CN103650895B - Cutting breeding method of pseudostellaria heterophylla - Google Patents
Cutting breeding method of pseudostellaria heterophylla Download PDFInfo
- Publication number
- CN103650895B CN103650895B CN201310742279.6A CN201310742279A CN103650895B CN 103650895 B CN103650895 B CN 103650895B CN 201310742279 A CN201310742279 A CN 201310742279A CN 103650895 B CN103650895 B CN 103650895B
- Authority
- CN
- China
- Prior art keywords
- cuttage
- matrix
- cutting
- radix pseudostellariae
- breeding method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a cutting breeding method of pseudostellaria heterophylla. The cutting breeding method of pseudostellaria heterophylla comprises the following steps that in a production greenhouse of virus-free pseudostellaria heterophylla protospecies, strong seedlings with the height being 12-20cm and the ground stem being larger than 1mm and with more than six joints are selected, and a stem section which grows for 4-6cm and has more than four joints is cut to serve as a cutting twig; a seedling bed is prepared; the matrix of the seedling bed is humus soil and perlite, and the ratio between the humus soil and the perlite is 3:1; an cutting operation is carried out, cutting is carried out in the direction perpendicular to the moving direction of a box surface on the seedling bed with the disinfected matrix; a small shed is built after cutting is finished, the humidity is controlled to be about 90%, the temperature is controlled to be 20+/-2 DEG C, the small shed is dismounted after one week, an insect-prevention facility is built, and then common field management on water, fertilizer and the like is carried out. The cutting breeding method of pseudostellaria heterophylla is convenient to operate, high in survival rate, short in period, and suitable for facility planting.
Description
Technical field
The present invention relates to planting technology field, relate in particular to a kind of cuttage breeding method of radix pseudostellariae.
Background technology
Radix pseudostellariae is pinkwort caryophyllaceous ginseng
pseudostellaria heterophylla(Miq.) the block root of Pax ex Pax et Hoffm..When summer, cauline leaf major part was withered, excavates block root and clean, removing fibrous root, put and to dry after slightly scalding in boiling water or directly to dry rear hyoscine.Radix pseudostellariae is gone through edition Pharmacopoeia of the People's Republic of China and is all given and recording.Radix pseudostellariae has and replenishes qi to invigorate the spleen, promoting production of body fluid and nourishing the lung function; Clinical tired for insufficiency of the spleen body, poor appetite, weak after being ill, deficiency of both vital energy and Yin, spontaneous perspiration day is thirsty, the diseases such as dryness of the lung dry cough.Radix pseudostellariae consumption cumulative year after year, wide market.
Mating system major sexual breeding (seminal propagation) and vegetative propagation (block root propagation) of current radix pseudostellariae.
Since the wild change man of radix pseudostellariae plants, Production of Large Fields mainly adopts block root to carry out vegetative propagation, which results in radix pseudostellariae kind and constantly degenerates, and sick worm harm constantly aggravates and then has a strong impact on radix pseudostellariae per unit area yield and quality.In addition, seminal propagation is also one of mode of producing offspring of radix pseudostellariae, because its Capsule maturity is inconsistent, Chang Ziran is scattered, if artificially collect seed by time and effort consuming, still lack the treatment technology to radix pseudostellariae seed Dormancy breaking at present, this causes the germination percentage of radix pseudostellariae seed and germinating energy all lower, then affects output, at present only commodity ginseng gather after or asexual cultivation time, seed with regard to naturally landing adopts the method covering railway carriage or compartment to plant, as auxiliary cultivation step.Therefore, find a kind of make the quality of radix pseudostellariae high, stable yield, can the propagation method of breeding on a large scale be current urgent problem.
Summary of the invention
The object of the invention is to overcome above-mentioned shortcoming and provide a kind of easy to operate, survival rate is high, the cycle is short, is applicable to the radix pseudostellariae cuttage breeding method of facility cultivation.
