CN108743431B - Anti-aging and beautifying small-molecule peptide mask and preparation method thereof - Google Patents
Anti-aging and beautifying small-molecule peptide mask and preparation method thereof Download PDFInfo
- Publication number
- CN108743431B CN108743431B CN201810840998.4A CN201810840998A CN108743431B CN 108743431 B CN108743431 B CN 108743431B CN 201810840998 A CN201810840998 A CN 201810840998A CN 108743431 B CN108743431 B CN 108743431B
- Authority
- CN
- China
- Prior art keywords
- mask
- aging
- extract
- beautifying
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0212—Face masks
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Emergency Medicine (AREA)
- Zoology (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses an anti-aging and beautifying small molecular peptide mask and a preparation method thereof, wherein the mask comprises the following components: egg white peptide, soybean polypeptide, pawpaw fermentation liquor, blueberry fermentation liquor, fructus forsythiae extract, periostracum cicada extract, rose extract, lemon essential oil, acetyl hexapeptide-8, vitamin E, hyaluronic acid, oat beta-glucan, menthol, lecithin, hydroxypropyl cellulose, methylparaben, EDTA disodium, nipagin ester and the balance of distilled water. The anti-aging and beautifying small molecular peptide mask provided by the invention is mainly used for resisting aging and removing wrinkles of the face, has the effects of whitening and moisturizing, has the advantages of no toxicity, no stimulation, allergy prevention, wrinkle reduction, cell vitality enhancement, skin elasticity enhancement, fine, smooth and glossy skin, obvious anti-wrinkle and anti-aging effects, no side effect, safety, reliability and allergy resistance, is suitable for various skin types, and can be used as a mask skin care product for a long time.
Description
Technical Field
The invention belongs to the technical field of beauty and skin care, and particularly relates to an anti-aging and beauty-maintaining small-molecule peptide facial mask and a preparation method thereof.
Background
With the improvement of living standard, consumers have higher requirements on skin care products, and particularly in Asia skin care markets, the skin care products for resisting senility and beautifying are most concerned by middle-aged consumers. However, in the existing skin care product market, the anti-aging and beautifying facial mask generally has the defects of single required functional component, poor product stability, poor anti-wrinkle effect, toxic and side effects and the like.
Disclosure of Invention
The invention provides an anti-aging and beautifying small molecular peptide mask and a preparation method thereof. The egg white peptide, the soybean polypeptide and other plant extracts are selected as effective components, and are matched with pawpaw fermentation liquor and blueberry fermentation liquor with an antioxidation effect, so that superoxide radicals and hydroxyl radicals in the skin can be removed, fine wrinkles can be smoothed, the skin can be tightened, meanwhile, deep water can be supplied to the skin, and the skin color can be brightened.
The technical scheme adopted by the invention is as follows:
the anti-aging and beautifying small molecular peptide mask is characterized by comprising the following components in percentage by weight: 3-8% of egg white peptide, 2-6% of soybean polypeptide, 10-20% of pawpaw fermentation liquor, 20-40% of blueberry fermentation liquor, 1.0-3.0% of fructus forsythiae extract, 0.3-1.2% of cicada slough extract, 0.5-1.5% of rose extract, 0.3-1.5% of lemon essential oil, 0.11-0.26% of acetyl 6 peptide-80.05, 0.03-0.30% of vitamin E, 0.03-0.28% of hyaluronic acid, 0.02-0.35% of oat beta-glucan, 0.11-0.26% of menthol, 0.05-0.20% of lecithin, 0.03-0.12% of hydroxypropyl cellulose, 0.003-0.08% of methyl hydroxybenzoate, 0.004-0.020% of EDTA disodium, 0.01-0.03% of nipagin ester, and the balance of distilled water.
Further, the composition preferably comprises the following components in percentage by weight: 4-6% of egg white peptide, 3.6-5.1% of soybean polypeptide, 12-18% of papaya fermentation liquid, 25-35% of blueberry fermentation liquid, 1.4-2.3% of fructus forsythiae extract, 0.5-1.0% of cicada slough extract, 0.8-1.3% of rose extract, 0.6-1.1% of lemon essential oil, 0.14-0.20% of acetyl hexapeptide-80.08, 0.10-0.22% of vitamin E, 0.04-0.18% of hyaluronic acid, 0.2-0.5% of oat beta-glucan, 0.14-0.20% of menthol, 0.07-0.16% of lecithin, 0.06-0.10% of hydroxypropyl cellulose, 0.005-0.05% of methyl hydroxybenzoate, 0.006-0.015% of EDTA disodium, 0.016-0.026% of nipagin ester, and the balance of distilled water.
