CN111643389A

CN111643389A - Anti-wrinkle whitening repair mask containing marine bioactive peptide and preparation method thereof

Info

Publication number: CN111643389A
Application number: CN202010498159.6A
Authority: CN
Inventors: 张雯雯
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-06-04
Filing date: 2020-06-04
Publication date: 2020-09-11

Abstract

The invention provides an anti-wrinkle whitening repair mask containing marine biological active peptide and a preparation method thereof, particularly the marine biological active peptide is used as the source of collagen active peptide, the biological active peptide with different molecular weight gradients is prepared by a specific enzymolysis method, and plant-derived anti-wrinkle and whitening active components are further compounded to prepare the anti-wrinkle whitening repair mask which has good stability, high safety and easy transdermal absorption, and the preparation process is simple, the cost is low, and the anti-wrinkle whitening repair mask is suitable for industrial mass production; and the facial mask has excellent anti-wrinkle and whitening effects.

Description

Anti-wrinkle whitening repair mask containing marine bioactive peptide and preparation method thereof

Technical Field

The invention belongs to the technical field of mask materials, and particularly relates to an anti-wrinkle whitening repair mask containing marine bioactive peptides and a preparation method thereof.

Background

The facial mask is a category of skin care products, is a carrier of the beauty care products, and is applied on the face for beauty functions such as moisturizing, whitening, anti-aging, grease balancing and the like. The facial mask has the effects that the facial mask is covered on the face for a short time, the outside air and pollution are temporarily isolated, the skin temperature is increased, pores of the skin are expanded, the secretion and metabolism of sweat glands are promoted, the oxygen content of the skin is increased, products of metabolism of epidermal cells and accumulated grease substances are favorably removed from the skin, water in the facial mask permeates into the horny layer of the epidermis, the skin becomes soft, and the skin is naturally bright and elastic.

Wherein, the collagen is the main effective component of the mask, and has more content and wider distribution in mammals. However, due to differences in amino acid composition, degree of crosslinking, and the like, collagen derived from marine animals is significantly superior to collagen derived from terrestrial animals in some aspects, such as having low antigenicity, allergic properties, and the like. Therefore, marine animal collagen may gradually replace terrestrial animal collagen.

Because collagen belongs to macromolecular protein and is not easy to permeate into the skin, collagen is often prepared into micromolecular collagen peptide as an active ingredient of the mask in the prior art. Collagen peptide, a bioactive peptide, is a molecular polymer between proteins and amino acids. At present, the collagen is mostly produced by adopting an enzyme method, but the decomposition of the collagen in the prior art is insufficient, and the hydrolysis rate is low.

The existing masks in the market can be divided into moisturizing masks, whitening masks, spot-fading masks, anti-wrinkle masks, cleaning masks and the like according to the effects, and in the prior art, some collagen, plant extracts or chemical components are usually added to achieve the anti-wrinkle whitening effect, so that the effect is not obvious, and the mask does not have long-term effects.

Therefore, the anti-wrinkle whitening repair mask rich in marine bioactive peptides, which has the advantages of good stability, high safety, easy transdermal absorption, low cost, simple preparation process, suitability for industrial mass production, excellent effect and capability of making up the defects of the prior art, is urgently needed in the field.

Disclosure of Invention

In view of the above, the invention aims to provide an anti-wrinkle whitening repair mask containing marine biological active peptides and a preparation method thereof, the marine organisms are used as a source of collagen active peptides, the biological active peptides with different molecular weight gradients are prepared by an enzymolysis method, and plant-derived anti-wrinkle and whitening active components are further compounded to prepare the anti-wrinkle whitening repair mask rich in the marine biological active peptides, which has the advantages of good stability, high safety, easiness in transdermal absorption, low cost, simple preparation process, suitability for industrial mass production, excellent effect and capability of making up the defects of the prior art.

In order to achieve the purpose, the invention provides an anti-wrinkle whitening and repairing facial mask containing marine bioactive peptides, which comprises a facial mask cloth and essence, wherein the essence comprises the following raw materials in parts by weight: 0.1-0.3 part of marine bioactive peptide, 0.2-0.5 part of hyaluronic acid, 0.02-0.04 part of oat glucan, 0.05-0.08 part of ceramide, 0.2-0.4 part of arbutin, 0.1-0.3 part of grape seed extract, 0.02-0.04 part of allantoin, 0.05-0.1 part of humectant, 0.04-0.06 part of emulsifier, 0.05-0.1 part of thickener and 80-100 parts of water.

