CN103030457A - Culture medium formula and cultivation method for brown hypsizigus marmoreus - Google Patents

Culture medium formula and cultivation method for brown hypsizigus marmoreus Download PDF

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Publication number
CN103030457A
CN103030457A CN2012102742810A CN201210274281A CN103030457A CN 103030457 A CN103030457 A CN 103030457A CN 2012102742810 A CN2012102742810 A CN 2012102742810A CN 201210274281 A CN201210274281 A CN 201210274281A CN 103030457 A CN103030457 A CN 103030457A
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bottle
culture medium
brown
cultivate
crab
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CN2012102742810A
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Chinese (zh)
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王永东
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QINGDAO FENGKE BIOTECHNOLOGY CO Ltd
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QINGDAO FENGKE BIOTECHNOLOGY CO Ltd
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Abstract

The invention discloses a culture medium formula for brown hypsizigus marmoreus. The culture medium formula comprises the following raw materials in percentage by weight: 30 to 40 percent of sawdust, 15 to 25 percent of corncob, 15 to 25 percent of soybean hulls, 5 to 10 percent of rice bran, 5 to 10 percent of wheat bran and 5 to 15 percent of cotton seed hulls. The invention also discloses a method for cultivating the hypsizigus marmoreus by using the culture medium formula.

Description

A kind of brown crab-flavor mushroom culture medium prescription and cultivating method
Technical field
The present invention relates to the edible fungus industrial cultivation technology, or rather, relate to a kind of brown crab-flavor mushroom culture medium prescription and cultivating method.
Background technology
Brown crab-flavor mushroom formal name used at school Hypsizygus marmoreus ( Hypsizygus marmoreus), the Amino Acids in Proteins A wide selection of colours and designs of brown crab-flavor mushroom comprises 8 kinds of essential amino acids, wherein Methionin, arginine content are higher than general mushroom class.And Hypsizygus marmoreus is a kind of protective foods low in calories, low-fat, and its delicious, flat, the Gan Wen of property have the diuresis excreting dampness, the function that invigorating the spleen is quenched the thirst, the effect of the flat liver of heat-clearing.Excellent taste, normal food can improve immunizing power, pre-anti-aging, prolongs life.
On April 4th, 2007 disclosed Chinese patent application CN200510030226.7 number a kind of cultivating method of brown crab-flavor mushroom was disclosed, it is characterized by: be that the Semen Maydis powder of wheat bran, 3-8% of rice bran, the 10-15% of corn cob meal, the 25-30% of wood chip, the 30-40% of 20-30% is as cultivating raw material with weight percent, mix and deliver to bottling machine with transport tape and bottle afterwards, then carry out gland and sterilization, at least vacuumize 2 times, passing into steam heats to 118-121 ℃, be incubated again, then bottle be cooled to 20-22 ℃; Inoculate with inoculation device again, then deliver to culturing room with transport tape and cultivate, incubation time 80-85 days, carry out again the mycelium stimulation processing, again the culturing bottle behind the mycelium stimulation is placed on the bedstead of cultivating chamber, began to gather through 20-22 days.There is following defective in this cultivating method: the cost of (1) substratum is higher; The defectives such as the per unit area yield that (2) uses this culture medium prescription to cultivate is lower.
Summary of the invention
The technical problem that the present invention solves provides a kind of brown crab-flavor mushroom culture medium prescription and cultivating method that cost is low and per unit area yield is high of cultivating.
The present invention is achieved by the following technical solutions: a kind of brown crab-flavor mushroom culture medium prescription comprises wood chip 30%~40%, corn cob 15~25%, soybean hulls 15~25%, rice bran 5~10%, wheat bran 5~10%, cotton seed hulls 5~15%.
Above-mentioned each component is fully mixed rear moisturizing, make and cultivate that water content is 64~66% in the raw material.
A kind of cultivating method of brown crab-flavor mushroom comprises the steps:
(1) raw material mixes: moisturizing after wood chip 30%~40%, corn cob 15~25%, soybean hulls 15~25%, rice bran 5~10%, wheat bran 5~10%, cotton seed hulls 5~15% are fully mixed, and make and cultivate that water content is 64~66% in the raw material, regulating pH is 6~7.
(2) bottling: use the full-automatic streamline of bottling to bottle, require bottling evenly, the degree of packing is moderate, uses the culture bottle of 850cc, and single bottle of weight control restrains 600~640, the basic tight of shoulder, and punching is to the bottle end.
(3) gland: the bottle cap automatic transport is buckled on the bottle of charged to the gland position.
(4) sterilization: a bottle frame subject to sterilization is pushed in the high-pressure sterilizing pot, close to the doorstep, start the operation button, Autoclave is set as vacuum outgas 3 times, sterilizes 90 minutes for 121 ℃.
(5) cooling: sterilization is pushed into sterilizing car in the cooling room after finishing, and the cooling room Temperature Setting is at 20~22 ℃.
(6) inoculation: the bottle that is cooled to below 22 ℃ is inoculated by automatic vaccination machine, and inoculation device is placed in the transfer room of abundant cleaning, and every bottle of original seed connects 30~32 bottles of cultivars, the inoculation after by tape transport to culturing room.
