CN105641552A - Sterilization disinfectant for clinical laboratory and preparation method - Google Patents

Sterilization disinfectant for clinical laboratory and preparation method Download PDF

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CN105641552A
CN105641552A CN201610106549.8A CN201610106549A CN105641552A CN 105641552 A CN105641552 A CN 105641552A CN 201610106549 A CN201610106549 A CN 201610106549A CN 105641552 A CN105641552 A CN 105641552A
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herba
radix
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clinical laboratory
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孙永丽
梁英
辛丽梅
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    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
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Abstract

The invention discloses a sterilization disinfectant for the clinical laboratory and a preparation method and belongs to the field of traditional Chinese medicines. The disinfectant comprises effective ingredients of raw materials as follows: tribulus terrestris, bletilla, Eriolaena spectabilis, common dysosmatis rhizomes and roots, paper mulberry stems, barbed skullcap herbs, Astragalus sinicus, edible amaranth, betel nuts, Ardisia maculosa Mez, roots of Job's tears, corydalis bungeana, figs, cactus, virgate wormwood herbs and cherry leaves. According to the disinfectant, the medicinal materials are matched properly and are compatible, the sterilization disinfectant has the advantages of high stability, no corrosion, no toxicity, easiness in dissolving in water and low cost, has high sterilization capability, takes effect rapidly, has no irritation to skin of human bodies, can be widely used for disinfecting skin of workers in the clinical laboratory and is worthy of wide popularization and application, the working efficiency of the workers in the clinical laboratory can be improved, and the accuracy of the inspection result is guaranteed.

Description

A kind of clinical laboratory's disinfection solution and preparation method
Art
The present invention relates to the field of Chinese medicines, particularly relate to a kind of clinical laboratory's disinfection solution and preparation method.
Background technology
The important section office that the various patient specimen of Shi Quan institute of clinical laboratory focuses on, clinical laboratory staff is because of the particularity of its occupation, have to every day be exposed among various risk factor, such as all kinds of infectious diseases, various disinfectant, various antibacterials etc., therefore injury it is highly susceptible to, and the sterilizing medicine such as iodine tincture that inspection institute uses, ethanol, povidone iodine etc. are made up of chemicals mostly, human body wound and skin are had serious zest by the chemicals of this kind, it is unfavorable for the healing of wound wound, what have even can cause serious anaphylaxis, if sterilizing is not thorough, it is easy to fester a wound, can have a strong impact on, sb.'s illness took a favorable turn and normal life to incur loss through delay patient.
For this, when medical institutions carry out medical test, the bacterial flora system of checking system should minimize, the trier that medical personnel itself and inspection are required is just carried out omnibearing sterilizing thoroughly by this, eliminate a series of antibacterial and pathogen such as staphylococcus aureus, escherichia coli, Pseudomonas aeruginosa, proteus vulgaris etc., provide good environment for checking system.
Therefore, find that a kind of disinfection effect is good and the disinfectant solution of nonirritant and side effect, clinical laboratory's every inspection work is had critically important meaning.
Summary of the invention
The invention provides a kind of clinical laboratory's disinfection solution and preparation method; Selected by disinfectant solution of the present invention, medical material compatibility is suitable, all medicine phases and have that stability is strong, non-corrosiveness, without any toxicity, advantage soluble in water and with low cost, and sterilizing ability is strong, speed of action is fast, human body skin is non-stimulated, can be widely applied to the sterilization of clinical laboratory's personnel's skin, the work efficiency of clinical laboratory personnel can be improved, ensureing the accuracy rate of assay, should be widely promoted application.
For achieving the above object, technical scheme is implemented as follows:
A kind of clinical laboratory disinfection solution, raw material composition and the parts by weight of making described disinfectant solution effective ingredient are:
54��78 parts of Pseudobulbus Bletillae (Rhizoma Bletillae) of Fructus Tribuli, 52��76 portions of Radix et Rhizoma Dysosmatiss of 53��77 parts of Bark of Lindenleaf Torricellia 52��76 parts
51��75 portions of Herba Scutellariae Barbataes of Ramulus Broussonetiae, 50��74 parts of Amaranthus mangostanus L.s of 51��75 parts of Herba Astragali Melilotoidis (Herba Astragali Sinici)s 49��73 parts
49��73 parts of Herba Ardisiae Maculosaes of Semen Arecae, 47��71 portions of Herba corydalis eduliss of 48��72 parts of Radix Coiciss 47��71 parts
47��71 parts of Radix et Caulis Opuntiae Dilleniis of Fructus Fici, 43��67 parts of Folium Pruni pseudocerasis of 46��69 portions of Herba Artemisiae Scopariaes 43��67 parts.
