CN110478305A - A kind of preparation process and its application of sheep placenta extract - Google Patents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
- A61K8/982—Reproductive organs; Embryos, Eggs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The invention belongs to cosmetics processing technique fields, are related to the preparation process and its application of a kind of sheep placenta extract.The preparation process of the sheep placenta extract, comprising: 1. using sheep embryo and Goat Placenta as raw material, is successively cleaned, is rubbed and ultrasonication；2. complex enzyme formulation is added, pH is adjusted to 3~8 and is digested, then carries out destroy the enzyme treatment；3. sequentially add stress saccharomycete, glucose ferment, and keep the temperature, using centrifugal treating, collect supernatant, filtering obtains sheep placenta extract.The sheep placenta extract, by first digest, recycle stress yeast-leavened mode, obtain have maintenance skin, extra-nutrition, enhancing moisturizing, delay senescence and other effects sheep placenta.
Description
Technical field
The invention belongs to cosmetics processing technique fields, are related to the preparation process and its application of a kind of sheep placenta extract.
Background technique
Skin care item are the major products that current people maintain skin, for conventional toner, lotion, face cream,
The Essence concentration extraction refinement portion of product has more efficient skin care effect.Sheep placenta is from sheep embryo and sheep placenta
The compound containing a variety of active ingredients extracted in disk, the effective component of sheep placenta be small-molecular peptides, amino acid, protein,
Minerals, carbohydrate nucleic acid etc. can effectively repair the cell of damage, and the cell of decline is made to rejuvenate, and improve skin, eliminate
Wrinkle, recovery elasticity of skin, diminution pore, recovery skin gloss etc..
In existing process for extracting sheep placenta extract, all it is to directly adopt the mode of enzymatic hydrolysis to extract, is unable to fully obtain sheep
High activity sheep placenta in embryo, the skin-protection product being process also can not just play the role of efficiently improving skin；
Meanwhile the skin-protection product containing sheep placenta, functional component is often based on sheep placenta, so that also more single the effect of product；
Therefore, the extraction process of sheep placenta and specific practical application are all to be improved.
Summary of the invention
The purpose of the present invention is to provide a kind of preparation processes of sheep placenta extract, pass through the side for first digesting, fermenting again
Formula obtains high activity sheep placenta.
The object of the invention is also to provide a kind of applications of sheep placenta extract, by the way that the sheep placenta extract is added,
Obtain the sheep placenta Essence of moisturizing, anti-aging.
The technical solution that the present invention solves above-mentioned technical problem is as follows.
A kind of preparation process of sheep placenta extract, includes the following steps:
(1) it using sheep embryo and Goat Placenta as raw material, successively cleaned, rubbed and ultrasonication.
(2) complex enzyme formulation is added, pH is adjusted to 3~8 and is digested, then carries out destroy the enzyme treatment；Wherein, complex enzyme system
Agent includes one of trypsase, papain, lipase, neutral proteinase, bromelain or chymotrypsin or several
Kind；When enzymolysis processing, temperature is 30 DEG C~60 DEG C, the time is 1h~8h, and the dosage of complex enzyme formulation is 0~5%；Destroy the enzyme treatment
When, temperature is 60 DEG C~90 DEG C, the time is 20min~50min.
By above-mentioned enzymolysis process, specifically, by selecting above-mentioned complex enzyme formulation, and use suitable proportion and addition
Ratio, by the proteolysis of macromolecular at peptide fragment and partial amino-acid.
(3) sequentially add stress saccharomycete, glucose ferment, and keep the temperature, using centrifugal treating, collect supernatant
Liquid obtains sheep placenta extract；Wherein, stress the preparation process of saccharomycete be, by saccharomycete by ultraviolet light irradiation culture 2h
~5h, using spreading cultivation, freezing, drying, acquisition stress saccharomycete；Stress saccharomycete dosage be 0.05%~0.2%；Grape
The dosage of sugar is 0.1%~1%；When fermentation process, the time is 10h~18h；When isothermal holding, temperature be 90 DEG C~100 DEG C,
Time is 20min~50min；When centrifugal treating, centrifugal treating 5min~20min under the revolving speed of 5000r/min.
