CN107810730A - The engrafting method of Queensland nut - Google Patents
The engrafting method of Queensland nut Download PDFInfo
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- CN107810730A CN107810730A CN201710899673.9A CN201710899673A CN107810730A CN 107810730 A CN107810730 A CN 107810730A CN 201710899673 A CN201710899673 A CN 201710899673A CN 107810730 A CN107810730 A CN 107810730A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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Abstract
The invention discloses a kind of engrafting method of Queensland nut, comprise the following steps：Step 1: immersion treatment is carried out to seed；Step 2: immersion kind is placed in into vernalization culture in incubator, illumination is alternately irradiated with irradiation, after seedling grows to 10~15cm, is moved into greenhouse and is cultivated；Step 3: after the seedling in greenhouse grows 6~8 leaves, select stem thick and be used as grafting stock for 1~1.5cm seedling shearing；Step 4: selecting fringe bar, and the fringe bar chamfer is put into nutrient solution and soaked, taking-up, which is put into incubator, stores, be standby；Step 5: spraying graft union agent on the bevel cuts of stock and the bevel cuts of fringe bar, and the bevel cuts of fringe bar are directed to the bevel cuts of stock, bundled stock and fringe bar with film bandage, grafting is completed.The high graft survival rate of the present invention, nursery stage are short, the fruit quality of Queensland nut after final grafting is excellent.
Description
Technical field
The present invention relates to the cultivation field of Queensland nut, specifically a kind of engrafting method of Queensland nut.
Background technology
Queensland nut is also known as macadamia, originates in Australia, is the five-star edible nut in the world, have " nut it
King ", the good reputation of " the optimal edible nut in the world ".China starts commodity in the later stage 1980s and plants Australia heavily fortified point
Fruit, it is concentrated mainly on Yunnan and Guangxi.Queensland nut remarkable in economical benefits is planted, is rare suitable China torrid areas
The specialty economies seeds of plantation.
Grafting is one of method of vegetative propagation, that is, takes the branch on improved seeds plant or bud to be connected to the suitable of another plant
Work as position, make both with reference to and generate new plant.The branch or bud connected is called scion or buddered, and connects with scion or budder
Plant be stock.
Using the new plant of propagation by grafiting the merit and can of its maternal plant can be kept to utilize the advantageous feature of stock
(such as：Resistance, adaptability, early fruiting character etc.).Engrafting method, the quality of stock, quality of fringe bar etc. are to fruit product after grafting
The quality of matter has an impact.Engrafting method of the prior art for Queensland nut inadequate science on concrete operation step, leads
Cause graft survival rate is relatively low, is unfavorable for large area to plant.
The content of the invention
It is an object of the invention to solve at least the above, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of engrafting method of Queensland nut, high graft survival rate, nursery stage be short,
It is adapted to large area plantation.
In order to realize according to object of the present invention and further advantage, there is provided a kind of engrafting method of Queensland nut,
Comprise the following steps：
Step 1: Queensland nut seed is placed in into 4~6h of immersion in clear water, the seed of floating is removed, fishes for remaining seed
8~10h of vibration immersion in soak is placed in, the temperature for controlling soak is 35~40 DEG C, and kind must be soaked by taking out, wherein, immersion
Liquid is by the tertiary sodium phosphate of 17~22 parts by weight, the chitosan of 18~25 parts by weight, the gibberellin of 6~12 parts by weight, 2~3 weight
The deionized water that the sodium selenite, the spirit hair element of 4~7 parts by weight of part are dissolved in 1000 parts by weight is made；
Step 2: immersion kind is placed in into vernalization culture in incubator, cultivation temperature is 25 DEG C, culture humidity is 70%~
80%, light application time summation is 8h/ days, exposure time summation is 80min/ days, and illumination treats that seedling is grown with irradiating alternately
To after 10~15cm, move into greenhouse and cultivate, wherein, multigroup irradiation fluorescent tube, every group of irradiation fluorescent tube bag are oppositely arranged in incubator
Include the feux rouges fluorescent tube that gold-tinted fluorescent tube, a wavelength that a wavelength is 590nm are 810nm and the purple light that a wavelength is 370nm
Fluorescent tube, and a feux rouges fluorescent tube and a ultraviolet lamp tube are arranged side by side directly over a gold-tinted fluorescent tube, illumination replaces with irradiation
The specific method of progress is：First carry out 1h illumination, carry out 10min irradiation again；
Step 3: after the seedling in greenhouse grows 6~8 leaves, the thick seedling for 1~1.5cm of stem is selected from apart from it
At the 12cm of top cut, expose smooth section, as grafting stock, then from the section 3cm of stock towards section
Oblique partial application, after beveling, the bevel cuts angle at 45 ° of stock；
Step 4: from macadamia it is raw then, it is semi-lignified on the sunny side on branch sheared length be 11~12cm, stem it is thick
Branch for 1~1.5cm, with 1~2 leaf bud is chamfer as fringe bar, and from sheared edge 3cm towards sheared edge, tiltedly
After cutting, the bevel cuts of fringe bar and the angle of its sheared edge are 45 °, and then the fringe bar chamfer is put into nutrient solution and soaks 3
After~5min, taking-up, which is put into incubator, stores, is standby, wherein, filtrated air is passed through in incubator, controls the temperature in incubator
Spend for 13~15 DEG C, humidity is 85%~90%, and nutrient solution includes following raw material：The Nafusaku of 6~9 parts by weight, 2~7 weights
Measure the gibberellin of part, the asparagus juice of 2~5 parts by weight, the keel extract solution of 4~7 parts by weight, the root of Dahurain angelica of 5~8 parts by weight, 1~2
Ground blood perfume, the Pu'er tea ferment of 13~17 parts by weight, the vitamin C of 7~12 parts by weight, the konjaku of 4~5 parts by weight of parts by weight
Powder；
Step 5: spray graft union agent on the bevel cuts of stock and the bevel cuts of fringe bar, and by fringe bar
Bevel cuts are directed at the bevel cuts of stock, are bundled stock and fringe bar with film bandage, and grafting is completed, wherein, the ring of grafting
Border temperature is 24~28 DEG C, and graft union agent includes the raw material of following parts by weight：2~3 parts of vitamin A, 2.5~3.5 parts
Eupatorium adenophorum, 0.5~1 part of lipoic acid, 3~6 parts of heteroauxin, 4~5 parts of polyvinyl alcohol, 1~2 part of olive polyphenol,
5~7 parts of propolis, 0.2~0.8 part of potassium citrate, 2~2.5 parts of chitosan, 0.4~0.9 part of garlic selenium polysaccharide, 0.2
~0.3 part of capric acid.