Make described disinfectant solution effective ingredient raw material composition and parts by weight be:
58��74 parts of Pseudobulbus Bletillae (Rhizoma Bletillae) of Fructus Tribuli, 56��72 portions of Radix et Rhizoma Dysosmatiss of 57��73 parts of Bark of Lindenleaf Torricellia 56��72 parts
55��71 portions of Herba Scutellariae Barbataes of Ramulus Broussonetiae, 54��70 parts of Amaranthus mangostanus L.s of 55��71 parts of Herba Astragali Melilotoidis (Herba Astragali Sinici)s 53��69 parts
53��69 parts of Herba Ardisiae Maculosaes of Semen Arecae, 51��67 portions of Herba corydalis eduliss of 52��68 parts of Radix Coiciss 51��67 parts
51��67 parts of Radix et Caulis Opuntiae Dilleniis of Fructus Fici, 47��63 parts of Folium Pruni pseudocerasis of 49��65 portions of Herba Artemisiae Scopariaes 47��63 parts.
Make described disinfectant solution effective ingredient raw material composition and parts by weight be:
62��70 parts of Pseudobulbus Bletillae (Rhizoma Bletillae) of Fructus Tribuli, 60��68 portions of Radix et Rhizoma Dysosmatiss of 61��69 parts of Bark of Lindenleaf Torricellia 60��68 parts
59��67 portions of Herba Scutellariae Barbataes of Ramulus Broussonetiae, 58��66 parts of Amaranthus mangostanus L.s of 59��67 parts of Herba Astragali Melilotoidis (Herba Astragali Sinici)s 57��65 parts
57��65 parts of Herba Ardisiae Maculosaes of Semen Arecae, 55��63 portions of Herba corydalis eduliss of 56��64 parts of Radix Coiciss 55��63 parts
55��63 parts of Radix et Caulis Opuntiae Dilleniis of Fructus Fici, 51��59 parts of Folium Pruni pseudocerasis of 53��61 portions of Herba Artemisiae Scopariaes 51��59 parts.
Make described disinfectant solution effective ingredient raw material composition and parts by weight be:
66 parts of Pseudobulbus Bletillae (Rhizoma Bletillae) of Fructus Tribuli, 64 portions of Radix et Rhizoma Dysosmatiss of 65 parts of Bark of Lindenleaf Torricellia 64 parts
63 portions of Herba Scutellariae Barbataes of Ramulus Broussonetiae, 62 parts of Amaranthus mangostanus L.s of 63 parts of Herba Astragali Melilotoidis (Herba Astragali Sinici)s 61 parts
61 parts of Herba Ardisiae Maculosaes of Semen Arecae, 59 portions of Herba corydalis eduliss of 60 parts of Radix Coiciss 59 parts
59 parts of Radix et Caulis Opuntiae Dilleniis of Fructus Fici, 55 parts of Folium Pruni pseudocerasis of 57 portions of Herba Artemisiae Scopariaes 55 parts.
The preparation method of a kind of clinical laboratory disinfection solution, comprises the following steps that:
(1) Fructus Tribuli, Pseudobulbus Bletillae (Rhizoma Bletillae), Bark of Lindenleaf Torricellia, Herba Scutellariae Barbatae, Radix Coicis, Herba corydalis edulis, Fructus Fici and Herba Artemisiae Scopariae are cleaned, put into container, add the distilled water of 8��10 times amount, after soaking 5��6 hours, boil 3��4 hours, extract; Again add 6��8 times amount distilled water, boil 2��3 hours, extract; Finally, add 4��6 times amount distilled water, heated and boiled 1��2 hour, extract; Merge three extracting solution, filter, obtain filtrate, standby;
(2) Radix et Rhizoma Dysosmatis, Herba Ardisiae Maculosae and Radix et Caulis Opuntiae Dillenii are cleaned, immerse 5 times amount 80% alcohol solution dipping 1��2 hour, heating extraction 3 hours, reclaim ethanol, filter, obtain filtrate, concentration is that at 65 DEG C, relative density is the thick paste of 1.12��1.16, standby;
(3) Ramulus Broussonetiae, Amaranthus mangostanus L. and Folium Pruni pseudocerasi are cleaned, chopping, put into container, twist juice, filter impurity, stay juice standby;
(4) Herba Astragali Melilotoidis (Herba Astragali Sinici) and Semen Arecae are put into container, carry out pulverization process, grind into powder, obtain the fine powder of 200 orders, standby;
(5) after the filtrate obtained by above-mentioned steps (1)��(4), thick paste, juice and fine powder being mixed, add the distilled water of 10��12 times amount, after stirring and dissolving is uniform, heated and boiled 0.5 hour, to less than 60 DEG C, stand 3 hours, filter, obtain filtrate, filtrate fill is sealed up for safekeeping, 121 DEG C of pressure sterilizings 20 minutes, packaging, it is described clinical laboratory's disinfection solution.
