
- 102220395971 c.158A>T Human genes 0.000 description 2
- 102220411694 c.203C>T Human genes 0.000 description 2
- 102220355684 c.203G>A Human genes 0.000 description 2
- 102220361662 c.208A>C Human genes 0.000 description 2
- 102200005749 c.305C>A Human genes 0.000 description 2
- 102220362050 c.35T>C Human genes 0.000 description 2
- 102220346324 c.47C>A Human genes 0.000 description 2
- 102220364294 c.481G>T Human genes 0.000 description 2
- 102220344907 c.491G>A Human genes 0.000 description 2
- 102220359961 c.583G>A Human genes 0.000 description 2
- 102220364012 c.64T>A Human genes 0.000 description 2
- 102220419249 c.652A>G Human genes 0.000 description 2
- 102220419571 c.82C>T Human genes 0.000 description 2
- 102220362929 c.869T>C Human genes 0.000 description 2
- 102220406055 c.889A>G Human genes 0.000 description 2
- 102220350935 c.896A>G Human genes 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 238000012219 cassette mutagenesis Methods 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 239000003093 cationic surfactant Substances 0.000 description 2
- 101150114858 cbh2 gene Proteins 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 239000003502 gasoline Substances 0.000 description 2
- 150000008131 glucosides Chemical class 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- PSFDQSOCUJVVGF-UHFFFAOYSA-N harman Chemical compound C12=CC=CC=C2NC2=C1C=CN=C2C PSFDQSOCUJVVGF-UHFFFAOYSA-N 0.000 description 2
- 229940059442 hemicellulase Drugs 0.000 description 2
- 239000010903 husk Substances 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 150000002772 monosaccharides Chemical class 0.000 description 2
- 238000010606 normalization Methods 0.000 description 2
- 125000001209 o-nitrophenyl group Chemical group [H]C1=C([H])C(*)=C(C([H])=C1[H])[N+]([O-])=O 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 239000013600 plasmid vector Substances 0.000 description 2
- 210000002706 plastid Anatomy 0.000 description 2
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 239000013587 production medium Substances 0.000 description 2
- 210000001236 prokaryotic cell Anatomy 0.000 description 2
- 239000012521 purified sample Substances 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 238000003259 recombinant expression Methods 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 102220261077 rs1012113887 Human genes 0.000 description 2
- 102200069896 rs104893961 Human genes 0.000 description 2
- 102200078032 rs104895296 Human genes 0.000 description 2
- 102220195409 rs1057518507 Human genes 0.000 description 2
- 102220218869 rs1060501626 Human genes 0.000 description 2
- 102220224251 rs1060502313 Human genes 0.000 description 2
- 102220221760 rs1060503638 Human genes 0.000 description 2
- 102220231555 rs1064797160 Human genes 0.000 description 2
- 102220232795 rs1085307566 Human genes 0.000 description 2
- 102220008213 rs116840785 Human genes 0.000 description 2
- 102220317282 rs116840785 Human genes 0.000 description 2
- 102220316328 rs1315405264 Human genes 0.000 description 2
- 102200109065 rs137852792 Human genes 0.000 description 2
- 102220294141 rs138270964 Human genes 0.000 description 2
- 102220141773 rs138846036 Human genes 0.000 description 2
- 102220283741 rs1413319082 Human genes 0.000 description 2
- 102220162299 rs143723093 Human genes 0.000 description 2
- 102220115142 rs145133167 Human genes 0.000 description 2
- 102220123641 rs147067921 Human genes 0.000 description 2
- 102220175214 rs148982578 Human genes 0.000 description 2
- 102220323254 rs150140303 Human genes 0.000 description 2
- 102200106059 rs150607408 Human genes 0.000 description 2
- 102220313227 rs1553179312 Human genes 0.000 description 2
- 102220277090 rs1553411488 Human genes 0.000 description 2
- 102220285404 rs1553644231 Human genes 0.000 description 2
- 102220258544 rs1553644251 Human genes 0.000 description 2
- 102220261398 rs1554200995 Human genes 0.000 description 2
- 102220283437 rs1555738391 Human genes 0.000 description 2
- 102220298424 rs1555921926 Human genes 0.000 description 2
- 102220290299 rs1555972495 Human genes 0.000 description 2
- 102200108151 rs17683011 Human genes 0.000 description 2
- 102200025796 rs179363881 Human genes 0.000 description 2
- 102200025794 rs179363887 Human genes 0.000 description 2
- 102220208907 rs179468 Human genes 0.000 description 2
- 102220027111 rs1799977 Human genes 0.000 description 2
- 102220216923 rs199645805 Human genes 0.000 description 2
- 102220112467 rs199804735 Human genes 0.000 description 2
- 102220329805 rs199816697 Human genes 0.000 description 2
- 102200049218 rs201865375 Human genes 0.000 description 2
- 102220005136 rs33918778 Human genes 0.000 description 2
- 102220005153 rs33922018 Human genes 0.000 description 2
- 102200082929 rs33924146 Human genes 0.000 description 2
- 102220005185 rs33924146 Human genes 0.000 description 2
- 102220005200 rs33954595 Human genes 0.000 description 2
- 102200082904 rs33974228 Human genes 0.000 description 2
- 102200118253 rs34151786 Human genes 0.000 description 2
- 102220032873 rs367543128 Human genes 0.000 description 2
- 102220082159 rs369666769 Human genes 0.000 description 2
- 102220281242 rs370230541 Human genes 0.000 description 2
- 102220090146 rs370608001 Human genes 0.000 description 2
- 102200108629 rs371524413 Human genes 0.000 description 2
- 102220097787 rs372201428 Human genes 0.000 description 2
- 102220057687 rs373291490 Human genes 0.000 description 2
- 102220261561 rs374050750 Human genes 0.000 description 2
- 102220297938 rs375722926 Human genes 0.000 description 2
- 102220323583 rs375754042 Human genes 0.000 description 2
- 102220230417 rs376280361 Human genes 0.000 description 2
- 102220028381 rs386352349 Human genes 0.000 description 2
- 102220009473 rs397507189 Human genes 0.000 description 2
- 102220021058 rs397508898 Human genes 0.000 description 2
- 102220114368 rs397517248 Human genes 0.000 description 2
- 102220001970 rs4684677 Human genes 0.000 description 2
- 102220266953 rs539994406 Human genes 0.000 description 2
- 102220323552 rs541477171 Human genes 0.000 description 2
- 102220104434 rs551236465 Human genes 0.000 description 2
- 102200108627 rs563378859 Human genes 0.000 description 2
- 102220037047 rs575910805 Human genes 0.000 description 2
- 102200131343 rs57966821 Human genes 0.000 description 2
- 102200006402 rs587778189 Human genes 0.000 description 2
- 102220040118 rs587778189 Human genes 0.000 description 2
- 102220059910 rs587778189 Human genes 0.000 description 2
- 102220027326 rs587779074 Human genes 0.000 description 2
- 102220041676 rs587779102 Human genes 0.000 description 2
- 102220276576 rs587780746 Human genes 0.000 description 2
- 102220044405 rs587781277 Human genes 0.000 description 2
- 102220044832 rs587781608 Human genes 0.000 description 2
- 102220044994 rs587781741 Human genes 0.000 description 2
- 102220047561 rs587783059 Human genes 0.000 description 2
- 102220248786 rs59191035 Human genes 0.000 description 2
- 102200124675 rs606231410 Human genes 0.000 description 2
- 102200110495 rs61748529 Human genes 0.000 description 2
- 102200108303 rs730881994 Human genes 0.000 description 2
- 102220025838 rs74953290 Human genes 0.000 description 2
- 102220105380 rs750126678 Human genes 0.000 description 2
- 102200005763 rs754806883 Human genes 0.000 description 2
- 102220248953 rs756928158 Human genes 0.000 description 2
- 102220062000 rs759149207 Human genes 0.000 description 2
- 102220094198 rs759589301 Human genes 0.000 description 2
- 102220323551 rs771587118 Human genes 0.000 description 2
- 102220058779 rs771641237 Human genes 0.000 description 2
- 102220298053 rs772216708 Human genes 0.000 description 2
- 102200104506 rs772773208 Human genes 0.000 description 2
- 102220244682 rs778284147 Human genes 0.000 description 2
- 102220218086 rs781721739 Human genes 0.000 description 2
- 102220062246 rs786201754 Human genes 0.000 description 2
- 102200107874 rs786202717 Human genes 0.000 description 2
- 102220059033 rs786203178 Human genes 0.000 description 2
- 102220063395 rs786204944 Human genes 0.000 description 2
- 102220067329 rs794727244 Human genes 0.000 description 2
- 102220021465 rs80356936 Human genes 0.000 description 2
- 102220009549 rs80357190 Human genes 0.000 description 2
- 102220082859 rs863224240 Human genes 0.000 description 2
- 102220083825 rs863224593 Human genes 0.000 description 2
- 102220094201 rs876658278 Human genes 0.000 description 2
- 102220096755 rs876660744 Human genes 0.000 description 2
- 102220093865 rs876660804 Human genes 0.000 description 2
- 102220093587 rs876661106 Human genes 0.000 description 2
- 102220103817 rs878853955 Human genes 0.000 description 2
- 102220100736 rs878854061 Human genes 0.000 description 2
- 102220102670 rs878854090 Human genes 0.000 description 2
- 102200037679 rs879254635 Human genes 0.000 description 2
- 102220106374 rs879255290 Human genes 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- HIWPGCMGAMJNRG-RTPHMHGBSA-N sophorose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)OC(O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HIWPGCMGAMJNRG-RTPHMHGBSA-N 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 101150016309 trpC gene Proteins 0.000 description 2
- 238000000108 ultra-filtration Methods 0.000 description 2
- 239000013603 viral vector Substances 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- 238000002424 x-ray crystallography Methods 0.000 description 2
- DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 1
- IAYJZWFYUSNIPN-KFRZSCGFSA-N (2s,3r,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](OC=2C=CC(=CC=2)[N+]([O-])=O)[C@H](O)[C@H]1O IAYJZWFYUSNIPN-KFRZSCGFSA-N 0.000 description 1
- 102220492454 2'-5'-oligoadenylate synthase 3_T35L_mutation Human genes 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- 102220583922 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase_I75A_mutation Human genes 0.000 description 1
- 102220543795 39S ribosomal protein L15, mitochondrial_P29L_mutation Human genes 0.000 description 1
- 102220575769 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase 9_N62Q_mutation Human genes 0.000 description 1
- 102220598603 5-hydroxytryptamine receptor 1E_I54A_mutation Human genes 0.000 description 1
- 102220496116 5-hydroxytryptamine receptor 3B_V80R_mutation Human genes 0.000 description 1
- 102220592281 7SK snRNA methylphosphate capping enzyme_K64S_mutation Human genes 0.000 description 1
- 102220554198 APC membrane recruitment protein 1_E25A_mutation Human genes 0.000 description 1
- 102220553837 APC membrane recruitment protein 1_K58A_mutation Human genes 0.000 description 1
- 102220510685 APC membrane recruitment protein 1_T21H_mutation Human genes 0.000 description 1
- 102220510686 APC membrane recruitment protein 1_T21Y_mutation Human genes 0.000 description 1
- 102220547941 ASNSD1 upstream open reading frame protein_F43A_mutation Human genes 0.000 description 1
- 102220547927 ASNSD1 upstream open reading frame protein_F43W_mutation Human genes 0.000 description 1
- 102220549849 ATP-binding cassette sub-family D member 1_A95D_mutation Human genes 0.000 description 1
- 102220489594 Acetyl-CoA acetyltransferase, cytosolic_H66A_mutation Human genes 0.000 description 1
- 108010013043 Acetylesterase Proteins 0.000 description 1
- 102100022900 Actin, cytoplasmic 1 Human genes 0.000 description 1
- 102220582488 Actin, cytoplasmic 1_W79E_mutation Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 102220511400 Adenine DNA glycosylase_G186E_mutation Human genes 0.000 description 1
- 102220511442 Adenine DNA glycosylase_W128R_mutation Human genes 0.000 description 1
- 102220573882 Adenosine receptor A2a_T21L_mutation Human genes 0.000 description 1
- 102220490385 Adenosylhomocysteinase 3_D73R_mutation Human genes 0.000 description 1
- 102220506381 Adenylyl-sulfate kinase_L76Q_mutation Human genes 0.000 description 1
- 101100433464 African swine fever virus (strain Badajoz 1971 Vero-adapted) BA71V-013 gene Proteins 0.000 description 1
- 102220486135 Alpha-galactosidase A_C56F_mutation Human genes 0.000 description 1
- 102220486134 Alpha-galactosidase A_C56Y_mutation Human genes 0.000 description 1
- 102220485993 Alpha-galactosidase A_D92H_mutation Human genes 0.000 description 1
- 102220557157 Alstrom syndrome protein 1_I75Y_mutation Human genes 0.000 description 1
- 102220484545 Amiloride-sensitive sodium channel subunit delta_P28N_mutation Human genes 0.000 description 1
