CN103333019B - Practical collybia radicata culture medium and preparation method thereof - Google Patents
Practical collybia radicata culture medium and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a practical collybia radicata culture medium. The culture medium comprises the following formulas in percent by weight: 24% to 28% of corncob, 20% to 25 % of groundnut meal, 10% to 15% of mulberry leaf, 3% to 5% of glucose powder, 5% to 8% of plant ash, 2% to 4% of pumpkin peel, 3% to 5% of calcium carbonate and 1% to 3% of plaster. The growing period of the culture medium, provided by the invention, is shorter than that of other culture medium with different formula ratios, the thalli grows prosperously, the average diameter of the thalli is longer, the length of the stipe is longer, the nutritive value of the thalli such as vitamin B1 content is higher, the carbohydrate and fat content is lower, meanwhile, the biological efficiency of the culture medium is high, so that the cost is saved, the economic benefit of the collybia radicata culture medium is improved, and the practical collybia radicata culture medium is suitable for large-scale popularization and production.
Description
Technical field
The present invention relates to the cultivation field of Oudemansiella Radicata, particularly relate to a kind of practical collybia radicata culture medium and collocation method thereof.
Background technology
Oudemansiella Radicata has another name called long root oudemansiella radicata, large hair grass bacterium, long root money bacterium, dew chicken.Be subordinate to Basidiomycotina, Hymenomycetes, Agaricales, Bai Mo section, Genus Oudeman Siella Spec.Oudemansiella Radicata be edible in top grade, endoplasm is delicate, handle is crisp good to eat, and rich in proteins, amino acid, fat, carbohydrate, VITAMIN and trace element components, edibleness is high.
The formula that collybia radicata culture medium conventional on the market at present adopts is: cotton seed hulls 35%, wheat skin 25%, hardwood sawdust 38%, sugar and calcium carbonate each 1%, water content 68%, the nutritive element of this substratum is comparatively single, protein content in the Oudemansiella Radicata produced is relatively low, cap is shorter, nutritive element simultaneously in thalline is less, and reason is that the nutritive substance in cotton seed hulls and wood chip joins in substratum after untreated, is unfavorable for the absorption of Oudemansiella Radicata root system, thus causing being of low nutritive value of Oudemansiella Radicata, the economic benefit of plantation is not high.
Summary of the invention
For above-mentioned Problems existing, the object of the invention is the collybia radicata culture medium and the collocation method thereof that provide a kind of good practical effect.
In order to achieve the above object, the technical solution used in the present invention is as follows: a kind of practical collybia radicata culture medium, formula and the weight ratio of described substratum are as follows: 24%-28% corn cob, the groundnut meal of 20%-25%, the mulberry leaves of 10%-15%, the glucose powder of 3%-5%, the plant ash of 5%-8%, the pumpkin capsule of 2%-4%, the calcium carbonate of 3%-5%, the gypsum of 1%-3%.
Corn cob of the present invention is corn ear gained lightweight stem stalk after degranulation process, and after described lightweight stem stalk stirs pulverization process, the particle diameter of described corn cob is 0.1-0.5cm.
Groundnut meal of the present invention is the tankage in peanut oil production process, and then and granular mill dry by high-temperature sterilization stirs rear gained, and the particle diameter of described groundnut meal is 0.1-0.3cm.
Containing moisture in substratum of the present invention, described water is the distilled water that household tap water obtains after high-temperature boiling 15-20min, and the weight ratio of described distilled water is 7:5 with substratum gross weight ratio.
Mulberry leaves of the present invention is after fresh mulberry leaves entered solarization dry-cure, and water content is 10%-15%, and described pumpkin capsule drops into fermentative processing in fermentation vat with mulberry leaves after mixing, and the mass ratio of described mulberry leaves and pumpkin capsule is 4:1.
Plant ash of the present invention is the pressed powder of gained after straw used in everyday or wheat stalk burn, the gained after the screen cloth screening that vibrating machine and aperture are 0.05cm of the pressed powder after described burning.
Gypsum of the present invention is the mixed powder that plaster stone passes through calcining and levigate white lime powder and unslaked lime, and described mixed powder is 7-10mm through the mean particle dia that mesh structure filters out.
The various composition of practical collybia radicata culture medium of the present invention preferred weight percent proportioning is as follows: 25% corn cob, the groundnut meal of 25%, the mulberry leaves of 12%, the glucose powder of 5%, the plant ash of 8%, the pumpkin capsule of 3%, the calcium carbonate of 2%, the gypsum of 2%, the percent mass proportioning of the distilled water used in described configuration substratum is 114.8%.
