CN105412670A - Immunopotentiator for preventing and treating tilapia branchiomycosis and preparation method thereof - Google Patents

Immunopotentiator for preventing and treating tilapia branchiomycosis and preparation method thereof Download PDF

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Publication number
CN105412670A
CN105412670A CN201510971362.XA CN201510971362A CN105412670A CN 105412670 A CN105412670 A CN 105412670A CN 201510971362 A CN201510971362 A CN 201510971362A CN 105412670 A CN105412670 A CN 105412670A
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parts
herba
branchiomycosis
tilapia
immunostimulant
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王芬
李洪
孙明
王亮
宋辉
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Qingdao Haizhiyuan Intelligent Technology Co Ltd
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Qingdao Haizhiyuan Intelligent Technology Co Ltd
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    • A—HUMAN NECESSITIES
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/35—Caprifoliaceae (Honeysuckle family)
    • A61K36/355—Lonicera (honeysuckle)
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    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
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    • A61K35/612—Crustaceans, e.g. crabs, lobsters, shrimps, krill or crayfish; Barnacles
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Abstract

The invention belongs to the technical field of aquaculture, relates to an immunopotentiator for preventing and treating tilapia branchiomycosis, and further relates to a preparation method of the immunopotentiator for preventing and treating the tilapia branchiomycosis. The immunopotentiator for preventing and treating the tilapia branchiomycosis is prepared from, by mass, 32-40 parts of honeysuckle flowers, 25-31 parts of acorus calami, 25-31 parts of agastache rugosus, 24-31 parts of setaria viridis, 22-30 parts of Chinese milk vetch, 21-27 parts of herba portulacae, 20-27 parts of sun euphorbia herb, 20-27 parts of herb of chingma abutilon, 18-26 parts of fructus tribuli, 18-26 parts of herba violae, 19-25 parts of Chinese gall, 17-25 parts of radix alangii, 12-20 parts of nutgrass galingale rhizome, 11-19 parts of mandrake and the like. The immunopotentiator can improve the immunity of tilapia, and the tilapia branchiomycosis can be effectively prevented and treated.

Description

Immunostimulant of control tilapia branchiomycosis and preparation method thereof
Technical field
The invention belongs to technical field of aquaculture, particularly relate to a kind of immunostimulant preventing and treating tilapia branchiomycosis; The invention still further relates to a kind of preparation method of immunostimulant of above-mentioned control tilapia branchiomycosis.
Background technology
Tilapia is former is distributed in temperate zone, the India torrid zone and tropic countries etc.The cultured freshwater fish that the emphasis scientific research that tilapia has become World Water industry is now cultivated, and be described as one of main source of following animal protein.Tilapia originates in Africa, belongs to the tropic fishes of Family Cichlidae, similar with Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis), usually moves in fresh water, also can move in the salt water of different salt content, can survive in lake, river, in the shallow water in pond, have very strong adaptive capacity, and the water less to dissolved oxygen there is extremely strong adaptability.Overwhelming majority tilapia is omnivorousness, often eats aquatic plant and minces.Tilapia also can breed in the narrow and small waters of area.Even can grow in rice terrace.
Branchiomycosis is a kind of common fish disease caused by gill mycete parasitism, and usually occur in the 5-10 month, 6-7 month is popular, and sickness rate can reach 70%-80%, and mortality rate can reach more than 90%, and harm is serious.Pathogen invades gill portion by gill mycete and causes.Its symptom is: gill tissue's religion gill mycete erosion damage, in irregularity white point shape, lose normal cerise, color and luster is pale, and the fish gill be in a bad way covers with flocculence thing, resembles one piece of cotton pellet.Body surface has the congested phenomenon of point-like, and sick fish loses attitude of normally swimming, frightened rear travelling time shake head, morbidity seasonal disease fish is not taken food, dyspnea, and as treated not in time, sick fish will to be obstructed and dead because breathing.Branchiomycosis has morbidity throughout the country.
At present, the method that treatment tilapia branchiomycosis adopts usually is splashed all over pond with the erythromycin of 0.3-0.5ppm, but due to drug residue etc., tilapia after edible traditional method healing is unfavorable for the health of the mankind, and a large amount of erythromycin that uses can cause abuse of antibiotics, is also unfavorable for the sustainable health development of aquaculture industry.