Preferably, the marine organism active peptide source and one of sturgeon, sea bass, tuna, mackerel and salmon are prepared by the following steps:

(1) cleaning fish skin, decocting in water at 70-80 deg.C for 1-2 hr, and filtering with 300 mesh gauze to obtain collagen water solution;

(2) and (2) adding a hydrolyzed complex enzyme into the collagen aqueous solution in the step (1), and carrying out enzymolysis for 3-4h at 45-55 ℃, wherein the complex enzyme consists of the following raw materials in parts by weight: 10-30 parts of alkaline protease, 8-15 parts of neutral protease, 10-15 parts of papain, 8-10 parts of flavourzyme and 5-8 parts of pepsin;

(3) inactivating enzyme, filtering to obtain enzymolysis solution, adding active carbon into the enzymolysis solution, stirring and mixing for 10-15min, standing for 30-40min, and filtering to remove active carbon to obtain decolorized solution;

(4) and (4) respectively carrying out sectional interception on the decolorized solution in the step (3) by adopting ultrafiltration membrane packages with molecular weight cut-off of 1-5KDa to obtain marine organism active peptides with different molecular weight cut-off.

Preferably, the preparation method of the grape seed extract comprises the following steps: pulverizing grape seed, adding 10-20 weight times of 70% ethanol solution, ultrasonic extracting for 20-40min to obtain grape seed extractive solution, and spray drying to obtain powder.

Preferably, the hyaluronic acid consists of macromolecular hyaluronic acid and small molecular hyaluronic acid according to the weight ratio of 1-2:1, wherein the molecular weight of the macromolecular hyaluronic acid is 100-150KDa, and the molecular weight of the small molecular hyaluronic acid is 60-80 KDa.

Preferably, the humectant is selected from one or more of butanediol, glycerol, trehalose, pentanediol and erythritol.

Preferably, the emulsifier is selected from one or more of cetearyl glucoside, cetearyl alcohol, polysorbate, olive oleate, castor oil polyoxyethylene ether-80, hydrogenated castor oil polyoxyethylene ether-20.

Preferably, the thickener is selected from one or more of xanthan gum, carbomer, guar gum, hydroxyethyl cellulose, glycerol acrylate.

The invention also aims to provide a preparation method of the anti-wrinkle whitening repair mask containing the marine bioactive peptide, which comprises the following steps:

(1) weighing the components in parts by weight, heating to 40-45 ℃, stirring and dispersing uniformly to form essence, and storing in a sterile environment for later use;

(2) spreading and superposing the sterilized mask cloth in a tray, adding the essence liquid obtained in the step (1) for soaking for 10-20 min;

(3) and (3) putting the tray with the soaked mask cloth obtained in the step (2) into a freeze dryer, freezing for 30-50h at-50 ℃ to-28 ℃, and bagging and sealing in a sterile environment after freeze drying to obtain the mask.

The invention has the following beneficial effects:

(1) the method has the advantages that the marine organisms, particularly the fish skin, are selected, the specific complex enzyme is utilized for enzymolysis, and the gradient collagen peptide is prepared; meanwhile, the gradient collagen peptide contains collagen peptides with different molecular weights, so that the collagen peptide has better activity compared with the collagen peptide with single molecular weight, small molecules are easier to permeate into the skin, and large molecules have better moisturizing effect and can have repairing and moisturizing effects;

(2) the essence of the facial mask is rich in functional components such as hyaluronic acid, oat glucan, plant active factors (arbutin and grape seed extract) and the like, has a synergistic effect to achieve the effects of resisting wrinkles and whitening, and particularly has remarkable effects on the whitening effect of arbutin and the anti-wrinkle effect of grape seed extract.

(3) The invention utilizes the freeze-drying technology to solve the problem that the activity of the components of the traditional facial mask essence is easy to destroy, and meanwhile, the freeze-drying can really realize no addition of preservatives and can reduce the irritation caused by the preservatives. The facial mask is convenient to carry and use after being freeze-dried, and can be matched with pure water, lotion, spray, essence and the like according to personal preference and requirements.

