CN105475108A - Culture method of brachytheciaceae - Google Patents
Culture method of brachytheciaceae Download PDFInfo
- Publication number
- CN105475108A CN105475108A CN201610048490.1A CN201610048490A CN105475108A CN 105475108 A CN105475108 A CN 105475108A CN 201610048490 A CN201610048490 A CN 201610048490A CN 105475108 A CN105475108 A CN 105475108A
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- Prior art keywords
- nutrient solution
- blue
- brachytheciaceae
- green moss
- grams per
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
- A01G24/35—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds containing water-absorbing polymers
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Hydroponics (AREA)
- Cultivation Of Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a culture method of brachytheciaceae. The culture method comprises the following steps: picking up brachytheciaceae growing in the field; spreading the brachytheciaceae on a culture substrate, wherein the culture substrate is obtained by mixing vermiculite and pearlite according to the volume ratio of 1:1; putting the spread brachytheciaceae at a place where the sun cannot shine on the spread brachytheciaceae directly; carrying out culture at the room temperature while keeping the humidity of 70-80%; pouring a nutrient solution every other day, wherein the pouring quantity is 1 to 2 ml/cm<2>, the nutrient solution is a Knop nutrient solution of which the concentration is 1/8, and the pH value of the nutrient solution is 6.0 to 6.5; pouring water every third day, wherein the pouring quantity is 2 to 4 ml/cm<2>. According to the culture method, the brachytheciaceae growing in the field is transplanted to the most proper substrate, the most proper nutrient solution is sprayed on the transplanted brachytheciaceae periodically, and the transplanted brachytheciaceae propagates indoor, so that the transplanted brachytheciaceae grows fast; no special requirements on experiment equipment are required; the operation is simple; the cost is low; popularization and application of brachytheciaceae are facilitated.
Description
Technical field
The present invention relates to the cultural method of a kind of blue or green moss, belong to the cultivation of bryophyte, propagation technique field.
Background technology
At present, bryophyte is widely used in Landscape, especially liver moss microlandschaft increased popularity, larger to the demand of bryophyte.Blue or green moss (formal name used at school: Brachytheciumalbicans) belongs to Pleurocarpous mosses, and growth in flakes of crawling, just as green lawn, can beautify the environment and implicit water source.It is numerous that prior art carries out expansion mainly through tissue cultures, requires higher to the experimental technique of experimental facilities, experimental situation and operator, usually can because of experimenter's operation not even microbiological contamination, and all that has been achieved is spoiled, takes time and effort.
Summary of the invention
For above-mentioned prior art, the invention provides the cultural method of a kind of blue or green moss, the method does not have particular/special requirement to experimental facilities, simple to operate, and cost is low, is conducive to applying of blue or green moss.Present invention also offers the culture matrix that the most applicable blue or green moss is transplanted, the nutrient solution of the most applicable blue or green moss flourish.
The present invention is achieved by the following technical solutions:
A cultural method for blue or green moss, as follows: to be fetched by the blue or green moss of field grown, be laid on culture matrix, be placed in without sunlight direct irradiation place, cultivate under room temperature, keep humidity 70% ~ 80%, every other day water one time of nutrition liquid, the amount of watering is 1 ~ 2mL/cm
2, water a water every three days, the amount of watering is 2 ~ 4mL/cm
2.The amount of watering of nutrient solution and water all to be crawled occupied areal calculation according to blue or green moss.
Described culture matrix is formed according to the proportions of volume ratio 1: 1 by vermiculite and perlite, compound method is: get vermiculite (specification: particle diameter 2 ~ 4mm) and perlite (specification: particle diameter 2.5 ~ 7mm), according to the ratio mixing mixing of volume ratio 1: 1, to obtain final product.
