CN105130633A - Photo-stimulated pholiota nameko culture medium and preparation method therefor - Google Patents

Abstract

The present invention discloses a photo-stimulated pholiota nameko culture medium. The photo-stimulated pholiota nameko culture medium comprises the following components in parts by weight: porous microspheroidal talc powder, porous chitosan microspheres for loading nano degradation agents for light absorbing and heating, high protein corn starch, polyrhachis vicina roger, chips of broad-leaf tree, cucumber-seed powder, sawdust, citric acid waste, honeycomb, glycogen, saccharomycetes 2-3, peptone, organic potassium phosphate, magnesium amino acid and the like. According to the present invention, the pholiota nameko culture medium can be prepared through the fermentation of high protein corn starch and polyrhachis vicina roger, and the nutrients are rich; and fermentation bacteria adsorbed by the porous chitosan microspheres for loading nano degradation agents for light absorbing and heating is used for fermentation, the activity of release of the fermentation bacteria is improved during the fermentation process along with light irradiation, the degree of decomposition of the substrate is improved, rich and balanced nutritive substances are provided for pholiota nameko, and cultivation with high efficiency and high quality of pholiota nameko can be realized.

Description

A kind of light urgees HUAZIGU substratum and preparation method thereof

Technical field

The present invention relates to culture medium of edible fungus field, particularly relate to a kind of light and urge HUAZIGU substratum and preparation method thereof.

Background technology

HUAZIGU contains crude protein, fat, carbohydrate, robust fibre, ash content, calcium, phosphorus, iron, vitamins B, vitamins C, nicotinic acid and human body other each seed amino acid necessary, and the viscous substance being attached to sliding mushroom cap surface is a kind of nucleic acid, to keeping the energy of human body and mentally benefiting, and also has the effect of Tumor suppression.Traditional cultivation fertilizer is with corn cob, wood chip for major ingredient, then to be equipped with cotton seed hulls, wheat bran, Semen Maydis powder, lime and some additives be auxiliary material.But these raw material resources are fewer and feweri, affect output and the healthy nutritive value of HUAZIGU, find new starting material to replace or supplement existing raw material resources or by illumination, realize HUAZIGU high-effect high-quality cultivation, become extremely urgent thing.

The micropore of the microballoon of vesicular structure is covered with whole microballoon and gos deep into center by surface, time as carrier, have, adsorption site is many, the features such as large specific surface area and strong adsorptive power, can play and change by features such as object carrier solvability, releasabilities, through being often used as the carrier of slow release fertilizer; In the preparation process of the submerged fermentation efficient culture medium of edible fungus culturing, the decomposition of culture medium raw material and fermentation directly decide the quality of substratum, and then determine that cultivation uses the effect of gold; The control of relevant enzyme and zymophyte activity is key and the difficult point of preparation submerged fermentation efficient culture medium, do not have the report of microballoon as the control of relevant enzyme and zymophyte activity in substratum making processes of vesicular structure at present, but according to the large specific surface area of porous microsphere, be conducive to sticking in a large number of cell, being conducive to the transmission of nutritive substance and the discharge of metabolic substd and this character of intercellular interaction, should be the control that can be used to enzyme and zymophyte activity.

Summary of the invention

Order of the present invention, for the Efficient Cultivation demand of HUAZIGU, provides a kind of light to urge HUAZIGU substratum and preparation method thereof.

The present invention is achieved by the following technical solutions:

A kind of light urgees HUAZIGU substratum, comprise following parts by weight of component: porous microsphere shape talcum powder 2.5-3.5, load the Porous Chitosan Microspheres 3-4 of nanometer extinction intensification degradation agents, high protein cereal starch 20-30, black multi-thorn ant powder 19-20, oyster digested tankage 17-18, locust powder 15-16, hardwood sawdust 30-33, wheat straw grass 23-24, potato vine 19-20, sunflower straw 18-19, cucumber seed powder 7-8, wood chaff 18-20, citric acid waste 8-9, honeycomb element 0.9-1, sucrose 2-3, calcium malate 1.3-1.5, amino acid/11 .1-1.2, glycogen 0.9-1, proteolytic enzyme 1.9-2.1, yeast 2-3, peptone 1.9-2.1, organic phosphoric acid potassium 0.7-0.8, amino acid-magnesium chelate 0.5-0.6, appropriate water.

A kind of light urgees HUAZIGU medium preparation method, comprises the following steps:

(1) by the Porous Chitosan Microspheres of loading nanometer extinction intensification degradation agents, half yeast, peptone mixing, and add gross weight 2-3 water doubly, moderate-speed mixer 2-3h, the zymophyte that must load the Porous Chitosan Microspheres absorption of nanometer extinction intensification degradation agents is for subsequent use; After porous microsphere shape talcum powder high temperature 150 DEG C calcining 1-1.5h, cooling, and proteolytic enzyme mix and blend 0.5-1h, obtain highly active protein enzyme for subsequent use;

