CN107412136A - Goat Placenta enzymatic hydrolysis and fermentation concentrate and preparation technology for cosmetic material - Google Patents
Goat Placenta enzymatic hydrolysis and fermentation concentrate and preparation technology for cosmetic material Download PDFInfo
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- CN107412136A CN107412136A CN201710784967.7A CN201710784967A CN107412136A CN 107412136 A CN107412136 A CN 107412136A CN 201710784967 A CN201710784967 A CN 201710784967A CN 107412136 A CN107412136 A CN 107412136A
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- Prior art keywords
- enzymatic hydrolysis
- cosmetic material
- fermentation concentrate
- goat placenta
- placenta
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
- A61K8/982—Reproductive organs; Embryos, Eggs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Dermatology (AREA)
- Reproductive Health (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Zoology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Developmental Biology & Embryology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Cosmetics (AREA)
Abstract
A kind of Goat Placenta enzymatic hydrolysis and fermentation concentrate for cosmetic material, including frozen dry powder of sheep placenta, trypsase, papain, aspergillus niger and water.The above-mentioned Goat Placenta enzymatic hydrolysis and fermentation concentrate for cosmetic material be by aspergillus niger, frozen dry powder of sheep placenta, trypsase, papain and purified water successively according to weight ratio be 1:50:80:20:1000 enzymatic hydrolysis and fermentations that feed intake are made.The present invention also provides a kind of preparation technology of the Goat Placenta enzymatic hydrolysis and fermentation concentrate for cosmetic material.Goat Placenta enzymatic hydrolysis and fermentation concentrate provided by the invention is used for the raw material of cosmetics, and the Goat Placenta is prepared into cosmetic material, its all materials mainly includes frozen dry powder of sheep placenta, trypsase, papain, aspergillus niger and purified water through digesting and fermenting.Present invention can ensure that security of the cosmetics produced using this product as raw material to skin.
Description
Technical field：
The present invention relates to cosmetic technical field, more particularly to a kind of Goat Placenta enzymatic hydrolysis and fermentation for cosmetic material are dense
Contracting liquid and preparation technology.
Background technology：
The Goat Placenta aboundresources that China can develop, but utilization rate is low, causes the serious waste of resource, is making up
In product field, although the sheep placenta in Goat Placenta has the effect of beautifying and anti-aging, sheep placenta is carried in the prior art
The effect for taking and utilizing is less desirable, and still Goat Placenta resource can not be used well.
The content of the invention：
In view of this, it is necessary to which a kind of Goat Placenta enzymatic hydrolysis and fermentation concentrate for cosmetic material is provided.
It there is a need to and a kind of preparation technology of above-mentioned Goat Placenta enzymatic hydrolysis and fermentation concentrate is provided.
A kind of Goat Placenta enzymatic hydrolysis and fermentation concentrate for cosmetic material, including frozen dry powder of sheep placenta, trypsase, wood
Melon protease, aspergillus niger and water.
The above-mentioned Goat Placenta enzymatic hydrolysis and fermentation concentrate for cosmetic material is by aspergillus niger, frozen dry powder of sheep placenta, pancreas egg
White enzyme, papain and purified water are successively 1 according to weight ratio:50:80:20:1000 enzymatic hydrolysis and fermentations that feed intake are made.
Water used in the above-mentioned Goat Placenta enzymatic hydrolysis and fermentation concentrate for cosmetic material is purified water, Goat Placenta used
The mesh number of freeze-dried powder is the mesh of 20 mesh~50.
