CN105130636A - Efficient culture medium for achieving efficient cultivation of armillaria mellea by improving extracellular enzyme activity thereof and preparation method therefor - Google Patents

Abstract

The present invention discloses an efficient culture medium for achieving efficient cultivation of armillaria mellea by improving extracellular enzyme activity thereof. The efficient culture medium comprises the following components in parts by weight: polycaprolactone microspheres, porous bioactive calcium phosphate microspheres, pine needle powder, edible fungus residue, cucurmosin, expanded rice bran husks, yeast cell walls, and yeast. According to the efficient culture medium provided by the invention, the pine needle power is subjected to hydrolysis performed by modified cellulases, and is used in combination with the edible fungus residue and the cucurmosin. The expanded rice bran husks are fermented to prepare the armillaria mellea culture medium, so that reasonable combination and abundant nutrition can improve the extracellular enzyme activity of the armillaria mellea, thereby improving cultivation efficiency of an extracellular enzyme. The used modified cellulases exist in the culture medium for a long time so as to decompose cellulose continuously and collaboratively and provide nutrition for the armillaria mellea. The porous bioactive calcium phosphate microspheres are used to adsorb the loaded yeast for fermentation, so that activity of the yeast is increased and decomposition of the culture medium raw material is promoted, thereby facilitating absorption and application, and providing a foundation for efficient cultivation of the armillaria mellea.

Description

A kind of by improving honey mushroom Extracellular enzyme activity efficient culture medium realizing its Efficient Cultivation and preparation method thereof

Technical field

The present invention relates to culture medium of edible fungus field, particularly relating to a kind of by improving honey mushroom Extracellular enzyme activity efficient culture medium realizing its Efficient Cultivation and preparation method thereof.

Background technology

Honey mushroom has another name called excellent mushroom, and being a kind of facultative parasitism fungi of Agaricales swollen coral Cordycepps Armillaria, is a kind of important edible fungus, is the Major Nutrient source promoting that rhizoma Gastrodiae and umbellate pore furgus grow simultaneously.Honey mushroom can not directly utilize the macromole nutrition in substratum to misunderstand, need to be degraded into small-molecule substance by extracellular enzyme to absorb, CMC enzyme belongs to Mierocrystalline cellulose enzyme decomposition of cellulose, zytase can decompose hemicellulose, honey mushroom Extracellular laccase is the main enzyme that honey mushroom secondary growth metabolic stage produces, and the regulation and control being realized honey mushroom growth by enzyme are keys of honey mushroom Efficient Cultivation; The nutritive ingredient of different culture media formula is comparatively large on enzymic activity impact, and thus the substratum of research and development raising honey mushroom Extracellular enzyme activity is most important.

The micropore of the microballoon of vesicular structure is covered with whole microballoon and gos deep into center by surface, time as carrier, have, adsorption site is many, the features such as large specific surface area and strong adsorptive power, can play and change by features such as object carrier solvability, releasabilities, through being often used as the carrier of slow release fertilizer; In the preparation process of the submerged fermentation efficient culture medium of edible fungus culturing, the decomposition of culture medium raw material and fermentation directly decide the quality of substratum, and then determine that cultivation uses the effect of gold; The control of relevant enzyme and zymophyte activity is key and the difficult point of preparation submerged fermentation efficient culture medium, do not have the report of microballoon as the control of relevant enzyme and zymophyte activity in substratum making processes of vesicular structure at present, but according to the large specific surface area of porous microsphere, be conducive to sticking in a large number of cell, being conducive to the transmission of nutritive substance and the discharge of metabolic substd and this character of intercellular interaction, should be the control that can be used to enzyme and zymophyte activity.

Summary of the invention

The feature of honey mushroom of the present invention and Efficient Cultivation demand, provide a kind of by improving honey mushroom Extracellular enzyme activity efficient culture medium realizing its Efficient Cultivation and preparation method thereof.

