CN113456561A - Preparation method and application of essence containing polypeptide and plant extract - Google Patents
Preparation method and application of essence containing polypeptide and plant extract Download PDFInfo
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- CN113456561A CN113456561A CN202110780663.XA CN202110780663A CN113456561A CN 113456561 A CN113456561 A CN 113456561A CN 202110780663 A CN202110780663 A CN 202110780663A CN 113456561 A CN113456561 A CN 113456561A
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 69
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 66
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 64
- 239000000419 plant extract Substances 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 67
- 239000000843 powder Substances 0.000 claims abstract description 37
- 238000000605 extraction Methods 0.000 claims abstract description 24
- 238000004140 cleaning Methods 0.000 claims abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 238000002156 mixing Methods 0.000 claims abstract description 17
- 239000003205 fragrance Substances 0.000 claims abstract description 16
- 238000005507 spraying Methods 0.000 claims abstract description 15
- 239000012528 membrane Substances 0.000 claims abstract description 13
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 13
- 238000004108 freeze drying Methods 0.000 claims abstract description 12
- 102000004190 Enzymes Human genes 0.000 claims abstract description 11
- 108090000790 Enzymes Proteins 0.000 claims abstract description 11
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 11
- 239000000284 extract Substances 0.000 claims abstract description 11
- 239000000341 volatile oil Substances 0.000 claims abstract description 11
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 238000011033 desalting Methods 0.000 claims abstract description 6
- 238000003825 pressing Methods 0.000 claims abstract description 6
- 239000002002 slurry Substances 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims abstract 11
- 241000196324 Embryophyta Species 0.000 claims description 38
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
- 229940088598 enzyme Drugs 0.000 claims description 10
- 210000004761 scalp Anatomy 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 240000003915 Lophatherum gracile Species 0.000 claims description 7
- 241000237502 Ostreidae Species 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 235000020636 oyster Nutrition 0.000 claims description 6
- 241001116389 Aloe Species 0.000 claims description 5
- 241000205585 Aquilegia canadensis Species 0.000 claims description 5
- 102000029816 Collagenase Human genes 0.000 claims description 5
- 108060005980 Collagenase Proteins 0.000 claims description 5
- 241000899950 Salix glauca Species 0.000 claims description 5
- 235000011399 aloe vera Nutrition 0.000 claims description 5
- 229960002424 collagenase Drugs 0.000 claims description 5
- 235000013372 meat Nutrition 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- 239000012299 nitrogen atmosphere Substances 0.000 claims description 5
- 235000010254 Jasminum officinale Nutrition 0.000 claims description 2
- 240000005385 Jasminum sambac Species 0.000 claims description 2
- 240000002853 Nelumbo nucifera Species 0.000 claims description 2
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims description 2
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims description 2
- 241000220317 Rosa Species 0.000 claims description 2
- 108090000787 Subtilisin Proteins 0.000 claims description 2
- 239000000463 material Substances 0.000 claims 2
- 238000001816 cooling Methods 0.000 claims 1
- 238000007710 freezing Methods 0.000 claims 1
- 230000008014 freezing Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 15
- 210000003491 skin Anatomy 0.000 abstract description 13
- 230000009286 beneficial effect Effects 0.000 abstract description 4
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- 231100000274 skin absorption Toxicity 0.000 abstract description 3
- 239000000047 product Substances 0.000 description 8
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- 150000001875 compounds Chemical class 0.000 description 5
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- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 4
- 230000006872 improvement Effects 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- -1 phenolic acid compounds Chemical class 0.000 description 4
- 239000007921 spray Substances 0.000 description 4
- 244000063299 Bacillus subtilis Species 0.000 description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 238000000889 atomisation Methods 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 208000002874 Acne Vulgaris Diseases 0.000 description 2