The collocation method of a kind of practical collybia radicata culture medium of the present invention:
1) by the corn ear buied on the market through Exposure to Sunlight process, the Exposure to Sunlight time is the 9:00-16:00 of every day, the Exposure to Sunlight time is 10-15 days, the 16:00 of every day measures the water content of corn ear, when the water content of corn ear is 5%-8% time, corn ear after drying is carried out degranulation process by excellent degranulation machine to corn ear, the corn cob obtained after degranulation is stirred by pulverizer and pulverizes, grinding time 30min, corn cob granule after pulverizing by 0.5cm aperture filter screen structure filter, it is stand-by after 0.1-0.5cm weighs for getting particle diameter;
2) the tankage peanut cake of peanut oil is bundled by large-scale fabric packing bag wrapping, packing bag is put into clear water immersion treatment, soak time is 30min, the clear water of immersion is changed after 30 minutes, to the clear water soaked without obvious greasy dirt, after immersion completes, open a parcel, pass through high-temp steam sterilizing, sterilising temp 120-150 DEG C, sterilizing completes post-drying, bake out temperature 60-80 DEG C, carry out stirring to it under sterile conditions to pulverize, peanut cake particle after pulverizing by 0.3cm aperture filter screen structure filter, it is stand-by after 0.1-0.3 weighs for getting particle diameter
3) by mulberry leaves by Exposure to Sunlight process, the Exposure to Sunlight time is the 9:00-16:00 of every day, the Exposure to Sunlight time is 3-4 days, water content after Exposure to Sunlight is 10%-15%, be that 10%-15% mulberry leaves adds in pulverizer to stir and pulverizes by the weight ratio after drying, pumpkin capsule discarded when weight ratio is 2%-4% daily cleaning pumpkin internal seeds is added while stirring, crushing, stirring 2h, mixing fully, by composite grain pack wrapping, be suspended in fermentation vat by the composite grain after pack and carry out fermentative processing, fermentation time is 2-3 days, after having fermented, deepfreeze;
4) in stirrer, the groundnut meal that weight ratio is 24%-28% corn cob and 20%-25% is added, under the condition stirred, slowly the mulberry leaves of deepfreeze and pumpkin capsule granular mixture are joined in system, add in 10min, 1h is uniformly mixed, to ensure sufficient mixed effect after having added;
5) mixed mixture is divided into many groups, many groups drop into high-pressure steam sterilizing pan sterilizing 12h simultaneously, and improve sterilizing efficiency, sterilising temp is 110 DEG C-120 DEG C, after sterilizing completes, and deposits under aseptic condition;
6) be the glucose powder of 3%-5% by weight ratio, adding weight ratio is the distilled water of gross weight than 1/3rd, is uniformly mixed 1h, the Autoclave putting into low temperature after having mixed carries out sterilizing, sterilising temp 1-3 DEG C, sterilization time 24h, put into dropping funnel after sterilizing completes stand-by;
7) mixed weight is than being the plant ash of 5%-8% and the calcium carbonate powders of 3%-5%, and being uniformly mixed 20min, after having mixed, is the filter screen structure of 0.05cm by aperture;
8) the blended solid powder after sterilizing is uniformly mixed, in stirrer, the mixed powder that weight ratio is the plant ash of 5%-8% and the calcium carbonate of 3%-5% is added while stirring, after being uniformly mixed 10min, add the distilled water of gross weight than 1/3rd, while being uniformly mixed, the dilute hydrochloric acid of a small amount of saturated solution of sodium bicarbonate and 0.02mol/L is used to regulate the PH to 6.8-7.2 of mixed culture medium;
9) be that the gypsum of 1%-3% and the distilled water of residue 1/3rd weight ratios are fully mixed into milky white liquid by weight ratio, be uniformly mixed 20min;
10) slowly being joined by milky white liquid under the condition that large-scale stirrer slowly stirs regulates in the solid culture based mixtures of PH, while dripping, the glucose solution in dropping funnel is slowly instilled in substratum, dropwise in 30min, after being added dropwise to complete, substratum divides and is assembled in multiple single port plastics bag by speed, leave standstill and deposit 3h, after light pressure, tighten sack, low-temperature storage under aseptic condition.
