CN105130638A - Efficient oudemansiella radicata culture medium with propolis being antibacterial and nutrient substances and preparation method therefor - Google Patents

Abstract

The present invention discloses an efficient oudemansiella radicata culture medium with propolis being antibacterial and nutrient substances. The culture medium comprises the following components in parts by weight: macroporous thermally-degradable water-soluble polyvinyl alcohol microspheres, porous ultra-high-strength activated carbon microspheres, porous chitosan microspheres, rice roots, perilla oil cakes, germinated brown rice, castor oil, Hedysari polysaccharide, ionic liquid emulsified propolis and yeast. According to the culture medium provided by the present invention, porous ultra-high-strength activated carbon microspheres, porous chitosan microspheres and ionic liquid emulsified propolis are used to prepare the modified latex which is used as nutrient and antibacterial substances added into the culture medium, so that nutritive and antimicrobial effects of the culture medium are improved, thereby achieving natural and environmentally-friendly features. Macroporous thermally-degradable water-soluble polyvinyl alcohol microspheres are used to adsorb the loaded yeast for fermentation, so that the fermentation rate is effectively adjusted; decomposition of the fermentable substrate is promoted; nutrients of the culture medium are more abundant, more balanced and more convenient for absorption and utilization by the oudemansiella radicata; and a basis is provided for efficient cultivation of the oudemansiella radicata.

Description

A kind of propolis that adopts is as Oudemansiella Radicata efficient culture medium of the antibacterial and dual material of nutrition and preparation method thereof

Technical field

The present invention relates to culture medium of edible fungus field, particularly relate to a kind of propolis that adopts as Oudemansiella Radicata efficient culture medium of the antibacterial and dual material of nutrition and preparation method thereof.

Background technology

Oudemansiella Radicata is subordinate to Basidiomycota, agaric guiding principle, Agaricales, is top grade in edible mushrooms, and quality is delicate, and handle is crisp good to eat, and rich in proteins, amino acid, fat, carbohydrate, VITAMIN and trace element, edibleness is high.The Efficient Cultivation realizing Oudemansiella Radicata is significant thing.

Propolis is described as " purple gold " at European Ei, it contains above the average age for marriage flavonoid, terpene, multiple organic acid, there is significantly antibacterial, antiviral bioactive ingredients, its effective constituent breaks up bacteria growing inhibiting by histocyte, destroy the order state of cytolemma and endochylema, cause the synthesis of part bacteriolyze and anti-bacteria protein.Propolis emulsion has good fungistatic effect, and propolis emulsion particle diameter is little, and in addition, propolis emulsion is by having higher nutritive value.

The micropore of the microballoon of vesicular structure is covered with whole microballoon and gos deep into center by surface, time as carrier, have, adsorption site is many, the features such as large specific surface area and strong adsorptive power, can play and change by features such as object carrier solvability, releasabilities, through being often used as the carrier of slow release fertilizer; In the preparation process of the submerged fermentation efficient culture medium of edible fungus culturing, the decomposition of culture medium raw material and fermentation directly decide the quality of substratum, and then determine that cultivation uses the effect of gold; The control of relevant enzyme and zymophyte activity is key and the difficult point of preparation submerged fermentation efficient culture medium, do not have the report of microballoon as the control of relevant enzyme and zymophyte activity in substratum making processes of vesicular structure at present, but according to the large specific surface area of porous microsphere, be conducive to sticking in a large number of cell, being conducive to the transmission of nutritive substance and the discharge of metabolic substd and this character of intercellular interaction, should be the control that can be used to enzyme and zymophyte activity.

Summary of the invention

The Efficient Cultivation demand of Oudemansiella Radicata of the present invention, provides a kind of propolis that adopts as Oudemansiella Radicata efficient culture medium of the antibacterial and dual material of nutrition and preparation method thereof.