- 102220470955 Amiloride-sensitive sodium channel subunit delta_Y24G_mutation Human genes 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 102220621805 Apolipoprotein C-III_P28Q_mutation Human genes 0.000 description 1
- 101710152845 Arabinogalactan endo-beta-1,4-galactanase Proteins 0.000 description 1
- 102220547456 Arginine and glutamate-rich protein 1_S68R_mutation Human genes 0.000 description 1
- 102220602660 Argininosuccinate synthase_V69A_mutation Human genes 0.000 description 1
- 241000722807 Arthrobotrys oligospora Species 0.000 description 1
- 102000035101 Aspartic proteases Human genes 0.000 description 1
- 108091005502 Aspartic proteases Proteins 0.000 description 1
- 101000961203 Aspergillus awamori Glucoamylase Proteins 0.000 description 1
- 241001225321 Aspergillus fumigatus Species 0.000 description 1
- 101000757144 Aspergillus niger Glucoamylase Proteins 0.000 description 1
- 101900127796 Aspergillus oryzae Glucoamylase Proteins 0.000 description 1
- 102220561506 Autophagy protein 5_K58M_mutation Human genes 0.000 description 1
- 102220561183 Autophagy-related protein 2 homolog A_V82R_mutation Human genes 0.000 description 1
- 102220618215 Autophagy-related protein 9A_F77A_mutation Human genes 0.000 description 1
- 102220567514 BICD family-like cargo adapter 2_D38A_mutation Human genes 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 102220590217 Barttin_C56S_mutation Human genes 0.000 description 1
- 102220590222 Barttin_V32G_mutation Human genes 0.000 description 1
- 102100032487 Beta-mannosidase Human genes 0.000 description 1
- 102220504782 Beta-ureidopropionase_N51A_mutation Human genes 0.000 description 1
- 241000680806 Blastobotrys adeninivorans Species 0.000 description 1
- 240000008564 Boehmeria nivea Species 0.000 description 1
- 101001047514 Bos taurus Lethal(2) giant larvae protein homolog 1 Proteins 0.000 description 1
- 241000605900 Butyrivibrio fibrisolvens Species 0.000 description 1
- 102220615367 C-C chemokine receptor type 5_Y14F_mutation Human genes 0.000 description 1
- 102220523643 C-C motif chemokine 2_V32E_mutation Human genes 0.000 description 1
- 102220616652 CCAAT/enhancer-binding protein alpha_I55A_mutation Human genes 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102220499814 Carbonic anhydrase 2_N62A_mutation Human genes 0.000 description 1
- 102220499813 Carbonic anhydrase 2_N62D_mutation Human genes 0.000 description 1
- 102220499804 Carbonic anhydrase 2_N62L_mutation Human genes 0.000 description 1
- 102220499800 Carbonic anhydrase 2_N62V_mutation Human genes 0.000 description 1
- 102220548517 Carbonic anhydrase 3_K64H_mutation Human genes 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 102220571117 Carboxypeptidase M_V32K_mutation Human genes 0.000 description 1
- 102220534612 Caspase recruitment domain-containing protein 9_K58D_mutation Human genes 0.000 description 1
- 102220571876 Cellular tumor antigen p53_A161P_mutation Human genes 0.000 description 1
- 102220571874 Cellular tumor antigen p53_A161T_mutation Human genes 0.000 description 1
- 102220571883 Cellular tumor antigen p53_A161V_mutation Human genes 0.000 description 1
- 102220523387 Cellular tumor antigen p53_L194I_mutation Human genes 0.000 description 1
- 102220523368 Cellular tumor antigen p53_L194V_mutation Human genes 0.000 description 1
- 102220522416 Cellular tumor antigen p53_P190H_mutation Human genes 0.000 description 1
- 102220551923 Cellular tumor antigen p53_P222A_mutation Human genes 0.000 description 1
- 102220551874 Cellular tumor antigen p53_P222Q_mutation Human genes 0.000 description 1
- 102220551865 Cellular tumor antigen p53_P222R_mutation Human genes 0.000 description 1
- 102220551875 Cellular tumor antigen p53_P222T_mutation Human genes 0.000 description 1
- 102220584205 Cellular tumor antigen p53_P87Q_mutation Human genes 0.000 description 1
- 102220571911 Cellular tumor antigen p53_Q167H_mutation Human genes 0.000 description 1
- 102220575981 Cellular tumor antigen p53_Q167R_mutation Human genes 0.000 description 1
- 102220568566 Cellular tumor antigen p53_R156G_mutation Human genes 0.000 description 1
- 102220572058 Cellular tumor antigen p53_R156L_mutation Human genes 0.000 description 1
- 102220572054 Cellular tumor antigen p53_R156S_mutation Human genes 0.000 description 1
- 102220591061 Cellular tumor antigen p53_S183A_mutation Human genes 0.000 description 1
- 102220573732 Cellular tumor antigen p53_S183P_mutation Human genes 0.000 description 1
- 102220574051 Cellular tumor antigen p53_S185R_mutation Human genes 0.000 description 1
- 102220552789 Cellular tumor antigen p53_T230N_mutation Human genes 0.000 description 1
- 102220552811 Cellular tumor antigen p53_V225A_mutation Human genes 0.000 description 1
- 102220552821 Cellular tumor antigen p53_V225D_mutation Human genes 0.000 description 1
- 102220552823 Cellular tumor antigen p53_V225G_mutation Human genes 0.000 description 1
- 102220550486 Cellular tumor antigen p53_V225L_mutation Human genes 0.000 description 1
- 229920003043 Cellulose fiber Polymers 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 102220591969 Chitinase domain-containing protein 1_G99W_mutation Human genes 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 102220591878 Cholinephosphotransferase 1_C56V_mutation Human genes 0.000 description 1
- 241001517488 Clavus Species 0.000 description 1
- 101800004419 Cleaved form Proteins 0.000 description 1
- 102220627890 Coagulation factor IX_C97S_mutation Human genes 0.000 description 1
- 108700010070 Codon Usage Proteins 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 102220519282 Conserved oligomeric Golgi complex subunit 3_P89D_mutation Human genes 0.000 description 1
- 229910021591 Copper(I) chloride Inorganic materials 0.000 description 1
- 240000000491 Corchorus aestuans Species 0.000 description 1
- 235000011777 Corchorus aestuans Nutrition 0.000 description 1
- 235000010862 Corchorus capsularis Nutrition 0.000 description 1
- 102220516971 Core-binding factor subunit beta_I54F_mutation Human genes 0.000 description 1
- 241000235646 Cyberlindnera jadinii Species 0.000 description 1
- 102100033270 Cyclin-dependent kinase inhibitor 1 Human genes 0.000 description 1
- 102220504544 Cyclin-dependent kinase inhibitor 2A_H66Y_mutation Human genes 0.000 description 1
- 102220592997 Cyclin-dependent kinase inhibitor 2A_L94Q_mutation Human genes 0.000 description 1
- 102100034770 Cyclin-dependent kinase inhibitor 3 Human genes 0.000 description 1
- 102220541230 Cystatin-D_H86R_mutation Human genes 0.000 description 1
- 102220621822 Cytochrome P450 7A1_H86N_mutation Human genes 0.000 description 1
- 102220557952 Cytosolic carboxypeptidase 2_I90R_mutation Human genes 0.000 description 1
- 102220501462 Cytosolic iron-sulfur assembly component 3_S26H_mutation Human genes 0.000 description 1
- 125000002353 D-glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 102220602356 DAZ-associated protein 1_F43D_mutation Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 102220597350 DNA-directed RNA polymerase III subunit RPC1_I55S_mutation Human genes 0.000 description 1
- 241000235035 Debaryomyces Species 0.000 description 1
- 102220577241 Density-regulated protein_A36L_mutation Human genes 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 102220559691 Differentially expressed in FDCP 8 homolog_A71R_mutation Human genes 0.000 description 1
- 102220560628 Differentially expressed in FDCP 8 homolog_I55R_mutation Human genes 0.000 description 1
- 102220559697 Differentially expressed in FDCP 8 homolog_I75R_mutation Human genes 0.000 description 1
- 241000199914 Dinophyceae Species 0.000 description 1
- 102220499922 DnaJ homolog subfamily C member 2_P83A_mutation Human genes 0.000 description 1
- 102220489019 Dual specificity protein phosphatase 13 isoform A_N51I_mutation Human genes 0.000 description 1
- 102220537009 Dynamin-1-like protein_Y49A_mutation Human genes 0.000 description 1
- 102220529221 E3 ubiquitin-protein ligase RBX1_N59C_mutation Human genes 0.000 description 1
- 102220501058 E3 ubiquitin-protein ligase TRAIP_Y17F_mutation Human genes 0.000 description 1
- 102220508359 E3 ubiquitin-protein ligase XIAP_V80D_mutation Human genes 0.000 description 1
- 102220466999 EH domain-binding protein 1-like protein 1_G33K_mutation Human genes 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102220468422 Elongin-A_S26M_mutation Human genes 0.000 description 1
- 241000088541 Emericella sp. Species 0.000 description 1
- 101710147028 Endo-beta-1,4-galactanase Proteins 0.000 description 1
- 101710126559 Endoglucanase EG-II Proteins 0.000 description 1
- 241000178951 Endomyces Species 0.000 description 1
- 102220557059 Epiplakin_P81D_mutation Human genes 0.000 description 1
- 108090000371 Esterases Proteins 0.000 description 1
- 102220516902 Estrogen-related receptor gamma_S45D_mutation Human genes 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 102220471819 Eukaryotic translation initiation factor 4E_S68K_mutation Human genes 0.000 description 1
- 102220548188 F-box-like/WD repeat-containing protein TBL1X_I90A_mutation Human genes 0.000 description 1
- 102220625728 FAD-linked sulfhydryl oxidase ALR_T74D_mutation Human genes 0.000 description 1
- 102220497518 Fatty acid-binding protein, intestinal_L39G_mutation Human genes 0.000 description 1
- 102220543204 Fibronectin_Q15L_mutation Human genes 0.000 description 1
- 102220552923 Fidgetin-like protein 1_V44P_mutation Human genes 0.000 description 1
- 241000223195 Fusarium graminearum Species 0.000 description 1
- 102220480248 G-protein coupled receptor family C group 6 member A_Q78A_mutation Human genes 0.000 description 1
- 102220642466 Gap junction alpha-8 protein_K64D_mutation Human genes 0.000 description 1
- 102220590588 Gap junction beta-1 protein_P87L_mutation Human genes 0.000 description 1
- 102220590587 Gap junction beta-1 protein_P87S_mutation Human genes 0.000 description 1
- 102220508004 Gasdermin-E_D18V_mutation Human genes 0.000 description 1
- 102220613915 Germ cell-less protein-like 1_S45T_mutation Human genes 0.000 description 1
- 241000768015 Gliocladium sp. Species 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 102000053187 Glucuronidase Human genes 0.000 description 1
- 108010060309 Glucuronidase Proteins 0.000 description 1
- 102220619581 Glycine receptor subunit alpha-1_T35I_mutation Human genes 0.000 description 1
- 102220485773 Glycophorin-A_L94I_mutation Human genes 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 102220576054 HLA class I histocompatibility antigen, B alpha chain_I90K_mutation Human genes 0.000 description 1
- 102220576061 HLA class I histocompatibility antigen, B alpha chain_I90N_mutation Human genes 0.000 description 1
- 102220541599 HLA class II histocompatibility antigen, DQ beta 1 chain_P87R_mutation Human genes 0.000 description 1
- 101100295959 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) arcB gene Proteins 0.000 description 1
- 241001154911 Harpullia aeruginosa Species 0.000 description 1
- 102220628249 Hemoglobin subunit alpha_N10T_mutation Human genes 0.000 description 1
- 102220605091 Hemoglobin subunit beta_Y199E_mutation Human genes 0.000 description 1
- 102220622874 Hemoglobin subunit delta_S45N_mutation Human genes 0.000 description 1
- 102220622835 Hemoglobin subunit delta_V12G_mutation Human genes 0.000 description 1
- 102220636373 Hemojuvelin_C321W_mutation Human genes 0.000 description 1
- 108091027305 Heteroduplex Proteins 0.000 description 1
- 102220634001 Heterogeneous nuclear ribonucleoprotein K_A60Y_mutation Human genes 0.000 description 1
- 241000375795 Heterospora Species 0.000 description 1
- 229920000209 Hexadimethrine bromide Polymers 0.000 description 1
- 108010093488 His-His-His-His-His-His Proteins 0.000 description 1
- 102220605276 Histone deacetylase 3_G11W_mutation Human genes 0.000 description 1
- 101100439050 Homo sapiens CDKN3 gene Proteins 0.000 description 1
- 101001050472 Homo sapiens Integral membrane protein 2A Proteins 0.000 description 1
- 102220621658 Homogentisate 1,2-dioxygenase_D18N_mutation Human genes 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 108010020056 Hydrogenase Proteins 0.000 description 1
- 239000004354 Hydroxyethyl cellulose Substances 0.000 description 1
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 description 1
- 102220507144 Inactive N-acetylated-alpha-linked acidic dipeptidase-like protein 2_S45H_mutation Human genes 0.000 description 1
- 102220635681 Inner centromere protein_F22R_mutation Human genes 0.000 description 1