Detailed Description

For a further understanding of the invention, reference will now be made to the preferred embodiments of the present invention by way of example, and it is to be understood that the description is intended to further illustrate features and advantages of the present invention and is not intended to limit the scope of the claims which follow. All of the starting materials of the present invention, without particular limitation as to their source, may be purchased commercially or prepared according to conventional methods well known to those skilled in the art.

Example 1

The anti-wrinkle whitening repair mask containing the marine bioactive peptide comprises mask cloth and essence, wherein the essence comprises the following raw materials in parts by weight: 0.3 part of marine bioactive peptide, 0.5 part of hyaluronic acid, 0.04 part of oat glucan, 0.08 part of ceramide, 0.4 part of arbutin, 0.3 part of grape seed extract, 0.04 part of allantoin, 0.1 part of humectant, 0.06 part of emulsifier, 0.1 part of thickener and 100 parts of water;

the preparation method of the marine bioactive peptide comprises the following steps:

(1) washing tuna skin, boiling in water at 80 ℃ for 2h, and filtering with 300-mesh gauze to obtain a collagen aqueous solution;

(2) and (2) applying a hydrolyzed complex enzyme to the collagen aqueous solution in the step (1), and carrying out enzymolysis for 4 hours at 50 ℃, wherein the complex enzyme consists of the following raw materials in parts by weight: 30 parts of alkaline protease, 15 parts of neutral protease, 10 parts of papain, 10 parts of flavourzyme and 5 parts of pepsin;

(3) inactivating enzyme, filtering to obtain enzymolysis solution, adding active carbon into the enzymolysis solution, stirring and mixing for 15min, standing for 40min, and filtering to remove active carbon to obtain decolorized solution;

(4) respectively carrying out segmented interception on the decolorized solution in the step (3) by adopting ultrafiltration membranes with the molecular weight interception of 1-5KDa to obtain marine organism active peptides with different molecular weight interception;

the preparation method of the grape seed extract comprises the following steps: pulverizing grape seed, adding 20 weight times of 70% ethanol solution, and ultrasonic extracting for 40min to obtain grape seed extract;

the hyaluronic acid is prepared from macromolecular hyaluronic acid and micromolecular hyaluronic acid according to the weight ratio of 1:1, the molecular weight of the macromolecular hyaluronic acid is 150KDa, and the molecular weight of the small molecular hyaluronic acid is 60 KDa;

the humectant is prepared from butanediol and glycerol in a weight ratio of 1: 2; the emulsifier is a mixture of cetearyl glucoside and castor oil polyoxyethylene ether-80 in a weight ratio of 1: 1; the thickening agent is xanthan gum;

the preparation method comprises the following steps:

(1) weighing the components in parts by weight, heating to 45 ℃, stirring and dispersing uniformly to form essence, and storing in a sterile environment for later use;

(2) spreading and superposing the sterilized mask cloth in a tray, adding the essence liquid obtained in the step (1) for soaking for 20 min;

(3) and (3) putting the tray with the soaked mask cloth obtained in the step (2) into a freeze dryer, freezing for 50h at-50 ℃, and bagging and sealing in a sterile environment after freeze drying to obtain the mask.

Example 2

The anti-wrinkle whitening repair mask containing the marine bioactive peptide comprises mask cloth and essence, wherein the essence comprises the following raw materials in parts by weight: 0.2 part of marine bioactive peptide, 0.4 part of hyaluronic acid, 0.03 part of oat glucan, 0.05 part of ceramide, 0.2 part of arbutin, 0.2 part of grape seed extract, 0.03 part of allantoin, 0.08 part of humectant, 0.05 part of emulsifier, 0.07 part of thickener and 90 parts of water;

(1) washing sturgeon skin, boiling the sturgeon skin for 1h at 70 ℃, and filtering the sturgeon skin by using 300-mesh gauze to obtain a collagen aqueous solution;

(2) and (2) applying a hydrolyzed complex enzyme to the collagen aqueous solution in the step (1), and carrying out enzymolysis for 4 hours at 50 ℃, wherein the complex enzyme consists of the following raw materials in parts by weight: 20 parts of alkaline protease, 8 parts of neutral protease, 12 parts of papain, 9 parts of flavourzyme and 7 parts of pepsin;