(7) cultivate: culturing room's Temperature Setting is 22~23 ℃, humidity 75~80%, and between gas concentration lwevel 2500~3500PPM, incubation time is 80~82 days.
(8) mycelium stimulation: after cultivating maturation, carry out the moisture that forfeiture in the cultivation was processed and replenished to mycelium stimulation.
(9) urge flower bud, fertility: behind the crab mushroom mycelium stimulation, neatly be placed in rapidly on the cultivation bedstead, 14~15 ℃ of cultivating chamber temperature, humidity 95~98%, gas concentration lwevel is controlled at below the 1500PPM, and 20~21 days begin to gather, about every bottle of output 180~200g.
The culture medium prescription Raw source that the present invention adopts is abundant, cheap, and the cultivation cost is lower, and when using above-mentioned prescription to carry out the cultivation of brown crab-flavor mushroom, product production is stable, every bottle of per unit area yield of 850cc culture bottle 180~200 grams, and per unit area yield is higher.
Embodiment
For technique means, creation characteristic that the present invention is realized, reach purpose and effect is easy to understand, specially further set forth the present invention.
A kind of brown crab-flavor mushroom culture medium prescription of the present invention comprises wood chip 30%~40%(weight percent, lower with), corn cob 15~25%, soybean hulls 15~25%, rice bran 5~10%, wheat bran 5~10%, cotton seed hulls 5~15%.Said components is fully mixed rear moisturizing, make and cultivate that water content is 64~66% in the raw material.
Further specify below in conjunction with concrete cultivating method, the method for using brown crab-flavor mushroom culture medium prescription of the present invention to cultivate is achieved in that
(1) raw material mixes: moisturizing after wood chip 30%~40%, corn cob 15~25%, soybean hulls 15~25%, rice bran 5~10%, wheat bran 5~10%, cotton seed hulls 5~15% are fully mixed, make and cultivate that water content is 64~66% in the raw material, regulating pH with unslaked lime is 6~7, continues to be stirred to bottling and finishes.
(2) bottling: use fully automatic bottle filling machine, require bottling evenly, the degree of packing is moderate, uses every bottle of weight of 850cc Plastic Bottle at 600~640 grams, the basic tight of shoulder, and punching is to the bottle end.
(3) gland: the bottle cap automatic transport is buckled on the bottle of charged to the gland position.
(4) sterilization: adopt high-pressure sterilizing pot, a bottle frame subject to sterilization neatly is placed on the sterilizing car, pushes in the Autoclave, closes to the doorstep, starts the operation button, and Autoclave is set as vacuum outgas 3 times, sterilizes 90 minutes for 121 ℃.
(5) cooling: after sterilization finishes, open the door of tossing about of Autoclave, sterilizing car is pushed in the cooling room, and the cooling room Temperature Setting is at 20~22 ℃.
(6) inoculation: the bottle that is cooled to below 22 ℃ is inoculated by automatic vaccination machine, and inoculation device is placed in the transfer room of abundant cleaning, and every bottle of original seed connects 30~32 bottles of cultivars, the inoculation after by tape transport to culturing room.
(7) cultivate: culturing room's Temperature Setting is 22~23 ℃, humidity 75~80%, and between gas concentration lwevel 2500~3500PPM, incubation time is 80~82 days.
(8) mycelium stimulation: after cultivating maturation, carry out the moisture that forfeiture in the cultivation was processed and replenished to mycelium stimulation.
(9) urge the flower bud fertility: behind the crab mushroom mycelium stimulation, neatly be placed in rapidly on the cultivation bedstead, 14~15 ℃ of cultivating chamber temperature, humidity 95~98%, gas concentration lwevel is controlled at below the 1500PPM, and 20~21 days begin to gather, about every bottle of output 180~200g.
(10) packing of gathering: reach the product of harvesting standard, be pushed into the district that gathers with adopting the mushroom car from cultivation house, put into frame after using air gun to gather, require when gathering to handle with care, reduce the mushroom lid and drop.Cut off the culture material of root, be placed in the pallet, every box nt wt net weight 150 restrains, and after packing with the one-way ventilating film, the carton of packing into (or bubble chamber) is in time put in storage, and temperature of ice house is 3~5 ℃.
(11) dig bottle: the bottle frame after gathering in time is transported to and digs the bottle district, and culture material is excavated, and the empty bottle frame is transported to the bottling district and reuses, and waste material is transported to outside the factory, for the production of fertilizer.
The present invention adopts the culture medium prescription Raw source of stating component abundant, cheap, the cultivation cost is lower, and when using above-mentioned prescription to carry out the cultivation of brown crab-flavor mushroom, product production is stable, every bottle of per unit area yield of 850cc culture bottle 180~200 grams, per unit area yield is higher.
More than describing is embodiments of the invention only, forgives and can understand, and under the prerequisite that does not depart from the present invention's design, all should be included within the technical conceive of the present invention simple modification of the present invention and replacement.

