KR101805388B1 - Skin care cosmetic composition and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a cosmetic and skincare composition and a preparing method thereof. More specifically, the cosmetic and skincare composition inoculated and ferments Tremella fuciformis, Acacia senegal, and Diospyros kaki in microorganisms of Rhizobium species. According to the present invention, the composition, which is derived from natural substances and is without cytotoxicity, has excellent antioxidant, anti-aging, and skin regeneration functions to be used as a functional cosmetic composition of antioxidative and anti-aging functions, and more particularly, of skin regenerating and anti-wrinkle functions, thereby being useful in the cosmetics industry.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a skin cosmetic composition,

The invention skin cosmetic composition and as it relates to a process for the preparation, and more specifically, the separation tank emptying in (Rhizobium spp.) microorganisms inoculated and fermented into white throat mushroom, acacia senegal gum and persimmon leaves, and a method for producing the same.

Skin is a part of the body that is directly exposed to the external environment. It is exposed to ultraviolet rays caused by exposure to sunlight in daily life, air pollutants such as yellow dust or automobile soot, and various bacteria, thereby causing skin trouble and irritation. . Such aging of the skin reduces the elasticity of the skin on the appearance of the skin to form wrinkles or to form spots and senile spots to cause skin pigmentation.

In general, aging of the skin is accompanied by various theories such as somatic mutation, excessive production of free radicals, autoimmune reaction, accumulation of toxic substances, errors generated during gene replication, transformation of dendritic proteins, And the diseases caused by various diseases such as adult diseases are attributed to reactive oxygen species (ROS) is recognized that the active oxygen species originating from oxygen and nitrogen to control the natural antioxidants to develop active research is progressing have. The reactive oxygen species, usually known as harmful oxygen, is a lipid peroxide such as peroxide anion radical, hydrogen peroxide, hydroxy radical, peroxidized lipid or free radical generated in the most stable form, It occurs even under normal conditions, but the presence of antioxidant enzymes such as superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and the like inhibit the production of excessive reactive oxygen species. However, when the active oxygen species is abnormally excessive due to stress and external environmental factors and is not eliminated, the oxidative stress due to the free radicals is added to the living body and DNA, RNA, protein, Cell membrane and cell structure damage, cancer, tissue damage, and aging.

Studies on antioxidants capable of controlling or eliminating these active oxygen species have been actively conducted in order to suppress the rapid increase of various diseases and aging in the modern age. Studies on the efficacy of natural antioxidants such as tocopherol, ascorbic acid, carotenoids, flavonoids and glutathione, and their stabilization techniques, and studies on the synthetic antioxidants butylated hydroxytoluene (BHT), butylated hydroxyanisol (BHA), and many other antioxidants have been continuously studied. Among these antioxidants, tocopherol has a relatively low antioxidative effect, and synthetic antioxidants such as BHA and BHT, which are highly effective, are used in pharmaceuticals and foods, but there is a point of mutagenicity and toxicity, and in the case of ascorbic acid, Development of the obtained derivatives and formulations is progressing, and development of a natural antioxidant material having more stable and effective effect is urgently required.

In order to solve such a problem, many studies have been made on a cosmetic composition using an extract which is relatively low in toxicity, safe and effective for skin, among phytochemicals, It has been required to develop a material which maximizes its efficacy since the effect of the ingredient is limited.

On the other hand, in the case of natural extracts through fermentation, sugar components present in natural products are converted into alcohols as carbon sources in the metabolic process of microorganisms without using harmful organic solvents, so that the active ingredients in natural products, especially flavonoids, Polyphenols, catechins, etc. are present as glycated and stable compounds, and the extractability of the active ingredient is increased through fermentation, and excellent skin efficacy is expected by the active ingredient which is excluded from sugar and activated.

KR 10-2013-0038104 A KR 10-1286133 B KR 10-2016-0036118 A KR 10-2000-0039833 A

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in order to solve the problems of the prior art as described above, and it is an object of the present invention to provide a microorganism of the genus Rhizobium which is derived from a natural product having no cytotoxicity and has excellent wrinkle, whitening, skin elasticity, And a method for producing the functional cosmetic composition.

SUMMARY OF THE INVENTION The present invention has been conceived in order to solve the above-mentioned problems, and the present invention provides a method of manufacturing a skin cosmetic composition comprising the steps of:

(1) Rhizobium spp.) inoculating and culturing microorganisms on sterilized white throat mushroom, acacia senegal gum and persimmon leaves;

(2) liposomizing the result of step (1);

(3) mixing aloe extract and ceramide into the result of step (2); And

(4) preparing the result of step (3) as a cosmetic formulation.

