CN105432329B - Improve the cultural method of Queensland nut graft survival rate - Google Patents

Improve the cultural method of Queensland nut graft survival rate Download PDF

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CN105432329B
CN105432329B CN201510866888.1A CN201510866888A CN105432329B CN 105432329 B CN105432329 B CN 105432329B CN 201510866888 A CN201510866888 A CN 201510866888A CN 105432329 B CN105432329 B CN 105432329B
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grafting
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queensland nut
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CN105432329A (en
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凌华彪
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Guangxi Heshan Runhong Agricultural Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/26Phosphorus; Compounds thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action

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Abstract

The invention discloses a kind of cultural method for improving Queensland nut graft survival rate, comprise the following steps:The cultivation of stock:After rootstock seedling grows 57 leaves, there is the notch of 3cm long from stock face is mitered into top at sand bed matrix top layer 8cm, the borehole in the middle part of its notch, forms the blind cylindrical hole with 1.5cm long;The processing of scion:Fringe bar is cut into bevel cuts to base portion at its base portion 3cm, and 1cm long and the cylindrical protrusions of the blind cylindrical hole matching of rootstock seedling are reserved in the middle part of bevel cuts, its bevel cuts is directed at and cylindrical protrusions smears protective agent;First grafting:It is added dropwise in the blind cylindrical hole of rootstock seedling and promotees grafting dilution agent liquid, the cylindrical protrusions of scion are aligned to the blind cylindrical hole of rootstock seedling, and spraying promotees grafting dilution agent liquid on the inclined-plane of its docking, rootstock seedling is combined closely with scion and completes just grafting.The method of grafting cultivation Queensland nut of the present invention, graft survival rate are up to 91.7%.

Description

Improve the cultural method of Queensland nut graft survival rate
Technical field
The present invention relates to the cultivation field of Queensland nut.It is more particularly related to a kind of Queensland nut that improves is transferred It is connected into the cultural method of motility rate.
Background technology
Queensland nut, also known as Queensland's chestnut, macadamia.Its benevolence is rich in unrighted acid, protein, vitamin High Deng, nutritive value, flavor is very unique, is known as the title of " king of dry fruit ".Queensland nut market prospects are had an optimistic view of, ecological benefits, Economic benefit tool is good, is a kind of high, full of nutrition, unique flavor the edible nut of oil content, is constantly in the international market State that supply falls short of demand.
The defects of Queensland nut breeding generally uses engrafting method, is primarily present two aspects:On the one hand, traditional grafting Method survival rate is low, general only 50% or so, as soon as a worker daily about can 200 plants of grafting, the estimation half that can live is pretty good; On the other hand, the Queensland nut graft seedling growth cycle is grown, and traditional raising seedling of Australian nut method is worked as at the liftoff 20-30cm of stock, When stem slightly reaches 0.7-1.2cm, grafting can be carried out, meets the stock of this condition, at least to grow more than 12 months, is managed In the case of good, garden can be gone out within 6 months after grafting, at least need the 1.5-3 years from being seeded into Cloned seedling and going out garden.Thus, Grafting fruit shoot survival percent how is improved, shortens grafting growing-seedling period, the development to Queensland nut planting industry seems very urgent It is and important.
The content of the invention
It is an object of the invention to solve at least the above, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of cultural method for improving Queensland nut graft survival rate, the present invention carries A kind of protective agent has been supplied, timely protection is done for the scion cut, has been strengthened after scion is cut from maternal plant to the life before grafting Power, so as to improve the graft survival rate of scion.
Promote grafting dilution agent liquid it is a still further object of the present invention to provide one kind and promote grafting gel, respectively in first grafting The blind cylindrical hole and first grafting for being added drop-wise to rootstock seedling are coated at grafting after 7-8 days, substantially increase graft survival rate.
