CN105434472A - Method for extracting sheep placenta extract primary liquid from sheep placentas - Google Patents
Method for extracting sheep placenta extract primary liquid from sheep placentas Download PDFInfo
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- 210000002826 placenta Anatomy 0.000 title claims abstract description 93
- 241001494479 Pecora Species 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 29
- 239000007788 liquid Substances 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 49
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims abstract description 16
- 230000001954 sterilising effect Effects 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 7
- 238000004140 cleaning Methods 0.000 claims abstract description 6
- 238000001816 cooling Methods 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 235000013372 meat Nutrition 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 6
- 238000003828 vacuum filtration Methods 0.000 claims abstract description 6
- 241000283898 Ovis Species 0.000 claims description 69
- 230000003169 placental effect Effects 0.000 claims description 19
- 239000011550 stock solution Substances 0.000 claims description 15
- 239000012530 fluid Substances 0.000 claims description 13
- DKGAVHZHDRPRBM-UHFFFAOYSA-N Tert-Butanol Chemical compound CC(C)(C)O DKGAVHZHDRPRBM-UHFFFAOYSA-N 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 11
- VFLDPWHFBUODDF-FCXRPNKRSA-N curcumin Chemical compound C1=C(O)C(OC)=CC(\C=C\C(=O)CC(=O)\C=C\C=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-FCXRPNKRSA-N 0.000 claims description 10
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 7
- 238000012545 processing Methods 0.000 claims description 7
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 235000012754 curcumin Nutrition 0.000 claims description 5
- 239000004148 curcumin Substances 0.000 claims description 5
- 229940109262 curcumin Drugs 0.000 claims description 5
- VFLDPWHFBUODDF-UHFFFAOYSA-N diferuloylmethane Natural products C1=C(O)C(OC)=CC(C=CC(=O)CC(=O)C=CC=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-UHFFFAOYSA-N 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 5
- 229940083466 soybean lecithin Drugs 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 229920001661 Chitosan Polymers 0.000 claims description 4
- 241000033870 Citrullus lanatus subsp. vulgaris Species 0.000 claims description 4
- 235000012840 Citrullus vulgaris Nutrition 0.000 claims description 4
- 241000628997 Flos Species 0.000 claims description 4
- 102000015636 Oligopeptides Human genes 0.000 claims description 4
- 108010038807 Oligopeptides Proteins 0.000 claims description 4
- 235000004338 Syringa vulgaris Nutrition 0.000 claims description 4
- 210000002318 cardia Anatomy 0.000 claims description 4
- 150000004676 glycans Chemical class 0.000 claims description 4
- KBPHJBAIARWVSC-RGZFRNHPSA-N lutein Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\[C@H]1C(C)=C[C@H](O)CC1(C)C KBPHJBAIARWVSC-RGZFRNHPSA-N 0.000 claims description 4
- 229920001282 polysaccharide Polymers 0.000 claims description 4
- 239000005017 polysaccharide Substances 0.000 claims description 4
- 239000001397 quillaja saponaria molina bark Substances 0.000 claims description 4
- 150000003839 salts Chemical class 0.000 claims description 4
- 229930182490 saponin Natural products 0.000 claims description 4
- 150000007949 saponins Chemical class 0.000 claims description 4
- 210000000582 semen Anatomy 0.000 claims description 4
- 244000297179 Syringa vulgaris Species 0.000 claims 1
- 238000000605 extraction Methods 0.000 abstract description 4
- 238000010438 heat treatment Methods 0.000 abstract 2
- 239000012535 impurity Substances 0.000 abstract 1
- 239000008213 purified water Substances 0.000 abstract 1
- 241001104043 Syringa Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 230000036449 good health Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002607 hemopoietic effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 239000007901 soft capsule Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
- A61K8/982—Reproductive organs; Embryos, Eggs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Developmental Biology & Embryology (AREA)
- Reproductive Health (AREA)
- Epidemiology (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Dermatology (AREA)
- Pregnancy & Childbirth (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Birds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Cosmetics (AREA)
Abstract
The invention provides a method for extracting sheep placenta extract primary liquid from sheep placentas. The method comprises the following steps: (1) sorting fresh sheep placentas, then removing impurities, putting the sheep placentas in cleaning liquid to be cleaned, then fishing out the sheep placentas, draining off surface moisture and putting the sheep placentas in a fresh-keeping storehouse to be preserved; (2) taking out the sheep placentas in the fresh-keeping storehouse and putting the sheep placentas in warm water to be soaked for 10 minutes; (3) fishing out the sheep placentas and putting the sheep placentas in a meat grinder to be ground, thus obtaining ground sheep placentas; (4) refrigerating the ground sheep placentas in an environment at 4-6 DEG C for 3-8 hours and then carrying out discontinuous microwave treatment on the ground sheep placentas; (5) adding the ground sheep placentas subjected to microwave treatment to purified water, heating the ground sheep placentas to 45 DEG C, then adding xylanase, stirring the materials uniformly, maintaining the temperature at 45-60 DEG C and carrying out extraction for 2-4 hours, thus obtaining an extracting solution; (6) heating the extracting solution to 100 DEG C, maintaining the temperature for 30 minutes, carrying out vacuum filtration by utilizing a circulating water type multipurpose vacuum pump after cooling to obtain feed liquid after filtration and sterilizing and filling the feed liquid, thus obtaining the sheep placenta extract primary liquid.