(2) by high protein cereal starch, black multi-thorn ant powder, oyster digested tankage, locust powder, the mixing of highly active protein enzyme, and add gross weight 2-3 water mixing doubly, at 35-40 DEG C, stir enzymolysis 2-3h, obtain thick enzymolysis solution for subsequent use; Wheat straw grass, potato vine, sunflower straw are first put into pulverizer and pulverized 30-40min, puts into bulking machine high temperature puffing process 20-30min afterwards, obtain bulking flour flour for subsequent use;

(3) by honeycomb element, sucrose, calcium malate, amino acid, glycogen, organic phosphoric acid potassium, amino acid-magnesium chelate mixing, and add gross weight 2-3 water doubly, be at room temperature stirred to evenly, obtain nutritive medium for subsequent use;

(4) by thick enzymolysis solution, hardwood sawdust, cucumber seed powder, wooden chaff, citric acid waste, nutritive medium mixing, and add gross weight 2-3 water mixing and stirring doubly, be divided into two portions, half is placed through in the top fermentation tank of the fermentor tank up and down connected up and down that seal valve connects, and access the yeast of residue one semiactive, at 22-24 DEG C, anaerobic printing opacity fermentation 13-14d, obtains fermented liquid one for subsequent use, second half puts into the bottom fermentation tank of band whipping appts, and the zymophyte of the Porous Chitosan Microspheres absorption of the loading nanometer extinction intensification degradation agents of access activation, anaerobic printing opacity fermentation 12-14d at 19-20 DEG C, obtain fermented liquid two for subsequent use, open seal valve, fermented liquid one is flowed in fermented liquid two, and the whipping appts starting bottom fermentation tank is stirred to evenly simultaneously, close seal valve afterwards, in bottom fermentation tank, airtight printing opacity fermentation 20-25d at 20.5-21.5 DEG C, during the fermentation, every 2-3d stirs once, each stirring 35-45min, obtain fermention medium for subsequent use,

(5) fermention medium is poured out fermentor tank, spread airing 6-10d out, adjusting moisture is afterwards the 60-68% of gross weight, adopt the pack of double-layer polyethylene plastics bag, then put into double-layered water bath device, in the water bath of 100 DEG C, 100 DEG C of sterilizing 22-28h, to obtain final product.

Advantage of the present invention is:

High protein cereal starch of the present invention, black multi-thorn ant powder, oyster digested tankage, locust powder, hardwood sawdust, wheat straw grass, potato vine, sunflower straw, cucumber seed powder, wooden chaff, citric acid waste, honeycomb element etc. through fermentation for HUAZIGU substratum, nutritious; The zymophyte of the Porous Chitosan Microspheres absorption loading nanometer extinction intensification degradation agents is adopted to ferment, during the fermentation along with illumination, yeast release is active to improve, improve the degree of decomposition of substrate, for HUAZIGU provides abundant balanced nutritive substance, the high-effect high-quality cultivation of HUAZIGU can be realized.

Embodiment

A kind of light urgees HUAZIGU substratum, comprise following parts by weight of component: porous microsphere shape talcum powder 2.5, load the Porous Chitosan Microspheres 3 of nanometer extinction intensification degradation agents, high protein cereal starch 20, black multi-thorn ant powder 19, oyster digested tankage 17, locust powder 15, hardwood sawdust 30, wheat straw grass 23, potato vine 19, sunflower straw 18, cucumber seed powder 7, wood chaff 18, citric acid waste 8, honeycomb element 0.9, sucrose 2, calcium malate 1.3, amino acid/11 .1, glycogen 0.9, proteolytic enzyme 1.9, yeast 2, peptone 1.9, organic phosphoric acid potassium 0.7, amino acid-magnesium chelate 0.5, appropriate water.

A kind of light urgees HUAZIGU medium preparation method, comprises the following steps:

(1) by the Porous Chitosan Microspheres of loading nanometer extinction intensification degradation agents, half yeast, peptone mixing, and add the water of gross weight 2 times, moderate-speed mixer 2h, the zymophyte that must load the Porous Chitosan Microspheres absorption of nanometer extinction intensification degradation agents is for subsequent use; After porous microsphere shape talcum powder high temperature 150 DEG C calcining 1h, cooling, and proteolytic enzyme mix and blend 0.5h, obtain highly active protein enzyme for subsequent use;

(2) by high protein cereal starch, black multi-thorn ant powder, oyster digested tankage, locust powder, the mixing of highly active protein enzyme, and add the water mixing of gross weight 2 times, at 35 DEG C, stir enzymolysis 2h, obtain thick enzymolysis solution for subsequent use; Wheat straw grass, potato vine, sunflower straw are first put into pulverizer and pulverized 30min, puts into bulking machine high temperature puffing process 20min afterwards, obtain bulking flour flour for subsequent use;

(3) by honeycomb element, sucrose, calcium malate, amino acid, glycogen, organic phosphoric acid potassium, amino acid-magnesium chelate mixing, and add the water of gross weight 2 times, be at room temperature stirred to evenly, obtain nutritive medium for subsequent use;