Claims (3)

1. a brown crab-flavor mushroom culture medium prescription is characterized in that: comprise wood chip 30%~40%, corn cob 15~25%, soybean hulls 15~25%, rice bran 5~10%, wheat bran 5~10%, cotton seed hulls 5~15%.
2. brown crab-flavor mushroom culture medium prescription as claimed in claim 1 is characterized in that: above-mentioned each component is fully mixed rear moisturizing, make and cultivate that water content is 64~66% in the raw material.
3. the cultivating method of a brown crab-flavor mushroom is characterized in that comprising the steps:
(1) raw material mixes: moisturizing after wood chip 30%~40%, corn cob 15~25%, soybean hulls 15~25%, rice bran 5~10%, wheat bran 5~10%, cotton seed hulls 5~15% are fully mixed, and make and cultivate that water content is 64~66% in the raw material, regulating pH is 6~7;
(2) bottling: use the full-automatic streamline of bottling to bottle, require bottling evenly, the degree of packing is moderate, uses the culture bottle of 850cc, and single bottle of weight control restrains 600~640, the basic tight of shoulder, and punching is to the bottle end;
(3) gland: the bottle cap automatic transport is buckled on the bottle of charged to the gland position;
(4) sterilization: a bottle frame subject to sterilization is pushed in the high-pressure sterilizing pot, close to the doorstep, start the operation button, Autoclave is set as vacuum outgas 3 times, sterilizes 90 minutes for 121 ℃;
(5) cooling: sterilization is pushed into sterilizing car in the cooling room after finishing, and the cooling room Temperature Setting is at 20~22 ℃;
(6) inoculation: the bottle that is cooled to below 22 ℃ is inoculated by automatic vaccination machine, and inoculation device is placed in the transfer room of abundant cleaning, and every bottle of original seed connects 30~32 bottles of cultivars, the inoculation after by tape transport to culturing room;
(7) cultivate: culturing room's Temperature Setting is 22~23 ℃, humidity 75~80%, and between gas concentration lwevel 2500~3500PPM, incubation time is 80~82 days;
(8) mycelium stimulation: after cultivating maturation, carry out the moisture that forfeiture in the cultivation was processed and replenished to mycelium stimulation;
(9) urge flower bud, fertility: behind the crab mushroom mycelium stimulation, neatly be placed in rapidly on the cultivation bedstead, 14~15 ℃ of cultivating chamber temperature, humidity 95~98%, gas concentration lwevel is controlled at below the 1500PPM, and 20~21 days begin to gather, about every bottle of output 180~200g.
CN2012102742810A 2012-08-03 2012-08-03 Culture medium formula and cultivation method for brown hypsizigus marmoreus Pending CN103030457A (en)

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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104016764A (en) * 2014-05-07 2014-09-03 合肥福泉现代农业科技有限公司 Hypsizigus marmoreus culture medium prepared from cotton straw and preparation method thereof
CN104016761A (en) * 2014-05-07 2014-09-03 合肥福泉现代农业科技有限公司 Hypsizigus marmoreus culture medium and preparation method thereof
CN104016763A (en) * 2014-05-07 2014-09-03 合肥福泉现代农业科技有限公司 Hypsizygus marmoreus culture medium taking peach wood chips as raw materials and preparation method thereof
CN104016765A (en) * 2014-05-07 2014-09-03 合肥福泉现代农业科技有限公司 Hypsizigus marmoreus culture medium with saw dust as raw material and preparation method of hypsizigus marmoreus culture medium
CN104016777A (en) * 2014-05-07 2014-09-03 合肥福泉现代农业科技有限公司 Shiitake culture medium prepared from cottonseed hull and preparation method thereof
CN104782386A (en) * 2015-03-24 2015-07-22 邬金飞 Hypsizygus marmoreus cultivation method
CN104945051A (en) * 2015-06-19 2015-09-30 桂林健成生物科技开发有限公司 Application of seed coats/embryonic bodies and rhizomes obtained after harvest of sprout seedling vegetable to cultivation of hypsizigus marmoreus
CN105474986A (en) * 2015-11-25 2016-04-13 苏州市经纬农产品有限公司 Method for culturing hypsizygus tessellatus
CN107624504A (en) * 2017-11-07 2018-01-26 福建万辰生物科技股份有限公司 A kind of crab flavour mushroom cultural method
CN108251315A (en) * 2018-03-12 2018-07-06 林勇全 Crab flavour mushroom and its cultural method
CN110313346A (en) * 2019-08-05 2019-10-11 江苏华绿生物科技股份有限公司 A kind of corn meal formulation suitable for the plantation of true pleurotus cornucopiae
CN110583368A (en) * 2019-10-10 2019-12-20 西充星河生物科技有限公司 Hypsizigus marmoreus culture medium and application method thereof
CN112293154A (en) * 2020-11-23 2021-02-02 西充星河生物科技有限公司 High-yield cultivation method for white beech mushrooms