- 102220492673 Integrin alpha-3_H86S_mutation Human genes 0.000 description 1
- 102220630803 Interferon alpha-8_A67E_mutation Human genes 0.000 description 1
- 102220627710 Interferon alpha-8_D73V_mutation Human genes 0.000 description 1
- 102220478978 Interleukin-4 receptor subunit alpha_N98A_mutation Human genes 0.000 description 1
- 102220633778 Kelch-like protein 12_A60K_mutation Human genes 0.000 description 1
- 102220525658 Keratin-associated protein 26-1_S26Y_mutation Human genes 0.000 description 1
- 241000235058 Komagataella pastoris Species 0.000 description 1
- 102220500176 Leucine rich adaptor protein 1-like_K70G_mutation Human genes 0.000 description 1
- 102220498484 Leucine-rich repeat flightless-interacting protein 1_S68C_mutation Human genes 0.000 description 1
- 102220617354 Leucine-rich repeat-containing protein 34_V91W_mutation Human genes 0.000 description 1
- 229920002097 Lichenin Polymers 0.000 description 1
- 101100504421 Limnoria quadripunctata GH7B gene Proteins 0.000 description 1
- 241000208202 Linaceae Species 0.000 description 1
- 235000004431 Linum usitatissimum Nutrition 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 102220522517 Low-density lipoprotein receptor-related protein 5_W79R_mutation Human genes 0.000 description 1
- 229920000433 Lyocell Polymers 0.000 description 1
- 102220558197 Lysophospholipase D GDPD1_Y17C_mutation Human genes 0.000 description 1
- 102220536073 Lysophospholipase_K58L_mutation Human genes 0.000 description 1
- 102100033448 Lysosomal alpha-glucosidase Human genes 0.000 description 1
- 101710154526 Lytic chitin monooxygenase Proteins 0.000 description 1
- 102220507166 MICOS complex subunit MIC25_A95S_mutation Human genes 0.000 description 1
- 241001667714 Macrosporium Species 0.000 description 1
- 108010059896 Manganese peroxidase Proteins 0.000 description 1
- 229920000057 Mannan Polymers 0.000 description 1
- 102220514146 Mediator of DNA damage checkpoint protein 1_G11Q_mutation Human genes 0.000 description 1
- 102220514139 Mediator of DNA damage checkpoint protein 1_G72N_mutation Human genes 0.000 description 1
- 102220476997 Membrane-spanning 4-domains subfamily A member 13_I7V_mutation Human genes 0.000 description 1
- 102220507624 Menin_L39W_mutation Human genes 0.000 description 1
- 102000003792 Metallothionein Human genes 0.000 description 1
- 108090000157 Metallothionein Proteins 0.000 description 1
- 102220573092 Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial_W79A_mutation Human genes 0.000 description 1
- 102220573083 Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial_W79F_mutation Human genes 0.000 description 1
- 102220578111 Microtubule-associated proteins 1A/1B light chain 3B_H57D_mutation Human genes 0.000 description 1
- 102220624190 Minor histocompatibility antigen H13_N10Q_mutation Human genes 0.000 description 1
- 102220548791 Mitochondrial import inner membrane translocase subunit TIM16_Y93G_mutation Human genes 0.000 description 1
- 241001203365 Myceliophthora sp. Species 0.000 description 1
- 102220528496 Myelin protein P0_V32F_mutation Human genes 0.000 description 1
- 102220523847 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13_A16P_mutation Human genes 0.000 description 1
- 102220628415 NPC intracellular cholesterol transporter 1_N41A_mutation Human genes 0.000 description 1
- 241001507755 Neosartorya Species 0.000 description 1
- 102220592394 Neurogenin-1_N41P_mutation Human genes 0.000 description 1
- 241000088436 Neurospora sp. Species 0.000 description 1
- 102220602031 Neutrophil elastase_V80G_mutation Human genes 0.000 description 1
- 102220592907 Nitric oxide synthase, endothelial_N27Q_mutation Human genes 0.000 description 1
- 102220592493 Non-homologous end-joining factor 1_D40P_mutation Human genes 0.000 description 1
- 241000320412 Ogataea angusta Species 0.000 description 1
- 102220482961 Ornithine decarboxylase antizyme 1_C97V_mutation Human genes 0.000 description 1
- 102220575633 Outer dense fiber protein 4_W34R_mutation Human genes 0.000 description 1
- 102220581210 Oxidized purine nucleoside triphosphate hydrolase_G42I_mutation Human genes 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 102220475056 POTE ankyrin domain family member C_H86D_mutation Human genes 0.000 description 1
- 102220642303 PTB domain-containing engulfment adapter protein 1_K58E_mutation Human genes 0.000 description 1
- 102220642348 PTB domain-containing engulfment adapter protein 1_Q78P_mutation Human genes 0.000 description 1
- 239000002033 PVDF binder Substances 0.000 description 1
- 102220553233 Pancreatic prohormone_D40K_mutation Human genes 0.000 description 1
- 102220543410 Parathyroid hormone_A16R_mutation Human genes 0.000 description 1
- 102000010292 Peptide Elongation Factor 1 Human genes 0.000 description 1
- 108010077524 Peptide Elongation Factor 1 Proteins 0.000 description 1
- 102220519486 Peptidyl-prolyl cis-trans isomerase FKBP2_C97Y_mutation Human genes 0.000 description 1
- 241001542817 Phaffia Species 0.000 description 1
- 241000081271 Phaffia rhodozyma Species 0.000 description 1
- 244000081757 Phalaris arundinacea Species 0.000 description 1
- 241000222393 Phanerochaete chrysosporium Species 0.000 description 1
- 102220637661 Phenylalanine-4-hydroxylase_A309D_mutation Human genes 0.000 description 1
- 102220503245 Phosphoribosylformylglycinamidine synthase_D92L_mutation Human genes 0.000 description 1
- 244000273256 Phragmites communis Species 0.000 description 1
- 102220620245 Pituitary-specific positive transcription factor 1_N51D_mutation Human genes 0.000 description 1
- 102220539591 Piwi-like protein 1_G72D_mutation Human genes 0.000 description 1
- 102220539584 Piwi-like protein 1_Y93D_mutation Human genes 0.000 description 1
- 102220577567 Potassium channel subfamily K member 18_N96Q_mutation Human genes 0.000 description 1
- 102220634786 Potassium voltage-gated channel subfamily A member 3_F77W_mutation Human genes 0.000 description 1
- 102220613592 Potassium voltage-gated channel subfamily D member 3_D73M_mutation Human genes 0.000 description 1
- 102220628658 Presenilin-2_D40E_mutation Human genes 0.000 description 1
- 102220635826 Probable C-mannosyltransferase DPY19L1_S26C_mutation Human genes 0.000 description 1
- 102220553089 Proenkephalin-A_F77Y_mutation Human genes 0.000 description 1
- 102220549519 Prokineticin-1_I55L_mutation Human genes 0.000 description 1
- 102220517106 Protease-associated domain-containing protein 1_D40L_mutation Human genes 0.000 description 1
- 102220604415 Proteasomal ubiquitin receptor ADRM1_Y93A_mutation Human genes 0.000 description 1
- 102220604423 Proteasomal ubiquitin receptor ADRM1_Y93K_mutation Human genes 0.000 description 1
- 102220604355 Proteasomal ubiquitin receptor ADRM1_Y93L_mutation Human genes 0.000 description 1
- 102220479474 Proteasome inhibitor PI31 subunit_I90E_mutation Human genes 0.000 description 1
- 102220470514 Proteasome subunit beta type-3_V82A_mutation Human genes 0.000 description 1
- 102220470512 Proteasome subunit beta type-3_V82G_mutation Human genes 0.000 description 1
- 102220556894 Protein ALEX_N10A_mutation Human genes 0.000 description 1
- 102220547354 Protein APCDD1_A63W_mutation Human genes 0.000 description 1
- 102220606029 Protein C19orf12_A48P_mutation Human genes 0.000 description 1
- 102220519982 Protein DEK_A16S_mutation Human genes 0.000 description 1
- 102220580420 Protein FAM74A4/A6_D92I_mutation Human genes 0.000 description 1
- 102220493006 Protein Flattop_F22Y_mutation Human genes 0.000 description 1
- 102220554819 Protein Mpv17_D92K_mutation Human genes 0.000 description 1
- 102220537295 Protein NDRG2_V31P_mutation Human genes 0.000 description 1
- 102220478946 Protein WFDC9_N27T_mutation Human genes 0.000 description 1
- 102220613793 Protein diaphanous homolog 2_D92E_mutation Human genes 0.000 description 1
- 102220466316 Protein jagged-1_G33V_mutation Human genes 0.000 description 1
- 102220619487 Protein mago nashi homolog_D73K_mutation Human genes 0.000 description 1
- 102220582411 Protein max_L94P_mutation Human genes 0.000 description 1
- 102220640206 Proton channel OTOP1_S26P_mutation Human genes 0.000 description 1
- 102220516347 Pterin-4-alpha-carbinolamine dehydratase 2_Y24E_mutation Human genes 0.000 description 1
- 229920001218 Pullulan Polymers 0.000 description 1
- 102220550889 Purkinje cell protein 2 homolog_A37I_mutation Human genes 0.000 description 1
- 102220587526 Putative uncharacterized protein FLJ13197_N62W_mutation Human genes 0.000 description 1
- 102220544143 Pyrin_T20D_mutation Human genes 0.000 description 1
- 102220619404 RNA polymerase I-specific transcription initiation factor RRN3_W84A_mutation Human genes 0.000 description 1
- 102220567299 Rab3 GTPase-activating protein non-catalytic subunit_Y17A_mutation Human genes 0.000 description 1
- 102220542742 Ras and Rab interactor 1_W79Y_mutation Human genes 0.000 description 1
- 101000985291 Rattus norvegicus Neuron-specific calcium-binding protein hippocalcin Proteins 0.000 description 1
- 101000987490 Rattus norvegicus Phosphatidylethanolamine-binding protein 1 Proteins 0.000 description 1
- 229920000297 Rayon Polymers 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102220631894 Regulator of G-protein signaling 2_D40Y_mutation Human genes 0.000 description 1
- 102220518617 Repressor of RNA polymerase III transcription MAF1 homolog_S68D_mutation Human genes 0.000 description 1
- 108091027981 Response element Proteins 0.000 description 1
- 102220494160 Reticulocalbin-1_D73Y_mutation Human genes 0.000 description 1
- 102220555348 Retinal-specific phospholipid-transporting ATPase ABCA4_G72V_mutation Human genes 0.000 description 1
- 102220485931 Rhodopsin_P23L_mutation Human genes 0.000 description 1
- 102220466109 Ribitol-5-phosphate xylosyltransferase 1_Y17L_mutation Human genes 0.000 description 1
- 102220524642 Ribosomal protein S6 kinase beta-2_I30A_mutation Human genes 0.000 description 1
- 101100394022 Robillarda sp. (strain Y-20) eg 1 gene Proteins 0.000 description 1
- 241000192026 Ruminococcus flavefaciens Species 0.000 description 1
- 108091006627 SLC12A9 Proteins 0.000 description 1
- 102220509535 SMAD5 antisense gene protein 1_D47S_mutation Human genes 0.000 description 1
- 102220509530 SMAD5 antisense gene protein 1_S26G_mutation Human genes 0.000 description 1
- 241000235060 Scheffersomyces stipitis Species 0.000 description 1
- 241001123649 Schwanniomyces polymorphus Species 0.000 description 1
- 102220568439 Segment polarity protein dishevelled homolog DVL-2_Y17D_mutation Human genes 0.000 description 1
- 102220613767 Semaphorin-3D_N98G_mutation Human genes 0.000 description 1
- 102220528260 Sialic acid-binding Ig-like lectin 12_P81H_mutation Human genes 0.000 description 1
- 102220496163 Signal peptide, CUB and EGF-like domain-containing protein 3_C97W_mutation Human genes 0.000 description 1
- 102220517225 Signal transducing adapter molecule 1_F77E_mutation Human genes 0.000 description 1
- 102220517259 Signal transducing adapter molecule 1_W34F_mutation Human genes 0.000 description 1
- 102220471968 Single-stranded DNA cytosine deaminase_K10A_mutation Human genes 0.000 description 1
- 102220633723 Sodium/potassium-transporting ATPase subunit alpha-2_P83R_mutation Human genes 0.000 description 1
- 102220609637 Solute carrier family 22 member 12_G65W_mutation Human genes 0.000 description 1
- 102220616771 Son of sevenless homolog 1_H86A_mutation Human genes 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 102220610352 Spectrin alpha chain, erythrocytic 1_V31A_mutation Human genes 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 101100370749 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) trpC1 gene Proteins 0.000 description 1
- 241000187412 Streptomyces plicatus Species 0.000 description 1
- 102220600050 Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial_N152T_mutation Human genes 0.000 description 1
- 102220599995 Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial_S68P_mutation Human genes 0.000 description 1
- 102220607237 Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial_V154T_mutation Human genes 0.000 description 1
- 102220578366 Sulfite oxidase, mitochondrial_G42K_mutation Human genes 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 102220538772 Superoxide dismutase [Cu-Zn]_L39R_mutation Human genes 0.000 description 1
- 102220608724 Suppressor of cytokine signaling 2_D53R_mutation Human genes 0.000 description 1
