CN105616894A - Bactericidal inflammation-diminishing traditional Chinese medicine preparation for laboratory medicine and preparation method thereof - Google Patents

Bactericidal inflammation-diminishing traditional Chinese medicine preparation for laboratory medicine and preparation method thereof Download PDF

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CN105616894A
CN105616894A CN201610068276.2A CN201610068276A CN105616894A CN 105616894 A CN105616894 A CN 105616894A CN 201610068276 A CN201610068276 A CN 201610068276A CN 105616894 A CN105616894 A CN 105616894A
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李衍森
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Abstract

The invention discloses a bactericidal inflammation-diminishing traditional Chinese medicine preparation for laboratory medicine and a preparation method thereof and belongs to the field of traditional Chinese medicine. The traditional Chinese medicine preparation is composed of Areca peel, feather-leaf rodgersflower, Cortex cinnamomi burmannii, leaf of Acronychia pedunculata, Herba cirsii japonici, incised spleenwort, wax gourd, selfheal, mysorethorn, cherry leaf, Herba agrimoniae, Ligustrum sinense, amaranth, leaf of common holly, allium macrostemon and shrubby breynia. Medicinal materials in the traditional Chinese medicine preparation are compatible and cooperative, so that the traditional Chinese medicine preparation has the advantages of resisting bacteria, diminishing inflammation, removing toxicity, subsiding swelling, clearing heat and stopping pain. The traditional Chinese medicine preparation is safe and reliable to use, free of toxic and side effect and irritation to skin and capable of killing bacteria reproductive bodies, fungi, protozoa and part of viruses, can be used for sterilizing skin and mucosa and is worthy of wide popularization and application in the laboratory medicine.

Description

The Chinese medicine preparation of a kind of clinical laboratory bactericidal antiphlogistic and preparation method
Art
The present invention relates to the field of Chinese medicines, particularly relate to Chinese medicine preparation and the preparation method of a kind of clinical laboratory bactericidal antiphlogistic.
Background technology
Clinical laboratory is the bridge between clinical medicine and preclinical medicine, the subdiscipline such as including clinical chemistry, clinical microbiology, clinical immunology, hematology, osology and transfusion science. The section office that clinical laboratory chemically examines as hospital inspection; often some traumatic patients are extracted specimen to chemically examine; and bactericidal antiphlogistic medicine such as iodine tincture, ethanol, povidone iodine etc. that assay office uses are made up of chemicals mostly; human body wound and skin are had serious zest by the chemicals of this kind; it is unfavorable for the healing of wound wound; what have even can cause serious anaphylaxis; if bactericidal antiphlogistic is not thorough; it is easy to fester a wound, can have a strong impact on, sb.'s illness took a favorable turn and lives normally to incur loss through delay patient. So, for providing safety assurance to traumatic patient, it is necessary to find that a kind of bactericidal anti-inflammatory effect is good and Chinese medicine preparation to no skin irritation, solve the misery of patient.
Summary of the invention
The invention provides the Chinese medicine preparation of a kind of clinical laboratory bactericidal antiphlogistic and preparation method; Selected by Chinese medicine preparation of the present invention, medical material compatibility is suitable, all medicine phases and the advantage with anti-inflammation, removing toxic substances and promoting subsidence of swelling, clearing away heat to alleviate pain, and it is safe and reliable, have no side effect, skin is non-stimulated, bacterial propagule, fungus, protozoon and fractionated viral can be killed, it is also possible in the sterilization of skin and mucosa, be worth clinical laboratory's wide popularization and application.
For achieving the above object, technical scheme is implemented as follows:
A kind of Chinese medicine preparation of clinical laboratory bactericidal antiphlogistic, make described Chinese medicine preparation effective ingredient raw material composition and parts by weight be:
34��58 portions of Rhizoma Rodgersiae of Pericarpium Arecae, 32��56 parts of Folium Phyllanthi of 33��57 parts of Cortex Cinnamomi Burmanniis 32��56 parts
31��55 parts of Herba Asplenii Incisis of Radix Cirsii Japonici, 30��54 parts of Spica Prunellaes of 31��55 portions of Fructus Benincasaes 29��53 parts
29��53 parts of Folium Pruni pseudocerasis of Semen Caesalpiniae, 27��51 parts of ligustrum sinenses of 28��52 parts of Herba Agrimoniaes 27��51 parts
27��51 parts of Folium Machili yunnanensiss of Amaranthus mangostanus L., 23��47 parts of Folium et cacumen Breyniae Fruticosae of 26��49 parts of Bulbus Allii Macrostemonis 23��47 parts.