Summary of the invention
An object of the present invention is to provide a kind of immunostimulant preventing and treating tilapia branchiomycosis, its raw material components is green pure natural material, and being added to by the immunostimulant of control tilapia branchiomycosis of the present invention in normal feedstuff can the generation of effective prevention and therapy tilapia branchiomycosis.
In order to solve the problems of the technologies described above, the present invention is by the following technical solutions: a kind of immunostimulant preventing and treating tilapia branchiomycosis, be made up of each component of following masses number: Flos Lonicerae 32-40 part, Rhizoma Acori Graminei 25-31 part, Herba Pogostemonis 25-31 part, Herba Setariae Viridis 24-31 part, Herba Astragali Melilotoidis (Herba Astragali Sinici) 22-30 part, Herba Portulacae 21-27 part, Herba Euphorbiae Helioscopiae 20-27 part, Herba Abutili 20-27 part, Fructus Tribuli 18-26 part, Herba Violae 18-26 part, Galla Chinensis 19-25 part, Radix Alangii 17-25 part, Radix Stephaniae Cepharanthae 17-25 part, Herba Houttuyniae 16-24 part, Fructus Ligustri Lucidi 16-22 part, Herba Saginae Japonicae 16-22 part, Herba Hedyotidis Diffusae 15-25 part, Periostracum Cicadae 13-22 part, conch meal 5-10 part, Pericarpium Citri Reticulatae 12-18 part, Rhizoma Cyperi 12-20 part, Flos Daturae 11-19 part, white vinegar 10-20 part, Bulbus Allii 8-17 part, Mel 7-15 part, dry Fructus Crataegi powder 6-16 part, artemia powder 12-20 part, dry Lumbricus powder 12-20 part, fish oil 10-20 part.
Preferably, the immunostimulant of described control tilapia branchiomycosis, be made up of each component of following masses number: Flos Lonicerae 36 parts, Rhizoma Acori Graminei 29 parts, Herba Pogostemonis 29 parts, Herba Setariae Viridis 27 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 26 parts, Herba Portulacae 24 parts, Herba Euphorbiae Helioscopiae 23 parts, Herba Abutili 24 parts, Fructus Tribuli 22 parts, Herba Violae 22 parts, Galla Chinensis 23 parts, Radix Alangii 20 parts, Radix Stephaniae Cepharanthae 20 parts, Herba Houttuyniae 20 parts, Fructus Ligustri Lucidi 20 parts, Herba Saginae Japonicae 18 parts, Herba Hedyotidis Diffusae 18 parts, Periostracum Cicadae 17 parts, conch meal 8 parts, Pericarpium Citri Reticulatae 15 parts, Rhizoma Cyperi 16 parts, Flos Daturae 14 parts, white vinegar 16 parts, 12 parts, Bulbus Allii, Mel 10 parts, dry Fructus Crataegi powder 10 parts, artemia powder 15 parts, dry Lumbricus powder 14 parts, 17 parts, fish oil.
Preferably, the immunostimulant of described control tilapia branchiomycosis, be made up of each component of following masses number: Flos Lonicerae 36 parts, Rhizoma Acori Graminei 28 parts, Herba Pogostemonis 28 parts, Herba Setariae Viridis 26 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 25 parts, Herba Portulacae 25 parts, Herba Euphorbiae Helioscopiae 24 parts, Herba Abutili 24 parts, Fructus Tribuli 20 parts, Herba Violae 21 parts, Galla Chinensis 22 parts, Radix Alangii 22 parts, Radix Stephaniae Cepharanthae 21 parts, Herba Houttuyniae 20 parts, Fructus Ligustri Lucidi 18 parts, Herba Saginae Japonicae 20 parts, Herba Hedyotidis Diffusae 20 parts, Periostracum Cicadae 18 parts, conch meal 7 parts, Pericarpium Citri Reticulatae 14 parts, Rhizoma Cyperi 15 parts, Flos Daturae 15 parts, white vinegar 15 parts, 12 parts, Bulbus Allii, Mel 12 parts, dry Fructus Crataegi powder 9 parts, artemia powder 16 parts, dry Lumbricus powder 16 parts, 15 parts, fish oil.
