CN105613171A - Method for increasing number of female flowers in Pinus massoniana seed orchard by adopting gibberellin GA3 - Google Patents
Method for increasing number of female flowers in Pinus massoniana seed orchard by adopting gibberellin GA3 Download PDFInfo
- Publication number
- CN105613171A CN105613171A CN201510968581.2A CN201510968581A CN105613171A CN 105613171 A CN105613171 A CN 105613171A CN 201510968581 A CN201510968581 A CN 201510968581A CN 105613171 A CN105613171 A CN 105613171A
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- China
- Prior art keywords
- pinus massoniana
- massoniana lamb
- solution
- gibberellins
- seed orchard
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The invention discloses a method for increasing the number of female flowers in a Pinus massoniana seed orchard by adopting gibberellin GA3. The method comprises steps as follows: selection of a plant hormone, determination of plant hormone concentration and injection of a plant hormone solution. The method is characterized by comprising steps as follows: (1), the plant hormone adopts a gibberellin GA3 reagent; (2), a 100 mg/L solution is prepared from gibberellin GA3; (3), the GA3 solution is injected to terminal buds of plant branches by an injector until the GA3 solution dosage reaches the limit that a few droplets are left at the injected parts; the solution is injected after 14:00 in a sunny day at the Pinus massoniana flower bud differentiation stage from August to September. With the adoption of the method, the problem of the small number of the female flowers in the Pinus massoniana seed orchard is solved, and the number of the Pinus massoniana female flowers is increased to 2.6 times of that of a control group.
Description
Technical field
The invention belongs to plant growth regulator field, concretely relate to gibberellins GA3The method improving Pinus Massoniana Lamb In Seed Orchard female cone amount.
Background technology
Pinus massoniana Lamb (Pinusmassoniana) belongs to Pinaceae (Pinaceae), Pinus (Pinus), double; two vascular bundle pine subgenus (Subgen.Pinus), aiphyllium, flower unisexuality, hermaphroditism, florescence early March is to mid-April, anemophily cross pollination. Pinus massoniana Lamb is one of distinctive most important industrial cut stock seeds of China, it is distributed widely on the south the Qinling Mountains, Huaihe River, 17 provinces, autonomous regions and municipalities to the east of the Yunnan-Guizhou Plateau, are be distributed the indigenous tree the widest, area tree comprehensive utilization degree maximum, of many uses, complete is the highest in coniferous species. Pinus massoniana Lamb distribution is wide, strong adaptability, and easily, rapidly, the productivity is high in growth in breeding; After standing forest maturation, before cutting down can resin tapping, cut down rear Herb all available, be the important merchantable timber of China, be again essential industry raw material. Pinus massoniana Lamb still develops the typical seeds of comprehensive utilization, and its economic benefit is significantly high. Pinus massoniana Lamb log is after preservative treatment, and optimum does mine timber, sleeper, electric pole etc., the raw material of the bulky construction such as Ye Shi factory, warehouse, bridge. Pinus massoniana Lamb Ye Shi China mainly produces fat seeds, and Pinus massoniana Lamb Colophonium is one of large outlet forestry products of China. The timber of Pinus massoniana Lamb also has the feature such as content of cellulose height, fiber length, is the pulping and paper-making raw material of high-quality, is suitable for frame news paper, kraft paper and other high-grade paper. Pinus massoniana Lamb is the different flower of hermaphroditism, its breeding mainly seminal propagation the same as most of conifer seeds, and masson pine seed to be the egagametophyte that the male gametophyte produced by Pinus massoniana Lamb male cone produces with Pinus massoniana Lamb female cone be combined, grow procreation through syngenesis and form. Breeding garden is the main base that Pinus massoniana Lamb breeding produces, and female cone amount is the major reason causing Pinus Massoniana Lamb In Seed Orchard to yield poorly very little. Improve Pinus Massoniana Lamb In Seed Orchard product quantifier elimination a lot, but current induction by the exogenous hormone GA3 of concrete concentration improves Pinus massoniana Lamb female cone amount, reach the little of effect of high-yield, for this, needs find effective hormone treatment method, to promote Pinus Massoniana Lamb In Seed Orchard female cone amount, and then improve breeding garden yield.