Preferably, wherein, the irradiation of the 10min in step 2 irradiates for 5 circulations, and irradiation every time first carries out 115s
Simple gold-tinted fluorescent tube irradiation, the combination irradiation of the rear feux rouges fluorescent tube for carrying out 5s and ultraviolet lamp tube.
Preferably, the incubator described in step 2 includes：
Casing, it is cube structure, and one of side is can be with the chamber door of folding, at left and right sides of the chamber door
Side inner walls respectively between the upper and lower away from two parallel projections are provided with along the cabinet depth direction, the raised length is less than
Side where it is divided into three regions by the depth of the casing, two projections of any side, and each region is equal
Along raised bearing of trend between the upper and lower away from setting gradually multigroup irradiation fluorescent tube, on the inwall of the chamber door and side corresponding thereto
The LED plant lamps that two away from setting power are 45W between the upper and lower respectively；
Two culture plates, it is cube structure, wherein two relative sides cave inward to be formed and the projection
The groove being adapted, so that the culture plate is interval with along the raised slip, the culture tray bottom from edge to center
Multi-turn air-vent, the rectangle that is shaped as of air-vent, and its cornerwise intersection point and the center superposition of the culture tray bottom are often enclosed,
The bottom of the culture plate is made up of transparency silica glass；
Wherein, the LED plant lamps set above any one of culture plate on the cabinet wall with it is described
Circumference of the fluorescent tube along the casing is irradiated to form a circle.
Preferably, the projection of the incubator is provided with cylindrical locating dowel, the positioning close to the end face of the chamber door
Post is provided with screw thread, and the culture plate is respectively equipped with integrally formed with the side towards the opposite end of the side of the chamber door
Fixed plate, the fixed plate are provided with positioning hole, wherein, it is described after the culture plate is slipped into the incubator along its groove
Locating dowel is through the positioning hole, be spirally connected fixation by nut.
Preferably, laying depth ratio is 1 to the culture tray bottom successively:4 flax fiber layer of cloth and growth substrate layer,
Wherein, growth substrate layer by the fine earth of 6~8 parts by weight, the vermiculite power of 15~20 parts by weight, 12~16 parts by weight porous pottery
Grain, the macadamia shell powder of the sawdust of 11~14 parts by weight, 8~10 parts by weight are mixed.
Preferably, step 2 infiltrates flax fiber cloth water-soluble in the bamboo vinegar that mass fraction is 0.4%~0.6%
Liquid, taking-up are screwed onto not draining, are layed in the culture tray bottom, then the basic unit of laying growth above it.
Preferably, the film bandage in step 5 is bandage made of polytetrafluoroethylene film or biological sealed membrane.
The present invention comprises at least following beneficial effect：
The immersion kind of Queensland nut is put into incubator by the present invention, is replaced by LED plant lamps with multigroup irradiation fluorescent tube
Illumination and irradiation are carried out, and the bottom of culture plate is transparent quartz glass, is available for the light of fluorescent tube to pass through, and is sprouted in seed
During, batch (-type) mutagenic treatment is carried out to seed using gold-tinted and red, purple light, the time of seed germination is shortened, anvil is made
It is beneficial to grafting, survival rate height after wood, the fruit quality of the Queensland nut after final grafting is excellent.
The present invention makees the fringe bar of grafting using the branch of semi-lignified, and its degree of lignification is low, and histocyte is active, can
It is quick to form callus, shorten the graft seedling growth phase, improve graft survival rate.
The present invention in incubator set can pull culture plate, when needing seedling being transplanted into greenhouse, will can train
The seedling supported disk and be grown in it is extracted out together, is delivered in greenhouse, a seedling can be transplanted at once when being taken out from culture plate
Enter in the soil in greenhouse, the root system of seedling is stayed in soil to greatest extent, maintain the fresh and alive of seedling, improve survival rate, drop
Low grafting cost, avoid make because of transplanting seedling root system it is prolonged it is exposed wilt outside, after transplanting it is even dead
Phenomenon.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Brief description of the drawings
Fig. 1 is the overall structure diagram of incubator of the present invention；
Fig. 2 is the partial structural diagram of incubator of the present invention；
Fig. 3 is the structural representation of culture plate of the present invention.