It, cannot be thoroughly by the protein breakdown of macromolecular at the object of small molecule since enzymolysis process can only digest fixed sequence
Matter, thus fermenting again by above-mentioned saccharomycete, can further progress decomposition.Importantly, what above-mentioned fermentation process used
Be stress saccharomycete, this stress saccharomycete, ultraviolet light irradiation in the case where grow, the bacterium finally to live to ultraviolet light have one
Fixed repellence, and the essence resisted is that saccharomycete produces the albumen of energy self-regeneration or can support anti-ultraviolet albumen；Make
With this stress saccharomycete, and grown by nutrients of sheep placenta, can not only decompose part macromolecular sheep placenta, can also generate
Some maintenance skin and anti-ultraviolet albumen is supported, sheep placenta fermentation liquid obtained providing the same of nutritional ingredient for skin
When, it can also repair by the skin of ultraviolet ray burn, effect is very complete.
A kind of application of sheep placenta extract is used to prepare the sheep placenta Essence that pH is 5~7.
The sheep placenta Essence, the raw material including following mass fraction:
10~95 parts of sheep placenta extract, 0.5~50 part of purslane extract, 0.5~20 part of pea seed extract, fructus lycii
0.5~20 part of extract, 0.1~2 part of thickener, 3~10 parts of moisturizer, 0.1~1.5 part of pH adjusting agent, essence 0.01~1
Part, 1~50 part of deionized water；Wherein, moisturizer includes glycerol, propylene glycol, polyethylene glycol, sorbierite, glycine betaine, hyaluronic acid
Or one or more of collagen；Thickener includes sodium alginate, hydroxyethyl cellulose, carbomer, acrylate or hydroxyl
One or more of propyl cellulose.
The sheep placenta Essence, further includes antioxidant；Antioxidant includes vitamin A, vitamin C, vitamin E or dimension
One or more of raw element D.
In above-mentioned sheep placenta Essence, sheep placenta extract contains small-molecular peptides, amino acid, protein, minerals, carbohydrate
The effective components such as nucleic acid can repair the cell of damage, make decline cell rejuvenate, improve skin, smooth away wrinkles,
Restore elasticity of skin, reduces pore, restores skin gloss.
Containing alkaloids in purslane extract, flavones, saponin, plant polyose and multivitamin etc. can be moistened with nutrition
Sliding skin, the cuticula of dead skin and generation caused by reducing due to drying；Flavones and saponin can remove free radical, inhibit junket ammonia
The aging of the activity, delaying skin of sour enzyme；Alkaloid and flavones can prevent skin with antibacterial and inhibition Common Pathogenic dermatophyte
Skin allergy repairs skin, disappears and red goes the effects of swollen.
Pea seed extract contains the various active materials such as flavones, amino acid, small-molecule peptide, can be effectively improved human body
Skin, beautifying whitening enhance skin elasticity, keep moisture of skin, prevent wrinkle from generating.
Flavones rich in, polysaccharide, protein, amino acid, carrotene, vitamin B in wolfberry fruit extract1, dimension life
Plain B2, niacin, vitamin C, the ingredients such as glycine betaine, there is anti-aging, antibacterial anti-inflammatory, whitening to dispel for fructus lycii flavones and polysaccharides
Spot, removing free radical and other effects.
The preparation process of sheep placenta extract of the present invention and its having the beneficial effect that for application, by first digesting, recycling and answer
Swash yeast-leavened mode, obtains the sheep placenta of high activity；The sheep placenta has maintenance skin, extra-nutrition, enhancing moisturizing, prolongs
Slow aging and other effects；By sheep placenta extract, in conjunction with other efficient skin care extracts, the Essence etc. being process has more
Add efficient moisturizing, anti-aging, crease-resistant, Bearberry Extract.