The preparation method of the egg white peptide comprises the following steps: mixing egg white with distilled water according to the weight ratio of 1: 2-3, adding 0.25-0.50% of trypsin and 0.15-0.40% of alkaline protease according to the total weight of the mixed solution, carrying out enzymolysis for 4-6 hours at 55-60 ℃, and carrying out membrane filtration, concentration and spray drying on the enzymolysis solution to obtain the egg white peptide.
The preparation method of the pawpaw fermentation liquor comprises the following steps: peeling pawpaw, removing pulp, cutting into pieces, adding water with the weight being 3-5 times that of the pawpaw, crushing, putting the obtained solution into a fermentation tank, and placing the solution according to the proportion of 1.0 multiplied by 107～1.0×10×108Inoculating microzyme with the inoculation amount of cfu/mL, fermenting for 24-48 h at 28-32 ℃, filtering and sterilizing the fermented solution to obtain the pawpaw fermentation liquid.
The preparation method of the blueberry fermentation liquor comprises the following steps: cleaning fresh blueberries, directly crushing the washed blueberries, mixing the fresh blueberries with distilled water according to a feed-liquid ratio of 1: 5-8, putting the obtained solution into a fermentation tank, and carrying out fermentation according to a ratio of 1.0 multiplied by 108～1.0×10×109Inoculating microzyme with the inoculation amount of cfu/mL, fermenting for 24-48 h at 28-32 ℃, filtering and sterilizing the fermented solution, and thus obtaining the blueberry fermentation liquid.
The preparation method of the forsythia suspense extract comprises the following steps: crushing fructus forsythiae, mixing with dichloromethane according to the material-liquid ratio of 1: 3-5, then performing supercritical carbon dioxide extraction, and after the supercritical carbon dioxide extraction is finished, performing reduced pressure concentration and freeze drying on a product to obtain the fructus forsythiae extract. The fructus forsythiae extracted by using dichloromethane has high content of effective components, and has strong antioxidant activity and antibacterial effect.
The supercritical carbon dioxide extraction conditions are as follows: the temperature is 55-60 ℃, the pressure is 35-40 MPa, the flow rate of carbon dioxide is 3-4 mL/min, and the extraction time is 3-3.5 h.
The preparation method of the cicada slough extract comprises the following steps: cleaning periostracum cicada, soaking in water with the weight 5-8 times of that of the periostracum cicada for 0.5-1.0 h, heating to 95-100 ℃, decocting for 1-2 h, collecting decoction, concentrating under reduced pressure, and drying to obtain the periostracum cicada extract. Cicada slough is the molting shell of cicada family insect after black locust feathering, and it contains a large amount of chitin, protein, amino acid, etc. cicada slough has effects of dispelling pathogenic wind heat, promoting eruption and relieving sore throat, removing nebula and improving eyesight, dispelling pathogenic wind and relieving spasm, etc., and its decoction component is used for skin care variety, and has antiallergic effect.
The preparation method of the rose extract comprises the following steps: picking fresh roses, soaking the fresh roses in dew of which the weight is 1.5-3 times that of the roses for 2-3 hours, then squeezing juice by using a wall breaking machine, filtering the juice by using five layers of gauze, and concentrating the filtrate to 5-10% of the original weight to obtain the rose extract. The flos Rosae Rugosae pulp contains multiple skin care components, such as quercetin, bitter substances, fat, tannin, vitamins, etc., and has antiaging and skin whitening effects. Especially, after the dew containing the essence of nature is used for juicing and extracting, the beverage has strong moisturizing and nourishing effects.
The invention also provides a preparation method of the anti-aging and beautifying small molecular peptide mask, which comprises the following steps:
s1, adding acetyl hexapeptide-8, vitamin E, hyaluronic acid, oat beta-glucan, menthol, lecithin, hydroxypropyl cellulose, methyl hydroxybenzoate, EDTA disodium and nipagin ester into distilled water, heating to 65-75 ℃, and stirring for reaction until all raw materials are dissolved;
s2, heating to 55-60 ℃, adding the egg white peptide, the soybean polypeptide, the fructus forsythiae extract and the periostracum cicada extract in the formula amount while stirring, and continuing to stir for 20-30 min;
s3, cooling to 25-30 ℃, adding the papaya fermentation liquor, the blueberry fermentation liquor, the rose extract and the lemon essential oil according to the formula ratio, uniformly stirring, and performing microwave sterilization to obtain the nutrient water of the small molecular peptide mask;
s4, soaking the mask cloth in nutrient water for 20-30 min in a sterile environment, and then sealing and packaging the soaked mask cloth to obtain the micromolecule peptide mask with the functions of resisting aging and beautifying.