The Rhizobium spp.) microorganism is preferably Rhizobium radiobacter .

Preferably, the culturing conditions are a temperature of 20 to 40 DEG C, a relative humidity of 70 to 90%, a pH of 5.4 to 6.8, and a culture period of 1 to 5 days.

In addition, the present invention provides a skin cosmetic composition prepared by the method of the present invention.

The cosmetic composition is preferably a formulation selected from the group consisting of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a powder foundation, an emulsion foundation, a wax foundation, a pack, a spray, a powder and a lot.

The cosmetic composition for skin care of the present invention is a composition derived from a natural product having no cytotoxicity, and is excellent in antioxidation, inhibition of skin aging and promoting skin regeneration, and thus is applied as a functional cosmetic composition for antioxidant and anti- It is very useful in the cosmetics industry.

FIG. 1 is a flow chart showing a manufacturing process of the skin cosmetic composition of the present invention.

Hereinafter, the present invention will be described in detail.

The inventors of the present invention have conducted research to develop a material for a functional cosmetic composition using a natural product. As a result, they have found that a microorganism belonging to the genus Rhizobium obtained by inoculating a microorganism belonging to the genus Rhizobium into a white mushroom, an acacia senegal gum and a persimmon leaf, It is confirmed that the natural fermented product has excellent skin elasticity enhancement effect, wrinkle improvement, whitening effect and the like, and that the skin cosmetic composition of the present invention can be used as a functional cosmetic composition.

Accordingly, the present invention provides a method of making a skin cosmetic composition comprising the steps of:

(1) Rhizobium spp.) inoculating and culturing microorganisms on sterilized white throat mushroom, acacia senegal gum and persimmon leaves;

(2) liposomizing the result of step (1);

(3) mixing aloe extract and ceramide into the result of step (2); And

(4) preparing the result of step (3) as a cosmetic formulation.

As a microorganism used for fermenting natural products of the present invention, the microorganism of Rizobium is a bacterium which makes roots at roots of legumes and plants, and fixes nitrogen in air while symbiosis with plants. When nitrogen compounds are supplied to legumes, Supplies carbon and other bacterial growth materials. There are 1-year-old rootstock and perennial rootstock, each of which has a unique appearance and structure depending on the type of plant to be parasitized. The kind of root-hole bacteria depends on the kind of bean and plant in which the bacteria parasitize. In 1888, when MW Bayerlink of the Netherlands separated this bacterium purely, this bacterium was thought to be only one species, but now it is said to be parasitic in pea, parasitic in magpie, parasitic in antiquity, Seven species are known, including parasitism in the genus and parasitism in the bean. In early bacterium, the root bacterium exists in the form of glandular pseudotypic bacterium in addition to the liver germ. In later stages, vacuoles are formed, and small bacterium and egg form are formed, and the morphology changes greatly. It grows at 0 ~ 50 ℃ and develops best at 20 ~ 28 ℃. Soil of pH 5.4 ~ 6.8 is best suited for long-term survival in soil. Because it is aerobic, there are many in the surface layer of the soil, and the root is also abundant in the topsoil part of the parasitic plant.

In the present invention, the Rhizobium spp.) as the microorganism can be used for separation tank emptying radio bakteo (Rhizobium radiobacter), or separation tank during emptying trophy (R. tropici), preferably, the separation tank emptying radio bakteo (Rhizobium radiobacter .

The Rhizobium microorganism of the present invention can be produced by seed culture of a Rhizobium species microorganism. The seeds of the respiratory microorganisms may be periodically subcultured until they are used. As a preferable example, microorganisms of the genus Rhizobium are inoculated on a PDA (Potato Dextrose Agar) medium and incubated for about 4 weeks Followed by subculturing at intervals.

The microorganism of the respirometes cultured subculture can be obtained as a liquid culture by inoculating a liquid culture medium with a part of the culture medium of the respirometric microorganisms to be subcultured and then cultivating the culture in a rotary shake.

The substrate used for the fermentation of natural products of the present invention is pure white and translucent gelatinous substance, like a chicken crest or a group of petiole-like petals, having a diameter of 3 to 8 cm and a height of 2 to 5 cm, It has the characteristic of holding and hardening. The fascial layer develops on the surface, and the quartz is a spherical form of egg, with a diameter of 10 ~ 13 ㎛ and divided into 4 chambers by vertical partition. The size of the spores is 6 ~ 8 × 5 ~ 6㎛, almost ovate.

