CN105617316A

CN105617316A - Antibacterial disinfectant for clinical laboratory and preparation method

Info

Publication number: CN105617316A
Application number: CN201610000231.1A
Authority: CN
Inventors: 韩凤霞; 高建红; 周卫岭
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-01-02
Filing date: 2016-01-02
Publication date: 2016-06-01

Abstract

The invention relates to an antibacterial disinfectant for a clinical laboratory and a preparation method, and belongs to the field of traditional Chinese medicine. The active ingredients of the disinfectant comprise the following raw materials: pinkflower ecdysanthera roots and leaves, henry hemsleya roots, knotgrass, ovate catalpa leaves, grossedentate clematis stems and leaves, spinach beet, phellandrium, rhizoma zingiberis, disinfectant, camphortree leaves, moutan barks, garlics, alisma orientale, common jujube leaves, wood sorrel and spina gleditsiae. Medical materials selected by the disinfectant are properly matched; the medicine conforms to the traditional Chinese medicine and modern medicine theory; the disinfectant has the characteristics of heat and toxic material clearing effects, antibacterial and sterilization functions, insect killing and inflammation diminishing functions, water solubility, high stability and the like; in addition, the disinfectant has no stimulation on the skin of a human body; the stability is high; the disinfectant is safe and has no toxic and side effects, and can be widely applied to the disinfection of the skin of personnel in the clinical laboratory and the indoor environment; the work efficiency of the personnel in the clinical laboratory is improved; the accuracy rate of the inspection result is ensured; the disinfectant belongs to an ideal antibacterial disinfectant worthy of being popularized.

Description

One clinical laboratory antibacterial disinfectant solution and preparation method

Technical field

The present invention relates to the field of Chinese medicines, particularly to one clinical laboratory antibacterial disinfectant solution and preparation method.

Background technology

Clinical laboratory is the bridge between clinical medicine and preclinical medicine, the subdiscipline such as including clinical chemistry, clinical microbiology, clinical immunology, hematology, osology and transfusion science, undertakes the detection work of various human bodies and the zoological specimens including ward, Emergency call patient, all kinds of health check-up and scientific research every day.

Clinical laboratory is the place that pathogenic microorganism compares concentration, it has concentrated the specimen such as the human body fluid of clinical departments room censorship, blood, respiratory secretions, gastrointestinal tract Excreta, being the key departments of Infection Prevention and control, reviewer is also one of emphasis personnel of monitoring of hospital infection. Therefore, the security protection of clinical laboratory staff is strengthened and the strict sterilization of environment is extremely important.

In prior art, the sterilization sterilizing of clinical laboratory's interior space, conventional method has chemical reagent sterilizing, ray sterilizing, dry heat sterilization, warm sterilizing and filtration sterilization and germ killing drugs suffocating sterilization. Although these a few class disinfection way play certain disinfective action, but thorough, pungent of easily sterilizing, the skin of easy damaged reviewer, instability, even can only play the effect purifying air, do not possess the effect to body-care, have a strong impact on the respiratory system of clinical laboratory personnel and the accuracy of sample survey result.

Therefore clinical laboratory with antibacterial disinfectant solution must safely, effectively, have no side effect, and Chinese materia medica is as the traditional medicine of China, there is the advantages such as effect strong, instant effect, lasting medicine, and there is no dependency, drug resistance and toxic and side effects, soluble in water, stability is high, therefore the antibacterial disinfectant solution of selection check section, for checking the environment tool of section office to be of great significance.

Summary of the invention

The invention provides one clinical laboratory antibacterial disinfectant solution and preparation method; Selected by disinfectant solution of the present invention, medical material compatibility is suitable, meet Chinese medicine and modern medicine is theoretical, there is heat-clearing and toxic substances removing, bacteriostasis and sterilization, parasite killing antiinflammatory, soluble in water, stability high, and human body skin is non-stimulated, stability is high, safe without toxic side effect, can be widely applied to clinical laboratory's personnel's skin and the sterilization to indoor environment, improve the work of clinical laboratory personnel

Efficiency, it is ensured that the accuracy rate of assay, is a kind of antibacterial disinfectant solution of the ideal being worthy to be popularized.