The mask cloth is one of silk mask, plant fiber mask, carbon black mask, non-woven fabric mask, biological fiber mask and chitosan mask.
In the formula of the anti-aging and beautifying small molecular peptide facial mask, the egg white peptide and the soybean polypeptide are prepared by decomposing proteins in egg white and soybeans into small molecular peptides by using modern enzyme biotechnology engineering, and the small molecular peptides are easy to be absorbed by skin, so that the cell proliferation can be accelerated, the skin metabolism is promoted, and the skin is activated; the small molecular peptide enters the skin to play the role of a moisturizing factor, can lock the moisture of the skin, deeply moistens the skin, and has the functions of tendering the skin, resisting oxidation and delaying senility.
The papaya fermentation liquid in the formula is obtained by fermenting with saccharomycetes, can generate a large amount of papaya mercaptoylase after fermentation, is an active factor with high biological activity, has a large amount of active mercapto (SH) groups on a molecular chain, can effectively remove superoxide radicals and hydroxyl radicals in a human body, and effectively reduces the content of lipid peroxide in skin, so that the aging of body cells is prevented, and the aging process of the skin is delayed; after fermentation, the molecular substances in the nutrient components are decomposed into small molecules, so that the skin can absorb and convert the small molecules more easily; the blueberry is rich in anthocyanin and phenolic acid, is a pure natural anti-aging nutritional supplement and an antioxidant active agent, and the blueberry nutrient substances after fermentation are more easily absorbed by the skin. The lemon essential oil has fresh fragrance, and the limonene in the lemon essential oil has the effects of whitening, astringing and balancing oil secretion, can remove aged and dead cells, brighten skin color, compact capillaries, promote the generation of collagen and lighten melanin.
The acetyl 6 peptide-8 and the hyaluronic acid have higher anti-wrinkle activity. The vitamin E has a good moisturizing function; oat beta-glucan has an activating effect on macrophages in human skin, and can promote the increase of collagen and elastin in the skin, thereby improving the appearance of the skin; the menthol can be matched with the lemon essential oil to improve the sensory feel of the nutrient water on one hand, and on the other hand, the menthol is a transdermal absorbent which can promote the absorption of the nutrient water in the facial mask by the facial skin and deeply moisturize the skin; the stability of the facial mask can be improved by adding auxiliary materials such as lecithin and the like.
According to the formula of the anti-aging and beautifying small molecular peptide mask, egg white peptide, soybean polypeptide, pawpaw fermentation liquor, blueberry fermentation liquor, fructus forsythiae extract, rose hip extract and lemon essential oil all have certain antioxidant activity, and in addition, acetyl hexapeptide-8 and hyaluronic acid anti-wrinkle components in the formula can deeply resist skin aging, and a natural anti-allergic cicada slough extract and menthol capable of promoting transdermal absorption are added in the formula; the components have mutual comprehensive effects, and are beneficial to deeply permeating nutrient water into skin, locking skin moisture, activating skin vitality, improving skin elasticity and resisting skin aging.
The anti-aging and beautifying small molecular peptide mask provided by the invention is mainly used for resisting aging and removing wrinkles of the face, and has the effects of whitening and moisturizing, wherein the egg white peptide and the soybean polypeptide are combined with various natural extracts to act together as effective components, so that the anti-aging and beautifying small molecular peptide mask has the advantages of no toxicity, no stimulation, allergy prevention, wrinkle reduction, cell vitality enhancement, skin elasticity increase, fine, smooth and glossy skin, remarkable anti-wrinkle and anti-aging effects, no side effect, safety, reliability and allergy resistance, is suitable for various skin types, and can be used as a mask skin care product for a long time.
Detailed Description
The present invention will be described in detail with reference to examples.
Example 1
An anti-aging and beautifying small molecular peptide mask comprises the following components in percentage by weight: 5% of egg white peptide, 4.0% of soybean polypeptide, 15% of pawpaw fermentation liquid, 28% of blueberry fermentation liquid, 1.8% of fructus forsythiae extract, 0.7% of periostracum cicada extract, 1.0% of rose extract, 0.9% of lemon essential oil, 80.10% of acetyl hexapeptide, 0.16% of vitamin E, 0.12% of hyaluronic acid, 0.4% of oat beta-glucan, 0.19% of menthol, 0.11% of lecithin, 0.08% of hydroxypropyl cellulose, 0.02% of methylparaben, 0.010% of EDTA disodium, 0.020% of nipagin ester and the balance of distilled water.
