CN107997985A - Application of the deerskin polysaccharide in moisture-keeping cosmetics are prepared - Google Patents
Application of the deerskin polysaccharide in moisture-keeping cosmetics are prepared Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/007—Preparations for dry skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- C—CHEMISTRY; METALLURGY
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
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Abstract
The present invention relates to application of the deerskin polysaccharide in moisture-keeping cosmetics are prepared, and belongs to a kind of application of medicinal economic animal bioactive polysaccharide in cosmetics preparation field.Deerskin polysaccharide has nutrition, anti-aging, stable emulsion, antibacterial anti-inflammatory, promotion wound healing and repairs the specific functions such as damaged cell, transdermal delivery can also be played the role of to other activity or nutritional ingredient in skin care item at the same time, it is greatly promoted absorption of the skin to these active ingredients, make skin moisturizing ability stronger, peel so as to significantly improve skin caused by drying, the symptom such as neurodermatitis, contact dermatitis and solar dermatitis.Commonly using the cosmetics containing deerskin polysaccharide, skin can be made finer and smoother, moist, soft and smooth and full of elasticity.
Description
Technical field
The present invention relates to a kind of application of medicinal economic animal polysaccharide, especially sika deer deerskin polysaccharide to prepare moisturizing
Application in cosmetic field.
Technical background
Deer is one of important medicinal economic animal in China, with its exclusive medical value and medical health care function increasingly by
People are of interest.The provinces and regions such as three provinces in the northeast of China of China, Xinjiang, Inner Mongol, Shandong, Shanxi sika deer aboundresources, but with pilose antler, deer
The product markets such as tire, deer whip and venison are flourished, and the deep processing of the accessory substance such as deerskin is also extremely urgent.At present, deerskin produces
Product are mainly that related deerskin is eaten, medicinal and health product developmental research is relatively fewer leather goods article is made after tanning,
And the extraction of deerskin polysaccharide and the research in cosmetic field are even more in blank with application.
The content of the invention
The present invention provides a kind of application of deerskin polysaccharide in moisturizingization hair cosmetic is prepared.Its advantage is to use vacuum and low temperature
Frozen dried deerskin, can retain the active skull cap components of deerskin polysaccharide to the utmost, so that the makeup containing deerskin polysaccharide
Product, which not only have, to be kept and strengthens cuticula itself water absorbing capacity and barrier function, can also change automatically adjusting according to ambient humidity
Optimal moisture-holding capacity, makes the water content of keratoderma be maintained at optimal physiological status, while to neurodermatitis, contact dermatitis
And solar dermatitis etc. has significant curative effect.
Application of the deerskin polysaccharide in moisturizingization hair cosmetic is prepared.
Application of the deerskin polysaccharide in the cosmetics for preparing treatment neurodermatitis, contact dermatitis and solar dermatitis.
Deerskin polysaccharide of the present invention, is extracted by following technical proposal.
(1), deerskin is pre-processed, including loses hair or feathers, cleans, sterilizes, is freeze-dried, crushes, add petroleum ether degreasing.Specially
The clean deerskin that will first lose hair or feathers cuts into granularity and is less than 0.5cm2Fragment, sterilize 1-2min, be first placed in -20 DEG C of low temperature refrigerator
In quick-frozen 9-10h, then be placed in vacuum freeze drier progress low temperature frozen dried, after freezing using high-speed tissue mashing machine with
The rotating speed of 12000r/min carries out break process 3-5min, obtains deerskin powder, then adds petroleum ether degreasing, surname extraction 24h, puts
Room temperature treats that petroleum ether volatilization completely is saved backup after in 4 DEG C of refrigerators.
