CN105613070A - Method for improving female and male strobilus production of pinus massoniana in seed orchard by aid of cytokinin BAP - Google Patents
Method for improving female and male strobilus production of pinus massoniana in seed orchard by aid of cytokinin BAP Download PDFInfo
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- CN105613070A CN105613070A CN201510968596.9A CN201510968596A CN105613070A CN 105613070 A CN105613070 A CN 105613070A CN 201510968596 A CN201510968596 A CN 201510968596A CN 105613070 A CN105613070 A CN 105613070A
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- pinus massoniana
- bap
- production
- strobilus
- massoniana lamb
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for improving female and male strobilus production of pinus massoniana in a seed orchard by aid of cytokinin BAP and belongs to the field of plant growth regulators. The method comprises steps as follows: a BAP solution is selected as a chemical reagent; 500 mg/L of the BAP solution is prepared, and plants which have blooming and fruiting capability and are 11 years old or older are selected; in the flower bud differentiation period of the pinus massoniana in August and September, 500 mg/L of the BAP solution is injected into terminal buds of pinus massoniana branches by an injector after 2 pm on a sunny day. The problem of low strobilus production of the pinus massoniana in the seed orchard is solved, the female strobilus production of the pinus massoniana can be increased to be 1.75 times of that in a contrast group, and the male strobilus production of the pinus massoniana is increased to be 4.3 times of that in a contrast group.
Description
Technical field
The invention belongs to plant growth regulator, concretely relate to a kind of basic element of cell division BAP method improving breeding garden horse hair Flos pini amount.
Background technology
Pinus massoniana Lamb (Pinusmassoniana) belongs to Pinaceae (Pinaceae), Pinus (Pinus), double; two vascular bundle pine subgenus (Subgen.Pinus), aiphyllium, flower unisexuality, hermaphroditism, florescence early March is to mid-April, anemophily cross pollination. Pinus massoniana Lamb is one of distinctive most important industrial cut stock seeds of China, it is distributed widely on the south the Qinling Mountains, Huaihe River, 17 provinces, autonomous regions and municipalities to the east of the Yunnan-Guizhou Plateau, are be distributed the indigenous tree the widest, area tree comprehensive utilization degree maximum, of many uses, complete is the highest in coniferous species. Pinus massoniana Lamb distribution is wide, strong adaptability, and easily, rapidly, the productivity is high in growth in breeding; After standing forest maturation, before cutting down can resin tapping, cut down rear Herb all available, be the important merchantable timber of China, be again essential industry raw material. Pinus massoniana Lamb still develops the typical seeds of comprehensive utilization, and its economic benefit is significantly high. Pinus massoniana Lamb log is after preservative treatment, and optimum does mine timber, sleeper, electric pole etc., the raw material of the bulky construction such as Ye Shi factory, warehouse, bridge. Pinus massoniana Lamb Ye Shi China mainly produces fat seeds, and Pinus massoniana Lamb Colophonium is one of large outlet forestry products of China. The timber of Pinus massoniana Lamb also has the feature such as content of cellulose height, fiber length, is the pulping and paper-making raw material of high-quality, is suitable for frame news paper, kraft paper and other high-grade paper. Pinus massoniana Lamb is the different flower of hermaphroditism, its breeding mainly seminal propagation the same as most of conifer seeds, and masson pine seed to be the egagametophyte that the male gametophyte produced by Pinus massoniana Lamb male cone produces with Pinus massoniana Lamb female cone be combined, grow procreation through syngenesis and form. Breeding garden is the main base that Pinus massoniana Lamb breeding produces, and flower amount is the major reason causing Pinus Massoniana Lamb In Seed Orchard to yield poorly very little. Improve Pinus Massoniana Lamb In Seed Orchard product quantifier elimination a lot, but current induction by exogenous hormone improves horse hair Flos pini amount to reach the little of effect of high-yield, for this reason, it may be necessary to find effective hormone treatment method, to promote Pinus Massoniana Lamb In Seed Orchard flower amount, and then improve breeding garden yield.