The preparation method of the egg white peptide comprises the following steps: mixing egg white with distilled water according to the weight ratio of 1:2, adding 0.25% of trypsin and 0.40% of alkaline protease according to the total amount of the mixed solution, performing enzymolysis at 55 ℃ for 6 hours, and performing membrane filtration, concentration and spray drying on the enzymolysis solution to obtain the egg white peptide.
The preparation method of the pawpaw fermentation liquor comprises the following steps: peeling fructus Chaenomelis, removing pulp, cutting into pieces, adding water 4 times of fructus Chaenomelis weight, pulverizing, placing the obtained solution in fermentation tank, and adding into the fermentation tank at a ratio of 1.0 × 107Inoculating microzyme with the inoculation amount of cfu/mL, fermenting for 48 hours at 28 ℃, filtering and sterilizing the fermented solution to obtain the pawpaw fermentation liquid.
The blueberry fermentation liquorThe preparation method comprises the following steps: cleaning fresh fructus Myrtilli, pulverizing directly, mixing with distilled water at a ratio of 1:7, placing the obtained solution in a fermentation tank at a ratio of 1.0 × 109Inoculating microzyme with the inoculation amount of cfu/mL, fermenting for 48 hours at 28 ℃, filtering and sterilizing the fermented solution, and thus obtaining the blueberry fermentation liquid.
The preparation method of the forsythia suspense extract comprises the following steps: crushing fructus forsythiae, mixing with dichloromethane according to the material-liquid ratio of 1:3, then performing supercritical carbon dioxide extraction, and after the supercritical carbon dioxide extraction is finished, performing reduced pressure concentration and freeze drying on the product to obtain the fructus forsythiae extract. The supercritical carbon dioxide extraction conditions are as follows: the temperature is 55 ℃, the pressure is 35MPa, the flow rate of carbon dioxide is 3mL/min, and the extraction time is 3 h.
The preparation method of the cicada slough extract comprises the following steps: cleaning periostracum Cicadae, soaking in 6 times of water for 0.5h, heating to 100 deg.C, decocting for 1h, collecting decoction, concentrating under reduced pressure, and drying to obtain the periostracum Cicadae extract.
The preparation method of the rose extract comprises the following steps: picking fresh roses, soaking in dew of which the weight is 3 times that of the roses for 3 hours, squeezing juice by using a wall breaking machine, filtering the juice by using five layers of gauze, and concentrating the filtrate to 10% of the original weight to obtain the rose extract.
The preparation method of the anti-aging and beautifying small molecular peptide mask comprises the following steps:
s1, adding acetyl hexapeptide-8, vitamin E, hyaluronic acid, oat beta-glucan, menthol, lecithin, hydroxypropyl cellulose, methyl hydroxybenzoate, EDTA disodium and nipagin ester into distilled water, heating to 75 ℃, and stirring for reaction until all raw materials are dissolved;
s2, heating the mixture to 60 ℃, adding the egg white peptide, the soybean polypeptide, the fructus forsythiae extract and the periostracum cicada extract in the formula amount while stirring, and continuously stirring for 20-30 min;
s3, cooling the temperature to 30 ℃, adding the papaya fermentation liquor, the blueberry fermentation liquor, the rose extract and the lemon essential oil according to the formula ratio, stirring uniformly, and then performing microwave sterilization to obtain the nutrient water of the small molecular peptide mask;
s4, soaking the silk mask cloth in nutrient water for 20-30 min in a sterile environment, and then sealing and packaging the soaked silk mask cloth to obtain the micromolecule peptide mask with the functions of resisting aging and beautifying.
Example 2
An anti-aging and beautifying small molecular peptide mask comprises the following components in percentage by weight: 3% of egg white peptide, 2% of soybean polypeptide, 10% of pawpaw fermentation liquor, 20% of blueberry fermentation liquor, 1.0% of fructus forsythiae extract, 0.5% of periostracum cicada extract, 0.5% of rose extract, 0.7% of lemon essential oil, 0. 80.05% of acetyl hexapeptide, 0.03% of vitamin E, 0.12% of hyaluronic acid, 0.06% of oat beta-glucan, 0.14% of menthol, 0.09% of lecithin, 0.08% of hydroxypropyl cellulose, 0.005% of methylparaben, 0.007% of disodium EDTA, 0.016% of nipagin ester and the balance of distilled water.
The preparation method of the egg white peptide comprises the following steps: mixing egg white with distilled water according to the weight ratio of 1:3, adding 0.50% of trypsin and 0.20% of alkaline protease according to the total amount of the mixed solution, performing enzymolysis at 60 ℃ for 4 hours, and performing membrane filtration, concentration and spray drying on the enzymolysis solution to obtain the egg white peptide.