Described nutrient solution is the Knop nutrient solution of 1/8 concentration, pH value 6.0 ~ 6.5; Described Knop nutrient solution (for nutrient solution commonly known in the art) is made up of nitrate of lime, magnesium sulfate, potassium nitrate, ferrous sulfate, potassium dihydrogen phosphate and water, wherein, each concentration of component is: nitrate of lime 0.8 grams per liter, magnesium sulfate 0.2 grams per liter, potassium nitrate 0.2 grams per liter, potassium dihydrogen phosphate 0.2 grams per liter, ferrous sulfate trace (< 0.5mmol/L).
The cultural method of blue or green moss of the present invention, transplanted to the suitableeest matrix and periodically sprinkle Optimum nutrition liquid by blue or green for field moss, breed in indoor, growth is fast, and do not have particular/special requirement to experimental facilities, simple to operate, cost is low.
Accompanying drawing explanation
Fig. 1: under different culture media matter, blue or green moss growth rate compares.
Fig. 2: under Different Nutrition liquid, blue or green moss biomass compares.
Under Fig. 3: different K nop nutrient solution concentration, blue or green moss growth rate compares.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Instrument, reagent, material etc. involved in following embodiment, unless otherwise noted, be existing conventional instrument, reagent, material etc. in prior art, obtain by regular commercial sources.Experimental technique involved in following embodiment, detection method etc., unless otherwise noted, are existing normal experiment method in prior art, detection method etc.
Test the screening of the best cultural method of blue or green moss
1. method
The preparation of 1.1 culture fluids
The nutrient solution 3L of 1X concentration is first made according to the formula (being medium commonly known in the art) of Beneck, MS and Knop nutrient solution.Then get the Knop culture fluid 500mL of 1X concentration, with distilled water diluting, be mixed with to 1L, now nutrient solution concentration is 1/2X, in like manner can dilute and obtain the nutrient solution 1L that concentration is 1/8X.The pH value of the nutrient solution of 3 kinds of nutrient solutions and variable concentrations thereof adjusts to 6.0 ~ 6.5.
The preparation of 1.2 culture matrixes
The composition of culture matrix comprises vermiculite (particle diameter 2 ~ 4mm) and perlite (particle diameter 2.5 ~ 7mm), and the volume ratio respectively according to 1: 1,1: 2 and 1: 3 during preparation carries out.After preparing, be dispensed in 3 flowerpots, water and make it keep moistening.
1.3 transplant and breeding
Blue or green moss is transplanted and breeding is divided into three groups according to the culture fluid of culture matrix, sprinkling and variable concentrations thereof.Group 1 comprises 3 kinds of different substrates, and namely vermiculite and perlite ratio are respectively 1: 1,1: 2 and 1: 3.All spray 1XKnop nutrient solution.Group 2 sprays 3 kinds of different nutrient solutions respectively, i.e. Beneck, MS and Knop.Concentration is 1X, and matrix is vermiculite: perlite=1: 1.Group 3 sprays the Knop nutrient solution of 3 kinds of variable concentrations respectively, i.e. 1X, 1/2X and 1/8X.Matrix is vermiculite: perlite=1: 1.Blue or green moss slices are laid in different rectangle flowerpots (including culture matrix), cover pressure gently, make it and matrix compact siro spinning technology, the indoor again they being put into together the external conditions such as temperature (20 ~ 25 DEG C), humidity (70 ~ 80%), illumination (1500 ~ 2000lux, 10 ~ 12h/d) identical are bred.In bimestrial experiment, watered 1 water (2 ~ 4mL/cm every 2 days
2), 1 nutrient solution (1 ~ 2mL/cm was watered every 1 day
2).
The mensuration of 1.4 biomasss
Two months periods of cultivating, every 20 days, respectively from the blue or green moss of group 1 (different culture media matter), group 2 (spraying Different Nutrition liquid) and group 3 (spraying variable concentrations Knop nutrient solution), shear 3 × 3cm sample prescription, put into 105 DEG C of baking ovens 30 minutes, dry.3 repetitions are set.Take out each sample prescription, shake off residual matrix and weigh on electronic balance respectively.Calculate the dry weight mean value (g/cm of the blue or green moss of unit are respectively
2).
