CN113577005A - Sheep placenta ultramicro factor mask liquid and preparation method thereof - Google Patents
Sheep placenta ultramicro factor mask liquid and preparation method thereof Download PDFInfo
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- CN113577005A CN113577005A CN202110880272.5A CN202110880272A CN113577005A CN 113577005 A CN113577005 A CN 113577005A CN 202110880272 A CN202110880272 A CN 202110880272A CN 113577005 A CN113577005 A CN 113577005A
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- 241001494479 Pecora Species 0.000 title claims abstract description 94
- 210000002826 placenta Anatomy 0.000 title claims abstract description 84
- 239000007788 liquid Substances 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims description 18
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000012153 distilled water Substances 0.000 claims abstract description 19
- 239000000284 extract Substances 0.000 claims abstract description 13
- 229920002385 Sodium hyaluronate Polymers 0.000 claims abstract description 9
- 229940010747 sodium hyaluronate Drugs 0.000 claims abstract description 9
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims abstract description 9
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- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 7
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- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 claims description 7
- 239000004480 active ingredient Substances 0.000 claims description 6
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- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 claims description 5
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 4
- 239000004365 Protease Substances 0.000 claims description 4
- 229920002125 Sokalan® Polymers 0.000 claims description 4
- 229960001631 carbomer Drugs 0.000 claims description 4
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- MXOAEAUPQDYUQM-QMMMGPOBSA-N (S)-chlorphenesin Chemical compound OC[C@H](O)COC1=CC=C(Cl)C=C1 MXOAEAUPQDYUQM-QMMMGPOBSA-N 0.000 claims description 3
- 239000004354 Hydroxyethyl cellulose Substances 0.000 claims description 3
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- 235000010493 xanthan gum Nutrition 0.000 claims description 3
- 229940082509 xanthan gum Drugs 0.000 claims description 3
- 108090000145 Bacillolysin Proteins 0.000 claims description 2
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- 102000035092 Neutral proteases Human genes 0.000 claims description 2
- 108091005507 Neutral proteases Proteins 0.000 claims description 2
- 108090000526 Papain Proteins 0.000 claims description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 2
- 108090000631 Trypsin Proteins 0.000 claims description 2
- 102000004142 Trypsin Human genes 0.000 claims description 2
- 235000019835 bromelain Nutrition 0.000 claims description 2
- 229960002376 chymotrypsin Drugs 0.000 claims description 2
- 229940071826 hydroxyethyl cellulose Drugs 0.000 claims description 2
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 claims description 2
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 claims description 2
- 229960002216 methylparaben Drugs 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 235000019834 papain Nutrition 0.000 claims description 2
- 229940055729 papain Drugs 0.000 claims description 2
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- 238000003756 stirring Methods 0.000 claims description 2
- 239000012588 trypsin Substances 0.000 claims description 2
- 239000004367 Lipase Substances 0.000 claims 1
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- 239000002537 cosmetic Substances 0.000 claims 1
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- 230000009759 skin aging Effects 0.000 abstract description 5
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- 230000000694 effects Effects 0.000 description 14
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
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- 210000000056 organ Anatomy 0.000 description 2
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- 201000004624 Dermatitis Diseases 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
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- 238000011161 development Methods 0.000 description 1
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- 210000000987 immune system Anatomy 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
- A61K8/982—Reproductive organs; Embryos, Eggs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Abstract
The invention discloses an ovine placenta ultramicro factor mask solution which is characterized by comprising, by mass, 4-8 parts of an ovine placenta ultramicro factor, 1-3 parts of trehalose, 1-3 parts of a motherwort extract, 0.1-0.4 part of a preservative, 0.3-0.6 part of a thickening agent, 1-3 parts of glycerol, 1-3 parts of sodium hyaluronate, and the balance of distilled water. The sheep placenta ultramicro factor mask liquid disclosed by the invention can effectively delay skin aging and supplement skin moisture.
Description
The invention belongs to the field of medical cosmetology, and particularly relates to sheep placenta factor ultramicro facial mask liquid and a preparation method thereof.