The invention has the advantages that: the substratum of Oudemansiella Radicata of the present invention is the main body carbon source for substratum with corn cob, secondary carbon source using mulberry leaves as substratum, described corn cob inner pore is larger, Mierocrystalline cellulose is easier to decompose, monose after cellulose decomposition and polysaccharide, Oudemansiella Radicata is easier to absorb, contain various saccharides material in mulberry leaves simultaneously, compensate the deficiency of glucide in corn cob, corn cob granule can assist the stable sizing of substratum simultaneously, improve the ventilation property of substratum, facilitate the respiration of Oudemansiella Radicata root system; Simultaneously using groundnut meal as the nitrogenous source of substratum, secondary carbon source using pumpkin capsule as substratum, groundnut meal is again after process of deoiling, inner containing a large amount of protein and amino acid, contain the amino acid do not had in groundnut meal in pumpkin capsule simultaneously, compensate amino acid whose deficiency in groundnut meal, facilitate the absorption of thalline, the amino acid of polishing improves synthesis and the content of the middle protein of Oudemansiella Radicata, improves the output of Oudemansiella Radicata simultaneously; Selected plant ash and calcium carbonate as the inorganic salt raw material of substratum simultaneously, and be not only the inorganic salt that substratum provides necessary, stablizing of substratum can be assisted shaping simultaneously; Select sucrose as the auxiliary material of substratum, ensure that local substratum when nutritive element under-supply inadequate, ensure that the normal growth of Oudemansiella Radicata, improve Oudemansiella Radicata growth time resistance, bacterial strain is sturdy not easily to lodge; The particle of the material that the present invention selects is moderate, facilitates between raw material and mixes, and facilitates the fixing of careless Root Absorption and root system.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
embodiment 1:a kind of practical collybia radicata culture medium, formula and the weight ratio of described substratum are as follows: 25% corn cob, the groundnut meal of 25%, the mulberry leaves of 12%, the glucose powder of 5%, the plant ash of 8%, the pumpkin capsule of 3%, the calcium carbonate of 2%, the gypsum of 2%, the percent mass proportioning of the distilled water used in described configuration substratum is 114.8%.
embodiment 2:a kind of practical collybia radicata culture medium, formula and the weight ratio of described substratum are as follows: 24% corn cob, the groundnut meal of 20%, the mulberry leaves of 10%, the glucose powder of 3%, the plant ash of 5%, the pumpkin capsule of 2.5%, the calcium carbonate of 3%, the gypsum of 1%; The percent mass proportioning of the distilled water used in described configuration substratum is 95.9%.
embodiment 3:a kind of practical collybia radicata culture medium, formula and the weight ratio of described substratum are as follows: 28% corn cob, the groundnut meal of 25%, the mulberry leaves of 15%, the glucose powder of 5%, the plant ash of 8%, the pumpkin capsule of 3%, the calcium carbonate of 5%, the gypsum of 3%; The percent mass proportioning of the distilled water used in described configuration substratum is 128.8%.
embodiment 4:a kind of practical collybia radicata culture medium, formula and the weight ratio of described substratum are as follows: 26% corn cob, the groundnut meal of 22%, the mulberry leaves of 11%, the glucose powder of 4%, the plant ash of 6%, the pumpkin capsule of 4%, the calcium carbonate of 4%, the gypsum of 3%; The percent mass proportioning of the distilled water used in described configuration substratum is 112%.
embodiment 5:as shown in the table to the comparative test result of embodiment 4 to be the formulated substratum of body material be body material carries out Oudemansiella Radicata cultivation to embodiment 1:
The substratum be made into according to this proportioning and the contrast experiment of ordinary culture medium, the proportion optimizing of described substratum is as follows: 25% corn cob, the groundnut meal of 25%, the mulberry leaves of 12%, the glucose powder of 5%, the plant ash of 8%, the pumpkin capsule of 3%, the calcium carbonate of 2%, the gypsum of 2%, the percent mass proportioning of the distilled water used in described configuration substratum is 114.8%; The substratum that be shorter than other proportionings of substratum on processing maturation period of the embodiment 1 shown in table, thalli growth is vigorous, the mean diameter of cap is larger, stem length is longer, and the content of nutritive value as VITMAIN B1 of thalline is compared height, and carbohydrate and fatty content are compared low simultaneously, the biological transformation ratio of this substratum is high simultaneously, cost-saving, improve the economic benefit that Oudemansiella Radicata is cultivated, be applicable to large-scale promotion and produce.
