KR102524240B1 - Skin whitening functional composition comprising fennel extract and trenella fuciformis extract - Google Patents

Abstract

The present invention relates to a functional composition for skin whitening comprising a Foeniculum vulgare extract and a Tremella fuciformis extract as active ingredients, wherein the functional composition for skin whitening according to the present invention contains natural extracts as active ingredients to be non-cytotoxic, and contains the Foeniculum vulgare extract and the Tremella fuciformis extract as the essential active ingredients to significantly increase wrinkle alleviating, whitening, and antioxidant effects as a synergistic effect, thereby being useful as health functional food or cosmetic ingredients for preventing skin aging and for beauty purposes.

Description

Functional composition for skin whitening containing fennel extract and white throat mushroom extract

The present invention relates to a functional composition for skin whitening comprising a fennel extract and a white throat mushroom extract as active ingredients and a manufacturing method thereof.

In general, a functional composition for skin whitening aims to clean the body, make oneself beautiful and attractive, and prevent aging by protecting skin or hair from ultraviolet rays, dryness, pollutants, and the like.

On the other hand, with the recent development of industrial society and the increase in social activities, self-consciousness to actively express oneself has increased, taking health functional foods with skin improvement effects or using cosmetics to care for one's own skin and keep it healthy. The desire to do so is greatly increasing.

However, due to causes such as environmental changes or lifestyle changes, environmental pollution caused by various stresses and pollutants generated in social life, frequent face washing due to makeup habits, and natural skin aging due to age increase, the skin becomes dry and the surface As the skin becomes rough and the skin loses its luster, problems such as wrinkle improvement, whitening, and anti-oxidation are becoming more and more important.

An object of the present invention is to provide a functional composition for skin whitening comprising a fennel extract and a white throat mushroom extract as active ingredients.

An object of the present invention is to provide a functional composition for skin whitening further comprising centella asiatica extract as an active ingredient.

The objects of the present invention are not limited to the above-mentioned objects, and other objects and advantages of the present invention not mentioned above can be understood by the following description and will be more clearly understood by the examples of the present invention. It will also be readily apparent that the objects and advantages of the present invention may be realized by means of the instrumentalities and combinations indicated in the claims.

In order to solve the above problems, the present invention

Preparing a first fermentation broth;

Fermenting fennel and white throat mushrooms with the first fermentation broth;

Adding the fermented fennel and white throat mushroom to an extraction solvent having a pH of 6 to 8;

Preparing preliminary fennel and white throat mushroom extracts by irradiating ultrasonic waves at 20 to 50 kHz and 25 to 40 ° C. for 0.5 to 1.5 hours to the extraction solvent to which the fermented fennel and white throat mushrooms are added; and

preparing fennel and white throat mushroom extracts by filtering the preliminary fennel and white throat mushroom extracts and then concentrating the filtered preliminary fennel and white throat mushroom extracts under reduced pressure; It provides a method for producing a fennel extract and a white throat mushroom extract comprising a.

On the other hand, the present invention provides a functional composition for skin whitening comprising the fennel extract and the white throat mushroom extract as active ingredients.

In one embodiment of the present invention, the fennel extract and white throat mushroom extract may be included in the functional composition for skin whitening in a weight ratio of 5:1 to 1:5, respectively.

In one embodiment of the present invention, the functional composition for skin whitening may further include a Centella Asiatica extract.

In one embodiment of the present invention, the Centella asiatica extract may be included in the functional composition for skin whitening in an amount of 20 to 70% by weight based on the total weight of the fennel extract and the white throat mushroom extract.

The solution to the above problems does not enumerate all the features of the present invention. Various features of the present invention and the advantages and effects thereof will be understood in more detail with reference to the following specific examples.

The functional composition for skin whitening according to the present invention not only has no cytotoxicity by including a natural extract as an active ingredient, but also significantly increases wrinkle improvement, whitening and antioxidant effects by including fennel extract and white throat mushroom extract as essential active ingredients. show effect. Therefore, the functional composition for skin whitening according to the present invention can be usefully used as a cosmetic health functional food or cosmetic for preventing skin aging.

In addition to the above effects, specific effects of the present invention will be described together while explaining specific details for carrying out the present invention.

In the following, embodiments are described in detail. However, since various changes can be made to the embodiments, the scope of the patent application is not limited or limited by these embodiments. It should be understood that all changes, equivalents or substitutes to the embodiments are included within the scope of rights.