Description
Technical field
The present invention relates to animal placenta extractive technique field, be specifically related to a kind of method extracting sheep placental extract stock solution from Placenta caprae seu ovis.
Background technology
Have good health and a long life from ancient times to the present, continuity life, perpetual youth always is the pursuit that the mankind never abandon, and along with the material progress development of human society, scientific and technological level improves constantly, and more excites people and enjoys life, and explores the desire of life miracle.The functional food of slow down aging, health invigorating, looks improving and the skin nourishing arises at the historic moment thereupon, and has considerable economic worth.
China is an animal husbandry big country, have a large amount of Placenta caprae seu ovis to produce every year, but most of Placenta caprae seu ovis is not able to sufficient exploitation, causes the great wasting of resources.The value of the bioactive substance extracted from a Placenta caprae seu ovis is far longer than the value of a sheep itself, as carried out comprehensive development and utilization to Placenta caprae seu ovis, not only can improve the income of local peasants and herdsmen, increase local finance tax revenue, also can improve China's commonwealth physical constitution, this will produce huge economic benefit and social benefit.
At present, domestic Placenta Hominis based article mainly contains: placental peptide injection, Placenta caprae seu ovis soft capsule, Placenta caprae seu ovis capsule, Placenta caprae seu ovis oral liquid, Placenta caprae seu ovis skin care liquid, Placenta caprae seu ovis moisturizing and nourishing frost etc.
Placenta caprae seu ovis is pharmaceutically acting on very extensively, and it has enhancing human body immunity function, regulates organism endocrine, maintains the magical effects such as body normal function, enhancing body endurance, hemopoietic function of improving, skin maintenance, slow down aging.But because processing technique and technological means fall behind, cause its active ingredient to lose very large.Current Placenta caprae seu ovis processing technique takes ground to be mostly Ordinary pulverization means, and make the active ingredient loss in Placenta caprae seu ovis serious, sheep placental extract extraction ratio reduces.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method extracting sheep placental extract stock solution from Placenta caprae seu ovis, and the method had both retained the natural active matter in Placenta Hominis, increased again its polypeptide and amino acid content.
Technical problem to be solved by this invention realizes by the following technical solutions:
From Placenta caprae seu ovis, extract a method for sheep placental extract stock solution, step is as follows:
(1) foreign material are removed after being sorted by fresh Placenta caprae seu ovis, put into cleanout fluid or pure water cleans, keep the temperature of cleanout fluid at 35-45 DEG C, first soak 35-40 minute, then pull out after cleaning up and drain surface moisture to put into temperature be 6-8 DEG C, humidity is at least preserve more than 4 hours in the fresh-keeping warehouse of 78-82%;
(2) Placenta caprae seu ovis in step (1) fresh-keeping warehouse is taken out, put into warm water and soak about 10 minutes, in every 500ml water temperature water, add corn peptide 5g, curcumin 5g, Chinese liquor 20ml and soybean lecithin 50g; Play sterilizing effect;
(3) Placenta caprae seu ovis in step (2) is pulled out, put into meat grinder, pulverize with the sieve plate of Φ 8-12mm, obtain the broken end of Placenta caprae seu ovis;
(4) by the cold preservation 3-8 hour under the environment of 4-6 DEG C of the broken end of Placenta caprae seu ovis in step (3), then uninterrupted microwave process is carried out to the broken end of Placenta caprae seu ovis, intermittent processing number of times is 4 ~ 10 times, and each break time is 40 seconds, and microwave treatment temperature is no more than 100 DEG C;
(5) the broken end of Placenta caprae seu ovis after microwave treatment in step (4) is joined in pure water, and be heated to 45 DEG C, then add xylanase and stir and maintain the temperature at 45 ~ 60 DEG C of lixiviates 2 ~ 4 hours, obtain extracting solution;
Wherein the adding proportion of the broken end of Placenta caprae seu ovis, xylanase and pure water is add the broken last 0.6-0.7kg of Placenta caprae seu ovis in every 1kg pure water, xylanase 0.03kg;
(6) extracting solution in step (5) is heated to 100 DEG C, keeps 30 minutes, utilize the multiplex vacuum pump of circulating water type to carry out vacuum filtration after cooling, obtain the feed liquid after sucking filtration, sterilization fill is carried out to feed liquid and can obtain sheep placental extract stock solution.