embodiment 6:the collocation method of a kind of practical collybia radicata culture medium of the present invention:
1) by the corn ear buied on the market through Exposure to Sunlight process, the Exposure to Sunlight time is the 9:00-16:00 of every day, the Exposure to Sunlight time is 10-15 days, the 16:00 of every day measures the water content of corn ear, when the water content of corn ear is 5%-8% time, corn ear after drying is carried out degranulation process by excellent degranulation machine to corn ear, the corn cob obtained after degranulation is stirred by pulverizer and pulverizes, grinding time 30min, corn cob granule after pulverizing by 0.5cm aperture filter screen structure filter, it is stand-by after 0.1-0.5cm weighs for getting particle diameter;
2) the tankage peanut cake of peanut oil is bundled by large-scale fabric packing bag wrapping, packing bag is put into clear water immersion treatment, soak time is 30min, the clear water of immersion is changed after 30 minutes, to the clear water soaked without obvious greasy dirt, after immersion completes, open a parcel, pass through high-temp steam sterilizing, sterilising temp 120-150 DEG C, sterilizing completes post-drying, bake out temperature 60-80 DEG C, carry out stirring to it under sterile conditions to pulverize, peanut cake particle after pulverizing by 0.3cm aperture filter screen structure filter, it is stand-by after 0.1-0.3 weighs for getting particle diameter
3) by mulberry leaves by Exposure to Sunlight process, the Exposure to Sunlight time is the 9:00-16:00 of every day, the Exposure to Sunlight time is 3-4 days, water content after Exposure to Sunlight is 10%-15%, be that 10%-15% mulberry leaves adds in pulverizer to stir and pulverizes by the weight ratio after drying, pumpkin capsule discarded when weight ratio is 2%-3% daily cleaning pumpkin internal seeds is added while stirring, crushing, stirring 2h, mixing fully, by composite grain pack wrapping, be suspended in fermentation vat by the composite grain after pack and carry out fermentative processing, fermentation time is 2-3 days, after having fermented, deepfreeze;
4) in stirrer, the groundnut meal that weight ratio is 24%-28% corn cob and 20%-25% is added, under the condition stirred, slowly the mulberry leaves of deepfreeze and pumpkin capsule granular mixture are joined in system, add in 10min, 1h is uniformly mixed, to ensure sufficient mixed effect after having added;
5) mixed mixture is divided into many groups, many groups drop into high-pressure steam sterilizing pan sterilizing 12h simultaneously, and improve sterilizing efficiency, sterilising temp is 110 DEG C-120 DEG C, after sterilizing completes, and deposits under aseptic condition;
6) be the glucose powder of 3%-5% by weight ratio, adding weight ratio is the distilled water of gross weight than 1/3rd, is uniformly mixed 1h, the Autoclave putting into low temperature after having mixed carries out sterilizing, sterilising temp 1-3 DEG C, sterilization time 24h, put into dropping funnel after sterilizing completes stand-by;
7) mixed weight is than being the plant ash of 5%-8% and the calcium carbonate powders of 3%-5%, and being uniformly mixed 20min, after having mixed, is the filter screen structure of 0.05cm by aperture;
8) the blended solid powder after sterilizing is uniformly mixed, in stirrer, the mixed powder that weight ratio is the plant ash of 5%-8% and the calcium carbonate of 3%-5% is added while stirring, after being uniformly mixed 10min, add the distilled water of gross weight than 1/3rd, while being uniformly mixed, the dilute hydrochloric acid of a small amount of saturated solution of sodium bicarbonate and 0.02mol/L is used to regulate the PH to 6.8-7.2 of mixed culture medium;
9) be that the gypsum of 1%-3% and the distilled water of residue 1/3rd weight ratios are fully mixed into milky white liquid by weight ratio, be uniformly mixed 20min;
10) slowly being joined by milky white liquid under the condition that large-scale stirrer slowly stirs regulates in the solid culture based mixtures of PH, while dripping, the glucose solution in dropping funnel is slowly instilled in substratum, dropwise in 30min, after being added dropwise to complete, substratum divides and is assembled in multiple single port plastics bag by speed, leave standstill and deposit 3h, after light pressure, tighten sack, low-temperature storage under aseptic condition.
It should be noted that, above-mentioned is only preferred embodiment of the present invention, is not used for limiting protection scope of the present invention, and equivalents done on the basis of above-described embodiment all belongs to protection scope of the present invention.