Embodiment
The present invention is described in further detail with reference to the accompanying drawings and examples, to make those skilled in the art's reference
Specification word can be implemented according to this.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of individual other elements or its combination.It should be noted that experimental method described in following embodiments, such as without spy
Different explanation, is conventional method, the reagent and material, unless otherwise specified, commercially obtains；The present invention's
In description, term " transverse direction ", " longitudinal direction ", " axial direction ", " radial direction ", " on ", " under ", "front", "rear", "left", "right", " vertical ",
The orientation or position relationship of the instruction such as " level ", " top ", " bottom ", " interior ", " outer " are to be closed based on orientation shown in the drawings or position
System, it is for only for ease of and describes of the invention and simplify description, is not that the device of instruction or hint meaning or element must have
Specific orientation, with specific azimuth configuration and operation, therefore be not considered as limiting the invention.
<Embodiment 1>
A kind of engrafting method of Queensland nut of the present invention, comprises the following steps：
4h is soaked Step 1: Queensland nut seed is placed in clear water, removes the seed of floating, remaining seed is fished for and is placed in
Vibration immersion 8h in soak, the temperature for controlling soak is 35 DEG C, and kind must be soaked by taking out, wherein, soak is by 17 parts by weight
Tertiary sodium phosphate, the chitosan of 18 parts by weight, the gibberellin of 6 parts by weight, sodium selenite, the spirit hair element of 4 parts by weight of 2 parts by weight
The deionized water for being dissolved in 1000 parts by weight is made；
Step 2: immersion kind is placed in into vernalization culture in incubator, cultivation temperature is 25 DEG C, and culture humidity is 70%, light
Be 8h/ days according to temporal summation, exposure time summation be 80min/ days, and illumination with irradiation alternately, treat that seedling grows to 10cm
Afterwards, move into greenhouse and cultivate, wherein, multigroup irradiation fluorescent tube is oppositely arranged in incubator, every group of irradiation fluorescent tube includes a wavelength
The ultraviolet lamp tube that the feux rouges fluorescent tube and a wavelength for being 810nm for 590nm gold-tinted fluorescent tube, a wavelength are 370nm, and one
A feux rouges fluorescent tube and a ultraviolet lamp tube, specific side of the illumination with irradiation alternately are arranged side by side directly over gold-tinted fluorescent tube
Method is：First carry out 1h illumination, carry out 10min irradiation again；
Wherein, 10min irradiation is that 5 circulations irradiate, and irradiation every time first carries out 115s simple gold-tinted fluorescent tube spoke
Irradiated according to, the combination of the rear feux rouges fluorescent tube for carrying out 5s and ultraviolet lamp tube；
Step 3: after the seedling in greenhouse grows 6 leaves, the thick seedling for 1~1.5cm of stem is selected from apart from its top
Cut at 12cm, expose smooth section, chamfer as grafting stock, then from the section 3cm of stock towards section
One knife, after beveling, the bevel cuts angle at 45 ° of stock；
Step 4: from macadamia it is raw then, it is semi-lignified on the sunny side on branch sheared length be 11~12cm, stem it is thick
Branch for 1~1.5cm, with 1~2 leaf bud is chamfer as fringe bar, and from sheared edge 3cm towards sheared edge, tiltedly
After cutting, the bevel cuts of fringe bar and the angle of its sheared edge are 45 °, and then the fringe bar chamfer is put into nutrient solution and soaked
After 3min, taking-up, which is put into incubator, stores, is standby, wherein, filtrated air is passed through in incubator, controls the temperature in incubator
For 13 DEG C, humidity 85%, nutrient solution includes following raw material：The Nafusaku of 6 parts by weight, the gibberellin of 2 parts by weight, 2 weight
Asparagus juice, the keel extract solution of 4 parts by weight, the root of Dahurain angelica of 5 parts by weight, the ground blood perfume, the Pu'er tea of 13 parts by weight of 1 parts by weight of part
Ferment, the vitamin C of 7 parts by weight, the konjaku flour of 4 parts by weight；
Step 5: spray graft union agent on the bevel cuts of stock and the bevel cuts of fringe bar, and by fringe bar
Bevel cuts are directed at the bevel cuts of stock, are bundled stock and fringe bar with film bandage, and grafting is completed, wherein, the ring of grafting
Border temperature is 24 DEG C, and graft union agent includes the raw material of following parts by weight：2 parts of vitamin A, 2.5 parts of Eupatorium adenophorum, 0.5
The lipoic acid, 3 parts of heteroauxin, 4 parts of polyvinyl alcohol, 1 part of olive polyphenol, 5 parts of propolis, 0.2 part of citric acid of part
Potassium, 2 parts of chitosan, 0.4 part of garlic selenium polysaccharide, 0.2 part of capric acid.