For achieving the above object, the enforcement of technical scheme is as follows:

The antibacterial disinfectant solution of a kind of clinical laboratory, makes the raw material of described disinfectant solution effective ingredient and forms and parts by weight are:

Radix Seu Folium Ecdysantherae Roseae 55-85 part Neoalsomitra integrifoliola (Cogn.) Hucth. 55-85 part Herba Polygoni Avicularis 55-85 part Folium catalpae ovatae 52-80 part

Herba Clematidis Buchananianae 52-80 part dish 52-80 part Herba Limnophilae rugosae 47-75 part Rhizoma Zingiberis 45-75 part

Disinfectant 40-68 part Folium cinnamomi camphorae 40-68 part Cortex Moutan 40-68 part Bulbus Allii 35-62 part

Rhizoma Alismatis 35-62 part Folium Jujubae 35-62 part Herba Oxalidis Corniculatae 30-55 part Spina Gleditsiae 25-45 part.

Make described disinfectant solution effective ingredient raw material composition and parts by weight be:

76 parts of Neoalsomitra integrifoliola (Cogn.) Hucth.s of Radix Seu Folium Ecdysantherae Roseae, 76 parts of Folium catalpae ovatae of 76 parts of Herba Polygoni Avicularis 72 parts

72 portions of dishes of Herba Clematidis Buchananianae, 65 portions of Rhizoma Zingiberiss of 72 portions of Herba Limnophilae rugosaes 65 parts

53 parts of 58 parts of Folium cinnamomi camphorae of disinfectant, 58 portions of Bulbus Alliis of 58 parts of Cortex Moutans

53 parts of Folium Jujubae of Rhizoma Alismatis, 45 parts of Spina Gleditsiaes of 53 parts of Herba Oxalidis Corniculataes 38 parts.

69 parts of Neoalsomitra integrifoliola (Cogn.) Hucth.s of Radix Seu Folium Ecdysantherae Roseae, 69 parts of Folium catalpae ovatae of 69 parts of Herba Polygoni Avicularis 64 parts

64 portions of dishes of Herba Clematidis Buchananianae, 59 portions of Rhizoma Zingiberiss of 64 portions of Herba Limnophilae rugosaes 59 parts

47 parts of 52 parts of Folium cinnamomi camphorae of disinfectant, 52 portions of Bulbus Alliis of 52 parts of Cortex Moutans

47 parts of Folium Jujubae of Rhizoma Alismatis, 40 parts of Spina Gleditsiaes of 47 parts of Herba Oxalidis Corniculataes 34 parts.

62 parts of Neoalsomitra integrifoliola (Cogn.) Hucth.s of Radix Seu Folium Ecdysantherae Roseae, 62 parts of Folium catalpae ovatae of 62 parts of Herba Polygoni Avicularis 58 parts

58 portions of dishes of Herba Clematidis Buchananianae, 53 portions of Rhizoma Zingiberiss of 58 portions of Herba Limnophilae rugosaes 53 parts

41 parts of 46 parts of Folium cinnamomi camphorae of disinfectant, 46 portions of Bulbus Alliis of 46 parts of Cortex Moutans

46 parts of Folium Jujubae of Rhizoma Alismatis, 46 parts of Spina Gleditsiaes of 46 parts of Herba Oxalidis Corniculataes 29 parts.

A kind of clinical laboratory preparation method of antibacterial disinfectant solution, comprises the following steps that:

(1) Radix Seu Folium Ecdysantherae Roseae, Herba Clematidis Buchananianae, Herba Limnophilae rugosae, Rhizoma Zingiberis, disinfectant, Bulbus Allii and Spina Gleditsiae decontamination are cleaned, are placed in container, add the distilled water immersion of 8-10 times amount after 4-6 hour, boil 3-4 hour,

Extract; Again add 5-7 times amount distilled water, boil 2-3 hour, extract; Finally, add 2-4 times amount distilled water, heated and boiled 1-2 hour, extract; Merge three extracting solution, filter, obtain filtrate, standby;