(3) inactivating enzyme, filtering to obtain enzymolysis solution, adding active carbon into the enzymolysis solution, stirring and mixing for 10min, standing for 30min, and filtering to remove active carbon to obtain decolorized solution;

the preparation method of the grape seed extract comprises the following steps: pulverizing grape seed, adding 15 weight times of 70% ethanol solution, and ultrasonic extracting for 20min to obtain grape seed extract;

the hyaluronic acid is prepared from macromolecular hyaluronic acid and small molecular hyaluronic acid according to a weight ratio of 2:1, the molecular weight of the macromolecular hyaluronic acid is 100KDa, and the molecular weight of the small molecular hyaluronic acid is 70 KDa;

the humectant is prepared from trehalose and pentanediol, wherein the weight ratio of the trehalose to the pentanediol is 1: 2; the emulsifier is a mixture of cetearyl glucoside and castor oil polyoxyethylene ether-80 in a weight ratio of 1: 1; the thickening agent is a mixture of hydroxyethyl cellulose and glycerol acrylate in a weight ratio of 2: 1;

the preparation method comprises the following steps:

(1) weighing the components in parts by weight, heating to 40 ℃, stirring and dispersing uniformly to form essence, and storing in a sterile environment for later use;

(2) spreading and superposing the sterilized mask cloth in a tray, adding the essence liquid obtained in the step (1) for soaking for 15 min;

(3) and (3) putting the tray with the soaked mask cloth obtained in the step (2) into a freeze dryer, freezing for 40h at-45 ℃, and bagging and sealing in a sterile environment after freeze drying to obtain the mask.

Example 3

The anti-wrinkle whitening repair mask containing the marine bioactive peptide comprises mask cloth and essence, wherein the essence comprises the following raw materials in parts by weight: 0.2 part of marine bioactive peptide, 0.3 part of hyaluronic acid, 0.03 part of oat glucan, 0.06 part of ceramide, 0.2 part of arbutin, 0.2 part of grape seed extract, 0.02 part of allantoin, 0.06 part of humectant, 0.04 part of emulsifier, 0.06 part of thickener and 80 parts of water;

(1) washing the skin of the sea bass, boiling the skin in water at 75 ℃ for 1h, and filtering the skin by using 300-mesh gauze to obtain a collagen aqueous solution;

(2) and (2) applying a hydrolyzed complex enzyme to the collagen aqueous solution in the step (1), and carrying out enzymolysis for 4 hours at 45 ℃, wherein the complex enzyme consists of the following raw materials in parts by weight: 10 parts of alkaline protease, 15 parts of neutral protease, 15 parts of papain, 10 parts of flavourzyme and 5 parts of pepsin;

the hyaluronic acid is prepared from macromolecular hyaluronic acid and micromolecular hyaluronic acid according to the weight ratio of 1:2, the molecular weight of the macromolecular hyaluronic acid is 120KDa, and the molecular weight of the small molecular hyaluronic acid is 70 KDa;

the humectant is prepared from butanediol and glycerol in a weight ratio of 1: 2; the emulsifier is a mixture of cetearyl glucoside and castor oil polyoxyethylene ether-80 in a weight ratio of 1: 1; the thickening agent is a mixture of carbomer and guar gum in a weight ratio of 1: 2;

the preparation method comprises the following steps:

(3) and (3) putting the tray with the soaked mask cloth obtained in the step (2) into a freeze dryer, freezing for 50h at-30 ℃, and bagging and sealing in a sterile environment after freeze drying to obtain the mask.