Summary of the invention
It is an object of the invention to provide a kind of basic element of cell division BAP method improving breeding garden horse hair Flos pini amount.
Research according to inventor, it has been found that various plants growth regulator has the ability improving Pinus massoniana Lamb Female and male flowers strobilus production, and wherein basic element of cell division BAP is the one that wherein effect is best.
Technical scheme, including the selection of auxin, the preparation of auxin, auxin solution three steps of injection. Concrete scheme technology is as follows:
Selecting basic element of cell division BAP, chemical name: 6-benzyl aminoadenine, molecular formula is C12H11N5; Basic element of cell division BAP has efficient, stable, cheap and is prone to the features such as use, thus is widely used. The Main Function of basic element of cell division BAP is the formation promoting bud, it is also possible to callus induction occurs. Can alleviating the aging that plant causes because of Water Stress and high temperature, the long period keeps the life-span of bottle transplanting thing. Its major physiological effect is: promote that the cell division of plant and cell volume expand, promote undifferentiated histo-differentiation, promote germination, induced dormancy bud occurs, suppress or promote the elongation growth of stem, leaf, break apical dominance and promote that lateral bud grows, promote flower_bud formation and bloom, promoting to bear fruit, promote fruit growth, induction tuber character, the allocation and transportation of material and accumulation, it is suppressed that or promote to breathe, promote evaporation and stomatal opening, improve anti-injury ability, it is suppressed that chlorophyllous decomposition Delaying Leaf-Senescence, the activity of promotion or inhibitory enzyme.
Compound method: weigh BAP reagent, is configured to the solution of 500mg/L with distilled water constant volume;
Injecting method: select that same Clonal Growth is good at breeding garden, there is setting ability, carry out agent treated without pest and disease damage, 11 years raw Pinus massoniana Lamb that growing way is consistent; Adopting syringe that the basic element of cell division BAP500mg/L solution newly prepared is infused in the terminal bud of branch, to reach, injection site is micro-has drop to stay to be limited for BAP solution usage; Injection time is at 8-9 month Pinus massoniana Lamb flower bud differentiation period, after fine day 14:00.
The present invention is compared with prior art:
1, the present invention is arranging in multiple Concentraton gradient experimental basis, selects 500mg/L as bud concentration for the treatment of.
2, the present invention adopts the mode that the terminal bud of branch is injected by syringe, female, male cone amount to be respectively increased 1.75 times and 4.3 times of comparison, and HORMONE TREATMENT position is clearer and more definite, has saved medicine.
3, what the present invention processed is the terminal bud of branch, and injection time, at 8-9 month Pinus massoniana Lamb flower bud differentiation period, after fine day 14:00, specify that injection time.
4, the advantage that the present invention is maximum be selected hormone kind and processing method all different, for breeding garden Pinus massoniana Lamb high yield provide multiple choices.
Beneficial effects of the present invention:
1, method is simple, and cost is low, it is possible to be applied in breeding garden production.
2, the horse hair Flos pini amount after field planting 11 years can be effectively improved.
Detailed description of the invention
Below in conjunction with example, the present invention is described in further detail.
The present invention is with the Pinus massoniana Lamb of Guizhou Province's Duyun City Pinus massoniana Lamb clonal seed orchard for experiment material, and this embodiment is provided with the basic element of cell division BAP solution of 3 variable concentrations, using blank as compareing.
Compound method: weigh the basic element of cell division BAP100mg, 250mg, 500mg respectively, dissolves the solution being configured to 100mg/L, 250mg/L, 500mg/L with distilled water. Above reagent is the former medicine that dispatches from the factory, and uses after basic element of cell division BAP is completely dissolved.
Plant is selected: good at the same Clonal Growth of breeding garden, there is setting ability, without pest and disease damage, 11 years raw Pinus massoniana Lamb that growing way is consistent.
Processing method: adopt said method newly to prepare basic element of cell division BAP solution, after basic element of cell division BAP is completely dissolved, adopts syringe that the terminal bud of selected plant end of the branch is injected, and to reach, injection site is micro-has drop to stay to be limited for BAP solution usage; After injection time August 20 (fine day) 14:00.
