CN109943614A - A kind of preparation method and applications of Goat Placenta active peptide - Google Patents
A kind of preparation method and applications of Goat Placenta active peptide Download PDFInfo
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- CN109943614A CN109943614A CN201910261777.6A CN201910261777A CN109943614A CN 109943614 A CN109943614 A CN 109943614A CN 201910261777 A CN201910261777 A CN 201910261777A CN 109943614 A CN109943614 A CN 109943614A
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Abstract
The present invention relates to a kind of Goat Placenta active peptide preparation method and applications, the preparation method specifically: Goat Placenta is cleaned and carries out being concentrated after autoclaving, rubbing, enzymatic hydrolysis, enzyme deactivation, filtering after cleaning being made, Goat Placenta active peptide obtained can be made into various skin care item, water shortage, bright color, alleviation skin aging are had dry skin applied to improving, preparation method simple possible of the present invention, at low cost, process stabilizing, yield are high, and obtained product purity and quality is high.
Description
Technical field
The invention belongs to daily use chemicals technical field more particularly to a kind of preparation methods of Goat Placenta active peptide.
Background technique
China is an animal husbandry big country, and with the development of China's livestock and poultry breeding industry, the produced by-product of slaughtering is then
It can not be ignored.Goat Placenta is cheap and easy to get as a kind of extensive resource.Goat Placenta wherein protein rich in, kind amino acid,
Kind microelement, phosphatide, lipopolysaccharides, vitamin, there are also various active polypeptides relevant to immune function and body normal operation.
The functional characteristic of placenta is increasingly recognized by people, and therefrom the active peptide of extraction functionality has wide development prospect.
Chinese patent CN 108866136A discloses a kind of preparation method of low molecular polypeptide, and the method discloses one kind
The preparation method of low molecular polypeptide, feature are that the enzymolysis and extraction technology used realizes depth extraction to Goat Placenta, using having
The filtering of machine filter membrane, obtains low molecular polypeptide.Patent CN 108771638A be related to a kind of biomass placenta freeze-dried powder preparation and its
Production method, the patent include the pulvis being made of sheep placenta and the solution that is made of placenta freeze-dried powder lyase liquid, can be subtracted
The scars such as acne scar are repaired in the generation of few wrinkle, the aging of delaying skin, are repaired skin trauma and are promoted wound healing, have and add
It is strong immune repair, be anti-inflammatory, antianaphylactic effect.Patent CN 107216366A discloses a kind of preparation of Goat Placenta small-molecular peptides
Method and its application, the extracting method include pre-treatment, crushing, broken wall, centrifugation, ultrafiltration, freeze-drying, separating twice.
At present about Goat Placenta as peptide source product based on oral solution, the sheep placenta in Goat Placenta is for the human body
It is foreign preteins, molecular weight, which is greater than 10000D, has certain anaphylaxis, and most of extracting methods are filtered by film, and it is at high cost,
Process is complicated.The present invention prepares Goat Placenta active peptide method simple possible, at low cost, process stabilizing, yield height with enzymatic isolation method, obtains
The product purity and quality arrived is high.For this status, applicant invents a kind of preparation of Goat Placenta active peptide through system research
Method simultaneously studies its application.Goat Placenta active peptide can be made into various skin care item, applied to improvement dry skin water shortage, highlight skin
Color alleviates skin aging.
Summary of the invention
The present invention overcomes the shortcoming of the prior art and product, invents a kind of simple possible, at low cost, process stabilizing
The preparation method of Goat Placenta active peptide, and skin care item are made using the Goat Placenta active peptide.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of preparation method of Goat Placenta active peptide, comprising the following steps:
(1) thermophilic digestion: Goat Placenta and water are mixed in the ratio of 1:3-1:6, are put into high-pressure sterilizing pot after mixing evenly
It is interior, with 121 DEG C autoclaving 20-50 minutes.
(2) it rubs: the Goat Placenta of autoclaving after-tack is rubbed using meat grinder.Every 10s is once rubbed
Processing is rubbed the time in 5S or so each time, is executed 6-8 times.
(3) it digests: under conditions of pH is 4-10.5, being digested 5-9 hours using protease in 35-40 DEG C of heat preservation.It is described
Protease includes acid protease, neutral proteinase and alkali protease.
(4) enzyme deactivation: in high-pressure sterilizing pot, with 121 DEG C high pressure enzyme deactivation 20-30 minutes.
(5) it filters: using being filtered after activated carbon adsorption.
(6) be concentrated: heating temperature be 50-60 DEG C, 20-25 DEG C of coolant temperature, pressure 2-3 KPa under conditions of into
Row rotary evaporation in vacuo.
A kind of application of the skin care item containing Goat Placenta active peptide, the cosmetic applications in improve dry skin water shortage,
Bright color alleviates skin aging.
Further, the cosmetics are to can be toner, lotion, face cream, Essence, facial mask or Sprayable.