(2) Neoalsomitra integrifoliola (Cogn.) Hucth., Cortex Moutan and Rhizoma Alismatis are cleaned, vinegar is added wherein, mixes thoroughly, vexed, take out stripping and slicing, sun dehydration, be placed in mortar, obtained the fine powder of 160 orders, standby;

(3) by Herba Polygoni Avicularis and dish decontamination, clean, dry, be placed in mortar, grind into powder, obtained the fine powder of 180 orders, standby;

(4) by Folium catalpae ovatae, Folium cinnamomi camphorae, Folium Jujubae and Herba Oxalidis Corniculatae decontamination, clean, be placed in container, add 5 times amount 80% alcohol solution dipping 2-3 hour, after heating extraction 5-6 hour, reclaim ethanol, filter, obtain filtrate, standby;

(5) distilled water of 10-12 times amount is added with the fine powder of step (2), step (3) gained after step (1), the filtrate of step (4) gained being mixed, after stirring and dissolving is uniform, heated and boiled 0.5 hour, is placed in less than 60 DEG C, stands 1 hour, filter, filtrate, filtrate fill is sealed up for safekeeping, 121 DEG C of pressure sterilizings 20 minutes, packaging, is the antibacterial disinfectant solution of described clinical laboratory.

The property of medicine of Chinese medicine used by the present invention is as follows:

Radix Seu Folium Ecdysantherae Roseae: sour in the mouth, property is put down; Return Liver Channel. Heat-clearing and toxic substances removing, dampness removingization is stagnant, promoting blood circulation and detumescence. For laryngopharynx swelling and pain, stomatitis, enteritis, chronic nephritis, dyspepsia distension, carbuncle sore tumefacting virus, rheumatic arthralgia, treating swelling and pain by traumatic injury. " Luchuan book on Chinese herbal medicine " load: " antiinflammatory, sterilization, removing the necrotic tissue is dirty. Control stomatitis, laryngitis, gingivitis, furuncle ulcer. Solve big tea poison of drug, poison eaten by wood, Fructus Crotonis poison. "

Neoalsomitra integrifoliola (Cogn.) Hucth.: bitter in the mouth, puckery, cold in nature. Heat clearing away, removing toxic substances, pain relieving. For bacillary dysentery enteritis, gastritis pharyngolaryngitis, parotitis, periodontitis, epidemic hemorrhagic conjunctivitis, tonsillitis, urinary tract infection. " choosing of Simao, Yunnan Chinese herbal medicine " carries: " heat-clearing and toxic substances removing, anti-inflammation and sterilization restrain pain relieving. Control bacillary dysentery, acute gastroenteritis, gastric and duodenal ulcers, abdominal pain, diarrhoea, urinary tract infection, high heat, pharyngolaryngitis, tonsillitis, have blood in stool, neurasthenia. "

Herba Polygoni Avicularis: bitter in the mouth, cold nature; Return bladder warp. Inducing diuresis for treating stranguria syndrome, killing parasites for relieving itching. For bladder pyretic stranguria, oliguria with reddish urine, drench the puckery pain of drop, skin eczema, pudendal pruritus leukorrhagia. " herbal classic " carries: " main contamination, scabies scabies cellulitis hemorrhoid kill three worms. " modern medicine study confirmation, fungus, antibacterial are had inhibitory action by Herba Polygoni Avicularis.

Folium catalpae ovatae: bitter in the mouth, cold in nature; GUIXIN, lung meridian. Heat-clearing and toxic substances removing, killing parasites for relieving itching. For fever of children, skin ulcer

Furuncle scabies. Modern medicine study confirms, staphylococcus aureus, escherichia coli, aerobacteria are had inhibitory action by Folium catalpae ovatae cooling bath, warm macerating liquid.