Make described Chinese medicine preparation effective ingredient raw material composition and parts by weight be:
38��54 portions of Rhizoma Rodgersiae of Pericarpium Arecae, 36��52 parts of Folium Phyllanthi of 37��53 parts of Cortex Cinnamomi Burmanniis 36��52 parts
35��51 parts of Herba Asplenii Incisis of Radix Cirsii Japonici, 34��50 parts of Spica Prunellaes of 35��51 portions of Fructus Benincasaes 33��49 parts
33��49 parts of Folium Pruni pseudocerasis of Semen Caesalpiniae, 31��47 parts of ligustrum sinenses of 32��48 parts of Herba Agrimoniaes 31��47 parts
31��47 parts of Folium Machili yunnanensiss of Amaranthus mangostanus L., 27��43 parts of Folium et cacumen Breyniae Fruticosae of 29��45 parts of Bulbus Allii Macrostemonis 27��43 parts.
Make described Chinese medicine preparation effective ingredient raw material composition and parts by weight be:
42��50 portions of Rhizoma Rodgersiae of Pericarpium Arecae, 40��48 parts of Folium Phyllanthi of 41��49 parts of Cortex Cinnamomi Burmanniis 40��48 parts
39��47 parts of Herba Asplenii Incisis of Radix Cirsii Japonici, 38��46 parts of Spica Prunellaes of 39��47 portions of Fructus Benincasaes 37��45 parts
37��45 parts of Folium Pruni pseudocerasis of Semen Caesalpiniae, 35��43 parts of ligustrum sinenses of 36��44 parts of Herba Agrimoniaes 35��43 parts
35��43 parts of Folium Machili yunnanensiss of Amaranthus mangostanus L., 31��39 parts of Folium et cacumen Breyniae Fruticosae of 33��41 parts of Bulbus Allii Macrostemonis 31��39 parts.
Make described Chinese medicine preparation effective ingredient raw material composition and parts by weight be:
46 portions of Rhizoma Rodgersiae of Pericarpium Arecae, 44 parts of Folium Phyllanthi of 45 parts of Cortex Cinnamomi Burmanniis 44 parts
43 parts of Herba Asplenii Incisis of Radix Cirsii Japonici, 42 parts of Spica Prunellaes of 43 portions of Fructus Benincasaes 41 parts
41 parts of Folium Pruni pseudocerasis of Semen Caesalpiniae, 39 parts of ligustrum sinenses of 40 parts of Herba Agrimoniaes 39 parts
39 parts of Folium Machili yunnanensiss of Amaranthus mangostanus L., 35 parts of Folium et cacumen Breyniae Fruticosae of 37 parts of Bulbus Allii Macrostemonis 35 parts.
The preparation method of the Chinese medicine preparation of a kind of clinical laboratory bactericidal antiphlogistic, comprises the following steps that:
(1) Pericarpium Arecae, Folium Phyllanthi, Spica Prunellae, Semen Caesalpiniae, Herba Agrimoniae and ligustrum sinense are cleaned, put into container, add the distilled water of 8��10 times amount, after soaking 5��6 hours, boil 3��4 hours, extract; Again add 6��8 times amount distilled water, boil 2��3 hours, extract; Finally, add 4��6 times amount distilled water, heated and boiled 1��2 hour, extract; Merge three extracting solution, filter, obtain filtrate, standby;
(2) Rhizoma Rodgersiae, Cortex Cinnamomi Burmannii and Folium Machili yunnanensis are put into container, carry out pulverization process, grind into powder, obtain the fine powder of 180 orders, standby;
(3) Radix Cirsii Japonici, Herba Asplenii Incisi, Fructus Benincasae, Folium Pruni pseudocerasi, Amaranthus mangostanus L., Bulbus Allii Macrostemonis and Folium et cacumen Breyniae Fruticosae are cleaned, chopping, put into container, twist juice, filter impurity, stay juice standby;
(4) filtrate made for above-mentioned steps (1)��(3), fine powder and juice are mixed, and add 10-12
The distilled water of times amount, stirring and dissolving uniformly after, heated and boiled 1 hour, to less than 60 DEG C, stands 2 hours, filtration, obtains filtrate, filtrate fill is sealed up for safekeeping, 120 DEG C of pressure sterilizings 20 minutes, obtain the Chinese medicine preparation of described clinical laboratory's bactericidal antiphlogistic.