- 102220578461 Suppressor of fused homolog_V69D_mutation Human genes 0.000 description 1
- 102220565025 Survival motor neuron protein_W34A_mutation Human genes 0.000 description 1
- 102220565188 Synphilin-1_P81N_mutation Human genes 0.000 description 1
- 102220579120 Syntaxin-3_P19A_mutation Human genes 0.000 description 1
- 102220579003 Syntaxin-3_Q15A_mutation Human genes 0.000 description 1
- 102220484150 T cell receptor alpha variable 34_A67R_mutation Human genes 0.000 description 1
- 102220484147 T cell receptor alpha variable 34_A67W_mutation Human genes 0.000 description 1
- 102220533994 T cell receptor beta variable 11-3_P83D_mutation Human genes 0.000 description 1
- 102220563666 T-box brain protein 1_Y24A_mutation Human genes 0.000 description 1
- 102220536493 THAP domain-containing protein 1_H57A_mutation Human genes 0.000 description 1
- 102220521912 THAP domain-containing protein 1_H57N_mutation Human genes 0.000 description 1
- 102220500146 Target of EGR1 protein 1_N27A_mutation Human genes 0.000 description 1
- 102220500145 Target of EGR1 protein 1_N27D_mutation Human genes 0.000 description 1
- 102220537940 Taste receptor type 2 member 40_H66K_mutation Human genes 0.000 description 1
- 241001344092 Tetraspora <Myxozoa> Species 0.000 description 1
- 241001313536 Thermothelomyces thermophila Species 0.000 description 1
- 102220554577 Thrombospondin-type laminin G domain and EAR repeat-containing protein_D18A_mutation Human genes 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102220567526 Transferrin receptor protein 1_T21F_mutation Human genes 0.000 description 1
- 241001557886 Trichoderma sp. Species 0.000 description 1
- 102220586119 Tubulin polymerization-promoting protein_S45E_mutation Human genes 0.000 description 1
- 102220602374 Ubiquitin carboxyl-terminal hydrolase 4_D92R_mutation Human genes 0.000 description 1
- 102220524273 Ubiquitin-related modifier 1_Y14E_mutation Human genes 0.000 description 1
- 102220586580 Uncharacterized protein FAM241A_P46Q_mutation Human genes 0.000 description 1
- 102220635556 Vacuolar protein sorting-associated protein 33A_N62I_mutation Human genes 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 102220559220 Voltage-dependent L-type calcium channel subunit alpha-1C_V31R_mutation Human genes 0.000 description 1
- 102220582435 Voltage-dependent T-type calcium channel subunit alpha-1H_N59A_mutation Human genes 0.000 description 1
- 102220582434 Voltage-dependent T-type calcium channel subunit alpha-1H_N59H_mutation Human genes 0.000 description 1
- 102220582436 Voltage-dependent T-type calcium channel subunit alpha-1H_N59K_mutation Human genes 0.000 description 1
- 102220532186 WW domain-binding protein 11_K58S_mutation Human genes 0.000 description 1
- 241000235015 Yarrowia lipolytica Species 0.000 description 1
- 102220516076 Zinc finger protein 37 homolog_V31E_mutation Human genes 0.000 description 1
- 241000588902 Zymomonas mobilis Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 108010048241 acetamidase Proteins 0.000 description 1
- 108010093941 acetylxylan esterase Proteins 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 101150069317 alcA gene Proteins 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 125000003342 alkenyl group Chemical group 0.000 description 1
- 125000002947 alkylene group Chemical group 0.000 description 1
- 108010028144 alpha-Glucosidases Proteins 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 101150078331 ama-1 gene Proteins 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 101150008194 argB gene Proteins 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- OHDRQQURAXLVGJ-AXMZSLBLSA-N azane;(2z)-3-ethyl-2-[(z)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N\N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-AXMZSLBLSA-N 0.000 description 1
- OHDRQQURAXLVGJ-UHFFFAOYSA-N azane;3-ethyl-2-[(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C1=NN=C1SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 108010055059 beta-Mannosidase Proteins 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000002051 biphasic effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 102220363675 c.109G>C Human genes 0.000 description 1
- 102220396061 c.125G>T Human genes 0.000 description 1
- 102220356195 c.139G>T Human genes 0.000 description 1
- 102220351005 c.143C>T Human genes 0.000 description 1
- 102220360316 c.151G>T Human genes 0.000 description 1
- 102220364631 c.155C>T Human genes 0.000 description 1
- 102220349339 c.160A>G Human genes 0.000 description 1
- 102220351562 c.164T>A Human genes 0.000 description 1
- 102200006386 c.179C>T Human genes 0.000 description 1
- 102220393259 c.184G>T Human genes 0.000 description 1
- 102220350876 c.190A>G Human genes 0.000 description 1
- 102220348732 c.223A>G Human genes 0.000 description 1
- 102220417893 c.226C>G Human genes 0.000 description 1
- 102220414544 c.227G>A Human genes 0.000 description 1
- 102200006094 c.241C>A Human genes 0.000 description 1
- 102220346513 c.241C>G Human genes 0.000 description 1
- 102220389033 c.253C>G Human genes 0.000 description 1
- 102220355440 c.257A>T Human genes 0.000 description 1
- 102220415151 c.265_266delCCinsAA Human genes 0.000 description 1
- 102220425405 c.278A>T Human genes 0.000 description 1
- 102220388270 c.283G>A Human genes 0.000 description 1
- 102220359541 c.284C>T Human genes 0.000 description 1
- 102220368314 c.295G>A Human genes 0.000 description 1
- 102220351621 c.311C>T Human genes 0.000 description 1
- 102220407727 c.314A>G Human genes 0.000 description 1
- 102220403029 c.31G>A Human genes 0.000 description 1
- 102220349575 c.32G>A Human genes 0.000 description 1
- 102220353718 c.32G>A Human genes 0.000 description 1
- 102220408215 c.385G>T Human genes 0.000 description 1
- 102220367028 c.41A>G Human genes 0.000 description 1
- 102220354936 c.436G>A Human genes 0.000 description 1
- 102200108625 c.467G>C Human genes 0.000 description 1
- 102220350736 c.475A>G Human genes 0.000 description 1
- 102220417837 c.533T>A Human genes 0.000 description 1
- 102220411671 c.539T>G Human genes 0.000 description 1
- 102220359955 c.54C>A Human genes 0.000 description 1
- 102220362606 c.557G>T Human genes 0.000 description 1
- 102200091217 c.563T>A Human genes 0.000 description 1
- 102200091221 c.563T>C Human genes 0.000 description 1
- 102200093825 c.593T>C Human genes 0.000 description 1
- 102220359924 c.61T>G Human genes 0.000 description 1
- 102220357245 c.628A>G Human genes 0.000 description 1
- 102220351014 c.62C>A Human genes 0.000 description 1
- 102220359948 c.641C>T Human genes 0.000 description 1
- 102220355653 c.67A>C Human genes 0.000 description 1
- 102220363351 c.68C>A Human genes 0.000 description 1
- 102220414135 c.692G>C Human genes 0.000 description 1
- 102220402990 c.925G>C Human genes 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000022534 cell killing Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 108010080434 cephalosporin-C deacetylase Proteins 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 101150089280 cip2 gene Proteins 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 230000004186 co-expression Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000002967 competitive immunoassay Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- OXBLHERUFWYNTN-UHFFFAOYSA-M copper(I) chloride Chemical compound [Cu]Cl OXBLHERUFWYNTN-UHFFFAOYSA-M 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000002447 crystallographic data Methods 0.000 description 1
- 230000001461 cytolytic effect Effects 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000002298 density-gradient ultracentrifugation Methods 0.000 description 1
- 230000009025 developmental regulation Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000004851 dishwashing Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 102220500056 eIF5-mimic protein 2_Q15E_mutation Human genes 0.000 description 1
- 101150066032 egl-1 gene Proteins 0.000 description 1
- 101150003727 egl2 gene Proteins 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 1
- 150000002170 ethers Chemical class 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 108010041969 feruloyl esterase Proteins 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000012224 gene deletion Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000012268 genome sequencing Methods 0.000 description 1
- 230000009229 glucose formation Effects 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 108091072218 glycosyl hydrolase 61 family Proteins 0.000 description 1
- 101150073906 gpdA gene Proteins 0.000 description 1
- 101150095733 gpsA gene Proteins 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000011121 hardwood Substances 0.000 description 1
- 230000008642 heat stress Effects 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 238000010169 landfilling Methods 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 108010062085 ligninase Proteins 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 125000005647 linker group Chemical group 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 101150039489 lysZ gene Proteins 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000001471 micro-filtration Methods 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 239000006151 minimal media Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 101150095344 niaD gene Proteins 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- TVMXDCGIABBOFY-UHFFFAOYSA-N octane Chemical compound CCCCCCCC TVMXDCGIABBOFY-UHFFFAOYSA-N 0.000 description 1
- JRZJOMJEPLMPRA-UHFFFAOYSA-N olefin Natural products CCCCCCCC=C JRZJOMJEPLMPRA-UHFFFAOYSA-N 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 125000000636 p-nitrophenyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1*)[N+]([O-])=O 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000006072 paste Substances 0.000 description 1
- 108020004410 pectinesterase Proteins 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 210000004896 polypeptide structure Anatomy 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- KCXFHTAICRTXLI-UHFFFAOYSA-N propane-1-sulfonic acid Chemical compound CCCS(O)(=O)=O KCXFHTAICRTXLI-UHFFFAOYSA-N 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 235000019423 pullulan Nutrition 0.000 description 1
- 101150089778 pyr-4 gene Proteins 0.000 description 1
- 101150095482 pyr2 gene Proteins 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 239000002964 rayon Substances 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 102200087972 rs1042821 Human genes 0.000 description 1
- 102220033750 rs104886462 Human genes 0.000 description 1
- 102220033751 rs104886463 Human genes 0.000 description 1
- 102200141512 rs104893768 Human genes 0.000 description 1
- 102220004588 rs104893797 Human genes 0.000 description 1
- 102200089625 rs104893829 Human genes 0.000 description 1
- 102200005748 rs104894094 Human genes 0.000 description 1
- 102200147641 rs104894534 Human genes 0.000 description 1
- 102200014179 rs104894576 Human genes 0.000 description 1
- 102200086203 rs104894623 Human genes 0.000 description 1
- 102200074430 rs104894786 Human genes 0.000 description 1
- 102200153433 rs104894817 Human genes 0.000 description 1
- 102200032988 rs104894959 Human genes 0.000 description 1
- 102200039307 rs1057516099 Human genes 0.000 description 1
- 102220196457 rs1057518971 Human genes 0.000 description 1
- 102220198136 rs1057519882 Human genes 0.000 description 1
- 102200105979 rs1057519998 Human genes 0.000 description 1
- 102200106019 rs1057519998 Human genes 0.000 description 1
- 102200106022 rs1057519998 Human genes 0.000 description 1
- 102220198436 rs1057520005 Human genes 0.000 description 1
- 102220198437 rs1057520006 Human genes 0.000 description 1
- 102220206869 rs1057522105 Human genes 0.000 description 1
- 102220205652 rs1057522121 Human genes 0.000 description 1
- 102220210314 rs1057524439 Human genes 0.000 description 1
- 102220215398 rs1060500701 Human genes 0.000 description 1
- 102220220120 rs1060500786 Human genes 0.000 description 1
- 102220222804 rs1060501218 Human genes 0.000 description 1
- 102220222800 rs1060501247 Human genes 0.000 description 1
- 102220218176 rs1060501269 Human genes 0.000 description 1
- 102220219331 rs1060501710 Human genes 0.000 description 1
- 102220224023 rs1060502096 Human genes 0.000 description 1
- 102220212853 rs1060502671 Human genes 0.000 description 1
- 102220215726 rs1060503344 Human genes 0.000 description 1
- 102220218873 rs1060503403 Human genes 0.000 description 1