Herba Clematidis Buchananianae: sweet in the mouth, pungent, bitter, cool in nature. Antiinflammatory, diuresis, pain relieving. For tonsillitis, pharyngolaryngitis, urethritis, cystitis, traumatic injury. " Traditional Chinese Medicine in Sichuan will " carries: " parasite killing detoxifies. Controlling aphonia hoarseness, syphilitic sore, worm skin ulcer is rotten for a long time and difficult labour is grown wild. "

Dish: sweet in the mouth, hardship, cold in nature; Return lung, kidney, large intestine channel. Heat-clearing and toxic substances removing, clots absorbing stops blooding. For time row calentura, hemorrhoid, measles without adequate eruption, spit blood, pyretic toxicity dysentery, amenorrhea stranguria with turbid discharge, traumatic injury, snakeworm hinder. " occupying diet spectrum with breath " load: " relieving inflammation or internal heat and dispel the wind, parasite killing detoxifies, and washs dirt turbid, and rare variola, leukorrhagia stopping regulating menstruation, treating stranguria dysentery of controlling, children's married woman You Yi eats it. "

Herba Limnophilae rugosae: acrid in the mouth, property is put down; Return lung, spleen, stomach warp. Clearing away heat and expelling pathogen in the exterior, expelling wind and removing dampness, cough-relieving pain relieving. For catching a cold, laryngopharynx swelling and pain, cough due to lung-heat, bronchitis, stomachache, pemphigus. " Nanning City's medicine will " carries: " dampness removes expectorant, reducing swelling and alleviating pain, and parasite killing detoxifies. Control asthma and cough, children's's milk abdominal mass, furuncle, snakebite, cold syndrome of the stomach pain. "

Rhizoma Zingiberis: acrid in the mouth, hot in nature; Return spleen, stomach, kidney, the heart, lung meridian. Warming middle-JIAO is by cold, and recuperating depleted YANG is promoted blood circulation. For trusted subordinate's cold type of pain, vomit and diarrhoea, cold extremities faint pulse, breath with cough for cold time, anemofrigid-damp arthralgia. " not Lu " load: " control abdominal pain due to cold, attacked by pestiferous factors, cholera, distension, all poison of ailment said due to cold or exposure, stagnation of QI between skin, only hemoptysis. " modern medicine study confirmation, the ether extract of Rhizoma Zingiberis and water extract all have obvious antiinflammatory action.

Disinfectant: mildly bitter flavor, cool in nature. Heat-clearing and toxic substances removing, cough-relieving is stopped blooding. For cough due to lung-heat, tonsillitis, eye conjunctivitis, furuncle swelling toxin, Agkistrodon halys bites, and knife injury is hemorrhage. " Guizhou folk medicine " carries: " heat-clearing and toxic substances removing. Control tonsillitis, knife injury, innominate toxic swelling. "

Folium cinnamomi camphorae: acrid in the mouth, hardship, warm in nature; GUIXIN, spleen, lung meridian. Expelling wind and removing dampness, detoxify parasite killing. For rheumatic arthralgia, stomachache, burn due to hot liquid or fire, sore swollen toxin, chronic ulcer of the lower limb, scabies, skin pruritus, insect-bite. " Nanning City's medicine will " carries: " dispel the wind, reduce phlegm, parasite killing. Control cold rheumatic ostalgia, stomachache. External treatment traumatic injury flutters wound, dissipates poison, circulation of qi promoting, pain relieving. "

Cortex Moutan: bitter in the mouth, pungent, cold nature; GUIXIN, liver, kidney channel. Clearing away heat and cooling blood, blood circulation promoting and blood stasis dispelling. For maculae caused by violent heat pathogen, hematemesis and epistaxis, night fever abating at dawn, lossless hectic fever due to YIN-deficiency, amenorrhea dysmenorrhea, carbuncle sore tumefacting virus, fall and flutter the pain of injury.

Staphylococcus albus, bacillus subtilis, escherichia coli, Bacillus typhi etc. are had stronger antibacterial action by Cortex Moutan.

Bulbus Allii: acrid in the mouth, warm in nature; Return spleen, stomach, lung, large intestine channel. Warming middle-JIAO row is stagnant, removing toxic substances, parasite killing. For coldness and pain in the epigastrium, dysentery, have loose bowels, pulmonary tuberculosis, pertussis, flu, carbuncle furuncle and phyma poison, acute appendicitis, tinea skin ulcer, snake bite and insect sting, ancylostomiasis, enterobiasis, leukorrhagia pudendal pruritus, malaria, sore throat, edema. " not Lu " load: " dissipate carbuncle skin ulcer, exorcising, gas of killing virus. " modern medicine study confirmation, pyococcus, tubercule bacillus, dysentery bacterium, Bacillus typhi, Salmonella paratyphi, vibrio cholera etc. are in vitro all had inhibitory action by uncooked garlic.