A kind of preparation technology of Goat Placenta enzymatic hydrolysis and fermentation concentrate for cosmetic material, is comprised the following steps that：
Step 1, it is standby that Goat Placenta is prepared into freeze-dried powder；
Step 2, quantitative frozen dry powder of sheep placenta is dissolved with water, and centrifugal treating, collect supernatant, residue
It is standby；
Step 3, aspergillus niger culture medium is prepared, the residue in step 2 is added into water, is configured to aspergillus niger culture medium, so
High-temperature sterilization processing is carried out afterwards；
Step 4, the aspergillus niger culture medium in step 3 is recovered to 15 degrees Celsius~20 degrees Celsius, then to aspergillus niger
Trypsase and papain are added in culture medium, and is stirred, is then put into water-bath and digests, obtain enzymolysis liquid；
Step 5, by the enzymolysis liquid autoclaving in step 4, then make its temperature revert to 15 degrees Celsius to 25 it is Celsius
Degree, then adds aspergillus niger, stirs, and ferments in the environment of being 28 degrees Celsius in temperature, then samples once within every 24 hours；
Step 6, the zymotic fluid obtained in step 5 is subjected to centrifugal treating, then collects supernatant；
Step 7, the attenuating that the supernatant in step 6 is carried out to conductance handle, then rotate obtained solution
Evaporimeter is concentrated, and obtains the Goat Placenta enzymatic hydrolysis and fermentation concentrate for cosmetic material, the Goat Placenta enzymatic hydrolysis and fermentation concentrate
Available in the composition of raw materials of cosmetics.
In the above-mentioned preparation technology for the Goat Placenta enzymatic hydrolysis and fermentation concentrate of cosmetic material, water used is purifying
Water, the mesh number of frozen dry powder of sheep placenta are the mesh of 20 mesh~50.
, will be quantitative in step 2 in the above-mentioned preparation technology for the Goat Placenta enzymatic hydrolysis and fermentation concentrate of cosmetic material
Frozen dry powder of sheep placenta dissolved with water, and during centrifugal treating, once, the rotating speed of centrifugation is 2000- to centrifugal treating
3000r/min, centrifugation time 4-10min.
In step three in the above-mentioned preparation technology for the Goat Placenta enzymatic hydrolysis and fermentation concentrate of cosmetic material, aspergillus niger
Culture medium carries out high-temperature sterilization processing, and autoclaving is carried out 20 minutes in the environment of being 121 degrees Celsius in temperature.
In the step of above-mentioned preparation technology for the Goat Placenta enzymatic hydrolysis and fermentation concentrate of cosmetic material four, in water-bath
The temperature of middle enzymolysis is 30 degrees Celsius~58 degrees Celsius, and enzymolysis time is 1 hour~3 hours.
In the step of above-mentioned preparation technology for the Goat Placenta enzymatic hydrolysis and fermentation concentrate of cosmetic material five, by step 4
In enzymolysis liquid autoclaving, temperature control be 121 degrees Celsius, time control be 20 minutes, the aspergillus niger is with conidia powder
Form add.
In the above-mentioned preparation technology for the Goat Placenta enzymatic hydrolysis and fermentation concentrate of cosmetic material, by what is obtained in step 5
Zymotic fluid carries out centrifugal treating, and the rotating speed of centrifugation is 2000-3000r/min, centrifugation time 4-10min.
In the step of above-mentioned preparation technology for the Goat Placenta enzymatic hydrolysis and fermentation concentrate of cosmetic material seven, using D296
Type anion and D61 types resin cation reduce the pretreatment of conductance to the supernatant in step 6.
In the above-mentioned preparation technology for the Goat Placenta enzymatic hydrolysis and fermentation concentrate of cosmetic material, described aspergillus niger is true
Bacterium.
Goat Placenta enzymatic hydrolysis and fermentation concentrate provided by the invention is used for the raw material of cosmetics, and the Goat Placenta is through digesting and sending out
Ferment, cosmetic material is prepared into, its all materials mainly includes frozen dry powder of sheep placenta, trypsase, papain, aspergillus niger
And purified water.The present invention is digested to Goat Placenta using trypsase and papain and is used fermentation of Aspergillus niger again, is remained
When Goat Placenta functional component and its activity, avoiding active component caused by enzymolysis and fermentation process and be lost in, and preparing not
Add any organic reagent, it can be ensured that security of the cosmetics produced using this product as raw material to skin.It is rich in product
Sheep embryo extract, amino acid and oligopeptides, it has significant anti-oxidant, anti-aging, Bearberry Extract.