The property of medicine of Chinese medicine used by the present invention is as follows:
Fructus Tribuli: toil, temperature; Enter liver, lung meridian. Dissipate wind improving eyesight, therapeutic method to keep the adverse QI flowing downwards promoting the circulation of blood. Main headache, dizziness, distending pain in the chest and hypochondrium, distending pain of the breast, breast closes obstructed, amenorrhea, cataract, rubella pruritus, vitiligo, skin ulcer cellulitis, scrofula. " not Lu " load: " main health pruritus due to wind pathogen, headache, cough with dyspnea impairing the lung, consumptive lung disease, only tired, the therapeutic method to keep the adverse QI flowing downwards; Children's's head sore, carbuncle the moon Huang, can rub powder. "
Pseudobulbus Bletillae (Rhizoma Bletillae): acrid in the mouth, hardship, sweet, puckery, cold nature; Return lung, liver, stomach warp. Tonifying the lung, hemostasis, detumescence, granulation promoting, sore. Controlling lung and hinder hemoptysis, epistaxis, incised wound is hemorrhage, swollen ulcer drug, canker sores, and soup fire is burnt, rhagadia manus et pedis." Bencao Tujing " carry: " control incised wound not to be recovered, in carbuncle side multiplex it. " modern medicine study confirmation, Pseudobulbus Bletillae (Rhizoma Bletillae) can strengthen platelet factor III activity, and notable shortening clotting time and thrombinogen form the time, it is suppressed that plasmin enzymatic activity, and local hemorrhage is had anastalsis, and its water decoction has antibacterial action.
Bark of Lindenleaf Torricellia: bitter in the mouth, puckery, cool in nature. Astringing to arrest bleeding, reuniting the fractured tendons and bones. Cure mainly traumatic hemorrhage, burn and scald, chronic gastritis, gastric ulcer. Also can control weapons wound, the disease such as fracture.
Radix et Rhizoma Dysosmatis: bitter in the mouth, cool in nature. Heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling. Cure mainly: venom, traumatic injury; Worm venom, ulcerative carbuncle furuncle and phyma, lymphadenitis, parotitis, breast carcinoma are controlled in external. Guangzhou army " conventional Chinese herbal medicine handbook " load: heat-clearing and toxic substances removing, dampness pathogenic fire purging. Control lymphadenitis, parotitis, ulcerative carbuncle. "
Ramulus Broussonetiae: dispel the wind, improving eyesight, diuresis. Cure mainly rubella, conjunctival congestion and swelling pain, dysuria. " detailed outline " carries: " smashes extract and drinks half liter, control urinary obstruction. "
Herba Scutellariae Barbatae: acrid in the mouth, hardship, cold in nature; Return lung, liver, kidney channel. Heat-clearing and toxic substances removing, blood stasis dispelling diuresis. For furuncle swelling toxin, laryngopharynx swelling and pain, venom, fall and flutter the pain of injury, edema, jaundice. Modern medicine study confirms, staphylococcus aureus, shigella flexneri, Bacillus typhi, bacillus pyocyaneus, escherichia coli are had inhibitory action by Herba Scutellariae Barbatae 50% decoct.
Herba Astragali Melilotoidis (Herba Astragali Sinici): acrid in the mouth, property is put down, nontoxic. Removing blood stasis hemostasia and promoting granulation, profit supports the five internal organs, bright knowledge, hot blast of dispelling, and makes us spry and light, and food is not minded for a long time, mends people. Only hot malaria, flat stomach of invigorating blood circulation. Modern medicine study proves, Herba Astragali Melilotoidis (Herba Astragali Sinici) have invigorate blood circulation, hearing-improing and eyesight improving, the function such as make light of one's life by commiting suicide, make people's skin moisten, energetic, defying age.