In order to realize these purposes and further advantage according to the present invention, there is provided one kind improves Queensland nut grafting survival The cultural method of rate, comprises the following steps:
Step A, the cultivation of stock:
Queensland nut seed is placed in the first soak before step 1) sowing 4-6h is soaked at 25-30 DEG C, remove drift The seed on liquid level is floated over, remaining seed clear water is fished for and rinses and be placed on and be mounted with the constant temperature oscillator of the second soak, is shaken Immersion 15-20h is swung, it is 40-45 DEG C to control the second soak temperature, and kind must be soaked by taking out, wherein the first soak is quality point Number is the trisodium phosphate aqueous solution of 0.5%-0.8%, and the second soak is red mould by 24-32 parts by weight chitosan, 15-20 parts by weight Element, 30-40 parts by weight egg vinegar liquids and 1000 parts by weight of deionized water are made;
Immersion kind is placed in be equipped with the culturing rack of one layer of flax fiber cloth bottom hollow out and tiled by step 2), and in seed Upper cap on two layers of flax fiber cloth, be placed in temperature as 35-38 DEG C, vernalization in the incubator that relative humidity is 80%-85% Culture, it is water-soluble for the bamboo vinegar of 0.8%-1.2% that mass fraction spray directly on two layers of flax fiber cloth at interval of 6h Liquid, until the rudiment hole width of seed season crack takes out when reaching more than 2.5mm;
Step 3) mid or late October beats kind of a cave in sand bed matrix, in a manner of the seed in step 2) is put by rudiment hole side It is put in kind of cave, after rootstock seedling grows 5-7 piece leaves, stock face is mitered into top tool from sand bed matrix top layer 8cm There are the notch of 3cm long, and the borehole in the middle part of its notch, form the blind cylindrical hole with 1.5cm long;
Step B, the processing of scion:
Macadamia is selected to give birth to semi-lignified, the branch of health then as fringe bar, during collection, clip length reaches 8- The fringe bar of 10cm long, and bevel cuts are cut to base portion at its base portion 3cm, and reserve in the middle part of bevel cuts 1cm long with The cylindrical protrusions that the blind cylindrical hole of rootstock seedling matches, are directed at its bevel cuts and cylindrical protrusions smear protective agent, stand-by, Protectant preparation method is as follows:The aloe barbadensis Miller of 30-42 parts by weight is smashed to pieces, 20 mesh sieve cloth is crossed and obtains jelly, will The dandelion of 3-5 parts by weight and the radix glycyrrhizae of 3-4 parts by weight add the grinding of 10-15 parts by weight of deionized water after crushing, with jelly It is uniformly mixed, adds the konjaku flour of 1-2 parts by weight and the deionized water of 50-60 parts by weight, after mixing up to the protection Agent;
Step C, first grafting:
It is added dropwise in the blind cylindrical hole for the rootstock seedling that step 3) obtains and promotees grafting dilution agent liquid, every plant of 3 drop of rootstock seedling drop, By the blind cylindrical hole of the cylindrical protrusions alignment rootstock seedling of the obtained scion of step B, and sprayed on the inclined-plane of its docking Promote grafting dilution agent liquid, rootstock seedling is combined closely with scion and complete just grafting, the rush grafting dilution agent liquid is by 4-8 parts by weight Chitosan, 10-14 parts by weight Sambucus thunbergiana, 5-8 part by weight of vitamin A, 3-5 parts by weight α-naphthylacetic acid, 40-60 parts by weight mulberries ferment Element and 500-600 parts by weight of deionized water are made.
Preferably, the cultural method of described raising Queensland nut graft survival rate, after first grafting 7-8 days, in grafting Place coating promote grafting gel, it is described rush grafting gel be 15-18 parts by weight mulberry enzyme, 20-25 parts by weight aloe barbadensis Miller, 0.8-1.1 parts by weight Corn element, 12-15 parts by weight Sambucus thunbergiana, 25-28 parts by weight konjaku powder and 200 parts by weight water are made, its Specifically preparation method is:By the Sambucus thunbergiana crushed after being dried of above-mentioned parts by weight into powder, mixed with the konjaku powder of above-mentioned parts by weight Close uniformly, add the water of above-mentioned parts by weight, after stirring evenly, obtain the first mixture;Again by the aloe barbadensis Miller of above-mentioned parts by weight After smashing to pieces, 20 mesh sieve cloth are crossed, are mixed with the mulberry enzyme and zeatin of above-mentioned parts by weight, then be uniformly mixed with the first mixture, Up to the rush grafting gel.
Preferably, the cultural method of the raising Queensland nut graft survival rate, wherein the sand bed matrix includes Two layers, upper strata is 3 according to mass ratio by the completely decomposed shuck of thick river sand and warp:1 composition, upper thickness 12-15cm, under It is 6 that layer, which is bog moss, thin river sand and passes through completely decomposed mushroom bacteria residue according to mass ratio,:2:3 compositions, lower thickness 18- 24cm。
Preferably, the cultural method of the raising Queensland nut graft survival rate, the scion of grafting is Guangxi kind The hot No. 1 Queensland nut kind in the osmanthus of plant, rootstock seedling are No. 695 Queensland nut kinds of Guangxi plantation.
Preferably, the cultural method of the raising Queensland nut graft survival rate, after first grafting 12-14 days, Quarter is hindered 2-3 in interval at the 4-5cm of lower part at rootstock seedling grafting.
Preferably, the cultural method of described raising Queensland nut graft survival rate, it is to be grafted survive after, remove stock Rudiment on seedling, and the unnecessary branch in scion is trimmed, stay a major branch to continue to cultivate, scion takes out the tip more than 3 times, and blade two It is secondary it is aging after can go out garden field planting.