Claims (5)
1. the collocation method of a practical collybia radicata culture medium, it is characterized in that, formula and the weight ratio of described substratum are as follows: 24%-28% corn cob, the groundnut meal of 20%-25%, the mulberry leaves of 10%-15%, the glucose powder of 3%-5%, the plant ash of 5%-8%, the pumpkin capsule of 2%-4%, the calcium carbonate of 3%-5%, the gypsum of 1%-3%; Containing moisture in described substratum, described water is the distilled water that household tap water obtains after high-temperature boiling 15-20min, and the weight ratio of described distilled water is 7:5 with substratum gross weight ratio; The collocation method of described a kind of practical collybia radicata culture medium:
1) by the corn ear buied on the market through Exposure to Sunlight process, the Exposure to Sunlight time is the 9:00-16:00 of every day, the Exposure to Sunlight time is 10-15 days, the 16:00 of every day measures the water content of corn ear, when the water content of corn ear is 5%-8% time, corn ear after drying is carried out degranulation process by excellent degranulation machine to corn ear, the corn cob obtained after degranulation is stirred by pulverizer and pulverizes, grinding time 30min, corn cob granule after pulverizing by 0.5cm aperture filter screen structure filter, it is stand-by after 0.1-0.5cm weighs for getting particle diameter;
2) the tankage peanut cake of peanut oil is bundled by large-scale fabric packing bag wrapping, packing bag is put into clear water immersion treatment, soak time is 30min, the clear water of immersion is changed after 30 minutes, to the clear water soaked without obvious greasy dirt, after immersion completes, open a parcel, pass through high-temp steam sterilizing, sterilising temp 120-150 DEG C, sterilizing completes post-drying, bake out temperature 60-80 DEG C, carry out stirring to it under sterile conditions to pulverize, peanut cake particle after pulverizing by 0.3cm aperture filter screen structure filter, it is stand-by after 0.1-0.3cm weighs for getting particle diameter
3) by mulberry leaves by Exposure to Sunlight process, the Exposure to Sunlight time is the 9:00-16:00 of every day, the Exposure to Sunlight time is 3-4 days, water content after Exposure to Sunlight is 10%-15%, be that 10%-15% mulberry leaves adds in pulverizer to stir and pulverizes by the weight ratio after drying, pumpkin capsule discarded when weight ratio is 2%-4% daily cleaning pumpkin internal seeds is added while stirring, crushing, stirring 2h, mixing fully, by composite grain pack wrapping, be suspended in fermentation vat by the composite grain after pack and carry out fermentative processing, fermentation time is 2-3 days, after having fermented, deepfreeze;
4) in stirrer, the groundnut meal that weight ratio is 24%-28% corn cob and 20%-25% is added, under the condition stirred, slowly the mulberry leaves of deepfreeze and pumpkin capsule granular mixture are joined in system, add in 10min, 1h is uniformly mixed, to ensure sufficient mixed effect after having added;
5) mixed mixture is divided into many groups, many groups drop into high-pressure steam sterilizing pan sterilizing 12h simultaneously, and improve sterilizing efficiency, sterilising temp is 110 DEG C-120 DEG C, after sterilizing completes, and deposits under aseptic condition;
6) be the glucose powder of 3%-5% by weight ratio, adding weight ratio is the distilled water of gross weight than 1/3rd, is uniformly mixed 1h, the Autoclave putting into low temperature after having mixed carries out sterilizing, sterilising temp 1-3 DEG C, sterilization time 24h, put into dropping funnel after sterilizing completes stand-by;
7) mixed weight is than being the plant ash of 5%-8% and the calcium carbonate powders of 3%-5%, and being uniformly mixed 20min, after having mixed, is the filter screen structure of 0.05cm by aperture;
8) the blended solid powder after sterilizing is uniformly mixed, in stirrer, the mixed powder that weight ratio is the plant ash of 5%-8% and the calcium carbonate of 3%-5% is added while stirring, after being uniformly mixed 10min, add the distilled water of gross weight than 1/3rd, while being uniformly mixed, the dilute hydrochloric acid of a small amount of saturated solution of sodium bicarbonate and 0.02mol/L is used to regulate the pH to 6.8-7.2 of mixed culture medium;
9) be that the gypsum of 1%-3% and the distilled water of residue 1/3rd weight ratios are fully mixed into milky white liquid by weight ratio, be uniformly mixed 20min;
10) slowly being joined by milky white liquid under the condition that large-scale stirrer slowly stirs regulates in the solid culture based mixtures of pH, while dripping, the glucose solution in dropping funnel is slowly instilled in substratum, dropwise in 30min, after being added dropwise to complete, substratum is divided and is assembled in single port plastics bag, leave standstill and deposit 3h, after light pressure, tighten sack, low-temperature storage under aseptic condition.