The property of medicine of Chinese medicine used by the present invention is as follows:
Pericarpium Arecae: acrid in the mouth, slightly warm in nature is nontoxic; Return spleen, lung, stomach warp. Therapeutic method to keep the adverse QI flowing downwards alleviating distention in middle-JIAO, row water. Control gastral cavity abdomen painful abdominal mass swollen, beriberi, edema. " Japan hanako materia medica " carries: " all gas lower, only cholera, logical intestine and small intestine, spleen benefiting and stimulating the appetite, in tune. "
Rhizoma Rodgersiae: bitter in the mouth, puckery, cool in nature. Clearing away heat and cooling blood, menstruction regulating and pain relieving. For enteritis, dysentery, dysmenorrhea, menorrhagia, rheumatic arthritis, traumatic injury; Traumatic hemorrhage, eczema scrotum are controlled in external. " choosing of Yunnan Chinese herbal medicine " carries: " antiinflammatory, convergence, alunite of dispeling the wind, pain relieving. Control knife injury hemorrhage, traumatic injury, rheumatic arthritis, dysentery, diarrhoea. "
Cortex Cinnamomi Burmannii: acrid in the mouth, micro-sweet, warm in nature, return spleen channel. Warming middle-JIAO to relieve pain, expelling wind and cold, removing toxic substances and promoting subsidence of swelling, hemostasis. Curing mainly cold stomachache, stomachache is had loose bowels, inappetence, anemofrigid-damp arthralgia, lumbago and skelalgia, traumatic injury, wound hemorrhage, furuncle toxic swelling. Guangzhou army " conventional Chinese herbal medicine handbook " load: " expelling wind and cold, warming middle-JIAO to relieve pain. Control cold stomachache, abdominal distention, diarrhoea. "
Folium Phyllanthi: sweet in the mouth, micro-hardship, cool in nature; GUIXIN, liver, large intestine channel. Heat-clearing and toxic substances removing, inducing diuresis and reducing edema. Main aphtha, furuncle, eczema, dermatitis, edema, hypertension, venom, traumatic injury. " Luchuan book on Chinese herbal medicine " load: " sterilization removing the necrotic tissue. Control erosion of mucous membrane of the oral cavity, eczema of scrotum and infantile malnutrition, furuncle, leakage, hemorrhoid. "
Radix Cirsii Japonici: sweet in the mouth, hardship, cool in nature, nontoxic; GUIXIN, Liver Channel. Cure mainly haematemesis, epistaxis, hematuria, stranguria with blood, metrorrhagia, leukorrhagia, hemorrhoidal hamorrhage, acute appendicitis, carbuncle and ulcer, toxic swelling, furuncle. " the southern regions of the Yunnan Province book on Chinese herbal medicine " load: " repercussive blood, tissue regeneration promoting blood, emesis blood, nose bleeding. Controlling children's's hematuria, the red hematochezia that collapses of married woman, the raw blood mending all warps, disappear sore, dissipates scrofula tuberculosis, the carbuncle sore person of not closing in for a long time, granulation promoting evacuation of pus. " experimentation proves, Radix Cirsii Japonici has the more significant and lasting large and small Ji of hypotensive activity function all to have heat-clearing and toxic substances removing, antiinflammatory; hemostasis and recover liver function, promote the effect of liver cell regeneration, it is adaptable to acute calenture is struvite hemorrhage, yellow cellulitis; hepatitis, pyretic stranguria, hematuria etc.