The preparation method of the pawpaw fermentation liquor comprises the following steps: peeling fructus Chaenomelis, removing pulp, cutting into pieces, adding water 5 times of fructus Chaenomelis weight, pulverizing, placing the obtained solution in fermentation tank, and adding into the fermentation tank at a ratio of 1.0 × 108Inoculating microzyme with the inoculation amount of cfu/mL, fermenting for 24 hours at 32 ℃, filtering and sterilizing the fermented solution to obtain the pawpaw fermentation liquid.
The preparation method of the blueberry fermentation liquor comprises the following steps: cleaning fresh fructus Myrtilli, pulverizing directly, mixing with distilled water at a ratio of 1:8, placing the obtained solution in a fermentation tank at a ratio of 1.0 × 108Inoculating microzyme with the inoculation amount of cfu/mL, fermenting for 24 hours at 32 ℃, filtering and sterilizing the fermented solution, and thus obtaining the blueberry fermentation liquid.
The preparation method of the forsythia suspense extract comprises the following steps: crushing fructus forsythiae, mixing with dichloromethane according to the material-liquid ratio of 1:5, then performing supercritical carbon dioxide extraction, and after the supercritical carbon dioxide extraction is finished, performing reduced pressure concentration and freeze drying on the product to obtain the fructus forsythiae extract. The supercritical carbon dioxide extraction conditions are as follows: the temperature is 55 ℃, the pressure is 35MPa, the flow rate of carbon dioxide is 4mL/min, and the extraction time is 3.5 h.
The preparation method of the cicada slough extract comprises the following steps: cleaning periostracum Cicadae, soaking in 8 times of water for 1.0h, heating to 100 deg.C, decocting for 2h, collecting decoction, concentrating under reduced pressure, and drying to obtain the periostracum Cicadae extract.
The preparation method of the rose extract comprises the following steps: picking fresh roses, soaking in dew 2 times of the weight of the roses for 2.5h, squeezing juice by using a wall breaking machine, filtering the juice by using five layers of gauze, and concentrating the filtrate to 7% of the original weight to obtain the rose extract.
The preparation method of the anti-aging and beautifying small molecular peptide mask comprises the following steps:
s1, adding acetyl hexapeptide-8, vitamin E, hyaluronic acid, oat beta-glucan, menthol, lecithin, hydroxypropyl cellulose, methyl hydroxybenzoate, EDTA disodium and nipagin ester into distilled water, heating to 65 ℃, and stirring for reaction until all raw materials are dissolved;
s2, heating to 55 ℃, adding the egg white peptide, the soybean polypeptide, the fructus forsythiae extract and the periostracum cicada extract in the formula amount while stirring, and continuously stirring for 20-30 min;
s3, cooling to 25 ℃, adding the papaya fermentation liquor, the blueberry fermentation liquor, the rose extract and the lemon essential oil according to the formula ratio, stirring uniformly, and performing microwave sterilization to obtain the nutrient water of the small molecular peptide mask;
s4, soaking the carbon black mask cloth in nutrient water for 20-30 min in a sterile environment, and then sealing and packaging the soaked mask cloth to obtain the micromolecule peptide mask with the functions of resisting aging and beautifying.
Example 3
An anti-aging and beautifying small molecular peptide mask comprises the following components in percentage by weight: 8% of egg white peptide, 6% of soybean polypeptide, 20% of pawpaw fermentation liquor, 40% of blueberry fermentation liquor, 3.0% of fructus forsythiae extract, 1.2% of periostracum cicada extract, 1.5% of rose extract, 1.5% of lemon essential oil, 80.15% of acetyl hexapeptide, 0.30% of vitamin E, 0.28% of hyaluronic acid, 0.35% of oat beta-glucan, 0.26% of menthol, 0.20% of lecithin, 0.12% of hydroxypropyl cellulose, 0.08% of methylparaben, 0.020% of EDTA disodium, 0.03% of nipagin ester and the balance of distilled water.
The preparation method of the egg white peptide comprises the following steps: mixing egg white with distilled water according to the weight ratio of 1:3, adding 0.30% of trypsin and 0.20% of alkaline protease according to the total amount of the mixed solution, performing enzymolysis at 60 ℃ for 4 hours, and performing membrane filtration, concentration and spray drying on the enzymolysis solution to obtain the egg white peptide.
The preparation method of the pawpaw fermentation liquor comprises the following steps: peeling fructus Chaenomelis, removing pulp, cutting into pieces, adding water 5 times of fructus Chaenomelis weight, pulverizing, placing the obtained solution in fermentation tank, and adding into the fermentation tank at a ratio of 1.0 × 107Inoculating microzyme with the inoculation amount of cfu/mL, fermenting for 24 hours at 32 ℃, filtering and sterilizing the fermented solution to obtain the pawpaw fermentation liquid.