Technical background:
the skin is the largest organ of the human body, is positioned on the surface of the human body, has the function of regulating the body temperature, and forms a protective barrier to protect the human body from microorganisms and toxicants. However, aging of human skin is a natural phenomenon, and especially facial skin is often exposed to the external environment for a long time, which easily causes damage to epidermal cells, causes the skin to become dry and lose elasticity, and may even cause skin inflammation, so that daily care of the skin of the face plate becomes a lesson that beauty lovers need to do everyday. The current work rhythm is accelerated, so that people can nurse the skin by more time of fragments, and therefore, the mask becomes a main daily means for caring the facial skin.
There are many facial masks on the market, wherein the main functional ingredients are contained in facial mask liquid, but most of the facial mask liquid on the market at present consists of water, a silicon smoothing agent, glycerol and other ingredients. And after the facial mask liquid is used every time, the facial mask liquid only has transient and superficial effects, can only transiently relieve the problem of skin water shortage, has a non-lasting effect and unobvious anti-aging and whitening effects, and the facial mask liquid containing anti-aging and moisturizing skin care agents in most uses has certain stimulation to the skin and can cause potential damage to the skin. Therefore, it is very important to develop a safe and effective facial mask solution for skin anti-aging, whitening and moisturizing.
Sheep placenta essence is rich in comprehensive active components for human immune system and organs. The sheep placenta extract contains immunoglobulin and aging delaying factor, and has good effect of delaying skin aging. Meanwhile, the protein content of the sheep placenta is more than 80%, the amino acid content is rich, the variety is complete, the proportion is reasonable, and the immune globulin and rich nutrient substances can ensure that the skin can obtain the secondary development like regeneration, so the sheep placenta is widely used as the effective components of the mask. However, since the molecular weight of the active substance in the sheep placenta is relatively large and the active substance is difficult to penetrate through the horny layer of the skin and enter the epidermal layer, the simple sheep placenta cannot effectively play the role of nourishing the skin, so that the sheep placenta active ingredient which is easily absorbed by the skin is developed and combined with other auxiliary materials to form the facial mask liquid, and the sheep placenta active ingredient has great market value.
The sheep placenta extract ultramicro factor is prepared by extracting active ingredients with a dose less than 5KD from sheep placenta extract by using a supercritical tangential flow filtration technology, so that the absorption of the sheep placenta extract on skin can be accelerated, the active ingredients can directly permeate into the deep layer of a skin substrate, the skin aging can be fundamentally relieved, and the skin moisture can be supplemented.
The invention content is as follows:
therefore, the invention discloses a sheep placenta factor ultramicro factor mask solution which is prepared by matching sheep placenta factor ultramicro factors with other auxiliary materials, and is beneficial to delaying skin aging and supplementing skin moisture.
The sheep placenta factor mask liquid is characterized by comprising, by mass, 4-8 parts of sheep placenta factor ultramicro factors, 1-3 parts of trehalose, 1-3 parts of motherwort herb extracts, 0.1-0.4 part of preservatives, 0.3-0.6 part of thickening agents, 1-3 parts of glycerol, 1-3 parts of sodium hyaluronate, and the balance of distilled water.
Further, the extraction method of the sheep placenta ultramicro factor comprises the following steps
Step one, collecting Tibetan sheep embryos which are pregnant for 18 to 20 weeks, and washing the Tibetan sheep embryos with distilled water for later use.
And step two, mincing the sheep placenta by using a meat mincer, and adding distilled water to dissolve the sheep placenta.
And step three, adding 0.5-1% of complex enzyme preparation at 45-50 ℃, reacting for 6-8h, and adjusting the pH value to 6.8-7.5.
And step four, filtering the sheep placenta solution, removing impurities, and collecting the sheep placenta ultramicro factor solution by using a tangential flow filter membrane.
Further, the molecular weight cut-off of the tangential flow filtration membrane is 4 KD-6 KD.
Further, the compound enzyme preparation comprises one or more of trypsin, papain, neutral protease, bromelain or chymotrypsin.
Further, the preservative is one or more of methylparaben, propylparaben and chlorphenesin.
Further, the thickening agent is one or more of hydroxyethyl cellulose, carbomer, xanthan gum and sodium acetate.
