CN105531377A - 基于dna的定量成像和超分辨成像 - Google Patents

基于dna的定量成像和超分辨成像 Download PDF

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Publication number
CN105531377A
CN105531377A CN201480048129.8A CN201480048129A CN105531377A CN 105531377 A CN105531377 A CN 105531377A CN 201480048129 A CN201480048129 A CN 201480048129A CN 105531377 A CN105531377 A CN 105531377A
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imaging
chain
mark
protein
drift
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R·容曼
P·尹
M·戴
M·阿文戴诺
J·B·韦尔斯坦恩
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Harvard University
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Harvard University
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/20—Analysis of motion
    • G06T7/246—Analysis of motion using feature-based methods, e.g. the tracking of corners or segments
    • G06T7/248—Analysis of motion using feature-based methods, e.g. the tracking of corners or segments involving reference images or patches
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/10—Signal processing, e.g. from mass spectrometry [MS] or from PCR
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
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    • G16B45/00—ICT specially adapted for bioinformatics-related data visualisation, e.g. displaying of maps or networks
    • C—CHEMISTRY; METALLURGY
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    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies

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  • Bioinformatics & Computational Biology (AREA)
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CN201480048129.8A 2013-07-30 2014-07-30 基于dna的定量成像和超分辨成像 Pending CN105531377A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361859891P 2013-07-30 2013-07-30
US61/859,891 2013-07-30
US201361884126P 2013-09-29 2013-09-29
US61/884,126 2013-09-29
US201461934759P 2014-02-01 2014-02-01
US61/934,759 2014-02-01
PCT/US2014/048977 WO2015017586A1 (en) 2013-07-30 2014-07-30 Quantitative dna-based imaging and super-resolution imaging

Publications (1)

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CN105531377A true CN105531377A (zh) 2016-04-27

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US (2) US20160161472A1 (https=)
EP (2) EP3696277B2 (https=)
JP (1) JP6475718B2 (https=)
KR (1) KR20160039229A (https=)
CN (1) CN105531377A (https=)
AU (1) AU2014296253A1 (https=)
CA (1) CA2919833A1 (https=)
HK (1) HK1221747A1 (https=)
WO (1) WO2015017586A1 (https=)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107894411A (zh) * 2017-10-27 2018-04-10 上海纳米技术及应用国家工程研究中心有限公司 荧光精确定量dna折纸结构完整度的方法
CN107934531A (zh) * 2017-12-25 2018-04-20 福建上源生物科学技术有限公司 一种自动线虫挑选装置
CN109030434A (zh) * 2018-06-20 2018-12-18 天津大学 一种基于Android平台的真菌毒素含量检测方法
CN109071641A (zh) * 2016-04-26 2018-12-21 乌尔蒂维尤股份有限公司 具有衍射极限预览的超分辨率免疫荧光
CN110168346A (zh) * 2016-11-08 2019-08-23 哈佛学院院长及董事 使用merfish、扩展显微术和相关技术进行多重成像
CN110364224A (zh) * 2018-12-29 2019-10-22 上海北昂医药科技股份有限公司 一种染色体分裂相定位排序方法
CN113281320A (zh) * 2021-06-07 2021-08-20 江南大学 一种基于荧光铜纳米粒子检测黄曲霉毒素b1的方法
CN114921537A (zh) * 2017-03-17 2022-08-19 雅普顿生物系统公司 测序和高分辨率成像
WO2022188496A1 (zh) * 2021-03-08 2022-09-15 光华临港工程应用技术研发(上海)有限公司 一种用于膨胀超分辨成像的dna纳米结构染料及其应用
US11788123B2 (en) 2017-05-26 2023-10-17 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
US11959075B2 (en) 2014-07-30 2024-04-16 President And Fellows Of Harvard College Systems and methods for determining nucleic acids
CN119595679A (zh) * 2024-12-16 2025-03-11 四川大学 一种植鞣皮革文物的鉴定方法
US12460250B2 (en) 2018-12-13 2025-11-04 President And Fellows Of Harvard College Amplification methods and systems for MERFISH and other applications