Each component effect of the present invention is resolved as follows:
Flos Lonicerae has another name called Radix Ophiopogonis, property sweet cold fragrance, clearing away heat with drugs sweet in flavor and cold in nature and not injuring one's stomach, fragrance thoroughly reaches and can be eliminating evil, and Flos Lonicerae can dispelling wind-heat, also kind removing summer-heat blood poison, for various febrile disease, as fever of the body, dermexanthesis, send out the diseases such as speckle, pyretic toxicity carbuncle sore, laryngopharynx swelling and pain, equal Be very effective.Rhizoma Acori Graminei can be dispelled epidemic disease Fructus Alpiniae Oxyphyllae, damp eliminating removing toxic substances, building body, the deinsectization of combustion Rhizoma Acori Graminei energy anophelifuge.The removing dampness of Herba Pogostemonis energy is amusing, ward off dirty and in, expelling summer-heat, delivers, can be used for dampness taste, distension and fullness in the abdomen, hygropyrexia from the beginning of, vomit, have loose bowels, the disease such as heat-damp in summer, fever with chills, fever with aversion to cold, chest distress.Herba Setariae Viridis can clearing away heat-damp and promoting diuresis, detumescence; Dispelling pathogenic wind for improving eyesight; Removing toxic substances; Parasite killing, main anemopyretic cold; Jaundice; Infantile malnutrition; Dysentery; Difficulty and pain in micturition; The puckery pain of conjunctival congestion; Conjunctival congestion and swelling pain; Carbuncle; Verruca vulgaris; Skin ulcer tinea.Herba Astragali Melilotoidis (Herba Astragali Sinici) has dispelling pathogenic wind for improving eyesight, invigorating the spleen and benefiting QI, the effect of removing toxic substances pain relieving.Herba Portulacae is Portulacaceae annual herb plant, herb hyoscine, has clearing away heat-damp and promoting diuresis, removing toxic substances and promoting subsidence of swelling, antiinflammatory, quenches the thirst, diuresis.Herba Euphorbiae Helioscopiae acrid in the mouth, hardship, cold nature, line water detumescence, preventing phlegm from forming and stopping coughing, removing toxic substances parasite killing, cures mainly edematous asthma, and phlegm retention is breathed with cough malaria, bacillary dysentery, scrofula, Tuberculous fistula, osteomyelitis.The annual suffruticose draft of Herba Abutili, stem branch by pubescence, bitter in the mouth, property is put down, can clearing away heat-damp and promoting diuresis, removing toxic substances, and nebula removing, can be used for corneal nebula, dysentery, carbuncle; Urine leaching pain, dysentery, can detoxify, dispel the wind, and also can be used for treating skin sore, dysentery, otitis media, tinnitus, deaf, joint aches.Fructus Tribuli property a surname is logical soon just, and can capacity disappear, the stagnant gas of row liver spleen, many clothes take Fructus Tribuli for a long time, have stagnant merit.Herba Violae bitter in the mouth, pungent, cold in nature, can GUIXIN, lung meridian, have heat-clearing and toxic substances removing, removing heat from blood is subsided a swelling, and the effect of clearing away heat-damp and promoting diuresis, cures mainly furuncle, carbuncle, scrofula, jaundice, dysentery, diarrhoea, conjunctival congestion, sore throat, venom.Galla Chinensis is cold in nature, sour in the mouth, puckery, has the effects such as pathogenic fire reducing of astringing the lung, relieving diarrhea with astringents, arresting sweating hemostasis, removing dampness sore, cures mainly the diseases such as chronic cough of deficiency lung, chronic diarrhea chronic dysentery, hemorrhoidal bleeding of having blood in stool, carbuncle sore tumefacting virus, Skin wet and rotten.Radix Alangii acrid in the mouth, hardship, warm in nature, poisonous, can expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral, eliminating stasis to stop pain.Radix Stephaniae Cepharanthae is Menispermaceae stephania plant, is used as medicine with tuber, can heat-clearing and toxic substances removing, cooling blood for hemostasis, and dissipating blood stasis for subsidence of swelling, can be used for acute hepatitis, bacillary dysentery, acute appendicitis, stomachache, internal hemorrhage, traumatic injury, venom; Mumps is controlled in external, lymphadenitis, neurodermatitis.Herba Houttuyniae acrid in the mouth, cold nature; Return lung, bladder, large intestine channel, have heat-clearing and toxic substances removing, reduce phlegm removing pus and relieving carbuncle, and the effect