The present invention includes at least following beneficial effect:
1) present invention is in the cultivating process of rootstock seedling, by Queensland nut seed elder generation weak base immersion treatment, corrosion kind Skin, promotes seed water suction, to screen out the seed of water absorbing capacity force difference, then by weakly acidic second soak vibrates immersion treatment, Sterilize to it, and allow it fully to absorb water and ensure moisture of germinating needs, the condition that abundance is provided is sprouted for seed preparation, is promoted Into the sprouting of rootstock seedling, shorten the cultivation time of rootstock seedling;
2) immersion kind culture coats seed up and down using flax fiber cloth, it can be promoted to absorb battalion to when opening germinal aperature The moisture in water and culture environment is supported, while completely cuts off the germ of exterior introducing, under conditions of nutritive water is applied in interval leaching, rapidly Natural cracking goes out germinal aperature, wherein, the bamboo vinegar in nutritive water has antibacterial, promotes Seed Germination and growth effect;
3) protective agent is smeared after scion is cut from maternal plant can keep the vitality of scion, improve surviving for scion grafting Quercitrin-3-O-glucoside in rate, wherein dandelion, the chalcone in radix glycyrrhizae have antibacterial activity;Konjaku flour water suction is swollen Swollen coefficient is big, can be used as moisturizer;Aloe barbadensis Miller is plant doctor, it contains tinctura aloes, is the very strong material of antibiotic property, It can kill the germs such as fungi, mould, bacterium, virus, suppress and the development of eliminating pathogen is bred, contain bradykinin enzyme and more Sugar, can improve the resistance to germ, also be acted on insect prevention;
4) scion is grafted onto on rootstock seedling by the mode of the blind cylindrical hole of the cylindrical protrusions alignment rootstock seedling of scion, Be conducive to scion grafting survival, promoting the chitosan in grafting dilution agent liquid has antibiotic property, and Sambucus thunbergiana and vitamin A, which have, to be promoted Merged between scion and stock, promote the effect of grafting survival;α-naphthylacetic acid is plant growth regulator, can acute activation plant Immune system, promotes cell division and expands, and boosts metabolism and photosynthesis, promotion wound healing improve surviving for grafting Rate;Mulberry enzyme strengthens its vitality with the resistance of enhancing scion and stock to pathogen, promotion cell metabolism Effect, promotes grafting survival;Promoting the aloe barbadensis Miller in grafting gel and konjaku powder has antibacterial, water conservation, improves seed life The effect of power is ordered, zeatin has the function that to promote callus healing.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this The research and practice of invention and be understood by the person skilled in the art.
Embodiment
With reference to specific embodiment, the present invention is described in further detail, to make those skilled in the art's reference say Bright book word can be implemented according to this.
It should be noted that experimental method described in following embodiments, is conventional method unless otherwise specified, institute Reagent and material are stated, unless otherwise specified, is commercially obtained.
Embodiment 1:
A kind of cultural method for improving Queensland nut graft survival rate, comprises the following steps:
Step A, the cultivation of stock:
Queensland nut seed is placed in the first soak before step 1) sowing 4h is soaked at 25 DEG C, removing swims in liquid Seed on face, fishes for remaining seed clear water and rinses and be placed on and be mounted with the constant temperature oscillator of the second soak, vibration immersion 15h, it is 40 DEG C to control the second soak temperature, and kind must be soaked by taking out, wherein the phosphorus that it is 0.5% that the first soak, which is mass fraction, Sour three sodium water solutions, the second soak is by 24 parts by weight chitosans, 15 parts by weight gibberellin, 30 parts by weight egg vinegar liquids and 1000 weights Amount part deionized water is made;