Amaranthus mangostanus L.: sweet in the mouth, cold nature; Enter large intestine, small intestine meridian. Heat-clearing and toxic substances removing, smoothening secretion. Cure mainly dysentery, constipation and anuresis, snakeworm sting, sore. " the southern regions of the Yunnan Province book on Chinese herbal medicine " load: " control sphincter disturbance, change worm cold removing heat, energy promoting blood circulation, removing blood stasis blood. " modern medicine study confirmation, staphylococcus aureus, Staphylococcus albus, Streptococcus viridans, colibacillus, Pseudomonas aeruginosa and klebsiella bacillus are had stronger antibacterial action by the nalka hydro carbons in Amaranthus mangostanus L. extract, nalka alcohols, sterols.
Semen Arecae: bitter in the mouth, pungent, warm in nature; Return stomach, large intestine channel. Killing insect and eliminating accumulation, sending down the abnormal ascending QI, Hang Shui, preventing the attack (or recurrence) of malaria. For cestode, ascarid, fasciolopsiasis buski, abdominal pain due to worm stagnation, stagnant dysentery, tenesmus, edema beriberi, malaria. Modern medicine study confirms, the dermatophytess such as trichophyton are in vitro had inhibitory action in various degree, decoct and water logging agent some strain of infected by influenza A type to have certain inhibitory action by Semen Arecae infusion.
Herba Ardisiae Maculosae: acrid in the mouth, hardship, slightly warm in nature; Return liver, kidney, lung meridian. Promoting blood circulation and stopping pain, clearing throat. Main traumatic injury, fracture, rheumatic arthralgia, diphtheria, laryngopharynx swelling and pain, gastric ulcer.
Radix Coicis: bitter in the mouth, sweet, cold in nature; Enter spleen, bladder warp. Clearing heat and freeing strangury, plants wet parasite killing. Main pyretic stranguria, stranguria with blood, stranguria caused by urinary stone, jaundice, edema, leukorrhagia, beriberi, rheumatic arthralgia, ascariasis. " the southern regions of the Yunnan Province book on Chinese herbal medicine " load: " clear diuresis. Controlling pyretic stranguria pain, hematuria, hemostasis is drenched, beautiful stem pain, consumer edema. "
Herba corydalis edulis: bitter in the mouth, puckery, cool in nature; Return lung, kidney, spleen channel. Heat-clearing and toxic substances removing, killing parasites for relieving itching. Have a headache for heatstroke, stomachache, dysurea, hemoptysis of pulmonary tuberculosis;Suppurative otitis media, proctoptosis, sore swollen toxin, venom are controlled in external. Pharmacological evaluation shows, staphylococcus aureus has in Herba corydalis edulis 25% decocting liquid test tube significant inhibitory action,
Escherichia coli, bacillus pyocyaneus are taken second place.
Fructus Fici: sweet in the mouth, cool in nature; Return lung, stomach, large intestine channel. Invigorating the stomach and promoting bowel movement, subduing swelling and detoxicating. Control enteritis, dysentery, constipation, hemorrhoid, laryngalgia, ulcerative carbuncle scabies, throat, anthelmintic of whetting the appetite. For inappetence, abdominal distention, constipation due to hemorrhoids, dyspepsia, hemorrhoid, proctoptosis, diarrhoea, hypogalactia, laryngopharynx swelling and pain, hematodiarrhoea, the disease such as cough ant phlegm. " Yunnan Chinese herbal medicine " carries: " stomach invigorating antidiarrheal, eliminates the phlegm and regulate the flow of vital energy. Control inappetence, dyspepsia, enteritis, dysentery, throat pain, cough with copious phlegm, uncomfortable in chest. "
Radix et Caulis Opuntiae Dillenii: bitter in the mouth, cool in nature. Heat-clearing and toxic substances removing, dissipating blood stasis for subsidence of swelling, benefiting stomach and stopping pain, antitussive. For taste-blindness rate, acute dysentery, cough; Mumps, mastitis, carbuncle furuncle and phyma poison, venom, burn and scald are controlled in external. " east, Fujian book on Chinese herbal medicine " load: " energy blood stasis removing, intestinal detoxication, stomach invigorating, pain relieving, nourishing, relaxing muscles and tendons and activating QI and blood in the collateral, hemostasis of curing the wound. Control hemorrhoidal hamorrhage anal fistula hematochezia, lung abscess, stomachache, traumatic injury. "
Herba Artemisiae Scopariae: bitter in the mouth, pungent, warm in nature; Return spleen, stomach, liver, gallbladder meridian. Heat clearing away, dampness removing, jaundice eliminating. Cure mainly jaundice, dysuria, eczema pruritus, infectious jaundice type hepatitis etc. Pharmaceutical research confirms, staphylococcus aureus, diphtheria corynebacterium, anthrax bacillus, Bacillus typhi, paratyphoid bacillus A, bacillus pyocyaneus, escherichia coli, bacterium flexneri, dysentery bacterium, meningococcus, bacillus subtilis etc. are had inhibitory action in various degree by Herba Artemisiae Scopariae decoct.