Families Citing this family (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
CN105378477B9 (zh) 2013-03-11 2021-08-06 梅索磅秤技术有限公司 用于进行多路测定法的改进方法
AU2014296253A1 (en) 2013-07-30 2016-02-04 President And Fellows Of Harvard College Quantitative DNA-based imaging and super-resolution imaging
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
US20180224461A1 (en) * 2015-08-07 2018-08-09 President And Fellows Of Harvard College Super resolution imaging of protein-protein interactions
EP3347400A1 (en) 2015-09-10 2018-07-18 King Abdullah University Of Science And Technology Conjugated polymer nanoparticles, methods of using, and methods of making
WO2017049136A1 (en) * 2015-09-18 2017-03-23 President And Fellows Of Harvard College Nucleic acid frameworks for structural determination
WO2017087823A1 (en) 2015-11-18 2017-05-26 Mir Kalim U Super-resolution sequencing
DE102015016353A1 (de) 2015-12-16 2017-06-22 Horst Wochnowski PALM-Verfahren mit mehreren sich nur marginal voneinander unterscheidenden Fluorophoren
CN114196746A (zh) * 2016-02-10 2022-03-18 密歇根大学董事会 核酸的检测
US20190120767A1 (en) * 2016-04-26 2019-04-25 Ultivue, Inc. Super-Resolution Fluorescence Microscopy Method Using Improved Drift Compensation Markers
EP4101932A1 (en) 2016-05-15 2022-12-14 Ultivue, Inc. Multiplexed imaging using strand displacement
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
EP3465162A4 (en) 2016-06-02 2020-03-25 Ultivue, Inc. COMPOSITIONS AND METHODS FOR ACCELERATING ANTIBODY EXCHANGE IMAGING
CN109789228B (zh) * 2016-07-27 2022-10-21 斯坦福大学托管董事会 高度复用荧光成像
EP3290983A1 (de) * 2016-09-05 2018-03-07 Abberior Instruments GmbH Verfahren zum justieren eines laserscanning-fluoreszenzmikroskops und laserscanning-fluoreszenzmikroskop mit einer automatischen justiervorrichtung
JP2020504600A (ja) 2016-12-09 2020-02-13 アルティヴュー， インク． 標識化核酸イメージング剤を使用した多重イメージングのための改善された方法
FI3929304T3 (fi) * 2016-12-16 2024-03-27 Aratome Llc Molekulaarin tunnistaminen käyttämällä ligatiovahvistusta
US10281402B2 (en) * 2017-01-26 2019-05-07 Azure Biosystems, Inc. Devices and methods for imaging biomolecules
DE102017104736B9 (de) * 2017-03-07 2020-06-25 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren und Vorrichtung zum räumlichen Messen nanoskaliger Strukturen
AU2018231211A1 (en) * 2017-03-08 2019-10-03 The Regents Of The University Of Michigan Analyte detection
CN117187346A (zh) * 2017-03-31 2023-12-08 乌尔蒂维尤股份有限公司 Dna-抗原交换和扩增
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
US11331019B2 (en) 2017-08-07 2022-05-17 The Research Foundation For The State University Of New York Nanoparticle sensor having a nanofibrous membrane scaffold
GB2569283B (en) * 2017-12-05 2021-10-13 Continental Automotive Romania Srl Method and device for adaptively enhancing an output of a detector
DK3498865T3 (da) * 2017-12-14 2020-12-21 Univ Muenchen Ludwig Maximilians Påvisning og kvantificering af enkeltmolekyler ved hjælp af DNA-nanoteknologi i microwells
KR20190108023A (ko) * 2018-03-13 2019-09-23 서울대학교산학협력단 초고감도 바이오마커 다중 검출 방법