2. result
Under 2.1 different culture media matter, blue or green moss biomass compares
Result is as shown in table 1, Fig. 1.
Blue or green moss biomass (unit: g/cm under table 1 different culture media matter
2)
From table 1 and Fig. 1, at temperature, illumination, humidity, culture fluid (Knop nutrient solution, concentration is 1 ×) etc. extraneous condition of culture identical when, it is blue or green moss growth rate the fastest (0.0033) in the culture matrix of 1: 1 (i.e. pH=6.35) at vermiculite and perlite ratio, ratio is took second place (0.0030) 1: 2 (namely during pH=6.09), and ratio is 1: 3 (namely during pH=5.8) the slowest (0.0024).
Under 2.2 Different Nutrition liquid, blue or green moss biomass compares
Result is as shown in table 2, Fig. 2.
Blue or green moss biomass (unit: g/cm under table 2 Different Nutrition liquid
2)
From table 2 and Fig. 2,1) etc. at temperature, illumination, humidity, culture matrix (vermiculite: perlite=1: extraneous condition of culture is identical, spray under being all Beneck, MS, Knop nutrient solution of 1X concentration, blue or green moss growth rate value is respectively 0.0074,0.0053,0.0086, shows that the Knop nutrient solution of 1X concentration is more conducive to the growth of blue or green moss.
Under 2.3 different K nop nutrient solution concentrations, blue or green moss biomass compares
Result is as shown in table 3, Fig. 3.
Blue or green moss biomass (unit: g/cm under table 3 different K nop nutrient solution concentration
2)
From table 3 and Fig. 3,1) etc. at temperature, illumination, humidity, culture matrix (vermiculite: perlite=1: extraneous condition of culture is identical, be under the Solution culture method of 1/8X, 1/2X, 1X in concentration, blue or green moss growth rate value is respectively 0.0123,0.0092,0.0066, shows that the nutrient solution of 1/8X concentration is more conducive to the growth of blue or green moss.
3. conclusion
Experimental result shows: when temperature, illumination, the extraneous condition of culture of humidity are identical, and be prepare culture matrix at 1: 1 with vermiculite and perlite ratio, spray concentration is the Knop nutrient solution of 1/8X, and blue or green moss grows the fastest.
Claims (8)
1. a cultural method for blue or green moss, is characterized in that: fetched by the blue or green moss of field grown, be laid on culture matrix, be placed in without sunlight direct irradiation place, cultivate under room temperature, keep humidity 70% ~ 80%, every other day water one time of nutrition liquid, the amount of watering is 1 ~ 2mL/cm
2, water a water every three days, the amount of watering is 2 ~ 4mL/cm
2; Described culture matrix is formed according to the proportions of volume ratio 1: 1 by vermiculite and perlite; Described nutrient solution is the Knop nutrient solution of 1/8 concentration, pH value 6.0 ~ 6.5.
2. the cultural method of blue or green moss according to claim 1, is characterized in that: the specification of described vermiculite is: particle diameter 2 ~ 4mm; Described perlitic specification is: particle diameter 2.5 ~ 7mm.
3. the cultural method of blue or green moss according to claim 1, it is characterized in that: described Knop nutrient solution is made up of nitrate of lime, magnesium sulfate, potassium nitrate, ferrous sulfate, potassium dihydrogen phosphate and water, wherein, each concentration of component is: nitrate of lime 0.8 grams per liter, magnesium sulfate 0.2 grams per liter, potassium nitrate 0.2 grams per liter, potassium dihydrogen phosphate 0.2 grams per liter, ferrous sulfate trace.
4. for cultivating a culture matrix for blue or green moss, it is characterized in that: formed according to the proportions of volume ratio 1: 1 by vermiculite and perlite.
5. culture matrix according to claim 4 is cultivating the application in blue or green moss.