(2), to the deerskin powder after degreasing, according to 1:70 solid-liquid ratios add distilled water, in ultrasonic power 450-500W, carry
Ultrasonic extraction 50-60min under the conditions of 85-90 DEG C of temperature is taken, then with 1mol/LNaOH tune pH value to 7.0, adds deerskin powder
Papain, 2.5-3.0% trypsase, 1.0-1.5% alkali proteases and the 1.0-1.5% neutrality eggs of quality 2.5-3.0%
White enzyme, digests 2-3h in 45-50 DEG C of water bath, and enzymolysis liquid finally is placed in 100 DEG C of water-bath high temperature enzyme deactivation 15min.
(3), to the extracting solution removing protein after enzyme deactivation.Concrete operations:5-8min is centrifuged with 5000r/min, takes supernatant to subtract
Pressure is concentrated into the half of original volume, by concentrate and solution of trichloroacetic acid(By volume 15:1)It is uniformly mixed, sinks in 4 DEG C of environment
Centrifugal filtration after the 3h of shallow lake, collects filtrate.
(4), to the filtrate after removing protein, be initially charged with the bag filter that molecular cut off is 3500Da respectively with tap water and
Successively each 24h that dialyses of distilled water, is concentrated under reduced pressure into the half of original volume, adds 95% ethanol precipitation 12h of 4 times of volumes, with 5000
R/min centrifuges 15min, collects precipitation, is washed repeatedly with absolute ethyl alcohol 3 times, room temperature is dried, the quick-frozen 9-10h of -20 DEG C of low temperature refrigerators
Afterwards, it is dry under the conditions of vacuum 0.08-0.09MPa, -85 DEG C, obtain deerskin Thick many candies.
(5), add appropriate distilled water to redissolve to deerskin Thick many candies, 3% cetyl trimethylammonium bromide solution is added dropwise until heavy
Form sediment complete, stand centrifugal filtration after 12h, precipitation 2mol/L NaCl redissolve, and add 95% ethanol of 3 times of volumes, are received after alcohol precipitation 12h
Collection precipitation, is redissolved with appropriate distilled water, is fitted into the bag filter that molecular cut off is 3500Da, successively with tap water and distilled water
Each dialysis 24h, is concentrated under reduced pressure into after the half of original volume and adds 4 times of 95% ethanol precipitation 12h of volume, collection be deposited in -20 DEG C it is low
Quick-frozen 9-10h in warm refrigerator-freezer, will be deposited in vacuum 0.08-0.09MPa, is freeze-dried under the conditions of -85 DEG C, obtain deerskin and refine
Polysaccharide.
It is white solid powder under refined deerskin polysaccharide room temperature, odorless, tasteless, there is hygroscopicity, is slightly soluble in cold water, is soluble in
Hot water, insoluble in the organic solvents such as ethanol, ether, glucuronic acid content 39.37%, aminoglucose sugared content 41.65%, molecule
Measure 86400Da.
Deerskin polysaccharide of the present invention is used to prepare moisture-keeping cosmetics care human skin, and to neurodermatitis, contact skin
Scorching and solar dermatitis can be prepared into gelling agent, emulsion, paste, spray and facial mask etc. when being treated.
, can also be with addition to including conventional cosmetics carrier when deerskin polysaccharide of the present invention is used to prepare moisture-keeping cosmetics
Other additives coexist, such as surfactant, thickener, moisturizer, stabilizer, aromatic and preservative etc..
Deerskin polysaccharide belongs to animal polysaccharide, and research shows animal polysaccharide for acid glycosaminoglycan, its bioactivity being rich in
Material, not only with anti-inflammatory, antiviral, anti-good fortune the physiological activity such as penetrate, be anti-oxidant, but also skin tissue regeneration can also be promoted, prevent
The only structure change of skin protein, plays the role of moisture-keeping crease-shedding, and to neurodermatitis, contact dermatitis and solar dermatitis
Deng having significant curative effect.