Specific embodiment
Embodiment 1
A kind of preparation process of sheep placenta extract, includes the following steps:
(1) sheep embryo and Goat Placenta are put into purified water and are cleaned, remove impurity and watery blood, then twisted with meat grinder
It is broken, watery blood is removed by ultrasonic cleaning.
(2) trypsase, papain, lipase, neutral proteinase, bromelain and chymotrypsin composition are selected
Complex enzyme formulation, and it is added according to rub raw material 3% into the rubbing raw material of sheep embryo and Goat Placenta, then by pH tune
To 3~8,5h~6h is digested at 30 DEG C~60 DEG C；After enzymatic hydrolysis, then at 60 DEG C~90 DEG C carry out 30min destroy the enzyme treatment.
(3) in the raw material Jing Guo destroy the enzyme treatment, sequentially add the 0.1% of its quality stress saccharomycete, add
0.5% glucose, and be uniformly mixed, ferment 12h~16h；After keeping the temperature 30min at 90 DEG C~100 DEG C again, by turning
Speed is that 5000r/min centrifuge carries out centrifugal treating 10min, collects supernatant, obtains sheep placenta extract.
Embodiment 2
A kind of preparation process of sheep placenta extract, includes the following steps:
(1) sheep embryo and Goat Placenta are put into purified water and are cleaned, remove impurity and watery blood, then twisted with meat grinder
It is broken, watery blood is removed by ultrasonic cleaning.
(2) the complex enzyme system of trypsase, papain, lipase, bromelain and chymotrypsin composition is selected
Agent, and it is added according to rub raw material 4% and is adjusted to 3~8, In into the rubbing raw material of sheep embryo and Goat Placenta, then by pH
4h~7h is digested at 30 DEG C~60 DEG C；After enzymatic hydrolysis, then at 60 DEG C~90 DEG C carry out 30min destroy the enzyme treatment.
(3) in the raw material Jing Guo destroy the enzyme treatment, sequentially add the 0.15% of its quality stress saccharomycete, add
0.5% glucose, and be uniformly mixed, ferment 12h~16h；After keeping the temperature 30min at 90 DEG C~100 DEG C again, by turning
Speed is that 5000r/min centrifuge carries out centrifugal treating 15min, collects supernatant, obtains sheep placenta extract.
Embodiment 3
A kind of preparation process of sheep placenta extract, includes the following steps:
(1) sheep embryo and Goat Placenta are put into purified water and are cleaned, remove impurity and watery blood, then twisted with meat grinder
It is broken, watery blood is removed by ultrasonic cleaning.
(2) trypsase, papain, lipase, neutral proteinase, bromelain and chymotrypsin composition are selected
Complex enzyme formulation, and it is added according to rub raw material 5% into the rubbing raw material of sheep embryo and Goat Placenta, then by pH tune
To 3~8,6h~8h is digested at 30 DEG C~60 DEG C；After enzymatic hydrolysis, then at 60 DEG C~90 DEG C carry out 40min destroy the enzyme treatment.
(3) in the raw material Jing Guo destroy the enzyme treatment, sequentially add the 0.15% of its quality stress saccharomycete, add
0.8% glucose, and be uniformly mixed, ferment 14h~18h；After keeping the temperature 40min at 90 DEG C~100 DEG C again, by turning
Speed is that 5000r/min centrifuge carries out centrifugal treating 10min, collects supernatant, obtains sheep placenta extract.
Embodiment 4
In the step of above-described embodiment 1 to embodiment 3 (3), stress the preparation process of saccharomycete be, by saccharomycete by purple
Outside line irradiation culture 3h, acquisition have certain flora for resisting UV resistance, using spreading cultivation, freezing, drying, are answered
Swash the freeze-dried powder of saccharomycete, this flora passes through verification experimental verification without bad mutation, securely and reliably.
Embodiment 5
A kind of application of sheep placenta extract is used to prepare the sheep placenta Essence that pH is 5~7.
Specifically, the sheep placenta Essence, is extracted including 80 parts of sheep placenta extract prepared by embodiment 1, purslane
40 parts of object, 20 parts of pea seed extract, 5 parts of wolfberry fruit extract, 1 part of thickener, 8 parts of moisturizer, 1 part of pH adjusting agent, essence 0.5
Part, 30~50 parts of deionized water.
