CN103319276A - Golden mushroom medium and its preparation method - Google Patents
Golden mushroom medium and its preparation method Download PDFInfo
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- CN103319276A CN103319276A CN2013102975764A CN201310297576A CN103319276A CN 103319276 A CN103319276 A CN 103319276A CN 2013102975764 A CN2013102975764 A CN 2013102975764A CN 201310297576 A CN201310297576 A CN 201310297576A CN 103319276 A CN103319276 A CN 103319276A
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Abstract
The invention discloses a golden mushroom medium and its preparation method. The formula of the medium comprises: by weight, 40-50% of herb of alligator alternanthera, 20-25% of rice straw, 3-5% of soybean meal, 10-15% of soluble starch, 1-3% of gypsum, 0.5-1% of calcium superphosphate and 0.5-1% of magnesium sulfate. According to the golden mushroom culture method, its manufacturing and production costs are low, production costs are saved, and economic benefit of golden mushroom is raised. Meanwhile, the grown golden mushroom contains other nutrient contents which are not contained in other golden mushrooms. Thus, nutritive value of golden mushroom is raised.
Description
Technical field
The present invention relates to the making field of biological medium, relate in particular to a kind of culture medium for golden mushroom and preparation method thereof.
Background technology
Genus flammulina is in Agaricales Tricholomataceae money Pseudomonas, with its cap sliding tender, handle is crisp, nutritious, delicious agreeable to the taste and be world-famous for, the first-class food materials of cold vegetable dish in sauce and chafing dish particularly, it is nutritious, A sweety scent assails the nostrils and delicious flavour, is deeply loved by the public.According to surveying and determination, the amino acid whose content of needle mushroom is very abundant, and the content that is higher than general mushroom class, especially Methionin is high especially, and Methionin has the function that promotes children ' s intelligence development.Contain protein 8.87% in the needle mushroom dry product, carbohydrate 60.2%, robust fibre reaches 7.4%, often eats and can prevent and treat Peptic Ulcers.Have research to show again, contained a kind of material has good antitumous effect in the needle mushroom.
Alternanthera philoxeroides originates in Brazil, has another name called revolution grass, water peanut, Alternanthera philoxeroides.Nineteen thirty is imported China into, is the great invasive species of hazardness.Grow in the area of 50 meters ~ 2,700 meters of height above sea level; Grow in pond and the ditch more.Pharmaceutical use is arranged, be listed in the first batch of Invasive Alien Species of China.Contain flavonoid glycoside, triterpenoid saponin, organic acid, phenolic constituent, tonka bean camphor, sugar, fat, protein, 6-chrysoeriol, 7-a-L-rhamnoside and patuletin in the chemical composition of alternanthera philoxeroides.
The needle mushroom artificial cultivation technique is also uncomplicated, as long as can control envrionment conditions well, just easily obtain reliable and stable output, need nitrogenous source, carbon source, inorganic salt etc. to cultivate key element in its substratum, what the nitrogenous source of daily cultivation needle mushroom was selected is corn cob or cotton seed hulls, and the use cost of cultivating by agricultural-food is larger, and the nutritive value of the needle mushroom that grows out and composition are less, composition is single, is unfavorable for the eutrophic absorption of people.And the amino acid of contained necessity in the Growth of Flammulina Velutipes that contains in the alternanthera philoxeroides of dry product can provide the growth nitrogenous source of needle mushroom necessity.
Summary of the invention
For the problem of above-mentioned existence, culture medium for golden mushroom that provides a kind of convenience and high-efficiency and preparation method thereof is provided the object of the invention.
In order to achieve the above object, the technical solution used in the present invention is as follows: a kind of culture medium for golden mushroom, prescription and the weight ratio of described substratum are as follows: the alternanthera philoxeroides of 40%-50%, the rice straw of 20%-25%, analysis for soybean powder 3%-5%, Zulkovsky starch 10%-15%, gypsum 1%-3%, calcium superphosphate 0.5%-1% and sal epsom 0.5%-1%.
The weight of described alternanthera philoxeroides of the present invention is the alternanthera philoxeroides weight after drying, and moist alternanthera philoxeroides not only is inconvenient to weigh, and easily regrows, and is unfavorable for the growth of needle mushroom.