The present invention is achieved by the following technical solutions:

A kind of efficient culture medium realizing its Efficient Cultivation by improving honey mushroom Extracellular enzyme activity, comprise following parts by weight of component: polycaprolactone microballoon sphere 2-3, bioactivity calcium phosphate porous microsphere 3-4, become thoroughly decomposed cattle and sheep excrement 10-11, edible fungi residue 16-17, sawdust 14-16, Folium Arachidis hypogaeae powder 15-16, pine needle powder 17-19, Loquat Leaf powder 16-17, Leaf of Balsampear powder 14-15, chick bone powder 6-7, soya bean slag 8-9, Cucurmosin 4-5, Chinese yam protain 3-4, fish slurry albumen 5-6, puffed rice bran shell 16-17, polished rice bud powder 18-10, Herba Andrographis dry powder 2-3, yeast cell wall 1-2, cellulase 2-3, aminoacids complex 1-2, sodium polyaspartate 1.5-2, tripoly phosphate sodium STPP 0.8-1, trimethyl-glycine 1-1.1, yeast 2-3, appropriate water.

Realizing an efficient culture medium preparation method for its Efficient Cultivation by improving honey mushroom Extracellular enzyme activity, comprising the following steps:

(1) by bioactivity calcium phosphate porous microsphere, half yeast, the mixing of yeast cell wall, and add gross weight 2-3 water doubly, moderate-speed mixer 2-3h, the zymophyte obtaining bioactivity calcium phosphate porous microsphere absorption loading is for subsequent use; By polycaprolactone microballoon sphere, cellulase mixing, and add gross weight 3-4 water doubly, middling speed heated and stirred 1-1.2h at 20-23 DEG C, obtains modified-cellulose enzyme for subsequent use;

(2) by Folium Arachidis hypogaeae powder, pine needle powder, Loquat Leaf powder, Leaf of Balsampear powder co-grinding 20-30min, obtain comminuted powder, by comminuted powder, the mixing of modified-cellulose enzyme, and add gross weight 1-2 water doubly, at 38-40 DEG C, heat stirring at low speed 2-3h, obtain thick enzymolysis solution for subsequent use;

(3) by compound amino acid powder, sodium polyaspartate, tripoly phosphate sodium STPP, trimethyl-glycine mixing, and add gross weight 2-3 water doubly, be at room temperature stirred to evenly, obtain nutritive medium for subsequent use;

(4) by thick enzymolysis solution, the cattle and sheep excrement that becomes thoroughly decomposed, edible fungi residue, sawdust, chick bone powder, soya bean slag, Cucurmosin, Chinese yam protain, fish slurry albumen, puffed rice bran shell, polished rice bud powder, Herba Andrographis dry powder, nutritive medium mixing, and add gross weight 2-3 water mixing and stirring doubly, be divided into two portions, half is placed through in the top fermentation tank of the fermentor tank up and down connected up and down that seal valve connects, and access the yeast of residue one semiactive, anaerobic fermentation 12-14d at 23-24 DEG C, obtains fermented liquid one for subsequent use; Second half puts into the bottom fermentation tank of band whipping appts, and the zymophyte that the bioactivity calcium phosphate porous microsphere absorption of access activation loads, anaerobic fermentation 12-14d at 21-22 DEG C, obtain fermented liquid two for subsequent use, open seal valve, fermented liquid one is flowed in fermented liquid two, and the whipping appts starting bottom fermentation tank is stirred to evenly simultaneously, close seal valve afterwards, in bottom fermentation tank, sealed fermenting 22-25d at 22-23 DEG C, during the fermentation, every 2-3d stirs once, stirs 30-40min at every turn, obtains fermention medium for subsequent use;

(5) fermention medium is poured out fermentor tank, spread airing 5-10d out, adjusting moisture is afterwards the 60-62% of gross weight, adopt the pack of double-layer polyethylene plastics bag, then put into double-layered water bath device, in the water bath of 100 DEG C, 100 DEG C of sterilizing 22-24h, to obtain final product.