Above-mentioned cleanout fluid is formulated by the component of following weight: magnetic water 500kg, tert-butyl alcohol 50kg, chitosan 0.5kg, Saponin 0.3kg, Chinese liquor 2kg, Citrullus vulgaris polysaccharide 0.5kg, Cardia Salt 5kg, Semen Tritici aestivi oligopeptide 0.2kg, lutein ester 0.2kg, Flos Lonicerae extract 0.3kg, Caulis et Folium Brassicae capitatae 0.3kg, lilac water extract 0.3kg.
Preparation method disclosed by the invention, well solves the technical problem existing for sheep placental extract extraction in prior art.Production technology overcomes single Physical, chemical method or enzyme process and all removes the halfway technical barrier of precipitation, modern food new technique and traditional method are combined, microwave and enzyme extraction technology are applied in sheep placental extract production, effectively eliminate precipitation, ensure that the quality of product.
The invention has the beneficial effects as follows: process of manufacture of the present invention fully can extract the effective ingredient in Placenta caprae seu ovis, the active substance of Placenta caprae seu ovis is maximized the retention, is convenient to again human body overall absorption, technique is advanced, reasonable, quality controllable.The refining sheep placenta element of the sheep placenta adopting said method to obtain, had both retained the natural active matter in Placenta Hominis, had increased again its polypeptide and amino acid content.
Detailed description of the invention
The technological means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below in conjunction with specific embodiment, setting forth the present invention further.
Embodiment 1
From Placenta caprae seu ovis, extract a method for sheep placental extract stock solution, step is as follows:
(1) foreign material are removed after being sorted by fresh Placenta caprae seu ovis, put into cleanout fluid to clean, keep the temperature of cleanout fluid at 40 DEG C, first soak 40 minutes, then pull out after cleaning up and drain surface moisture to put into temperature be 7 DEG C, humidity is preserve more than 8 hours in the fresh-keeping warehouse of 80%;
(2) Placenta caprae seu ovis in step (1) fresh-keeping warehouse is taken out, put into warm water and soak about 10 minutes, in every 500ml water temperature water, add corn peptide 5g, curcumin 5g, Chinese liquor 20ml and soybean lecithin 50g; Play sterilizing effect;
(3) Placenta caprae seu ovis in step (2) is pulled out, put into meat grinder, pulverize with the sieve plate of Φ 10mm, obtain the broken end of Placenta caprae seu ovis;
(4) by the cold preservation 5 hours under the environment of 5 DEG C of the broken end of Placenta caprae seu ovis in step (3), then carry out uninterrupted microwave process to the broken end of Placenta caprae seu ovis, intermittent processing number of times is 8 times, and each break time is 40 seconds, and microwave treatment temperature is no more than 100 DEG C;
(5) the broken end of Placenta caprae seu ovis after microwave treatment in step (4) is joined in pure water, and be heated to 45 DEG C, then add xylanase and stir and maintain the temperature at 55 DEG C of lixiviates 3 hours, obtain extracting solution;
Wherein the adding proportion of the broken end of Placenta caprae seu ovis, xylanase and pure water is add the broken last 0.6-0.7kg of Placenta caprae seu ovis in every 1kg pure water, xylanase 0.03kg;
(6) extracting solution in step (5) is heated to 100 DEG C, keeps 30 minutes, utilize the multiplex vacuum pump of circulating water type to carry out vacuum filtration after cooling, obtain the feed liquid after sucking filtration, sterilization fill is carried out to feed liquid and can obtain sheep placental extract stock solution.
Above-mentioned cleanout fluid is formulated by the component of following weight: magnetic water 500kg, tert-butyl alcohol 50kg, chitosan 0.5kg, Saponin 0.3kg, Chinese liquor 2kg, Citrullus vulgaris polysaccharide 0.5kg, Cardia Salt 5kg, Semen Tritici aestivi oligopeptide 0.2kg, lutein ester 0.2kg, Flos Lonicerae extract 0.3kg, Caulis et Folium Brassicae capitatae 0.3kg, lilac water extract 0.3kg.