WO2019196019A1 (zh) * 2018-04-10 2019-10-17 深圳华大智造科技有限公司 荧光图像配准方法、基因测序仪及系统、存储介质
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
WO2020014036A1 (en) * 2018-07-09 2020-01-16 Ultivue, Inc. Methods for multicolor multiplex imaging
EP4242324B1 (en) * 2018-08-17 2025-09-24 Becton, Dickinson and Company Aptamer barcoding
US20230176064A1 (en) * 2018-08-28 2023-06-08 Jl Medilabs, Inc. Methods and kits for detecting target substances
CN109255427A (zh) * 2018-08-30 2019-01-22 无锡城市职业技术学院 一种基于量子粒子群优化算法的分子对接计算方法
CN109371031A (zh) * 2018-11-23 2019-02-22 北京化工大学 一种特异性结合牛血清白蛋白核酸适配体的筛选方法
EP3670666A1 (en) 2018-12-20 2020-06-24 Freie Universität Berlin Method for optical imaging a target molecule in a sample, a protein-oligonuleotide conjugate for use in the method and a kit for carrying out the method
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
KR102513569B1 (ko) * 2019-03-19 2023-03-24 한국생명공학연구원 전사 시스템 기반 고감도 센서
CA3135206A1 (en) 2019-04-29 2020-11-05 Pierre Indermuhle Methods and systems for integrated on-chip single-molecule detection
CN110838134B (zh) * 2019-10-10 2020-09-29 北京海益同展信息科技有限公司 目标对象的统计方法、装置、计算机设备和存储介质
CN110746286B (zh) * 2019-11-04 2023-04-07 北京维德维康生物技术有限公司 一种丁香酚半抗原、人工抗原及其制备方法与应用
EP4090764A1 (en) 2020-01-13 2022-11-23 Becton, Dickinson and Company Cell capture using du-containing oligonucleotides
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
EP4111168A1 (en) 2020-02-25 2023-01-04 Becton Dickinson and Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CN111830014B (zh) * 2020-08-04 2023-06-30 济南大学 一种基于聚苯胺吸附双链dna的传感器检测dna的方法
CA3196729A1 (en) * 2020-11-11 2022-05-19 Tural AKSEL Affinity reagents having enhanced binding and detection characteristics
DE102020131047B4 (de) * 2020-11-24 2024-08-22 Abberior Instruments Gmbh Verfahren und Vorrichtung zur Aufnahme nanoskopischer Bilder mehrfach gefärbter Proben
EP4263859A1 (en) 2020-12-15 2023-10-25 Becton, Dickinson and Company Single cell secretome analysis
KR102573998B1 (ko) 2020-12-30 2023-09-04 주식회사 스타노스 위양성 신호가 제거된 항원 검출방법 및 키트
US20220252514A1 (en) * 2021-02-10 2022-08-11 Star Voltaic, LLC Fluorescent solid-state materials for optical calibration and methods thereof
EP4092414B1 (en) * 2021-05-19 2025-01-29 Leica Microsystems CMS GmbH Connector, marker and method for analysing biological samples
CN118414544A (zh) 2021-12-21 2024-07-30 生物辐射实验室股份有限公司 通过测量局部荧光比率在高分辨率装置中进行多重检测
WO2024168093A2 (en) * 2023-02-08 2024-08-15 Toreador Therapeutics, Inc. Super-resolution microscopy (srm) multiplexing with cleavable moieties
GB202304755D0 (en) * 2023-03-30 2023-05-17 Univ Ramot Method of imaging biomolecules
CN116165187B (zh) * 2023-04-23 2023-08-29 深圳大学 一种基于基准标记物的漂移矫正与筛选方法及成像方法
CN116794290B (zh) * 2023-07-03 2025-04-08 上海市第十人民医院 一种基于可逆dna自组装的组织切片靶标蛋白多重分析方法
EP4488682A1 (en) * 2023-07-06 2025-01-08 Leica Microsystems CMS GmbH Method and system for analysing a biological sample
WO2026008821A1 (en) 2024-07-05 2026-01-08 Miltenyi Biotec B.V. & Co. KG Method for continuous single molecule localization of conventional fluorescent antibody probes