that inducing diuresis to remove edema is treating stranguria, cures mainly dyspnea and cough due to lung-heat, lung abscess vomiting pus, throat moth, hematodiarrhoea, malaria, edema, carbuncle sore tumefacting virus, pyretic stranguria, eczema, the diseases such as proctoptosis.Fructus Ligustri Lucidi odorless, sweet in the mouth, micro-bitterness, cure mainly yin asthenia generating intrinsic heat, dizzy, order flower, tinnitus, soreness of the waist and knees, early whitening of beard and hair.Herba Saginae Japonicae can be used for treating dermatitis rhus, tinea alba, carbuncle, scrofula, dental caries, children's's infantile dyspepsia, internal traumatic injury.Herba Hedyotidis Diffusae bitter in the mouth, light, cold in nature, primary efficacy is heat-clearing and toxic substances removing, medicinal powder for relieving pain knot.Periostracum Cicadae energy dispelling wind-heat, rash sore-throat relieving, removing nebula to improve visual acuity, to expelling wind and relieving convulsion.Not only containing a large amount of calcium in conch meal, but also containing necessary trace element in poultry body: phosphorus, manganese, zinc, copper, ferrum, potassium, magnesium etc.Pericarpium Citri Reticulatae has the effects such as antiinflammatory, antiulcer, function of gallbladder promoting, also can control taste uncomfortable.Rhizoma Cyperi can be used for treating stagnation of QI due to depression of the liver, and distending pain in the chest and hypochondrium, hernia pain, distending pain of the breast, stagnation of QI in spleen and stomach, gastral cavity abdomen painful abdominal mass is vexed, distension pain.Flos Daturae can control infantile convulsion and asthma of cold-type, can be used for antitussive analgesia.White vinegar can whet the appetite, and promotes the secretion of saliva and gastric juice, can help digest absorption, make honey stomach, promoting digestion and removing stagnation, and white vinegar has well antibacterial and bactericidal action.Bulbus Allii can be detoxified parasite killing, can reducing swelling and alleviating pain, antidiarrheal dysentery relieving, bactericidal.Mel can loosening bowel to relieve constipation, strength can be made up rapidly, eliminate tired out, build up one's resistance to disease, can antibacterial sterilizing, and feedstuff mouthfeel can be improved.Fructus Crataegi can improve mouthfeel, and can spleen benefiting and stimulating the appetite, relieving dyspepsia, phlegm reduction of blood circulation promoting, antiinflammatory antibacterial.Artemia contains rich in protein, and unsaturated fatty acid content is high, is the living body bait of Fish the best.Lumbricus is nutritious, and has food calling effect to tilapia.Fish oil can not only provide energy for tilapia and have food calling effect.
Another object of the present invention is to provide a kind of preparation method of immunostimulant of above-mentioned control tilapia branchiomycosis, and the preparation method of the immunostimulant of this control tilapia branchiomycosis comprises the following steps:
(1) take Flos Lonicerae according to proportioning, add the purified water that parts by weight are Flos Lonicerae parts by weight 20 times, 4-6h is soaked in 70-80 DEG C of water-bath, filters to obtain infusion;
(2) Rhizoma Acori Graminei, Herba Pogostemonis, Herba Setariae Viridis, Herba Astragali Melilotoidis (Herba Astragali Sinici), Herba Portulacae, Herba Euphorbiae Helioscopiae, Herba Abutili, Fructus Tribuli, Herba Violae, Herba Houttuyniae, Herba Saginae Japonicae, Herba Hedyotidis Diffusae, Pericarpium Citri Reticulatae and Bulbus Allii is taken according to proportioning, gross weight is designated as 1 part, add 6 parts of purified water, 20-30 DEG C is soaked 6-9h, obtains soak;
(3) boil 2h after the soak that step (2) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 3 parts in medical material, continue to boil 1h after being heated to boiling, merge twice fried liquid and be extracting solution;
(4) take Galla Chinensis, Radix Alangii, Radix Stephaniae Cepharanthae, Fructus Ligustri Lucidi, Periostracum Cicadae, conch meal, Rhizoma Cyperi and Flos Daturae respectively according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(5) grounds travel that extracting solution step (3) obtained and step (4) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(6) take white vinegar, Mel, dry Fructus Crataegi powder, artemia powder, dry Lumbricus powder and fish oil according to proportioning, mix homogeneously with the infusion of step (1) gained, the extract of step (5) gained, obtain object.