Terms used in the examples are used only for descriptive purposes and should not be construed as limiting. Singular expressions include plural expressions unless the context clearly dictates otherwise. In the present invention, terms such as "comprise" or "having" are intended to designate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by a person of ordinary skill in the art to which the embodiment belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related art, and unless explicitly defined in the present application, they should not be interpreted in an ideal or excessively formal meaning. don't

In addition, in the description with reference to the accompanying drawings, the same reference numerals are assigned to the same components regardless of reference numerals, and overlapping descriptions thereof will be omitted. In describing the embodiment, if it is determined that a detailed description of a related known technology may unnecessarily obscure the gist of the embodiment, the detailed description will be omitted.

In addition, in describing the components of the embodiment, terms such as first, second, A, B, (a), (b) may be used. These terms are only used to distinguish the component from other components, and the nature, sequence, or order of the corresponding component is not limited by the term. When an element is described as being “connected”, “coupled” or “connected” to another element, the element may be directly connected or connected to the other element, but there may be another element between the elements. It should be understood that may be "connected", "coupled" or "connected".

Components included in one embodiment and components including common functions will be described using the same names in other embodiments. Unless stated to the contrary, descriptions described in one embodiment may be applied to other embodiments, and detailed descriptions will be omitted to the extent of overlap.

The present invention provides a functional composition for skin whitening comprising fennel extract and white throat mushroom extract as active ingredients.

In the present invention, the 'fennel' belongs to Apiaceae, and is a dried seed, leaf, stem, or fruit of fennel ( Foeniculum vulgare ).

In the present invention, the 'white tree mushroom' ( Tremella fuciformis ) is a plant belonging to the white tree family, and its origin is China, Japan, Korea, etc., and occurs on rotten trees of broad-leaved trees in hilly areas and mountainous areas. White-throated mushrooms are known to have a very good function as a moisturizer because they contain minerals such as calcium and iron and contain water up to 500 times their own weight.

In one embodiment of the present invention, the fennel extract and white throat mushroom extract may be included in a weight ratio of 5: 1 to 1: 5, respectively, and preferably included in a weight ratio of 5: 1 to 1: 1 It may be, and more preferably, it may be included in a weight ratio of 3: 1 to 1: 1.

Specifically, according to Experimental Examples 2 to 4 of the present invention, it was confirmed that the wrinkle improvement, whitening and antioxidant effects were the most excellent in the composition consisting of the fennel extract and the white throat mushroom extract in a ratio of 3:1.

In one embodiment of the present invention, the functional composition for skin whitening of the present invention may further include a Centella Asiatica extract.

According to Experimental Examples 2 to 4 of the present invention, when the centella asiatica extract was additionally included in the sample consisting of the fennel extract and the white throat mushroom extract, it was confirmed that the wrinkle improvement, whitening and antioxidant effects were excellent. Centella asiatica extract may be included in an amount of 20 to 70% by weight based on the total weight of the fennel extract and the white throat mushroom extract.

The functional composition for skin whitening of the present invention includes fennel extract, white throat mushroom extract, centella asiatica extract, and other components commonly used in functional compositions for skin whitening, such as antioxidants, stabilizers, solvents, vitamins, and pigments. and conventional adjuvants such as perfumes, and carriers.

Meanwhile, in the present invention, 'extract' means a substance dissolved in a solvent to separate its active ingredient or characteristic ingredient. Specifically, it includes an extract extracted by adding an extraction solvent to a plant and a fraction obtained by adding a fractionation solvent to an extract prepared by extracting with an extraction solvent. For example, the fennel extract of the present invention is meant to include both an extract extracted by adding a solvent to fennel and a fraction obtained by adding a fractionation solvent thereto.

In one embodiment of the present invention, the present invention provides a health functional food composition comprising the functional composition for skin whitening of the present invention.

In one embodiment of the present invention, the functional composition for skin whitening of the present invention can be used for preparing a pharmaceutical formulation.

According to Experimental Example 1 of the present invention, it was confirmed that the active ingredient of the functional composition for skin whitening and the combination thereof of the present invention have no cytotoxicity, and thus can be used as a component of a health functional food or pharmaceutical formulation.

In one embodiment of the present invention, the health functional food composition containing the functional composition for skin whitening of the present invention may exhibit wrinkle improvement, whitening, and antioxidant effects.