- NGFMICBWJRZIBI-JZRPKSSGSA-N Salicin Natural products O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O1)c1c(CO)cccc1 NGFMICBWJRZIBI-JZRPKSSGSA-N 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 206010000496 acne Diseases 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- NGFMICBWJRZIBI-UHFFFAOYSA-N alpha-salicin Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UHFFFAOYSA-N 0.000 description 2
- 230000003110 anti-inflammatory effect Effects 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 210000004209 hair Anatomy 0.000 description 2
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- 230000004048 modification Effects 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- NGFMICBWJRZIBI-UJPOAAIJSA-N salicin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UJPOAAIJSA-N 0.000 description 2
- 229940120668 salicin Drugs 0.000 description 2
- 229940084038 salix alba bark extract Drugs 0.000 description 2
- 102000001381 Arachidonate 5-Lipoxygenase Human genes 0.000 description 1
- 108010093579 Arachidonate 5-lipoxygenase Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 235000011201 Ginkgo Nutrition 0.000 description 1
- 244000194101 Ginkgo biloba Species 0.000 description 1
- 235000008100 Ginkgo biloba Nutrition 0.000 description 1
- 241000192132 Leuconostoc Species 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 102000004005 Prostaglandin-endoperoxide synthases Human genes 0.000 description 1
- 108090000459 Prostaglandin-endoperoxide synthases Proteins 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Natural products CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 description 1
- 230000001032 anti-candidal effect Effects 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N benzo-alpha-pyrone Natural products C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 229960000956 coumarin Drugs 0.000 description 1
- 235000001671 coumarin Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 229930182486 flavonoid glycoside Natural products 0.000 description 1
- 150000007955 flavonoid glycosides Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 230000036074 healthy skin Effects 0.000 description 1
- 238000007602 hot air drying Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 244000005714 skin microbiome Species 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9755—Gymnosperms [Coniferophyta]
- A61K8/9761—Cupressaceae [Cypress family], e.g. juniper or cypress
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method of essence containing polypeptide and plant extract, which comprises the following steps: cleaning polypeptide raw materials, crushing into slurry for later use, cleaning plant raw materials, freeze-drying, and rapidly adding into a wall breaking machine for crushing into powder for later use; placing the polypeptide raw material powder in an enzymolysis tank, adding a complex enzyme for enzymolysis, filtering by using an ultrafiltration membrane, and desalting and concentrating to obtain a small molecular polypeptide concentrated solution; putting the powder of the plant raw material into an ultrafiltration membrane for wrapping, pressing and fixing the powder into a flat lamellar shape, spraying the extraction liquid on two sides at high pressure to wash the powder of the plant raw material for extraction, and cleaning and purifying to obtain the extraction liquid; mixing the small molecular polypeptide concentrated solution and the extract liquor according to a proportion, and adding a fragrance agent to obtain a finished product of the essence. The essential oil prepared by the method disclosed by the invention contains small molecular peptides, is beneficial to skin absorption, can improve and enhance cell activity, has the effect of repairing hair follicles, and can repair and improve skin by matching with other plant extract.
Description
Technical Field
The invention relates to the technical field of production of daily chemical articles, and particularly relates to a preparation method and application of essence containing polypeptide and plant extract.
Background
With the continuous improvement of living standard and living quality of people, the requirements of consumers on beauty and skin care products necessary for daily life are higher and higher, and the influence of the idea of 'returning to nature' tending to plant skin care is emphasized more and more, and in recent years, the scalp health problem is more and more prominent due to factors such as accelerated living rhythm, night stay, irregular diet, hair scalding and dyeing, environmental pollution and the like of people. Data from the scalp health research center of the Chinese health education Association show that the scalp of more than 60% of adults in our country have health problems of varying degrees. However, human perception of scalp care is still at a low level and there are still few scalp and facial care products on the market.