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004009848A1 (en) * 2002-07-24 2004-01-29 Xing-Xiang Li Microparticle based signal amplification for the detection of analytes
WO2012058638A2 (en) * 2010-10-29 2012-05-03 President And Fellows Of Harvard College Nucleic acid nanostructure barcode probes
WO2012071428A2 (en) * 2010-11-22 2012-05-31 Solulink, Inc. Methods and/or use of oligonucleotide conjugates for assays and detections

Family Cites Families (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5424413A (en) 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
US5681943A (en) 1993-04-12 1997-10-28 Northwestern University Method for covalently linking adjacent oligonucleotides
US7569341B2 (en) * 1994-01-31 2009-08-04 Trustees Of Boston University Nucleic acid directed immobilization arrays and methods of assembly
DE4421891C2 (de) 1994-06-23 1996-05-15 Helmut Prof Dr Med Feucht Verfahren zum Nachweis von komplementaktivierenden IgG-Antikörpern gegen HLA-DR-Antigene
US5491063A (en) 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
DE19709348C2 (de) 1996-05-29 1999-07-01 Schubert Walter Dr Md Automatisches Multi-Epitop-Ligand-Kartierungsverfahren
US6146828A (en) 1996-08-14 2000-11-14 Exact Laboratories, Inc. Methods for detecting differences in RNA expression levels and uses therefor
US6117631A (en) * 1996-10-29 2000-09-12 Polyprobe, Inc. Detection of antigens via oligonucleotide antibody conjugates
GB9622665D0 (en) * 1996-10-31 1997-01-08 Zeneca Ltd Methods
DE19717904A1 (de) 1997-04-23 1998-10-29 Diagnostikforschung Inst Säurelabile und enzymatisch spaltbare Farbstoffkonstrukte zur Diagnostik mit Nahinfrarotlicht und zur Therapie
US7101672B2 (en) 1998-05-05 2006-09-05 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
US5888778A (en) 1997-06-16 1999-03-30 Exact Laboratories, Inc. High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers
US20100081134A1 (en) 1997-07-21 2010-04-01 Mirkin Chad A Bio-barcode based detection of target analytes
US6083486A (en) 1998-05-14 2000-07-04 The General Hospital Corporation Intramolecularly-quenched near infrared fluorescent probes
US7399844B2 (en) * 1998-07-09 2008-07-15 Agilent Technologies, Inc. Method and reagents for analyzing the nucleotide sequence of nucleic acids
AU4976699A (en) 1998-07-10 2000-02-01 Chromagen, Inc. Novel fluorogenic substrates for hydrolytic enzymes
US6573043B1 (en) 1998-10-07 2003-06-03 Genentech, Inc. Tissue analysis and kits therefor
US6468785B1 (en) 1999-02-19 2002-10-22 New Mexico State University Technology Transfer Corporation Doped conducting polymers applications and methods
WO2000058507A1 (en) 1999-03-30 2000-10-05 Solexa Ltd. Polynucleotide sequencing
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
DE10014685B4 (de) 2000-03-24 2004-07-01 Schubert, Walter, Dr. Verfahren zur Identifizierung von zellspezifischen Zielstrukturen
US20020142310A1 (en) 2000-04-07 2002-10-03 Leif Andersson Variants of the human AMP-activated protein kinase gamma 3 subunit
US20020106648A1 (en) 2000-05-05 2002-08-08 Lizardi Paul M. Highly multiplexed reporter carrier systems
JP2003535063A (ja) * 2000-06-01 2003-11-25 ザ・ボード・オブ・リージェンツ・フォー・オクラホマ・ステート・ユニバーシティー 放射線医薬としてのナノ粒子のバイオコンジュゲート
US6511809B2 (en) * 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
US6451588B1 (en) 2000-06-30 2002-09-17 Pe Corporation (Ny) Multipartite high-affinity nucleic acid probes
US20030175852A1 (en) 2000-09-15 2003-09-18 Kalra Krishan L Ehancement of in situ hybridization
CA2423729A1 (en) 2000-10-06 2002-04-11 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
US20030044353A1 (en) 2001-01-05 2003-03-06 Ralph Weissleder Activatable imaging probes
US20050230255A1 (en) 2001-03-30 2005-10-20 Sumner Lloyd W Silver destaining method
US7219016B2 (en) 2001-04-20 2007-05-15 Yale University Systems and methods for automated analysis of cells and tissues
US20020173053A1 (en) 2001-04-27 2002-11-21 Bassam Damaj Multiple simultaneous antigen detection by immunohistochemistry
US7473767B2 (en) * 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