Immersion kind is placed in be equipped with the culturing rack of one layer of flax fiber cloth bottom hollow out and tiled by step 2), and in seed Upper cap on two layers of flax fiber cloth, be placed in temperature as 35 DEG C, vernalization culture in the incubator that relative humidity is 80%, every The bamboo vinegar aqueous solution that a mass fraction is 0.8% is sprayed directly on two layers of flax fiber cloth every 6h, until seed is natural The rudiment hole width to split takes out when reaching more than 2.5mm;
Step 3) mid or late October beats kind of a cave in sand bed matrix, in a manner of the seed in step 2) is put by rudiment hole side It is put in kind of cave, adds permeable, after rootstock seedling grows 5-7 piece leaves, is mitered into stock face from sand bed matrix top layer 8cm Top has the notch of 3cm long, and the borehole in the middle part of its notch, forms the blind cylindrical hole with 1.5cm long;
Step B, the processing of scion:
Macadamia is selected to give birth to semi-lignified, the branch of health then as fringe bar, during collection, clip length reaches 8cm Long fringe bar, and bevel cuts are cut to base portion at its base portion 3cm, and 1cm long and stock are reserved in the middle part of bevel cuts The cylindrical protrusions that the blind cylindrical hole of seedling matches, are directed at its bevel cuts and cylindrical protrusions smear protective agent, stand-by, described Protectant preparation method is as follows:The aloe barbadensis Miller of 30 parts by weight is smashed to pieces, 20 mesh sieve cloth is crossed and obtains jelly, by 3 parts by weight Dandelion and 3 parts by weight radix glycyrrhizae crush after add 10 parts by weight of deionized water grinding, be uniformly mixed with jelly, add 1 The deionized water of the konjaku flour of parts by weight and 50 parts by weight, after mixing up to the protective agent;
Step C, first grafting:
It is added dropwise in the blind cylindrical hole for the rootstock seedling that step 3) obtains and promotees grafting dilution agent liquid, every plant of 3 drop of rootstock seedling drop, By the blind cylindrical hole of the cylindrical protrusions alignment rootstock seedling of the obtained scion of step B, and sprayed on the inclined-plane of its docking Promote grafting dilution agent liquid, rootstock seedling is combined closely with scion and complete just grafting, the rush grafting dilution agent liquid is by 4 parts by weight shells Glycan, 10 parts by weight Sambucus thunbergianas, 5 part by weight of vitamin A, 3 parts by weight α-naphthylacetic acids, 40 parts by weight mulberry enzymes and 500 weight Part deionized water is made.
Wherein, the cultural method of described raising Queensland nut graft survival rate, after first grafting 7 days, coats at grafting Promote grafting gel, the rush grafting gel is 15 parts by weight mulberry enzymes, 20 parts by weight aloe barbadensis Millers, 0.8 parts by weight Corn Element, 12 parts by weight Sambucus thunbergianas, 25 parts by weight konjaku powders and 200 parts by weight water are made, its specific preparation method is:Will be above-mentioned heavy The Sambucus thunbergiana crushed after being dried of part is measured into powder, is uniformly mixed with the konjaku powder of above-mentioned parts by weight, adds above-mentioned parts by weight Water, after stirring evenly, obtains the first mixture;After the aloe barbadensis Miller of above-mentioned parts by weight is smashed to pieces again, 20 mesh sieve cloth are crossed, it is and above-mentioned Mulberry enzyme and the zeatin mixing of parts by weight, then be uniformly mixed with the first mixture, up to the rush grafting gel.
Wherein, the cultural method of the raising Queensland nut graft survival rate, wherein the sand bed matrix includes two layers, Upper strata is 3 according to mass ratio by the completely decomposed shuck of thick river sand and warp:1 composition, upper thickness 12cm, lower floor is peat Moss, thin river sand and the complete decomposed mushroom bacteria residue of warp are 6 according to mass ratio:2:3 compositions, lower thickness 18cm.
Wherein, the cultural method of the raising Queensland nut graft survival rate, after first grafting 12 days, is transferred in rootstock seedling Connect interval at place lower part 4cm and carve wound 2.
Wherein, the cultural method of described raising Queensland nut graft survival rate, it is to be grafted survive after, remove on rootstock seedling Rudiment, and trim the unnecessary branch in scion, stay a major branch to continue to cultivate, scion takes out the tip more than 3 times, and blade is secondary old Garden field planting can be gone out after ripe.