In the present invention, a fermented product is obtained by inoculating a microorganism belonging to the genus Rhizobium with white mushroom, and the fermented product is excellent in skin elasticity and wrinkle-reducing effect.

Acacia senegal black arabic gum, which is a substrate used for the fermentation of natural products of the present invention, and is not pulverized, is a round-shaped lump or angular piece having various sizes of white-yellowish white, or a white-yellowish white flake, It is in powder form. Obtained by drying Arabian black beans and extracts of Arabian rubber tree (Acacia senegal WILLDENOW) or other plants, or by desalting it, and the main component is polysaccharide. When water is added, it gradually dissolves to show acidity. It becomes a thick viscous liquid with no taste to 50% aqueous solution, and its viscosity decreases over time. In particular, stable emulsions can be obtained over a wide pH range, and o / w emulsification is possible even with an electrolyte such as a salt.

In the present invention, acacia senegal gum is inoculated with a microorganism of the genus Rhizobium to obtain a fermented product. Such a fermented product helps improve skin elasticity.

The persimmon leaf used as a substrate for fermenting natural products according to the present invention can be used by drying young leaves or adult leaves to powder, and an excellent effect on whitening effect can be obtained.

In step (1), 3 to 50 parts by weight of acacia senegal gum and 10 to 30 parts by weight of persimmon leaves may be mixed with 100 parts by weight of white mushroom, acacia senegal gum and persimmon leaf, which are fermentation substrates, of white mushroom.

The fermentation substrate may further be supplied with a carbon source and a nitrogen source. The amount of the inoculum of the microorganism of the present invention may be 1 to 5 parts by weight of the present culture medium of the microorganism of the genus Rhizobium in relation to 100 parts by weight of the fermentation substrate but may be suitably adjusted according to the concentration of the culture medium, It is obvious to those who have.

Preferably, the culturing conditions are a temperature of 20 to 40 DEG C, a relative humidity of 70 to 90%, a pH of 5.4 to 6.8, and a culture period of 1 to 5 days.

The natural fermentation product using the produced microorganism of the present invention is liposomized to increase the skin absorption rate.

Liposomes refer to lipid vesicles (artificial membranes). When a phospholipid is suspended in a salt aqueous solution, a multilayer liposome is formed. When the liposome is ultrasonicated, a vesicle having a two-minute caption is obtained. The method of enclosing the natural fermentation product using the microorganism of the present invention in liposomes is well known in the technical field of the present invention.

The cosmetic composition of the present invention is intended to improve the skin moisturizing effect by further mixing the aloe extract and the ceramide in addition to the natural fermentation product using the microorganism of the present invention.

The aloe extract and ceramide may be mixed in an amount of 10 to 20 parts by weight based on 100 parts by weight of the natural fermentation product using the liposomized Rhesus microorganism.

The main components of the cosmetic composition of the present invention can be prepared in various formulations.

In addition to the main ingredients described above in the preparation of a desired formulation, known natural extracts and other ingredients for the effects of antioxidation, skin aging prevention, and promotion of skin regeneration may be further added and used within the range not inhibiting the active activity of the present invention have.

The formulations may also contain components commonly added to cosmetic compositions such as fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, A cosmetic or dermatological composition such as a perfume, a perfume, a surfactant, water, an ionic or nonionic type emulsifier, a filler, a sequestering and chelating agent, a preservative, a vitamin, a barrier, a wetting agent, an essential oil, a dye, a pigment, a hydrophilic or lipophilic active agent May be formulated into a particular formulation, including adjuvants or carriers commonly used in the field of dermatology.

The skin cosmetic composition of the present invention can be provided by the above-described manufacturing method. Accordingly, the present invention provides a skin cosmetic composition prepared by the method of the present invention.

The cosmetic composition is preferably a formulation selected from the group consisting of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a powder foundation, an emulsion foundation, a wax foundation, a pack, a spray, a powder and a lot.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as the carrier component. In particular, in the case of a spray, a mixture of chlorofluorohydrocarbons, Propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

Hereinafter, the present invention will be described in more detail with reference to preferred embodiments. The following examples illustrate one preferred embodiment of the present invention and are not to be construed as limiting the scope of the present invention.