The healthy skin ecological environment is maintained by three major balances: oil, flora and metabolic balance. If the three major balance environments are unbalanced, the skin is easy to produce oil and become greasy, a large amount of harmful bacteria are bred, and phenomena of itching, acne growing and the like occur. In addition, the face and scalp are relatively thin skin in the human body, and age 3-10 times faster than other locations. The scalp is dry, easy to age, inflame, face oiliness, acne and speckle and the like due to wind blowing, sun drying, dyeing, scalding, hot air drying and the like, and the essential oil has small molecular weight, is easy to permeate into the skin to be absorbed and enter the deep layer of the skin, and has a very good effect of stimulating the activity of the skin, so the essential oil hair care product and the essential oil skin care product are gradually favored by people. The existing essential oils on the market are insufficient in oil removal and cleaning capabilities, still have insufficient absorption effect and poor in persistence, so that an essential oil capable of solving the problems is required to be found.
Disclosure of Invention
The invention aims to: aiming at the problems mentioned in the background technology, the essence prepared by the invention contains small molecular peptides which are small molecular amino acids and are beneficial to skin absorption, and meanwhile, the peptide substances can improve and enhance the cell activity and have the effect of repairing hair follicles, and can repair and improve the skin by being matched with other plant extract.
In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows:
a preparation method of essence containing polypeptide and plant extract comprises the following steps:
(1) pretreatment: cleaning polypeptide raw materials, crushing into slurry for later use, cleaning plant raw materials, freeze-drying, and rapidly adding into a wall breaking machine for crushing into powder for later use;
(2) polypeptide extraction: placing the polypeptide raw material powder in an enzymolysis tank, adding a complex enzyme for enzymolysis, filtering by using an ultrafiltration membrane, and desalting and concentrating to obtain a small molecular polypeptide concentrated solution;
(3) extracting plant extract: putting the powder of the plant raw material into an ultrafiltration membrane for wrapping, pressing and fixing the powder into a flat lamellar shape, spraying the extraction liquid on two sides at high pressure to wash the powder of the plant raw material for extraction, and cleaning and purifying to obtain the extraction liquid;
(4) mixing and adjusting: mixing the small molecular polypeptide concentrated solution and the extract liquor according to a proportion, and adding a fragrance agent to obtain a finished product of the essence.
Further, the polypeptide raw material is oyster meat, and when the polypeptide raw material is cleaned, the polypeptide raw material is firstly placed in clear water to be soaked for 3-5 hours, and then the mud and sand in the polypeptide raw material are cut open and cleaned.
Further, the plant raw materials comprise the following raw materials in parts by weight: 15-35 parts of white willow bark, 35-50 parts of lophatherum gracile, 12-18 parts of cacumen biotae, 15-25 parts of honeysuckle and 10-15 parts of aloe.
Further, the freeze drying is to place the plant raw materials in a closed container in a vacuum environment, introduce liquid nitrogen into the closed container to cool and freeze, control an air outlet and an air inlet to keep the closed container in a nitrogen atmosphere, then close the air inlet and the air outlet, vacuumize to keep the closed container in a certain pressure range, and open a microwave generator in the closed container to dry when the closed container is kept at the ambient temperature.
Further, the vacuum degree of the closed container after vacuum pumping is 20-30Pa, the power of the microwave generator is 250-300w/kg, and the drying time is 1.5-2 hours.
Further, in the step (2), the compound enzyme is prepared from collagenase and subtilisin mesoscale protease in a ratio of (1-5): (2-3) mixing.
Further, in the step (3), the high-pressure spraying is to spray the plant raw material powder by using an atomizing nozzle.
Further, in the step (4), the fragrance agent is fragrance essential oil extracted from one or more of jasmine, rose and lotus.
The white willow bark extract mainly contains salicin, and the salicin has good anti-inflammatory effect due to the aspirin-like property, so that the white willow bark extract has good effects of balancing skin flora and improving excessive grease.
The folium Bambusae contains abundant flavonoids, bioactive polysaccharides and other effective components, such as phenolic acid compounds, anthraquinone compounds, terpene lactone, special amino acids, active skin, and microelements such as manganese, zinc, and selenium. The functional factors contained in the lophatherum gracile leaves are mainly flavonoid glycoside and coumarin lactone, the content and the biological activity of the active ingredients are both comparable to those of the ginkgo leaves, and the bamboo leaf extract has excellent functions of resisting free radicals, oxidation and aging.