Embodiment：
The invention provides a kind of implementation of the preparation technology of the Goat Placenta enzymatic hydrolysis and fermentation concentrate for cosmetic material
Example：
Step 1, it is standby that Goat Placenta is prepared into freeze-dried powder；The mesh number of frozen dry powder of sheep placenta is the mesh of 20 mesh~50；
Step 2, quantitative frozen dry powder of sheep placenta is dissolved with water, and centrifugal treating, centrifugal treating once, centrifuge
Rotating speed be 2000-3000r/min, centrifugation time 4-10min, collect supernatant, residue is standby；
Step 3, aspergillus niger culture medium is prepared, the residue in step 2 is added into water, is configured to aspergillus niger culture medium, so
High-temperature sterilization processing is carried out afterwards；Aspergillus niger culture medium carries out high-temperature sterilization processing, is carried out in the environment of being 121 degrees Celsius in temperature
Autoclaving 20 minutes；
Step 4, the aspergillus niger culture medium in step 3 is recovered to 15 degrees Celsius~20 degrees Celsius, then to aspergillus niger
Trypsase and papain are added in culture medium, and is stirred, is then put into water-bath and digests, obtain enzymolysis liquid；In water
The temperature digested in bath is 30 degrees Celsius~58 degrees Celsius, and enzymolysis time is 1 hour~3 hours；
Step 5, by the enzymolysis liquid autoclaving in step 4, temperature control is 121 degrees Celsius, and time control is 20 points
Clock, its temperature is reverted to 15 degrees Celsius to 25 degrees Celsius, then add aspergillus niger, stir, taken the photograph in temperature for 28
Ferment in the environment of family name's degree, then sample once within every 24 hours；The aspergillus niger is added in the form of conidia powder, and aspergillus niger is
Fungi；
Step 6, the zymotic fluid obtained in step 5 is subjected to centrifugal treating, then collects supernatant；The rotating speed of centrifugation is
2000-3000r/min, centrifugation time 4-10min；
Step 7, the supernatant in step 6 is carried out reduction and led using D296 types anion and D61 types resin cation
The pretreatment of electric rate, then obtained solution is concentrated with Rotary Evaporators, obtain the Goat Placenta enzyme for cosmetic material
Hydrolysis and fermentation concentrate, the Goat Placenta enzymatic hydrolysis and fermentation concentrate can be used in the composition of raw materials of cosmetics.
Cosmetics prepared by the Goat Placenta enzymatic hydrolysis and fermentation concentrate obtained using the present invention have higher DPPH, amino acid
Content, protein content etc. are a little.
The present invention uses trypsase and papain enzymolysis Goat Placenta, not only successfully obtains the function of Goat Placenta
Property component, and polypeptide is rich in, accomplish to make full use of Goat Placenta resource.Aspergillus niger decomposable asymmetric choice net many kinds enzyme is used in fermentation,
Such as：Acid protease and citric acid etc.；Obtained bioactive small molecule peptide and amino acid content is high, and skin easily absorbs.This hair
The preparation technology of the Goat Placenta enzymatic hydrolysis and fermentation concentrate for cosmetic material of bright offer is that a kind of digested using double enzymes is sent out again
Ferment technique, it can obtain that there is excellent antioxidant, the product of wrinkle resistance.Using double enzymes enzymolysis, zymotechnique obtains Goat Placenta again
Supernatant, its bioactivity of holding permanently effective at low temperature.
Claims (10)
- A kind of 1. Goat Placenta enzymatic hydrolysis and fermentation concentrate for cosmetic material, it is characterised in that：Sheep for cosmetic material Placenta enzymatic hydrolysis and fermentation concentrate includes frozen dry powder of sheep placenta, trypsase, papain, aspergillus niger and water.
- 2. it is used for the Goat Placenta enzymatic hydrolysis and fermentation concentrate of cosmetic material as claimed in claim 1, it is characterised in that：For changing The Goat Placenta enzymatic hydrolysis and fermentation concentrate of cosmetic raw material is by aspergillus niger, frozen dry powder of sheep placenta, trypsase, papain and water It is successively 1 according to weight ratio:50:80:20:1000 enzymatic hydrolysis and fermentations that feed intake are made.