In one embodiment of the present invention, it is possible to provide a cosmetic composition comprising the functional composition for skin whitening of the present invention. The cosmetic composition may exhibit wrinkle improvement, whitening, and antioxidant effects.

The cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , It may be formulated as an oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it may be prepared in the form of a nourishing cream, astringent lotion, softening lotion, lotion, essence, nutritional gel or massage cream.

On the other hand, the present invention provides a method for preparing fennel extract, white throat mushroom, centella asiatica extract. It is described in detail below.

(S1) first fermentation broth preparation step

The method for preparing the fennel extract and white throat mushroom extract according to the present invention may include (S1) preparing a first fermentation broth. This step is a step of preparing a first fermentation broth for fermenting fennel and white throat mushrooms with lactic acid bacteria, and the first fermentation broth is a process of preparing a fermentation broth containing lactic acid bacteria.

At this time, the lactic acid bacteria may be Lactobacillus bacteria, Bifidobacterium bacteria or Streptococcus bacteria. Specifically, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus bulgaricus, Bifillae Bifidobacterium lactis, Bifidobacterium bifidum, Bifidobacterium adolescentis, Streptococcus faecalis, Streptococcus lactis And lactic acid bacteria selected from the group consisting of Streptococcus thermophilus may be used.

In particular, it is preferable to use a complex lactic acid bacteria composed of one or more types of various lactic acid bacteria, and it is preferable to include Lactobacillus acidophilus and Lactobacillus plantarum. The most preferred lactic acid bacteria are complex lactic acid bacteria in which Lactobacillus acidophilus and Lactobacillus plantarum are mixed in a ratio of 1:1 (w/w).

(S2) fermenting fennel and white throat mushrooms with the first fermentation broth

The method for preparing the fennel extract and white throat mushroom extract according to the present invention may include (S2) fermenting fennel and white throat mushroom, respectively, with the first fermentation broth.

At this time, the fermented fennel and white throat mushrooms may be obtained by coarsely pulverizing and finely pulverizing raw fennel and white throat mushrooms using a grinder, and then heat treatment.

As for the conditions for fermenting fennel and white throat mushrooms using the first fermentation broth, the optimal culture temperature and period may be changed depending on the state of fennel and white throat mushrooms used, but 0.01 to 2% (based on the weight of each of fennel and white throat mushrooms) w / w) and fermented for 20 to 24 hours at 30 to 50 degrees, preferably 35 to 45 degrees.

If the step of fermenting the fennel and white throat mushrooms is out of the fermentation time and temperature range of the above numerical range, the fennel and white throat mushrooms may not be sufficiently matured or fermented excessively, resulting in poor marketability.

At this time, it is preferable to culture by adding 0.1 to 0.2% by weight of salt relative to the weight of fennel and white throat mushrooms in order to maintain an appropriate osmotic pressure during fermentation of fennel and white mushrooms.

On the other hand, the step (S2) can be applied to centella asiatica to prepare a centella asiatica extract. It is possible to prepare a centella asiatica extract to be described later by fermenting centella asiatica with a first fermented liquid, and to prepare a functional composition for skin whitening further comprising a centella asiatica extract from the viewpoint of adding effects such as anti-aging, anti-atopic, and anti-acne effects. there is.

Further, according to the present invention, a step (S21) of further fermenting the fermented fennel and white throat mushroom with a second fermentation broth may be further included.

At this time, the additional fermentation step (S21) is preferably carried out after 24 to 72 hours from the viewpoint of stabilizing the fennel and white throat mushrooms that have undergone step (S2), and from the viewpoint of the function of the functional composition for skin whitening of the present invention In step (S21), it is preferable to ferment by applying only to fennel and white neck mushrooms, excluding centella.

Specifically, the second fermentation broth may be the same as or different from the first fermentation broth, and when the second fermentation broth is different from the first fermentation broth, Bifidobacterium bifidum, Bifidobacterium adolescentis ( Bifidobacterium adolescentis) in a 1:1 (w/w) combination of lactic acid bacteria is preferred. Through the step (S21), the extraction efficiency of the active ingredients in the fennel and white mushrooms can be further increased.