Folium Pruni pseudocerasi: sweet-bitter flavor, warm in nature, nontoxic; Return liver, spleen, lung meridian. Warming middle-JIAO and strengthening the spleen, cough-relieving is stopped blooding, and detoxify parasite killing. Main cold syndrome of the stomach food stagnation, diarrhoea, cough, to spit blood, skin infection swells carbuncle, snake bite and insect sting, trichomonas vaginitis. " book on Chinese herbal medicine is new again " carries: " nourishing the liver helps fire, spleen benefiting and stimulating the appetite, and long-pending except gastral cavilty trembles with fear, and helps digestion brokenly stagnant. "
The present invention compared with prior art, has the advantages that
Selected by disinfectant solution of the present invention, medical material compatibility is suitable, all medicine phases and have that stability is strong, non-corrosiveness, without any toxicity, advantage soluble in water and with low cost, and sterilizing ability is strong, speed of action is fast, human body skin is non-stimulated, can be widely applied to the sterilization of clinical laboratory's personnel's skin, the work efficiency of clinical laboratory personnel can be improved, ensureing the accuracy rate of assay, should be widely promoted application.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further elaborated.
Embodiment 1
The raw material of disinfectant solution effective ingredient of the present invention forms and weight is:
Fructus Tribuli 74g Pseudobulbus Bletillae (Rhizoma Bletillae) 73g Bark of Lindenleaf Torricellia 72g Radix et Rhizoma Dysosmatis 72g
Ramulus Broussonetiae 71g Herba Scutellariae Barbatae 71g Herba Astragali Melilotoidis (Herba Astragali Sinici) 70g Amaranthus mangostanus L. 69g
Semen Arecae 69g Herba Ardisiae Maculosae 68g Radix Coicis 67g Herba corydalis edulis 67g
Fructus Fici 67g Radix et Caulis Opuntiae Dillenii 65g Herba Artemisiae Scopariae 63g Folium Pruni pseudocerasi 63g.
The preparation method of a kind of clinical laboratory disinfection solution, comprises the following steps that:
(1) Fructus Tribuli, Pseudobulbus Bletillae (Rhizoma Bletillae), Bark of Lindenleaf Torricellia, Herba Scutellariae Barbatae, Radix Coicis, Herba corydalis edulis, Fructus Fici and Herba Artemisiae Scopariae are cleaned, put into container, add the distilled water of 8��10 times amount, after soaking 5��6 hours, boil 3��4 hours, extract; Again add 6��8 times amount distilled water, boil 2��3 hours, extract; Finally, add 4��6 times amount distilled water, heated and boiled 1��2 hour, extract;Merge three extracting solution, filter, obtain filtrate, standby;
(2) Radix et Rhizoma Dysosmatis, Herba Ardisiae Maculosae and Radix et Caulis Opuntiae Dillenii are cleaned, immerse 5 times amount 80% alcohol solution dipping 1��2 hour, heating extraction 3 hours, reclaim ethanol, filter, obtain filtrate, concentration is that at 65 DEG C, relative density is the thick paste of 1.12��1.16, standby;
(3) Ramulus Broussonetiae, Amaranthus mangostanus L. and Folium Pruni pseudocerasi are cleaned, chopping, put into container, twist juice, filter impurity, stay juice standby;
(4) Herba Astragali Melilotoidis (Herba Astragali Sinici) and Semen Arecae are put into container, carry out pulverization process, grind into powder, obtain the fine powder of 200 orders, standby;
(5) after the filtrate obtained by above-mentioned steps (1)��(4), thick paste, juice and fine powder being mixed, add the distilled water of 10��12 times amount, after stirring and dissolving is uniform, heated and boiled 0.5 hour, to less than 60 DEG C, stand 3 hours, filter, obtain filtrate, filtrate fill is sealed up for safekeeping, 121 DEG C of pressure sterilizings 20 minutes, packaging, it is described clinical laboratory's disinfection solution.