The cuttage breeding method of a kind of radix pseudostellariae of the present invention, comprises the following steps:
(1) in detoxification radix pseudostellariae seed production booth, the high 12 ~ 20cm of roguing, ground stem is greater than 1 ㎜, the healthy and strong plant that 6 joints are above, cuts into and is about 4 ~ 6cm with the above stem section of 4 joints, be positioned in the container of sterilization with scissors.By shearing lower seedling, being trimmed to inferior end notch and pitch 0.3cm, cutting off the blade that 1 ~ 2, lower end is saved simultaneously, cuttage bar being classified according to clip position, is bundled into wisp, inferior end notch aligns, smooth; The cuttage bar carbendazim (75% wettable powder) of 1000 times of liquid is soaked cuttage bar end 1 minute, is filtered dry and is positioned over clean containers for future use;
(2) preparation in seedbed: medium of seedling bed is fertile soil and perlite, ratio is 3:1, first by matrix scarifying, every square metre with 98%-100% must speed go out fine granule 10-15g add water 3L dilution after spray, then stir matrix 7-10cm, medicament is fully mixed with matrix, covered with plastic film, get final product cuttage after scarifying matrix 2 times after one week;
(3) carry out cuttage operation, the seedbed that matrix has been sterilized is moved towards along face, railway carriage or compartment vertical direction and carries out cuttage, cottage method is:
Dark 3 ~ 4cm aperture is bored with the rod of diameter 0.3 ~ 0.5cm in seedbed, be dip in methyl α-naphthyl acetate (NAA) solution of 1000ppm to put into aperture afterwards in concentration by the cuttage bar handled well, limit insert edge compacting, control spacing in the rows 6 ~ 8cm, line-spacing 8 ~ 10cm, irrigates with 1/10MS minimal medium solution after cuttage;
Or on seedbed, open the shallow ridges of dark 3 ~ 4cm, be dip in the NAA solution of 1000ppm to be placed in shallow ridges by spacing in the rows 6 ~ 8cm afterwards in concentration by the cuttage bar handled well, earthing compacting, then opens shallow ridges by line-spacing 8 ~ 10cm, carry out successively, irrigate with 1/10MS minimal medium solution after cuttage;
(4) build Small plastic shed after cuttage, humidity control is about 90%, and temperature controls, at about 20 ± 2 DEG C, to remove Small plastic shed after one week, sets up insect protected facility, then carries out the field management such as daily water, fertilizer.
In order to verify the exercising result that said method is cultivated radix pseudostellariae cottage propagation, carry out series of experimental research, specific as follows:
Separate sources cuttage bar has been infected by the virus to radix pseudostellariae block root and has contacted directly, this test adopts conventional block root propagation branch, group training detoxic seedling branch, radix pseudostellariae seed seedling branch to be research object, according to identical cutting density, often organizing cuttage 3 ㎡ is a process, repeat for 3 times, by slotting machine matrix sterilization before cuttage, insect protected facility is set up after cuttage, and add up survival rate after 20 days, blade performance is observed in mid-May, late July surveys to produce and detect radix pseudostellariae block root and to be infected by the virus situation, and statistical conditions are as following table:
As can be seen from above data, cuttage survival rate aspect, detoxification radix pseudostellariae is comparatively other two kinds all high, branch comparatively, reaches 98%, and other branches in survival rate about 90%; All part shows floral leaf, mottled floral leaf, shrinkage, distortion distort and the phenomenon of curling trend on having at growth middle leaves for conventional block root propagation branch, radix pseudostellariae seed seedling branch plant, this is the result be infected by the virus, and obtains further confirmation by determination block root Virus Infection; Owing to endangering by virosis, the growth of plant receives and has a strong impact on, and then affects the growth of underground block root, and the output of detoxification radix pseudostellariae plantlet in vitro branch is the highest, reaches 689.78g/ ㎡, and the output of radix pseudostellariae seed seedling branch is minimum, at 498.25g/ ㎡.
The present invention compared with prior art, has obvious beneficial effect, as can be known from the above technical solutions: the present invention is easy to operate, cutting survival, survival rate is high, and breeding coefficient reaches 6 ~ 10 times, and speed is fast, cutting propagation is applicable to radix pseudostellariae facility cultivation, then can output production seed ginseng and medicinal material commodity.The heavy 0.45g of the radix pseudostellariae block root average single using the present invention to produce.Can provide excellent seed ginseng for radix pseudostellariae Production of Large Fields, use the seed ginseng that the present invention breeds, land for growing field crops growth coefficient reaches 10 ~ 15 times.
