CN102805034A - Method for quickly breeding peanut seedlings by using peanut stems - Google Patents
Method for quickly breeding peanut seedlings by using peanut stems Download PDFInfo
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- CN102805034A CN102805034A CN201210308016XA CN201210308016A CN102805034A CN 102805034 A CN102805034 A CN 102805034A CN 201210308016X A CN201210308016X A CN 201210308016XA CN 201210308016 A CN201210308016 A CN 201210308016A CN 102805034 A CN102805034 A CN 102805034A
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Abstract
The invention provides a method for quickly breeding peanut seedlings by using peanut stems. By the method, the demand of peanut seeds is reduced, and peanut seeds are reduced. According to the method provided by the invention, the peanut seedlings are quickly bred by utilizing waste explants so as to quicken the generalization of good peanut varieties, improve economic efficiency, and quickly reduce the cost in the generalization of the good peanut varieties. The method is particularly designed for the seed breeding of hybridized peanut varieties. The method does not result in the separation of good genes of the hybridized peanut varieties so as to ensure the consistency of the hybridized peanut varieties. By the method for breeding the peanut seedlings, requirements for conditions are low, and equipment investment is low; and the method is easy to control.
Description
Technical field
The present invention relates to technical field of plant culture, specifically a kind of peanut stem section method of breeding fast of utilizing.
Background technology
The reproduction coefficient of peanut is always very low, generally adopts the seed single-plant propagation, and under the cultivation condition of higher level, reproduction coefficient also has only about 50 times.Therefore, the popularization of new peanut improved seeds often will be passed through the long time limit, can not satisfy the needs of production development.
Forefathers utilize the Gao Jiewei flower of peanut to breed the per mu yield that can improve peanut; This culture technique of mainly still improving peanut improves the rate that bears pods of peanut; In the process of growth of peanut, adopt the method for layer, make peanut stem more approach ground, can make Gao Jiewei fruit pin more be prone to bury bear pods.
Publication number has been introduced the method for quickly breeding of cultivating peanut for the CN102283001A Chinese patent; Being employed in annual March and twice type of seeding in June breeds fast; Improve the seed production of good peanut varieties; Owing to the sowing in June needs a large amount of seeds and causes the fruiting of the groundnut rate low because of restricting reason season, the sowing cost of labor increased greatly in other 1 year two, seldom saw this propagation method at present in process of production.
Publication number has been introduced the method for in-vitro rapid propagation of cultivating peanut for the CN101411305B Chinese patent; Adopt the seed or the stem eye of peanut to carry out tissue culture, through enrichment culture, differentiation obtains test-tube plantlet; Carry out field production again, this propagation method can obtain abundant peanut seed in 1 year.But cause peanut variation to occur easily in the plant tissue culture technology; Influenced the popularization of good peanut varieties; The group training need be tested seedling in addition, and test-tube plantlet is prone to death in this process, causes peanut tissue cultivating seedling price very high; Increased the production cost of peanut greatly, the plant tissue culture media composition also can cause the variation of good peanut varieties in addition.
Therefore, in order to satisfy the peanut demand that increases day by day, seed harvest yield is little; The seminal propagation emergence rate is extremely low, and the cultivating seeds process is loaded down with trivial details, and growth rate is slow; Peanut is an annual herb, though its stem section part be prone to take root, but its root system and undeveloped; If utilize the peanut stem section to breed seedling fast, can make the well developed root system of peanut seedling again, be the best solution that realizes that the peanut improved seeds are promoted fast.
Up to now, at home and abroad do not see as yet about the peanut stem section and cultivate fast numerous successful report.We pass through a large amount of tests, find out the ripe method of a cover finally, have realized that successfully the cultivation of peanut stem section is numerous soon, can go out a large amount of seedling by quickly breeding, for realizing that cultivating the peanut improved seeds fast provides possibility.
Summary of the invention
The method for quickly breeding that the purpose of this invention is to provide peanut, this method are fit to the popularization of good peanut varieties very much, and the prescription effect is good, and planting percent is high, and incubation time is short, and plant strain growth is vigorous, and is workable, the using value advantages of higher.Has less investment, the characteristics that output is high.