Embodiment 2
The raw material of disinfectant solution effective ingredient of the present invention forms and weight is:
Fructus Tribuli 70g Pseudobulbus Bletillae (Rhizoma Bletillae) 69g Bark of Lindenleaf Torricellia 68g Radix et Rhizoma Dysosmatis 68g
Ramulus Broussonetiae 67g Herba Scutellariae Barbatae 67g Herba Astragali Melilotoidis (Herba Astragali Sinici) 66g Amaranthus mangostanus L. 65g
Semen Arecae 65g Herba Ardisiae Maculosae 64g Radix Coicis 63g Herba corydalis edulis 63g
Fructus Fici 63g Radix et Caulis Opuntiae Dillenii 61g Herba Artemisiae Scopariae 59g Folium Pruni pseudocerasi 59g.
The disinfectant solution preparation method processing step of the present embodiment and usage and dosage and embodiment 1 are identical.
Embodiment 3
The raw material of disinfectant solution effective ingredient of the present invention forms and weight is:
Fructus Tribuli 66g Pseudobulbus Bletillae (Rhizoma Bletillae) 65g Bark of Lindenleaf Torricellia 64g Radix et Rhizoma Dysosmatis 64g
Ramulus Broussonetiae 63g Herba Scutellariae Barbatae 63g Herba Astragali Melilotoidis (Herba Astragali Sinici) 62g Amaranthus mangostanus L. 61g
Semen Arecae 61g Herba Ardisiae Maculosae 60g Radix Coicis 59g Herba corydalis edulis 59g
Fructus Fici 59g Radix et Caulis Opuntiae Dillenii 57g Herba Artemisiae Scopariae 55g Folium Pruni pseudocerasi 55g.
The disinfectant solution preparation method processing step of the present embodiment and usage and dosage and embodiment 1 are identical.
Embodiment 4
The raw material of disinfectant solution effective ingredient of the present invention forms and weight is:
Fructus Tribuli 62g Pseudobulbus Bletillae (Rhizoma Bletillae) 61g Bark of Lindenleaf Torricellia 60g Radix et Rhizoma Dysosmatis 60g
Ramulus Broussonetiae 59g Herba Scutellariae Barbatae 59g Herba Astragali Melilotoidis (Herba Astragali Sinici) 58g Amaranthus mangostanus L. 57g
Semen Arecae 57g Herba Ardisiae Maculosae 56g Radix Coicis 55g Herba corydalis edulis 55g
Fructus Fici 55g Radix et Caulis Opuntiae Dillenii 53g Herba Artemisiae Scopariae 51g Folium Pruni pseudocerasi 51g.
The disinfectant solution preparation method processing step of the present embodiment and usage and dosage and embodiment 1 are identical.
Embodiment 5
The raw material of disinfectant solution effective ingredient of the present invention forms and weight is:
Fructus Tribuli 58g Pseudobulbus Bletillae (Rhizoma Bletillae) 57g Bark of Lindenleaf Torricellia 56g Radix et Rhizoma Dysosmatis 56g
Ramulus Broussonetiae 55g Herba Scutellariae Barbatae 55g Herba Astragali Melilotoidis (Herba Astragali Sinici) 54g Amaranthus mangostanus L. 53g
Semen Arecae 53g Herba Ardisiae Maculosae 52g Radix Coicis 51g Herba corydalis edulis 51g
Fructus Fici 51g Radix et Caulis Opuntiae Dillenii 49g Herba Artemisiae Scopariae 47g Folium Pruni pseudocerasi 47g.
The disinfectant solution preparation method processing step of the present embodiment and usage and dosage and embodiment 1 are identical.
Bacteriostatic test:
Below disinfectant solution of the present invention is carried out bacteriostatic experiment:
1, experimental strain:
Select the strain that laboratory is representative, staphylococcus aureus, escherichia coli and bacillus pyocyaneus.
2, bacterium culture medium:
Nutrient agar and nutrient broth medium.
3, experiment classification:
Bacteriostatic experiment divides experimental group and matched group, and experimental group selects disinfectant solution of the present invention, and matched group selects hypochlorous acid disinfectant solution.