Animal polysaccharide histocyte has lipid to surround, it is necessary to first carry out ungrease treatment more, is carried to improve degreasing rate with polysaccharide
Rate is taken usually to be crushed after raw material drying.Conventional method is typically to be crushed after 60 DEG C or so drying to Animal Skin drying, by
Cause to crush incomplete in Animal Skin water content is higher, not only reduce degreasing rate and polysaccharide extract rate, and dehydration temperaturre is high
Also reduce the bioactivity of polysaccharide material.This research is using the deerskin after super low temperature quick frozen depilation, under the conditions of vacuum and low temperature
Water is directly distilled and frozen dried is carried out to deerskin, so that deerskin is not only dehydrated soon, water content is low, and polysaccharide extract rate is also high,
And vacuum and low temperature is freezed and played a protective role to the bioactive substance in deerskin polysaccharide, while the deerskin that low temperature freezes crushes
Particle fine uniform afterwards, loose porous, density is small, not only drastically increases deerskin polysaccharide extract rate, also protects well
The bioactivity of deerskin polysaccharide.
As requirement of the consumer to cosmetics is increasingly intended to safe, the raw material of cosmetics are also increasingly intended to
Green natural.Deerskin polysaccharide integrates organic natural, security and feature, as the natural activity raw material of cosmetics
Applied to the preparation of cosmetics, its product not only can moisturizing it is anti-ageing, increase elasticity of skin, improve inflammation fragility skin quality, and
The economic benefit of its product is improved, the development and utilization of more deer byproduct resource provides solid theoretical foundation.
The advantage of the invention is that:Providing one kind, more preferably deerskin polysaccharide carries relative to conventional animal polysaccharide extracting process
Technology is taken, and gained is used to prepare deerskin polysaccharide moisture-keeping cosmetics rich in natural bioactive deerskin polysaccharide.Deerskin polysaccharide
With free radical antioxidation is removed, there are moisturizing, anti-inflammatory, antibacterial, anti-infective, antiulcer and improve the functions such as immunity,
There is significant curative effect to neurodermatitis, contact dermatitis and solar dermatitis etc..Containing a large amount of on the monosaccharide residue of its polysaccharide
Hydroxyl, by hydrogen bond, can combine and preserve many hydrones, form a kind of elastomeric network group that can support very big volume
Knit, these hydrones are dissipated due to not volatile, play the remarkable moisturizings such as aquation, lubrication, the sertoli cell movement of conveying solute
Function, so that skin care, and neurodermatitis, contact dermatitis and the solar lentigines skins caused by factor such as dry skin can be suppressed
The inflammation such as inflammation;Deerskin polysaccharide can also remove free radical, anti-infective anti-inflammatory, activation when maintaining dermal connective tissue moisture at the same time
Cell and repair tissue, prevent collagen to be changed into insoluble state from dissolved state.Use the deer containing active skull cap components
After skin polysaccharide moisture-keeping cosmetics, not only moisture retention and trophism are notable, but also anti-inflammatory, antibacterial, crease-resistant effect are obvious, fundamentally
The humidity of skin is maintained, corrects the symptoms such as dry skin, furfur, inflammation, wrinkle, so that skin more water moistens, is elastic, is thin
It is greasy, net white, improve fragile inflammation skin quality conscientiously.
Embodiment.
Embodiment 1.
Deerskin polysaccharide of the present invention, is extracted by following technical proposal.
(1), deerskin is pre-processed, including loses hair or feathers, cleans, sterilizes, is freeze-dried, crushes, add petroleum ether degreasing.Specially
The clean deerskin that will first lose hair or feathers cuts into granularity and is less than 0.5cm2Fragment, after sterilizing 1min, be first placed in -20 DEG C of low temperature refrigerator
In quick-frozen 9h, then be placed in vacuum freeze drier progress low temperature frozen dried, after freezing using high-speed tissue mashing machine with
The rotating speed break process 3min of 12000r/min, obtains deerskin powder, then adds petroleum ether degreasing, surname extraction 24h, puts room temperature and treat
Petroleum ether volatilization completely is saved backup after in 4 DEG C of refrigerators.