According to the invention one method for quickly breeding of cultivating peanut, its concrete operations step is following:
(1) choice of base: adopt thin sand and soil to carry out 150cm, 150cm opens the railway carriage or compartment, and the surface is whole carefully rakes;
(2) sterilization of matrix and sterilization: adopt carbendazim solution to spray the wine secondary, each 15 days at interval;
(3) explant selection and processing: select for use the peanut plant of fast harvesting to handle, spray treatment fluid to it and get the peanut stem epimere after 15 days as culture materials;
(4) sterilization of explant: peanut stem section top defoliation is cleaned through liquid detergent and running water, puts under the flowing water and washes after 10-30 minute, places carbendazim solution to handle 30-60 second, and the stem section is cut into the band bud segment of 2-4cm;
(5) culture of rootage: place rooting powder solution to soak 15-20min the band axillalry bud segment after the sterilization processing; Place again on the culture matrix that step (2) handled; Using the 0.01mol/L hydrochloric acid solution to regulate culture matrix pH value is 5.6, cultivation temperature 23-24 ℃, and illumination every day 12 hours; Intensity of illumination 1000LX, humidity remains on about 70%;
(6) final-period management: when the rooting rate of peanut reaches more than 90%; If be in the season of growth that is fit to peanut; Can direct transplanting to the land for growing field crops, if be in the season of growth that is not suitable for peanut, can reduce about environmental temperature to 10 ℃; After transplanting the land for growing field crops about March, manage by the peanut field production.
In a specific embodiments, carbendazim solution described in the step (2), its concentration is 1-5g/300mL.
In a specific embodiments, treatment fluid described in the step (3), its treatment fluid comprises the aqueous solution of 5-10g/L root-inducing powder and 10-20g/L urea.
In a specific embodiments, carbendazim solution described in the step (4), its concentration is 1g/500mL.
In another embodiment, rooting powder solution described in the step (5), its concentration is 1g-3g/100mL.
Technique effect
(1) utilizes the peanut stem section to breed the demand that has reduced peanut seed fast, saved peanut seed.
(2) the discarded peanut stem explants of the inventive method utilization has been set up quick breeding peanut seedling, has quickened the promotion rate of peanut improved seeds, has improved its economic benefit, can reduce the popularization cost of improved seeds fast.
(3) to the seminal propagation of hybridization peanut, the excellent genes that can not cause hybrid seed to occur through the method separates the inventive method, can guarantee the uniformity of hybridization peanut seed especially.
(4) condition of these propagation method production peanut seedling needs is simple, easy to control, and investment goods is few.
Embodiment
Below, the present invention will further explain with embodiment, but it is not limited to any or the similar instance of these embodiment.
Embodiment 1
Draw materials: peanut band bud rhizome section is an explant
(1) choice of base: adopt thin sand and soil to carry out 150cm, 150cm opens the railway carriage or compartment, and the surface is whole carefully rakes;
(2) sterilization of matrix and sterilization: adopt the 10g/L carbendazim solution to spray the wine secondary, each 15 days at interval;
(3) explant selection and processing: select for use the peanut plant of peanut production base, Cili, Hunan on the 15th in August to handle, contain the aqueous solution of 5g/L root-inducing powder and 20g/L urea, handle and get the peanut stem epimere as culture materials after 15 days to its sprinkling;
(4) sterilization of explant: peanut stem section top defoliation is cleaned through liquid detergent and running water, puts under the flowing water and washes after 20 minutes, places the 2g/L carbendazim solution to handle 30 seconds, and the stem section is cut into the band bud segment of 2-4cm;
(5) culture of rootage: place the 30g/L rooting powder solution to soak 15min the band axillalry bud segment after the sterilization processing; Place again on the culture matrix that step (2) handled; Using the 0.01mol/L hydrochloric acid solution to regulate culture matrix pH value is 5.6, cultivation temperature 23-24 ℃, and illumination every day 12 hours; Intensity of illumination 1000LX, humidity remains on about 70%;
(6) final-period management: the rooting rate when peanut after month reaches more than 90%, and direct transplanting is carried out Routine Management to the big Tanaka in base, Sanya, Hainan by the peanut field production, reaches more than 95% through the survival rate of adding up peanut.
Control Example
Draw materials: peanut band bud rhizome section is an explant
(1) choice of base: adopt thin sand and soil to carry out 150cm, 150cm opens the railway carriage or compartment, and the surface is whole carefully rakes;
(2) sterilization of matrix and sterilization: adopt the 10g/L carbendazim solution to spray the wine secondary, each 15 days at interval;
(3) explant selection and sterilization: select for use the peanut stem section top defoliation of peanut production base, Cili, Hunan on the 30th in August to clean through liquid detergent and running water; Put and wash after 20 minutes under the flowing water; Place the 2g/L carbendazim solution to handle 30 seconds, the stem section is cut into the band bud segment of 2-4cm;
(4) culture of rootage: place the 30g/L rooting powder solution to soak 15min the band axillalry bud segment after the sterilization processing; Place again on the culture matrix that step (2) handled; Using the 0.01mol/L hydrochloric acid solution to regulate culture matrix pH value is 5.6, cultivation temperature 23-24 ℃, and illumination every day 12 hours; Intensity of illumination 1000LX, humidity remains on about 70%;
(5) final-period management: the rooting rate of peanut reaches about 45% after one month, and direct transplanting is carried out Routine Management to the big Tanaka in base, Sanya, Hainan by the peanut field production, reaches more than 35% through the survival rate of adding up peanut.