Herba Asplenii Incisi: bitter in the mouth, micro-sweet, cool in nature. Heat-clearing and toxic substances removing, cough-relieving sore-throat relieving, inducing diuresis and reducing edema, hemostasis and pain-relieving. For influenza, flu, cough, tonsillitis, parotitis, conjunctival congestion and swelling pain, enteritis, dysentery, breast
Juice not under; The swollen furunculosis of skin ulcer is controlled in external, and traumatic injury, eczema, burn and scald, knife injury is hemorrhage. " conventional glossary of Herbs and Drugs among the people " carries: " controls cough, spits blood, remove lung-heat. "
Fructus Benincasae: sweet in the mouth, light, cold nature; Return lung, intestine and small intestine, bladder warp. Diuresis, heat clearing away, reduce phlegm, promote the production of body fluid, removing toxic substances. Main edema distension, gonorrhea, beriberi, phlegm dyspnea, summer-heat is unhappy, quenches one's thirst, carbuncle, anal fistula, and solves borax poison, ichthyotoxin, alcoholism. " occupying diet spectrum with breath " load: " heat clearing away, nourishing the stomach to promote the production of body fluid, wash dirty control tired, eliminating carbuncle row water, control distension, dysentery cholera, solve the poison such as fish, wine. Also harness the river swollen, relieve summer heat wet. "
Spica Prunellae: bitter in the mouth, pungent, cold in nature, nontoxic, return liver, gallbladder meridian. Clearing away heat-fire, improving eyesight, mass dissipating and swelling eliminating. Cure mainly the diseases such as pestilence, acute mastitis, ophthalmalgia, jaundice, gonorrhea, hypertension. " medicinal herbs among the people of science " carry: " have diuresis bactericidal action. " modern medicine study confirmation, Spica Prunellae decoct can wash wound, controls suppurative outer disease, and can control hypertension, can make blood pressure drops.
Semen Caesalpiniae: bitter in the mouth, puckery, warm in nature, nontoxic. Deliver cold expelling, promoting blood circulation to restore menstrual flow, removing toxic substances parasite killing. For anemofrigid cold, rheumatalgia, traumatic injury, venom. " conventional glossary of Herbs and Drugs among the people " carries: " wind and cold controls cough. Eliminating cold-damp, controls lumbago, toothache, hemorrhoid. " modern medicine study confirmation, Semen Caesalpiniae has cough-relieving, eliminates the phlegm and antiasthmatic effect, and staphylococcus aureus is had bacteriostasis.
Folium Pruni pseudocerasi: sweet-bitter flavor, warm in nature, nontoxic; Return liver, spleen, lung meridian. Warming middle-JIAO and strengthening the spleen, cough-relieving is stopped blooding, and detoxify parasite killing. Main cold syndrome of the stomach food stagnation, diarrhoea, cough, to spit blood, skin infection swells carbuncle, snake bite and insect sting, trichomonas vaginitis. " book on Chinese herbal medicine is new again " carries: " nourishing the liver helps fire, spleen benefiting and stimulating the appetite, and long-pending except gastral cavilty trembles with fear, and helps digestion brokenly stagnant. "
Herba Agrimoniae: bitter in the mouth, puckery, property is put down; Enter lung, liver, spleen channel. Astringing to arrest bleeding, dysentery relieving parasite killing. Cure mainly spitting of blood, spit blood, hematuria, to have blood in stool, dysentery, bleeding not during menses, impairment caused by overstrain takes off power, carbuncle, traumatic injury, wound hemorrhage. " BAICAO mirror " carries: " therapeutic method to keep the adverse QI flowing downwards is invigorated blood circulation, and manages all kinds of diseases and ailments, dissipates feeling of fullness; Fall and flutter haematemesis, metrorrhagia, dysentery, discharging fresh blood stool. " modern medicine study confirms, Herba Agrimoniae is robustness astringent hemostatic, it is suppressed that the activity of plasmin, have heart tonifying, rise blood pressure, blood coagulation, hemostasis, removing heat from blood, the effect such as antibacterial.
Ligustrum sinense: bitter in the mouth, cold in nature. Heat-clearing and toxic substances removing, bacteriostasis and sterilization, reducing swelling and alleviating pain, putrefaction-removing granulation-promoting, for acute icteric infectious hepatitis, dysentery, cough due to lung-heat; The surgical infection diseases such as traumatic injury, traumatic infection, burn and scald, sore swollen toxin are controlled in external.
Amaranthus mangostanus L.: sweet in the mouth, cold nature; Enter large intestine, small intestine meridian. Heat-clearing and toxic substances removing, smoothening secretion. Cure mainly dysentery,
Constipation and anuresis, snakeworm sting, sore. " the southern regions of the Yunnan Province book on Chinese herbal medicine " load: " control sphincter disturbance, change worm cold removing heat, energy promoting blood circulation, removing blood stasis blood. " modern medicine study confirmation, staphylococcus aureus, Staphylococcus albus, Streptococcus viridans, colibacillus, Pseudomonas aeruginosa and klebsiella bacillus are had stronger antibacterial action by the nalka hydro carbons in Amaranthus mangostanus L. extract, nalka alcohols, sterols.