Wherein, the incubator described in step 2 includes：
Casing 1, it is cube structure, and one of side is can be left positioned at the chamber door 11 with the chamber door 11 of folding
The side inner walls of right both sides along the depth direction of casing 1 respectively between the upper and lower away from being provided with two parallel projections 12, the projection
12 length is less than the depth of the casing 1, and the side where it is divided into three by two described raised 12 of any side
Region, each region along projection 12 bearing of trend between the upper and lower away from set gradually it is multigroup irradiation fluorescent tube 13, the He of chamber door 11
The LED plant lamps 14 that two away from setting power are 45W between the upper and lower respectively on the inwall of side corresponding thereto；
Two culture plates 2, it is cube structure, wherein two relative sides cave inward to be formed and the projection
12 grooves 22 being adapted, so that the culture plate 2 slides along described raised 12, the bottom of culture plate 2 is from edge to center
Multi-turn air-vent 23 is interval with, often encloses the rectangle that is shaped as of air-vent 23, and its cornerwise intersection point and the bottom of culture plate 2
The center superposition in portion, the bottom of the culture plate 2 are made up of transparency silica glass；
Wherein, the LED plant lamps 14 that the top of any one of culture plate 2 is set on the inwall of casing 1 with
Circumference of the irradiation fluorescent tube 13 along the casing 1 forms a circle.
The projection 12 of the incubator is provided with cylindrical locating dowel 121, the locating dowel close to the end face of the chamber door 11
121 are provided with screw thread, the culture plate 2 towards the opposite end of the side of the chamber door 11 be respectively equipped with side one into
The fixed plate of type, the fixed plate are provided with positioning hole 221, wherein, the culture plate 2 slips into the incubator along its groove 22
After interior, the locating dowel 121 is through the positioning hole 221, be spirally connected fixation by nut.
Laying depth ratio is 1 successively for the bottom of culture plate 2:4 flax fiber layer of cloth and growth substrate layer, wherein, it is raw
Long hypothallus is by the fine earth of 6 parts by weight, the vermiculite power of 15 parts by weight, the porous ceramic grain of 12 parts by weight, the sawdust of 11 parts by weight, 8
The macadamia shell powder of parts by weight is mixed.
Step 2 infiltrates flax fiber cloth is screwed onto not draining in the bamboo vinegar aqueous solution that mass fraction is 0.4%, taking-up,
It is layed in the culture tray bottom, then the basic unit of laying growth above it.
Film bandage in step 5 is bandage made of polytetrafluoroethylene film or biological sealed membrane.
<Embodiment 2>
A kind of engrafting method of Queensland nut of the present invention, comprises the following steps：
5h is soaked Step 1: Queensland nut seed is placed in clear water, removes the seed of floating, remaining seed is fished for and is placed in
Vibration immersion 9h in soak, the temperature for controlling soak is 38 DEG C, and kind must be soaked by taking out, wherein, soak is by 20 parts by weight
Tertiary sodium phosphate, the chitosan of 22 parts by weight, the gibberellin of 9 parts by weight, sodium selenite, the spirit hair element of 5 parts by weight of 3 parts by weight
The deionized water for being dissolved in 1000 parts by weight is made；
Step 2: immersion kind is placed in into vernalization culture in incubator, cultivation temperature is 25 DEG C, and culture humidity is 75%, light
Be 8h/ days according to temporal summation, exposure time summation be 80min/ days, and illumination with irradiation alternately, treat that seedling grows to 13cm
Afterwards, move into greenhouse and cultivate, wherein, multigroup irradiation fluorescent tube 13 is oppositely arranged in incubator, every group of irradiation fluorescent tube 13 includes one
The ultraviolet lamp tube that the feux rouges fluorescent tube and a wavelength that gold-tinted fluorescent tube that wavelength is 590nm, a wavelength are 810nm are 370nm, and
A feux rouges fluorescent tube and a ultraviolet lamp tube, tool of the illumination with irradiation alternately are arranged side by side directly over one gold-tinted fluorescent tube
Body method is：First carry out 1h illumination, carry out 10min irradiation again；
Wherein, 10min irradiation is that 5 circulations irradiate, and irradiation every time first carries out 115s simple gold-tinted fluorescent tube spoke
Irradiated according to, the combination of the rear feux rouges fluorescent tube for carrying out 5s and ultraviolet lamp tube；
Step 3: after the seedling in greenhouse grows 6~8 leaves, the thick seedling for 1~1.5cm of stem is selected from apart from it
At the 12cm of top cut, expose smooth section, as grafting stock, then from the section 3cm of stock towards section
Oblique partial application, after beveling, the bevel cuts angle at 45 ° of stock；
Step 4: from macadamia it is raw then, it is semi-lignified on the sunny side on branch sheared length be 11~12cm, stem it is thick
Branch for 1~1.5cm, with 1~2 leaf bud is chamfer as fringe bar, and from sheared edge 3cm towards sheared edge, tiltedly
After cutting, the bevel cuts of fringe bar and the angle of its sheared edge are 45 °, and then the fringe bar chamfer is put into nutrient solution and soaked
After 4min, taking-up, which is put into incubator, stores, is standby, wherein, filtrated air is passed through in incubator, controls the temperature in incubator
For 14 DEG C, humidity 88%, nutrient solution includes following raw material：The Nafusaku of 8 parts by weight, the gibberellin of 4 parts by weight, 4 weight
Asparagus juice, the keel extract solution of 5 parts by weight, the root of Dahurain angelica of 7 parts by weight, the ground blood perfume, the Pu'er tea of 15 parts by weight of 2 parts by weight of part
Ferment, the vitamin C of 10 parts by weight, the konjaku flour of 5 parts by weight；
Step 5: spray graft union agent on the bevel cuts of stock and the bevel cuts of fringe bar, and by fringe bar
Bevel cuts are directed at the bevel cuts of stock, are bundled stock and fringe bar with film bandage, and grafting is completed, wherein, the ring of grafting
Border temperature is 26 DEG C, and graft union agent includes the raw material of following parts by weight：2 parts of vitamin A, 3 parts of Eupatorium adenophorum, 0.7 part
Lipoic acid, 4 parts of heteroauxin, 4 parts of polyvinyl alcohol, 2 parts of olive polyphenol, 6 parts of propolis, 0.5 part of potassium citrate,
2.2 parts of chitosan, 0.6 part of garlic selenium polysaccharide, 0.2 part of capric acid.