(2), to the deerskin powder after degreasing, according to 1:70 solid-liquid ratios add distilled water, in ultrasonic power 450W, extraction temperature
Ultrasonic extraction 50min under the conditions of 85 DEG C of degree, then with 1mol/LNaOH tune pH value to 7.0, adds deerskin powder quality 2.5%
Papain, 2.5% trypsase, 1.5% alkali protease and 1.5% neutral proteinase, digest in 45 DEG C of water baths
2h, is finally placed in 100 DEG C of water-bath high temperature enzyme deactivation 15min by enzymolysis liquid.
(3), to the extracting solution removing protein after enzyme deactivation.Concrete operations:5min is centrifuged with 5000r/min, takes supernatant to depressurize
The half of original volume is concentrated into, by concentrate and solution of trichloroacetic acid(By volume 15:1)It is uniformly mixed, is precipitated in 4 DEG C of environment
Centrifugal filtration after 3h, collects filtrate.
(4), to the filtrate after removing protein, be initially charged with the bag filter that molecular cut off is 3500Da respectively with tap water and
Successively each 24h that dialyses of distilled water, is concentrated under reduced pressure into the half of original volume, adds 95% ethanol precipitation 12h of 4 times of volumes, with 5000
R/min centrifuges 15min, collects precipitation, is washed repeatedly with absolute ethyl alcohol 3 times, and room temperature is dried, after the quick-frozen 9h of -20 DEG C of low temperature refrigerators,
It is dry under the conditions of vacuum 0.08MPa, -85 DEG C, obtain deerskin Thick many candies.
(5), add appropriate distilled water to redissolve to deerskin Thick many candies, 3% cetyl trimethylammonium bromide solution is added dropwise until heavy
Form sediment complete, stand centrifugal filtration after 12h, precipitation 2mol/L NaCl redissolve, and add 95% ethanol of 3 times of volumes, are received after alcohol precipitation 12h
Collection precipitation, is redissolved with appropriate distilled water, is fitted into the bag filter that molecular cut off is 3500Da, successively with tap water and distilled water
Each dialysis 24h, is concentrated under reduced pressure into after the half of original volume and adds 4 times of 95% ethanol precipitation 12h of volume, collection be deposited in -20 DEG C it is low
Quick-frozen 9h in warm refrigerator-freezer, will be deposited in vacuum 0.08MPa, is freeze-dried under the conditions of -85 DEG C, obtain deerskin refined polysaccharide.
Embodiment 2.
(1), deerskin is pre-processed, including loses hair or feathers, cleans, sterilizes, is freeze-dried, crushes, add petroleum ether degreasing.Specially
The clean deerskin that will first lose hair or feathers cuts into granularity and is less than 0.5cm2Fragment, after sterilizing 2min, be first placed in -20 DEG C of Low-temperature Ice
Quick-frozen 10h in cabinet, then be placed in vacuum freeze drier progress low temperature frozen dried, after freezing using high-speed tissue mashing machine with
The rotating speed of 12000r/min carries out break process 5min, obtains deerskin powder, then adds petroleum ether degreasing, surname extraction 24h, puts room
Temperature treats that petroleum ether volatilization completely is saved backup after in 4 DEG C of refrigerators.
(2), to the deerskin powder after degreasing, according to 1:70 solid-liquid ratios add distilled water, in ultrasonic power 500W, extraction temperature
Ultrasonic extraction 60min under the conditions of 90 DEG C of degree, then with 1mol/LNaOH tune pH value to 7.0, adds deerskin powder quality 3.0%
Papain, 3.0% trypsase, 1.0% alkali protease and 1.0% neutral proteinase, digest in 50 DEG C of water baths
3h, is finally placed in 100 DEG C of water-bath high temperature enzyme deactivation 15min by enzymolysis liquid.
(3), to the extracting solution removing protein after enzyme deactivation.Concrete operations:8min is centrifuged with 5000r/min, takes supernatant to depressurize
The half of original volume is concentrated into, by concentrate and solution of trichloroacetic acid(By volume 15:1)It is uniformly mixed, is precipitated in 4 DEG C of environment
Centrifugal filtration after 3h, collects filtrate.