- 102220323023 rs1060503403 Human genes 0.000 description 1
- 102220214820 rs1060503554 Human genes 0.000 description 1
- 102220223818 rs1060503785 Human genes 0.000 description 1
- 102220088306 rs1064792858 Human genes 0.000 description 1
- 102220230047 rs1064793318 Human genes 0.000 description 1
- 102220228938 rs1064793498 Human genes 0.000 description 1
- 102220229710 rs1064793867 Human genes 0.000 description 1
- 102220226177 rs1064793870 Human genes 0.000 description 1
- 102220226391 rs1064794271 Human genes 0.000 description 1
- 102220226091 rs1064794928 Human genes 0.000 description 1
- 102220226396 rs1064794951 Human genes 0.000 description 1
- 102200108402 rs1064795691 Human genes 0.000 description 1
- 102220232874 rs1085307493 Human genes 0.000 description 1
- 102220005338 rs111033604 Human genes 0.000 description 1
- 102200012539 rs111033647 Human genes 0.000 description 1
- 102200012541 rs111033647 Human genes 0.000 description 1
- 102200012575 rs111033652 Human genes 0.000 description 1
- 102200062292 rs1114167623 Human genes 0.000 description 1
- 102220040807 rs111826855 Human genes 0.000 description 1
- 102220236150 rs1131691586 Human genes 0.000 description 1
- 102220236572 rs1131692262 Human genes 0.000 description 1
- 102220264795 rs11552823 Human genes 0.000 description 1
- 102220326980 rs1165815510 Human genes 0.000 description 1
- 102200039331 rs121434318 Human genes 0.000 description 1
- 102220324615 rs1216148236 Human genes 0.000 description 1
- 102200001998 rs121908108 Human genes 0.000 description 1
- 102200037435 rs121908539 Human genes 0.000 description 1
- 102200058947 rs121909540 Human genes 0.000 description 1
- 102200131583 rs121912433 Human genes 0.000 description 1
- 102200131581 rs121912434 Human genes 0.000 description 1
- 102220006171 rs121912729 Human genes 0.000 description 1
- 102200044937 rs121913396 Human genes 0.000 description 1
- 102200044897 rs121913407 Human genes 0.000 description 1
- 102220198042 rs121913407 Human genes 0.000 description 1
- 102200044886 rs121913409 Human genes 0.000 description 1
- 102220197786 rs121913409 Human genes 0.000 description 1
- 102200000406 rs121917854 Human genes 0.000 description 1
- 102200115876 rs121918077 Human genes 0.000 description 1
- 102200115315 rs121918084 Human genes 0.000 description 1
- 102200115798 rs121918098 Human genes 0.000 description 1
- 102220003859 rs121918521 Human genes 0.000 description 1
- 102200065638 rs121918610 Human genes 0.000 description 1
- 102200156917 rs121964893 Human genes 0.000 description 1
- 102200043451 rs121965069 Human genes 0.000 description 1
- 102220281532 rs1221633501 Human genes 0.000 description 1
- 102220324075 rs1231596455 Human genes 0.000 description 1
- 102220285854 rs1319163924 Human genes 0.000 description 1
- 102220276885 rs1330621664 Human genes 0.000 description 1
- 102220253692 rs1332910409 Human genes 0.000 description 1
- 102220344095 rs1333025395 Human genes 0.000 description 1
- 102220326429 rs1352220583 Human genes 0.000 description 1
- 102200109061 rs137852792 Human genes 0.000 description 1
- 102200140789 rs137853080 Human genes 0.000 description 1
- 102220313252 rs1386345719 Human genes 0.000 description 1
- 102220059799 rs139233015 Human genes 0.000 description 1
- 102220285599 rs1412024666 Human genes 0.000 description 1
- 102220036887 rs141690729 Human genes 0.000 description 1
- 102220058133 rs141690729 Human genes 0.000 description 1
- 102220041209 rs142135772 Human genes 0.000 description 1
- 102220045982 rs142498771 Human genes 0.000 description 1
- 102220147983 rs143129444 Human genes 0.000 description 1
- 102200109060 rs1438095083 Human genes 0.000 description 1
- 102220276830 rs1444375563 Human genes 0.000 description 1
- 102220258393 rs1445512588 Human genes 0.000 description 1
- 102220067359 rs144739747 Human genes 0.000 description 1
- 102200127347 rs144890720 Human genes 0.000 description 1
- 102200106726 rs146340390 Human genes 0.000 description 1
- 102220270377 rs146735953 Human genes 0.000 description 1
- 102220327423 rs148273392 Human genes 0.000 description 1
- 102220014835 rs148791216 Human genes 0.000 description 1
- 102220046362 rs150478207 Human genes 0.000 description 1
- 102220086250 rs150478207 Human genes 0.000 description 1
- 102220011563 rs150973053 Human genes 0.000 description 1
- 102220276544 rs1553127659 Human genes 0.000 description 1
- 102220276911 rs1553350837 Human genes 0.000 description 1
- 102220289163 rs1553352536 Human genes 0.000 description 1
- 102220277072 rs1553408398 Human genes 0.000 description 1
- 102220316484 rs1553619979 Human genes 0.000 description 1
- 102220277482 rs1553640297 Human genes 0.000 description 1
- 102220277596 rs1553640334 Human genes 0.000 description 1
- 102220277510 rs1553644227 Human genes 0.000 description 1
- 102220317871 rs1554069530 Human genes 0.000 description 1
- 102220309985 rs1554139870 Human genes 0.000 description 1
- 102220250535 rs1554399226 Human genes 0.000 description 1
- 102220278845 rs1554568336 Human genes 0.000 description 1
- 102220251096 rs1554841961 Human genes 0.000 description 1
- 102220285575 rs1554888103 Human genes 0.000 description 1
- 102220266287 rs1554890348 Human genes 0.000 description 1
- 102220267180 rs1555017253 Human genes 0.000 description 1
- 102220265722 rs1555055319 Human genes 0.000 description 1
- 102220265732 rs1555059323 Human genes 0.000 description 1
- 102220340766 rs1555191832 Human genes 0.000 description 1
- 102220340219 rs1555191879 Human genes 0.000 description 1
- 102220268351 rs1555201383 Human genes 0.000 description 1
- 102220267786 rs1555281048 Human genes 0.000 description 1
- 102220268950 rs1555376567 Human genes 0.000 description 1
- 102220325921 rs1555376589 Human genes 0.000 description 1
- 102200085096 rs1555383892 Human genes 0.000 description 1
- 102200104507 rs1555524979 Human genes 0.000 description 1
- 102200106066 rs1555525970 Human genes 0.000 description 1
- 102220327839 rs1555567003 Human genes 0.000 description 1
- 102220282377 rs1555568338 Human genes 0.000 description 1
- 102220327404 rs1555568786 Human genes 0.000 description 1
- 102220271781 rs1555575857 Human genes 0.000 description 1
- 102220282620 rs1555594863 Human genes 0.000 description 1
- 102220288071 rs1555734984 Human genes 0.000 description 1
- 102220244717 rs1555803391 Human genes 0.000 description 1
- 102220276075 rs1555926784 Human genes 0.000 description 1
- 102220330782 rs1555926921 Human genes 0.000 description 1
- 102200018769 rs16991866 Human genes 0.000 description 1
- 102200042488 rs17883862 Human genes 0.000 description 1
- 102220097959 rs17883862 Human genes 0.000 description 1
- 102220276091 rs17883862 Human genes 0.000 description 1
- 102200164282 rs1799977 Human genes 0.000 description 1
- 102200149451 rs1800361 Human genes 0.000 description 1
- 102220003069 rs1800571 Human genes 0.000 description 1
- 102220074250 rs180177170 Human genes 0.000 description 1
- 102220032585 rs180177441 Human genes 0.000 description 1
- 102200079947 rs193302887 Human genes 0.000 description 1
- 102200079946 rs193302889 Human genes 0.000 description 1
- 102220008870 rs193922325 Human genes 0.000 description 1
- 102220211090 rs193922325 Human genes 0.000 description 1
- 102220024912 rs199472826 Human genes 0.000 description 1
- 102200097282 rs199472832 Human genes 0.000 description 1
- 102200097051 rs199473422 Human genes 0.000 description 1
- 102200097055 rs199473422 Human genes 0.000 description 1
- 102200097053 rs199473666 Human genes 0.000 description 1
- 102220113898 rs200246388 Human genes 0.000 description 1
- 102220173653 rs200563551 Human genes 0.000 description 1
- 102220046348 rs200632093 Human genes 0.000 description 1
- 102220161410 rs200900718 Human genes 0.000 description 1
- 102220280387 rs201054129 Human genes 0.000 description 1
- 102220058773 rs201103359 Human genes 0.000 description 1
- 102220036888 rs201676898 Human genes 0.000 description 1
- 102200108133 rs201753350 Human genes 0.000 description 1
- 102220097791 rs201753350 Human genes 0.000 description 1
- 102220218936 rs202004587 Human genes 0.000 description 1
- 102220007445 rs202088921 Human genes 0.000 description 1
- 102220058215 rs202168814 Human genes 0.000 description 1
- 102220062690 rs202208861 Human genes 0.000 description 1
- 102220227262 rs267601384 Human genes 0.000 description 1
- 102220011344 rs267606539 Human genes 0.000 description 1
- 102200164347 rs267607725 Human genes 0.000 description 1
- 102220036189 rs273585616 Human genes 0.000 description 1
- 102220005251 rs281864894 Human genes 0.000 description 1
- 102200060086 rs28363257 Human genes 0.000 description 1
- 102200060079 rs28363317 Human genes 0.000 description 1
- 102200115479 rs28933981 Human genes 0.000 description 1
- 102200095470 rs28940296 Human genes 0.000 description 1
- 102200057516 rs3218707 Human genes 0.000 description 1
- 102200118306 rs33919924 Human genes 0.000 description 1
- 102220005259 rs33921589 Human genes 0.000 description 1
- 102220005313 rs33921589 Human genes 0.000 description 1
- 102220005307 rs33922018 Human genes 0.000 description 1
- 102200118243 rs33937393 Human genes 0.000 description 1
- 102200118197 rs33946401 Human genes 0.000 description 1
- 102220005323 rs33946401 Human genes 0.000 description 1
- 102200118212 rs33950542 Human genes 0.000 description 1
- 102200118213 rs33950542 Human genes 0.000 description 1
- 102200117937 rs33957286 Human genes 0.000 description 1
- 102200118309 rs33961886 Human genes 0.000 description 1
- 102200118239 rs33966487 Human genes 0.000 description 1
- 102200118240 rs33966487 Human genes 0.000 description 1
- 102200118307 rs33969727 Human genes 0.000 description 1
- 102220005142 rs33971048 Human genes 0.000 description 1
- 102220005150 rs33971048 Human genes 0.000 description 1
- 102220005179 rs33971048 Human genes 0.000 description 1
- 102220005264 rs33974228 Human genes 0.000 description 1
- 102200117965 rs33983276 Human genes 0.000 description 1
- 102200117968 rs33983276 Human genes 0.000 description 1
- 102220005285 rs33983276 Human genes 0.000 description 1
- 102220005301 rs34139813 Human genes 0.000 description 1
- 102200082930 rs34378160 Human genes 0.000 description 1
- 102220005152 rs34439278 Human genes 0.000 description 1
- 102220243801 rs34515413 Human genes 0.000 description 1
- 102200127525 rs34635677 Human genes 0.000 description 1
- 102200082816 rs34868397 Human genes 0.000 description 1
- 102200118252 rs34933455 Human genes 0.000 description 1
- 102200118244 rs35209591 Human genes 0.000 description 1
- 102220082323 rs35269563 Human genes 0.000 description 1
- 102200117967 rs35461710 Human genes 0.000 description 1
- 102220041204 rs35962811 Human genes 0.000 description 1
- 102220251169 rs368061837 Human genes 0.000 description 1
- 102220286545 rs369999291 Human genes 0.000 description 1
- 102220177156 rs371431357 Human genes 0.000 description 1
- 102220242543 rs371782465 Human genes 0.000 description 1
- 102200108231 rs372201428 Human genes 0.000 description 1
- 102220067571 rs373957283 Human genes 0.000 description 1
- 102220058913 rs374396150 Human genes 0.000 description 1
- 102200049696 rs374550999 Human genes 0.000 description 1
- 102220341607 rs374625633 Human genes 0.000 description 1
- 102220250904 rs374984975 Human genes 0.000 description 1
- 102220211101 rs375173243 Human genes 0.000 description 1
- 102220343499 rs375493957 Human genes 0.000 description 1
- 102220280410 rs376797001 Human genes 0.000 description 1
- 102220248108 rs377129682 Human genes 0.000 description 1
- 102200091203 rs377715747 Human genes 0.000 description 1
- 102220187197 rs3809694 Human genes 0.000 description 1
- 102200067525 rs397507873 Human genes 0.000 description 1
- 102220013614 rs397516692 Human genes 0.000 description 1
- 102200122321 rs398124647 Human genes 0.000 description 1
- 102200163546 rs41295280 Human genes 0.000 description 1
- 102220085809 rs5030823 Human genes 0.000 description 1
- 102200091218 rs5030824 Human genes 0.000 description 1
- 102220266392 rs541477171 Human genes 0.000 description 1
- 102220229125 rs563306322 Human genes 0.000 description 1
- 102220223051 rs567617762 Human genes 0.000 description 1
- 102220212717 rs570325845 Human genes 0.000 description 1
- 102220277598 rs572906317 Human genes 0.000 description 1
- 102200020831 rs587776978 Human genes 0.000 description 1
- 102200153447 rs587777876 Human genes 0.000 description 1
- 102200153198 rs587777877 Human genes 0.000 description 1