The weight ratio of described alternanthera philoxeroides of the present invention and rice straw is 2:1, alternanthera philoxeroides provides Growth of Flammulina Velutipes necessary nitrogenous source, stalk provides Growth of Flammulina Velutipes required carbon source, also contain carbon, the ample supply of nitrogen element when the alternanthera philoxeroides that content is larger can guarantee Growth of Flammulina Velutipes in the alternanthera philoxeroides simultaneously.
Contain a small amount of moisture in the described substratum of the present invention, described water is distilled water, the weight ratio of moisture and substratum weight ratio are 1:1.5, need moisture in the growing environment of needle mushroom, very few moisture is unfavorable for the needle mushroom normal growth, too much moisture easily causes medium liquid, is unfavorable for the stable typing of substratum and the located growth of Strains of Flammulina velutipes.
After high-temperature boiling crushing, stirring 1h, the filtrate of gained after double gauze filters gets analysis for soybean powder to analysis for soybean powder of the present invention after filtrate dries under aseptic condition by common soya bean; Analysis for soybean powder provides necessary amino acid for Growth of Flammulina Velutipes, but in use needs to remove bacterium wherein, prevents the somatomedin of other mushrooms plunder needle mushroom, guarantees the normal growth of needle mushroom.
Zulkovsky starch of the present invention is by one or more mixtures in sweet potato starch, Starch rice and the potato starch, generally the starch with rice, corn, millet, potato all can be made into Zulkovsky starch, but the Zulkovsky starch that makes with sweet potato starch is best in quality; The particle of sweet potato starch is thicker, and the glued membrane that wrap the outside easily breaks when being hydrolyzed, and is easily dry.
Gypsum of the present invention is that plaster stone is through calcining and the levigate white lime powder that forms and the mixture of plaster stone, blending ratio is 5:1, by add a small amount of unslaked lime in white lime, mixing with distilled water is easily to produce a small amount of heat, improves mixing efficiency and mixed effect.
Culture medium for golden mushroom of the present invention the preferred weight percent proportioning of each composition be: alternanthera philoxeroides 45%, rice straw 22.5%, analysis for soybean powder 3%, Zulkovsky starch 11%, gypsum 1.5%, calcium superphosphate 0.6%, sal epsom 0.8%, the quality per distribution ratio of the distilled water that wherein mixes is 56.2%.
The collocation method of a kind of culture medium for golden mushroom of the present invention:
1) the alternanthera philoxeroides live body is removed root and processed, through sunlight exposure, remove the large quantity of moisture in the alternanthera philoxeroides live body; Alternanthera philoxeroides could normally be used after need to drying inactivation, not so not only can affect the content of nitrogen element in the substratum, contains simultaneously activated alternanthera philoxeroides in the situation that there is the moisture existence easily to regrow;
2) will dry rear live body water content be 10%-15% alternanthera philoxeroides stir to pulverize 1h through pulverizer, described alternanthera philoxeroides the weight of interior moisture content count the substratum weight ratio; The alternanthera philoxeroides particle that obtains 0.8-1CM by the filter screen screening is finished in stirring, and particle is weighed rear stand-by; The alternanthera philoxeroides particle of this diameter makes things convenient for the adhering to of root system of needle mushroom;
3) simultaneously the rice straw of water content 5%-10% is also pulverized through the pulverizer stirring, described rice straw is the rice straw commonly used rice straw that oven dry makes under hot conditions behind washing immersion 3h; The stalk particle that obtains 0.5-0.6CM by the filter screen screening is finished in stirring; Tiny stalk particle makes things convenient for the needle mushroom root system to the absorption of its nutrition, improves growth efficiency;
4) pass through coagulation stirrer mixed weight than being the alternanthera philoxeroides particle of 40%-50% and 20%-25% rice straw particle, it is divided into many groups mixes, one group the time that mixes is 0.5-1h; Improve mixing efficiency, every group is mixed the weight of alternanthera philoxeroides and the weight ratio of rice straw is 2:1, will organize mixed composite grain through the high-pressure steam sterilizing pan 18h that sterilizes more, and the sterilising temp sterilising temp is 100-120 degree centigrade; The temperature of sterilization is unsuitable too high, not so can cause the inactivation of the organotrophy element in the compound particles, is unfavorable for that the growth of Strains of Flammulina velutipes absorbs;
5) high-temperature sterilization being processed rear weight ratio is analysis for soybean powder and the 10%-15% Zulkovsky starch of 3%-5%, after mixing, join in the composite grain of alternanthera philoxeroides and rice straw, described alternanthera philoxeroides particle and rice straw particle are because its total amount is larger, be divided into ten addings, add 1/10th of gross weight every 10min, stirring at room was mixed 1-2h after all adding was finished, and mixed fully;
6) be the calcium superphosphate of 0.5%-1% and the sal epsom of 0.5%-1% with adding weight ratio in the mixed solid particulate, add corresponding gross weight after mixing than 2/3rds distilled water, the pH value that uses the dilute hydrochloric acid of a small amount of saturated solution of sodium bicarbonate and 0.05mol/L to regulate to mix rear substratum is to 6.5-7.0;
7) moisture with gypsum and residuals weight fully is mixed into thick liquid, joins in the mixture, in the single port plastics bag of packing into, tightens sack, low-temperature storage under the aseptic condition after light the pressure.