Embodiment 2:
A kind of cultural method for improving Queensland nut graft survival rate, comprises the following steps:
Step A, the cultivation of stock:
Queensland nut seed is placed in the first soak before step 1) sowing 5h is soaked at 28 DEG C, removing swims in liquid Seed on face, fishes for remaining seed clear water and rinses and be placed on and be mounted with the constant temperature oscillator of the second soak, vibration immersion 18h, it is 43 DEG C to control the second soak temperature, and kind must be soaked by taking out, wherein it is 0.65% that the first soak, which is mass fraction, Trisodium phosphate aqueous solution, the second soak is by 28 parts by weight chitosans, 18 parts by weight gibberellin, 35 parts by weight egg vinegar liquids and 1000 Parts by weight of deionized water is made;
Immersion kind is placed in be equipped with the culturing rack of one layer of flax fiber cloth bottom hollow out and tiled by step 2), and in seed Upper cap on two layers of flax fiber cloth, be placed in temperature as 36 DEG C, vernalization culture in the incubator that relative humidity is 83%, every The bamboo vinegar aqueous solution that a mass fraction is 1.0% is sprayed directly on two layers of flax fiber cloth every 6h, until seed is natural The rudiment hole width to split takes out when reaching more than 2.5mm;
Step 3) mid or late October beats kind of a cave in sand bed matrix, in a manner of the seed in step 2) is put by rudiment hole side It is put in kind of cave, adds permeable, after rootstock seedling grows 6 leaves, is mitered into stock face from sand bed matrix top layer 8cm Portion has the notch of 3cm long, and the borehole in the middle part of its notch, forms the blind cylindrical hole with 1.5cm long;
Step B, the processing of scion:
Macadamia is selected to give birth to semi-lignified, the branch of health then as fringe bar, during collection, clip length reaches 9cm Long fringe bar, and bevel cuts are cut to base portion at its base portion 3cm, and 1cm long and stock are reserved in the middle part of bevel cuts The cylindrical protrusions that the blind cylindrical hole of seedling matches, are directed at its bevel cuts and cylindrical protrusions smear protective agent, stand-by, described Protectant preparation method is as follows:The aloe barbadensis Miller of 36 parts by weight is smashed to pieces, 20 mesh sieve cloth is crossed and obtains jelly, by 4 parts by weight Dandelion and 3.5 parts by weight radix glycyrrhizae crush after add 13 parts by weight of deionized water grinding, be uniformly mixed with jelly, add The deionized water of the konjaku flour of 1.5 parts by weight and 55 parts by weight, after mixing up to the protective agent;
Step C, first grafting:
It is added dropwise in the blind cylindrical hole for the rootstock seedling that step 3) obtains and promotees grafting dilution agent liquid, every plant of 3 drop of rootstock seedling drop, By the blind cylindrical hole of the cylindrical protrusions alignment rootstock seedling of the obtained scion of step B, and sprayed on the inclined-plane of its docking Promote grafting dilution agent liquid, rootstock seedling is combined closely with scion and complete just grafting, the rush grafting dilution agent liquid is by 6 parts by weight shells Glycan, 12 parts by weight Sambucus thunbergianas, 6 part by weight of vitamin A, 4 parts by weight α-naphthylacetic acids, 50 parts by weight mulberry enzymes and 550 weight Part deionized water is made.
Wherein, the cultural method of described raising Queensland nut graft survival rate, after first grafting 8 days, coats at grafting Promote grafting gel, the rush grafting gel is 16 parts by weight mulberry enzymes, 23 parts by weight aloe barbadensis Millers, 0.9 parts by weight Corn Element, 13 parts by weight Sambucus thunbergianas, 26 parts by weight konjaku powders and 200 parts by weight water are made, its specific preparation method is:Will be above-mentioned heavy The Sambucus thunbergiana crushed after being dried of part is measured into powder, is uniformly mixed with the konjaku powder of above-mentioned parts by weight, adds above-mentioned parts by weight Water, after stirring evenly, obtains the first mixture;After the aloe barbadensis Miller of above-mentioned parts by weight is smashed to pieces again, 20 mesh sieve cloth are crossed, it is and above-mentioned Mulberry enzyme and the zeatin mixing of parts by weight, then be uniformly mixed with the first mixture, up to the rush grafting gel.
Wherein, the cultural method of the raising Queensland nut graft survival rate, wherein the sand bed matrix includes two layers, Upper strata is 3 according to mass ratio by the completely decomposed shuck of thick river sand and warp:1 composition, upper thickness 13cm, lower floor is peat Moss, thin river sand and the complete decomposed mushroom bacteria residue of warp are 6 according to mass ratio:2:3 compositions, lower thickness 20cm.
Wherein, the cultural method of the raising Queensland nut graft survival rate, the scion of grafting is what Guangxi was planted Osmanthus No. 1 Queensland nut kind of heat, rootstock seedling are No. 695 Queensland nut kinds of Guangxi plantation.
Wherein, the cultural method of the raising Queensland nut graft survival rate, after first grafting 13 days, is transferred in rootstock seedling Connect interval at place lower part 5cm and carve wound 3.
Wherein, the cultural method of described raising Queensland nut graft survival rate, it is to be grafted survive after, remove on rootstock seedling Rudiment, and trim the unnecessary branch in scion, stay a major branch to continue to cultivate, scion takes out the tip more than 3 times, and blade is secondary old Garden field planting can be gone out after ripe.