- 3. it is used for the Goat Placenta enzymatic hydrolysis and fermentation concentrate of cosmetic material as claimed in claim 1 or 2, it is characterised in that：With The water used in Goat Placenta enzymatic hydrolysis and fermentation concentrate in cosmetic material is purified water, and the mesh number of frozen dry powder of sheep placenta used is The mesh of 20 mesh~50.
- A kind of 4. preparation technology of Goat Placenta enzymatic hydrolysis and fermentation concentrate for cosmetic material, it is characterised in that：For making up The preparation technology of the Goat Placenta enzymatic hydrolysis and fermentation concentrate of product raw material comprises the following steps that：Step 1, it is standby that Goat Placenta is prepared into freeze-dried powder；Step 2, quantitative frozen dry powder of sheep placenta is dissolved with water, and centrifugal treating, supernatant is collected, residue is standby；Step 3, aspergillus niger culture medium is prepared, the residue in step 2 is added into water, is configured to aspergillus niger culture medium, Ran Houjin The processing of row high-temperature sterilization；Step 4, the aspergillus niger culture medium in step 3 is recovered to 15 degrees Celsius~20 degrees Celsius, then to aspergillus niger culture Trypsase and papain are added in base, and is stirred, is then put into water-bath and digests, obtain enzymolysis liquid；Step 5, by the enzymolysis liquid autoclaving in step 4, its temperature is then set to revert to 15 degrees Celsius to 25 degrees Celsius, so After add aspergillus niger, stir, temperature be 28 degrees Celsius in the environment of ferment, then every 24 hours sampling once；Step 6, the zymotic fluid obtained in step 5 is subjected to centrifugal treating, then collects supernatant；Step 7, the attenuating that the supernatant in step 6 is carried out to conductance are handled, the solution rotary evaporation that then will be obtained Instrument is concentrated, and obtains the Goat Placenta enzymatic hydrolysis and fermentation concentrate for cosmetic material, and the Goat Placenta enzymatic hydrolysis and fermentation concentrate can use In the composition of raw materials of cosmetics.
- 5. it is used for the preparation technology of the Goat Placenta enzymatic hydrolysis and fermentation concentrate of cosmetic material, its feature as claimed in claim 4 It is：In step 2, quantitative frozen dry powder of sheep placenta is dissolved with water, and during centrifugal treating, centrifugal treating once, The rotating speed of centrifugation is 2000-3000r/min, centrifugation time 4-10min.
- 6. it is used for the preparation technology of the Goat Placenta enzymatic hydrolysis and fermentation concentrate of cosmetic material, its feature as claimed in claim 4 It is：In step 3, aspergillus niger culture medium carries out high-temperature sterilization processing, enters horizontal high voltage in the environment of being 121 degrees Celsius in temperature Sterilizing 20 minutes.
- 7. it is used for the preparation technology of the Goat Placenta enzymatic hydrolysis and fermentation concentrate of cosmetic material, its feature as claimed in claim 4 It is：In step 4, the temperature digested in water-bath is 30 degrees Celsius~58 degrees Celsius, and enzymolysis time is 1 hour~3 small When.
- 8. it is used for the preparation technology of the Goat Placenta enzymatic hydrolysis and fermentation concentrate of cosmetic material, its feature as claimed in claim 4 It is：In step 5, by the enzymolysis liquid autoclaving in step 4, temperature control is 121 degrees Celsius, and time control is 20 points Clock, the aspergillus niger are added in the form of conidia powder.