The actual disposition method of the culture medium for golden mushroom that described practical function of the present invention is better is:
1) the alternanthera philoxeroides live body is removed root and processed, through sunlight exposure, remove the large quantity of moisture in the alternanthera philoxeroides live body; Described sunlight exposure process can be divided into morning 8:00 to 11:00 three hours, noon 11:00 to 14:00 three hours and afternoon 14:00-17:00 three hours, the Exposure to Sunlight time of every day is 9 hours, according to the sunlight strength of different time, be chosen in the water content that 11 points, and 17 3 time points are measured respectively the alternanthera philoxeroides live body at 14;
2) will dry rear live body water content be 8% alternanthera philoxeroides stir to pulverize 1h through pulverizer, described alternanthera philoxeroides the weight of interior moisture content count the substratum weight ratio; The alternanthera philoxeroides particle that obtains 0.9cm by the filter screen screening is finished in stirring, and particle is weighed rear stand-by;
3) simultaneously the rice straw of water content 7% is also pulverized through the pulverizer stirring, described rice straw is the rice straw commonly used rice straw that oven dry makes under hot conditions behind washing immersion 3h; The stalk particle that obtains 0.7cm by the filter screen screening is finished in stirring;
4) by coagulation stirrer mixed weight than the alternanthera philoxeroides particle and the 22.5% rice straw particle that are 45%, it is divided into many groups mixes, one group the time that mixes is 1h; Improve mixing efficiency, every group is mixed the weight of alternanthera philoxeroides and the weight ratio of rice straw is 2:1, will organize mixed composite grain through the high-pressure steam sterilizing pan 18h that sterilizes more, and sterilising temp is 108 ℃;
5) high-temperature sterilization is processed after weight ratio be 3% analysis for soybean powder and 11% Zulkovsky starch, after mixing, join in the composite grain of alternanthera philoxeroides and rice straw, described alternanthera philoxeroides particle and rice straw particle are because its total amount is larger, be divided into ten addings, add 1/10th of gross weight every 10min, stirring at room was mixed 2h after all adding was finished, and mixed fully;
6) be 0.6% calcium superphosphate and 0.8% sal epsom with adding weight ratio in the mixed solid particulate, add corresponding gross weight after mixing than 2/3rds distilled water, use the dilute hydrochloric acid of a small amount of saturated solution of sodium bicarbonate and 0.05mol/L to regulate the pH value to 6.8 that mixes rear substratum;
7) gypsum and the distilled water that remains 1/3rd weight ratios fully are mixed into thick liquid, through slowly being added drop-wise in the mixture under the condition that stirs at stirrer, described thick liquid drips in 2h, pack in the single port plastics bag, leave standstill and deposit 1h, tighten sack, low-temperature storage under the aseptic condition after light the pressure.
The invention has the advantages that: the larger alternanthera philoxeroides of the environmental pollution that needle mushroom cultural method of the present invention utilizes is as the body material of substratum, stalk also belongs to the reject product in the agriculture production simultaneously, utilization of waste material, manufacturing and production cost are lower, saved production cost, improved the economic benefit of needle mushroom, needle mushroom easily absorbs and has decomposed the chemical composition of alternanthera philoxeroides in growth simultaneously, make to have comprised other nutritive ingredients that other needle mushroom did not contain on the market in the needle mushroom, improved the nutritive value of needle mushroom.