Further, the cosmetics also include the acceptable auxiliary material of cosmetic field, including but not limited to preservative, antioxygen
Agent, moisturizer, thickener, intercalating agent, stabilizer and emulsifier.
The advantages and positive effects of the present invention are:
1, Goat Placenta active peptide of the present invention is prepared by enzymatic isolation method, this method simple possible, at low cost, technique
Stablize, yield height, obtained product purity and quality is high.Goat Placenta resource is extensive, it is cheap and easy to get and be rich in multiple proteins.
2, Goat Placenta is using being obtained Goat Placenta active peptide by the present invention after digesting, then with other raw materials (preservative, antioxygen
Agent, moisturizer, thickener, intercalating agent, stabilizer and emulsifier) mixing, Goat Placenta active peptide can be scarce with improvement dry skin
Water, alleviates skin aging at bright color.
Specific embodiment
The present invention is further described below by specific embodiment, it is not limit that following embodiment, which is descriptive,
Qualitatively, this does not limit the scope of protection of the present invention.
Embodiment 1:
A kind of Goat Placenta active peptide Essence with anti-aging effects, raw material are by mass percentage, including as follows
Component Goat Placenta active peptide 40%；Niacinamide 2%；Ascorbic acid glucoside 2%；Glycerol 2%；Deionized water 40%；EDTA bis-
Sodium 2%；Tocopherol 4%；Xanthan gum 2%；Sodium Hyaluronate 5%；Phenoxyethanol 1%.
The preparation method of above-mentioned Goat Placenta active peptide Essence comprising following steps:
(1) goat placenta is cleaned and is cleaned, be placed at shady and cool ventilation and save.
(2) Goat Placenta after cleaning is mixed with water according to 1:6, is put into high-pressure sterilizing pot after mixing evenly with 121 DEG C
Condition handle 20min.
(3) material is rubbed using meat grinder.Every 10s carries out primary rubbing processing, rubs time 5S each time, holds
Row 6 times.
(4) it under conditions of pH is 7, is digested 7 hours using neutral proteinase in 38 DEG C of heat preservations.
(5) raw material after enzymatic hydrolysis is subjected to destroy the enzyme treatment, enzyme deactivation condition is 121 DEG C, 20min.
(6) impurity is filtered off using being filtered after activated carbon adsorption after enzyme deactivation.
(7) filtered enzymolysis liquid is subjected to vacuum concentrated by rotary evaporation, technological parameter is that heating temperature is 50 DEG C, cooling medium
20 DEG C of temperature, 2 KPa of pressure, obtain Goat Placenta active peptide.
(8) each component is weighed by proportion described in the present embodiment raw material, by Goat Placenta active peptide, hyaluronic acid, ascorbic acid
It is stand-by after mixing in temperature 50 C that glucoside, niacinamide and tocopherol are dissolved in a small amount of deionized water, obtains premixed liquid.It will
Xanthan gum, EDETATE SODIUM and glycerol are placed in the balance deionized water that temperature is 70 DEG C after swelling, and Phenoxyethanol is added and is stirring
Dissolution is stirred well on the blender that speed is 2000r/min to be uniformly mixed to get Goat Placenta active peptide Essence.
Embodiment 2:
A kind of Goat Placenta activity peptide facial mask with anti-inflammatory anti-acne effect, raw material by mass percentage, including such as
Lower component Goat Placenta active peptide powder 2%；Niacinamide 1.5%；Asparagus juice 1.5%；Glycerol 5%；Deionized water 77%；EDETATE SODIUM
2%；Tocopherol acetate 3%；Xanthan gum 2%；Butanediol 5%；Streptococcus lactis 0.5%；Phenoxyethanol 0.5%.
The preparation method of above-mentioned Goat Placenta activity peptide facial mask comprising following steps:
(1) sheep placenta is cleaned and is cleaned, be placed at shady and cool ventilation and save.
(2) Goat Placenta after cleaning is mixed with water according to 1:3, is put into high-pressure sterilizing pot after mixing evenly with 121 DEG C
Condition handle 20min.
(3) material is rubbed using meat grinder.Every 10s carries out primary rubbing processing, rubs time 6S each time, holds
Row 7 times.
(4) it under conditions of pH is 4, is digested 5 hours using acid protease in 40 DEG C of heat preservations.
(5) raw material after enzymatic hydrolysis is subjected to destroy the enzyme treatment, enzyme deactivation condition is 121 DEG C, 20min.
(6) impurity is filtered off using being filtered after activated carbon adsorption after enzyme deactivation.
(7) filtered enzymolysis liquid liquid is spray-dried, technological parameter is that inlet air temperature is 150 DEG C, homogenization pressure
30MPa, 60 DEG C of leaving air temp is to get arriving Goat Placenta active peptide powder.