The preparation method of the blueberry fermentation liquor comprises the following steps: cleaning fresh fructus Myrtilli, pulverizing directly, mixing with distilled water at a ratio of 1:8, placing the obtained solution in a fermentation tank at a ratio of 1.0 × 108Inoculating microzyme with the inoculation amount of cfu/mL, fermenting for 48 hours at 28 ℃, filtering and sterilizing the fermented solution, and thus obtaining the blueberry fermentation liquid.
The preparation method of the forsythia suspense extract comprises the following steps: crushing fructus forsythiae, mixing with dichloromethane according to the material-liquid ratio of 1:5, then performing supercritical carbon dioxide extraction, and after the supercritical carbon dioxide extraction is finished, performing reduced pressure concentration and freeze drying on the product to obtain the fructus forsythiae extract. The supercritical carbon dioxide extraction conditions are as follows: the temperature is 60 ℃, the pressure is 35MPa, the flow rate of carbon dioxide is 3mL/min, and the extraction time is 3 h.
The preparation method of the cicada slough extract comprises the following steps: cleaning periostracum Cicadae, soaking in 6 times of water for 0.5h, heating to 95 deg.C, decocting for 2h, collecting decoction, concentrating under reduced pressure, and drying to obtain the periostracum Cicadae extract.
The preparation method of the rose extract comprises the following steps: picking fresh roses, soaking in 1.5 times of dew of the rose for 2.5h, squeezing juice by using a wall breaking machine, filtering the juice by using five layers of gauze, and concentrating the filtrate to 8% of the original weight to obtain the rose extract.
The preparation method of the anti-aging and beautifying small molecular peptide mask comprises the following steps:
s1, adding acetyl hexapeptide-8, vitamin E, hyaluronic acid, oat beta-glucan, menthol, lecithin, hydroxypropyl cellulose, methyl hydroxybenzoate, EDTA disodium and nipagin ester into distilled water, heating to 75 ℃, and stirring for reaction until all raw materials are dissolved;
s2, heating to 55 ℃, adding the egg white peptide, the soybean polypeptide, the fructus forsythiae extract and the periostracum cicada extract in the formula amount while stirring, and continuously stirring for 20-30 min;
s3, cooling to 25 ℃, adding the papaya fermentation liquor, the blueberry fermentation liquor, the rose extract and the lemon essential oil according to the formula ratio, stirring uniformly, and performing microwave sterilization to obtain the nutrient water of the small molecular peptide mask;
s4, soaking the non-woven fabric mask cloth in nutrient water for 20-30 min in a sterile environment, and then sealing and packaging the soaked mask cloth to obtain the micromolecule peptide mask with the functions of resisting aging and beautifying.
Example 4
Evaluation of Using Effect
300 female volunteers aged 30-45 years are randomly selected to evaluate the using effect of the small molecular peptide mask cloth, each group comprises 100, and the using method comprises the following steps: the face is cleaned before sleeping and then applied for 20min, the face is applied once every two days for 30 days continuously, and then the evaluation result is counted. 98% of the testers react to increase the elasticity and the glossiness of the skin after using the mask disclosed by the invention, the mask has obvious whitening and anti-wrinkle effects, and the moisture of the skin after using the mask each time can be increased by more than 100% compared with the skin before using the mask through a moisture meter test, so that the mask has an obvious moisturizing effect.
The above detailed description of the anti-aging and skin-caring small-molecule peptide mask and the preparation method thereof with reference to the examples is illustrative and not restrictive, and several examples can be cited according to the limited scope, so that changes and modifications without departing from the general concept of the present invention shall fall within the protection scope of the present invention.
Claims (9)
1. The anti-aging and beautifying small molecular peptide mask is characterized by comprising the following components in percentage by weight: 3-8% of egg white peptide, 2-6% of soybean polypeptide, 10-20% of pawpaw fermentation liquor, 20-40% of blueberry fermentation liquor, 1.0-3.0% of fructus forsythiae extract, 0.3-1.2% of cicada slough extract, 0.5-1.5% of rose extract, 0.3-1.5% of lemon essential oil, 0.11-0.26% of acetyl hexapeptide-80.05, 0.03-0.30% of vitamin E, 0.03-0.28% of hyaluronic acid, 0.02-0.35% of oat beta-glucan, 0.11-0.26% of menthol, 0.05-0.20% of lecithin, 0.03-0.12% of hydroxypropyl cellulose, 0.003-0.08% of methyl hydroxybenzoate, 0.004-0.020% of EDTA disodium, 0.01-0.03% of nipagin ester, and the balance of distilled water.