Embodiment
The present invention is described in further detail below in conjunction with embodiment.
Embodiment 1:A kind of culture medium for golden mushroom, prescription and the weight ratio of described substratum are as follows: the alternanthera philoxeroides of 40%-50%, the rice straw of 20%-25%, analysis for soybean powder 3%-5%, Zulkovsky starch 10%-15%, gypsum 1%-3%, calcium superphosphate 0.5%-1% and sal epsom 0.5%-1%.
The weight of alternanthera philoxeroides is the alternanthera philoxeroides weight after drying, and moist alternanthera philoxeroides not only is inconvenient to weigh, and easily regrows, and is unfavorable for the growth of needle mushroom.The weight ratio of described alternanthera philoxeroides and rice straw is 2:1, alternanthera philoxeroides provides Growth of Flammulina Velutipes necessary nitrogenous source, stalk provides Growth of Flammulina Velutipes required carbon source, also contain carbon, the ample supply of nitrogen element when the alternanthera philoxeroides that content is larger can guarantee Growth of Flammulina Velutipes in the alternanthera philoxeroides simultaneously.
Contain a small amount of moisture in the substratum, described water is distilled water, the weight ratio of moisture and substratum weight ratio are 1:1.5, need moisture in the growing environment of needle mushroom, very few moisture is unfavorable for the needle mushroom normal growth, too much moisture easily causes medium liquid, is unfavorable for the stable typing of substratum and the located growth of Strains of Flammulina velutipes.
After high-temperature boiling crushing, stirring 1h, the filtrate of gained after double gauze filters gets analysis for soybean powder to analysis for soybean powder after filtrate dries under aseptic condition by common soya bean; Analysis for soybean powder provides necessary amino acid for Growth of Flammulina Velutipes, but in use needs to remove bacterium wherein, prevents the somatomedin of other mushrooms plunder needle mushroom, guarantees the normal growth of needle mushroom.
Gypsum is the calcining of plaster stone process and the levigate white lime powder that forms and the mixture of plaster stone, and blending ratio is 5:1, and by add a small amount of unslaked lime in white lime, mixing with distilled water is easily to produce a small amount of heat, raising mixing efficiency and mixed effect.
Embodiment 2:The present invention uses conventional corn core substratum and alternanthera philoxeroides substratum to carry out cost this is tested, experimental result is as shown in the table: culture medium for golden mushroom of the present invention the preferred weight percent proportioning of each composition be: alternanthera philoxeroides 45%, rice straw 22.5%, analysis for soybean powder 3%, Zulkovsky starch 11%, gypsum 1.5%, calcium superphosphate 0.6%, sal epsom 0.8%, the quality per distribution ratio of the distilled water that wherein mixes is 56.2%, according to the experiment that compares of the substratum of this proportioning and daily common corn cob substratum.
Not only the corn cob substratum with common on the market is identical for the content of protein content and carbohydrate in the needle mushroom that use alternanthera philoxeroides substratum is grown in the experiment; Contain a small amount of triterpenoid saponin compounds and flavonoid compounds in the substratum of the present invention simultaneously, the triterpenoid saponin compounds can promote the metabolic function of human body, strengthens the immunizing power of human body; And the effect that has inhibition tumor cell and protectiving ultraviolet to damage is also found in the general tool step-down of flavonoid compounds, the antibiotic and effect of regulating blood battalion osmotic pressure at present, and diverse in function has improved nutritive value and the practical value of needle mushroom.