Further, the preparation method of the sheep placenta factor ultramicro-facial mask solution comprises the steps of mixing the components in proportion, and stirring for 3-5 hours at the temperature of 40-60 ℃ at the speed of 600-1000 r/min to obtain the sheep placenta factor ultramicro-facial mask solution.
The invention has the advantages that: the invention provides a sheep placenta factor ultramicro-factor mask solution, which is prepared by extracting bioactive components with the molecular weight of less than 5KD by using a supercritical tangential flow filtration technology, is easier to absorb by human skin, and combining the sheep placenta factor ultramicro-factor with other auxiliary materials into the mask solution, can radically relieve skin aging and supplement skin moisture, and has great market value.
The specific implementation mode is as follows:
in order to make the technical solution of the present invention better understood by those skilled in the art, the following provides a detailed description of the product of the present invention with reference to the examples.
Example 1
The sheep placenta factor ultramicro-factor mask liquid comprises the following components, by mass, 8 parts of sheep placenta factor ultramicro factors, 3 parts of trehalose, 3 parts of motherwort herb extracts, 0.4 part of methyl hydroxybenzoate, 0.6 part of hydroxyethyl cellulose, 3 parts of glycerol, 3 parts of sodium hyaluronate, and the balance of distilled water.
The extraction method of the sheep placenta ultramicro factor comprises the following steps of selecting fresh Tibetan sheep embryos which are pregnant for 18-20 weeks, and washing the Tibetan sheep embryos clean by using distilled water for later use. And step two, mincing the sheep placenta by using a meat mincer, and adding distilled water to dissolve the sheep placenta. And step three, adding 0.5-1% of complex enzyme preparation at 45-50 ℃, reacting for 6-8h, and adjusting the pH value to 6.8-7.5 by using NaOH solution. And step four, filtering the sheep placenta solution to remove large-particle impurities, and collecting the sheep placenta ultramicro factor solution by using a tangential flow filter membrane with the molecular weight cutoff of 4-6 KD.
Example 2
The sheep placenta factor ultramicro-factor mask solution comprises the following components, by mass, 4 parts of sheep placenta factor ultramicro factors, 2 parts of trehalose, 3 parts of motherwort herb extract, 0.4 part of propyl hydroxybenzoate, 0.6 part of carbomer, 3 parts of glycerol, 3 parts of sodium hyaluronate, and the balance of distilled water.
The extraction method of the sheep placenta ultramicro factor comprises the following steps of selecting fresh Tibetan sheep embryos which are pregnant for 18-20 weeks, and washing the Tibetan sheep embryos clean by using distilled water for later use. And step two, mincing the sheep placenta by using a meat mincer, and adding distilled water to dissolve the sheep placenta. And step three, adding 0.5-1% of complex enzyme preparation at 45-50 ℃, reacting for 6-8h, and adjusting the pH value to 6.8-7.5 by using NaOH solution. And step four, filtering the sheep placenta solution to remove large-particle impurities, and collecting the sheep placenta ultramicro factor solution by using a tangential flow filter membrane with the molecular weight cutoff of 4-6 KD.
Example 3
The sheep placenta factor ultramicro-factor mask liquid comprises the following components, by mass, 6 parts of sheep placenta factor ultramicro-factor, 1 part of trehalose, 2 parts of a motherwort extract, 0.4 part of propylparaben, 0.4 part of carbomer, 1 part of glycerol, 2 parts of sodium hyaluronate, and the balance of distilled water.
The extraction method of the sheep placenta ultramicro factor comprises the following steps of selecting fresh Tibetan sheep embryos which are pregnant for 18-20 weeks, and washing the Tibetan sheep embryos clean by using distilled water for later use. And step two, mincing the sheep placenta by using a meat mincer, and adding distilled water to dissolve the sheep placenta. And step three, adding 0.5-1% of complex enzyme preparation at 45-50 ℃, reacting for 6-8h, and adjusting the pH value to 6.8-7.5 by using NaOH solution. And step four, filtering the sheep placenta solution to remove large-particle impurities, and collecting the sheep placenta ultramicro factor solution by using a tangential flow filter membrane with the molecular weight cutoff of 4-6 KD.