The preparation method of the Fructus Crataegi powder of described drying is: cleaned up by Fructus Crataegi, grinds on ice in freezer dryer after lyophilization to finish-drying, after 50 mesh sieves, obtains dry Fructus Crataegi powder.Cryodesiccated advantage can not make the active substance inactivation contained by Fructus Crataegi in dry run, because constantly stir produces heat, to keep temperature, protective tissue when the advantage of grinding is and reduces grinding on ice.
The preparation method of the Lumbricus powder of described drying is: the Lumbricus lyophilization cleaned up ground on ice to finish-drying, after 50 mesh sieves, obtains dry Lumbricus powder.Cryodesiccated advantage can not make the active substance inactivation contained by Lumbricus in dry run, because constantly stir produces heat, to keep temperature, protective tissue when the advantage of grinding is and reduces grinding on ice.
Usage and dosage: throw something and feed after the immunostimulant of control tilapia branchiomycosis of the present invention is mixed homogeneously with the weight ratio of 1:30-1:10 with commercially available normal feedstuff, preferably, the immunostimulant of control tilapia branchiomycosis of the present invention and the weight ratio of commercially available normal feedstuff are 1:20.
In preparation method, the object that Flos Lonicerae is soaked 4-6h 70-80 DEG C of water-bath is fully separated out by the effective ingredient of Flos Lonicerae, by Rhizoma Acori Graminei, Herba Pogostemonis, Herba Setariae Viridis, Herba Astragali Melilotoidis (Herba Astragali Sinici), Herba Portulacae, Herba Euphorbiae Helioscopiae, Herba Abutili, Fructus Tribuli, Herba Violae, Herba Houttuyniae, Herba Saginae Japonicae, Herba Hedyotidis Diffusae, Pericarpium Citri Reticulatae and Bulbus Allii purified water soaking a period of time, medical material deliquescing, cell expansion, effective ingredient is easily fried, and medical material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medical material surface, protein coagulating, the capillary channel on blocking medical material surface, the moisture when decocting is made to enter not go, effective ingredient is not easily fried, affect curative effect of medication, decoction can change the irritation effect of medicine, relatively mildly act on tilapia, promote that tilapia is to the absorption of ingredient, by Galla Chinensis, Radix Alangii, Radix Stephaniae Cepharanthae, Fructus Ligustri Lucidi, Periostracum Cicadae, conch meal, Rhizoma Cyperi with Flos Daturae grinding can make the starch in medical material, protein separates out more fully at subsequent step, supercritical extraction step retains the effective ingredient of each component in the present invention to greatest extent.
The fish oil contained in immunostimulant due to control tilapia branchiomycosis of the present invention has food calling effect, artemia powder and Lumbricus nutritious, delicious flavour, Fructus Crataegi can improve the overall mouthfeel of feedstuff and energy spleen benefiting and stimulating the appetite, relieving dyspepsia, Mel can improve mouthfeel and strengthen the immunity of tilapia, Mel, dry Fructus Crataegi powder, artemia powder, dry Lumbricus powder and fish oil have synergism, therefore the immunostimulant of control tilapia branchiomycosis of the present invention can improve the mouthfeel of normal feedstuff, stimulate the taste bud of tilapia, induction tilapia ingests and adds the normal feedstuff of immunostimulant of the present invention, therefore tilapia immunity and resistance can be strengthened in immunostimulant of the present invention, composition branchiomycosis to remarkable inhibition can enter in tilapia body, and play it and act on accordingly.