Embodiment 3:The collocation method of a kind of culture medium for golden mushroom of the present invention:
1) the alternanthera philoxeroides live body is removed root and processed, through sunlight exposure, remove the large quantity of moisture in the alternanthera philoxeroides live body; Described sunlight exposure process can be divided into morning 8:00 to 11:00 three hours, noon 11:00 to 14:00 three hours and afternoon 14:00-17:00 three hours, the Exposure to Sunlight time of every day is 9 hours, according to the sunlight strength of different time, be chosen in the water content that 11 points, and 17 3 time points are measured respectively the alternanthera philoxeroides live body at 14; Alternanthera philoxeroides could normally be used after need to drying inactivation, not so not only can affect the content of nitrogen element in the substratum, contains simultaneously activated alternanthera philoxeroides in the situation that there is the moisture existence easily to regrow;
2) will dry rear live body water content be 10%-15% alternanthera philoxeroides stir to pulverize 1h through pulverizer, described alternanthera philoxeroides the weight of interior moisture content count the substratum weight ratio; The alternanthera philoxeroides particle that obtains 0.8-1CM by the filter screen screening is finished in stirring, and particle is weighed rear stand-by; The alternanthera philoxeroides particle of this diameter makes things convenient for the adhering to of root system of needle mushroom;
3) simultaneously the rice straw of water content 5%-10% is also pulverized through the pulverizer stirring, described rice straw is the rice straw commonly used rice straw that oven dry makes under hot conditions behind washing immersion 3h; The stalk particle that obtains 0.5-0.6CM by the filter screen screening is finished in stirring; Tiny stalk particle makes things convenient for the needle mushroom root system to the absorption of its nutrition, improves growth efficiency;
4) pass through coagulation stirrer mixed weight than being the alternanthera philoxeroides particle of 40%-50% and 20%-25% rice straw particle, it is divided into many groups mixes, one group the time that mixes is 0.5-1h; Improve mixing efficiency, every group is mixed the weight of alternanthera philoxeroides and the weight ratio of rice straw is 2:1, will organize mixed composite grain through the high-pressure steam sterilizing pan 18h that sterilizes more, and sterilising temp is 100-120 degree centigrade; The temperature of sterilization is unsuitable too high, not so can cause the inactivation of the organotrophy element in the compound particles, is unfavorable for that the growth of Strains of Flammulina velutipes absorbs;
5) high-temperature sterilization being processed rear weight ratio is analysis for soybean powder and the 10%-15% Zulkovsky starch of 3%-5%, after mixing, join in the composite grain of alternanthera philoxeroides and rice straw, described alternanthera philoxeroides particle and rice straw particle are because its total amount is larger, be divided into ten addings, add 1/10th of gross weight every 10min, stirring at room was mixed 1-2h after all adding was finished, and mixed fully;
6) be the calcium superphosphate of 0.5%-1% and the sal epsom of 0.5%-1% with adding weight ratio in the mixed solid particulate, add corresponding gross weight after mixing than 2/3rds distilled water, the pH value that uses the dilute hydrochloric acid of a small amount of saturated solution of sodium bicarbonate and 0.05mol/L to regulate to mix rear substratum is to 6.5-7.0;
7) gypsum and the distilled water that remains 1/3rd weight ratios fully are mixed into thick liquid, through slowly being added drop-wise in the mixture under the condition that stirs at stirrer, described thick liquid drips in 1-2h, pack in the single port plastics bag, leave standstill and deposit 1h, tighten sack, low-temperature storage under the aseptic condition after light the pressure.
Need to prove, above-mentioned only is preferred embodiment of the present invention, is not to limit protection scope of the present invention, and the equivalents of having done on the basis of above-described embodiment all belongs to protection scope of the present invention.
Claims (10)
1. a culture medium for golden mushroom is characterized in that, prescription and the weight ratio of described substratum are as follows: the alternanthera philoxeroides of 40%-50%, the rice straw of 20%-25%, analysis for soybean powder 3%-5%, Zulkovsky starch 10%-15%, gypsum 1%-3%, calcium superphosphate 0.5%-1%, sal epsom 0.5%-1%.
2. a kind of culture medium for golden mushroom according to claim 1 is characterized in that, the weight of described alternanthera philoxeroides is the alternanthera philoxeroides weight after drying.
3. a kind of culture medium for golden mushroom according to claim 1 is characterized in that, the weight ratio of described alternanthera philoxeroides and rice straw is 2:1.
4. a kind of culture medium for golden mushroom according to claim 1 is characterized in that, contains a small amount of moisture in the described substratum, and described water is distilled water, and the weight ratio of moisture and substratum weight ratio are 1:1.5.
5. described a kind of culture medium for golden mushroom according to claim 1 is characterized in that, after high-temperature boiling crushing, stirring 1h, the filtrate of gained after double gauze filters must analysis for soybean powder after filtrate dry under aseptic condition by common soya bean for described analysis for soybean powder.
6. a kind of culture medium for golden mushroom according to claim 1 is characterized in that, described Zulkovsky starch is by one or more mixtures in sweet potato starch, Starch rice and the potato starch.