AU2002319613A1 (en) 2001-07-19 2003-03-03 Signet Laboratories, Inc. Human tissue specific drug screening procedure
EP1430150B1 (en) 2001-09-24 2015-08-19 One Lambda, Inc. Diagnostic probe detection system
US8323903B2 (en) 2001-10-12 2012-12-04 Life Technologies Corporation Antibody complexes and methods for immunolabeling
WO2003061711A2 (en) 2002-01-16 2003-07-31 Visen Medical, Inc. Chromophore probes for optical imaging
AU2003291249A1 (en) 2002-12-18 2004-07-29 Aclara Biosciences, Inc. Multiplexed immunohistochemical assays using molecular tags
AU2004232976B2 (en) 2003-04-18 2011-02-10 Becton, Dickinson And Company Immuno-amplification
US20040241776A1 (en) 2003-05-22 2004-12-02 Agdia, Inc. Multiplex enzyme-linked immunosorbent assay for detecting multiple analytes
US7279280B2 (en) 2003-09-25 2007-10-09 Mgp Biotech, Inc. Apparatus and method for detecting genetic mutations and single nucleotide polymorphisms
US20050095595A1 (en) 2003-10-29 2005-05-05 Pittaro Richard J. Methods and systems for improving of polymer analysis
CA2545252A1 (en) * 2003-11-06 2005-05-26 University Of Nevada, Reno Improved methods for detecting and measuring specific nucleic acid sequences
US20050287578A1 (en) 2004-06-28 2005-12-29 Exagen Diagnostics, Inc. Methods for RNA fluorescence in situ hybridization
US8153363B2 (en) 2005-01-13 2012-04-10 Progenika Biopharma S.A. Methods and products for in vitro genotyping
US7727721B2 (en) * 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
US20060204999A1 (en) 2005-03-14 2006-09-14 Stephen Macevicz Detecting molecular complexes
US7795009B2 (en) 2005-06-15 2010-09-14 Saint Louis University Three-component biosensors for detecting macromolecules and other analytes
US8956857B2 (en) 2005-06-06 2015-02-17 Mediomics, Llc Three-component biosensors for detecting macromolecules and other analytes
US20070048759A1 (en) 2005-06-10 2007-03-01 Dan Luo Detection of target molecules with labeled nucleic acid detection molecules
US7842793B2 (en) 2005-06-14 2010-11-30 The California Institute Of Technology Methods of making nucleic acid nanostructures
US7494776B2 (en) 2005-07-07 2009-02-24 Beckman Coulter, Inc. Labeled complementary oligonucleotides to detect oligonucleotide-linked ligands
US20070259345A1 (en) 2006-05-03 2007-11-08 Agilent Technologies, Inc. Target determination using compound probes
US20080004185A1 (en) * 2006-06-12 2008-01-03 Antara Biosciences Inc. Three dimensional array
US7838302B2 (en) * 2006-08-07 2010-11-23 President And Fellows Of Harvard College Sub-diffraction limit image resolution and other imaging techniques
US7741045B2 (en) 2006-11-16 2010-06-22 General Electric Company Sequential analysis of biological samples
US20080242560A1 (en) 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
WO2008144562A1 (en) 2007-05-16 2008-11-27 California Institute Of Technology A versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
US8481714B2 (en) 2007-11-19 2013-07-09 Japan Advanced Institute Of Science And Technology Light-responsive artificial nucleotide having photo-crosslinking ability
US8309306B2 (en) 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
EP2350657A4 (en) 2008-11-21 2013-06-12 Univ Saint Louis BIOSENSOR FOR DETECTING MULTIPLE EPITOPES ON ONE TARGET
DE102008062372B3 (de) * 2008-12-17 2010-06-17 Medizinische Hochschule Hannover Nachweiskonjugat und Verfahren zur Analyse
US8785615B2 (en) * 2009-03-25 2014-07-22 Georgia Tech Research Corporation Single molecule sensitive probes for detecting RNA
US9677125B2 (en) 2009-10-21 2017-06-13 General Electric Company Detection of plurality of targets in biological samples
WO2011056864A1 (en) 2009-11-05 2011-05-12 Anaptysbio, Inc. Methods of generating improved antigen-binding agents using chain shuffling and optionally somatic hypermutation
US8586301B2 (en) 2010-06-30 2013-11-19 Stratos Genomics, Inc. Multiplexed identification of nucleic acid sequences
US20120004132A1 (en) 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
WO2012051386A2 (en) 2010-10-14 2012-04-19 Meso Scale Technologies, Llc Reagent storage in an assay device