Embodiment 2
Draw materials: peanut band bud rhizome section is an explant
(1) choice of base: adopt thin sand and soil to carry out 150cm, 150cm opens the railway carriage or compartment, and the surface is whole carefully rakes;
(2) sterilization of matrix and sterilization: adopt the 14g/L carbendazim solution to spray the wine secondary, each 15 days at interval;
(3) explant selection and processing: select for use the peanut plant of peanut production base, Cili, Hunan on the 15th in August to handle, contain the aqueous solution of 8g/L root-inducing powder and 15g/L urea, handle and get the peanut stem epimere as culture materials after 15 days to its sprinkling;
(4) sterilization of explant: peanut stem section top defoliation is cleaned through liquid detergent and running water, puts under the flowing water and washes after 30 minutes, places the 2g/L carbendazim solution to handle 40 seconds, and the stem section is cut into the band bud segment of 2-4cm;
(5) culture of rootage: place the 20g/L rooting powder solution to soak 25min the band axillalry bud segment after the sterilization processing; Place again on the culture matrix that step (2) handled; Using the 0.01mol/L hydrochloric acid solution to regulate culture matrix pH value is 5.6, cultivation temperature 23-24 ℃, and illumination every day 12 hours; Intensity of illumination 1000LX, humidity remains on about 70%;
(6) final-period management: when the rooting rate of peanut reaches more than 90%, reduce about environmental temperature to 10 ℃, transplant land for growing field crops, peanut base, Cili, Hunan about March after, carry out Routine Management by the peanut field production.
Embodiment 3
Draw materials: peanut band bud rhizome section is an explant
(1) choice of base: adopt thin sand and soil to carry out 150cm, 150cm opens the railway carriage or compartment, and the surface is whole carefully rakes;
(2) sterilization of matrix and sterilization: adopt the 15g/L carbendazim solution to spray the wine secondary, each 15 days at interval;
(3) explant selection and processing: select for use the peanut plant in Sanya, Hainan on the 20th in January peanut breeding base to handle, contain the aqueous solution of 10g/L root-inducing powder and 10g/L urea, handle and get the peanut stem epimere as culture materials after 15 days to its sprinkling;
(4) sterilization of explant: peanut stem section top defoliation is cleaned through liquid detergent and running water, puts under the flowing water and washes after 30 minutes, places the 2g/L carbendazim solution to handle 40 seconds, and the stem section is cut into the band bud segment of 2-4cm;
(5) culture of rootage: place the 20g/L rooting powder solution to soak 30min the band axillalry bud segment after the sterilization processing; Place again on the culture matrix that step (2) handled; Using the 0.01mol/L hydrochloric acid solution to regulate culture matrix pH value is 5.6, cultivation temperature 23-24 ℃, and illumination every day 12 hours; Intensity of illumination 1000LX, humidity remains on about 70%;
(6) final-period management: when the rooting rate of peanut reaches more than 90%, reduce about environmental temperature to 10 ℃, transplant land for growing field crops, peanut base, Xinyang, Henan about May after, carry out Routine Management by the peanut field production.
Claims (5)
1. a kind of peanut stem section of utilizing according to the invention is bred the peanut seedling-growing method fast, and its concrete operations step is following:
(1) choice of base: adopt thin sand and soil to carry out 150cm, 150cm opens the railway carriage or compartment, and the surface is whole carefully rakes;
(2) sterilization of matrix and sterilization: adopt the carbendazim aqueous solution to spray the wine secondary, each 15 days at interval;
(3) explant selection and processing: select for use the peanut plant of fast harvesting to handle, spray treatment fluid to it and get the peanut stem epimere after 15 days as culture materials;
(4) sterilization of explant: peanut stem section top defoliation is cleaned through liquid detergent and running water, puts under the flowing water and washes after 10-30 minute, places carbendazim solution to handle 30-60 second, and the stem section is cut into the band bud segment of 2-4cm;
(5) culture of rootage: place rooting powder solution to soak 15-20min the band axillalry bud segment after the sterilization processing; Place again on the culture matrix that step (2) handled; Using the 0.01mol/L hydrochloric acid solution to regulate culture matrix pH value is 5.6, cultivation temperature 23-24 ℃, and illumination every day 12 hours; Intensity of illumination 1000LX, humidity remains on about 70%;
(6) final-period management: when the rooting rate of peanut reaches more than 90%; If be in the season of growth that is fit to peanut; Can direct transplanting to the land for growing field crops, if be in the season of growth that is not suitable for peanut, can reduce about environmental temperature to 10 ℃; After transplanting the land for growing field crops about March, manage by the peanut field production.