The cacumen biotae plays an anti-inflammatory role by inhibiting the activity of 5-lipoxygenase and the components of cyclooxygenase, the cacumen biotae water decoction has an inhibiting effect on staphylococcus aureus, the alcohol extract of the cacumen biotae water decoction has a stronger inhibiting effect on staphylococcus aureus than the water decoction, the cacumen biotae extract also has an inhibiting effect on beta coccus, and the cacumen biotae extract contains phenolic substances and has an anti-candida activity effect.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. the essence prepared by the invention contains micromolecular polypeptide, the peptide is micromolecular amino acid, the skin absorption is facilitated, meanwhile, the peptide substance can improve and enhance the cell activity and has the hair follicle repairing effect, and the skin can be repaired and improved by matching with other plant extracting solutions.
2. The essence prepared by the invention contains plant extracts, and the lophatherum gracile extract in the plant extracts has good effects of resisting free radicals, aging and oxidation, and can prevent skin aging;
3. according to the preparation method, when the polypeptide is extracted, the ultrafiltration membrane is adopted for direct filtration to obtain the small molecular polypeptide, the operation is simple, and the polypeptide in the oysters can be fully extracted;
4. the plant raw material is processed in a freeze drying mode, so that the plant raw material is processed on the premise of keeping substances in the plant raw material from being oxidized, and the activity of functional factors of the plant raw material is ensured;
5. the plant powder is prepared into a flat shape and sprayed on two sides, so that the plant powder can be permeated and fully extracted by the extraction liquid.
6. The essence prepared by the invention is applied to the scalp and the face, and is beneficial to the absorption of the essence and the repair and improvement of the skin of the head and the face.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail below with reference to preferred embodiments. It should be noted, however, that the numerous details set forth in the description are merely for the purpose of providing the reader with a thorough understanding of one or more aspects of the present invention, which may be practiced without these specific details.
Example 1
A preparation method of essence containing polypeptide and plant extract comprises the following steps:
(1) pretreatment: cleaning polypeptide raw materials, crushing into slurry for later use, cleaning plant raw materials, freeze-drying, and rapidly adding into a wall breaking machine for crushing into powder for later use;
(2) polypeptide extraction: placing polypeptide raw material powder into an enzymolysis tank, adding a compound enzyme for enzymolysis, filtering by using an ultrafiltration membrane, desalting and concentrating to obtain a small molecular polypeptide concentrated solution, wherein the compound enzyme is prepared by mixing collagenase and bacillus subtilis medium protease in a proportion of 1: 2, mixing;
(3) extracting plant extract: putting the powder of the plant raw material into an ultrafiltration membrane for wrapping, pressing and fixing the powder into a flat lamellar shape, spraying the extraction liquid on two sides at high pressure to wash the powder of the plant raw material for extraction, cleaning and purifying to obtain the extraction liquid, wherein the high-pressure spraying is to spray the powder of the plant raw material by adopting an atomization nozzle, and the spraying pressure is 3 MPa;
(4) mixing and adjusting: mixing the small molecular polypeptide concentrated solution and the extract liquor according to a proportion, and adding a fragrance agent to obtain a finished product of the essence.
The polypeptide raw material is oyster meat, and when the polypeptide raw material is cleaned, the polypeptide raw material is firstly placed in clear water to be soaked for 3 hours, and then the interior silt is cut open and cleaned.
The plant raw materials comprise the following raw materials in parts by weight: 15 parts of white willow bark, 35 parts of lophatherum gracile, 12 parts of cacumen biotae, 15 parts of honeysuckle and 10 parts of aloe; the freeze drying is that the plant raw material is placed in a closed container in a vacuum environment, liquid nitrogen is introduced into the closed container to cool and freeze, the air outlet and the air inlet are controlled to keep the closed container in a nitrogen atmosphere, then the air inlet and the air outlet are closed, the closed container is vacuumized to keep the closed container in a certain pressure range, when the closed container is kept at the ambient temperature, a microwave generator in the closed container is started to dry, the vacuum degree of the closed container after vacuumizing is 20Pa, the power of the microwave generator is 250w/kg, and the drying time is 1.5 hours.