The present invention is achieved by the following technical solutions:

A kind of propolis that adopts is as antibacterial and Oudemansiella Radicata efficient culture medium that the is dual material of nutrition, comprise following parts by weight of component: thermal destruction water-soluble poval macroporous microsphere 3-4, superstrength porous microsphere active carbon 3-4, Porous Chitosan Microspheres 3-4, peanut pomace 25-26, Chinese torreya pomace 16-17, rice root 21-22, purple perilla oil meal 24-26, sesame seed meal 27-28, Semen arachidis hypogaeae dregs of rice 19-20, sprout brown rice 20-21, papaya powder 8-9, soy bean powder 10-11, imported fish meal 9-10, modified tapioca starch solution 11-12, Viscotrol C 1-2, hedysarum polybotys saccharide 2-3, the former powder 2-3 of humic acid, vitamin complex 0.2-0.4, amino acid/11-2, trace element 0.1-0.2, ionic liquid emulsification propolis 2.5-3.5, monocalcium phosphate 0.2-0.3, polyglutamic acid 1.1-1.2, Trisodium Citrate 0.6-0.7, yeast 2-3, appropriate water.

Adopt propolis as an antibacterial and Oudemansiella Radicata efficient culture medium preparation method that the is dual material of nutrition, comprise the following steps:

(1) by thermal destruction water-soluble poval macroporous microsphere, the mixing of half yeast, and add gross weight 2-3 water doubly, moderate-speed mixer 2-3h, adopt spray-drying process dry thermal destruction water-soluble poval macroporous microsphere absorption load zymophyte for subsequent use; By superstrength porous microsphere active carbon, Porous Chitosan Microspheres, the mixing of ionic liquid emulsification propolis, and add gross weight 3-4 water doubly, at 30-35 DEG C, heated at high speed stirs 1.5-2h, obtains modification propolis for subsequent use;

(2) by peanut pomace, Chinese torreya pomace, rice root, purple perilla oil meal, sesame seed meal, the Semen arachidis hypogaeae dregs of rice, sprout brown rice co-grinding 20-30min, slag must be pulverized for subsequent use;

(3) by Viscotrol C, hedysarum polybotys saccharide, the former powder of humic acid, vitamin complex, amino acid, trace element, monocalcium phosphate, polyglutamic acid, Trisodium Citrate mixing, and add gross weight 2-3 water doubly, at room temperature be stirred to evenly, obtain nutritive medium for subsequent use;

(4) slag, papaya powder, soy bean powder, imported fish meal, modified tapioca starch solution, modification propolis, nutritive medium mixing will be pulverized, and add gross weight 2-3 water mixing and stirring doubly, be divided into two portions, half is placed through in the top fermentation tank of the fermentor tank up and down connected up and down that seal valve connects, and access the yeast of residue one semiactive, anaerobic fermentation 10-11d at 23-24 DEG C, obtains fermented liquid one for subsequent use; Second half puts into the bottom fermentation tank of band whipping appts, and the zymophyte that the thermal destruction water-soluble poval macroporous microsphere absorption of access activation loads, anaerobic fermentation 10-11d at 21-22 DEG C, obtain fermented liquid two for subsequent use, open seal valve, fermented liquid one is flowed in fermented liquid two, and the whipping appts starting bottom fermentation tank is stirred to evenly simultaneously, close seal valve afterwards, in bottom fermentation tank, sealed fermenting 28-30d at 22-23 DEG C, during the fermentation, every 2-3d stirs once, stirs 30-40min at every turn, obtains fermention medium for subsequent use;

(5) fermention medium is poured out fermentor tank, spread airing 5-10d out, adjusting moisture is afterwards the 60-62% of gross weight, adopt the pack of double-layer polyethylene plastics bag, then put into double-layered water bath device, in the water bath of 100 DEG C, 100 DEG C of sterilizing 22-24h, to obtain final product.