(S3) Adding fennel, white throat mushroom, and centella fermented in step (S2) or step (S21) to the extraction solvent

The method for producing extracts of fennel, white throat mushroom, and centella asiatica according to the present invention includes adding fennel, white neck mushroom, and centella fermented in step (S2) or step (S21) to an extraction solvent having a pH of 6 to 8 (S3). steps may be included. The pH of the extraction solvent may be preferably 6 to 7, more preferably 6.8 to 7, and the extraction efficiency of fermented fennel, white throat mushroom, and centella asiatica may be the highest within the above range.

Specifically, the extraction solvent may be a mixture of methanol, ethanol and ethyl acetate in a ratio of volume of 1:1:2 to 1:2:4 (v/v/v). If the volume ratio of the extraction solvent is out of the above numerical range, the efficiency of extracting the active ingredients contained in the fermented fennel, white throat mushroom, and centella asiatica may be lowered.

(S4) preparing preliminary fennel, white throat mushroom, centella asiatica extracts using an ultrasonic extraction method

In the method for producing extracts of fennel, white throat mushroom, and centella asiatica according to the present invention, (S4) ultrasonic waves are applied to the extraction solvent to which the fermented fennel, white throat mushroom, and centella asiatica are added at 20 to 50 kHz and 0.5 to 40 ° C. It may include a step of irradiating for 1.5 hours to prepare preliminary fennel, white throat mushroom, and Centella asiatica extracts.

It is preferable to irradiate ultrasonic waves at 20 to 35 kHz at 20 to 30 ° C for 0.5 to 1 hour to the extraction solvent to which the fermented fennel, white throat mushroom, and centella asiatica are added, and at 20 to 25 kHz at 25 to 28 ° C and 0.5 to 28 ° C. It is more preferable to irradiate for 0.8 hours. Active ingredients contained in fermented fennel, white throat mushroom, and centella asiatica can be extracted with high efficiency only when the irradiation range of the ultrasonic wave satisfies the frequency, temperature, and time ranges of the numerical range.

Specifically, the step of preparing the preliminary fennel, white neck mushroom, and Centella asiatica extracts using the ultrasonic extraction method has the characteristics of achieving complete extraction and having a very short extraction time. The high-power ultrasound generated through the ultrasonic extraction method alternately generates high-pressure/low-pressure cycles through the extraction solvent to which the fermented fennel, white throat mushroom, and centella asiatica are added, thereby producing It has the advantage of increasing the efficiency of extraction of active ingredients.

(S5) Step of preparing extracts of fennel, white throat mushroom, and centella asiatica

In the method for producing fennel, white neck mushroom, and centella extracts according to the present invention, (S5) filtering the preliminary fennel, white neck mushroom, and centella extracts, and then filtering the preliminary fennel, white neck mushroom, and centella extracts into conventional It may include a step of preparing extracts of fennel, white throat mushroom, and centella asiatica by reducing pressure and concentrating as a method.

Specifically, extraction residues may be removed using filter papers (Whatman, 90mm Dia, 2.5 μm, Whatman international Ltd) to filter the preliminary fennel, white throat mushroom, and Centella asiatica extracts.

Hereinafter, the present invention will be described in detail by the following specific examples. However, the following specific examples are only to illustrate the present invention, and the contents of the present invention are not limited by the following specific examples.

Comparative Preparation Example 1: Obtaining an extract (hot water extraction)

After preparing 100g of fennel and white throat mushrooms with purified water and drying them, put them in 2L of distilled water and heat them at 80 to 100℃ in an extractor equipped with a cooling condenser (product name: Cosmos-660, manufacturer: Kyungseo Machinery) for 2 hours. Each was extracted 3 times in total.

Each extract was filtered with 300 mesh filter paper, and the precipitate was filtered twice with Edvantech No. 5 filter paper and Whatman GFC 150 mm filter paper. After concentrating at a temperature of 40 to 50 ° C using a vacuum condenser (product name: Coolace CCA-1100, manufacturer: EYELA), use a spray dryer (model name: B-290, manufactured by BUCHI) to inlet Temperature: 180℃ Aspirator efficiency: 100% (35cm3/hr), Pump efficiency: 25% (7.5ml/min), Nozzle Cleaner 4 and Rotameter: 30mm (357 liters/hour) to obtain 5 g of an extracted powder.