(8) each component is weighed by proportion described in the present embodiment raw material, by Goat Placenta active peptide powder, asparagus juice, nisin
Bacterium, niacinamide, tocopherol acetate and butanediol are stand-by after mixing in temperature 45 C in a small amount of deionized water, obtain
Premixed liquid.Xanthan gum, EDETATE SODIUM and glycerol are placed in the balance deionized water that temperature is 75 DEG C after being swollen, benzene oxygen second is added
Alcohol low whipping speed be 2000r/min blender on be stirred well to dissolution be uniformly mixed, be cooled to 45 DEG C be added it is above-mentioned to
With premixed liquid to get Goat Placenta activity peptide facial mask.
Embodiment 3:
A kind of Goat Placenta active peptide toner with moistening effect, raw material by mass percentage, including such as the following group
Divide Goat Placenta active peptide liquid 13%；Niacinamide 2%；Ascorbic acid glucoside 2%；Glycerol 5%；Deionized water 58%；EDTA bis-
Sodium 1%；Tocopherol 2%；Xanthan gum 1.5%；Hyaluronic acid 5%；Phenoxyethanol 0.5%.
The preparation method of above-mentioned Goat Placenta active peptide toner comprising following steps:
(1) sheep placenta is cleaned and is cleaned, be placed at shady and cool ventilation and save.
(2) Goat Placenta after cleaning is mixed with water according to 1:4, is put into high-pressure sterilizing pot after mixing evenly with 121 DEG C
Condition handle 20min.
(3) material is rubbed using meat grinder.Every 8s carries out primary rubbing processing, rubs time 7S each time, holds
Row 6 times.
(4) it under conditions of pH is 9.5, is digested 6 hours using alkali protease in 35 DEG C of heat preservations.
(5) raw material after enzymatic hydrolysis is subjected to destroy the enzyme treatment, enzyme deactivation condition is 121 DEG C, 20min.
(6) impurity is filtered off using being filtered after activated carbon adsorption after enzyme deactivation.
(7) enzymolysis solution after enzyme deactivation is subjected to vacuum concentrated by rotary evaporation, technological parameter is that heating temperature is 60 DEG C, cooling medium
30 DEG C of temperature, 3 KPa of pressure, obtain Goat Placenta active peptide liquid.
(8) each component is weighed by proportion described in the present embodiment raw material, by Goat Placenta active peptide liquid, hyaluronic acid, Vitamin C
It is stand-by after mixing in 40 DEG C of temperature that sour glucoside, niacinamide and tocopherol are dissolved in a small amount of deionized water, obtains premixed liquid.
Xanthan gum, EDETATE SODIUM and glycerol are placed in the balance deionized water that temperature is 60 DEG C after being swollen, Phenoxyethanol is added and is stirring
It mixes to be stirred well to dissolve on the blender that speed is 1500r/min and be uniformly mixed to get Goat Placenta active peptide toner.
Claims (7)
1. a kind of preparation method of Goat Placenta active peptide, it is characterised in that: the Goat Placenta active peptide is to be cleaned to remove by Goat Placenta
Concentration is carried out after miscellaneous after autoclaving, rubbing, enzymatic hydrolysis, enzyme deactivation, filtering to be made.
2. a kind of Goat Placenta active peptide preparation method according to claim 1, it is characterised in that: the Goat Placenta type
Are as follows: goat or sheep.
3. a kind of Goat Placenta active peptide preparation method according to claim 1, it is characterised in that: the autoclaving condition
Are as follows: by after removal of impurities Goat Placenta and water in 1:3-1:6 ratio mix, be put into high-pressure sterilizing pot after mixing evenly, with 121 DEG C
Autoclaving 20-50 minutes.
4. a kind of Goat Placenta active peptide preparation method according to claim 1, it is characterised in that: the condition of the enzymatic hydrolysis
Are as follows: under conditions of pH is 4-10.5, digested 5-9 hours using protease in 35-40 DEG C of heat preservation.
5. a kind of Goat Placenta active peptide preparation method according to claim 1, it is characterised in that: the protease includes acid
One of property protease, neutral proteinase and alkali protease are a variety of.
6. a kind of Goat Placenta active peptide preparation method according to claim 1, it is characterised in that: the condition of the filtering
Are as follows: using being filtered after activated carbon adsorption.
7. a kind of Goat Placenta active peptide preparation method according to claim 1, it is characterised in that: the Goat Placenta active peptide
It can be made into various skin care item, be applied to improve dry skin water shortage, bright color, alleviate skin aging.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113318060A (en) * | 2021-06-09 | 2021-08-31 | 上海南滨江细胞生物科技有限公司 | Essence containing embryo extract and production process thereof |
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| JP6213756B1 (en) * | 2017-02-09 | 2017-10-18 | 有限会社ポークランド | Method for producing placenta extract, method for producing placenta extract-containing powder, and method for producing processed food |
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2019
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Inventor after: Li Guilu Inventor after: Xia Guoqiang Inventor after: Lu Xiuyi Inventor before: Li Guilu Inventor before: Xia Guoqiang Inventor before: Lu Xiuyi |
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