Detailed description of the invention
embodiment 1:
A cuttage breeding method for radix pseudostellariae, comprises the following steps:
(1) in detoxification radix pseudostellariae seed production booth, the high 12 ~ 20cm of roguing, ground stem is greater than 1 ㎜, the healthy and strong plant that 6 joints are above, cuts into and is about 4 ~ 6cm with the above stem section of 4 joints, be positioned in the container of sterilization with scissors.By shearing lower seedling, being trimmed to inferior end notch and pitch 0.3cm, cutting off the blade that 1 ~ 2, lower end is saved simultaneously, cuttage bar being classified according to clip position, is bundled into wisp, inferior end notch aligns, smooth; The cuttage bar carbendazim (75% wettable powder) of 1000 times of liquid is soaked cuttage bar end 1 minute, is filtered dry and is positioned over clean containers for future use;
(2) preparation in seedbed: medium of seedling bed is fertile soil and perlite, ratio is 3:1, first by matrix scarifying, every square metre with 98%-100% must speed go out fine granule 10-15g add water 3L dilution after spray, then stir matrix 7-10cm, and medicament is fully mixed with matrix, covered with plastic film, got final product cuttage after scarifying matrix 2 times after 1 week;
(3) carry out cuttage operation, the seedbed that matrix has been sterilized is moved towards along face, railway carriage or compartment vertical direction and carries out cuttage, cottage method is:
Dark 3 ~ 4cm aperture is bored with the rod of diameter 0.3 ~ 0.5cm in seedbed, be dip in methyl α-naphthyl acetate (NAA) solution of 1000ppm to put into aperture afterwards in concentration by the cuttage bar handled well, limit insert edge compacting, control spacing in the rows 6 ~ 8cm, line-spacing 8 ~ 10cm, irrigates with 1/10MS minimal medium solution after cuttage;
(4) build Small plastic shed after cuttage, humidity control is about 90%, and temperature controls, at about 20 ± 2 DEG C, to remove Small plastic shed after one week, sets up insect protected facility, then carries out the field management such as daily water, fertilizer.
embodiment 2:
A cuttage breeding method for radix pseudostellariae, comprises the following steps:
(1) in detoxification radix pseudostellariae seed production booth, the high 12 ~ 20cm of roguing, ground stem is greater than 1 ㎜, the healthy and strong plant that 6 joints are above, cuts into and is about 4 ~ 6cm with the above stem section of 4 joints, be positioned in the container of sterilization with scissors.By shearing lower seedling, being trimmed to inferior end notch and pitch 0.3cm, cutting off the blade that 1 ~ 2, lower end is saved simultaneously, cuttage bar being classified according to clip position, is bundled into wisp, inferior end notch aligns, smooth; The cuttage bar carbendazim (75% wettable powder) of 1000 times of liquid is soaked cuttage bar end 1 minute, is filtered dry and is positioned over clean containers for future use;
(2) preparation in seedbed: medium of seedling bed is fertile soil and perlite, ratio is 3:1, first by matrix scarifying, every square metre with 98%-100% must speed go out fine granule 10-15g add water 3L dilution after spray, then stir matrix 7-10cm, and medicament is fully mixed with matrix, covered with plastic film, got final product cuttage after scarifying matrix 2 times after 1 week;
(3) cuttage operation is carried out, the seedbed that matrix has been sterilized is moved towards along face, railway carriage or compartment vertical direction and carries out cuttage, cottage method is: the shallow ridges opening dark 3 ~ 4cm on seedbed, be dip in methyl α-naphthyl acetate (NAA) solution of 1000ppm to be placed in shallow ridges by spacing in the rows 6 ~ 8cm afterwards in concentration by the cuttage bar handled well, earthing compacting, then open shallow ridges by line-spacing 8 ~ 10cm, carry out successively, irrigate with 1/10MS minimal medium solution after cuttage;
(4) build Small plastic shed after cuttage, humidity control is about 90%, and temperature controls, at about 20 ± 2 DEG C, to remove Small plastic shed after one week, sets up insect protected facility, then carries out the field management such as daily water, fertilizer.
Claims (2)
1. a cuttage breeding method for radix pseudostellariae, comprises the following steps:
(1) in detoxification radix pseudostellariae seed production booth, high 12 ~ the 20cm of roguing, ground stem is greater than 1 ㎜, the healthy and strong plant that 6 joints are above, cut growth 4 ~ 6cm and save above stem section with 4, be positioned in the container of sterilization, by the seedling under shearing, be trimmed to inferior end notch and pitch 0.3cm, cut off the blade of 1 ~ 2, lower end joint simultaneously, cuttage bar is classified according to clip position, is bundled into wisp, inferior end notch alignment, smooth; Cuttage bar carbendazim 75% wettable powder of 1000 times of liquid is soaked cuttage bar end 1 minute, is filtered dry and is positioned over clean containers for future use;
(2) preparation in seedbed: medium of seedling bed is fertile soil and perlite, ratio is 3:1, first by matrix scarifying, every square metre with 98%-100% must speed go out fine granule 10-15g add water 3L dilution after spray, then stir matrix 7-10cm, medicament is fully mixed with matrix, covered with plastic film, get final product cuttage after scarifying matrix 2 times after 1 week;
(3) cuttage operation is carried out, the seedbed that matrix has been sterilized is moved towards along face, railway carriage or compartment vertical direction and carries out cuttage, cottage method is: on seedbed, bore dark 3 ~ 4cm aperture with the rod of diameter 0.3 ~ 0.5cm, be dip in the naphthalene acid solution of 1000ppm to put into aperture afterwards in concentration by the cuttage bar handled well, limit insert edge compacting, control spacing in the rows 6 ~ 8cm, line-spacing 8 ~ 10cm, irrigates with 1/10MS minimal medium solution after cuttage;
(4) build Small plastic shed after cuttage, humidity control is about 90%, and temperature controls, at 20 ± 2 DEG C, to remove Small plastic shed after one week, sets up insect protected facility, then carries out daily water, fertile field management.
2. the cuttage breeding method of a kind of radix pseudostellariae as claimed in claim 1, wherein cottage method is: the shallow ridges opening dark 3 ~ 4cm on seedbed, be dip in the naphthalene acid solution of 1000ppm to be placed in shallow ridges by spacing in the rows 6 ~ 8cm afterwards in concentration by the cuttage bar handled well, earthing compacting, then shallow ridges is opened by line-spacing 8 ~ 10cm, carry out successively, irrigate with 1/10MS minimal medium solution after cuttage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310742279.6A CN103650895B (en) | 2013-12-30 | 2013-12-30 | Cutting breeding method of pseudostellaria heterophylla |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310742279.6A CN103650895B (en) | 2013-12-30 | 2013-12-30 | Cutting breeding method of pseudostellaria heterophylla |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103650895A CN103650895A (en) | 2014-03-26 |
| CN103650895B true CN103650895B (en) | 2015-03-25 |
Family
ID=50290615
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310742279.6A Expired - Fee Related CN103650895B (en) | 2013-12-30 | 2013-12-30 | Cutting breeding method of pseudostellaria heterophylla |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103650895B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104380965A (en) * | 2014-11-03 | 2015-03-04 | 贵州省植物园 | Production method for radix pseudostellariae roots |
| CN106258295A (en) * | 2015-06-24 | 2017-01-04 | 黄保成 | A kind of method for culturing seedlings of Brachystemma calycina D. Don. |
| CN105123199A (en) * | 2015-08-23 | 2015-12-09 | 林平 | Cutting seedling growing method for stems below top shoots of stevia rebaudiana |
| CN108377772A (en) * | 2018-02-09 | 2018-08-10 | 贵州兴康农业科技有限公司 | A kind of cuttage breeding method of Chinese mesona herb |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101536644A (en) * | 2009-04-20 | 2009-09-23 | 北京金桐福绿色能源科技有限公司 | Method for fast asexual propagation by cottage at root and sowing roots of shinyleaf yellowhorn |
| CN101707959A (en) * | 2009-11-30 | 2010-05-19 | 宁波市农业科学研究院 | Method for raising seedlings of llex integra by cutting |