2. The anti-aging and beautifying small molecule peptide mask as claimed in claim 1, wherein the preparation method of the papaya fermentation liquor comprises the following steps: peeling pawpaw, removing pulp, cutting into pieces, adding water with the weight being 3-5 times that of the pawpaw, crushing, putting the obtained solution into a fermentation tank, and placing the solution according to the proportion of 1.0 multiplied by 107~1.0×109Inoculating microzyme with the inoculation amount of cfu/mL, fermenting for 24-48 h at 28-32 ℃, filtering and sterilizing the fermented solution to obtain the pawpaw fermentation liquid.
3. The anti-aging and beautifying small molecule peptide mask as claimed in claim 1, wherein the preparation method of the blueberry fermentation liquid comprises the following steps: cleaning fresh blueberries, directly crushing the washed blueberries, mixing the fresh blueberries with distilled water according to a feed-liquid ratio of 1: 5-8, putting the obtained solution into a fermentation tank, and carrying out fermentation according to a ratio of 1.0 multiplied by 108~1.0×1010Inoculating microzyme with the inoculation amount of cfu/mL, fermenting for 24-48 h at 28-32 ℃, filtering and sterilizing the fermented solution, and thus obtaining the blueberry fermentation liquid.
4. The anti-aging and beautifying small molecule peptide mask as claimed in claim 1, wherein the preparation method of the forsythia suspense extract comprises the following steps: crushing fructus forsythiae, mixing with dichloromethane according to the material-liquid ratio of 1: 3-5, then performing supercritical carbon dioxide extraction, and after the supercritical carbon dioxide extraction is finished, performing reduced pressure concentration and freeze drying on a product to obtain the fructus forsythiae extract.
5. The anti-aging and beautifying small molecule peptide mask as claimed in claim 4, wherein the supercritical carbon dioxide extraction conditions are as follows: the temperature is 55-60 ℃, the pressure is 35-40 MPa, the flow rate of carbon dioxide is 3-4 mL/min, and the extraction time is 3-3.5 h.
6. The anti-aging and beautifying small molecule peptide mask as claimed in claim 1, wherein the preparation method of the cicada slough extract comprises the following steps: cleaning periostracum cicada, soaking in water with the weight 5-8 times of that of the periostracum cicada for 0.5-1.0 h, heating to 95-100 ℃, decocting for 1-2 h, collecting decoction, concentrating under reduced pressure, and drying to obtain the periostracum cicada extract.
7. The anti-aging and beautifying small-molecule peptide mask as claimed in claim 1, wherein the preparation method of the rose extract comprises the following steps: picking fresh roses, soaking the fresh roses in dew of which the weight is 1.5-3 times that of the roses for 2-3 hours, then squeezing juice by using a wall breaking machine, filtering the juice by using five layers of gauze, and concentrating the filtrate to 5-10% of the original weight to obtain the rose extract.
8. The preparation method of the anti-aging and beautifying small molecule peptide mask as claimed in any one of claims 1 to 7, is characterized by comprising the following steps:
s1, adding acetyl hexapeptide-8, vitamin E, hyaluronic acid, oat beta-glucan, menthol, lecithin, hydroxypropyl cellulose, methyl hydroxybenzoate, EDTA disodium and nipagin ester into distilled water, heating to 65-75 ℃, and stirring to react until all raw materials are dissolved;
s2, cooling the temperature to 55-60 ℃, adding the egg white peptide, the soybean polypeptide, the fructus forsythiae extract and the periostracum cicadae extract with the formula amount while stirring, and continuing to stir for 20-30 min;
s3, cooling to 25-30 ℃, adding the papaya fermentation liquor, the blueberry fermentation liquor, the rose extract and the lemon essential oil according to the formula ratio, uniformly stirring, and performing microwave sterilization to obtain the nutrient water of the small molecular peptide mask;
s4, soaking the mask cloth in nutrient water for 20-30 min in a sterile environment, and then sealing and packaging the soaked mask cloth to obtain the anti-aging and beautifying small molecule peptide mask.