Advantage of the present invention is:

The present invention adopts Folium Arachidis hypogaeae powder, pine needle powder, Loquat Leaf powder, Leaf of Balsampear powder coordinates ripe cattle and sheep excrement after modified-cellulose enzyme enzymolysis, edible fungi residue, sawdust, chick bone powder, soya bean slag, Cucurmosin, Chinese yam protain, fish slurry albumen, puffed rice bran shell, polished rice bud powder, Herba Andrographis dry powder through fermentation for honey mushroom substratum, rational collocation, abundant nutrition, honey mushroom Extracellular enzyme activity can be improved, and then improve extracellular enzyme cultivation efficiency, the modified-cellulose enzyme adopted, lifetime is long in the medium, collaborative decomposition of cellulose can be continued, nutrition is provided to honey mushroom, the zymophyte adopting the absorption of bioactivity calcium phosphate porous microsphere to load is fermented, effectively raise saccharomycetic activity, facilitate the decomposition of culture medium raw material, make substratum balanced in nutrition, be convenient to absorb and application, for the Efficient Cultivation of honey mushroom provides the foundation.

Embodiment

A kind of efficient culture medium realizing its Efficient Cultivation by improving honey mushroom Extracellular enzyme activity, comprise following parts by weight of component: polycaprolactone microballoon sphere 2, bioactivity calcium phosphate porous microsphere 3, become thoroughly decomposed cattle and sheep excrement 10, edible fungi residue 16, sawdust 14-16, Folium Arachidis hypogaeae powder 15, pine needle powder 17, Loquat Leaf powder 16, Leaf of Balsampear powder 14, chick bone powder 6, soya bean slag 8, Cucurmosin 4, Chinese yam protain 3, fish slurry albumen 5, puffed rice bran shell 16, polished rice bud powder 18, Herba Andrographis dry powder 2, yeast cell wall 1, cellulase 2, aminoacids complex 1, sodium polyaspartate 1.5, tripoly phosphate sodium STPP 0.8, trimethyl-glycine 1, yeast 2, appropriate water.

Realizing an efficient culture medium preparation method for its Efficient Cultivation by improving honey mushroom Extracellular enzyme activity, comprising the following steps:

(1) by bioactivity calcium phosphate porous microsphere, half yeast, the mixing of yeast cell wall, and add the water of gross weight 2 times, moderate-speed mixer 2h, the zymophyte obtaining bioactivity calcium phosphate porous microsphere absorption loading is for subsequent use; By polycaprolactone microballoon sphere, cellulase mixing, and add the water of gross weight 3 times, middling speed heated and stirred 1h at 20 DEG C, obtains modified-cellulose enzyme for subsequent use;

(2) by Folium Arachidis hypogaeae powder, pine needle powder, Loquat Leaf powder, Leaf of Balsampear powder co-grinding 20min, obtain comminuted powder, by comminuted powder, the mixing of modified-cellulose enzyme, and add the water of gross weight 1 times, at 38 DEG C, heat stirring at low speed 2h, obtain thick enzymolysis solution for subsequent use;

(3) by compound amino acid powder, sodium polyaspartate, tripoly phosphate sodium STPP, trimethyl-glycine mixing, and add the water of gross weight 2 times, be at room temperature stirred to evenly, obtain nutritive medium for subsequent use;

(4) by thick enzymolysis solution, the cattle and sheep excrement that becomes thoroughly decomposed, edible fungi residue, sawdust, chick bone powder, soya bean slag, Cucurmosin, Chinese yam protain, fish slurry albumen, puffed rice bran shell, polished rice bud powder, Herba Andrographis dry powder, nutritive medium mixing, and add the water mixing and stirring of gross weight 2 times, be divided into two portions, half is placed through in the top fermentation tank of the fermentor tank up and down connected up and down that seal valve connects, and access the yeast of residue one semiactive, anaerobic fermentation 12d at 23 DEG C, obtains fermented liquid one for subsequent use; Second half puts into the bottom fermentation tank of band whipping appts, and the zymophyte that the bioactivity calcium phosphate porous microsphere absorption of access activation loads, anaerobic fermentation 12d at 21 DEG C, obtain fermented liquid two for subsequent use, open seal valve, fermented liquid one is flowed in fermented liquid two, and the whipping appts starting bottom fermentation tank is stirred to evenly simultaneously, close seal valve afterwards, in bottom fermentation tank, sealed fermenting 22d at 22 DEG C, during the fermentation, every 2d stirs once, stirs 30min at every turn, obtains fermention medium for subsequent use;

(5) fermention medium is poured out fermentor tank, spread airing 5d out, adjusting moisture is afterwards 60% of gross weight, and adopt the pack of double-layer polyethylene plastics bag, then put into double-layered water bath device, in the water bath of 100 DEG C, 100 DEG C of sterilizing 22h, to obtain final product.