Wherein, the incubator described in step 2 includes：
Casing 1, it is cube structure, and one of side is can be left positioned at the chamber door 11 with the chamber door 11 of folding
The side inner walls of right both sides along the depth direction of casing 1 respectively between the upper and lower away from being provided with two parallel projections 12, the projection
12 length is less than the depth of the casing 1, and the side where it is divided into three by two described raised 12 of any side
Region, each region along projection 12 bearing of trend between the upper and lower away from set gradually it is multigroup irradiation fluorescent tube 13, the He of chamber door 11
The LED plant lamps 14 that two away from setting power are 45W between the upper and lower respectively on the inwall of side corresponding thereto；
Two culture plates 2, it is cube structure, wherein two relative sides cave inward to be formed and the projection
12 grooves 22 being adapted, so that the culture plate 2 slides along described raised 12, the bottom of culture plate 2 is from edge to center
Multi-turn air-vent 23 is interval with, often encloses the rectangle that is shaped as of air-vent 23, and its cornerwise intersection point and the bottom of culture plate 2
The center superposition in portion, the bottom of the culture plate 2 are made up of transparency silica glass；
Wherein, the LED plant lamps 14 that the top of any one of culture plate 2 is set on the inwall of casing 1 with
Circumference of the irradiation fluorescent tube 13 along the casing 1 forms a circle.
The projection 12 of the incubator is provided with cylindrical locating dowel 121, the locating dowel close to the end face of the chamber door 11
121 are provided with screw thread, the culture plate 2 towards the opposite end of the side of the chamber door 11 be respectively equipped with side one into
The fixed plate of type, the fixed plate are provided with positioning hole 221, wherein, the culture plate 2 slips into the incubator along its groove 22
After interior, the locating dowel 121 is through the positioning hole 221, be spirally connected fixation by nut.
Laying depth ratio is 1 successively for the bottom of culture plate 2:4 flax fiber layer of cloth and growth substrate layer, wherein, it is raw
Long hypothallus is by the fine earth of 7 parts by weight, the vermiculite power of 18 parts by weight, the porous ceramic grain of 15 parts by weight, the sawdust of 12 parts by weight, 9
The macadamia shell powder of parts by weight is mixed.
Step 2 infiltrates flax fiber cloth is screwed onto not draining in the bamboo vinegar aqueous solution that mass fraction is 0.5%, taking-up,
It is layed in the culture tray bottom, then the basic unit of laying growth above it.
Film bandage in step 5 is bandage made of polytetrafluoroethylene film or biological sealed membrane.
<Embodiment 3>
A kind of engrafting method of Queensland nut of the present invention, comprises the following steps：
6h is soaked Step 1: Queensland nut seed is placed in clear water, removes the seed of floating, remaining seed is fished for and is placed in
Vibration immersion 10h in soak, the temperature for controlling soak is 40 DEG C, and kind must be soaked by taking out, wherein, soak is by 22 parts by weight
Tertiary sodium phosphate, the chitosan of 25 parts by weight, the gibberellin of 12 parts by weight, the sodium selenite of 3 parts by weight, 7 parts by weight spirit hair
The deionized water that element is dissolved in 1000 parts by weight is made；
Step 2: immersion kind is placed in into vernalization culture in incubator, cultivation temperature is 25 DEG C, and culture humidity is 80%, light
Be 8h/ days according to temporal summation, exposure time summation be 80min/ days, and illumination with irradiation alternately, treat that seedling grows to 15cm
Afterwards, move into greenhouse and cultivate, wherein, multigroup irradiation fluorescent tube 13 is oppositely arranged in incubator, every group of irradiation fluorescent tube 13 includes one
The ultraviolet lamp tube that the feux rouges fluorescent tube and a wavelength that gold-tinted fluorescent tube that wavelength is 590nm, a wavelength are 810nm are 370nm, and
A feux rouges fluorescent tube and a ultraviolet lamp tube, tool of the illumination with irradiation alternately are arranged side by side directly over one gold-tinted fluorescent tube
Body method is：First carry out 1h illumination, carry out 10min irradiation again；
Wherein, 10min irradiation is that 5 circulations irradiate, and irradiation every time first carries out 115s simple gold-tinted fluorescent tube spoke
Irradiated according to, the combination of the rear feux rouges fluorescent tube for carrying out 5s and ultraviolet lamp tube；
Step 3: after the seedling in greenhouse grows 6~8 leaves, the thick seedling for 1~1.5cm of stem is selected from apart from it
At the 12cm of top cut, expose smooth section, as grafting stock, then from the section 3cm of stock towards section
Oblique partial application, after beveling, the bevel cuts angle at 45 ° of stock；
Step 4: from macadamia it is raw then, it is semi-lignified on the sunny side on branch sheared length be 11~12cm, stem it is thick
Branch for 1~1.5cm, with 1~2 leaf bud is chamfer as fringe bar, and from sheared edge 3cm towards sheared edge, tiltedly