[ Example ]

Example  One. Risibium  Natural microorganism Fermented  Produce

Rhizobium Radiobacter ) was cultured using a PDA (Potato Dextrose Agar, Difco, Co., USA) medium at 25 ° C using subculture strains, and stored at 4 ° C while subculturing at intervals of 4 weeks. To a 500 ml flask was added 100 ml of a liquid culture medium containing Rhizobium radiobacter seed cultures were inoculated and cultured for 3 days in a rotary shaking incubator (Daihan Labtech Co., Ltd., 25 ± 1 ° C., 130 rpm) to be used as liquid seeds. White stover mushroom was purchased from Kyungdong market medicine, washed with water before removing impurities and dehydrated. 100 g of white mushroom was placed in a 3 L culture container, sterilized at 121 캜 for 30 minutes, and then cooled at room temperature. 30 g of acacia senegal gum was placed in a 1 L culture container, sterilized at 121 캜 for 30 minutes under high pressure, and then cooled at room temperature. Persimmon leaves were purchased from Kyungdong market medicines, washed with water to remove impurities, and dehydrated. 20 g of the persimmon leaves were placed in a 1 L culture container, sterilized at 121 ° C for 30 minutes, and then cooled at room temperature. 5 ml of the liquid seed thus prepared was aseptically inoculated into 100 g of sterilized white mushroom, 30 g of acacia senegal, and 20 g of persimmon leaf powder. Fermentation was carried out for 3 days in an incubator at 25 ° C and 80% relative humidity. After fermentation, 10 times volume of hot water was added, and the mixture was shaken for 2 hours and then immersed for 72 hours. After centrifugation at 10,000 rpm for 10 minutes, the supernatant was taken. After repeating this three times, the extract was concentrated under reduced pressure to prepare a powder Respectively.

Example  2. Beauty Cosmetics  Manufacturing of formulations

Using the sample prepared in Example 1, a cream formulation was prepared with the following ingredients.

ingredient Composition ratio (%) Purified water 48.5 Incrementer 0.5 Moisturizer 10 Emulsifying stabilizer 10 vegetable oil 10 Beads wax 5 Silicone oil 5 incense One sample 10

Experimental Example  1. Validation of antioxidant activity

The antioxidant activity of the sample prepared in Example 1 was evaluated. The prepared sample (0.5 mg / ml) was evaluated by DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging ability and FRAP activity measurement. As a control, a hot-water extract of white throat mushroom / acacia senegal gum / persimmon leaves, which was not fermented by Ribium radiobacter, was used.

For DPPH scavenging ability, 380 μl of 2 × 10 ^-4 M DPPH solution dissolved in 99.5% ethanol was added to 20 μl of diluted samples at various concentrations, and the mixture was reacted at 37 ° C. for 30 minutes. Then, microplate reader (Asys Hitech, Expert 96, Asys Co., Austria). DPPH free radical scavenging activity was expressed as a percentage as shown in Table 2 below.

activation sample Control DPPH radical scavenging activity (%) 12.03 + - 0.40 7.42 + - 1.23

FRAP activity was performed according to a known method on the prepared sample (0.5 mg / ml).

activation sample Control FRAP (% of control) 26.51 + - 0.57 15.32 ± 0.18

Experimental Example  2. Skin application test

The cream formulations prepared in Example 2 were applied to the skin of 30 adult men and women at intervals of 3 times / month for 1 month, and the skin change patterns according to the following evaluation items were evaluated. As a control, cream formulations prepared from hot water extract samples of white throat mushroom / acacia senegal gum / persimmon leaves which were not fermented with Ribium radiobacter was used. The evaluation was performed according to the 5-point scaling method and the results are shown in Table 4 below.

Evaluation items sample Control elasticity 4.5 ± 0.10 3.2 ± 0.59 wrinkle improvement 4.6 ± 0.54 3.0 ± 0.64 Whitening 4.7 ± 0.02 2.8 ± 0.31 Overall rating 4.6 ± 0.32 3.1 ± 0.20

Claims (5)

(1) inoculating and fermenting Rhizobium sp. Microorganisms into sterilized white throat mushroom, acacia senegal gum and persimmon leaves;
(2) preparing the result of step (1) as a cosmetic formulation,
The Rhizobium spp. Microorganism is Rhizobium radiobacter ,
Wherein the fermentation condition is a temperature of 20 to 40 DEG C, a relative humidity of 70 to 90%, a pH of 5.4 to 6.8, and a fermentation period of 1 to 5 days.
delete delete A cosmetic composition for skin care produced by the process according to claim 1.
The cosmetic composition according to claim 4, wherein the cosmetic composition is selected from the group consisting of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a powder foundation, an emulsion foundation, a wax foundation, a pack, a spray, Wherein the composition is a formulation.

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