Advantage of the present invention is:

The modified latex that the present invention adopts superstrength porous microsphere active carbon, Porous Chitosan Microspheres, prepared by ionic liquid emulsification propolis, join in substratum as nutritive substance and antimicrobial substance, improve the nutrition of substratum and anti-miscellaneous bacteria effect, all have the feature of natural environmental-protective, adopt peanut pomace, Chinese torreya pomace, rice root, purple perilla oil meal, sesame seed meal, the Semen arachidis hypogaeae dregs of rice, sprout brown rice, papaya powder, soy bean powder, imported fish meal, modified tapioca starch solution etc. through fermentation for collybia radicata culture medium, nutritious, in the process made, the zymophyte adopting the absorption of thermal destruction water-soluble poval macroporous microsphere to load is fermented, effectively have adjusted fermenting speed, promote the decomposition of fermentation substrate, make medium nutrient content more abundant and balanced, the absorption of more convenient Oudemansiella Radicata and utilization, for the Efficient Cultivation of Oudemansiella Radicata provides foundation.

Embodiment

A kind of propolis that adopts is as antibacterial and Oudemansiella Radicata efficient culture medium that the is dual material of nutrition, comprise following parts by weight of component: thermal destruction water-soluble poval macroporous microsphere 3, superstrength porous microsphere active carbon 3, Porous Chitosan Microspheres 3, peanut pomace 25, Chinese torreya pomace 16, rice root 21, purple perilla oil meal 24, sesame seed meal 27, the Semen arachidis hypogaeae dregs of rice 19, sprout brown rice 20, papaya powder 8, soy bean powder 10, imported fish meal 9, modified tapioca starch solution 11, Viscotrol C 1, hedysarum polybotys saccharide 2, the former powder 2 of humic acid, vitamin complex 0.2, amino acid/11, trace element 0.1, ionic liquid emulsification propolis 2.5, monocalcium phosphate 0.2, polyglutamic acid 1.1, Trisodium Citrate 0.6, yeast 2, appropriate water.

Adopt propolis as an antibacterial and Oudemansiella Radicata efficient culture medium preparation method that the is dual material of nutrition, comprise the following steps:

(1) by thermal destruction water-soluble poval macroporous microsphere, the mixing of half yeast, and add the water of gross weight 2 times, moderate-speed mixer 2h, adopt spray-drying process dry the zymophyte that loads of thermal destruction water-soluble poval macroporous microsphere absorption is for subsequent use; By superstrength porous microsphere active carbon, Porous Chitosan Microspheres, the mixing of ionic liquid emulsification propolis, and add the water of gross weight 3 times, at 30 DEG C, heated at high speed stirs 1.5h, obtains modification propolis for subsequent use;

(2) by peanut pomace, Chinese torreya pomace, rice root, purple perilla oil meal, sesame seed meal, the Semen arachidis hypogaeae dregs of rice, sprout brown rice co-grinding 20min, slag must be pulverized for subsequent use;

(3) by Viscotrol C, hedysarum polybotys saccharide, the former powder of humic acid, vitamin complex, amino acid, trace element, monocalcium phosphate, polyglutamic acid, Trisodium Citrate mixing, and add the water of gross weight 2 times, be at room temperature stirred to evenly, obtain nutritive medium for subsequent use;

(4) slag, papaya powder, soy bean powder, imported fish meal, modified tapioca starch solution, modification propolis, nutritive medium mixing will be pulverized, and add the water mixing and stirring of gross weight 2 times, be divided into two portions, half is placed through in the top fermentation tank of the fermentor tank up and down connected up and down that seal valve connects, and access the yeast of residue one semiactive, anaerobic fermentation 10d at 23 DEG C, obtains fermented liquid one for subsequent use; Second half puts into the bottom fermentation tank of band whipping appts, and the zymophyte that the thermal destruction water-soluble poval macroporous microsphere absorption of access activation loads, anaerobic fermentation 10d at 21 DEG C, obtain fermented liquid two for subsequent use, open seal valve, fermented liquid one is flowed in fermented liquid two, and the whipping appts starting bottom fermentation tank is stirred to evenly simultaneously, close seal valve afterwards, in bottom fermentation tank, sealed fermenting 28d at 22 DEG C, during the fermentation, every 2d stirs once, stirs 30min at every turn, obtains fermention medium for subsequent use;

(5) fermention medium is poured out fermentor tank, spread airing 5d out, adjusting moisture is afterwards 60% of gross weight, and adopt the pack of double-layer polyethylene plastics bag, then put into double-layered water bath device, in the water bath of 100 DEG C, 100 DEG C of sterilizing 22h, to obtain final product.