The immunostimulant of control tilapia branchiomycosis of the present invention is the etiopathogenesis according to branchiomycosis, the suitable component of selection, and is organically combined, and has significant cooperative synergism effect, plays the function of each component to greatest extent.The component of the immunostimulant of control tilapia branchiomycosis of the present invention is environmental protection raw material, nontoxic to environment, not containing antibiotic, can not affect the healthy of people after edible tilapia of having cured, and immunostimulant of the present invention is good to the curative effect of tilapia branchiomycosis, cure rate is high, make the survival rate of ill tilapia to more than 90%, after medication 2-3 days, tilapia starts progressively to recover to ingest, and ingestion frequency improves.
The present invention uses safer, little to tilapia damage, after using the immunostimulant for the treatment of tilapia branchiomycosis of the present invention; tilapia Health restoration is fast, medication 2-6 days, and tilapia can recover normal activity; idle fund is strong and vigorous, and the gill filament recovers normal, ingests completely normal.And the immunostimulant of control tilapia branchiomycosis of the present invention effectively can also prevent the generation of tilapia branchiomycosis.
Compared with prior art, the invention has the beneficial effects as follows:
(1) Flos Lonicerae has another name called Radix Ophiopogonis, property sweet cold fragrance, clearing away heat with drugs sweet in flavor and cold in nature and not injuring one's stomach, fragrance thoroughly reaches and can be eliminating evil, and Flos Lonicerae can dispelling wind-heat, also kind removing summer-heat blood poison, for various febrile disease, as fever of the body, dermexanthesis, send out the diseases such as speckle, pyretic toxicity carbuncle sore, laryngopharynx swelling and pain, equal Be very effective.Rhizoma Acori Graminei can be dispelled epidemic disease Fructus Alpiniae Oxyphyllae, damp eliminating removing toxic substances, building body, the deinsectization of combustion Rhizoma Acori Graminei energy anophelifuge.The removing dampness of Herba Pogostemonis energy is amusing, ward off dirty and in, expelling summer-heat, delivers, can be used for dampness taste, distension and fullness in the abdomen, hygropyrexia from the beginning of, vomit, have loose bowels, the disease such as heat-damp in summer, fever with chills, fever with aversion to cold, chest distress.Herba Setariae Viridis can clearing away heat-damp and promoting diuresis, detumescence; Dispelling pathogenic wind for improving eyesight; Removing toxic substances; Parasite killing, main anemopyretic cold; Jaundice; Infantile malnutrition; Dysentery; Difficulty and pain in micturition; The puckery pain of conjunctival congestion; Conjunctival congestion and swelling pain; Carbuncle; Verruca vulgaris; Skin ulcer tinea.Herba Astragali Melilotoidis (Herba Astragali Sinici) has dispelling pathogenic wind for improving eyesight, invigorating the spleen and benefiting QI, the effect of removing toxic substances pain relieving.Herba Portulacae is Portulacaceae annual herb plant, herb hyoscine, has clearing away heat-damp and promoting diuresis, removing toxic substances and promoting subsidence of swelling, antiinflammatory, quenches the thirst, diuresis.Herba Euphorbiae Helioscopiae acrid in the mouth, hardship, cold nature, line water detumescence, preventing phlegm from forming and stopping coughing, removing toxic substances parasite killing, cures mainly edematous asthma, and phlegm retention is breathed with cough malaria, bacillary dysentery, scrofula, Tuberculous fistula, osteomyelitis.The annual suffruticose draft of Herba Abutili, stem branch by pubescence, bitter in the mouth, property is put down, can clearing away heat-damp and promoting diuresis, removing toxic substances, and nebula removing, can be used for corneal nebula, dysentery, carbuncle; Urine leaching pain, dysentery, can detoxify, dispel the wind, and also can be used for treating skin sore, dysentery, otitis media, tinnitus, deaf, joint aches.Fructus Tribuli property a surname is logical soon just, and can capacity disappear, the stagnant gas of row liver spleen, many clothes take Fructus Tribuli for a long time, have stagnant merit.Herba Violae bitter in the mouth, pungent, cold in nature, can GUIXIN, lung