Embodiment 3:
A kind of cultural method for improving Queensland nut graft survival rate, comprises the following steps:
Step A, the cultivation of stock:
Queensland nut seed is placed in the first soak before step 1) sowing 6h is soaked at 30 DEG C, removing swims in liquid Seed on face, fishes for remaining seed clear water and rinses and be placed on and be mounted with the constant temperature oscillator of the second soak, vibration immersion 20h, it is 45 DEG C to control the second soak temperature, and kind must be soaked by taking out, wherein the phosphorus that it is 0.8% that the first soak, which is mass fraction, Sour three sodium water solutions, the second soak is by 32 parts by weight chitosans, 20 parts by weight gibberellin, 40 parts by weight egg vinegar liquids and 1000 weights Amount part deionized water is made;
Immersion kind is placed in be equipped with the culturing rack of one layer of flax fiber cloth bottom hollow out and tiled by step 2), and in seed Upper cap on two layers of flax fiber cloth, be placed in temperature as 38 DEG C, vernalization culture in the incubator that relative humidity is 85%, every The bamboo vinegar aqueous solution that a mass fraction is 1.2% is sprayed directly on two layers of flax fiber cloth every 6h, until seed is natural The rudiment hole width to split takes out when reaching more than 2.5mm;
Step 3) mid or late October beats kind of a cave in sand bed matrix, in a manner of the seed in step 2) is put by rudiment hole side It is put in kind of cave, adds permeable, after rootstock seedling grows 5-7 piece leaves, is mitered into stock face from sand bed matrix top layer 8cm Top has the notch of 3cm long, and the borehole in the middle part of its notch, forms the blind cylindrical hole with 1.5cm long;
Step B, the processing of scion:
Macadamia is selected to give birth to semi-lignified, the branch of health then as fringe bar, during collection, clip length reaches 10cm Long fringe bar, and bevel cuts are cut to base portion at its base portion 3cm, and 1cm long and stock are reserved in the middle part of bevel cuts The cylindrical protrusions that the blind cylindrical hole of seedling matches, are directed at its bevel cuts and cylindrical protrusions smear protective agent, stand-by, described Protectant preparation method is as follows:The aloe barbadensis Miller of 42 parts by weight is smashed to pieces, 20 mesh sieve cloth is crossed and obtains jelly, by 5 parts by weight Dandelion and 4 parts by weight radix glycyrrhizae crush after add 15 parts by weight of deionized water grinding, be uniformly mixed with jelly, add 2 The deionized water of the konjaku flour of parts by weight and 60 parts by weight, after mixing up to the protective agent;
Step C, first grafting:
It is added dropwise in the blind cylindrical hole for the rootstock seedling that step 3) obtains and promotees grafting dilution agent liquid, every plant of 3 drop of rootstock seedling drop, By the blind cylindrical hole of the cylindrical protrusions alignment rootstock seedling of the obtained scion of step B, and sprayed on the inclined-plane of its docking Promote grafting dilution agent liquid, rootstock seedling is combined closely with scion and complete just grafting, the rush grafting dilution agent liquid is by 8 parts by weight shells Glycan, 14 parts by weight Sambucus thunbergianas, 8 part by weight of vitamin A, 5 parts by weight α-naphthylacetic acids, 60 parts by weight mulberry enzymes and 600 weight Part deionized water is made.
Wherein, the cultural method of described raising Queensland nut graft survival rate, after first grafting 8 days, coats at grafting Promote grafting gel, the rush grafting gel is 18 parts by weight mulberry enzymes, 25 parts by weight aloe barbadensis Millers, 1.1 parts by weight Corn Element, 15 parts by weight Sambucus thunbergianas, 28 parts by weight konjaku powders and 200 parts by weight water are made, its specific preparation method is:Will be above-mentioned heavy The Sambucus thunbergiana crushed after being dried of part is measured into powder, is uniformly mixed with the konjaku powder of above-mentioned parts by weight, adds above-mentioned parts by weight Water, after stirring evenly, obtains the first mixture;After the aloe barbadensis Miller of above-mentioned parts by weight is smashed to pieces again, 20 mesh sieve cloth are crossed, it is and above-mentioned Mulberry enzyme and the zeatin mixing of parts by weight, then be uniformly mixed with the first mixture, up to the rush grafting gel.
Wherein, the cultural method of the raising Queensland nut graft survival rate, wherein the sand bed matrix includes two layers, Upper strata is 3 according to mass ratio by the completely decomposed shuck of thick river sand and warp:1 composition, upper thickness 12-15cm, lower floor are Bog moss, thin river sand and the complete decomposed mushroom bacteria residue of warp are 6 according to mass ratio:2:3 compositions, lower thickness 24cm.
Wherein, the cultural method of the raising Queensland nut graft survival rate, the scion of grafting is what Guangxi was planted Osmanthus No. 1 Queensland nut kind of heat, rootstock seedling are No. 695 Queensland nut kinds of Guangxi plantation.
Wherein, the cultural method of the raising Queensland nut graft survival rate, after first grafting 14 days, is transferred in rootstock seedling Connect interval at place lower part 5cm and carve wound 3.
Wherein, the cultural method of described raising Queensland nut graft survival rate, it is to be grafted survive after, remove on rootstock seedling Rudiment, and trim the unnecessary branch in scion, stay a major branch to continue to cultivate, scion takes out the tip more than 3 times, and blade is secondary old Garden field planting can be gone out after ripe.