Folium Machili yunnanensis: bitter in the mouth, puckery, cool in nature; GUIXIN, liver, lung, kidney channel. Heat clearing and inflammation relieving. Control parotitis, sore, burn due to hot liquid or fire, rheumatism, injury and bone fracture.
Bulbus Allii Macrostemonis: acrid in the mouth, hardship, warm in nature; Return lung, the heart, stomach, large intestine channel. Regulate the flow of vital energy the chest stuffiness relieving, activating YANG and eliminating stagnation. Curing mainly the thorough back of the body of obstruction of qi in the chest and cardialgia, breast gastral cavity painful abdominal mass is vexed; Cough with asthma abundant expectoration, epigastric pain, heavy after letting out dysentery, leucorrhea, furuncle carbuncle. Modern medicine study confirms, dysentery bacterium, staphylococcus aureus are had inhibitory action by Bulbus Allii Macrostemonis water decoction, 300% water decoction tube dilution method, and staphylococcus aureus, streptococcus pneumoniae are had inhibitory action by 1:4, during 1:16, sarcina are had inhibitory action.
Folium et cacumen Breyniae Fruticosae: mildly bitter flavor, cool in nature. Heat-clearing and toxic substances removing, dissipating blood stasis, pain relieving, antipruritic. Root: acute gastroenteritis, tonsillitis, bronchitis, lithangiuria, puerperal uterine contraction pain, rheumatic arthritis. Leaf: burn and scald, eczema, allergic dermatitis, skin itching, vaginitis are controlled in external. " Luchuan book on Chinese herbal medicine " load: " heat-clearing and toxic substances removing, antidiarrheal, removing mass. Control abdominal pain due to damp-heat, diarrhoea, the arthroncus of knee, treating swelling and pain by traumatic injury. "
The present invention compared with prior art, has the advantages that
Selected by Chinese medicine preparation of the present invention, medical material compatibility is suitable, all medicine phases and the advantage with anti-inflammation, removing toxic substances and promoting subsidence of swelling, clearing away heat to alleviate pain, and it is safe and reliable, have no side effect, skin is non-stimulated, bacterial propagule, fungus, protozoon and fractionated viral can be killed, it is also possible in the sterilization of skin and mucosa, be worth clinical laboratory's wide popularization and application.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further elaborated.
Embodiment 1
The raw material of Chinese medicine preparation effective ingredient of the present invention forms and weight is:
Pericarpium Arecae 54g Rhizoma Rodgersiae 53g Cortex Cinnamomi Burmannii 52g Folium Phyllanthi 52g
Radix Cirsii Japonici 51g Herba Asplenii Incisi 51g Fructus Benincasae 50g Spica Prunellae 49g
Semen Caesalpiniae 49g Folium Pruni pseudocerasi 48g Herba Agrimoniae 47g ligustrum sinense 47g
Amaranthus mangostanus L. 47g Folium Machili yunnanensis 45g Bulbus Allii Macrostemonis 43g Folium et cacumen Breyniae Fruticosae 43g.
The preparation method of the Chinese medicine preparation of a kind of clinical laboratory bactericidal antiphlogistic, comprises the following steps that:
(1) Pericarpium Arecae, Folium Phyllanthi, Spica Prunellae, Semen Caesalpiniae, Herba Agrimoniae and ligustrum sinense are cleaned, put into container, add the distilled water of 8��10 times amount, after soaking 5��6 hours, boil 3��4 hours, extract; Again add 6��8 times amount distilled water, boil 2��3 hours, extract; Finally, add 4��6 times amount distilled water, heated and boiled 1��2 hour, extract; Merge three extracting solution, filter, obtain filtrate, standby;
(2) Rhizoma Rodgersiae, Cortex Cinnamomi Burmannii and Folium Machili yunnanensis are put into container, carry out pulverization process, grind into powder, obtain the fine powder of 180 orders, standby;
(3) Radix Cirsii Japonici, Herba Asplenii Incisi, Fructus Benincasae, Folium Pruni pseudocerasi, Amaranthus mangostanus L., Bulbus Allii Macrostemonis and Folium et cacumen Breyniae Fruticosae are cleaned, chopping, put into container, twist juice, filter impurity, stay juice standby;
(4) filtrate made for above-mentioned steps (1)��(3), fine powder and juice are mixed, and add the distilled water of 10-12 times amount, after stirring and dissolving is uniform, heated and boiled 1 hour, to less than 60 DEG C, stand 2 hours, filter, obtain filtrate, filtrate fill is sealed up for safekeeping, 120 DEG C of pressure sterilizings 20 minutes, obtain the Chinese medicine preparation of described clinical laboratory's bactericidal antiphlogistic.