7. a kind of culture medium for golden mushroom according to claim 1 is characterized in that, described gypsum is the calcining of plaster stone process and the levigate white lime powder that forms and the mixture of plaster stone, and blending ratio is 5:1.
8. a kind of culture medium for golden mushroom according to claim 1, it is characterized in that, described culture medium for golden mushroom the preferred weight percent proportioning of each composition be: alternanthera philoxeroides 45%, rice straw 22.5%, analysis for soybean powder 3%, Zulkovsky starch 11%, gypsum 1.5%, calcium superphosphate 0.6%, sal epsom 0.8%, the quality per distribution ratio of the distilled water that wherein mixes is 56.2%.
9. the collocation method of a culture medium for golden mushroom:
1) the alternanthera philoxeroides live body is removed root and processed, through sunlight exposure, remove the large quantity of moisture in the alternanthera philoxeroides live body; Described sunlight exposure process can be divided into morning 8:00 to 11:00 three hours, noon 11:00 to 14:00 three hours and afternoon 14:00-17:00 three hours, the Exposure to Sunlight time of every day is 9 hours, according to the sunlight strength of different time, be chosen in the water content that 11 points, and 17 3 time points are measured respectively the alternanthera philoxeroides live body at 14;
2) will dry rear live body water content be 10%-15% alternanthera philoxeroides stir to pulverize 1h through pulverizer, described alternanthera philoxeroides the weight of interior moisture content count the substratum weight ratio; The alternanthera philoxeroides particle that obtains 0.8-1CM by the filter screen screening is finished in stirring, and particle is weighed rear stand-by;
3) simultaneously the rice straw of water content 5%-10% is also pulverized through the pulverizer stirring, described rice straw is the rice straw commonly used rice straw that oven dry makes under hot conditions behind washing immersion 3h; The stalk particle that obtains 0.5-0.6CM by the filter screen screening is finished in stirring;
4) pass through coagulation stirrer mixed weight than being the alternanthera philoxeroides particle of 40%-50% and 20%-25% rice straw particle, it is divided into many groups mixes, one group the time that mixes is 0.5-1h; Improve mixing efficiency, every group is mixed the weight of alternanthera philoxeroides and the weight ratio of rice straw is 2:1, will organize mixed composite grain through the high-pressure steam sterilizing pan 18h that sterilizes more, and sterilising temp is 100-120 degree centigrade;
5) high-temperature sterilization being processed rear weight ratio is analysis for soybean powder and the 10%-15% Zulkovsky starch of 3%-5%, after mixing, join in the composite grain of alternanthera philoxeroides and rice straw, described alternanthera philoxeroides particle and rice straw particle are because its total amount is larger, be divided into ten addings, add 1/10th of gross weight every 10min, stirring at room was mixed 1-2h after all adding was finished, and mixed fully;
6) be the calcium superphosphate of 0.5%-1% and the sal epsom of 0.5%-1% with adding weight ratio in the mixed solid particulate, add corresponding gross weight after mixing than 2/3rds distilled water, the pH value that uses the dilute hydrochloric acid of a small amount of saturated solution of sodium bicarbonate and 0.05mol/L to regulate to mix rear substratum is to 6.5-7.0;
7) gypsum and the distilled water that remains 1/3rd weight ratios fully are mixed into thick liquid, through slowly being added drop-wise in the mixture under the condition that stirs at stirrer, described thick liquid drips in 1-2h, pack in the single port plastics bag, leave standstill and deposit 1h, tighten sack, low-temperature storage under the aseptic condition after light the pressure.