Embodiment 2
From Placenta caprae seu ovis, extract a method for sheep placental extract stock solution, step is as follows:
(1) foreign material are removed after being sorted by fresh Placenta caprae seu ovis, put into cleanout fluid to clean, keep the temperature of cleanout fluid at 45 DEG C, first soak 35 minutes, then pull out after cleaning up and drain surface moisture to put into temperature be 8 DEG C, humidity is preserve more than 6 hours in the fresh-keeping warehouse of 78%;
(2) Placenta caprae seu ovis in step (1) fresh-keeping warehouse is taken out, put into warm water and soak about 10 minutes, in every 500ml water temperature water, add corn peptide 5g, curcumin 5g, Chinese liquor 20ml and soybean lecithin 50g; Play sterilizing effect;
(3) Placenta caprae seu ovis in step (2) is pulled out, put into meat grinder, pulverize with the sieve plate of Φ 12mm, obtain the broken end of Placenta caprae seu ovis;
(4) by the cold preservation 8 hours under the environment of 4 DEG C of the broken end of Placenta caprae seu ovis in step (3), then carry out uninterrupted microwave process to the broken end of Placenta caprae seu ovis, intermittent processing number of times is 4 times, and each break time is 40 seconds, and microwave treatment temperature is no more than 100 DEG C;
(5) the broken end of Placenta caprae seu ovis after microwave treatment in step (4) is joined in pure water, and be heated to 45 DEG C, then add xylanase and stir and maintain the temperature at 60 DEG C of lixiviates 2 hours, obtain extracting solution;
Wherein the adding proportion of the broken end of Placenta caprae seu ovis, xylanase and pure water is add the broken last 0.6-0.7kg of Placenta caprae seu ovis in every 1kg pure water, xylanase 0.03kg;
(6) extracting solution in step (5) is heated to 100 DEG C, keeps 30 minutes, utilize the multiplex vacuum pump of circulating water type to carry out vacuum filtration after cooling, obtain the feed liquid after sucking filtration, sterilization fill is carried out to feed liquid and can obtain sheep placental extract stock solution.
Above-mentioned cleanout fluid is formulated by the component of following weight: magnetic water 500kg, tert-butyl alcohol 50kg, chitosan 0.5kg, Saponin 0.3kg, Chinese liquor 2kg, Citrullus vulgaris polysaccharide 0.5kg, Cardia Salt 5kg, Semen Tritici aestivi oligopeptide 0.2kg, lutein ester 0.2kg, Flos Lonicerae extract 0.3kg, Caulis et Folium Brassicae capitatae 0.3kg, lilac water extract 0.3kg.
Embodiment 3
From Placenta caprae seu ovis, extract a method for sheep placental extract stock solution, step is as follows:
(1) foreign material are removed after being sorted by fresh Placenta caprae seu ovis, put into pure water to clean, keep the temperature of cleanout fluid at 45 DEG C, first soak 40 minutes, then pull out after cleaning up and drain surface moisture to put into temperature be 6 DEG C, humidity is at least preserve more than 4 hours in the fresh-keeping warehouse of 82%;
(2) Placenta caprae seu ovis in step (1) fresh-keeping warehouse is taken out, put into warm water and soak about 10 minutes, in every 500ml water temperature water, add corn peptide 5g, curcumin 5g, Chinese liquor 20ml and soybean lecithin 50g; Play sterilizing effect;
(3) Placenta caprae seu ovis in step (2) is pulled out, put into meat grinder, pulverize with the sieve plate of Φ 8mm, obtain the broken end of Placenta caprae seu ovis;
(4) by the cold preservation 4 hours under the environment of 6 DEG C of the broken end of Placenta caprae seu ovis in step (3), then uninterrupted microwave process is carried out to the broken end of Placenta caprae seu ovis, intermittent processing number of times is 10 times, and each break time is 40 seconds, and microwave treatment temperature is no more than 100 DEG C;
(5) the broken end of Placenta caprae seu ovis after microwave treatment in step (4) is joined in pure water, and be heated to 45 DEG C, then add xylanase and stir and maintain the temperature at 60 DEG C of lixiviates 4 hours, obtain extracting solution;
Wherein the adding proportion of the broken end of Placenta caprae seu ovis, xylanase and pure water is add the broken last 0.6-0.7kg of Placenta caprae seu ovis in every 1kg pure water, xylanase 0.03kg;
(6) extracting solution in step (5) is heated to 100 DEG C, keeps 30 minutes, utilize the multiplex vacuum pump of circulating water type to carry out vacuum filtration after cooling, obtain the feed liquid after sucking filtration, sterilization fill is carried out to feed liquid and can obtain sheep placental extract stock solution.