Comparative example 1:
On the basis of embodiment 2, using common inclined-plane patterning method, transferred after stock and scion are sheared in a manner of chamferring Connect, without using the rush grafting dilution agent liquid in embodiment 2, also without using promoting grafting gel, other methods with embodiment 2 unanimously into Row is cultivated, under the cultural method for drawing comparative example 1, the survival rate only 72% of Queensland nut grafting, and grafting engrafting and cultivating 6 It can just go out garden after month, and the survival rate of the Queensland nut grafting of embodiment 2 is up to 91.7%, grafting engrafting and cultivating 3 months With regard to garden can be gone out.
Comparative example 2:
On the basis of embodiment 2, the method for removal " after first grafting 8 days, the coating rush grafting gel at grafting ", other Cultural method is identical with embodiment 2, and under the cultural method for drawing comparative example 2, the survival rate only 84% of Queensland nut grafting, is transferred Seedlings picking engrafting and cultivating can just go out garden after 5 months.
Comparative example 3:
On the basis of embodiment 2, the sand bed matrix replaced in embodiment 2 is common rough sand river matrix, other cultivations Method is identical with embodiment 2, and under the cultural method for drawing comparative example 3, the survival rate of Queensland nut grafting has 89%, but grafting Seedling engrafting and cultivating can just go out garden after 6 months.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art Realize other modification, therefore under the universal limited without departing substantially from claim and equivalency range, it is of the invention and unlimited In specific details and shown here as the embodiment with description.

Claims (6)

1. a kind of cultural method for improving Queensland nut graft survival rate, it is characterised in that comprise the following steps:
Step A, the cultivation of stock:
Queensland nut seed is placed in the first soak before step 1) sowing 4-6h is soaked at 25-30 DEG C, removing is swum in Seed on liquid level, fishes for remaining seed clear water and rinses and be placed on and be mounted with the constant temperature oscillator of the second soak, vibration leaching 15-20h is steeped, control the second soak temperature, kind must be soaked by taking out for 40-45 DEG C, wherein the first soak is mass fraction is The trisodium phosphate aqueous solution of 0.5%-0.8%, the second soak by 24-32 parts by weight chitosan, 15-20 parts by weight gibberellin, 30-40 parts by weight egg vinegar liquids and 1000 parts by weight of deionized water are made;
Immersion kind is placed in be equipped with the culturing rack of one layer of flax fiber cloth bottom hollow out and tiled by step 2), and in the upper of seed Portion covers two layers of flax fiber cloth, is placed in temperature as 35-38 DEG C, vernalization culture in the incubator that relative humidity is 80%-85%, The bamboo vinegar aqueous solution that a mass fraction is 0.8%-1.2% is sprayed directly on two layers of flax fiber cloth at interval of 6h, directly Taken out when reaching more than 2.5mm to the rudiment hole width of seed season crack;
Step 3) mid or late October beats kind of a cave in sand bed matrix, and the seed in step 2) is put in a manner of rudiment hole side is put In kind cave, after rootstock seedling grows 5-7 piece leaves, there is 3cm from stock face is mitered into top at sand bed matrix top layer 8cm Long notch, and the borehole in the middle part of its notch, form the blind cylindrical hole with 1.5cm long;
Step B, the processing of scion:
Macadamia is selected to give birth to semi-lignified, the branch of health then as fringe bar, during collection, clip length reaches 8-10cm long Fringe bar, and cut bevel cuts to base portion at its base portion 3cm, and 1cm long and rootstock seedling are reserved in the middle part of bevel cuts The cylindrical protrusions that match of blind cylindrical hole, be directed at its bevel cuts and cylindrical protrusions and smear protective agent, stand-by, the guarantor The preparation method for protecting agent is as follows:The aloe barbadensis Miller of 30-42 parts by weight is smashed to pieces, 20 mesh sieve cloth is crossed and obtains jelly, by 3-5 weight The dandelion of part and the radix glycyrrhizae of 3-4 parts by weight add the grinding of 10-15 parts by weight of deionized water after crushing, mixed with jelly It is even, the konjaku flour of 1-2 parts by weight and the deionized water of 50-60 parts by weight are added, after mixing up to the protective agent;
Step C, first grafting:
It is added dropwise in the blind cylindrical hole for the rootstock seedling that step 3) obtains and promotees grafting dilution agent liquid, every plant of 3 drop of rootstock seedling drop, will walks The blind cylindrical hole of the cylindrical protrusions alignment rootstock seedling for the scion that rapid B is obtained, and spray rush on the inclined-plane of its docking and transfer Dilution agent liquid is connect, rootstock seedling is combined closely with scion and completes just grafting, the rush grafting dilution agent liquid is gathered by 4-8 parts by weight shells Sugar, 10-14 parts by weight Sambucus thunbergiana, 5-8 part by weight of vitamin A, 3-5 parts by weight α-naphthylacetic acid, 40-60 parts by weight mulberry enzyme and 500-600 parts by weight of deionized water is made.