Preparation Example 2: Obtaining an extract (fermentation)

Fennel, white throat mushroom, and 1 kg each of Centella asiatica were coarsely pulverized with a grinder, finely pulverized using a mass colloider, and heat-treated at 130 ° C. for 10 to 20 minutes. 40 g of drinking water was mixed with 10 g each of fennel, white neck mushroom, and centella obtained through the above process, sterilized twice for 10 minutes at 100 ° C. under 1.0 atm, and cooled to room temperature to prepare fennel, white neck mushroom, and centella to ferment. .

Thereafter, a 1:1 (w/w) mixture of Lactobacillus acidophilus and Lactobacillus plantarum was inoculated into fennel, white throat mushroom, and centella asiatica, respectively, and incubated at 40 ° C for 24 days. Fermented for an hour. At this time, the complex lactic acid bacteria was adjusted to 1% (w / w) relative to the weight of fennel, white throat mushroom, and Centella asiatica.

On the other hand, fennel and white-necked mushrooms were separately refrigerated at 0 ° C for 24 hours, and then mixed with Bifidobacterium adolescentis at a ratio of 1: 1 (w / w). Further fermented for an hour.

Then, the fermented fennel, white throat mushroom, and centella asiatica were added to an extraction solvent (100 mL, pH 7.0, 25 ° C.) in which methanol, ethanol and ethyl acetate were mixed at a ratio of 1: 1: 2 (v / v / v).

Preliminary fennel, white neck mushroom, and centella extracts were prepared by irradiating ultrasonic waves (20 kHz, 750 watt, 20 amplitude) on the fennel, white neck mushroom, and centella asiatica at 25 ° C. for 40 minutes in the extraction solvent. After removing the extraction residue from the preliminary fennel, white throat mushroom, and centella extracts using filter papers (Filter papers whatman, 90mm Dia, 2.5 μm, Whatman international Ltd), the resultant was filtered and concentrated under reduced pressure to finally remove the fennel and white throat. Mushroom and centella extract powders were prepared.

Comparative Examples 1 and 2

Each of the fennel extract powder (Comparative Example 1) and the white throat extract powder (Comparative Example 2) prepared in Comparative Preparation Example 1 was heated at 90 to 100 ° C. for 2 hours by adding 10 to 20 times the volume of water per dry weight. After heating at 50 to 60 ° C. for 12 hours, it was cooled. After cooling, in order to remove floating microparticles, the extract was filtered through a 1 μm microfilter to obtain the extracts of Comparative Examples 1 and 2.

Experimental Examples 1-1 to 1-5 (fennel extract and white throat mushroom extract)

After mixing the fennel extract powder and white throat mushroom extract powder prepared in Preparation Example 2 in the weight ratio shown in Table 1 below, 10 to 20 times the volume of water per dry weight was added and heated at 90 to 100 ° C. for 2 hours. After heating at 50 to 60 ° C. for 12 hours, it was cooled. After cooling, in order to remove floating microparticles, the extract was filtered through a 1 μm microfilter to obtain extracts of Test Examples 1-1 to 1-5.

division Fennel (% by weight) White Thrush Mushrooms (% by weight) Test Example 1-1 5 One Test Example 1-2 3 One Test Example 1-3 One One Test Example 1-4 One 3 Test Example 1-5 One 5

Test Examples 2-1 to 2-4 (centella extract)

After mixing each centella extract powder prepared in Preparation Example 2 in the weight ratio shown in Table 2 below, 10 to 20 times the volume of water per dry weight was added, heated at 90 to 100 ° C. for 2 hours, and then heated at 50 to 60 ° C. After heating for 12 hours, it was cooled. After cooling, in order to remove floating microparticles, the extract was filtered through a 1 μm microfilter to obtain Test Examples 2-1 to 2-4.

division Fennel (% by weight) White Thrush Mushrooms (% by weight) Centella asiatica (% by weight) Test Example 2-1 3 One 5 Test Example 2-2 3 One 2.5 Test Example 2-3 3 One One Test Example 2-4 3 One 2

Experimental Example 1: Cell proliferation and cytotoxicity evaluation test

Experimental Examples 1-1 to 1-5 and 2-1 to 2-4 were diluted to a concentration of 10 μg/ml, and MTT irradiation was performed using samples diluted to evaluate cell proliferation and cytotoxicity. The results are shown in Table 4 below.