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CN1939115A (en) * 2005-09-29 2007-04-04 上海丰科生物科技股份有限公司 Cultivation of brown crab-flavor mushroom
CN101366346A (en) * 2008-07-31 2009-02-18 芜湖野树林生物科技有限公司 Clear-white gold needle mushroom cultivation method
CN101397223A (en) * 2007-09-29 2009-04-01 孟桂娟 Method for preparing champignon compost
CN101684054A (en) * 2009-03-27 2010-03-31 广东星河生物科技股份有限公司 Formula of culture medium for high yield beech mushrooms and production process

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1939115A (en) * 2005-09-29 2007-04-04 上海丰科生物科技股份有限公司 Cultivation of brown crab-flavor mushroom
CN101397223A (en) * 2007-09-29 2009-04-01 孟桂娟 Method for preparing champignon compost
CN101366346A (en) * 2008-07-31 2009-02-18 芜湖野树林生物科技有限公司 Clear-white gold needle mushroom cultivation method
CN101684054A (en) * 2009-03-27 2010-03-31 广东星河生物科技股份有限公司 Formula of culture medium for high yield beech mushrooms and production process

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104016765B (en) * 2014-05-07 2016-02-10 合肥福泉现代农业科技有限公司 A kind of sawdust is Hypsizygus marmoreus substratum of raw material and preparation method thereof
CN104016761A (en) * 2014-05-07 2014-09-03 合肥福泉现代农业科技有限公司 Hypsizigus marmoreus culture medium and preparation method thereof
CN104016763A (en) * 2014-05-07 2014-09-03 合肥福泉现代农业科技有限公司 Hypsizygus marmoreus culture medium taking peach wood chips as raw materials and preparation method thereof
CN104016765A (en) * 2014-05-07 2014-09-03 合肥福泉现代农业科技有限公司 Hypsizigus marmoreus culture medium with saw dust as raw material and preparation method of hypsizigus marmoreus culture medium
CN104016777A (en) * 2014-05-07 2014-09-03 合肥福泉现代农业科技有限公司 Shiitake culture medium prepared from cottonseed hull and preparation method thereof
CN104016764A (en) * 2014-05-07 2014-09-03 合肥福泉现代农业科技有限公司 Hypsizigus marmoreus culture medium prepared from cotton straw and preparation method thereof
CN104782386A (en) * 2015-03-24 2015-07-22 邬金飞 Hypsizygus marmoreus cultivation method
CN104945051A (en) * 2015-06-19 2015-09-30 桂林健成生物科技开发有限公司 Application of seed coats/embryonic bodies and rhizomes obtained after harvest of sprout seedling vegetable to cultivation of hypsizigus marmoreus
CN105474986A (en) * 2015-11-25 2016-04-13 苏州市经纬农产品有限公司 Method for culturing hypsizygus tessellatus
CN107624504A (en) * 2017-11-07 2018-01-26 福建万辰生物科技股份有限公司 A kind of crab flavour mushroom cultural method
CN108251315A (en) * 2018-03-12 2018-07-06 林勇全 Crab flavour mushroom and its cultural method
CN108251315B (en) * 2018-03-12 2021-03-23 林勇全 Hypsizygus marmoreus and culture method thereof
CN110313346A (en) * 2019-08-05 2019-10-11 江苏华绿生物科技股份有限公司 A kind of corn meal formulation suitable for the plantation of true pleurotus cornucopiae
CN110583368A (en) * 2019-10-10 2019-12-20 西充星河生物科技有限公司 Hypsizigus marmoreus culture medium and application method thereof
CN112293154A (en) * 2020-11-23 2021-02-02 西充星河生物科技有限公司 High-yield cultivation method for white beech mushrooms

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Application publication date: 20130410