Embodiment 2
The raw material of Chinese medicine preparation effective ingredient of the present invention forms and weight is:
Pericarpium Arecae 50g Rhizoma Rodgersiae 49g Cortex Cinnamomi Burmannii 48g Folium Phyllanthi 48g
Radix Cirsii Japonici 47g Herba Asplenii Incisi 47g Fructus Benincasae 46g Spica Prunellae 45g
Semen Caesalpiniae 45g Folium Pruni pseudocerasi 44g Herba Agrimoniae 43g ligustrum sinense 43g
Amaranthus mangostanus L. 43g Folium Machili yunnanensis 41g Bulbus Allii Macrostemonis 39g Folium et cacumen Breyniae Fruticosae 39g.
The Chinese medicine preparation preparation method processing step of the present embodiment and usage and dosage and embodiment 1 are identical.
Embodiment 3
The raw material of Chinese medicine preparation effective ingredient of the present invention forms and weight is:
Pericarpium Arecae 46g Rhizoma Rodgersiae 45g Cortex Cinnamomi Burmannii 44g Folium Phyllanthi 44g
Radix Cirsii Japonici 43g Herba Asplenii Incisi 43g Fructus Benincasae 42g Spica Prunellae 41g
Semen Caesalpiniae 41g Folium Pruni pseudocerasi 40g Herba Agrimoniae 39g ligustrum sinense 39g
Amaranthus mangostanus L. 39g Folium Machili yunnanensis 37g Bulbus Allii Macrostemonis 35g Folium et cacumen Breyniae Fruticosae 35g.
The Chinese medicine preparation preparation method processing step of the present embodiment and usage and dosage and embodiment 1 are identical.
Embodiment 4
The raw material of Chinese medicine preparation effective ingredient of the present invention forms and weight is:
Pericarpium Arecae 42g Rhizoma Rodgersiae 41g Cortex Cinnamomi Burmannii 40g Folium Phyllanthi 40g
Radix Cirsii Japonici 39g Herba Asplenii Incisi 39g Fructus Benincasae 38g Spica Prunellae 37g
Semen Caesalpiniae 37g Folium Pruni pseudocerasi 36g Herba Agrimoniae 35g ligustrum sinense 35g
Amaranthus mangostanus L. 35g Folium Machili yunnanensis 33g Bulbus Allii Macrostemonis 31g Folium et cacumen Breyniae Fruticosae 31g.
The Chinese medicine preparation preparation method processing step of the present embodiment and usage and dosage and embodiment 1 are identical.
Embodiment 5
The raw material of Chinese medicine preparation effective ingredient of the present invention forms and weight is:
Pericarpium Arecae 38g Rhizoma Rodgersiae 37g Cortex Cinnamomi Burmannii 36g Folium Phyllanthi 36g
Radix Cirsii Japonici 35g Herba Asplenii Incisi 35g Fructus Benincasae 34g Spica Prunellae 33g
Semen Caesalpiniae 33g Folium Pruni pseudocerasi 32g Herba Agrimoniae 31g ligustrum sinense 31g
Amaranthus mangostanus L. 31g Folium Machili yunnanensis 29g Bulbus Allii Macrostemonis 27g Folium et cacumen Breyniae Fruticosae 27g.
The Chinese medicine preparation preparation method processing step of the present embodiment and usage and dosage and embodiment 1 are identical.
Clinical data
Below Chinese medicine preparation of the present invention is carried out bacteriostatic experiment:
1, experimental strain:
Select the strain that laboratory is representative, staphylococcus aureus and diphtheria corynebacterium.
2, bacterium culture medium:
Nutrient agar and nutrient broth medium.
3, experiment classification:
Bacteriostatic experiment divides experimental group and matched group, and experimental group selects Chinese medicine preparation of the present invention, and matched group selects povidone iodine.