Wherein, moisturizer includes glycerol, propylene glycol, polyethylene glycol, sorbierite, glycine betaine, hyaluronic acid and collagen；
Thickener includes sodium alginate, hydroxyethyl cellulose, carbomer, acrylate and hydroxypropyl cellulose.The sheep placenta Essence,
It further include antioxidant；Antioxidant includes vitamin A, vitamin C, vitamin E and vitamin D.
Embodiment 6
A kind of application of sheep placenta extract is used to prepare the sheep placenta Essence that pH is 5~7.
Specifically, the sheep placenta Essence, is extracted including 70 parts of sheep placenta extract prepared by embodiment 2, purslane
30 parts of object, 5 parts of pea seed extract, 10 parts of wolfberry fruit extract, 2 parts of thickener, 3 parts of moisturizer, 0.5 part of pH adjusting agent, essence
0.03 part, 20~50 parts of deionized water.
Wherein, moisturizer includes glycerol, propylene glycol, polyethylene glycol, sorbierite, glycine betaine, hyaluronic acid and collagen；
Thickener includes sodium alginate, hydroxyethyl cellulose, carbomer, acrylate and hydroxypropyl cellulose.The sheep placenta Essence,
It further include antioxidant；Antioxidant includes vitamin A, vitamin C, vitamin E and vitamin D.
Embodiment 7
In embodiment 5 and embodiment 6, the preparation method of the sheep placenta Essence includes:
(1) skin condition for forming sheep placenta extract, purslane extract, pea seed extract, wolfberry fruit extract
Agent and moisturizer, essence, antioxidant are dissolved in portions of de-ionized water；
(2) thickener and remaining deionized water are mixed；
(3) mixture that step (1) obtains and the mixture that step (2) obtains are mixed, then with pH adjusting agent tune
The pH for saving product reaches the numerical value 5~7 of relevant regulations that meet national standards, obtains sheep placenta Essence.
Wherein, in step (1), for dissolving skin conditioning agent, moisturizer, the use of the deionized water of antioxidant, essence
Amount is not particularly limited, as long as can be dissolved the skin conditioning agent of corresponding amount, moisturizer, antioxidant, essence；
For example, the amount of deionized water can be skin conditioning agent, moisturizer, antioxidant, one times of essence total weight.
In addition, sheep placenta Essence of the invention can be applied to various cosmetics, and e.g., the sheep based on sheep placenta Essence
Tire element essence facial mask, sheep placenta ampulla essence, sheep placenta ferritic film, sheep placenta essence face cream or sheep placenta essence cream etc., application
Field is very extensive.
Prepared sheep placenta extract and sheep placenta Essence through the foregoing embodiment have maintenance skin, Replacement Battalion
It supports, enhance the multi-efficiencies such as moisturizing, anti-aging, be suitable for thering are the various people of skin care demand to use.
Claims (10)
1. a kind of preparation process of sheep placenta extract, which comprises the steps of:
(1) it using sheep embryo and Goat Placenta as raw material, successively cleaned, rubbed and ultrasonication；
(2) complex enzyme formulation is added, pH is adjusted to 3~8 and is digested, then carries out destroy the enzyme treatment；
(3) sequentially add stress saccharomycete, glucose ferment, and keep the temperature, using centrifugal treating, collect supernatant, mistake
Filter obtains sheep placenta extract.
2. the preparation process of sheep placenta extract as described in claim 1, which is characterized in that in the step (2), complex enzyme
Preparation include one of trypsase, papain, lipase, neutral proteinase, bromelain or chymotrypsin or
It is several.
3. the preparation process of sheep placenta extract as described in claim 1, which is characterized in that in the step (2), at enzymatic hydrolysis
When reason, temperature is 30 DEG C~60 DEG C, the time is 1h~8h, and the dosage of complex enzyme formulation is 0~5%.