After cutting, the bevel cuts of fringe bar and the angle of its sheared edge are 45 °, and then the fringe bar chamfer is put into nutrient solution and soaked
After 5min, taking-up, which is put into incubator, stores, is standby, wherein, filtrated air is passed through in incubator, controls the temperature in incubator
For 15 DEG C, humidity 90%, nutrient solution includes following raw material：The Nafusaku of 9 parts by weight, the gibberellin of 7 parts by weight, 5 weight
Asparagus juice, the keel extract solution of 7 parts by weight, the root of Dahurain angelica of 8 parts by weight, the ground blood perfume, the Pu'er tea of 17 parts by weight of 2 parts by weight of part
Ferment, the vitamin C of 12 parts by weight, the konjaku flour of 5 parts by weight；
Step 5: spray graft union agent on the bevel cuts of stock and the bevel cuts of fringe bar, and by fringe bar
Bevel cuts are directed at the bevel cuts of stock, are bundled stock and fringe bar with film bandage, and grafting is completed, wherein, the ring of grafting
Border temperature is 28 DEG C, and graft union agent includes the raw material of following parts by weight：3 parts of vitamin A, 3.5 parts of Eupatorium adenophorum, 1 part
Lipoic acid, 6 parts of heteroauxin, 5 parts of polyvinyl alcohol, 2 parts of olive polyphenol, 7 parts of propolis, 0.8 part of potassium citrate,
2.5 parts of chitosan, 0.9 part of garlic selenium polysaccharide, 0.3 part of capric acid.
Wherein, the incubator described in step 2 includes：
Casing 1, it is cube structure, and one of side is can be left positioned at the chamber door 11 with the chamber door 11 of folding
The side inner walls of right both sides along the depth direction of casing 1 respectively between the upper and lower away from being provided with two parallel projections 12, the projection
12 length is less than the depth of the casing 1, and the side where it is divided into three by two described raised 12 of any side
Region, each region along projection 12 bearing of trend between the upper and lower away from set gradually it is multigroup irradiation fluorescent tube 13, the He of chamber door 11
The LED plant lamps 14 that two away from setting power are 45W between the upper and lower respectively on the inwall of side corresponding thereto；
Two culture plates 2, it is cube structure, wherein two relative sides cave inward to be formed and the projection
12 grooves 22 being adapted, so that the culture plate 2 slides along described raised 12, the bottom of culture plate 2 is from edge to center
Multi-turn air-vent 23 is interval with, often encloses the rectangle that is shaped as of air-vent 23, and its cornerwise intersection point and the bottom of culture plate 2
The center superposition in portion, the bottom of the culture plate 2 are made up of transparency silica glass；
Wherein, the LED plant lamps 14 that the top of any one of culture plate 2 is set on the inwall of casing 1 with
Circumference of the irradiation fluorescent tube 13 along the casing 1 forms a circle.
The projection 12 of the incubator is provided with cylindrical locating dowel 121, the locating dowel close to the end face of the chamber door 11
121 are provided with screw thread, the culture plate 2 towards the opposite end of the side of the chamber door 11 be respectively equipped with side one into
The fixed plate of type, the fixed plate are provided with positioning hole 221, wherein, the culture plate 2 slips into the incubator along its groove 22
After interior, the locating dowel 121 is through the positioning hole 221, be spirally connected fixation by nut.
Laying depth ratio is 1 successively for the bottom of culture plate 2:4 flax fiber layer of cloth and growth substrate layer, wherein, it is raw
Long hypothallus is by the fine earth of 8 parts by weight, the vermiculite power of 20 parts by weight, the porous ceramic grain of 16 parts by weight, the sawdust of 14 parts by weight, 10
The macadamia shell powder of parts by weight is mixed.
Step 2 infiltrates flax fiber cloth is screwed onto not draining in the bamboo vinegar aqueous solution that mass fraction is 0.6%, taking-up,
It is layed in the culture tray bottom, then the basic unit of laying growth above it.
Film bandage in step 5 is bandage made of polytetrafluoroethylene film or biological sealed membrane.
<Contrast test>
Comparative example 1 carries out grafting, culture for the stock made in step 2 using the seedling of common incubator cultivation
Temperature is 25 DEG C, and culture humidity is 75%, and light application time is 8h/ days, and without radiation treatment, remaining parameter and step and reality
It is identical to apply example 2；
Comparative example 2 is not soak and be not put into incubator storage, remaining parameter and step in step 4 with nutrient solution to fringe bar
It is rapid same as Example 2.
In areal, while according to embodiment 2, comparative example 1, the method for comparative example 2 respectively to 100 Queensland nut kinds
Son is cultivated, and obtains seedling, is chosen and is made stock, then carries out grafting, obtains 100 plants of graftings, and record is transferred after 45 days
The survival rate for surviving strain number, calculating after grafting of seedlings picking, as shown in table 1.