US20120178081A1 (en) 2010-12-31 2012-07-12 Affymetrix. Inc. Methods of Labeling Cells, Labeled Cells, and uses Thereof
US8946389B2 (en) * 2011-04-25 2015-02-03 University Of Washington Compositions and methods for multiplex biomarker profiling
JP5989109B2 (ja) * 2011-07-13 2016-09-07 ライカ バイオシステムズ イメージング インコーポレイテッド 蛍光顕微鏡システムの標準化
WO2013016356A1 (en) * 2011-07-25 2013-01-31 Mad City Labs, Inc. Active-feedback positional drift correction in a microscope image using a fiduciary element held on a nanopositioning stage
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
WO2014028538A2 (en) 2012-08-13 2014-02-20 William Marsh Rice University Multiplexed in situ molecular analyses and programmable molecular probes for regulated signal amplification
US10584381B2 (en) * 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9975916B2 (en) 2012-11-06 2018-05-22 President And Fellows Of Harvard College Compositions and methods relating to complex nucleic acid nanostructures
WO2014076214A1 (en) 2012-11-14 2014-05-22 Olink Ab Rca reporter probes and their use in detecting nucleic acid molecules
CN105378477B9 (zh) 2013-03-11 2021-08-06 梅索磅秤技术有限公司 用于进行多路测定法的改进方法
US8933261B2 (en) * 2013-03-14 2015-01-13 Andrei POLUKHTIN Benzophenone-based chromophoric crosslinkers and reagents for incorporation of biotin or other haptens into macromolecules
WO2014145620A2 (en) 2013-03-15 2014-09-18 The Broad Institute, Inc. Novel hybridization probes and uses thereof
AU2014296253A1 (en) 2013-07-30 2016-02-04 President And Fellows Of Harvard College Quantitative DNA-based imaging and super-resolution imaging
WO2015070037A2 (en) 2013-11-08 2015-05-14 Prognosys Biosciences, Inc. Polynucleotide conjugates and methods for analyte detection
CN106062211A (zh) 2013-12-15 2016-10-26 哈佛学院院长等 与光学超分辨率图案化相关的方法和组合物
EP3116889A4 (en) 2014-03-08 2017-08-02 President and Fellows of Harvard College Nucleic acid polyhedra from self-assembled vertex-containing fixed-angle nucleic acid structures
EP4245859A3 (en) 2014-03-11 2023-11-08 President and Fellows of Harvard College High-throughput and highly multiplexed imaging with programmable nucleic acid probes
US20160033411A1 (en) 2014-07-29 2016-02-04 President And Fellows Of Harvard College Methods and compositions relating to storm-based patterning
DK3218511T3 (da) 2014-11-11 2020-07-20 Illumina Cambridge Ltd Fremgangsmåder og arrays til frembringelse og sekventering af monoklonale klynger af nukleinsyre
US10006917B2 (en) 2014-12-15 2018-06-26 President And Fellows Of Harvard College Methods and compositions relating to super-resolution imaging and modification
US20180224461A1 (en) 2015-08-07 2018-08-09 President And Fellows Of Harvard College Super resolution imaging of protein-protein interactions
US20230176064A1 (en) 2018-08-28 2023-06-08 Jl Medilabs, Inc. Methods and kits for detecting target substances

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004009848A1 (en) * 2002-07-24 2004-01-29 Xing-Xiang Li Microparticle based signal amplification for the detection of analytes
WO2012058638A2 (en) * 2010-10-29 2012-05-03 President And Fellows Of Harvard College Nucleic acid nanostructure barcode probes
WO2012071428A2 (en) * 2010-11-22 2012-05-31 Solulink, Inc. Methods and/or use of oligonucleotide conjugates for assays and detections

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANDREA L等: "Fluorescent DNA Nanotags Featuring Covalently Attached Intercalating Dyes: Synethesis, Antibody Conjugation, and Intracellular Imaging", 《BIOCONJUGATE CHEMISTRY》 *
DANIEL J. NIEVES 等: "DNA-Based Super-Resolution Microscopy: DNA-PAINT", 《GENES》 *
RALF JUNGMANN 等: "Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT", 《NATURE METHODS》 *
RALF JUNGMANN 等: "Single-Molecule Kinetics and Super-Resolution Microscopy by Fluorescence Imaging of Transient Binding on DNA Origami", 《NANOLETTERS》 *

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US11788123B2 (en) 2017-05-26 2023-10-17 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
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