- 9. it is used for the preparation technology of the Goat Placenta enzymatic hydrolysis and fermentation concentrate of cosmetic material, its feature as claimed in claim 4 It is：The zymotic fluid obtained in step 5 is subjected to centrifugal treating, the rotating speed of centrifugation is 2000-3000r/min, and centrifugation time is 4-10min。
- 10. the Goat Placenta enzymatic hydrolysis and fermentation concentrate for cosmetic material as described in claim 4 or 5 or 6 or 7 or 8 or 9 Preparation technology, it is characterised in that：In step 7, using D296 types anion and D61 types resin cation to upper in step 6 Clear liquid reduces the pretreatment of conductance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710784967.7A CN107412136A (en) | 2017-09-04 | 2017-09-04 | Goat Placenta enzymatic hydrolysis and fermentation concentrate and preparation technology for cosmetic material |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710784967.7A CN107412136A (en) | 2017-09-04 | 2017-09-04 | Goat Placenta enzymatic hydrolysis and fermentation concentrate and preparation technology for cosmetic material |
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| CN107412136A true CN107412136A (en) | 2017-12-01 |
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| CN201710784967.7A Pending CN107412136A (en) | 2017-09-04 | 2017-09-04 | Goat Placenta enzymatic hydrolysis and fermentation concentrate and preparation technology for cosmetic material |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107982143A (en) * | 2017-12-26 | 2018-05-04 | 银川凤仪堂生物工程有限公司 | Semen Cuscutae zymotic fluid and preparation process and the application in cosmetics |
| CN108165583A (en) * | 2017-12-26 | 2018-06-15 | 银川凤仪堂生物工程有限公司 | Matrimony vine enzymatic hydrolysis and fermentation liquid and preparation process and the application in cosmetics |
| CN110478305A (en) * | 2019-08-21 | 2019-11-22 | 银川凤仪堂生物工程有限公司 | A kind of preparation process and its application of sheep placenta extract |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1537465A (en) * | 2003-10-14 | 2004-10-20 | 大连轻工学院 | Functional food contg. ewe placenta, and its prepn. method |
| CN104367592A (en) * | 2014-11-19 | 2015-02-25 | 杨公明 | New application of sheep placenta extract in treatment of skin burn and trauma |
| CN105434472A (en) * | 2015-11-19 | 2016-03-30 | 界首市菁华科技信息咨询服务有限公司 | Method for extracting sheep placenta extract primary liquid from sheep placentas |
| CN105982831A (en) * | 2015-02-09 | 2016-10-05 | 郭宗卫 | Cosmetic containing active peptide |
| CN106434803A (en) * | 2016-09-09 | 2017-02-22 | 江南大学 | Sheep placenta antioxidant polypeptide as well as enzymatic hydrolysis preparation method and application thereof |
-
2017
- 2017-09-04 CN CN201710784967.7A patent/CN107412136A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1537465A (en) * | 2003-10-14 | 2004-10-20 | 大连轻工学院 | Functional food contg. ewe placenta, and its prepn. method |
| CN104367592A (en) * | 2014-11-19 | 2015-02-25 | 杨公明 | New application of sheep placenta extract in treatment of skin burn and trauma |
| CN105982831A (en) * | 2015-02-09 | 2016-10-05 | 郭宗卫 | Cosmetic containing active peptide |
| CN105434472A (en) * | 2015-11-19 | 2016-03-30 | 界首市菁华科技信息咨询服务有限公司 | Method for extracting sheep placenta extract primary liquid from sheep placentas |
| CN106434803A (en) * | 2016-09-09 | 2017-02-22 | 江南大学 | Sheep placenta antioxidant polypeptide as well as enzymatic hydrolysis preparation method and application thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107982143A (en) * | 2017-12-26 | 2018-05-04 | 银川凤仪堂生物工程有限公司 | Semen Cuscutae zymotic fluid and preparation process and the application in cosmetics |
| CN108165583A (en) * | 2017-12-26 | 2018-06-15 | 银川凤仪堂生物工程有限公司 | Matrimony vine enzymatic hydrolysis and fermentation liquid and preparation process and the application in cosmetics |
| CN110478305A (en) * | 2019-08-21 | 2019-11-22 | 银川凤仪堂生物工程有限公司 | A kind of preparation process and its application of sheep placenta extract |
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Application publication date: 20171201 |
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