(4), to the filtrate after removing protein, be initially charged with the bag filter that molecular cut off is 3500Da respectively with tap water and
Successively each 24h that dialyses of distilled water, is concentrated under reduced pressure into the half of original volume, adds 95% ethanol precipitation 12h of 4 times of volumes, with 5000r/
Min centrifuge 15min, collect precipitation, washed repeatedly with absolute ethyl alcohol 3 times, room temperature is dried, after the quick-frozen 10h of -20 DEG C of low temperature refrigerators
It is vacuum 0.09MPa, dry under the conditions of -85 DEG C, obtain deerskin Thick many candies.
(5), add appropriate distilled water to redissolve to deerskin Thick many candies, 3% cetyl trimethylammonium bromide solution is added dropwise until heavy
Form sediment complete, stand centrifugal filtration after 12h, precipitation 2mol/L NaCl redissolve, and add 95% ethanol of 3 times of volumes, are received after alcohol precipitation 12h
Collection precipitation, is redissolved with appropriate distilled water, is fitted into the bag filter that molecular cut off is 3500Da, successively with tap water and distilled water
Each dialysis 24h, is concentrated under reduced pressure into after the half of original volume and adds 4 times of 95% ethanol precipitation 12h of volume, collection be deposited in -20 DEG C it is low
Quick-frozen 10h in warm refrigerator-freezer, will be deposited in vacuum 0.09MPa, is freeze-dried under the conditions of -85 DEG C, obtain deerskin refined polysaccharide.
Application Example 1, deerskin polysaccharide moisturizing emulsion.
Formula:It is shown in Table 1.
1 deerskin polysaccharide moisturizing emulsion formula of table.
。
Preparation method:Oil phase component A is first heated with stirring to 80-85 DEG C, insulation 20min sterilizes, then water phase components B is stirred
Mix and be warming up to 85-90 DEG C, insulated sterilizing 15min, homogeneous pot is preheated to 70-80 DEG C, open and material valve is taken out on homogeneous pot, first take out
Enter 80% water phase components A and oil phase component B, then be pumped into remaining water phase components A flushing pipes, start homogenizer 3min, then open
Cold water stirring is cooled to 65 DEG C, adds component C stirrings, continues cooling and adds component D, stirs to room temperature and deerskin polysaccharide moisturizing is made
Lotion.
2 deerskin polysaccharide moisturizing day cream of Application Example.
Formula:It is shown in Table 2.
2 deerskin polysaccharide moisturizing day cream formula of table.
。
Preparation method:Oil phase component A is first heated with stirring to 80-85 DEG C, insulation 20min sterilizes, then water phase components B is stirred
Mix and be warming up to 85-90 DEG C, insulated sterilizing 15min, homogeneous pot is preheated to 70-80 DEG C, open and material valve is taken out on homogeneous pot, first take out
Enter 80% water phase components A and oil phase component B, then be pumped into remaining water phase components A flushing pipes, start homogenizer 3min, then open
Cold water stirring is cooled to 55 DEG C, adds component C stirrings, continues cooling and adds component D, stirs to room temperature and deerskin polysaccharide moisturizing is made
Day cream.
3 deerskin polysaccharide moisture-keeping crease-shedding of Application Example frost.
Formula:It is shown in Table 3.
3 deerskin polysaccharide moisture-keeping crease-shedding of table frost formula.
。
Preparation method:Oil phase component A is first heated with stirring to 80-85 DEG C, insulation 20min sterilizes, then water phase components B is stirred
Mix and be warming up to 85-90 DEG C, insulated sterilizing 15min, homogeneous pot is preheated to 70-80 DEG C, open and material valve is taken out on homogeneous pot, first take out
Enter 80% water phase components A and oil phase component B, then be pumped into remaining water phase components A flushing pipes, start homogenizer 3min, then open
Cold water stirring is cooled to 55 DEG C, adds component C stirrings, continues cooling and adds component D, stirs to room temperature and deerskin polysaccharide moisturizing is made
Anti-wrinkle cream.