Example 4
The sheep placenta factor ultramicro-factor mask solution comprises the following components, by mass, 7 parts of sheep placenta factor ultramicro factors, 3 parts of trehalose, 1 part of motherwort extract, 0.2 part of chlorphenesin, 0.6 part of xanthan gum, 3 parts of glycerol, 2 parts of sodium hyaluronate and the balance of distilled water.
The extraction method of the sheep placenta ultramicro factor comprises the following steps of selecting fresh Tibetan sheep embryos which are pregnant for 18-20 weeks, and washing the Tibetan sheep embryos clean by using distilled water for later use. And step two, mincing the sheep placenta by using a meat mincer, and adding distilled water to dissolve the sheep placenta. And step three, adding 0.5-1% of complex enzyme preparation at 45-50 ℃, reacting for 6-8h, and adjusting the pH value to 6.8-7.5 by using NaOH solution. And step four, filtering the sheep placenta solution to remove large-particle impurities, and collecting the sheep placenta ultramicro factor solution by using a tangential flow filter membrane with the molecular weight cutoff of 4-6 KD.
Comparative example 1
The formula and the preparation method of the sheep placenta factor ultramicro facial mask liquid are the same as those of example 1, except that sheep placenta factor ultramicro factors are not added.
Comparative example 2
The formula and the preparation method of the sheep placenta factor ultramicro facial mask liquid are the same as those in example 1, except that sodium hyaluronate is not added.
Comparative example 3
A sheep placenta factor ultramicro facial mask liquid has the same formula and preparation method as example 1, except that no herba Leonuri extract is added.
Comparative example 4
The recipe and preparation process of sheep placenta factor ultramicro facial mask liquid are the same as example 1, except that sheep placenta factor is filtered through filter membrane without being tangential.
In order to further verify the practical effect of the sheep placenta factor ultramicro facial mask liquid, skin quality improvement experiments are carried out on the sheep placenta factor ultramicro facial mask liquid in the examples and the comparative examples.
The experimental method comprises the following steps: selecting 120 test volunteers to carry out experiments, randomly dividing the test subjects into 8 groups, wherein each group comprises 15 persons, the groups correspond to the examples 1-4 and the comparative examples 1-4, the test person groups are objectively divided, the ingredients of the product are unknown, and the influence of psychological factors on the overall evaluation of the product is eliminated. 8 groups of subjects are coated with the sheep placenta ultramicro factor facial mask liquid of examples 1-4 and comparative examples 1-4 every day, the product effect is totally evaluated 2 times in the morning and at the evening, and the comprehensive effect evaluation of skin improvement is three grades, which are respectively: the effect is not obvious (0-3 min); the effect is general (4-6 points); the effect is obvious (7-10 minutes). The results are shown in Table 1.
As can be seen from the table 1, the sheep placenta factor ultramicro-factor facial mask liquid of the embodiment 1 of the invention has better skin quality improvement effect; compared with the comparative example 2 and the comparative example 1, the skin effect is remarkably improved after the content of the sheep placenta ultramicro factor is reduced by half or after the sheep placenta ultramicro factor is not added, which shows that the sheep placenta ultramicro factor plays a main role in the skin improvement effect experiment; compared with comparative examples 2-3, the sheep placenta ultramicro factor mask liquid without adding sodium hyaluronate and motherwort herb extract has slightly reduced improvement on skin quality, and shows that the skin quality improvement effect is the result of synergistic effect of each group of active ingredients. Compared with the comparative example 4, the skin quality improvement effect of the facial mask solution prepared from the sheep placenta without being treated by the ultramicro factor process is obviously reduced (compared with the example 1), which shows that the simple sheep placenta is not easily absorbed by the skin, and the skin quality improvement effect on the skin is not obvious. The sheep placenta factor is easier to be absorbed by the skin, and penetrates deep into the basal layer of the skin, so that the skin quality is better improved.
| Experimental group | Skin improvement effect score |
| Example 1 | 8.93 |
| Example 2 | 7.84 |
| Example 3 | 8.42 |
| Example 4 | 8.61 |
| Comparative example 1 | 4.86 |
| Comparative example 2 | 7.69 |
| Comparative example 3 | 8.01 |
| Comparative example 4 | 6.78 |
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (8)
1. The sheep placenta factor mask liquid is characterized by comprising, by mass, 4-8 parts of sheep placenta factor, 1-3 parts of trehalose, 1-3 parts of a motherwort extract, 0.1-0.4 part of a preservative, 0.3-0.6 part of a thickener, 1-3 parts of glycerol, 1-3 parts of sodium hyaluronate, and the balance of distilled water.