MTT (3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide) is a pale yellow substrate that is cleaved by respiratory chain enzymes in the mitochondria of living cells to form dark blue formazan. (formazan). Since the reaction does not occur in dead cells, the amount of formazan produced is used to measure the number of living cells.

The experiment was conducted without adding the extract as a control group, and the cell survival rate was calculated based on the control group.

sample Cell survival rate (%) control group 100 Test Example 1-1 95 Test Example 1-2 97 Test Example 1-3 96 Test Example 1-4 96 Test Example 1-5 95 Test Example 2-1 93 Test Example 2-2 92 Test Example 2-3 94 Test Example 2-4 95

According to the results of Table 3, it can be confirmed that the extract of the test example does not exhibit a cytotoxic effect.

Experimental Example 2: Confirmation of anti-wrinkle and improvement effects

2 ml of DMEM culture medium was put in a 40 mm cell culture dish, and human fibroblasts were inoculated at a concentration of about 1.2x10 ⁵ , and cultured for 24 hours in a 37°C 5% CO ₂ environment. Thereafter, the extracts prepared in Comparative Examples, Test Examples 1-1 to 1-5, and Test Examples 2-1 to 2-4 were replaced with media containing samples diluted at a concentration of 10 μg/ml and cultured for 3 days. Thereafter, the culture solution was harvested and centrifuged for 5 minutes in an environment of 4 ° C and 7500 rpm, and the ELISA method was used to obtain MMP-1 (Human Total MMP-1 kit, R&D Systems, Inc., Minneapolis, MN, USA). Changes in protein expression levels were confirmed. For comparative evaluation, TGF-β was used as a control. The results are shown in Table 4 below.

sample MMP-1 production (ng/ml) control group 3,012 Comparative Example 1 3,217 Comparative Example 2 3,129 Test Example 1-1 2,787 Test Example 1-2 2,713 Test Example 1-3 2,798 Test Example 1-4 2,858 Test Example 1-5 2,802 Test Example 2-1 2,701 Test Example 2-2 2,657 Test Example 2-3 2,661 Test Example 2-4 2,612

According to the results in Table 4, it was confirmed that the effect of reducing MMP-1 protein expression in the sample containing the fennel extract and the white-throated mushroom extract was significantly superior to the case of using them individually, and the sample further included centella asiatica. It was confirmed that the effect of reducing MMP-1 protein expression was measured higher.

This indicates that the fennel extract, the white throat mushroom extract, and the Centella asiatica extract effectively inhibit the production of MMP-1 protein, which is the cause of wrinkles, and thus have excellent effects in preventing and improving skin wrinkles.

Experimental Example 3: Confirmation of whitening effect

After treating melan-A cells with samples obtained by diluting the extracts prepared in Comparative Examples, Test Examples 1-1 to 1-5, and Test Examples 2-1 to 2-4 at a concentration of 10 μg/ml for 3 days, The medium was removed and washed twice with PBS (phosphate buffer saline). Then, in order to dissolve melanin, 400 μl of 1N NaOH was added, and the melanin content was measured at an absorption wavelength of 405 nm using a microplate reader (Hosoi et al., 1985). For comparative evaluation, arbutin known to have a skin whitening effect was used as a control group. The results are shown in Table 5 below.

sample Melanin pigment level (Effective ratio (% of Normal)) control group 78 Comparative Example 1 69 Comparative Example 2 71 Test Example 1-1 53 Test Example 1-2 54 Test Example 1-3 53 Test Example 1-4 57 Test Example 1-5 55 Test Example 2-1 51 Test Example 2-2 59 Test Example 2-3 58 Test Example 2-4 57

According to the results of Table 5, it was confirmed that the samples containing the fennel extract and the white throat mushroom extract had significantly better melanin production inhibitory effect than when they were used individually, and the effect was equal or superior even when compared to arbutin used as a control group. Since it was confirmed that there is, it can be used very effectively for purposes related to skin whitening.