The fragrance agent is fragrance essential oil extracted from flos Jasmini sambac.
Example 2
A preparation method of essence containing polypeptide and plant extract comprises the following steps:
(1) pretreatment: cleaning polypeptide raw materials, crushing into slurry for later use, cleaning plant raw materials, freeze-drying, and rapidly adding into a wall breaking machine for crushing into powder for later use;
(2) polypeptide extraction: placing the polypeptide raw material powder into an enzymolysis tank, adding a compound enzyme for enzymolysis, filtering by using an ultrafiltration membrane, desalting and concentrating to obtain a small molecular polypeptide concentrated solution, wherein the compound enzyme is prepared from collagenase and bacillus subtilis medium protease in a ratio of 5: 3, mixing in proportion;
(3) extracting plant extract: putting the powder of the plant raw material into an ultrafiltration membrane for wrapping, pressing and fixing the powder into a flat lamellar shape, spraying the extraction liquid on two sides at high pressure to wash the powder of the plant raw material for extraction, cleaning and purifying to obtain the extraction liquid, wherein the high-pressure spraying is to spray the powder of the plant raw material by adopting an atomization nozzle, and the spraying pressure is 5 MPa;
(4) mixing and adjusting: mixing the small molecular polypeptide concentrated solution and the extract liquor according to a proportion, and adding a fragrance agent to obtain a finished product of the essence.
The polypeptide raw material is oyster meat, and when the polypeptide raw material is cleaned, the polypeptide raw material is firstly placed in clear water to be soaked for 5 hours, and then the interior silt is cut open and cleaned.
The plant raw materials comprise the following raw materials in parts by weight: 35 parts of white willow bark, 50 parts of lophatherum gracile, 18 parts of cacumen biotae, 25 parts of honeysuckle and 15 parts of aloe; the freeze drying is that the plant raw material is placed in a closed container in a vacuum environment, liquid nitrogen is introduced into the closed container to cool and freeze, the air outlet and the air inlet are controlled to keep the closed container in a nitrogen atmosphere, then the air inlet and the air outlet are closed, the closed container is vacuumized to keep the closed container in a certain pressure range, when the closed container is kept at the ambient temperature, a microwave generator in the closed container is started to dry, the vacuum degree of the closed container after vacuumizing is 30Pa, the power of the microwave generator is 300w/kg, and the drying time is 2 hours.
The fragrance agent is fragrance essential oil extracted from flos Rosae Rugosae.
Example 3
A preparation method of essence containing polypeptide and plant extract comprises the following steps:
(1) pretreatment: cleaning polypeptide raw materials, crushing into slurry for later use, cleaning plant raw materials, freeze-drying, and rapidly adding into a wall breaking machine for crushing into powder for later use;
(2) polypeptide extraction: placing the polypeptide raw material powder in an enzymolysis tank, adding a complex enzyme for enzymolysis, filtering by using an ultrafiltration membrane, desalting and concentrating to obtain a small molecular polypeptide concentrated solution, wherein the complex enzyme is prepared by mixing collagenase and bacillus subtilis mesoscale protease in a ratio of 1: 1;
(3) extracting plant extract: putting the powder of the plant raw material into an ultrafiltration membrane for wrapping, pressing and fixing the powder into a flat lamellar shape, spraying the extraction liquid on two sides at high pressure to wash the powder of the plant raw material for extraction, cleaning and purifying to obtain the extraction liquid, wherein the high-pressure spraying is to spray the powder of the plant raw material by adopting an atomization nozzle, and the spraying pressure is 4 MPa;
(4) mixing and adjusting: mixing the small molecular polypeptide concentrated solution and the extract liquor according to a proportion, and adding a fragrance agent to obtain a finished product of the essence.
The polypeptide raw material is oyster meat, and when the polypeptide raw material is cleaned, the polypeptide raw material is firstly placed in clear water to be soaked for 4 hours, and then the interior silt is cut open and cleaned.