6. for cultivating a nutrient solution for blue or green moss, it is characterized in that: the Knop nutrient solution being 1/8 concentration, pH value 6.0 ~ 6.5.
7. nutrient solution according to claim 6, it is characterized in that: described Knop nutrient solution is made up of nitrate of lime, magnesium sulfate, potassium nitrate, ferrous sulfate, potassium dihydrogen phosphate and water, wherein, each concentration of component is: nitrate of lime 0.8 grams per liter, magnesium sulfate 0.2 grams per liter, potassium nitrate 0.2 grams per liter, potassium dihydrogen phosphate 0.2 grams per liter, ferrous sulfate trace.
8. the application in blue or green moss cultivated by the nutrient solution described in claim 6 or 7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610048490.1A CN105475108A (en) | 2016-01-26 | 2016-01-26 | Culture method of brachytheciaceae |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106386483A (en) * | 2016-08-30 | 2017-02-15 | 中国科学院昆明植物研究所 | Rapid propagation method for Brachythecium kuroishicum |
| CN108450315A (en) * | 2018-03-07 | 2018-08-28 | 深圳市铁汉生态环境股份有限公司 | A kind of nutrient solution and rapid propagation method of bryophyte |
| CN109349078A (en) * | 2018-10-15 | 2019-02-19 | 宁德市农业科学研究所 | A kind of bog moss indoor cultivation method for Ganoderma of clean and environmental protection |
| CN115299329A (en) * | 2022-08-04 | 2022-11-08 | 内蒙古农业大学 | Method for culturing odontopathy and application thereof |
| CN115304419A (en) * | 2022-08-04 | 2022-11-08 | 内蒙古农业大学 | Preparation method and application of moss cortex |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1805682A (en) * | 2004-02-10 | 2006-07-19 | 株式会社Hero | Method of producing young moss seedlings, method of producing moss mat and young moss seedlings |
| CN103069989A (en) * | 2013-01-22 | 2013-05-01 | 山西大学 | Method for producing esthetic bryophyte bottle |
| CN104350932A (en) * | 2014-11-27 | 2015-02-18 | 西北农林科技大学 | Rapid cultivating method of sand moss crust |
-
2016
- 2016-01-26 CN CN201610048490.1A patent/CN105475108A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1805682A (en) * | 2004-02-10 | 2006-07-19 | 株式会社Hero | Method of producing young moss seedlings, method of producing moss mat and young moss seedlings |
| CN103069989A (en) * | 2013-01-22 | 2013-05-01 | 山西大学 | Method for producing esthetic bryophyte bottle |
| CN104350932A (en) * | 2014-11-27 | 2015-02-18 | 西北农林科技大学 | Rapid cultivating method of sand moss crust |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106386483A (en) * | 2016-08-30 | 2017-02-15 | 中国科学院昆明植物研究所 | Rapid propagation method for Brachythecium kuroishicum |
| CN106386483B (en) * | 2016-08-30 | 2018-06-22 | 中国科学院昆明植物研究所 | A kind of rapid propagation method for the leaf blueness moss that wrinkles |
| CN108450315A (en) * | 2018-03-07 | 2018-08-28 | 深圳市铁汉生态环境股份有限公司 | A kind of nutrient solution and rapid propagation method of bryophyte |
| CN109349078A (en) * | 2018-10-15 | 2019-02-19 | 宁德市农业科学研究所 | A kind of bog moss indoor cultivation method for Ganoderma of clean and environmental protection |
| CN109349078B (en) * | 2018-10-15 | 2020-11-13 | 宁德市农业科学研究所 | Clean and environment-friendly indoor cultivation method for sphagnum |
| CN115299329A (en) * | 2022-08-04 | 2022-11-08 | 内蒙古农业大学 | Method for culturing odontopathy and application thereof |
| CN115304419A (en) * | 2022-08-04 | 2022-11-08 | 内蒙古农业大学 | Preparation method and application of moss cortex |
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Application publication date: 20160413 |
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