4, specific experiment step:
(1) preparation of bacterium solution: above two strain is inoculated in respectively on two agar culture mediums, cultivating 24 hours at 37 DEG C, from above-mentioned agar culture medium, picking colonies typical is inoculated in two fresh nutrient broth mediums respectively respectively, under synthermal, cultivate 19 hours, standby;
(2) take disinfectant of the present invention and each 55ml of povidone iodine, be respectively placed in cut-back tank, it is diluted respectively 30 times, above two diluent solution is split in two containers respectively, is placed under aseptic condition, standby;
(3) with aseptic nutrient broth, the experimental strain that above-mentioned steps (1) is supported 24 hours through nutrition broth cultivation being diluted 50 times respectively, every kind of strain is sub-packed in two culture dishs, respectively labelling matched group and experimental group;
(4) dilute solution of two parts of Chinese medicine preparation of the present invention of above-mentioned steps (2) gained being respectively dropped into experimental group culture dish, two parts of povidone iodine dilute solutions are respectively dropped into matched group culture dish.
5, experimental observation is measured:
Above-mentioned experiment is observed, observes two groups of strains and measure strain revolution mark concrete outcome.
6, after observation is measured, above-mentioned culture dish is placed under aseptic condition and stands two days, after liquid to be sterilized volatilizees completely, follow the tracks of it has been observed that matched group bacterium colony continues breeding, and experimental group bacterium colony is unchanged.
7, experimental result:
The sterilizing ability of experimental group Chinese medicine preparation of the present invention is much larger than the sterilizing ability of matched group disinfectant solution, and all kinds of strain can be suppressed to continue breeding.

Claims (5)

1. the Chinese medicine preparation of clinical laboratory's bactericidal antiphlogistic, it is characterised in that: make described Chinese medicine preparation effective ingredient raw material composition and parts by weight be:
34��58 portions of Rhizoma Rodgersiae of Pericarpium Arecae, 32��56 parts of Folium Phyllanthi of 33��57 parts of Cortex Cinnamomi Burmanniis 32��56 parts
31��55 parts of Herba Asplenii Incisis of Radix Cirsii Japonici, 30��54 parts of Spica Prunellaes of 31��55 portions of Fructus Benincasaes 29��53 parts
29��53 parts of Folium Pruni pseudocerasis of Semen Caesalpiniae, 27��51 parts of ligustrum sinenses of 28��52 parts of Herba Agrimoniaes 27��51 parts
27��51 parts of Folium Machili yunnanensiss of Amaranthus mangostanus L., 23��47 parts of Folium et cacumen Breyniae Fruticosae of 26��49 parts of Bulbus Allii Macrostemonis 23��47 parts.
2. the Chinese medicine preparation of a kind of clinical laboratory according to claim 1 bactericidal antiphlogistic, it is characterised in that: make described Chinese medicine preparation effective ingredient raw material composition and parts by weight be:
38��54 portions of Rhizoma Rodgersiae of Pericarpium Arecae, 36��52 parts of Folium Phyllanthi of 37��53 parts of Cortex Cinnamomi Burmanniis 36��52 parts
35��51 parts of Herba Asplenii Incisis of Radix Cirsii Japonici, 34��50 parts of Spica Prunellaes of 35��51 portions of Fructus Benincasaes 33��49 parts
33��49 parts of Folium Pruni pseudocerasis of Semen Caesalpiniae, 31��47 parts of ligustrum sinenses of 32��48 parts of Herba Agrimoniaes 31��47 parts
31��47 parts of Folium Machili yunnanensiss of Amaranthus mangostanus L., 27��43 parts of Folium et cacumen Breyniae Fruticosae of 29��45 parts of Bulbus Allii Macrostemonis 27��43 parts.
3. the Chinese medicine preparation of a kind of clinical laboratory according to claim 1 bactericidal antiphlogistic, it is characterised in that: make described Chinese medicine preparation effective ingredient raw material composition and parts by weight be:
42��50 portions of Rhizoma Rodgersiae of Pericarpium Arecae, 40��48 parts of Folium Phyllanthi of 41��49 parts of Cortex Cinnamomi Burmanniis 40��48 parts
39��47 parts of Herba Asplenii Incisis of Radix Cirsii Japonici, 38��46 parts of Spica Prunellaes of 39��47 portions of Fructus Benincasaes 37��45 parts
37��45 parts of Folium Pruni pseudocerasis of Semen Caesalpiniae, 35��43 parts of ligustrum sinenses of 36��44 parts of Herba Agrimoniaes 35��43 parts
35��43 parts of Folium Machili yunnanensiss of Amaranthus mangostanus L., 31��39 parts of Folium et cacumen Breyniae Fruticosae of 33��41 parts of Bulbus Allii Macrostemonis 31��39 parts.