Rhizoma Alismatis: sweet in the mouth, cold in nature; Return kidney, bladder warp. Diuresis, clearing away damp-heat. For dysuria, edema distension, oliguria of having loose bowels, dizziness due to fluid-retention, the puckery pain of pyretic stranguria, hyperlipidemia. " herbal classic " carries: " main anemofrigid-damp arthralgia, breast is difficult, and disappear water, supports the five internal organs, physical strength profiting, and fertilizer is strong. "

Folium Jujubae: sweet in the mouth, warm in nature. Heat-clearing and toxic substances removing. For fever of children, furuncle, miliaria alba, rotten foot, burn and scald. " book on Chinese herbal medicine asks former " carries: " wash infantile malnutrition, hemorrhoid, treatment, rotten foot, knot poison. Branch endures cream sterilization. "

Herba Oxalidis Corniculatae: sour in the mouth, cold in nature. Clearing away heat-damp and promoting diuresis, cool the blood dissipate blood stasis, subduing swelling and detoxicating. For dysentery, jaundice gonorrhea, leucorrhea with red and white discharge, measles is spitted blood, laryngopharynx swelling and pain, furuncle carbuncle, scabies haemorrhoids, proctoptosis, traumatic injury, burn. Tang Materia Medica carry: " the lame fistula of main malignant boil smash deposited it, kill all small worms. " " food antipyretic yearningly. " modern medicine study confirmation, staphylococcus aureus is had antibacterial action by Herba Oxalidis Corniculatae.

Spina Gleditsiae: acrid in the mouth, warm in nature; Return liver, lung meridian. For controlling swollen ulcer drug, scrofula, skin ulcer rash stubborn dermatitis, puerperal hypogalactia, placenta retention, leprosy. Modern medicine study confirms, staphylococcus aureus and other coccuses are had inhibitory action by Spina Gleditsiae decoct.

The present invention compared with prior art, has the advantages that

Selected by disinfectant solution of the present invention, medical material compatibility is suitable, meet Chinese medicine and modern medicine is theoretical, there is heat-clearing and toxic substances removing, bacteriostasis and sterilization, parasite killing antiinflammatory, soluble in water, stability high, and human body skin is non-stimulated, stability is high, safe without toxic side effect, can be widely applied to clinical laboratory's personnel's skin and the sterilization to indoor environment, improves the work efficiency of clinical laboratory personnel, ensure that the accuracy rate of assay, be a kind of antibacterial disinfectant solution of the ideal being worthy to be popularized.

Detailed description of the invention

Below in conjunction with specific embodiment, the present invention is further elaborated.

Embodiment 1

The raw material of disinfectant solution effective ingredient of the present invention forms and weight is:

Radix Seu Folium Ecdysantherae Roseae 84g Neoalsomitra integrifoliola (Cogn.) Hucth. 84g Herba Polygoni Avicularis 84g Folium catalpae ovatae 79g

Herba Clematidis Buchananianae 79g dish 79g Herba Limnophilae rugosae 74g Rhizoma Zingiberis 74g

Disinfectant 67g Folium cinnamomi camphorae 67g Cortex Moutan 67g Bulbus Allii 61g

Rhizoma Alismatis 61 Folium Jujubae 61g Herba Oxalidis Corniculatae 54g Spina Gleditsiae 44g.

The preparation method of disinfectant solution of the present invention, comprises the following steps that:

(1) Radix Seu Folium Ecdysantherae Roseae, Herba Clematidis Buchananianae, Herba Limnophilae rugosae, Rhizoma Zingiberis, disinfectant, Bulbus Allii and Spina Gleditsiae decontamination are cleaned, be placed in container, add the distilled water immersion of 8-10 times amount after 4-6 hour, boil 3-4 hour, extract; Again add 5-7 times amount distilled water, boil 2-3 hour, extract; Finally, add 2-4 times amount distilled water, heated and boiled 1-2 hour, extract; Merge three extracting solution, filter, obtain filtrate, standby;