More than show and describe ultimate principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and description just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (3)
1. from Placenta caprae seu ovis, extract a method for sheep placental extract stock solution, it is characterized in that, step is as follows:
(1) foreign material are removed after being sorted by fresh Placenta caprae seu ovis, put into cleanout fluid or pure water cleans, keep the temperature of cleanout fluid at 35-45 DEG C, first soak 35-40 minute, then pull out after cleaning up and drain surface moisture to put into temperature be 6-8 DEG C, humidity is at least preserve more than 4 hours in the fresh-keeping warehouse of 78-82%;
(2) Placenta caprae seu ovis in step (1) fresh-keeping warehouse is taken out, put into warm water and soak 10 minutes, in every 500ml water temperature water, add corn peptide 5g, curcumin 5g, Chinese liquor 20ml and soybean lecithin 50g;
(3) Placenta caprae seu ovis in step (2) is pulled out, put into meat grinder, pulverize with the sieve plate of Φ 8-12mm, obtain the broken end of Placenta caprae seu ovis;
(4) by the cold preservation 3-8 hour under the environment of 4-6 DEG C of the broken end of Placenta caprae seu ovis in step (3), then uninterrupted microwave process is carried out to the broken end of Placenta caprae seu ovis, intermittent processing number of times is 4 ~ 10 times, and each break time is 40 seconds, and microwave treatment temperature is no more than 100 DEG C;
(5) the broken end of Placenta caprae seu ovis after microwave treatment in step (4) is joined in pure water, and be heated to 45 DEG C, then add xylanase and stir and maintain the temperature at 45 ~ 60 DEG C of lixiviates 2 ~ 4 hours, obtain extracting solution;
(6) extracting solution in step (5) is heated to 100 DEG C, keeps 30 minutes, utilize the multiplex vacuum pump of circulating water type to carry out vacuum filtration after cooling, obtain the feed liquid after sucking filtration, sterilization fill is carried out to feed liquid and can obtain sheep placental extract stock solution.
2. a kind of method extracting sheep placental extract stock solution from Placenta caprae seu ovis according to claim 1, it is characterized in that, the adding proportion of the broken end of Placenta caprae seu ovis in above-mentioned steps (5), xylanase and pure water is add the broken last 0.6-0.7kg of Placenta caprae seu ovis in every 1kg pure water, xylanase 0.03kg.
3. a kind of method extracting sheep placental extract stock solution from Placenta caprae seu ovis according to claim 1, it is characterized in that, above-mentioned cleanout fluid is formulated by the component of following weight: magnetic water 500kg, tert-butyl alcohol 50kg, chitosan 0.5kg, Saponin 0.3kg, Chinese liquor 2kg, Citrullus vulgaris polysaccharide 0.5kg, Cardia Salt 5kg, Semen Tritici aestivi oligopeptide 0.2kg, lutein ester 0.2kg, Flos Lonicerae extract 0.3kg, Caulis et Folium Brassicae capitatae 0.3kg, lilac water extract 0.3kg.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510809086.7A CN105434472A (en) | 2015-11-19 | 2015-11-19 | Method for extracting sheep placenta extract primary liquid from sheep placentas |
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| CN201510809086.7A CN105434472A (en) | 2015-11-19 | 2015-11-19 | Method for extracting sheep placenta extract primary liquid from sheep placentas |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105851732A (en) * | 2016-04-07 | 2016-08-17 | 金寨金栗源生物技术有限公司 | Chinese chestnut protein peptide liquid enriched drink and preparation method thereof |
| CN107412136A (en) * | 2017-09-04 | 2017-12-01 | 银川凤仪堂生物工程有限公司 | Goat Placenta enzymatic hydrolysis and fermentation concentrate and preparation technology for cosmetic material |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105831774A (en) * | 2016-04-07 | 2016-08-10 | 金寨金栗源生物技术有限公司 | A glossy ganoderma peptide preparing method |
| CN105852137A (en) * | 2016-04-07 | 2016-08-17 | 金寨金栗源生物技术有限公司 | Preparation method and product of Chinese chestnut micro-molecular peptide flour |
| CN105851732A (en) * | 2016-04-07 | 2016-08-17 | 金寨金栗源生物技术有限公司 | Chinese chestnut protein peptide liquid enriched drink and preparation method thereof |
| CN107412136A (en) * | 2017-09-04 | 2017-12-01 | 银川凤仪堂生物工程有限公司 | Goat Placenta enzymatic hydrolysis and fermentation concentrate and preparation technology for cosmetic material |
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