4. the Chinese medicine preparation of a kind of clinical laboratory according to claim 1 bactericidal antiphlogistic, it is characterised in that: make described Chinese medicine preparation effective ingredient raw material composition and parts by weight be:
46 portions of Rhizoma Rodgersiae of Pericarpium Arecae, 44 parts of Folium Phyllanthi of 45 parts of Cortex Cinnamomi Burmanniis 44 parts
43 parts of Herba Asplenii Incisis of Radix Cirsii Japonici, 42 parts of Spica Prunellaes of 43 portions of Fructus Benincasaes 41 parts
41 parts of Folium Pruni pseudocerasis of Semen Caesalpiniae, 39 parts of ligustrum sinenses of 40 parts of Herba Agrimoniaes 39 parts
39 parts of Folium Machili yunnanensiss of Amaranthus mangostanus L., 35 parts of Folium et cacumen Breyniae Fruticosae of 37 parts of Bulbus Allii Macrostemonis 35 parts.
5. the preparation method of the Chinese medicine preparation of clinical laboratory's bactericidal antiphlogistic as described in any one of Claims 1 to 4, it is characterised in that: comprise the following steps that:
(1) Pericarpium Arecae, Folium Phyllanthi, Spica Prunellae, Semen Caesalpiniae, Herba Agrimoniae and ligustrum sinense are cleaned, put into container, add the distilled water of 8��10 times amount, after soaking 5��6 hours, boil 3��4 hours, extract; Again add 6��8 times amount distilled water, boil 2��3 hours, extract; Finally, add 4��6 times amount distilled water, heated and boiled 1��2 hour, extract; Merge three extracting solution, filter, obtain filtrate, standby;
(2) Rhizoma Rodgersiae, Cortex Cinnamomi Burmannii and Folium Machili yunnanensis are put into container, carry out pulverization process, grind into powder, obtain the fine powder of 180 orders, standby;
(3) Radix Cirsii Japonici, Herba Asplenii Incisi, Fructus Benincasae, Folium Pruni pseudocerasi, Amaranthus mangostanus L., Bulbus Allii Macrostemonis and Folium et cacumen Breyniae Fruticosae are cleaned, chopping, put into container, twist juice, filter impurity, stay juice standby;
(4) filtrate made for above-mentioned steps (1)��(3), fine powder and juice are mixed, and add the distilled water of 10-12 times amount, after stirring and dissolving is uniform, heated and boiled 1 hour, to less than 60 DEG C, stand 2 hours, filter, obtain filtrate, filtrate fill is sealed up for safekeeping, 120 DEG C of pressure sterilizings 20 minutes, obtain the Chinese medicine preparation of described clinical laboratory's bactericidal antiphlogistic.
CN201610068276.2A 2016-02-01 2016-02-01 Bactericidal inflammation-diminishing traditional Chinese medicine preparation for laboratory medicine and preparation method thereof Withdrawn CN105616894A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106172547A (en) * 2016-07-30 2016-12-07 张海燕 A kind of operating room disinfection sanitizer and preparation method
CN106234472A (en) * 2016-07-29 2016-12-21 王雪雪 A kind of operating room disinfection sanitizer and preparation method
CN106727142A (en) * 2016-12-29 2017-05-31 周宪伟 A kind of sterilizing hand cleanser of clinical laboratory personnel

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103385260A (en) * 2013-08-05 2013-11-13 王玉芬 Bactericidal medicine for clinical laboratory and preparation method
CN104258347A (en) * 2014-10-11 2015-01-07 王孝芹 Sterilizing and inflammation-diminishing liquid for clinical laboratory

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103385260A (en) * 2013-08-05 2013-11-13 王玉芬 Bactericidal medicine for clinical laboratory and preparation method
CN104258347A (en) * 2014-10-11 2015-01-07 王孝芹 Sterilizing and inflammation-diminishing liquid for clinical laboratory

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106234472A (en) * 2016-07-29 2016-12-21 王雪雪 A kind of operating room disinfection sanitizer and preparation method
CN106172547A (en) * 2016-07-30 2016-12-07 张海燕 A kind of operating room disinfection sanitizer and preparation method
CN106727142A (en) * 2016-12-29 2017-05-31 周宪伟 A kind of sterilizing hand cleanser of clinical laboratory personnel

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