Embodiment 2

Radix Seu Folium Ecdysantherae Roseae 76g Neoalsomitra integrifoliola (Cogn.) Hucth. 76g Herba Polygoni Avicularis 76g Folium catalpae ovatae 72g

Herba Clematidis Buchananianae 72g dish 72g Herba Limnophilae rugosae 65g Rhizoma Zingiberis 65g

Disinfectant 58g Folium cinnamomi camphorae 58g Cortex Moutan 58g Bulbus Allii 53g

Rhizoma Alismatis 53g Folium Jujubae 53g Herba Oxalidis Corniculatae 45g Spina Gleditsiae 38g.

The disinfectant solution preparation method processing step of the present embodiment is identical with embodiment 1.

Embodiment 3

Radix Seu Folium Ecdysantherae Roseae 69g Neoalsomitra integrifoliola (Cogn.) Hucth. 69g Herba Polygoni Avicularis 69g Folium catalpae ovatae 64g

Herba Clematidis Buchananianae 64g dish 64g Herba Limnophilae rugosae 59g Rhizoma Zingiberis 59g

Disinfectant 52g Folium cinnamomi camphorae 52g Cortex Moutan 52g Bulbus Allii 47g

Rhizoma Alismatis 47g Folium Jujubae 47g Herba Oxalidis Corniculatae 40g Spina Gleditsiae 34g.

Embodiment 4

Radix Seu Folium Ecdysantherae Roseae 62g Neoalsomitra integrifoliola (Cogn.) Hucth. 62g Herba Polygoni Avicularis 62g Folium catalpae ovatae 58g

Herba Clematidis Buchananianae 58g dish 58g Herba Limnophilae rugosae 53g Rhizoma Zingiberis 53g

Disinfectant 46g Folium cinnamomi camphorae 46g Cortex Moutan 46g Bulbus Allii 41g

Rhizoma Alismatis 46g Folium Jujubae 46g Herba Oxalidis Corniculatae 46g Spina Gleditsiae 29g.

Experimental section

Below the selection present invention is tested accordingly, prove effect of the present invention further.

One, soluble test

1, take clinical laboratory of the present invention and put in container with antibacterial disinfectant solution 10ml, be subsequently adding distilled water, after stirring, filter.

2, after having filtered, filter paper, the obvious residue of thing on filter paper are observed.

3, conclusion: clinical laboratory of the present invention is highly soluble in water with antibacterial disinfectant solution, uses rear noresidue impurity to occur.

Two, toxicity test

1, acute oral test: choose the healthy Kunming kind one-level mice 20 of quality 20-22g, male and female half and half, by clinical laboratory of the present invention antibacterial disinfectant solution per os gastric infusion, administration observation 15 days, record the various performance of mice and death condition every day, calculate LD. value.

2, cumulative toxicity test: choose the healthy Kunming kind one-level rat 20 of quality 180-200g, male and female

Half and half, by clinical laboratory of the present invention antibacterial disinfectant solution per os gastric infusion, the DL. value that dosage draws according to acute oral test, the 1-6 days, it is administered 0.1LD. every time, within later every four hours, is incremented by 1.5 multiple doses, it is administered 30 days, and every four hours weigh once, calculate cumulative coefficient.

3, there is not death in result: no abnormality seen reaction after acute oral test mice perfusion, and after it is carried out dissection accordingly, tissue or internal organs have no and substantially have obvious abnormal change.

After cumulative toxicity test is administered 30 days, male and female mice all occurs without death condition, mice cumulative coefficient 5, the weak cumulative toxicity in position.

Three, Ocular irritation test

1, healthy white rabbit 6 is selected, weight 2.5-3.0kg, clinical laboratory of the present invention is instilled in rabbit conjunctiva of left eye capsule with antibacterial disinfectant solution stock solution 0.1ml, with normal saline flushing after passive Guan Bi 4s, right eye instills normal saline as comparison.

2, rabbit 12 hours, 24 hours, 36 hours and 48 hours interior eyes localized variation are observed.

3, result: rabbit contamination group cornea and iris are normal, and conjunctiva has no edema, and having slight hyperemia, conjunctiva stimulation index is 1, by acute ocular stimulus intensity analytic intensity grade scale, belongs to nonirritant.