The plant raw materials comprise the following raw materials in parts by weight: 25 parts of white willow bark, 40 parts of lophatherum gracile, 15 parts of cacumen biotae, 20 parts of honeysuckle and 12 parts of aloe; the freeze drying is that the plant raw material is placed in a closed container in a vacuum environment, liquid nitrogen is introduced into the closed container to cool and freeze, the air outlet and the air inlet are controlled to keep the closed container in a nitrogen atmosphere, then the air inlet and the air outlet are closed, the closed container is vacuumized to keep the closed container in a certain pressure range, when the closed container is kept at the ambient temperature, a microwave generator in the closed container is started to dry, the vacuum degree of the closed container after vacuumizing is 25Pa, the power of the microwave generator is 280w/kg, and the drying time is 1.8 hours.
The fragrance agent is fragrance essential oil extracted from one or more of flos Jasmini sambac, flos Rosae Rugosae, and flos Nelumbinis.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.
Claims (9)
1. A preparation method of essence containing polypeptide and plant extract is characterized by comprising the following steps:
(1) pretreatment: cleaning polypeptide raw materials, crushing into slurry for later use, cleaning plant raw materials, freeze-drying, and rapidly adding into a wall breaking machine for crushing into powder for later use;
(2) polypeptide extraction: placing the polypeptide raw material powder in an enzymolysis tank, adding a complex enzyme for enzymolysis, filtering by using an ultrafiltration membrane, and desalting and concentrating to obtain a small molecular polypeptide concentrated solution;
(3) extracting plant extract: putting the powder of the plant raw material into an ultrafiltration membrane for wrapping, pressing and fixing the powder into a flat lamellar shape, spraying the extraction liquid on two sides at high pressure to wash the powder of the plant raw material for extraction, and cleaning and purifying to obtain the extraction liquid;
(4) mixing and adjusting: mixing the small molecular polypeptide concentrated solution and the extract liquor according to a proportion, and adding a fragrance agent to obtain a finished product of the essence.
2. The method for preparing the essence containing the polypeptide and the plant extract according to claim 1, wherein the polypeptide raw material is oyster meat, and the polypeptide raw material is firstly soaked in clear water for 3-5 hours and then cut open to clean internal silt when being cleaned.
3. The method for preparing the essence containing the polypeptide and the plant extract according to claim 1, wherein the plant raw materials comprise the following raw materials in parts by weight: 15-35 parts of white willow bark, 35-50 parts of lophatherum gracile, 12-18 parts of cacumen biotae, 15-25 parts of honeysuckle and 10-15 parts of aloe.
4. The method for preparing essence containing polypeptide and plant extract according to claim 3, wherein the freeze-drying is performed by placing the plant material in a closed container under vacuum, introducing liquid nitrogen into the closed container, cooling and freezing, controlling the air inlet and the air outlet to keep the closed container in nitrogen atmosphere, closing the air inlet and the air outlet, vacuumizing to keep the closed container in a certain pressure range, and starting a microwave generator in the closed container to dry when the closed container is kept at ambient temperature.
5. The method as claimed in claim 4, wherein the vacuum degree of the vacuum-sealed container is 20-30Pa, the power of the microwave generator is 250-300w/kg, and the drying time is 1.5-2 hours.
6. The method for preparing essence containing polypeptide and plant extract according to claim 1, wherein in step (2), the complex enzyme is prepared from collagenase and subtilisin mesoproteinase in a ratio of (1-5): (2-3) mixing.
7. The method for preparing essence containing polypeptide and plant extract according to claim 1, wherein in step (3), the high pressure spraying is performed by spraying powder of plant material with an atomizing nozzle.
8. The method for preparing essence containing polypeptide and plant extract according to claim 6, wherein the method comprises the following steps: in the step (4), the fragrance agent is fragrance essential oil extracted from one or more of jasmine, rose and lotus.
9. The method for preparing essence containing polypeptide and plant extract according to claims 1-8, wherein the method comprises the following steps: the prepared essence can be used for caring scalp and face.
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