2. The amniocin ultramicro factor mask solution as claimed in claim 1, wherein the extraction method of amniocin ultramicro factor is
Step one, collecting Tibetan sheep embryos which are pregnant for 18 to 20 weeks, and washing the Tibetan sheep embryos with distilled water for later use.
And step two, mincing the sheep placenta by using a meat mincer, and adding distilled water to dissolve the sheep placenta.
And step three, adding 0.5-1% of complex enzyme preparation at 45-50 ℃, reacting for 6-8h, and adjusting the pH value to 6.8-7.5.
And step four, filtering the sheep placenta solution, removing impurities, and collecting the sheep placenta ultramicro factor solution by using a tangential flow filter membrane.
3. The method for extracting sheep placenta ultramicrons as claimed in claim 2, wherein the cut-off molecular weight of the tangential flow filter membrane is 4 KD-6 KD.
4. The method for extracting the sheep placenta factor ultramicro as claimed in claim 2, wherein the complex enzyme preparation comprises one or more of trypsin, papain, lipase, neutral protease, bromelain or chymotrypsin.
5. The amniocin ultramicron factor mask solution as claimed in claim 1, wherein the preservative is one or more of methylparaben, propylparaben and chlorphenesin.
6. The sheep placenta factor-containing mask solution according to claim 1, wherein the thickener is one or more of hydroxyethyl cellulose, carbomer, xanthan gum and sodium acetate.
7. The amniocin ultramicro factor mask solution as claimed in claim 1, wherein the preparation method comprises mixing the components in proportion, and stirring at 40-60 ℃ for 3-5h at 600-1000 r/min to obtain the amniocin ultramicro factor mask solution.
8. The amniocin microfactor mask solution of any of claims 1-7, wherein the microfactors are active ingredients secreted by living cells and having a molecular weight of less than 5 KD; the ultramicrofactor can be applied to the preparation of cosmetics related to the ultramicrofactor.
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|---|---|---|---|---|
| CN1333294A (en) * | 2000-07-11 | 2002-01-30 | 张建军 | Novel process for extracting purified sheed fetal agent and method for testing biological activity thereof |
| JP2002179528A (en) * | 2000-12-14 | 2002-06-26 | Japan Natural Laboratory Co Ltd | Peeling cosmetic and peeling soap |
| CN102488713A (en) * | 2011-12-26 | 2012-06-13 | 重庆大学 | Method for preparing sheep placenta extract and sheep placenta hydrolyzed collagen concentrated solution |
| CN109350627A (en) * | 2018-11-28 | 2019-02-19 | 广东冠龙生物科技有限公司 | A kind of freeze-dried sheep placenta extract powder and preparation method thereof |
| CN110478305A (en) * | 2019-08-21 | 2019-11-22 | 银川凤仪堂生物工程有限公司 | A kind of preparation process and its application of sheep placenta extract |
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2021
- 2021-08-02 CN CN202110880272.5A patent/CN113577005A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1333294A (en) * | 2000-07-11 | 2002-01-30 | 张建军 | Novel process for extracting purified sheed fetal agent and method for testing biological activity thereof |
| JP2002179528A (en) * | 2000-12-14 | 2002-06-26 | Japan Natural Laboratory Co Ltd | Peeling cosmetic and peeling soap |
| CN102488713A (en) * | 2011-12-26 | 2012-06-13 | 重庆大学 | Method for preparing sheep placenta extract and sheep placenta hydrolyzed collagen concentrated solution |
| CN109350627A (en) * | 2018-11-28 | 2019-02-19 | 广东冠龙生物科技有限公司 | A kind of freeze-dried sheep placenta extract powder and preparation method thereof |
| CN110478305A (en) * | 2019-08-21 | 2019-11-22 | 银川凤仪堂生物工程有限公司 | A kind of preparation process and its application of sheep placenta extract |
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