Survival rate after the grafting of table 1
| The seed germination time | Survival rate after grafting | |
| Embodiment 2 | After 9 days | 97% |
| Comparative example 1 | After 17 days | 90% |
| Comparative example 2 | 15.5 after it | 86% |
After 6 years, embodiment 2, comparative example 1, the Queensland nut of comparative example 2 are harvested respectively, and randomly select 100 Australia respectively
Continent nut, weigh and measure fruit footpath size, calculate the average weight of the single Queensland nut of Queensland nut, average fruit footpath, and
The average oil content data of the benevolence measured after Queensland nut is air-dried respectively are as shown in table 2 below.
The average weight of 2 single Queensland nut of table, average fruit footpath and average oil content
| Mean Fruit Weight/g | Average fruit footpath/cm | Average oil content/% | |
| Embodiment 2 | 9.93 | 2.41 | 78.32 |
| Comparative example 1 | 8.47 | 1.88 | 67.91 |
| Comparative example 2 | 8.53 | 1.96 | 69.84 |
From Tables 1 and 2, using incubator of the invention and according to the Queensland nut after the method grafting of embodiment 2
The seed germination time be significantly faster than that comparative example 1 and comparative example 2, survival rate is up to 97%, the Queensland nut after final grafting
Mean Fruit Weight, average fruit footpath and average oil content are above comparative example 1 and comparative example 2, therefore, after the final grafting of the present invention
Queensland nut fruit quality it is excellent.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art
Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, it is of the invention and unlimited
In specific details and shown here as the legend with description.
Claims (7)
1. a kind of engrafting method of Queensland nut, it is characterised in that comprise the following steps：
Step 1: Queensland nut seed is placed in into 4~6h of immersion in clear water, the seed of floating is removed, remaining seed is fished for and is placed in
In soak vibration immersion 8~10h, control soak temperature be 35~40 DEG C, kind must be soaked by taking out, wherein, soak by
The tertiary sodium phosphate of 17~22 parts by weight, the chitosan of 18~25 parts by weight, the gibberellin of 6~12 parts by weight, 2~3 parts by weight
The deionized water that sodium selenite, the spirit hair element of 4~7 parts by weight are dissolved in 1000 parts by weight is made；
Step 2: immersion kind is placed in into vernalization culture in incubator, cultivation temperature is 25 DEG C, and culture humidity is 70%~80%,
Light application time summation is 8h/ days, exposure time summation is 80min/ days, and illumination treats that seedling grows to 10 with irradiating alternately
After~15cm, move into greenhouse and cultivate, wherein, multigroup irradiation fluorescent tube is oppositely arranged in incubator, every group of irradiation fluorescent tube includes one
The ultraviolet lamp tube that the feux rouges fluorescent tube and a wavelength that gold-tinted fluorescent tube that individual wavelength is 590nm, a wavelength are 810nm are 370nm,
And a feux rouges fluorescent tube and a ultraviolet lamp tube are arranged side by side directly over a gold-tinted fluorescent tube, illumination is with irradiating alternately
Specific method is：First carry out 1h illumination, carry out 10min irradiation again；
Step 3: after the seedling in greenhouse grows 6~8 leaves, the thick seedling for 1~1.5cm of stem is selected from apart from its top
Cut at 12cm, expose smooth section, chamfer as grafting stock, then from the section 3cm of stock towards section
One knife, after beveling, the bevel cuts angle at 45 ° of stock；
Step 4: from macadamia it is raw then, it is semi-lignified on the sunny side on branch sheared length be 11~12cm, stem be slightly 1
~1.5cm, the branch with 1~2 leaf bud are chamfer as fringe bar, and from sheared edge 3cm towards sheared edge, beveling
Afterwards, the bevel cuts of fringe bar and the angle of its sheared edge are 45 °, then fringe bar chamfer is put into nutrient solution soak 3~
After 5min, taking-up, which is put into incubator, stores, is standby, wherein, filtrated air is passed through in incubator, controls the temperature in incubator
For 13~15 DEG C, humidity is 85%~90%, and nutrient solution includes following raw material：The Nafusaku of 6~9 parts by weight, 2~7 weight
Gibberellin, the asparagus juice of 2~5 parts by weight, the keel extract solution of 4~7 parts by weight, the root of Dahurain angelica, 1~2 weight of 5~8 parts by weight of part
Measure ground blood perfume, the Pu'er tea ferment of 13~17 parts by weight, the vitamin C of 7~12 parts by weight, the konjaku of 4~5 parts by weight of part
Powder；
Step 5: spray graft union agent on the bevel cuts of stock and the bevel cuts of fringe bar, and by the inclined-plane of fringe bar
The bevel cuts of cut alignment stock, stock and fringe bar being bundled with film bandage, grafting is completed, wherein, the environment temperature of grafting
Spend for 24~28 DEG C, graft union agent includes the raw material of following parts by weight：2~3 parts of vitamin A, 2.5~3.5 parts of purple stem
Herba Lycopi, 0.5~1 part of lipoic acid, 3~6 parts of heteroauxin, 4~5 parts of polyvinyl alcohol, 1~2 part of olive polyphenol, 5~7
Part propolis, 0.2~0.8 part of potassium citrate, 2~2.5 parts of chitosan, 0.4~0.9 part of garlic selenium polysaccharide, 0.2~
0.3 part of capric acid.