- 102220039758 rs587778019 Human genes 0.000 description 1
- 102220040539 rs587778365 Human genes 0.000 description 1
- 102220027481 rs587779102 Human genes 0.000 description 1
- 102200106021 rs587780071 Human genes 0.000 description 1
- 102220036835 rs587780077 Human genes 0.000 description 1
- 102220088425 rs587780077 Human genes 0.000 description 1
- 102220041786 rs587780740 Human genes 0.000 description 1
- 102220041794 rs587780746 Human genes 0.000 description 1
- 102220044533 rs587781377 Human genes 0.000 description 1
- 102220060043 rs587781741 Human genes 0.000 description 1
- 102220045473 rs587782137 Human genes 0.000 description 1
- 102220060545 rs587782137 Human genes 0.000 description 1
- 102220045508 rs587782165 Human genes 0.000 description 1
- 102220045546 rs587782193 Human genes 0.000 description 1
- 102200062452 rs587782316 Human genes 0.000 description 1
- 102220045812 rs587782406 Human genes 0.000 description 1
- 102220046242 rs587782759 Human genes 0.000 description 1
- 102220046298 rs587782813 Human genes 0.000 description 1
- 102220330649 rs587782813 Human genes 0.000 description 1
- 102220049856 rs587783581 Human genes 0.000 description 1
- 102200110488 rs61748526 Human genes 0.000 description 1
- 102200110487 rs61748527 Human genes 0.000 description 1
- 102200110494 rs61748529 Human genes 0.000 description 1
- 102200110485 rs61751412 Human genes 0.000 description 1
- 102220044402 rs61756469 Human genes 0.000 description 1
- 102200110491 rs62654395 Human genes 0.000 description 1
- 102220027139 rs63750514 Human genes 0.000 description 1
- 102200164323 rs63750891 Human genes 0.000 description 1
- 102200101932 rs66741318 Human genes 0.000 description 1
- 102220032076 rs67016166 Human genes 0.000 description 1
- 102220032077 rs67016166 Human genes 0.000 description 1
- 102200101884 rs67294955 Human genes 0.000 description 1
- 102200101934 rs67954347 Human genes 0.000 description 1
- 102220032084 rs67954347 Human genes 0.000 description 1
- 102220037046 rs72552323 Human genes 0.000 description 1
- 102220032030 rs72554328 Human genes 0.000 description 1
- 102220032073 rs72556261 Human genes 0.000 description 1
- 102200101933 rs72556269 Human genes 0.000 description 1
- 102220032088 rs72556273 Human genes 0.000 description 1
- 102220032113 rs72558421 Human genes 0.000 description 1
- 102200101652 rs72558425 Human genes 0.000 description 1
- 102220032116 rs72558426 Human genes 0.000 description 1
- 102220032123 rs72558429 Human genes 0.000 description 1
- 102220052086 rs727503359 Human genes 0.000 description 1
- 102200013377 rs730880259 Human genes 0.000 description 1
- 102220011099 rs730881019 Human genes 0.000 description 1
- 102220058101 rs730881654 Human genes 0.000 description 1
- 102220058103 rs730881660 Human genes 0.000 description 1
- 102220087249 rs730881660 Human genes 0.000 description 1
- 102220057403 rs730881761 Human genes 0.000 description 1
- 102220057450 rs730881811 Human genes 0.000 description 1
- 102220286181 rs730881811 Human genes 0.000 description 1
- 102220057648 rs730881915 Human genes 0.000 description 1
- 102220195966 rs745387614 Human genes 0.000 description 1
- 102220264526 rs745754359 Human genes 0.000 description 1
- 102220062459 rs745943179 Human genes 0.000 description 1
- 102220286362 rs746206527 Human genes 0.000 description 1
- 102200106690 rs746504075 Human genes 0.000 description 1
- 102220286182 rs746624223 Human genes 0.000 description 1
- 102220239452 rs748145117 Human genes 0.000 description 1
- 102220153589 rs748395950 Human genes 0.000 description 1
- 102220331835 rs748609599 Human genes 0.000 description 1
- 102220093316 rs749037674 Human genes 0.000 description 1
- 102220059221 rs749091984 Human genes 0.000 description 1
- 102220339788 rs749180806 Human genes 0.000 description 1
- 102220313176 rs749459367 Human genes 0.000 description 1
- 102220083034 rs750032094 Human genes 0.000 description 1
- 102220061204 rs751176316 Human genes 0.000 description 1
- 102220059247 rs751628735 Human genes 0.000 description 1
- 102220156712 rs751722805 Human genes 0.000 description 1
- 102220093878 rs751914222 Human genes 0.000 description 1
- 102220317886 rs752416481 Human genes 0.000 description 1
- 102220203056 rs753524431 Human genes 0.000 description 1
- 102200121588 rs754036398 Human genes 0.000 description 1
- 102220094746 rs755022536 Human genes 0.000 description 1
- 102220260075 rs755022536 Human genes 0.000 description 1
- 102200147642 rs755402538 Human genes 0.000 description 1
- 102220265701 rs755705607 Human genes 0.000 description 1
- 102220104591 rs756750256 Human genes 0.000 description 1
- 102220227881 rs756750256 Human genes 0.000 description 1
- 102200153188 rs756928158 Human genes 0.000 description 1
- 102220153183 rs757900380 Human genes 0.000 description 1
- 102220277056 rs759501511 Human genes 0.000 description 1
- 102220277681 rs760158426 Human genes 0.000 description 1
- 102220271322 rs761562625 Human genes 0.000 description 1
- 102220327277 rs761562625 Human genes 0.000 description 1
- 102220095970 rs761681478 Human genes 0.000 description 1
- 102220316045 rs761767467 Human genes 0.000 description 1
- 102220264454 rs762630679 Human genes 0.000 description 1
- 102220257452 rs763104308 Human genes 0.000 description 1
- 102220218691 rs763269347 Human genes 0.000 description 1
- 102220086056 rs763707275 Human genes 0.000 description 1
- 102220286049 rs763707275 Human genes 0.000 description 1
- 102220222437 rs764367878 Human genes 0.000 description 1
- 102220285507 rs764565924 Human genes 0.000 description 1
- 102220316473 rs764755691 Human genes 0.000 description 1
- 102220270288 rs765472726 Human genes 0.000 description 1
- 102220276888 rs767140240 Human genes 0.000 description 1
- 102220058931 rs767371843 Human genes 0.000 description 1
- 102220194786 rs767371843 Human genes 0.000 description 1
- 102220032317 rs76914224 Human genes 0.000 description 1
- 102200006385 rs769382085 Human genes 0.000 description 1
- 102220322299 rs769382085 Human genes 0.000 description 1
- 102200115480 rs76992529 Human genes 0.000 description 1
- 102200081446 rs770546306 Human genes 0.000 description 1
- 102220215245 rs771612764 Human genes 0.000 description 1
- 102220242574 rs772196265 Human genes 0.000 description 1
- 102220062247 rs773918872 Human genes 0.000 description 1
- 102220083575 rs774156512 Human genes 0.000 description 1
- 102220218329 rs774156512 Human genes 0.000 description 1
- 102220059046 rs774162322 Human genes 0.000 description 1
- 102220277081 rs774162322 Human genes 0.000 description 1
- 102220277902 rs774964663 Human genes 0.000 description 1
- 102220145552 rs776421301 Human genes 0.000 description 1
- 102220282943 rs776471760 Human genes 0.000 description 1
- 102220257283 rs777351049 Human genes 0.000 description 1
- 102220105295 rs778284147 Human genes 0.000 description 1
- 102220153780 rs778473535 Human genes 0.000 description 1
- 102220462444 rs778785164 Human genes 0.000 description 1
- 102220259362 rs778943215 Human genes 0.000 description 1
- 102220242532 rs779472232 Human genes 0.000 description 1
- 102220214984 rs779514074 Human genes 0.000 description 1
- 102220086131 rs779664343 Human genes 0.000 description 1
- 102220276860 rs780241203 Human genes 0.000 description 1
- 102220222973 rs780588085 Human genes 0.000 description 1
- 102220281129 rs780865640 Human genes 0.000 description 1
- 102220201322 rs781725830 Human genes 0.000 description 1
- 102220266138 rs782308530 Human genes 0.000 description 1
- 102220276290 rs782343800 Human genes 0.000 description 1
- 102220062338 rs786201090 Human genes 0.000 description 1
- 102220061721 rs786202507 Human genes 0.000 description 1
- 102220060047 rs786202644 Human genes 0.000 description 1
- 102220059218 rs786203104 Human genes 0.000 description 1
- 102220061992 rs786203319 Human genes 0.000 description 1
- 102220059797 rs786203763 Human genes 0.000 description 1
- 102220265573 rs786204868 Human genes 0.000 description 1
- 102200070542 rs786205436 Human genes 0.000 description 1
- 102220271273 rs78957457 Human genes 0.000 description 1
- 102220067290 rs794727232 Human genes 0.000 description 1
- 102200151359 rs797045153 Human genes 0.000 description 1
- 102200070543 rs80338844 Human genes 0.000 description 1
- 102200070541 rs80338845 Human genes 0.000 description 1
- 102220021165 rs80357033 Human genes 0.000 description 1
- 102220021365 rs80357174 Human genes 0.000 description 1
- 102220020982 rs80357370 Human genes 0.000 description 1
- 102220117180 rs80357370 Human genes 0.000 description 1
- 102220021433 rs80357409 Human genes 0.000 description 1
- 102220009446 rs80357498 Human genes 0.000 description 1
- 102200058452 rs80358204 Human genes 0.000 description 1
- 102220018699 rs80358425 Human genes 0.000 description 1
- 102220018740 rs80358445 Human genes 0.000 description 1
- 102220019356 rs80358761 Human genes 0.000 description 1
- 102220082228 rs863224038 Human genes 0.000 description 1
- 102220083231 rs863224279 Human genes 0.000 description 1
- 102220086336 rs864622288 Human genes 0.000 description 1
- 102220087059 rs864622312 Human genes 0.000 description 1
- 102220086813 rs864622341 Human genes 0.000 description 1
- 102220086835 rs864622515 Human genes 0.000 description 1
- 102220089065 rs869312775 Human genes 0.000 description 1
- 102220089217 rs869312778 Human genes 0.000 description 1
- 102220093871 rs876658237 Human genes 0.000 description 1
- 102200108228 rs876658902 Human genes 0.000 description 1
- 102220097243 rs876659111 Human genes 0.000 description 1
- 102220094029 rs876659387 Human genes 0.000 description 1
- 102220094219 rs876659637 Human genes 0.000 description 1
- 102220097943 rs876659950 Human genes 0.000 description 1
- 102220283704 rs876659950 Human genes 0.000 description 1
- 102200105848 rs876660254 Human genes 0.000 description 1
- 102200105828 rs876660825 Human genes 0.000 description 1
- 102200105844 rs876660825 Human genes 0.000 description 1
- 102220094412 rs876660839 Human genes 0.000 description 1
- 102220099575 rs878853725 Human genes 0.000 description 1
- 102220099519 rs878853819 Human genes 0.000 description 1
- 102220099487 rs878854494 Human genes 0.000 description 1
- 102220103936 rs878854920 Human genes 0.000 description 1
- 102220104696 rs879253900 Human genes 0.000 description 1
- 102220104334 rs879254293 Human genes 0.000 description 1
- 102220105259 rs879254392 Human genes 0.000 description 1
- 102220105260 rs879254392 Human genes 0.000 description 1
- 102220105324 rs879254439 Human genes 0.000 description 1
- 102220105367 rs879254464 Human genes 0.000 description 1
- 102220105419 rs879254508 Human genes 0.000 description 1
- 102220105420 rs879254508 Human genes 0.000 description 1
- 102220105423 rs879254511 Human genes 0.000 description 1
- 102220105468 rs879254547 Human genes 0.000 description 1
- 102220105473 rs879254550 Human genes 0.000 description 1
- 102220197710 rs879254601 Human genes 0.000 description 1
- 102220105579 rs879254636 Human genes 0.000 description 1
- 102220113206 rs886038419 Human genes 0.000 description 1
- 102220115143 rs886039297 Human genes 0.000 description 1
- 102220118309 rs886041143 Human genes 0.000 description 1
- 102220292519 rs886041315 Human genes 0.000 description 1
- 102220119381 rs886042300 Human genes 0.000 description 1
- 102220121593 rs886042878 Human genes 0.000 description 1
- 102220131337 rs886045804 Human genes 0.000 description 1
- 102220170174 rs886052131 Human genes 0.000 description 1
- 102220186128 rs886053020 Human genes 0.000 description 1
- 102220191296 rs886056724 Human genes 0.000 description 1
- 102220141452 rs886057703 Human genes 0.000 description 1
- 102220263743 rs904809747 Human genes 0.000 description 1
- 102220222674 rs910355512 Human genes 0.000 description 1
- 102220214523 rs942019524 Human genes 0.000 description 1
- 102220214711 rs955371491 Human genes 0.000 description 1
- 102200045685 rs973321068 Human genes 0.000 description 1
- 102220217499 rs977251189 Human genes 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000003998 size exclusion chromatography high performance liquid chromatography Methods 0.000 description 1
- 239000011122 softwood Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 102220482187 tRNA pseudouridine synthase A_Y14S_mutation Human genes 0.000 description 1
- 230000010512 thermal transition Effects 0.000 description 1