After basic element of cell division BAP injection, strengthen injected plant is managed and protected work, carry out into and spend dynamic observed and recorded. Pinus massoniana Lamb observed result in florescence such as table 1.
As it can be seen from table 1 1.75 times and 4.3 times that the Pinus massoniana Lamb after processing with the basic element of cell division BAP of 500mg/L is female, male cone amount respectively compares, the raising effect processing it lower than this concentration is slightly worse. For present invention research, the basic element of cell division BAP process of 500mg/L can promote that Pinus massoniana Lamb vegetative bud changes to genital stalen, improves the flower amount of Pinus massoniana Lamb, and the raising for breeding garden yield is laid a good foundation.
The above, it it is only presently preferred embodiments of the present invention, not the present invention is done any pro forma restriction, any without departing from technical solution of the present invention content, according to any simple modification, equivalent variations and modification that above example is made by the technical spirit of the present invention, all still fall within the scope of technical solution of the present invention.
Claims (2)
1. the method that a basic element of cell division BAP improves breeding garden Pinus massoniana Lamb Female and male flowers strobilus production, it is characterized in that: with the basic element of cell division BAP that concentration is 500mg/L, the breeding garden Pinus massoniana Lamb Female and male flowers strobilus production of the plant age of tree more than 11 years is regulated and controled, thus improving the flower amount of Pinus massoniana Lamb, 500mg/L basic element of cell division BAP solution is injected into Pinus massoniana Lamb branch terminal bud for adopting syringe by control methods.
2. the method that basic element of cell division BAP according to claim 1 improves breeding garden horse hair Flos pini amount, it is characterised in that: the time of injection basic element of cell division BAP is at 8-9 month Pinus massoniana Lamb flower bud differentiation period, after the 14:00 of fine day.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510968596.9A CN105613070A (en) | 2015-12-22 | 2015-12-22 | Method for improving female and male strobilus production of pinus massoniana in seed orchard by aid of cytokinin BAP |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510968596.9A CN105613070A (en) | 2015-12-22 | 2015-12-22 | Method for improving female and male strobilus production of pinus massoniana in seed orchard by aid of cytokinin BAP |
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| CN105613070A true CN105613070A (en) | 2016-06-01 |
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| CN201510968596.9A Pending CN105613070A (en) | 2015-12-22 | 2015-12-22 | Method for improving female and male strobilus production of pinus massoniana in seed orchard by aid of cytokinin BAP |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258902A (en) * | 2016-08-31 | 2017-01-04 | 昆明理工大学 | The efficient leaf of a kind of succulent inserts propagation method |
| CN107155784A (en) * | 2017-06-26 | 2017-09-15 | 南京林业大学 | It is a kind of to improve the method for proud big chaenomeles lagenaria Flowering quality |
-
2015
- 2015-12-22 CN CN201510968596.9A patent/CN105613070A/en active Pending
Non-Patent Citations (4)
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| R.P.PHARIS: "用激素刺激针叶树开花结实的研究", 《贵州林业科技》 * |
| 张锐等: "激素处理对促进马尾松种子园球花形成的研究", 《湖北林业科技》 * |
| 张锐等: "马尾松早期促进开花结实方法的研究", 《湖北林业科技》 * |
| 邵苗苗等: "不同激素处理对马尾松花芽分化过程中营养物质含量的影响", 《陕西林业科技》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258902A (en) * | 2016-08-31 | 2017-01-04 | 昆明理工大学 | The efficient leaf of a kind of succulent inserts propagation method |
| CN107155784A (en) * | 2017-06-26 | 2017-09-15 | 南京林业大学 | It is a kind of to improve the method for proud big chaenomeles lagenaria Flowering quality |
| CN107155784B (en) * | 2017-06-26 | 2022-07-01 | 南京林业大学 | Method for improving flowering quality of Aoda chaenomeles speciosa |
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Application publication date: 20160601 |
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