Comparative example 1

(3) inactivating enzyme, filtering to obtain enzymolysis solution, adding active carbon into the enzymolysis solution, stirring and mixing for 15min, standing for 340min, and filtering to remove active carbon to obtain decolorized solution;

(4) carrying out segmented interception on the decolorized solution in the step (3) by adopting an ultrafiltration membrane package with the molecular weight cutoff of 3KDa to obtain the marine biological active peptide;

the preparation method comprises the following steps:

Comparative example 2

The anti-wrinkle whitening repair mask containing the marine bioactive peptide comprises mask cloth and essence, wherein the essence comprises the following raw materials in parts by weight: 0.3 part of marine bioactive peptide, 0.5 part of hyaluronic acid, 0.04 part of oat glucan, 0.08 part of ceramide, 0.4 part of arbutin, 0.04 part of allantoin, 0.1 part of humectant, 0.06 part of emulsifier, 0.1 part of thickener and 100 parts of water;

the preparation method comprises the following steps:

Comparative example 3

The anti-wrinkle whitening repair mask containing the marine bioactive peptide comprises mask cloth and essence, wherein the essence comprises the following raw materials in parts by weight: 0.3 part of marine bioactive peptide, 0.5 part of hyaluronic acid, 0.04 part of oat glucan, 0.08 part of ceramide, 0.3 part of grape seed extract, 0.04 part of allantoin, 0.1 part of humectant, 0.06 part of emulsifier, 0.1 part of thickener and 100 parts of water;

the hyaluronic acid consists of macromolecular hyaluronic acid and small molecular hyaluronic acid according to a weight ratio of 11, wherein the molecular weight of the macromolecular hyaluronic acid is 150KDa, and the molecular weight of the small molecular hyaluronic acid is 60 KDa;

the preparation method comprises the following steps:

And (3) anti-wrinkle test: the skin elasticity index R7 value was measured using a skin elasticity meter Cotomermapa 580 from CK, Germany, with a constant negative pressure of 450mbar during the test.

60 tested persons with obvious forehead wrinkles are selected and are all women in the age of 35-45 years, and every 10 persons are randomly divided into 1 group and 6 groups. The forehead of the testee can not use any product within 3 days before the test, the forehead skin is selected as a test area, any 5 points in the test area are selected for measurement, and the average value is obtained and recorded. The first measurement was made before using the sample and recorded as the initial value. After the test is started, the liquid is used once in the morning and evening every day, a proper amount of samples are uniformly smeared around the forehead, the liquid is guaranteed to be smeared on a test area and be fully absorbed, and the liquid is tested once every week after the liquid is used, and is used for four weeks. Specification of indexes: the closer the skin elasticity index R7 is to 1, the better the skin elasticity is. Each set of data was averaged after removing one maximum and one minimum from each test, and the test results are shown in table 1:

TABLE 1

Test set	Initial value	First week	Second week	The third week	The fourth side
						Example 1	0.426	0.504	0.695	0.748	0.814
Example 2	0.432	0.533	0.679	0.768	0.827
						Example 3	0.431	0.546	0.703	0.789	0.831
Comparative example 1	0.417	0.474	0.556	0.625	0.704
						Comparative example 2	0.425	0.496	0.575	0.614	0.734
Comparative example 3	0.419	0.566	0.645	0.705	0.793

The data in table 1 show that the essence can greatly improve the skin elasticity of a subject, and the effect is better than that of a comparative example, and further shows that the skin elasticity can be greatly improved by selecting the gradient collagen peptide and the plant source active factor, so that fine lines and wrinkles are concealed, and the effects of resisting wrinkles and preserving moisture are achieved.

Tyrosinase inhibition experiments: tyrosinase with enzyme activity of 5771U/mg and L-DOPA of 0.5mmol/L were dissolved in NaH2PO4-Na2HPO4 buffer solution with pH of 50mmol/L and 6.8. Adding 0.5g of lyophilized facial mask into 50ml of L-DOPA solution, keeping the temperature in a water bath kettle at 30 ℃ for 10min, adding 1ml of tyrosinase solution, immediately mixing, measuring the absorbance of the reaction solution at 475nm after 2min, and taking the blank without adding materials as a blank control. The results are shown in Table 2:

TABLE 2

	Example 1	Comparative example 3
			Tyrosinase inhibition rate	95％	87％

Among them, it is known from the data of table 2 that the application of arbutin to the mask material can increase the tyrosinase inhibition rate and exert a good whitening effect.