- 101150080369 tpiA gene Proteins 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000003151 transfection method Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 230000010474 transient expression Effects 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000007160 ty medium Substances 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- 102220483148 von Hippel-Lindau disease tumor suppressor_L198R_mutation Human genes 0.000 description 1
- 102220504197 von Hippel-Lindau disease tumor suppressor_S68W_mutation Human genes 0.000 description 1
- 229910001868 water Inorganic materials 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 239000002759 woven fabric Substances 0.000 description 1
- 108010083879 xyloglucan endo(1-4)-beta-D-glucanase Proteins 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0083—Miscellaneous (1.14.99)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fodder In General (AREA)
- Detergent Compositions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (15)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361859704P | 2013-07-29 | 2013-07-29 | |
| US201361859712P | 2013-07-29 | 2013-07-29 | |
| US201361859666P | 2013-07-29 | 2013-07-29 | |
| US201361859721P | 2013-07-29 | 2013-07-29 | |
| US201361859630P | 2013-07-29 | 2013-07-29 | |
| US201361859735P | 2013-07-29 | 2013-07-29 | |
| US201361859680P | 2013-07-29 | 2013-07-29 | |
| US61/859,680 | 2013-07-29 | ||
| US61/859,666 | 2013-07-29 | ||
| US61/859,630 | 2013-07-29 | ||
| US61/859,704 | 2013-07-29 | ||
| US61/859,712 | 2013-07-29 | ||
| US61/859,735 | 2013-07-29 | ||
| US61/859,721 | 2013-07-29 | ||
| PCT/US2014/048075 WO2015017255A1 (en) | 2013-07-29 | 2014-07-24 | Variant enzymes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105683369A true CN105683369A (zh) | 2016-06-15 |
Family
ID=51299060
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201480052987.XA Pending CN105683369A (zh) | 2013-07-29 | 2014-07-24 | 变体酶 |
| CN201480053364.4A Pending CN105916981A (zh) | 2013-07-29 | 2014-07-24 | 变体酶 |
| CN201480052997.3A Pending CN105793417A (zh) | 2013-07-29 | 2014-07-24 | 变体酶 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201480053364.4A Pending CN105916981A (zh) | 2013-07-29 | 2014-07-24 | 变体酶 |
| CN201480052997.3A Pending CN105793417A (zh) | 2013-07-29 | 2014-07-24 | 变体酶 |
Country Status (10)
| Country | Link |
|---|---|
| US (4) | US10167460B2 (enExample) |
| EP (3) | EP3027742B1 (enExample) |
| JP (3) | JP2016533739A (enExample) |
| CN (3) | CN105683369A (enExample) |
| AU (3) | AU2014296573A1 (enExample) |
| BR (3) | BR112016002038A2 (enExample) |
| DK (2) | DK3027742T3 (enExample) |
| MX (3) | MX2016001319A (enExample) |
| WO (3) | WO2015017255A1 (enExample) |
| ZA (3) | ZA201600632B (enExample) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113913412A (zh) * | 2021-10-13 | 2022-01-11 | 湖北大学 | 蛋白酶k突变体及其制备方法 |
| CN116121434A (zh) * | 2022-09-26 | 2023-05-16 | 四川农业大学 | 一种控制小麦zip3a锌转运功能的氨基酸位点及分子标记 |
| CN117925577A (zh) * | 2024-03-19 | 2024-04-26 | 金堂佰信维科技有限公司 | 提高木聚糖酶活性的方法和木聚糖酶及其应用 |
| CN118910014A (zh) * | 2024-09-03 | 2024-11-08 | 广州青囊生物科技有限公司 | 一种生产稀有人参皂苷CK的β-葡萄糖苷酶突变体及其应用 |
| WO2025061210A3 (zh) * | 2023-09-19 | 2025-05-08 | 中国科学院天津工业生物技术研究所 | 一种嗜热内切纤维素酶突变体及制备方法 |
| CN120758467A (zh) * | 2025-09-11 | 2025-10-10 | 中国科学院天津工业生物技术研究所 | 裂解性多糖单加氧酶突变体及纤维素协同水解方法 |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2480660B1 (en) | 2009-09-23 | 2020-08-05 | Danisco US Inc. | Novel glycosyl hydrolase enzymes and uses thereof |
| CN107287250A (zh) | 2009-12-23 | 2017-10-24 | 丹尼斯科美国公司 | 提高同步糖化发酵反应效率的方法 |
| EP2686434B1 (en) | 2011-03-17 | 2021-07-14 | Danisco US Inc. | Method for reducing viscosity in saccharification process |
| EP3027742B1 (en) | 2013-07-29 | 2019-11-20 | Danisco US Inc. | Variant enzymes |
| WO2016138315A1 (en) | 2015-02-25 | 2016-09-01 | Danisco Us Inc | Alpha-glucosidase, compositions & methods |
| US10933121B2 (en) | 2015-09-02 | 2021-03-02 | Dupont Nutrition Biosciences Aps | Glycoside hydrolases and their use in preventing and/or treating a pathogenic infection in an animal |
| SG11201810998WA (en) * | 2016-06-15 | 2019-01-30 | Codexis Inc | Engineered beta-glucosidases and glucosylation methods |
| US20190330612A1 (en) | 2016-06-30 | 2019-10-31 | Danisco Us Inc | Aspartic proteases |
| WO2018053058A1 (en) | 2016-09-14 | 2018-03-22 | Danisco Us Inc. | Lignocellulosic biomass fermentation-based processes |
| RU2763378C2 (ru) | 2016-09-23 | 2021-12-28 | ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС | ПРИМЕНЕНИЕ АКТИВНЫХ ПРИ НИЗКОМ ЗНАЧЕНИИ pH АЛЬФА-1,4/1,6-ГЛИКОЗИДГИДРОЛАЗ В КАЧЕСТВЕ КОРМОВОЙ ДОБАВКИ ДЛЯ ЖВАЧНЫХ ЖИВОТНЫХ ДЛЯ УЛУЧШЕНИЯ ПЕРЕВАРИВАНИЯ КРАХМАЛА |
| WO2018106656A1 (en) * | 2016-12-06 | 2018-06-14 | Danisco Us Inc | Truncated lpmo enzymes and use thereof |
| WO2018112376A1 (en) | 2016-12-15 | 2018-06-21 | Danisco Us Inc. | Method for increasing the production of ethanol from corn fiber in a starch hydrolysis process |
| RU2771261C2 (ru) | 2016-12-21 | 2022-04-29 | ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС | Способы применения термостабильных сериновых протеаз |
| ES2680196B1 (es) * | 2017-02-01 | 2019-07-30 | Abengoa Bioenergia Nuevas Tecnologias Sa | Polipeptidos con actividad polisacarido monooxigenasa y su usopara la produccion de azucares fermentables |
| EP3592429A1 (en) | 2017-03-06 | 2020-01-15 | DuPont Nutrition Biosciences ApS | Novel fungal fucosidases and their use in preventing and/or treating a pathogenic infection in an animal |
| WO2019074828A1 (en) | 2017-10-09 | 2019-04-18 | Danisco Us Inc | CELLOBIOSE DEHYDROGENASE VARIANTS AND METHODS OF USE |
| CN111989400B (zh) | 2017-11-14 | 2024-05-24 | 丹尼斯科美国公司 | α-淀粉酶、组合物和方法 |
| CA3086023A1 (en) | 2017-12-19 | 2019-06-27 | Dupont Nutrition Biosciences Aps | Improved enzymatic modification of phospholipids in food |
| BR112021016727A2 (pt) | 2019-02-25 | 2021-11-03 | Dupont Nutrition Biosci Aps | Hidrolisados de proteína com rendimento aumentado de aminoácido n-terminal |
| CN110846294B (zh) * | 2019-11-29 | 2022-05-17 | 怀化学院 | 重组果胶酶及其基因、重组载体、制备方法和应用 |
| CN110846332B (zh) * | 2019-11-29 | 2022-05-17 | 怀化学院 | 一种果胶酶人工序列及其表达方法和应用 |
| CN110747212B (zh) * | 2019-11-29 | 2022-05-17 | 怀化学院 | 一种新果胶酶的基因及其蛋白表达、载体和应用 |
| CN111154743B (zh) * | 2020-04-01 | 2021-01-05 | 黑龙江省农业科学院畜牧兽医分院 | 突变型纤维素酶及其在玉米青贮中的应用 |
| WO2022266456A2 (en) | 2021-06-18 | 2022-12-22 | Dupont Nutrition Biosciences Aps | Proteases for beer haze reduction |
| CN114480146B (zh) * | 2022-04-15 | 2022-06-24 | 中国科学院天津工业生物技术研究所 | 一种米曲霉菌株在富集铜铁锰锌中的应用 |
| WO2024015974A1 (en) | 2022-07-15 | 2024-01-18 | Dupont Nutrition Biosciences Aps | Improved enzymatic modification of phospholipids in food |
| WO2024064653A1 (en) | 2022-09-20 | 2024-03-28 | Dupont Nutrition Biosciences Aps | Variant anti-viral polypeptides |
| WO2025049848A2 (en) | 2023-09-01 | 2025-03-06 | International N&H Denmark Aps | Proteases and aldc enzymes for beer haze and diacetyl reduction |
| CN120464599B (zh) * | 2025-07-11 | 2025-09-12 | 南京工业大学 | Pet水解酶突变体及其应用 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1662650A (zh) * | 2002-05-14 | 2005-08-31 | 诺和酶股份有限公司 | 果胶酸裂解酶变体 |
| CN102021159A (zh) * | 1997-10-23 | 2011-04-20 | 金克克国际有限公司 | 具有改变的净电荷、在去污剂中使用的多点置换的蛋白酶变体 |
| CN102333865A (zh) * | 2009-02-27 | 2012-01-25 | 埃欧金能源公司 | 新的木质素抗性纤维素酶 |
Family Cites Families (111)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1368599A (en) | 1970-09-29 | 1974-10-02 | Unilever Ltd | Softening compositions |
| GB1358599A (en) | 1970-12-22 | 1974-07-03 | Monacelli Umberto | Gun for firing tacks |
| DK187280A (da) | 1980-04-30 | 1981-10-31 | Novo Industri As | Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode |
| GB2095275B (en) | 1981-03-05 | 1985-08-07 | Kao Corp | Enzyme detergent composition |
| GB2094826B (en) | 1981-03-05 | 1985-06-12 | Kao Corp | Cellulase enzyme detergent composition |
| CA1338400C (en) | 1983-08-31 | 1996-06-18 | David H. Gelfand | Recombinant fungal cellulases |
| WO1989009259A1 (en) | 1988-03-24 | 1989-10-05 | Novo-Nordisk A/S | A cellulase preparation |
| US5648263A (en) | 1988-03-24 | 1997-07-15 | Novo Nordisk A/S | Methods for reducing the harshness of a cotton-containing fabric |
| DK115890D0 (da) | 1990-05-09 | 1990-05-09 | Novo Nordisk As | Enzym |
| US5246853A (en) | 1990-10-05 | 1993-09-21 | Genencor International, Inc. | Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I |
| US5475101A (en) | 1990-10-05 | 1995-12-12 | Genencor International, Inc. | DNA sequence encoding endoglucanase III cellulase |
| ES2170748T3 (es) | 1990-10-05 | 2002-08-16 | Genencor Int | Procedimientos para el tratamiento de tejidos que contienen algodon con celulasa. |
| FI932521A0 (fi) | 1993-06-02 | 1993-06-02 | Alko Ab Oy | Nytt endoglukanasenzym |
| US6268196B1 (en) | 1993-12-17 | 2001-07-31 | Genencor International, Inc. | Method and compositions for treating cellulose containing fabrics using truncated cellulase enzyme compositions |
| US5861271A (en) | 1993-12-17 | 1999-01-19 | Fowler; Timothy | Cellulase enzymes and systems for their expressions |
| US7005128B1 (en) | 1993-12-17 | 2006-02-28 | Genencor International, Inc. | Enzyme feed additive and animal feed including it |
| WO1997020025A1 (en) | 1995-11-27 | 1997-06-05 | Unilever N.V. | Enzymatic detergent compositions |
| TW552268B (en) | 1997-10-08 | 2003-09-11 | Yun-Ru Chen | Chimeric proteins with a cellulose binding domain (CBD) |
| JP2002176979A (ja) | 2000-12-12 | 2002-06-25 | Gencom Co | セルロース結合ドメインを細胞表層に提示する形質転換酵母 |
| US7045331B2 (en) | 2001-12-18 | 2006-05-16 | Genencor International, Inc. | EGVII endoglucanase and nucleic acids encoding the same |
| GB2389584A (en) | 2002-06-14 | 2003-12-17 | Norsk Hydro As | A fusion protein |
| WO2004016760A2 (en) * | 2002-08-16 | 2004-02-26 | Genencor International, Inc. | Novel variant hyprocrea jecorina cbh1 cellulases |
| US7273738B2 (en) * | 2002-10-01 | 2007-09-25 | Novozymes A/S | Family GH-61 polypeptides |
| US7387875B2 (en) | 2003-09-25 | 2008-06-17 | Novozymes, Inc. | Methods for monitoring multiple gene expression |
| CN1980953B (zh) | 2004-01-30 | 2014-12-24 | 诺维信股份有限公司 | 具有分解纤维增强活性的多肽及其编码多核苷酸 |
| BRPI0507431B1 (pt) | 2004-02-06 | 2021-07-27 | Novozymes, Inc | Célula hospedeira microbiana recombinante, construto de ácido nucleico, vetor de expressão recombinante, composição detergente, e, métodos para produzir o polipeptídeo gh61, para degradar um material celulósico e para produzir um produto de fermentação |
| US7413887B2 (en) | 2004-05-27 | 2008-08-19 | Genecor International, Inc. | Trichoderma reesei glucoamylase and homologs thereof |
| JP5149785B2 (ja) | 2005-04-12 | 2013-02-20 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | エタノールを得るためのバイオマスの処理 |
| US7781191B2 (en) | 2005-04-12 | 2010-08-24 | E. I. Du Pont De Nemours And Company | Treatment of biomass to obtain a target chemical |
| FR2885365B1 (fr) | 2005-05-04 | 2007-06-08 | Adisseo France Sas Soc Par Act | Gene abfb-2 de penicillium funiculosum |
| US7608689B2 (en) | 2005-09-30 | 2009-10-27 | Novozymes, Inc. | Methods for enhancing the degradation or conversion of cellulosic material |
| CN101421014B (zh) | 2006-02-13 | 2013-01-02 | 唐纳森公司 | 包括细纤维和反应、吸附或吸收颗粒的过滤网 |
| CA2698765A1 (en) | 2007-09-07 | 2009-03-19 | Danisco Us Inc. | Beta-glucosidase enhanced filamentous fungal whole cellulase compositions and methods of use |
| JP2009085831A (ja) | 2007-10-01 | 2009-04-23 | Canon Inc | 捕捉体部材及びバイオセンサー素子 |
| ES2504983T3 (es) | 2007-10-09 | 2014-10-09 | Danisco Us Inc. | Variantes de glucoamilasa |
| RU2378372C2 (ru) | 2008-03-03 | 2010-01-10 | ООО НПК "Фермтек" | Генетическая конструкция для обеспечения экспрессии целевых гомологичных и гетерологичных генов в клетках мицелиального гриба penicillium verruculosum, используемого в качестве хозяина, способ получения штамма гриба penicillium verruculosum и способ получения ферментного препарата |
| MY152105A (en) | 2008-03-21 | 2014-08-15 | Danisco Us Inc | Hemicellulase enriched compositions for enhancing hydrolysis of biomass |
| WO2009132008A2 (en) | 2008-04-21 | 2009-10-29 | Chromatin, Inc. | Methods and materials for processing a feedstock |
| BRPI0911966B1 (pt) | 2008-05-11 | 2018-11-13 | Univ Stellenbosch | polinucleotídeo codificando uma celobioidrolase |
| JP5507062B2 (ja) | 2008-06-16 | 2014-05-28 | 月桂冠株式会社 | 高発現プロモーター |
| CA2729945A1 (en) | 2008-07-07 | 2010-01-14 | Mascoma Corporation | Heterologous expression of termite cellulases in yeast |
| JP2010046034A (ja) * | 2008-08-22 | 2010-03-04 | Toyota Central R&D Labs Inc | 変異体のスクリーニング方法 |
| US8104512B2 (en) | 2008-09-25 | 2012-01-31 | Masco Corporation Of Indiana | Spout tip retention method |