9. The preparation method according to claim 8, wherein the mask cloth is one of a silk mask, a plant fiber mask, a carbon black mask, a non-woven fabric mask, a bio-fiber mask and a chitosan mask.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810840998.4A CN108743431B (en) | 2018-07-27 | 2018-07-27 | Anti-aging and beautifying small-molecule peptide mask and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810840998.4A CN108743431B (en) | 2018-07-27 | 2018-07-27 | Anti-aging and beautifying small-molecule peptide mask and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108743431A CN108743431A (en) | 2018-11-06 |
| CN108743431B true CN108743431B (en) | 2021-05-14 |
Family
ID=63971993
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810840998.4A Active CN108743431B (en) | 2018-07-27 | 2018-07-27 | Anti-aging and beautifying small-molecule peptide mask and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108743431B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110522662A (en) * | 2019-08-29 | 2019-12-03 | 福州东星生物技术有限公司 | A kind of ganoderma lucidum face cream and preparation method thereof |
| TW202114631A (en) * | 2019-10-07 | 2021-04-16 | 大江生醫股份有限公司 | Fermentation broth of carica papaya and uses thereof for beautifying skin |
| CN111728910A (en) * | 2020-07-03 | 2020-10-02 | 广州无添加主义化妆品有限公司 | Fermented extract of blueberry and snow lotus herb, preparation method and application thereof |
| CN112716864B (en) * | 2021-03-03 | 2022-12-16 | 山东博科医用材料有限公司 | Rose whitening and moisturizing mask |
-
2018
- 2018-07-27 CN CN201810840998.4A patent/CN108743431B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| 東北膜樣五穀豐登系列大豆倍嫩保濕面膜;无;《http://www.cosdna.com/cht/cosmetic_b89b226148.html》;20160321;第1页 * |
| 蛋清肽的抗氧化稳定性与功能特性;牛慧慧等;《食品科学》;20111231;第32卷(第15期);第139-143页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108743431A (en) | 2018-11-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108653035B (en) | Whitening and moisturizing small-molecule peptide mask and preparation method thereof | |
| CN108743431B (en) | Anti-aging and beautifying small-molecule peptide mask and preparation method thereof | |
| CN111574618B (en) | Preparation method of whitening compound protein peptidase hydrolysate, whitening compound protein peptide beverage and preparation method thereof | |
| CN104523490B (en) | A kind of exfoliating composition with skin whitening, moisturizing effect and preparation method thereof | |
| CN103356446B (en) | A kind of natural anti-aging skin care product containing black tea extract and preparation method thereof | |
| CN107802543B (en) | Roxburgh rose element skin-refreshing biological fiber mask and preparation method thereof | |
| CN103690469A (en) | Skin moisturizer containing ginkgo enzyme, as well as preparation method and application of skin moisturizer | |
| CN111643411B (en) | Anti-aging composition and application thereof | |
| CN109939064B (en) | Essence containing probiotic fermentation metabolite and application of essence in cosmetics | |
| CN110507582A (en) | A kind of anti-ageing remediation composition and its preparation method and application containing composite vegetables extractive | |
| CN108309862A (en) | Pudilan whitening moisturizing face masque | |
| KR20120087451A (en) | Compositions for Improving Skin Conditions | |
| CN107095820A (en) | A kind of preparation method of saururus chinensis whitening emulsion | |
| CN108888554B (en) | Stem cell beauty emulsion with skin tendering and spot lightening functions and preparation method thereof | |
| CN114343092A (en) | Collagen compound beverage and preparation method and application thereof | |
| CN113616571A (en) | Cream water containing schizosaccharomyces cerevisiae for skin repair and preparation method thereof | |
| CN107397716A (en) | Promote cell turnover and can compact fair-skinned white product and preparation method | |
| CN110680775A (en) | Pure whitening, moisturizing and hydrating liquid and preparation method thereof | |
| CN109303734A (en) | A kind of perfume Flos Nelumbinis extract and its preparing the application in cosmetics | |
| CN107951817A (en) | A kind of whitening wrinkle-removing facial mask of ferment containing mirabalan and preparation method | |
| CN113975203A (en) | Emulsion added with camellia oleifera flower extract and preparation process thereof | |
| CN108179054B (en) | Extraction method of American hickory seed oil and application of American hickory seed oil in hair dye | |
| CN106107964A (en) | Ginseng ferment and preparation method thereof | |
| KR102441142B1 (en) | Method for preparing ginseng extract, ginseng extract and cosmetic composition for anti-aging of skin comprising the same | |
| CN102028631A (en) | Natural bioactive factor additive for daily cosmetics |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220321 Address after: 570100 Tsinghua maker (Hainan) incubation base, No. 7, Keji Avenue, national high tech Industrial Development Zone, Haikou City, Hainan Province Patentee after: Guoke precision (Hainan) Medical Technology Co.,Ltd. Address before: Room 1718, block a, Greenland Yinghai international building, 760 Ma'anshan South Road, Baohe District, Hefei City, Anhui Province 230000 Patentee before: ANHUI PRECISION MEDICAL INDUSTRY RESEARCH INSTITUTE Co.,Ltd. |