Moisture-keeping cosmetics of the present invention containing deerskin polysaccharide, the specific test method of its moistening effect.
Deerskin polysaccharide moisturizer is as sample, sample 1 in above application embodiment 1-3:Deerskin polysaccharide moisturizing emulsion;Examination
Sample 2:Deerskin polysaccharide moisturizing day cream;Sample 3:Deerskin polysaccharide moisture-keeping crease-shedding frost;Control sample is three kinds of stochastic buying from the market
Moisturizer, control 1 is moisturizing emulsion, control 2 is moisturizing day cream, control 3 is moisture-keeping crease-shedding frost, measures the moisturizing of each product
Rate.
Will be by saturation (NH4)2SO4The drier that aqueous solution prepares relative humidity RH80% is placed in 22 DEG C of isoperibols, will
Medical air-permeable adhesive tape is affixed on 7.0cm × 7.0cm glass plates, and the sample of 0.2000g is evenly coated at and posts breathable adhesive tape
Glass plate on, put into drier and weigh every 4h.Each sample moisturizing experiment is parallel to be repeated 3 times, and takes being averaged for 3 results
Value, calculates moisturizing rate.Its test result is shown in Table 4.
Calculate:Moisturizing rate (%)=M 2/M 1×100%。
In formula:M 2Moisture weight (g) after placement.
M 1Moisture weight (g) before placement.
4 different product of table is in ± 5 moisturizing rate % of RH80% (22 DEG C of room temperature).
。
Result of the test shows:24 when small it is interior containing deerskin polysaccharide cosmetics moisturizing rate between 31.6%-65.9%, compare market
The cosmetics moisturizing rate of upper stochastic buying substantially increases, therefore the cosmetics containing deerskin polysaccharide have fabulous moistening effect.
Cosmetics of the present invention containing deerskin polysaccharide, to skin moisture-keeping and neurodermatitis, contact dermatitis and
Solar dermatitis therapeutic effect.
1st, general information.
Disease:Furfur 30, neurodermatitis 30, contact dermatitis 30, solar dermatitis 30.
Gender:Women 120.
Age:25 years old ~ 30 years old, 10;30 years old ~ 35 years old, 25;35 years old ~ 40 years old, 25;40 years old ~ 45 years old, 30;45 years old
~ 50 years old, 30.
The course for the treatment of:One course for the treatment of cured person 41, effective 53, effective 19, invalid 7.
Normal person's group:Women 30.
2nd, the course for the treatment of and application method.
The course for the treatment of:Surrounding is as a treatment course.
Application method:Deerskin polysaccharide moisturizing emulsion and deerskin polysaccharide moisturizing day cream are 1 time early daily, are uniformly applied to face;Deer
Skin polysaccharide moisturizing emulsion and evening 1 time daily of deerskin polysaccharide moisture-keeping crease-shedding frost, are uniformly applied to face.
3rd, curative effect determinate standard.
Cure:Furfur or inflammatory resolution 90% are with disease symptoms are obviously improved.
It is effective:Furfur or inflammatory resolution 80% ~ 90%, disease symptoms are improved.
Effectively:Furfur or inflammatory resolution 30 ~ 80%, disease symptoms make moderate progress.
It is invalid:Below 30%, disease symptoms improve very little for furfur or inflammatory resolution.
4th, therapeutic effect is shown in Table 5.
5 120 treating skin disease effects of table.
。
5th, normal person's observation the results are shown in Table 6.
6 30 normal persons of table observe result.
。
6th, conclusion.