10. the collocation method of a kind of culture medium for golden mushroom according to claim 9: it is characterized in that, the better collocation method of described practical function is:
1) the alternanthera philoxeroides live body is removed root and processed, through sunlight exposure, remove the large quantity of moisture in the alternanthera philoxeroides live body; Described sunlight exposure process can be divided into morning 8:00 to 11:00 three hours, noon 11:00 to 14:00 three hours and afternoon 14:00-17:00 three hours, the Exposure to Sunlight time of every day is 9 hours, according to the sunlight strength of different time, be chosen in the water content that 11 points, and 17 3 time points are measured respectively the alternanthera philoxeroides live body at 14;
2) will dry rear live body water content be 8% alternanthera philoxeroides stir to pulverize 1h through pulverizer, described alternanthera philoxeroides the weight of interior moisture content count the substratum weight ratio; The alternanthera philoxeroides particle that obtains 0.9cm by the filter screen screening is finished in stirring, and particle is weighed rear stand-by;
3) simultaneously the rice straw of water content 7% is also pulverized through the pulverizer stirring, described rice straw is the rice straw commonly used rice straw that oven dry makes under hot conditions behind washing immersion 3h; The stalk particle that obtains 0.7cm by the filter screen screening is finished in stirring;
4) by coagulation stirrer mixed weight than the alternanthera philoxeroides particle and the 22.5% rice straw particle that are 45%, it is divided into many groups mixes, one group the time that mixes is 1h; Improve mixing efficiency, every group is mixed the weight of alternanthera philoxeroides and the weight ratio of rice straw is 2:1, will organize mixed composite grain through the high-pressure steam sterilizing pan 18h that sterilizes more, and sterilising temp is 108 degrees centigrade;
5) high-temperature sterilization is processed after weight ratio be 3% analysis for soybean powder and 11% Zulkovsky starch, after mixing, join in the composite grain of alternanthera philoxeroides and rice straw, described alternanthera philoxeroides particle and rice straw particle are because its total amount is larger, be divided into ten addings, add 1/10th of gross weight every 10min, stirring at room was mixed 2h after all adding was finished, and mixed fully;
6) be 0.6% calcium superphosphate and 0.8% sal epsom with adding weight ratio in the mixed solid particulate, add corresponding gross weight after mixing than 2/3rds distilled water, use the dilute hydrochloric acid of a small amount of saturated solution of sodium bicarbonate and 0.05mol/L to regulate the pH value to 6.8 that mixes rear substratum;
7) gypsum and the distilled water that remains 1/3rd weight ratios fully are mixed into thick liquid, through slowly being added drop-wise in the mixture under the condition that stirs at stirrer, described thick liquid drips in 2h, pack in the single port plastics bag, leave standstill and deposit 1h, tighten sack, low-temperature storage under the aseptic condition after light the pressure.
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CN103524233A (en) * | 2013-10-17 | 2014-01-22 | 安徽徽王食品有限公司 | Flammulina velutipes cultivation material prepared from dry straw and preparation method thereof |
CN103553803A (en) * | 2013-11-01 | 2014-02-05 | 合肥市财锦食品有限公司 | Flammulina velutipes cultivation material taking beanstalk dust as raw material and preparation method thereof |
CN104396573A (en) * | 2014-12-19 | 2015-03-11 | 苏州市经纬农产品有限公司 | Flammulina velutipes cultivation method |
CN105418268A (en) * | 2015-12-25 | 2016-03-23 | 遵义市明华福食用菌种植场 | Culture medium and culturing method for flammulina velutipes |
CN109105155A (en) * | 2018-11-07 | 2019-01-01 | 广西仁泰生物科技有限公司 | A kind of implantation methods of tea tree interplanting needle mushroom |
CN112088721A (en) * | 2020-10-22 | 2020-12-18 | 百色学院 | Culture medium and culture method for culturing edible fungi by using alternanthera philoxeroides |
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CN103524233A (en) * | 2013-10-17 | 2014-01-22 | 安徽徽王食品有限公司 | Flammulina velutipes cultivation material prepared from dry straw and preparation method thereof |
CN103553803A (en) * | 2013-11-01 | 2014-02-05 | 合肥市财锦食品有限公司 | Flammulina velutipes cultivation material taking beanstalk dust as raw material and preparation method thereof |
CN104396573A (en) * | 2014-12-19 | 2015-03-11 | 苏州市经纬农产品有限公司 | Flammulina velutipes cultivation method |
CN105418268A (en) * | 2015-12-25 | 2016-03-23 | 遵义市明华福食用菌种植场 | Culture medium and culturing method for flammulina velutipes |
CN109105155A (en) * | 2018-11-07 | 2019-01-01 | 广西仁泰生物科技有限公司 | A kind of implantation methods of tea tree interplanting needle mushroom |
CN112088721A (en) * | 2020-10-22 | 2020-12-18 | 百色学院 | Culture medium and culture method for culturing edible fungi by using alternanthera philoxeroides |
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