4. the preparation process of sheep placenta extract as described in claim 1, which is characterized in that in the step (2), at enzyme deactivation
When reason, temperature is 60 DEG C~90 DEG C, the time is 20min~50min.
5. the preparation process of sheep placenta extract as described in claim 1, which is characterized in that, stress ferment in the step (3)
The dosage of female bacterium is 0.05%~0.2%；The dosage of glucose is 0.1%~1%.
6. the preparation process of sheep placenta extract as described in claim 1, which is characterized in that in the step (3), at fermentation
When reason, the time is 10h~18h；When isothermal holding, temperature is 90 DEG C~100 DEG C, the time is 20min~50min；Centrifugal treating
When, centrifugal treating 5min~20min under the revolving speed of 5000r/min.
7. the preparation process of sheep placenta extract as described in claim 1, which is characterized in that it is described stress saccharomycete preparation
Technique is, by saccharomycete by ultraviolet light irradiation culture 2h~5h, using spreading cultivation, freezing, drying, acquisition stress saccharomycete.
8. a kind of application of sheep placenta extract, which is characterized in that be used to prepare the sheep placenta Essence that pH is 5~7；
The sheep placenta Essence, the raw material including following mass fraction:
10~95 parts of sheep placenta extract as described in any one of claims 1 to 7；
0.5~50 part of purslane extract, 0.5~20 part of pea seed extract, 0.5~20 part of wolfberry fruit extract, thickener 0.1
~2 parts, 3~10 parts of moisturizer, 0.1~1.5 part of pH adjusting agent, 0.01~1 part of essence, 1~50 part of deionized water.
9. the application of sheep placenta extract as claimed in claim 8, which is characterized in that the sheep placenta Essence, further includes resisting
Oxidant, the antioxidant include one or more of vitamin A, vitamin C, vitamin E or vitamin D.
10. the application of sheep placenta extract as claimed in claim 8 or 9, which is characterized in that the moisturizer include glycerol,
One or more of propylene glycol, polyethylene glycol, sorbierite, glycine betaine, hyaluronic acid or collagen；The thickener includes
One or more of sodium alginate, hydroxyethyl cellulose, carbomer, acrylate or hydroxypropyl cellulose.
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| CN111035595A (en) * | 2019-11-26 | 2020-04-21 | 银川凤仪堂生物工程有限公司 | Preparation method of coarse grain complex enzymatic hydrolysis leavening and application of coarse grain complex enzymatic hydrolysis leavening in skin care products |
| CN111820404A (en) * | 2020-07-07 | 2020-10-27 | 肖启森 | Sheep embryo extract beautifying oral liquid and preparation method thereof |
| CN112315892A (en) * | 2020-12-10 | 2021-02-05 | 西藏天慈生物科技有限公司 | Sheep placenta anti-wrinkle whitening freeze-dried powder and method |
| CN113577005A (en) * | 2021-08-02 | 2021-11-02 | 京美瑞禾健康科技(辽宁)有限公司 | Sheep placenta ultramicro factor mask liquid and preparation method thereof |
| CN115517996A (en) * | 2022-04-25 | 2022-12-27 | 广州市万千粉丝化妆品有限公司 | Application of exosome in animal placenta fermentation cell-mesenchymal filtrate in maintenance product |
| CN114766619A (en) * | 2022-05-30 | 2022-07-22 | 中山市美太保健制品有限公司 | Health-care product oral liquid for delaying senility and preparation method thereof |
| CN115300443A (en) * | 2022-08-02 | 2022-11-08 | 银川凤仪堂生物工程有限公司 | Sheep placenta extract extraction method and application thereof in cosmetics |
| CN115300443B (en) * | 2022-08-02 | 2023-12-08 | 银川凤仪堂生物工程有限公司 | Sheep embryo extract extraction method and application thereof in cosmetics |
| KR102503124B1 (en) * | 2022-08-18 | 2023-02-23 | 주식회사 에이바이오머티리얼즈 | Method for preparing sheep placenta extract containing high content of amino acid and cosmetic composition comprising the extract |
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