It will be understood by those skilled in the art that the foregoing is only a preferred embodiment of the present invention, and is not intended to limit the invention, and that any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims

1. An anti-wrinkle whitening repair mask containing marine bioactive peptides comprises mask cloth and essence, and is characterized in that: the essence consists of the following raw materials in parts by weight: 0.1-0.3 part of marine bioactive peptide, 0.2-0.5 part of hyaluronic acid, 0.02-0.04 part of oat glucan, 0.05-0.08 part of ceramide, 0.2-0.4 part of arbutin, 0.1-0.3 part of grape seed extract, 0.02-0.04 part of allantoin, 0.05-0.1 part of humectant, 0.04-0.06 part of emulsifier, 0.05-0.1 part of thickener and 80-100 parts of water.

2. The anti-wrinkle whitening repair mask containing marine bioactive peptides as claimed in claim 1, is characterized in that: the marine organism active peptide source is selected from sturgeon, sea bass, tuna, mackerel and salmon.

3. The anti-wrinkle whitening repair mask containing marine bioactive peptides as claimed in claim 2, is characterized in that: the preparation method of the marine bioactive peptide comprises the following steps:

step 1, cleaning fish skin, boiling the fish skin in water at 70-80 ℃ for 1-2h, and filtering the fish skin by using 300-mesh gauze to obtain a collagen aqueous solution;

step 2, adding a hydrolyzed complex enzyme into the collagen aqueous solution obtained in the step 1, and carrying out enzymolysis for 3-4h at 45-55 ℃, wherein the complex enzyme comprises the following raw materials in parts by weight: 10-30 parts of alkaline protease, 8-15 parts of neutral protease, 10-15 parts of papain, 8-10 parts of flavourzyme and 5-8 parts of pepsin;

step 3, inactivating enzyme, filtering to obtain enzymolysis liquid, adding activated carbon into the enzymolysis liquid, stirring and mixing for 10-15min, standing for 30-40min, and filtering to remove activated carbon to obtain decolorized liquid;

and 4, respectively carrying out sectional interception on the decolorized solution in the step 3 by adopting ultrafiltration membranes with the molecular weight cutoff of 1-5KDa to obtain the marine organism active peptides with different molecular weight cutoffs.

4. The anti-wrinkle whitening repair mask containing marine bioactive peptides as claimed in claim 1, is characterized in that: the preparation method of the grape seed extract comprises the following steps: pulverizing grape seed, adding 10-20 weight times of 70% ethanol solution, ultrasonic extracting for 20-40min to obtain grape seed extractive solution, and spray drying to obtain powder.

5. The anti-wrinkle whitening repair mask containing marine bioactive peptides as claimed in claim 1, is characterized in that: the hyaluronic acid consists of macromolecular hyaluronic acid and small molecular hyaluronic acid according to the weight ratio of 1-2:1, wherein the molecular weight of the macromolecular hyaluronic acid is 100-150KDa, and the molecular weight of the small molecular hyaluronic acid is 60-80 KDa.

6. The anti-wrinkle whitening repair mask containing marine bioactive peptides as claimed in claim 1, is characterized in that: the humectant is selected from one or more of butanediol, glycerol, trehalose, pentanediol and erythritol.

7. The anti-wrinkle whitening repair mask containing marine bioactive peptides as claimed in claim 1, is characterized in that: the emulsifier is one or more selected from cetearyl glucoside, cetearyl alcohol, polysorbate, olive oleate, castor oil polyoxyethylene ether-80, and hydrogenated castor oil polyoxyethylene ether-20.

8. The anti-wrinkle whitening repair mask containing marine bioactive peptides as claimed in claim 1, is characterized in that: the thickening agent is one or more of xanthan gum, carbomer, guar gum, hydroxyethyl cellulose and glycerol acrylate.

9. A preparation method for preparing the anti-wrinkle whitening and repairing mask rich in marine bioactive peptides as claimed in any one of claims 1 to 8 comprises the following steps: (1) weighing the components in parts by weight, heating to 40-45 ℃, stirring and dispersing uniformly to form essence, and storing in a sterile environment for later use; (2) spreading and superposing the sterilized mask cloth in a tray, adding the essence liquid obtained in the step (1) for soaking for 10-20 min; (3) and (3) putting the tray with the soaked mask cloth obtained in the step (2) into a freeze dryer, freezing for 30-50h at-50 ℃ to-28 ℃, and bagging and sealing in a sterile environment after freeze drying to obtain the mask.