Claims (2)

1. one kind adopts propolis as antibacterial and Oudemansiella Radicata efficient culture medium that the is dual material of nutrition, it is characterized in that, comprise following parts by weight of component: thermal destruction water-soluble poval macroporous microsphere 3-4, superstrength porous microsphere active carbon 3-4, Porous Chitosan Microspheres 3-4, peanut pomace 25-26, Chinese torreya pomace 16-17, rice root 21-22, purple perilla oil meal 24-26, sesame seed meal 27-28, Semen arachidis hypogaeae dregs of rice 19-20, sprout brown rice 20-21, papaya powder 8-9, soy bean powder 10-11, imported fish meal 9-10, modified tapioca starch solution 11-12, Viscotrol C 1-2, hedysarum polybotys saccharide 2-3, the former powder 2-3 of humic acid, vitamin complex 0.2-0.4, amino acid/11-2, trace element 0.1-0.2, ionic liquid emulsification propolis 2.5-3.5, monocalcium phosphate 0.2-0.3, polyglutamic acid 1.1-1.2, Trisodium Citrate 0.6-0.7, yeast 2-3, appropriate water.

2. adopt propolis as an antibacterial and Oudemansiella Radicata efficient culture medium preparation method that the is dual material of nutrition, it is characterized in that, comprise the following steps:

(1) by thermal destruction water-soluble poval macroporous microsphere, the mixing of half yeast, and add gross weight 2-3 water doubly, moderate-speed mixer 2-3h, adopt spray-drying process dry thermal destruction water-soluble poval macroporous microsphere absorption load zymophyte for subsequent use; By superstrength porous microsphere active carbon, Porous Chitosan Microspheres, the mixing of ionic liquid emulsification propolis, and add gross weight 3-4 water doubly, at 30-35 DEG C, heated at high speed stirs 1.5-2h, obtains modification propolis for subsequent use;

(2) by peanut pomace, Chinese torreya pomace, rice root, purple perilla oil meal, sesame seed meal, the Semen arachidis hypogaeae dregs of rice, sprout brown rice co-grinding 20-30min, slag must be pulverized for subsequent use;

(3) by Viscotrol C, hedysarum polybotys saccharide, the former powder of humic acid, vitamin complex, amino acid, trace element, monocalcium phosphate, polyglutamic acid, Trisodium Citrate mixing, and add gross weight 2-3 water doubly, at room temperature be stirred to evenly, obtain nutritive medium for subsequent use;

(4) slag, papaya powder, soy bean powder, imported fish meal, modified tapioca starch solution, modification propolis, nutritive medium mixing will be pulverized, and add gross weight 2-3 water mixing and stirring doubly, be divided into two portions, half is placed through in the top fermentation tank of the fermentor tank up and down connected up and down that seal valve connects, and access the yeast of residue one semiactive, anaerobic fermentation 10-11d at 23-24 DEG C, obtains fermented liquid one for subsequent use; Second half puts into the bottom fermentation tank of band whipping appts, and the zymophyte that the thermal destruction water-soluble poval macroporous microsphere absorption of access activation loads, anaerobic fermentation 10-11d at 21-22 DEG C, obtain fermented liquid two for subsequent use, open seal valve, fermented liquid one is flowed in fermented liquid two, and the whipping appts starting bottom fermentation tank is stirred to evenly simultaneously, close seal valve afterwards, in bottom fermentation tank, sealed fermenting 28-30d at 22-23 DEG C, during the fermentation, every 2-3d stirs once, stirs 30-40min at every turn, obtains fermention medium for subsequent use;

(5) fermention medium is poured out fermentor tank, spread airing 5-10d out, adjusting moisture is afterwards the 60-62% of gross weight, adopt the pack of double-layer polyethylene plastics bag, then put into double-layered water bath device, in the water bath of 100 DEG C, 100 DEG C of sterilizing 22-24h, to obtain final product.