Deerskin polysaccharide shows a variety of important foster skin skin-protecting functions with its unique molecular structure and physicochemical property, uses it
Make moisturizer, the moisture-keeping functions such as aquation, lubrication can be played, so that skin care, and the factors such as dry skin can be suppressed and caused
The inflammation such as neurodermatitis, contact dermatitis and solar dermatitis.Use the deerskin polysaccharide moisturizing containing active skull cap components
After cosmetics, not only moisture retention and trophism are very notable, but also anti-inflammatory, antibacterial, crease-resistant effect are obvious, fundamentally maintain
The humidity of skin, corrects the symptoms such as dry skin, furfur, inflammation, wrinkle, so as to make skin more water moisten, is delicate, is elastic, is net
In vain, fragile inflammation skin and is improved conscientiously.
7th, model case.
Case one.
Zhang, female, 50 years old, shop-assistant, skin of face was coarse, furfur, other once used moisturizer effects are not notable, used
Deerskin polysaccharide moisture-keeping crease-shedding frost applies outside daily morning and evening, and after a week, furfur symptom all disappears for continuous use, and skin is moist.
Case two.
Dai, female, 41 years old, public transport crew, because of contact dermatitis, flush, was diagnosed as acute contact dermatitis, used
Deerskin polysaccharide moisturizing emulsion applies outside daily morning and evening, and flush symptom improves after two weeks for continuous use.After two months, skin recovers just
Often.
Case three.
Health, female, 29 years old, university teacher, because skin sunburn is seriously dried, face was red and swollen, decortication, is diagnosed as solar lentigines skin
Inflammation, is applied outside daily with deerskin polysaccharide moisturizing day cream, and the continuous use red and swollen disappearance of face after two weeks, skin recovers normal.
Claims (13)
1. application of the deerskin polysaccharide in moisture-keeping cosmetics are prepared, it is characterised in that deerskin is collected, extracts deerskin polysaccharide, and should
It is used to prepare the moisture-keeping cosmetics product for the treatment of neurodermatitis, contact dermatitis and solar dermatitis.
2. application of the deerskin polysaccharide as claimed in claim 1 in moisture-keeping cosmetics are prepared, it is characterised in that to deerskin successively
Lost hair or feathers, cleaned, sterilized, be freeze-dried, crushed, add petroleum ether degreasing.
3. application of the deerskin polysaccharide as claimed in claim 1 in moisture-keeping cosmetics are prepared, it is characterised in that after degreasing
Deerskin powder is using ultrasound wave auxiliary enzyme method extraction deerskin Thick many candies.
4. application of the deerskin polysaccharide as claimed in claim 1 in moisture-keeping cosmetics are prepared, it is characterised in that after enzymolysis
Extracting solution centrifuges, and takes supernatant removing protein.
5. application of the deerskin polysaccharide as claimed in claim 1 in moisture-keeping cosmetics are prepared, it is characterised in that after removing protein
Filtrate dialysis, alcohol precipitation, collect washing after precipitation vacuum freeze drying, obtain deerskin Thick many candies.
6. application of the deerskin polysaccharide as claimed in claim 1 in moisture-keeping cosmetics are prepared, it is characterised in that slightly more to deerskin
Sugar redissolves, and adds quaternary ammonium salt to purify, obtains deerskin refined polysaccharide.
7. application of the deerskin polysaccharide as claimed in claim 1 in moisture-keeping cosmetics are prepared, it is characterised in that will contain after purification
The natural deerskin polysaccharide for having multiple biological activities component is used for the preparation of cosmetics.
8. application of the deerskin polysaccharide as claimed in claim 2 in moisture-keeping cosmetics are prepared, it is characterised in that locate in advance to deerskin
Reason, including lose hair or feathers, clean, sterilize, be freeze-dried, crush, add petroleum ether degreasing;The clean deerskin that specially will first lose hair or feathers is cut
It is less than 0.5cm into granularity2Fragment, sterilize 1-2min, be first placed in quick-frozen 9-10h in -20 DEG C of low temperature refrigerator, then be placed in vacuum
Low temperature frozen dried is carried out in freeze drier, place is crushed with the rotating speed of 12000r/min using high-speed tissue mashing machine after freezing
3-5min is managed, obtains deerskin powder, then adds petroleum ether degreasing, surname extraction 24h, puts room temperature and treat petroleum ether volatilization completely after 4
Saved backup in DEG C refrigerator.