(4) by thick enzymolysis solution, hardwood sawdust, cucumber seed powder, wooden chaff, citric acid waste, nutritive medium mixing, and add the water mixing and stirring of gross weight 2 times, be divided into two portions, half is placed through in the top fermentation tank of the fermentor tank up and down connected up and down that seal valve connects, and access the yeast of residue one semiactive, at 22 DEG C, anaerobic printing opacity fermentation 13d, obtains fermented liquid one for subsequent use; Second half puts into the bottom fermentation tank of band whipping appts, and the zymophyte of the Porous Chitosan Microspheres absorption of the loading nanometer extinction intensification degradation agents of access activation, anaerobic printing opacity fermentation 12d at 19 DEG C, obtain fermented liquid two for subsequent use, open seal valve, fermented liquid one is flowed in fermented liquid two, and the whipping appts starting bottom fermentation tank is stirred to evenly simultaneously, close seal valve afterwards, in bottom fermentation tank, airtight printing opacity fermentation 20d at 20.5 DEG C, during the fermentation, every 2d stirs once, stirs 35min at every turn, obtains fermention medium for subsequent use;

(5) fermention medium is poured out fermentor tank, spread airing 6d out, adjusting moisture is afterwards 60% of gross weight, and adopt the pack of double-layer polyethylene plastics bag, then put into double-layered water bath device, in the water bath of 100 DEG C, 100 DEG C of sterilizing 22h, to obtain final product.

Claims (2)

1. a light urgees HUAZIGU substratum, it is characterized in that, comprise following parts by weight of component: porous microsphere shape talcum powder 2.5-3.5, load the Porous Chitosan Microspheres 3-4 of nanometer extinction intensification degradation agents, high protein cereal starch 20-30, black multi-thorn ant powder 19-20, oyster digested tankage 17-18, locust powder 15-16, hardwood sawdust 30-33, wheat straw grass 23-24, potato vine 19-20, sunflower straw 18-19, cucumber seed powder 7-8, wood chaff 18-20, citric acid waste 8-9, honeycomb element 0.9-1, sucrose 2-3, calcium malate 1.3-1.5, amino acid/11 .1-1.2, glycogen 0.9-1, proteolytic enzyme 1.9-2.1, yeast 2-3, peptone 1.9-2.1, organic phosphoric acid potassium 0.7-0.8, amino acid-magnesium chelate 0.5-0.6, appropriate water.

2. light urgees a HUAZIGU medium preparation method, it is characterized in that, comprises the following steps:

(1) by the Porous Chitosan Microspheres of loading nanometer extinction intensification degradation agents, half yeast, peptone mixing, and add gross weight 2-3 water doubly, moderate-speed mixer 2-3h, the zymophyte that must load the Porous Chitosan Microspheres absorption of nanometer extinction intensification degradation agents is for subsequent use; After porous microsphere shape talcum powder high temperature 150 DEG C calcining 1-1.5h, cooling, and proteolytic enzyme mix and blend 0.5-1h, obtain highly active protein enzyme for subsequent use;

(2) by high protein cereal starch, black multi-thorn ant powder, oyster digested tankage, locust powder, the mixing of highly active protein enzyme, and add gross weight 2-3 water mixing doubly, at 35-40 DEG C, stir enzymolysis 2-3h, obtain thick enzymolysis solution for subsequent use; Wheat straw grass, potato vine, sunflower straw are first put into pulverizer and pulverized 30-40min, puts into bulking machine high temperature puffing process 20-30min afterwards, obtain bulking flour flour for subsequent use;

(3) by honeycomb element, sucrose, calcium malate, amino acid, glycogen, organic phosphoric acid potassium, amino acid-magnesium chelate mixing, and add gross weight 2-3 water doubly, be at room temperature stirred to evenly, obtain nutritive medium for subsequent use;

(4) by thick enzymolysis solution, hardwood sawdust, cucumber seed powder, wooden chaff, citric acid waste, nutritive medium mixing, and add gross weight 2-3 water mixing and stirring doubly, be divided into two portions, half is placed through in the top fermentation tank of the fermentor tank up and down connected up and down that seal valve connects, and access the yeast of residue one semiactive, at 22-24 DEG C, anaerobic printing opacity fermentation 13-14d, obtains fermented liquid one for subsequent use, second half puts into the bottom fermentation tank of band whipping appts, and the zymophyte of the Porous Chitosan Microspheres absorption of the loading nanometer extinction intensification degradation agents of access activation, anaerobic printing opacity fermentation 12-14d at 19-20 DEG C, obtain fermented liquid two for subsequent use, open seal valve, fermented liquid one is flowed in fermented liquid two, and the whipping appts starting bottom fermentation tank is stirred to evenly simultaneously, close seal valve afterwards, in bottom fermentation tank, airtight printing opacity fermentation 20-25d at 20.5-21.5 DEG C, during the fermentation, every 2-3d stirs once, each stirring 35-45min, obtain fermention medium for subsequent use,

(5) fermention medium is poured out fermentor tank, spread airing 6-10d out, adjusting moisture is afterwards the 60-68% of gross weight, adopt the pack of double-layer polyethylene plastics bag, then put into double-layered water bath device, in the water bath of 100 DEG C, 100 DEG C of sterilizing 22-28h, to obtain final product.