Four, irritation test

1, healthy white rabbit 6 is selected, weight 2.5-3.50kg, lose hair or feathers respectively 3.0cm �� 3.0cm by family's rabbit back spinal column both sides, does not damage epidermis, after 24 hours, by clinical laboratory of the present invention for 0.2ml with antibacterial disinfectant solution uniform application in the left side of baring skin, right side is not smeared as comparison, after 5 hours, skin wiping is clean, observe dermoreaction, carry out stimulating scoring.

2, result: rabbit baring skin reacts there are no erythema and edema, and skin wound repair integrated value is 0, control sides skin no abnormality seen, by skin wound repair standards of grading, reaction integral mean value is 0, and disinfectant solution of the present invention is to no skin irritation.

Five, stability test

Take the antibacterial disinfectant solution 50ml of clinical laboratory of the present invention, after placing 90 days under 37 DEG C of conditions, for staphylococcus aureus is sterilized, before its killing effect and storage, compare no significant difference.

Six, bacteriostatic test

Below clinical laboratory of the present invention is carried out bacteriostatic experiment with antibacterial disinfectant solution:

1, experimental strain:

Select the strain that laboratory is representative, staphylococcus aureus and bacillus pyocyaneus.

2, bacterium culture medium:

Nutrient agar and nutrient broth medium.

3, experiment classification:

Bacteriostatic experiment divides experimental group and matched group, and experimental group selects the antibacterial disinfectant solution of clinical laboratory of the present invention, and matched group selects lysol.

4, specific experiment step:

(1) preparation of bacterium solution: above two strain is inoculated in respectively on two agar culture mediums, cultivating 24 hours at 37 DEG C, from above-mentioned agar culture medium, picking colonies typical is inoculated in two fresh nutrient broth mediums respectively respectively, under synthermal, cultivate 24 hours, standby;

(2) take the clinical laboratory of the present invention each 30ml of antibacterial disinfectant solution and lysol, be respectively placed in cut-back tank, it is diluted respectively 25 times, above two diluent solution is split in two containers respectively, is placed under aseptic condition, standby;

(3) with aseptic nutrient broth, the experimental strain that above-mentioned steps (1) is supported 24 hours through nutrition broth cultivation being diluted 50 times respectively, every kind of strain is sub-packed in two culture dishs, respectively labelling matched group and experimental group;

(4) the Liang Fen clinical laboratory of the present invention dilute solution of antibacterial disinfectant solution of above-mentioned steps (2) gained being respectively dropped into experimental group culture dish, two parts of lysol dilute solutions are respectively dropped into matched group culture dish.

5, experimental observation is measured:

Above-mentioned experiment is observed, observes two groups of strains and measure strain revolution mark concrete outcome.

6, after observation is measured, above-mentioned culture dish is placed under aseptic condition and stands two days, treat that liquid is complete

After full volatilization, follow the tracks of it has been observed that matched group bacterium colony continues breeding, and experimental group bacterium colony is unchanged.

7, experimental result:

The experimental group clinical laboratory of the present invention sterilizing ability of the antibacterial disinfectant solution sterilizing ability much larger than matched group disinfectant solution, and all kinds of strain can be suppressed to continue breeding.

Claims

1. the antibacterial disinfectant solution of clinical laboratory, it is characterised in that: make described disinfectant solution effective ingredient raw material composition and parts by weight be:

2. the antibacterial disinfectant solution of a kind of clinical laboratory according to claim 1, it is characterised in that: make the raw material of described disinfectant solution effective ingredient and form and parts by weight are:

3. the antibacterial disinfectant solution of a kind of clinical laboratory according to claim 1, it is characterised in that: make the raw material of described disinfectant solution effective ingredient and form and parts by weight are:

4. the antibacterial disinfectant solution of a kind of clinical laboratory according to claim 1, it is characterised in that: make the raw material of described disinfectant solution effective ingredient and form and parts by weight are:

5. the preparation method of antibacterial disinfectant solution of the clinical laboratory as described in any one of claim 1-4, it is characterised in that: comprise the following steps that: