CN105418268A - Culture medium and culturing method for flammulina velutipes - Google Patents
Culture medium and culturing method for flammulina velutipes Download PDFInfo
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- CN105418268A CN105418268A CN201510990664.1A CN201510990664A CN105418268A CN 105418268 A CN105418268 A CN 105418268A CN 201510990664 A CN201510990664 A CN 201510990664A CN 105418268 A CN105418268 A CN 105418268A
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- manioc waste
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
- C05F1/005—Fertilisers made from animal corpses, or parts thereof from meat-wastes or from other wastes of animal origin, e.g. skins, hair, hoofs, feathers, blood
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention relates to a culture medium and a culturing method for flammulina velutipes and belongs to the field of edible mushroom cultivation. The culture medium is prepared from cassava residue, conch shells, chestnut shells, beer residue, soybean powder, wood chip, folium artemisiae argyi and indolebutyric acid. In the culture medium, carbon and nitrogen sources, mineral elements, auxins and the like are combined reasonably, so that the culture medium is suitable for growth of the flammulina velutipes. The added folium artemisiae argyi can inhibit growth of bacteria. The flammulina velutipes has a refresh and tender mouth feel and is delicions. The culturing method can increase the quality and yield of the flammulina velutipes.
Description
Technical field
The present invention relates to a kind of culture medium for golden mushroom and cultivating method, belong to field of edible fungus culture.
Background technology
Water content 89.73g, protein 2.72g, fatty 0.13g, ash content 0.83g, sugared 5.45g, robust fibre 0.87g, iron 0.22mg, calcium 0.097mg, phosphorus 1.48mg, sodium 0.22mg, magnesium 0.31mg, vitaminB10 .29mg, Catergen .27mg in the fresh needle mushroom of every hectogram; Needle mushroom is containing 18 seed amino acids, the dry needle mushroom of every 100g is containing amino acid 20.9g, wherein human body must 8 seed amino acids be 44.5% of total amount, higher than general mushroom class, Methionin (lys) in needle mushroom and arginine (Arg) content higher, effectively can promote growing up healthy and sound and intelligent growth of children, thus be called " increasing intelligence mushroom ".From acupuncture needle deep drainpipe mycelium, extract a kind of protein of POF " former Flammulin " that cries not sugary, and and needle mushroom sporophore in " Flammulin " that extract have and have restraining effect to sarcoma S-180 etc.
For edible, the medicinal needle mushroom having very large demand, in order to satisfy social needs, start to plant needle mushroom in enormous quantities, those skilled in the art also on affecting needle mushroom nutrition, the factor of quality and output is studied, and finds that the impact on needle mushroom nutrition, quality and output of different plantation formula materials and cultivating method is very large.
Therefore, for those skilled in the art, need badly develop the most applicable a kind of needle mushroom substratum and cultivating method to plant needle mushroom.
Summary of the invention
The present invention seeks to overcome the deficiencies in the prior art, having invented a kind of new substratum and cultivating method, the yield of flammulina velutipes produced is large, and mouthfeel is good.
The present invention adopts following technical scheme: a kind of culture medium for golden mushroom, is made up of following parts by weight raw material: manioc waste 17 ~ 20 parts, 13 ~ 15 parts, spiral shell shell, 11 ~ 16 parts, chestnut shell, beer residue 10 ~ 13 parts, analysis for soybean powder 8 ~ 10 parts, wood chip 6 ~ 8 parts, tarragon 3 ~ 5 parts, indolebutyric acid 1 ~ 2 part.
Preferably: manioc waste 18 parts, 14 parts, spiral shell shell, 12 parts, chestnut shell, beer residue 12 parts, analysis for soybean powder 9 parts, wood chip 7 parts, tarragon 4 parts, indolebutyric acid 1.5 parts.
Preferably: described manioc waste is the by product after cassava extracts starch, and in massfraction, described manioc waste starch content is 40 ~ 45%.
A kind of above-mentioned substratum cultivating flammulina velutipes method of use, comprises the following steps:
1) get formula ratio raw material, spiral shell shell, chestnut shell are pulverized, add manioc waste, beer residue, analysis for soybean powder, wood chip, tarragon, indolebutyric acid, mixing, adds water, makes moisture content in medium account for 60 ~ 65% of substratum gross weight;
2) substratum is packed sterilizing, to the culture medium inoculated Needle mushroom strain after sterilizing, carry out sending out bacterium;
3) mycelium culture: after mycelium germination field planting, cultivates 20 ~ 30 days in 25 ~ 27 DEG C;
4) carry out conventional management of producing mushroom, gather in time.
Sterilising temp preferably: step 2) is 110 ~ 120 DEG C, and sterilization time is 30 ~ 40 minutes.
The atmospheric moisture of mycelium culture preferably: step 3) is 50 ~ 55%.
In the process of mycelium culture preferably: step 3), cultivating chamber ventilates once every day, and each ventilation time is 30 ~ 40 minutes.
In the process of management of producing mushroom preferably: step 4), atmospheric moisture is 80 ~ 90%.
The collocation such as carbon nitrogen source, mineral element, growth hormone contained by substratum of the present invention rationally, is suitable for the growth of needle mushroom, the growth of the composition energy anti-bacteria contained by the tarragon of adding, and the needle mushroom taste cultivating gained is fresh and tender, delicious good to eat.By cultivating method of the present invention, the quality of needle mushroom and output are all increased.
Embodiment
Further describe technical scheme of the present invention below, but described in claimed scope is not limited to.Below in conjunction with specific embodiment, elaboration detailed is further done to the present invention, but embodiments of the present invention are not limited to the scope that embodiment represents.These embodiments only for illustration of the present invention, but not for limiting the scope of the invention.In addition, after reading content of the present invention, those skilled in the art can do various amendment to the present invention, and these equivalent variations fall within appended claims limited range of the present invention equally.
Embodiment 1
Get the raw material of following weight (kilogram):
Manioc waste 17 spiral shell shell 13 chestnut shell 11 beer residue 10 analysis for soybean powder 8 wood chip 6 tarragon 3 indolebutyric acid 1
Described manioc waste is the by product after cassava extracts starch, and in massfraction, described manioc waste starch content is 40%, and described beer residue is Brewage gained by product.Spiral shell shell, chestnut shell are pulverized, add manioc waste, beer residue, analysis for soybean powder, wood chip, tarragon, indolebutyric acid, mixing, adds water, makes moisture content in medium account for 60% of substratum gross weight.Get the polypropylene plastics bacterium bag that length and width is 30cm × 10cm, substratum loads in bacterium bag, and loading amount is 60% of bag volume, every sacked material 1kg, and bag one end tearing film is tightened, and the other end sack puts plastic hoop, is stoppered tampon.Then sterilizing 30 minutes under 110 DEG C of temperature condition, treat that temperature drops to less than 30 DEG C inoculations, one bottle of bacterial classification can inoculate 50 bags, and inoculation is aseptically carried out.Mycelium culture: after mycelium germination field planting, in 25 DEG C, cultivate 20 days under 50% air humidity conditions, mycelia can cover with full bacterium bag, and in mycelium culture process, cultivating chamber ventilates once every day, and each ventilation time is 30 minutes.Be displaced in cultivating chamber by the bacterium bag covering with mycelia and urge mushroom, described cultivating chamber keeps room temperature 12 DEG C, relative air humidity 80%.After 10 days, pull out tampon, give the scattered light of 50lux, cultivating chamber ventilates 1 time every day, each 20 minutes, after the old mycelia on surface, sack place is wiped off, sack newspaper is built, water spray, after little sporophore occurs, room temperature is down to 4 DEG C, after 10 days, then returns to room temperature 15 DEG C, gather in time.
Embodiment 2
Get the raw material of following weight (kilogram):
Manioc waste 18 spiral shell shell 14 chestnut shell 12 beer residue 12 analysis for soybean powder 9 wood chip 7 tarragon 4 indolebutyric acid 1.5
Described manioc waste is the by product after cassava extracts starch, and in massfraction, described manioc waste starch content is 42%, and described beer residue is Brewage gained by product.Spiral shell shell, chestnut shell are pulverized, add manioc waste, beer residue, analysis for soybean powder, wood chip, tarragon, indolebutyric acid, mixing, adds water, makes moisture content in medium account for 63% of substratum gross weight.Get the polypropylene plastics bacterium bag that length and width is 30cm × 10cm, substratum loads in bacterium bag, and loading amount is 60% of bag volume, every sacked material 1kg, and bag one end tearing film is tightened, and the other end sack puts plastic hoop, is stoppered tampon.Then sterilizing 35 minutes under 115 DEG C of temperature condition, treat that temperature drops to less than 30 DEG C inoculations, one bottle of bacterial classification can inoculate 50 bags, and inoculation is aseptically carried out.Mycelium culture: after mycelium germination field planting, in 26 DEG C, cultivate 25 days under 52% air humidity conditions, mycelia can cover with full bacterium bag, and in mycelium culture process, cultivating chamber ventilates once every day, and each ventilation time is 35 minutes.Be displaced in cultivating chamber by the bacterium bag covering with mycelia and urge mushroom, described cultivating chamber keeps room temperature 12 DEG C, relative air humidity 85%.After 10 days, pull out tampon, give the scattered light of 50lux, cultivating chamber ventilates 1 time every day, each 20 minutes, after the old mycelia on surface, sack place is wiped off, sack newspaper is built, water spray, after little sporophore occurs, room temperature is down to 4 DEG C, after 10 days, then returns to room temperature 15 DEG C, gather in time.
Embodiment 3
Get the raw material of following weight (kilogram):
Manioc waste 20 spiral shell shell 15 chestnut shell 16 beer residue 13 analysis for soybean powder 10 wood chip 8 tarragon 5 indolebutyric acid 2
Described manioc waste is the by product after cassava extracts starch, and in massfraction, described manioc waste starch content is 45%, and described beer residue is Brewage gained by product.Spiral shell shell, chestnut shell are pulverized, add manioc waste, beer residue, analysis for soybean powder, wood chip, tarragon, indolebutyric acid, mixing, adds water, makes moisture content in medium account for 65% of substratum gross weight.Get the polypropylene plastics bacterium bag that length and width is 30cm × 10cm, substratum loads in bacterium bag, and loading amount is 60% of bag volume, every sacked material 1kg, and bag one end tearing film is tightened, and the other end sack puts plastic hoop, is stoppered tampon.Then sterilizing 30 ~ 40 minutes under 120 DEG C of temperature condition, treat that temperature drops to less than 30 DEG C inoculations, one bottle of bacterial classification can inoculate 50 bags, and inoculation is aseptically carried out.Mycelium culture: after mycelium germination field planting, in 27 DEG C, cultivate 30 days under 55% air humidity conditions, mycelia can cover with full bacterium bag, and in mycelium culture process, cultivating chamber ventilates once every day, and each ventilation time is 40 minutes.Be displaced in cultivating chamber by the bacterium bag covering with mycelia and urge mushroom, described cultivating chamber keeps room temperature 12 DEG C, relative air humidity 90%.After 10 days, pull out tampon, give the scattered light of 50lux, cultivating chamber ventilates 1 time every day, each 20 minutes, after the old mycelia on surface, sack place is wiped off, sack newspaper is built, water spray, after little sporophore occurs, room temperature is down to 4 DEG C, after 10 days, then returns to room temperature 15 DEG C, gather in time.
Claims (7)
1. a culture medium for golden mushroom, it is characterized in that, described substratum is made up of following parts by weight raw material: manioc waste 17 ~ 20 parts, 13 ~ 15 parts, spiral shell shell, 11 ~ 16 parts, chestnut shell, beer residue 10 ~ 13 parts, analysis for soybean powder 8 ~ 10 parts, wood chip 6 ~ 8 parts, tarragon 3 ~ 5 parts, indolebutyric acid 1 ~ 2 part.
2. substratum according to claim 1, it is characterized in that, described substratum is made up of following parts by weight raw material: manioc waste 18 parts, 14 parts, spiral shell shell, 12 parts, chestnut shell, beer residue 12 parts, analysis for soybean powder 9 parts, wood chip 7 parts, tarragon 4 parts, indolebutyric acid 1.5 parts.
3. substratum according to claim 1, is characterized in that, described manioc waste is the by product after cassava extracts starch, and in massfraction, described manioc waste starch content is 40 ~ 45%.
4. use the arbitrary described substratum cultivating flammulina velutipes method of claim 1-3, comprise the following steps:
1) get formula ratio raw material, spiral shell shell, chestnut shell are pulverized, add manioc waste, beer residue, analysis for soybean powder, wood chip, tarragon, indolebutyric acid, mixing, adds water, makes moisture content in medium account for 60 ~ 65% of substratum gross weight;
2) substratum is packed sterilizing, to the culture medium inoculated Needle mushroom strain after sterilizing, carry out sending out bacterium;
3) mycelium culture: after mycelium germination field planting, cultivates 20 ~ 30 days in 25 ~ 27 DEG C;
4) carry out conventional management of producing mushroom, gather in time.
Sterilising temp preferably: step 2) is 110 ~ 120 DEG C, and sterilization time is 30 ~ 40 minutes.
5. cultivating method according to claim 4, is characterized in that, step 3) described in mycelium culture atmospheric moisture be 50 ~ 55%.
6. cultivating method according to claim 4, is characterized in that, step 3) described in mycelium culture process, cultivating chamber ventilates once every day, and each ventilation time is 30 ~ 40 minutes.
7. cultivating method according to claim 4, is characterized in that, step 4) in, in described management of producing mushroom process, atmospheric moisture is 80 ~ 90%.
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Citations (6)
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---|---|---|---|---|
CN101508599A (en) * | 2009-03-19 | 2009-08-19 | 詹文园 | Method for producing high-quality edible mushroom culture medium with manioc waste and vinegar slag as raw material |
CN102476974A (en) * | 2010-11-23 | 2012-05-30 | 天津三农金科技有限公司 | Water retaining agent for edible fungi |
CN103319276A (en) * | 2013-07-16 | 2013-09-25 | 王龙 | Golden mushroom medium and its preparation method |
CN103508806A (en) * | 2013-09-27 | 2014-01-15 | 合肥市天丰菌业科技有限公司 | Selenium-rich needle mushroom cultivation material and preparation method thereof |
CN104041328A (en) * | 2014-07-03 | 2014-09-17 | 抚顺清道食用菌科技有限公司 | Golden needle mushroom cultivation method |
CN104119138A (en) * | 2014-06-26 | 2014-10-29 | 安徽中祝农业发展有限公司 | Flammulina velutipes culture medium by using chestnut shells as raw material and preparation method of culture medium |
-
2015
- 2015-12-25 CN CN201510990664.1A patent/CN105418268A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101508599A (en) * | 2009-03-19 | 2009-08-19 | 詹文园 | Method for producing high-quality edible mushroom culture medium with manioc waste and vinegar slag as raw material |
CN102476974A (en) * | 2010-11-23 | 2012-05-30 | 天津三农金科技有限公司 | Water retaining agent for edible fungi |
CN103319276A (en) * | 2013-07-16 | 2013-09-25 | 王龙 | Golden mushroom medium and its preparation method |
CN103508806A (en) * | 2013-09-27 | 2014-01-15 | 合肥市天丰菌业科技有限公司 | Selenium-rich needle mushroom cultivation material and preparation method thereof |
CN104119138A (en) * | 2014-06-26 | 2014-10-29 | 安徽中祝农业发展有限公司 | Flammulina velutipes culture medium by using chestnut shells as raw material and preparation method of culture medium |
CN104041328A (en) * | 2014-07-03 | 2014-09-17 | 抚顺清道食用菌科技有限公司 | Golden needle mushroom cultivation method |
Non-Patent Citations (1)
Title |
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刘春如: "金针菇高产培养基的研究", 《中国食用菌》 * |
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Application publication date: 20160323 |