9. application of the deerskin polysaccharide as claimed in claim 3 in moisture-keeping cosmetics are prepared, it is characterised in that after degreasing
Deerskin powder, according to 1:70 solid-liquid ratios add distilled water, surpass under the conditions of ultrasonic power 450-500W, 85-90 DEG C of Extracting temperature
Sound extracts 50-60min, then with 1mol/LNaOH tune pH value to 7.0, the Papain of addition deerskin powder quality 2.5-3.0%
Enzyme, 2.5-3.0% trypsase, 1.0-1.5% alkali proteases and 1.0-1.5% neutral proteinases, in 45-50 DEG C of water bath
Middle enzymolysis 2-3h, is finally placed in 100 DEG C of water-bath high temperature enzyme deactivation 15min by enzymolysis liquid.
10. application of the deerskin polysaccharide as claimed in claim 4 in moisture-keeping cosmetics are prepared, it is characterised in that after enzyme deactivation
Extracting solution removing protein, concrete operations:5-8min is centrifuged with 5000r/min, takes supernatant to be concentrated under reduced pressure into the half of original volume,
By concentrate and solution of trichloroacetic acid(By volume 15:1)It is uniformly mixed, centrifugal filtration after 3h is precipitated in 4 DEG C of environment, collects filter
Liquid.
11. application of the deerskin polysaccharide as claimed in claim 5 in moisture-keeping cosmetics are prepared, it is characterised in that to removing protein
Filtrate afterwards, is initially charged with the bag filter that molecular cut off is 3500Da and uses tap water and distilled water successively each dialysis 24h respectively,
The half of original volume is concentrated under reduced pressure into, adds 95% ethanol precipitation 12h of 4 times of volumes, 15min is centrifuged with 5000 r/min, it is heavy to collect
Form sediment, washed repeatedly with absolute ethyl alcohol 3 times, room temperature is dried, after the quick-frozen 9-10h of -20 DEG C of low temperature refrigerators, in vacuum 0.08-
It is 0.09MPa, dry under the conditions of -85 DEG C, obtain deerskin Thick many candies.
12. application of the deerskin polysaccharide as claimed in claim 6 in moisture-keeping cosmetics are prepared, it is characterised in that thick to deerskin
Polysaccharide adds appropriate distilled water to redissolve, and 3% cetyl trimethylammonium bromide solution is added dropwise until precipitation completely, centrifuges after standing 12h
Filtering, precipitation 2mol/L NaCl redissolve, and add 95% ethanol of 3 times of volumes, precipitation are collected after alcohol precipitation 12h, with appropriate distilled water
Redissolve, is fitted into molecular cut off in the bag filter of 3500Da, successively respectively to be dialysed 24h, being concentrated under reduced pressure with tap water and distilled water
4 times of 95% ethanol precipitation 12h of volume are added after to the half of original volume, collection is deposited in quick-frozen 9-10h in -20 DEG C of low temperature refrigerators,
Vacuum 0.08-0.09MPa will be deposited in, be freeze-dried under the conditions of -85 DEG C, obtain deerskin refined polysaccharide.
13. application of the deerskin polysaccharide as claimed in claim 7 in moisture-keeping cosmetics are prepared, it is characterised in that by weight hundred
Point than for 6.00% deerskin polysaccharide of 1.00%- as main functional component, be used to prepare deerskin polysaccharide moisturizing emulsion, deerskin polysaccharide
The moisture-keeping cosmetics such as moisturizing day cream and deerskin polysaccharide moisture-keeping crease-shedding frost, this serial deerskin polysaccharide moisture-keeping cosmetics not only have excellent
Skin-moisturizing, skin characteristic, but also with crease-resistant, treatment neurodermatitis, contact dermatitis and solar dermatitis and other effects.
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