Summary of the invention
Technical scheme, including the selection of auxin, the preparation of auxin, auxin solution three steps of injection. Concrete scheme technology is as follows:
It is an object of the invention to find new Pinus massoniana Lamb female cone amount is had regulating and controlling effect, and be to increase the plant growth regulator of female cone amount.
Gibberellins GA3, molecular formula: C19H22O6; Gibberellin GA3Have efficient, stable, cheap and be prone to the features such as use, thus being widely used. Gibberellin GA3Main Function be promote stem elongation, it is possible to promote whole plant growth so that short bunch type Kidney bean become climb climing; And short raw genic mutation type Oryza sativa L., Semen Maydis can look the same with wild type high with Semen Pisi sativi, it is possible to promote intercalary growth; Gibberellin GA3Can breaking dormancy, for the seed that light requirement and low temperature could be sprouted, GA3Can substitute for low temperature, the dormancy of seed is broken in illumination so that it is sprout in the dark; Long day plant bolting and blooming when short day can also be induced; The synthesis of Cereal seed ��-amylase can be induced; Can induced parthenocarpy, accelerate certain plants fruit growth, promote to bear fruit; Leaf area can be expanded, accelerate sprout growth, be conducive to metabolite to accumulate in phloem, activate cambium layer.
The present invention studies discovery, uses gibberellins GA3Injection Pinus massoniana Lamb end of the branch is to improve Pinus massoniana Lamb female cone amount by multifactor impact, such as gibberellin GA3The concentration of solution, the Pinus massoniana Lamb age of tree, processing method, treating capacity, process time etc., the present invention is with reference to background technology, on the basis fixing all the other conditions, to gibberellins GA3Concentration for the treatment of and treating capacity have been studied, its rigid condition includes: test plant selects that the same Clonal Growth of breeding garden is good, has setting ability, without pest and disease damage, 11 years raw Pinus massoniana Lamb that growing way is consistent, injection time is 8-9 month Pinus massoniana Lamb flower bud differentiation period, after fine day 14:00, during injection, to reach, injection site is micro-has drop to stay to be limited for solution usage.
Under these conditions, present invention gibberellins GA3Solution injection Pinus massoniana Lamb changes female cone amount and tests, and test shows: gibberellins GA3May be used for the regulation and control of Pinus massoniana Lamb female cone amount, improve Pinus massoniana Lamb female cone amount.
Under certain condition, gibberellins GA3Female cone amount is had impact, gibberellins GA by the concentration of solution injection3The concentration of injection is 100mg/L, the certain condition of indication is the plant age of tree more than 11 years, processing method is to adopt the syringe terminal bud in plant end of the branch to inject, during injection, to reach, injection site is micro-has drop to stay to be limited for solution usage, injection time at 8-9 month Pinus massoniana Lamb flower bud differentiation period, after fine day 14:00. After injection, strengthen injected plant is managed and protected work.
The present invention is compared with prior art:
1, the present invention is arranging in multiple Concentraton gradient experimental basis, selects 100mg/L as bud concentration for the treatment of, and relatively the concentration in prior art process is low, reduces the High concentration auxin murder by poisoning to bud.
2, the present invention adopts the mode that the terminal bud of selected plant end of the branch is injected by syringe, and while having saved medicine, female cone amount improves 26.2%, and HORMONE TREATMENT position is clearer and more definite.
What 3, the present invention processed is the terminal bud of plant end of the branch, and the time fixes on photosynthesis stronger afternoon after 14:00, specify that injection time.
4, the advantage that the present invention is maximum be selected hormone kind and processing method all different from prior art, for breeding garden Pinus massoniana Lamb high yield provide multiple choices.
Beneficial effects of the present invention:
1, method is simple, and cost is low, it is possible to promote in producing for breeding garden.
2, the Pinus massoniana Lamb Female and male flowers strobilus production after field planting 11 years can be made to improve, the Female and male flowers strobilus production of Pinus massoniana Lamb can be effectively improved.
Detailed description of the invention
Below in conjunction with example, the present invention is described in further detail.
The present invention is with the Pinus massoniana Lamb of Guizhou Province's Duyun City Pinus massoniana Lamb clonal seed orchard for experiment material, and this example is provided with the gibberellin GA of 3 variable concentrations3Solution, using blank as comparison.