2. the engrafting method of Queensland nut as claimed in claim 1, it is characterised in that wherein, the spoke of the 10min in step 2
According to for 5 circulation irradiation, every time irradiation first carry out 115s simple gold-tinted fluorescent tube irradiation, the rear feux rouges fluorescent tube for carrying out 5s and
The combination irradiation of ultraviolet lamp tube.
3. the engrafting method of Queensland nut as claimed in claim 1, it is characterised in that the culture case and bag described in step 2
Include：
Casing, it is cube structure, and one of side is can be with the chamber door of folding, the side at left and right sides of the chamber door
Respectively between the upper and lower away from two parallel projections are provided with along the cabinet depth direction, the raised length is less than described face inwall
Side where it is divided into three regions by the depth of casing, two projections of any side, and each region is along convex
The bearing of trend risen is distinguished between the upper and lower away from setting gradually multigroup irradiation fluorescent tube on the inwall of the chamber door and side corresponding thereto
The LED plant lamps that two away from setting power are 45W between the upper and lower；
Two culture plates, it is cube structure, is fitted wherein two relative sides cave inward to be formed with the raised phase
The groove matched somebody with somebody, so that the culture plate is interval with multi-turn along the raised slip, the culture tray bottom from edge to center
Air-vent, the rectangle that is shaped as of air-vent, and its cornerwise intersection point and the center superposition of the culture tray bottom are often enclosed, it is described
The bottom of culture plate is made up of transparency silica glass；
Wherein, the LED plant lamps set above any one of culture plate on the cabinet wall and the irradiation
Circumference of the fluorescent tube along the casing forms a circle.
4. the engrafting method of Queensland nut as claimed in claim 3, it is characterised in that the projection of the incubator is close to described
The end face of chamber door is provided with cylindrical locating dowel, and the locating dowel is provided with screw thread, and the culture plate is towards the side of the chamber door
Opposite end be respectively equipped with and be provided with positioning hole with the integrally formed fixed plate in the side, the fixed plate, wherein, the training
After foster disk is slipped into the incubator along its groove, the locating dowel is through the positioning hole, be spirally connected fixation by nut.
5. the engrafting method of Queensland nut as claimed in claim 3, it is characterised in that the culture tray bottom lays thickness successively
Degree is than being 1:4 flax fiber layer of cloth and growth substrate layer, wherein, growth substrate layer by 6~8 parts by weight fine earth, 15~20
The vermiculite power of parts by weight, the porous ceramic grain of 12~16 parts by weight, the sawdust of 11~14 parts by weight, Australia of 8~10 parts by weight are hard
Shell powder is mixed.
6. the engrafting method of Queensland nut as claimed in claim 5, it is characterised in that step 2 infiltrates flax fiber cloth
Mass fraction is 0.4%~0.6% bamboo vinegar aqueous solution, and taking-up is screwed onto not draining, is layed in the culture tray bottom, then
Its top laying growth basic unit.
7. the engrafting method of Queensland nut as claimed in claim 1, it is characterised in that the film bandage in step 5 is poly- four
Bandage made of fluoroethylene film or biological sealed membrane.
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| CN201710899673.9A CN107810730A (en) | 2017-09-28 | 2017-09-28 | The engrafting method of Queensland nut |
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|---|---|---|---|
| CN201710899673.9A CN107810730A (en) | 2017-09-28 | 2017-09-28 | The engrafting method of Queensland nut |
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| CN109006110A (en) * | 2018-07-09 | 2018-12-18 | 广西南亚热带农业科学研究所 | A kind of Queensland nut method for stunting |
| CN109328698A (en) * | 2018-10-25 | 2019-02-15 | 罗城仫佬族自治县金陆地种养农民专业合作社 | The method for culturing seedlings of blood orange |
| CN109874446A (en) * | 2019-02-26 | 2019-06-14 | 贵州省亚热带作物研究所 | A kind of breeding method of Queensland nut seed |
| CN110249824A (en) * | 2019-07-17 | 2019-09-20 | 广西南亚热带农业科学研究所 | A kind of method of injury macadamia reparation |
| CN110393119A (en) * | 2019-07-31 | 2019-11-01 | 广西澳一澳农业科技有限公司 | A kind of Queensland nut seed fast seedling growing propagation method |
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| CN114600695A (en) * | 2022-03-04 | 2022-06-10 | 李琛 | Method for modifying macadimia nut low-yield forest |
| CN114946626A (en) * | 2022-06-28 | 2022-08-30 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Factory light-matrix seedling raising method for promoting growth of macadamia nut and wild longan root |
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| CN110249824A (en) * | 2019-07-17 | 2019-09-20 | 广西南亚热带农业科学研究所 | A kind of method of injury macadamia reparation |
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| CN114600695A (en) * | 2022-03-04 | 2022-06-10 | 李琛 | Method for modifying macadimia nut low-yield forest |
| CN114946626A (en) * | 2022-06-28 | 2022-08-30 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Factory light-matrix seedling raising method for promoting growth of macadamia nut and wild longan root |
| CN114946626B (en) * | 2022-06-28 | 2023-11-17 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Factory light matrix seedling raising method for promoting growth of macadimia nut mountain longan roots |
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