2. the collocation method of practical collybia radicata culture medium according to claim 1, it is characterized in that, described mulberry leaves is after fresh mulberry leaves entered solarization dry-cure, water content is 10%-15%, described pumpkin capsule drops into fermentative processing in fermentation vat with mulberry leaves after mixing, and the mass ratio of described mulberry leaves and pumpkin capsule is 4:1.
3. the collocation method of practical collybia radicata culture medium according to claim 1, it is characterized in that, described plant ash is the pressed powder of gained after straw used in everyday or wheat stalk burn, the gained after the screen cloth screening that vibrating machine and aperture are 0.05cm of the pressed powder after described burning.
4. the collocation method of practical collybia radicata culture medium according to claim 1, it is characterized in that, described gypsum is the mixed powder that plaster stone passes through calcining and levigate white lime powder and unslaked lime, and described mixed powder is 7-10mm through the mean particle dia that mesh structure filters out.
5. the collocation method of practical collybia radicata culture medium according to claim 1, it is characterized in that, the various composition of described practical collybia radicata culture medium preferred weight percent proportioning is as follows: 25% corn cob, the groundnut meal of 25%, the mulberry leaves of 12%, the glucose powder of 5%, the plant ash of 8%, the pumpkin capsule of 3%, the calcium carbonate of 2%, the gypsum of 2%, the percent mass proportioning of the distilled water used in described configuration substratum is 114.8%.
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| CN103725614A (en) * | 2013-12-18 | 2014-04-16 | 达基服装辅料（南通）有限公司 | Beef soup culture medium |
| EA027426B1 (en) * | 2015-03-05 | 2017-07-31 | Общество С Ограниченной Ответственностью "Нереус Про" | Granulated agricultural hydrogel that accumulates and releases moisture and nutritient substances, and production and application thereof |
| CN104945153A (en) * | 2015-07-06 | 2015-09-30 | 合肥福泉现代农业科技有限公司 | Collybia radicata efficient culture medium with long-term infectious microbe inhibiting effect and preparation method |
| CN105130638A (en) * | 2015-08-27 | 2015-12-09 | 马鞍山市安康菌业有限公司 | Efficient oudemansiella radicata culture medium with propolis being antibacterial and nutrient substances and preparation method therefor |
| CN107434701A (en) * | 2016-05-26 | 2017-12-05 | 桂林洁宇环保科技有限责任公司 | A kind of new long root mushroom planting material |
| CN107434453A (en) * | 2016-05-26 | 2017-12-05 | 秦家运 | A kind of planting material for improving long root mushroom yield |
| CN107434732A (en) * | 2016-05-27 | 2017-12-05 | 桂林宏泰养殖农民专业合作社 | A kind of carbon source is the long root mushroom compost of shoot vegetable root corncob |
| CN107434559A (en) * | 2016-05-27 | 2017-12-05 | 桂林宏泰养殖农民专业合作社 | The long root mushroom high-yield culturing material that a kind of corncob pueraria root residue is configured to |
| CN108371056A (en) * | 2018-03-21 | 2018-08-07 | 潍坊医学院 | A kind of chrysanthemum culture medium |
| CN110972810A (en) * | 2019-12-20 | 2020-04-10 | 枞遍天下(山东)农业科技有限公司 | Planting method for black collybia albuminosa edible fungi for avoiding infectious microbe infection |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6565860B1 (en) * | 1999-05-14 | 2003-05-20 | Jay-Mar, Inc. | Surfactant coated products and methods for their use in promoting plant growth and soil remediation |
| CN102276314A (en) * | 2010-06-13 | 2011-12-14 | 李畅 | Preparation method of blended nutrient for oudemanciella radicata |
| CN102511312A (en) * | 2011-12-27 | 2012-06-27 | 天津市林业果树研究所 | Method for processing cultivation material for edible fungi |
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| JP2007169096A (en) * | 2005-12-20 | 2007-07-05 | Kiyohiro Maeda | Plant-based compost and method for growing farm product and farm product |
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2013
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6565860B1 (en) * | 1999-05-14 | 2003-05-20 | Jay-Mar, Inc. | Surfactant coated products and methods for their use in promoting plant growth and soil remediation |
| CN102276314A (en) * | 2010-06-13 | 2011-12-14 | 李畅 | Preparation method of blended nutrient for oudemanciella radicata |
| CN102511312A (en) * | 2011-12-27 | 2012-06-27 | 天津市林业果树研究所 | Method for processing cultivation material for edible fungi |
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