2. the cultural method of Queensland nut graft survival rate is improved as claimed in claim 1, it is characterised in that first grafting 7-8 After it, the coating rush grafting gel at grafting, the rush grafting gel is 15-18 parts by weight mulberry enzyme, 20-25 parts by weight storehouse Drag-line aloe, 0.8-1.1 parts by weight Corn element, 12-15 parts by weight Sambucus thunbergiana, 25-28 parts by weight konjaku powder and 200 parts by weight Water is made, its specific preparation method is:By the Sambucus thunbergiana crushed after being dried of above-mentioned parts by weight into powder, the evil spirit with above-mentioned parts by weight Taro fine powder is uniformly mixed, and adds the water of above-mentioned parts by weight, after stirring evenly, obtains the first mixture;Again by the storehouse of above-mentioned parts by weight After drag-line aloe is smashed to pieces, 20 mesh sieve cloth are crossed, are mixed with the mulberry enzyme and zeatin of above-mentioned parts by weight, then mixed with the first mixture Close uniformly, up to the rush grafting gel.
3. the cultural method of Queensland nut graft survival rate is improved as claimed in claim 2, it is characterised in that wherein described sand Bed substrate includes two layers, and upper strata is 3 according to mass ratio by the completely decomposed shuck of thick river sand and warp:1 composition, upper thickness are 12-15cm, it is 6 that lower floor, which is bog moss, thin river sand and passes through completely decomposed mushroom bacteria residue according to mass ratio,:2:3 compositions, lower thickness Spend for 18-24cm.
4. the cultural method of Queensland nut graft survival rate is improved as claimed in claim 1, it is characterised in that grafting connects Fringe is the hot No. 1 Queensland nut kind in osmanthus of Guangxi plantation, and rootstock seedling is No. 695 Queensland nut kinds of Guangxi plantation.
5. the cultural method of Queensland nut graft survival rate is improved as claimed in claim 3, it is characterised in that in first grafting After 12-14 days, hinder 2-3 quarter being spaced at rootstock seedling grafting at the 4-5cm of lower part.
6. the cultural method of Queensland nut graft survival rate is improved as claimed in claim 5, it is characterised in that to be grafted to survive Afterwards, remove the rudiment on rootstock seedling, and trim the unnecessary branch in scion, stay a major branch to continue to cultivate, scion take out the tip 3 times with On, and blade it is secondary it is aging after can go out garden field planting.
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CN105961110A (en) * 2016-05-05 2016-09-28 广西南亚热带农业科学研究所 Method for improving yield and quality of macadamia nut
CN106973704A (en) * 2017-03-27 2017-07-25 西双版纳云濮农业科技有限公司 A kind of engrafting method of Queensland nut
CN107494158A (en) * 2017-09-28 2017-12-22 马山县荷松种植专业合作社 Improve the engrafting method of Queensland nut fruit quality
CN107810730A (en) * 2017-09-28 2018-03-20 马山县荷松种植专业合作社 The engrafting method of Queensland nut
CN108093908A (en) * 2017-11-14 2018-06-01 广西博白县乾福真家庭农场 The cultural method of Queensland nut
CN110249824B (en) * 2019-07-17 2021-03-26 广西南亚热带农业科学研究所 Method for repairing injured macadamia nut trees
CN110393119A (en) * 2019-07-31 2019-11-01 广西澳一澳农业科技有限公司 A kind of Queensland nut seed fast seedling growing propagation method
CN110999729A (en) * 2019-12-26 2020-04-14 湖南素里养农业科技有限公司 Crop seed cultivation method
CN111406526B (en) * 2020-04-21 2022-04-15 广西壮族自治区蚕业技术推广站 High-position grafting rapid propagation method for mulberry
CN112400508A (en) * 2020-12-07 2021-02-26 贵州省核桃研究所 Method for grafting walnut on wild walnut stock with high survival rate

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CN104025844A (en) * 2014-05-24 2014-09-10 云南源润坚果开发有限公司 Macadimia nut seedling autumn grafting technology
CN104272983B (en) * 2014-10-16 2016-06-08 山西桃运扁桃技术开发研究所 A kind of almond budding method
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CN104920078A (en) * 2015-06-10 2015-09-23 岑溪市新丰农业综合开发有限公司 Method for quickly rearing macadamia nut grafted seedlings through plantlets

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