| CN102668833A (en) * | 2012-05-03 | 2012-09-19 | 贵州玉清生物科技开发有限公司 | Method for planting radix pseudostellariae |
| CN102907238A (en) * | 2012-11-12 | 2013-02-06 | 吉首大学 | Cuttage seedling-culture technology for Chinese trichosanthes |
| CN103109672A (en) * | 2013-03-12 | 2013-05-22 | 刘春生 | Planting method of radix pseudostellariae |
-
2013
- 2013-12-30 CN CN201310742279.6A patent/CN103650895B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101536644A (en) * | 2009-04-20 | 2009-09-23 | 北京金桐福绿色能源科技有限公司 | Method for fast asexual propagation by cottage at root and sowing roots of shinyleaf yellowhorn |
| CN101707959A (en) * | 2009-11-30 | 2010-05-19 | 宁波市农业科学研究院 | Method for raising seedlings of llex integra by cutting |
| CN102668833A (en) * | 2012-05-03 | 2012-09-19 | 贵州玉清生物科技开发有限公司 | Method for planting radix pseudostellariae |
| CN102907238A (en) * | 2012-11-12 | 2013-02-06 | 吉首大学 | Cuttage seedling-culture technology for Chinese trichosanthes |
| CN103109672A (en) * | 2013-03-12 | 2013-05-22 | 刘春生 | Planting method of radix pseudostellariae |
Non-Patent Citations (3)
| Title |
|---|
| 太子参栽培技术;叶传财;《安徽农学通报》;20111231;第17卷(第18期);109-113 * |
| 宣州太子参规范化生产操作规程研究;姚勇等;《现代中药研究与实践》;20041231;第18卷(第5期);28-30 * |
| 黄渡乡太子参高效栽培技术初探;孙厚友等;《安徽农业》;20041231(第6期);38-39 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103650895A (en) | 2014-03-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102986445B (en) | Process for cutting thick branches of golden camellia | |
| CN102227976B (en) | Litsea cubeba pers asexual quick propagation and seedling breeding method | |
| CN105557425B (en) | A kind of method of Chinese chestnut skeleton branch baldness with multiple spot grafting bearing basal shoot | |
| CN102763551B (en) | Cuttage and breeding technology for ampelopsis grossedentata | |
| JP2011142903A (en) | Method for raising seedlings of peony | |
| CN101699956B (en) | Method for tender branch cutting propagation of Linze jujube | |
| CN101563996A (en) | Jiuqian plum tree engrafting and cultivating method | |
| CN1922987A (en) | Multiple-ear rock lithocarpus glaber dwarfing rapid reproduction method | |
| CN104041273A (en) | Potting domestication method for traditional Chinese medicine stevia rebaudiana | |
| CN107455259B (en) | Cultivation method of beautiful millettia root | |
| CN103329770A (en) | New regulation of blueberry planting technology in subtropical regions | |
| CN103798049A (en) | Lonicera japonica twig cutting cultivation method | |
| CN103650895B (en) | Cutting breeding method of pseudostellaria heterophylla | |
| CN105746282B (en) | Rubber tree budling nursery proliferation and rejuvenation thereafter and method for changing | |
| CN104429480A (en) | Taxodium zhongshansha 406 hard wood cutting simple culture method | |
| CN109496656A (en) | A method of improving Folium Ginkgo quality and harvest efficiency | |
| CN105918121A (en) | Method for rapidly breeding seedlings by using Giantleaf Ardisia leaves | |
| CN106472061B (en) | Licorice root cutting breeding method | |
| CN107027627A (en) | A kind of micro-tuber propagation method of David's-harp IMMATURE EMBRYOS CULTURE | |
| CN105993790A (en) | White tea hardwood branch cutting seedling method | |
| CN105009913A (en) | Honeysuckle potting technology | |
| CN101731090A (en) | Cutting propagation method of seedless watermelons | |
| CN104488679A (en) | Acerpalmatum thunbf cuttage sapling cultivation method and formula | |
| CN108719067A (en) | A kind of tissue culture and rapid propagation method of paris polyphylla | |
| CN107517715A (en) | A kind of implantation methods of the stem of noble dendrobium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160810 Address after: 557500, Guizhou Town, Danzhai County, Xingren province camp village Patentee after: Chang Huang Huang (Guizhou) Chinese Medicine Co., Ltd. Address before: 556013 box 204, Qiandongnan Miao and Dong Autonomous Prefecture, Guizhou, Kaili Patentee before: Guizhou Changhao Traditional Chinses Medicine Development Co.,Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150325 Termination date: 20161230 |