Claims (2)

1. one kind realizes the efficient culture medium of its Efficient Cultivation by improving honey mushroom Extracellular enzyme activity, it is characterized in that, comprise following parts by weight of component: polycaprolactone microballoon sphere 2-3, bioactivity calcium phosphate porous microsphere 3-4, become thoroughly decomposed cattle and sheep excrement 10-11, edible fungi residue 16-17, sawdust 14-16, Folium Arachidis hypogaeae powder 15-16, pine needle powder 17-19, Loquat Leaf powder 16-17, Leaf of Balsampear powder 14-15, chick bone powder 6-7, soya bean slag 8-9, Cucurmosin 4-5, Chinese yam protain 3-4, fish slurry albumen 5-6, puffed rice bran shell 16-17, polished rice bud powder 18-10, Herba Andrographis dry powder 2-3, yeast cell wall 1-2, cellulase 2-3, aminoacids complex 1-2, sodium polyaspartate 1.5-2, tripoly phosphate sodium STPP 0.8-1, trimethyl-glycine 1-1.1, yeast 2-3, appropriate water.

2. realizing an efficient culture medium preparation method for its Efficient Cultivation by improving honey mushroom Extracellular enzyme activity, it is characterized in that, comprise the following steps:

(1) by bioactivity calcium phosphate porous microsphere, half yeast, the mixing of yeast cell wall, and add gross weight 2-3 water doubly, moderate-speed mixer 2-3h, the zymophyte obtaining bioactivity calcium phosphate porous microsphere absorption loading is for subsequent use; By polycaprolactone microballoon sphere, cellulase mixing, and add gross weight 3-4 water doubly, middling speed heated and stirred 1-1.2h at 20-23 DEG C, obtains modified-cellulose enzyme for subsequent use;

(2) by Folium Arachidis hypogaeae powder, pine needle powder, Loquat Leaf powder, Leaf of Balsampear powder co-grinding 20-30min, obtain comminuted powder, by comminuted powder, the mixing of modified-cellulose enzyme, and add gross weight 1-2 water doubly, at 38-40 DEG C, heat stirring at low speed 2-3h, obtain thick enzymolysis solution for subsequent use;

(3) by compound amino acid powder, sodium polyaspartate, tripoly phosphate sodium STPP, trimethyl-glycine mixing, and add gross weight 2-3 water doubly, be at room temperature stirred to evenly, obtain nutritive medium for subsequent use;

(4) by thick enzymolysis solution, the cattle and sheep excrement that becomes thoroughly decomposed, edible fungi residue, sawdust, chick bone powder, soya bean slag, Cucurmosin, Chinese yam protain, fish slurry albumen, puffed rice bran shell, polished rice bud powder, Herba Andrographis dry powder, nutritive medium mixing, and add gross weight 2-3 water mixing and stirring doubly, be divided into two portions, half is placed through in the top fermentation tank of the fermentor tank up and down connected up and down that seal valve connects, and access the yeast of residue one semiactive, anaerobic fermentation 12-14d at 23-24 DEG C, obtains fermented liquid one for subsequent use; Second half puts into the bottom fermentation tank of band whipping appts, and the zymophyte that the bioactivity calcium phosphate porous microsphere absorption of access activation loads, anaerobic fermentation 12-14d at 21-22 DEG C, obtain fermented liquid two for subsequent use, open seal valve, fermented liquid one is flowed in fermented liquid two, and the whipping appts starting bottom fermentation tank is stirred to evenly simultaneously, close seal valve afterwards, in bottom fermentation tank, sealed fermenting 22-25d at 22-23 DEG C, during the fermentation, every 2-3d stirs once, stirs 30-40min at every turn, obtains fermention medium for subsequent use;

(5) fermention medium is poured out fermentor tank, spread airing 5-10d out, adjusting moisture is afterwards the 60-62% of gross weight, adopt the pack of double-layer polyethylene plastics bag, then put into double-layered water bath device, in the water bath of 100 DEG C, 100 DEG C of sterilizing 22-24h, to obtain final product.