4, specific experiment step:
(1) preparation of bacterium solution: above-mentioned three kinds of strains are inoculated on three agar culture mediums respectively, cultivating 24 hours at 37 DEG C, from above-mentioned agar culture medium, picking colonies typical is inoculated in three fresh nutrient broth mediums respectively respectively, under synthermal, cultivate 18 hours, standby;
(2) take disinfectant solution of the present invention and each 50ml of hypochlorous acid disinfectant solution, be respectively placed in cut-back tank, it is diluted respectively 30 times, above two diluent solution is split in three containers respectively, is placed under aseptic condition, standby;
(3) with aseptic nutrient broth, the experimental strain that above-mentioned steps (1) is supported 22 hours through nutrition broth cultivation being diluted 50 times respectively, every kind of strain is sub-packed in two culture dishs, respectively labelling matched group and experimental group;
(4) dilute solution of two parts of disinfectant solution of the present invention of above-mentioned steps (2) gained being respectively dropped into experimental group culture dish, three parts of hypochlorous acid disinfectant solution dilute solutions are respectively dropped into matched group culture dish.
5, experimental observation is measured:
Above-mentioned experiment is observed, observes two groups of strains and measure strain revolution mark concrete outcome.
6, after observation is measured, above-mentioned culture dish is placed under aseptic condition and stands two days, after medicament volatilizees completely, follow the tracks of it has been observed that matched group bacterium colony continues breeding, and experimental group bacterium colony is unchanged.
7, experimental result:
The sterilizing ability of experimental group disinfectant solution of the present invention is much larger than the sterilizing ability of matched group disinfectant solution, and all kinds of strain can be suppressed to continue breeding.

Claims (5)

1. clinical laboratory's disinfection solution, it is characterised in that: make described disinfectant solution effective ingredient raw material composition and parts by weight be:
54��78 parts of Pseudobulbus Bletillae (Rhizoma Bletillae) of Fructus Tribuli, 52��76 portions of Radix et Rhizoma Dysosmatiss of 53��77 parts of Bark of Lindenleaf Torricellia 52��76 parts
51��75 portions of Herba Scutellariae Barbataes of Ramulus Broussonetiae, 50��74 parts of Amaranthus mangostanus L.s of 51��75 parts of Herba Astragali Melilotoidis (Herba Astragali Sinici)s 49��73 parts
49��73 parts of Herba Ardisiae Maculosaes of Semen Arecae, 47��71 portions of Herba corydalis eduliss of 48��72 parts of Radix Coiciss 47��71 parts
47��71 parts of Radix et Caulis Opuntiae Dilleniis of Fructus Fici, 43��67 parts of Folium Pruni pseudocerasis of 46��69 portions of Herba Artemisiae Scopariaes 43��67 parts.
2. a kind of clinical laboratory according to claim 1 disinfection solution, it is characterised in that: raw material composition and the parts by weight of making described disinfectant solution effective ingredient are:
58��74 parts of Pseudobulbus Bletillae (Rhizoma Bletillae) of Fructus Tribuli, 56��72 portions of Radix et Rhizoma Dysosmatiss of 57��73 parts of Bark of Lindenleaf Torricellia 56��72 parts
55��71 portions of Herba Scutellariae Barbataes of Ramulus Broussonetiae, 54��70 parts of Amaranthus mangostanus L.s of 55��71 parts of Herba Astragali Melilotoidis (Herba Astragali Sinici)s 53��69 parts
53��69 parts of Herba Ardisiae Maculosaes of Semen Arecae, 51��67 portions of Herba corydalis eduliss of 52��68 parts of Radix Coiciss 51��67 parts
51��67 parts of Radix et Caulis Opuntiae Dilleniis of Fructus Fici, 47��63 parts of Folium Pruni pseudocerasis of 49��65 portions of Herba Artemisiae Scopariaes 47��63 parts.
3. a kind of clinical laboratory according to claim 1 disinfection solution, it is characterised in that: raw material composition and the parts by weight of making described disinfectant solution effective ingredient are:
62��70 parts of Pseudobulbus Bletillae (Rhizoma Bletillae) of Fructus Tribuli, 60��68 portions of Radix et Rhizoma Dysosmatiss of 61��69 parts of Bark of Lindenleaf Torricellia 60��68 parts
59��67 portions of Herba Scutellariae Barbataes of Ramulus Broussonetiae, 58��66 parts of Amaranthus mangostanus L.s of 59��67 parts of Herba Astragali Melilotoidis (Herba Astragali Sinici)s 57��65 parts
57��65 parts of Herba Ardisiae Maculosaes of Semen Arecae, 55��63 portions of Herba corydalis eduliss of 56��64 parts of Radix Coiciss 55��63 parts
55��63 parts of Radix et Caulis Opuntiae Dilleniis of Fructus Fici, 51��59 parts of Folium Pruni pseudocerasis of 53��61 portions of Herba Artemisiae Scopariaes 51��59 parts.