2. according to the method for claim 1, the carbendazim aqueous solution described in the said step (2), its quality and water volume concentration ratio are 1-5g/300mL.
3. according to the method for claim 1, treatment fluid described in the said step (3), its treatment fluid comprises the aqueous solution of 5-10g/L root-inducing powder and 10-20g/L urea, and its concentration is quality and water volume ratio.
4. according to the method for claim 1, the carbendazim aqueous solution described in the said step (4), its quality and water volume concentration ratio are 1g/500mL.
5. according to the method for claim 1, the root-inducing powder aqueous solution described in said rapid (5), its quality and water volume concentration ratio are 1g-3g/100mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201210308016XA CN102805034A (en) | 2012-08-27 | 2012-08-27 | Method for quickly breeding peanut seedlings by using peanut stems |
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| CN201210308016XA CN102805034A (en) | 2012-08-27 | 2012-08-27 | Method for quickly breeding peanut seedlings by using peanut stems |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103733864A (en) * | 2014-01-15 | 2014-04-23 | 张阿兴 | Method for using sweet potato vines to reproduce sweet potato seedlings |
| CN103734011A (en) * | 2013-12-31 | 2014-04-23 | 广西壮族自治区农业科学院经济作物研究所 | Method for promoting overground fruiting of peanut gynophores |
| CN110178559A (en) * | 2019-07-04 | 2019-08-30 | 广西壮族自治区农业科学院 | A method of promoting rhizome district's groups wild-type peanut result |
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| JPH04222525A (en) * | 1990-12-20 | 1992-08-12 | Nippon Unicar Co Ltd | Cultivation of peanut |
| CN101411305A (en) * | 2008-11-25 | 2009-04-22 | 华南师范大学 | Method for quickly reproducing in-vitro pea nut |
| CN101946617A (en) * | 2010-08-23 | 2011-01-19 | 湖南农业大学 | Cottage and rapid propagation method of perennial energy grass |
| CN102283001A (en) * | 2011-06-29 | 2011-12-21 | 山东省花生研究所 | Quick peanut propagation method |
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2012
- 2012-08-27 CN CN201210308016XA patent/CN102805034A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04222525A (en) * | 1990-12-20 | 1992-08-12 | Nippon Unicar Co Ltd | Cultivation of peanut |
| CN101411305A (en) * | 2008-11-25 | 2009-04-22 | 华南师范大学 | Method for quickly reproducing in-vitro pea nut |
| CN101946617A (en) * | 2010-08-23 | 2011-01-19 | 湖南农业大学 | Cottage and rapid propagation method of perennial energy grass |
| CN102283001A (en) * | 2011-06-29 | 2011-12-21 | 山东省花生研究所 | Quick peanut propagation method |
Non-Patent Citations (2)
| Title |
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| 王涛: "《植物扦插繁殖技术》", 28 February 1989 * |
| 董洪章: "ABT生根粉在花生枝条扦插上的应用", 《中国油料》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103734011A (en) * | 2013-12-31 | 2014-04-23 | 广西壮族自治区农业科学院经济作物研究所 | Method for promoting overground fruiting of peanut gynophores |
| CN103734011B (en) * | 2013-12-31 | 2016-07-06 | 广西壮族自治区农业科学院经济作物研究所 | The method promoting peanut pin result on the ground |
| CN103733864A (en) * | 2014-01-15 | 2014-04-23 | 张阿兴 | Method for using sweet potato vines to reproduce sweet potato seedlings |
| CN103733864B (en) * | 2014-01-15 | 2015-05-06 | 张阿兴 | Method for using sweet potato vines to reproduce sweet potato seedlings |
| CN110178559A (en) * | 2019-07-04 | 2019-08-30 | 广西壮族自治区农业科学院 | A method of promoting rhizome district's groups wild-type peanut result |
| CN110178559B (en) * | 2019-07-04 | 2021-06-15 | 广西壮族自治区农业科学院 | Method for promoting rhizomatic block wild peanut fruiting |
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Application publication date: 20121205 |