Compound method: by gibberellins GA3It is configured to the solution of 100mg/L, 250mg/L, 500mg/L, treats gibberellins GA3Use after being completely dissolved.
Plant is selected: good at the same Clonal Growth of breeding garden, there is setting ability, without pest and disease damage, 11 years raw Pinus massoniana Lamb that growing way is consistent.
Processing method: utilize the gibberellins GA that syringe will newly prepare3Solution injects the terminal bud of selected plant end of the branch, GA3To reach, injection site is micro-has drop to stay to be limited for solution usage; Injection time is after August 20 (fine day) 14:00.
Gibberellins GA3After injection, strengthen inject plant is managed and protected work, it is prevented that domestic animal tramples on or artificial destruction, the emphasis prevention and control of plant diseases, pest control, carry out into and spend dynamic observed and recorded. Pinus massoniana Lamb observed result in florescence such as table 1.
As it can be seen from table 1 with the gibberellins GA of 100mg/L3What the female cone amount after process relatively compareed improves 2.6 times, for present invention research, after HORMONE TREATMENT, improves the yield of female cone, lays the foundation for breeding garden high yield.
The above, it it is only presently preferred embodiments of the present invention, not the present invention is done any pro forma restriction, any without departing from technical solution of the present invention content, according to any simple modification, equivalent variations and modification that above example is made by the technical spirit of the present invention, all still fall within the scope of technical solution of the present invention.
Claims (3)
1. a gibberellins GA3The method improving Pinus Massoniana Lamb In Seed Orchard female cone amount, it is characterised in that: the gibberellins GA adopting concentration to be 100mg/L3Pinus Massoniana Lamb In Seed Orchard female cone amount is regulated and controled by phytohormone solution, and to reach to improve the purpose of Pinus Massoniana Lamb In Seed Orchard female cone amount, control methods are to adopt syringe by 100mg/L gibberellins GA3Solution is injected into Pinus massoniana Lamb branch terminal bud.
2. gibberellins GA according to claim 13The method improving Pinus Massoniana Lamb In Seed Orchard female cone amount, it is characterised in that: gibberellins GA3To reach, injection site is micro-has drop to stay to be limited for phytohormone solution usage.
3. gibberellins GA according to claim 13The method improving Pinus Massoniana Lamb In Seed Orchard female cone amount, it is characterised in that: injection gibberellins GA3The time of phytohormone solution is at 8-9 month Pinus massoniana Lamb flower bud differentiation period, after fine day 14:00.
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| CN201510968581.2A CN105613171A (en) | 2015-12-22 | 2015-12-22 | Method for increasing number of female flowers in Pinus massoniana seed orchard by adopting gibberellin GA3 |
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| CN201510968581.2A CN105613171A (en) | 2015-12-22 | 2015-12-22 | Method for increasing number of female flowers in Pinus massoniana seed orchard by adopting gibberellin GA3 |
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| CN105613171A true CN105613171A (en) | 2016-06-01 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114503988A (en) * | 2022-02-18 | 2022-05-17 | 东北林业大学 | Exogenous hormone for promoting clonal female flower quantity of Korean pine |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103596422A (en) * | 2011-06-30 | 2014-02-19 | 韦尔豪泽Nr公司 | Methods for modulating strobili development in gymnosperm trees |
-
2015
- 2015-12-22 CN CN201510968581.2A patent/CN105613171A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103596422A (en) * | 2011-06-30 | 2014-02-19 | 韦尔豪泽Nr公司 | Methods for modulating strobili development in gymnosperm trees |
Non-Patent Citations (3)
| Title |
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| R.P.PHARIS: "用激素刺激针叶树开花结实的研究", 《贵州林业科技》 * |
| 江玲等: "马尾松雌雄球花的形成与赤霉素和脱落酸及细胞分裂素的关系", 《南京林业大学学报》 * |
| 邵苗苗等: "不同激素处理对马尾松花芽分化过程中营养物质含量的影响", 《陕西林业科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114503988A (en) * | 2022-02-18 | 2022-05-17 | 东北林业大学 | Exogenous hormone for promoting clonal female flower quantity of Korean pine |
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