| US8518684B2 (en) | 2008-11-18 | 2013-08-27 | Novozymes, Inc. | Methods and compositions for degrading cellulosic material |
| CN102292444A (zh) | 2008-11-20 | 2011-12-21 | 诺维信股份有限公司 | 具有淀粉分解增强活性的多肽和编码该多肽的多核苷酸 |
| US9506046B2 (en) | 2008-11-20 | 2016-11-29 | Novozymes, Inc. | Polypeptides having amylolytic enhancing activity and polynucleotides encoding same |
| EP2379732A2 (en) | 2008-12-19 | 2011-10-26 | Novozymes Inc. | Methods for increasing enzymatic hydrolysis of cellulosic material in the presence of a peroxidase |
| US8337663B2 (en) | 2008-12-19 | 2012-12-25 | Novozymes, Inc. | Methods for increasing hydrolysis of cellulosic material |
| BRPI0923026A2 (pt) | 2008-12-19 | 2017-03-28 | Novozymes Inc | métodos para degradar ou converter um material celulósico, para produzir um produto de fermentação, e para fermentar um material celulósico. |
| FI122028B (fi) * | 2008-12-30 | 2011-07-29 | Ab Enzymes Oy | Sieniperäiset endoglukanaasit, niiden tuotto ja käyttö |
| RU2560424C2 (ru) | 2009-02-20 | 2015-08-20 | ДАНИСКО ЮЭс ИНК. | Способ получения состава ферментационного бульона |
| WO2010138754A1 (en) | 2009-05-29 | 2010-12-02 | Novozymes, Inc. | Methods for enhancing the degradation or conversion of cellulosic material |
| CN102803482B (zh) * | 2009-06-03 | 2015-04-29 | 丹尼斯科美国公司 | 具有改善的表达、活性和/或稳定性的纤维素酶变体,及其用途 |
| BR112012000078A2 (pt) | 2009-07-03 | 2017-03-01 | Dsm Ip Assets Bv | cepas de talaromyces e composições de enzima. |
| MX2012002032A (es) * | 2009-08-19 | 2012-03-26 | Danisco | Variantes de glucoamilasa. |
| EP2480660B1 (en) | 2009-09-23 | 2020-08-05 | Danisco US Inc. | Novel glycosyl hydrolase enzymes and uses thereof |
| US20110086236A1 (en) | 2009-10-13 | 2011-04-14 | The Penn State Research Foundation | Composites containing polypeptides attached to polysaccharides and molecules |
| EP2599864B1 (en) | 2009-11-20 | 2017-08-02 | Danisco US Inc. | Beta-glucosidase variants with improved properties |
| MX2012006408A (es) | 2009-12-04 | 2012-09-07 | Georgia Tech Res Inst | Metodos mejorados para tratar biomasa por hidrolisis enzimatica. |
| CN107287250A (zh) | 2009-12-23 | 2017-10-24 | 丹尼斯科美国公司 | 提高同步糖化发酵反应效率的方法 |
| TWI433934B (zh) * | 2010-01-28 | 2014-04-11 | Academia Sinica | 新型β-葡萄糖苷酶及其用途 |
| WO2011097713A1 (en) | 2010-02-11 | 2011-08-18 | Iogen Energy Corporation | Carbohydrate binding modules with reduced binding to lignin |
| FR2957922A1 (fr) | 2010-03-26 | 2011-09-30 | Inst Francais Du Petrole | Nouvelles proteines de fusion cbh1-eg1 et leur utilisation |
| BR112012033404B1 (pt) | 2010-06-29 | 2021-05-18 | Dsm Ip Assets B.V. | célula recombinante, método para a preparação de um polipeptídeo, composição, método para o tratamento de um substrato que compreende material de carboidrato e uso de um polipeptídeo |
| DK2591119T4 (da) | 2010-07-07 | 2022-12-12 | Novozymes North America Inc | Fermenteringsproces |
| CN103314111A (zh) * | 2010-08-06 | 2013-09-18 | 诺维信公司 | 降解或水解多糖的方法 |
| US9458483B2 (en) | 2010-08-12 | 2016-10-04 | Novozymes, Inc. | Compositions comprising a polypeptide having cellulolytic enhancing activity and a bicyclic compound and uses thereof |
| JP5810489B2 (ja) | 2010-08-20 | 2015-11-11 | 株式会社豊田中央研究所 | セルラーゼ活性を有するタンパク質及びその利用 |
| EP2606131B1 (en) | 2010-08-20 | 2017-03-08 | Codexis, Inc. | Use of glycoside hydrolase 61 family proteins in processing of cellulose |
| US9410135B2 (en) * | 2010-09-15 | 2016-08-09 | The Regents Of The University Of California | Thermophilic mutants of Trichoderma reesei endoglucanase I |
| ES2594528T3 (es) | 2010-09-30 | 2016-12-20 | Novozymes, Inc. | Variantes de polipéptidos con actividad de mejora celulolítica y polinucleótidos que las codifican |
| PL2635671T3 (pl) | 2010-11-02 | 2017-02-28 | Codexis, Inc. | Ulepszone szczepy grzybowe |
| WO2012068509A1 (en) | 2010-11-18 | 2012-05-24 | Novozymes, Inc. | Chimeric polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| RU2013128619A (ru) | 2010-11-24 | 2014-12-27 | Новартис Интернэшнл Фармасьютикал Лтд. | Слитные ферменты |
| AU2011350442B2 (en) | 2010-12-28 | 2016-09-15 | Japan Tobacco Inc. | Modified tamavidin |
| US20130018172A1 (en) | 2011-01-13 | 2013-01-17 | Echogen, Inc. | Metal Abstraction Peptide and Uses Thereof |
| MX2013010510A (es) | 2011-03-17 | 2013-10-07 | Danisco Inc | Enzimas glocosil hidrolasa y usos de estas para la hidrolisis de biomasa. |
| EP2686434B1 (en) | 2011-03-17 | 2021-07-14 | Danisco US Inc. | Method for reducing viscosity in saccharification process |
| EP2686427A1 (en) | 2011-03-17 | 2014-01-22 | Danisco US Inc. | Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars |
| US20140080182A1 (en) | 2011-03-31 | 2014-03-20 | Novozymes, Inc. | Cellulose Binding Domain Variants and Polynucleotides Encoding Same |
| CN103814128A (zh) | 2011-04-04 | 2014-05-21 | 加利福尼亚大学董事会 | 增强的纤维素降解 |
| CA2835606A1 (en) | 2011-04-07 | 2012-10-11 | Synaptic Research, Llc | Single chain antibodies for photosynthetic microorganisms and methods of use |
| CN103797126A (zh) | 2011-04-29 | 2014-05-14 | 诺维信股份有限公司 | 用于增强纤维素材料的降解或转化的方法 |
| BR112014004171A2 (pt) * | 2011-08-22 | 2018-11-06 | Codexis Inc | variantes da proteína glicósideo hidrolase gh61 e cofatores que aumentam a atividade de gh61 |
| US9663772B2 (en) | 2011-08-24 | 2017-05-30 | Novozymes, Inc. | Aspergillus fumigatus cellulolytic enzyme compositions and uses thereof |
| US9279163B2 (en) | 2011-08-31 | 2016-03-08 | Iogen Energy Corporation | Cellobiohydrolase enzymes |
| PL2756078T3 (pl) | 2011-09-14 | 2017-12-29 | Dupont Nutrition Biosciences Aps | Kompozycje zawierające enzymy o aktywności endo-1,4-beta-ksylanazy i enzymy o aktywności endo-1, 3(4)-beta glukanazy |
| EP3219794B1 (en) | 2011-11-21 | 2019-09-11 | Novozymes A/S | Gh61 polypeptide variants and polynucleotides encoding same |
| WO2013110242A1 (en) | 2012-01-29 | 2013-08-01 | Novozymes A/S | Methods for degrading or converting cellulosic material |
| EP2839011B1 (en) | 2012-04-17 | 2016-09-14 | F. Hoffmann-La Roche AG | Method for the expression of polypeptides using modified nucleic acids |
| EP2838989A4 (en) | 2012-04-19 | 2015-12-09 | Dyadic Internat Usa Inc | METHOD FOR INCREASING THE EFFECT OF CELLULASEENY SYMMBOLS IN THE SUGARING OF A (LIGNO) CELLULOSE MATERIAL |
| CN113234695A (zh) | 2012-04-27 | 2021-08-10 | 诺维信股份有限公司 | Gh61多肽变体以及编码其的多核苷酸 |
| WO2013177714A1 (en) | 2012-05-31 | 2013-12-05 | Iogen Energy Corporation | Cellulose-degrading enzyme composition comprising gh16 |
| AU2012387042A1 (en) | 2012-08-03 | 2015-02-19 | Dupont Nutrition Biosciences Aps | Enzymes |
| WO2014092832A2 (en) | 2012-09-19 | 2014-06-19 | Novozymes, Inc. | Methods for enhancing the degradation or conversion of cellulosic material |
| US20150307562A1 (en) | 2012-11-20 | 2015-10-29 | Pronutria Biosciences, Inc. | Engineered secreted proteins and methods |
| CN103966252A (zh) | 2013-01-28 | 2014-08-06 | 北大工学院绍兴技术研究院 | 纤维素结合域在棒状杆菌分泌表达系统中的应用 |
| CN105074001A (zh) | 2013-02-21 | 2015-11-18 | 诺维信公司 | 糖化和发酵纤维素材料的方法 |
| CN105517450A (zh) | 2013-06-21 | 2016-04-20 | 杜邦营养生物科学有限公司 | 提高木质纤维素生物质营养价值的方法和组合物 |
| EP3010352B1 (en) | 2013-06-21 | 2020-12-02 | DuPont Nutrition Biosciences ApS | Method of preparing feed additive |
| WO2015004098A1 (en) | 2013-07-11 | 2015-01-15 | Dsm Ip Assets B.V. | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
| EP3027742B1 (en) | 2013-07-29 | 2019-11-20 | Danisco US Inc. | Variant enzymes |
| DK3052620T3 (da) | 2013-09-04 | 2020-09-07 | Novozymes As | Fremgangsmåder til forøgelse af enzymatisk hydrolyse af celluloseholdigt materiale |
| WO2015048334A2 (en) | 2013-09-25 | 2015-04-02 | Pronutria, Inc. | Edible species nutritive polypeptides and methods of production and use thereof |
| DK3092312T3 (en) | 2014-01-07 | 2019-03-04 | Novozymes As | INSURANCE OF MAN-MADE CELLULOSE-CONTAINING MATERIALS |
| KR20230155591A (ko) | 2014-04-30 | 2023-11-10 | 베르살리스 에스.피.에이. | 리그노셀룰로스 물질을 효소적으로 가수분해하고 당을 발효시키는 방법 |
| WO2015175308A1 (en) | 2014-05-13 | 2015-11-19 | Danisco Us Inc. | Improved enzymatic hydrolysis of biomass |
| MX383227B (es) | 2014-05-14 | 2025-03-13 | UNIV AUTòNOMA DE BARCELONA | Vectores de virus adenoasociados para el tratamiento de trastornos de almacenamiento lisosomico. |
| US20170233746A1 (en) | 2014-08-15 | 2017-08-17 | Danisco Us Inc. | Compositions and methods for improved protein production |
| EP3191597A1 (en) | 2014-08-21 | 2017-07-19 | Novozymes A/S | Process for saccharifying cellulosic material under oxygen addition |
| EP2993230A1 (en) | 2014-09-02 | 2016-03-09 | Clariant Produkte (Deutschland) GmbH | Polypeptides having xylanase activity with a high conversion rate of xylose-containing polysaccharides |
-
2014
- 2014-07-24 EP EP14748456.2A patent/EP3027742B1/en active Active
- 2014-07-24 CN CN201480052987.XA patent/CN105683369A/zh active Pending
- 2014-07-24 MX MX2016001319A patent/MX2016001319A/es unknown
- 2014-07-24 BR BR112016002038A patent/BR112016002038A2/pt not_active Application Discontinuation
- 2014-07-24 AU AU2014296573A patent/AU2014296573A1/en not_active Abandoned
- 2014-07-24 US US14/909,031 patent/US10167460B2/en active Active
- 2014-07-24 WO PCT/US2014/048075 patent/WO2015017255A1/en not_active Ceased
- 2014-07-24 WO PCT/US2014/048077 patent/WO2015017256A1/en not_active Ceased
- 2014-07-24 JP JP2016531773A patent/JP2016533739A/ja active Pending
- 2014-07-24 EP EP14750877.4A patent/EP3027743A1/en not_active Withdrawn
- 2014-07-24 BR BR112016002067A patent/BR112016002067A2/pt not_active Application Discontinuation
- 2014-07-24 BR BR112016002045A patent/BR112016002045A2/pt not_active IP Right Cessation
- 2014-07-24 MX MX2016001315A patent/MX2016001315A/es unknown
- 2014-07-24 EP EP14748455.4A patent/EP3027741B1/en active Active
- 2014-07-24 JP JP2016531775A patent/JP2016533741A/ja active Pending
- 2014-07-24 CN CN201480053364.4A patent/CN105916981A/zh active Pending
- 2014-07-24 US US14/909,007 patent/US10081802B2/en not_active Expired - Fee Related
- 2014-07-24 JP JP2016531774A patent/JP2016533740A/ja active Pending
- 2014-07-24 DK DK14748456.2T patent/DK3027742T3/da active
- 2014-07-24 MX MX2016001317A patent/MX2016001317A/es unknown
- 2014-07-24 DK DK14748455.4T patent/DK3027741T3/da active
- 2014-07-24 CN CN201480052997.3A patent/CN105793417A/zh active Pending
- 2014-07-24 AU AU2014296574A patent/AU2014296574A1/en not_active Abandoned
- 2014-07-24 US US14/909,041 patent/US10479983B2/en not_active Expired - Fee Related
- 2014-07-24 AU AU2014296572A patent/AU2014296572A1/en not_active Abandoned
- 2014-07-24 WO PCT/US2014/048067 patent/WO2015017254A1/en not_active Ceased
-
2016
- 2016-01-28 ZA ZA2016/00632A patent/ZA201600632B/en unknown
- 2016-01-28 ZA ZA2016/00633A patent/ZA201600633B/en unknown
- 2016-01-28 ZA ZA2016/00631A patent/ZA201600631B/en unknown
-
2018
- 2018-11-09 US US16/185,619 patent/US20190169587A1/en not_active Abandoned
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102021159A (zh) * | 1997-10-23 | 2011-04-20 | 金克克国际有限公司 | 具有改变的净电荷、在去污剂中使用的多点置换的蛋白酶变体 |
| CN1662650A (zh) * | 2002-05-14 | 2005-08-31 | 诺和酶股份有限公司 | 果胶酸裂解酶变体 |
| CN102333865A (zh) * | 2009-02-27 | 2012-01-25 | 埃欧金能源公司 | 新的木质素抗性纤维素酶 |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113913412A (zh) * | 2021-10-13 | 2022-01-11 | 湖北大学 | 蛋白酶k突变体及其制备方法 |
| CN113913412B (zh) * | 2021-10-13 | 2023-10-03 | 湖北大学 | 蛋白酶k突变体及其制备方法 |
| CN116121434A (zh) * | 2022-09-26 | 2023-05-16 | 四川农业大学 | 一种控制小麦zip3a锌转运功能的氨基酸位点及分子标记 |
| CN116121434B (zh) * | 2022-09-26 | 2023-12-12 | 四川农业大学 | 一种控制小麦zip3a锌转运功能的氨基酸位点及分子标记 |
| WO2025061210A3 (zh) * | 2023-09-19 | 2025-05-08 | 中国科学院天津工业生物技术研究所 | 一种嗜热内切纤维素酶突变体及制备方法 |
| CN117925577A (zh) * | 2024-03-19 | 2024-04-26 | 金堂佰信维科技有限公司 | 提高木聚糖酶活性的方法和木聚糖酶及其应用 |
| CN117925577B (zh) * | 2024-03-19 | 2024-06-14 | 金堂佰信维科技有限公司 | 提高木聚糖酶活性的方法和木聚糖酶及其应用 |
| CN118910014A (zh) * | 2024-09-03 | 2024-11-08 | 广州青囊生物科技有限公司 | 一种生产稀有人参皂苷CK的β-葡萄糖苷酶突变体及其应用 |
| CN120758467A (zh) * | 2025-09-11 | 2025-10-10 | 中国科学院天津工业生物技术研究所 | 裂解性多糖单加氧酶突变体及纤维素协同水解方法 |
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP3027741B1 (en) | Gh61 enzyme variants | |
| US9523087B2 (en) | Cellulase variants with improved expression, activity and stability, and use thereof | |
| CN104870644A (zh) | 纤维二糖水解酶的变体 | |
| US20150315560A1 (en) | Variants of cellobiohydrolases | |
| US20160122735A1 (en) | Variants of cellobiohydrolases | |
| US20160272959A1 (en) | Variants of cellobiohydrolases | |
| CN104870638A (zh) | 纤维二糖水解酶的变体 | |
| Class et al. | Patent application title: VARIANTS OF CELLOBIOHYDROLASES |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160615 |