Experimental Example 4: Confirmation of antioxidant effect

180ul of 0.2mM DPPH solution dissolved in methanol and 20ul of distilled water were diluted to a concentration of 10μg/ml of the extracts prepared in Comparative Examples, Test Examples 1-1 to 1-5, and Test Examples 2-1 to 2-4 dissolved in 20ul of distilled water. and vitamin C (control) (100 μg/ml) were placed in a 96-well microplate, incubated at 37°C for 30 minutes, and the amount of reduced DPPH was measured using a spectrophotometer (Precision microplate reader; Molecular Devices, VersaMax). The antioxidant activity was compared by measuring the absorbance at 520 nm. When DPPH reacts with a substance having antioxidant activity, radicals are scavenged and discolored to dark purple, so it is widely used to measure the hydrogen donating ability of antioxidant substances through absorbance measurement. This experiment was repeated three times (Sang S et al., J. Agric. Food Chem. 50(8), p2459-2463, 2002). The results are shown in Table 6 below.

sample Effective ratio (% of normal) (ROS) control group 58 Comparative Example 1 80 Comparative Example 2 81 Test Example 1-1 65 Test Example 1-2 63 Test Example 1-3 64 Test Example 1-4 70 Test Example 1-5 69 Test Example 2-1 63 Test Example 2-2 62 Test Example 2-3 60 Test Example 2-4 62

According to the results of Table 6, the samples containing the fennel extract and the white-throated mushroom extract were found to have antioxidant activity of scavenging free radicals equal to or superior to vitamin C, which is known to have an excellent antioxidant effect used as a control. It can be used very effectively for a function-related purpose.

Experimental Example 5: Confirmation of moisturizing effect

Male, hairless mice aged 8 to 10 weeks were purchased, raised for 10 days, and then used. During the breeding period, the experimental animals were raised in groups of 3, and feed was provided exclusively for mice.

The extracts prepared in Comparative Examples, Test Examples 1-1 to 1-5, and Test Examples 2-1 to 2-4 were orally administered to mice, and the administration period was 5 weeks, 3 days a week.

UV irradiation was performed three times a week for 5 weeks in Test Examples 1-1 to 1-5 and Test Examples 2-1 to 2-4, and a UV lamp was used for ultraviolet rays. The amount of UV irradiation was 50 mJ/cm ² for 1 to 3 weeks and 75 mJ/cm ² for 4 to 5 weeks for 5 weeks.

The amount of moisture in the skin was measured using a corneometer (Corneometer Courage & Khazaka, Germany) equipment. As shown in Table 7, Test Examples 1-1 to 1-5 and Test Examples 2-1 to 2-4 showed higher water content (Capacitance (AU)) than the ultraviolet (UV) irradiation group. On the other hand, it can be seen that the comparative example has a similar or not significant difference in moisture content compared to the ultraviolet irradiation group.

sample Capacitance (AU) control group 58 UV irradiation group 20 Comparative Example 1 26 Comparative Example 2 24 Test Example 1-1 60 Test Example 1-2 62 Test Example 1-3 63 Test Example 1-4 59 Test Example 1-5 58 Test Example 2-1 63 Test Example 2-2 62 Test Example 2-3 60 Test Example 2-4 64

Although the present invention has been described in detail only with respect to the specific embodiments described above, it is obvious to those skilled in the art that various changes and modifications are possible within the scope of the technical spirit of the present invention, and it is natural that such changes and modifications fall within the scope of the appended claims.

Claims (5)

Preparing a first fermentation broth;
fermenting fennel and white throat mushrooms by adding 0.1 to 0.2% by weight of salt relative to the weight of fennel and white throat mushrooms in the first fermentation broth;
Adding the fermented fennel and white throat mushroom to an extraction solvent having a pH of 6 to 8;
Preparing preliminary fennel and white throat mushroom extracts by irradiating ultrasonic waves at 20 to 50 kHz and 25 to 40 ° C. for 0.5 to 1.5 hours to the extraction solvent to which the fermented fennel and white throat mushrooms are added; and
preparing fennel and white throat mushroom extracts by filtering the preliminary fennel and white throat mushroom extracts and then concentrating the filtered preliminary fennel and white throat mushroom extracts under reduced pressure; including,
Manufacturing method of fennel extract and white throat mushroom extract.
A functional composition for skin whitening comprising the fennel extract and the white throat mushroom extract prepared according to claim 1 as active ingredients.
The functional composition for skin whitening according to claim 2, wherein the fennel extract and the white throat mushroom extract are included in a weight ratio of 5:1 to 1:5, respectively.
The functional composition for skin whitening according to claim 3, further comprising a centella asiatica extract.
The functional composition for skin whitening according to claim 4, wherein the centella asiatica extract is included in an amount of 20 to 70% by weight based on the total weight of the fennel extract and the white throat mushroom extract.