4. a kind of clinical laboratory according to claim 1 disinfection solution, it is characterised in that: raw material composition and the parts by weight of making described disinfectant solution effective ingredient are:
66 parts of Pseudobulbus Bletillae (Rhizoma Bletillae) of Fructus Tribuli, 64 portions of Radix et Rhizoma Dysosmatiss of 65 parts of Bark of Lindenleaf Torricellia 64 parts
63 portions of Herba Scutellariae Barbataes of Ramulus Broussonetiae, 62 parts of Amaranthus mangostanus L.s of 63 parts of Herba Astragali Melilotoidis (Herba Astragali Sinici)s 61 parts
61 parts of Herba Ardisiae Maculosaes of Semen Arecae, 59 portions of Herba corydalis eduliss of 60 parts of Radix Coiciss 59 parts
59 parts of Radix et Caulis Opuntiae Dilleniis of Fructus Fici, 55 parts of Folium Pruni pseudocerasis of 57 portions of Herba Artemisiae Scopariaes 55 parts.
5. the preparation method of clinical laboratory's disinfection solution as described in any one of Claims 1 to 4, it is characterised in that: comprise the following steps that:
(1) Fructus Tribuli, Pseudobulbus Bletillae (Rhizoma Bletillae), Bark of Lindenleaf Torricellia, Herba Scutellariae Barbatae, Radix Coicis, Herba corydalis edulis, Fructus Fici and Herba Artemisiae Scopariae are cleaned, put into container, add the distilled water of 8��10 times amount, after soaking 5��6 hours, boil 3��4 hours, extract;Again add 6��8 times amount distilled water, boil 2��3 hours, extract; Finally, add 4��6 times amount distilled water, heated and boiled 1��2 hour, extract; Merge three extracting solution, filter, obtain filtrate, standby;
(2) Radix et Rhizoma Dysosmatis, Herba Ardisiae Maculosae and Radix et Caulis Opuntiae Dillenii are cleaned, immerse 5 times amount 80% alcohol solution dipping 1��2 hour, heating extraction 3 hours, reclaim ethanol, filter, obtain filtrate, concentration is that at 65 DEG C, relative density is the thick paste of 1.12��1.16, standby;
(3) Ramulus Broussonetiae, Amaranthus mangostanus L. and Folium Pruni pseudocerasi are cleaned, chopping, put into container, twist juice, filter impurity, stay juice standby;
(4) Herba Astragali Melilotoidis (Herba Astragali Sinici) and Semen Arecae are put into container, carry out pulverization process, grind into powder, obtain the fine powder of 200 orders, standby;
(5) after the filtrate obtained by above-mentioned steps (1)��(4), thick paste, juice and fine powder being mixed, add the distilled water of 10��12 times amount, after stirring and dissolving is uniform, heated and boiled 0.5 hour, to less than 60 DEG C, stand 3 hours, filter, obtain filtrate, filtrate fill is sealed up for safekeeping, 121 DEG C of pressure sterilizings 20 minutes, packaging, it is described clinical laboratory's disinfection solution.
CN201610106549.8A 2016-02-27 2016-02-27 Sterilization disinfectant for clinical laboratory and preparation method Pending CN105641552A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114984029A (en) * 2022-06-28 2022-09-02 中国疾病预防控制中心病毒病预防控制所 Application of astragalin in preparation of anti-influenza virus medicine

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103705649A (en) * 2014-01-11 2014-04-09 孙永丽 Sterilization disinfectant used for clinical laboratory and preparation method of sterilization disinfectant
CN105123816A (en) * 2015-10-20 2015-12-09 王明儒 Sterilizing and disinfecting liquid for clinical laboratory and preparation method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103705649A (en) * 2014-01-11 2014-04-09 孙永丽 Sterilization disinfectant used for clinical laboratory and preparation method of sterilization disinfectant
CN105123816A (en) * 2015-10-20 2015-12-09 王明儒 Sterilizing and disinfecting liquid for clinical laboratory and preparation method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114984029A (en) * 2022-06-28 2022-09-02 中国疾病预防控制中心病毒病预防控制所 Application of astragalin in preparation of anti-influenza virus medicine

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