meridian, have heat-clearing and toxic substances removing, removing heat from blood is subsided a swelling, and the effect of clearing away heat-damp and promoting diuresis, cures mainly furuncle, carbuncle, scrofula, jaundice, dysentery, diarrhoea, conjunctival congestion, sore throat, venom.Galla Chinensis is cold in nature, sour in the mouth, puckery, has the effects such as pathogenic fire reducing of astringing the lung, relieving diarrhea with astringents, arresting sweating hemostasis, removing dampness sore, cures mainly the diseases such as chronic cough of deficiency lung, chronic diarrhea chronic dysentery, hemorrhoidal bleeding of having blood in stool, carbuncle sore tumefacting virus, Skin wet and rotten.Radix Alangii acrid in the mouth, hardship, warm in nature, poisonous, can expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral, eliminating stasis to stop pain.Radix Stephaniae Cepharanthae is Menispermaceae stephania plant, is used as medicine with tuber, can heat-clearing and toxic substances removing, cooling blood for hemostasis, and dissipating blood stasis for subsidence of swelling, can be used for acute hepatitis, bacillary dysentery, acute appendicitis, stomachache, internal hemorrhage, traumatic injury, venom; Mumps is controlled in external, lymphadenitis, neurodermatitis.Herba Houttuyniae acrid in the mouth, cold nature; Return lung, bladder, large intestine channel, have heat-clearing and toxic substances removing, reduce phlegm removing pus and relieving carbuncle, and the effect that inducing diuresis to remove edema is treating stranguria, cures mainly dyspnea and cough due to lung-heat, lung abscess vomiting pus, throat moth, hematodiarrhoea, malaria, edema, carbuncle sore tumefacting virus, pyretic stranguria, eczema, the diseases such as proctoptosis.Fructus Ligustri Lucidi odorless, sweet in the mouth, micro-bitterness, cure mainly yin asthenia generating intrinsic heat, dizzy, order flower, tinnitus, soreness of the waist and knees, early whitening of beard and hair.Herba Saginae Japonicae can be used for treating dermatitis rhus, tinea alba, carbuncle, scrofula, dental caries, children's's infantile dyspepsia, internal traumatic injury.Herba Hedyotidis Diffusae bitter in the mouth, light, cold in nature, primary efficacy is heat-clearing and toxic substances removing, medicinal powder for relieving pain knot.Periostracum Cicadae energy dispelling wind-heat, rash sore-throat relieving, removing nebula to improve visual acuity, to expelling wind and relieving convulsion.Not only containing a large amount of calcium in conch meal, but also containing necessary trace element in poultry body: phosphorus, manganese, zinc, copper, ferrum, potassium, magnesium etc.Pericarpium Citri Reticulatae has the effects such as antiinflammatory, antiulcer, function of gallbladder promoting, also can control taste uncomfortable.Rhizoma Cyperi can be used for treating stagnation of QI due to depression of the liver, and distending pain in the chest and hypochondrium, hernia pain, distending pain of the breast, stagnation of QI in spleen and stomach, gastral cavity abdomen painful abdominal mass is vexed, distension pain.Flos Daturae can control infantile convulsion and asthma of cold-type, can be used for antitussive analgesia.White vinegar can whet the appetite, and promotes the secretion of saliva and gastric juice, can help digest absorption, make honey stomach, promoting digestion and removing stagnation, and white vinegar has well antibacterial and bactericidal action.Bulbus Allii can be detoxified parasite killing, can reducing swelling and alleviating pain, antidiarrheal dysentery relieving, bactericidal.Mel can loosening bowel to relieve constipation, strength can be made up rapidly, eliminate tired out, build up one's resistance to disease, can antibacterial sterilizing, and feedstuff mouthfeel can be improved.Fructus Crataegi can improve mouthfeel, and can spleen benefiting and stimulating the appetite, relieving dyspepsia, phlegm reduction of blood circulation promoting, antiinflammatory antibacterial.Artemia contains rich in protein, and unsaturated fatty acid content is high, is the living body bait of Fish the best.Lumbricus is nutritious, and has food calling effect to tilapia.Fish oil can not only provide energy for tilapia and have food calling effect.
(2) immunostimulant of control tilapia branchiomycosis of the present invention, is the raw material components selected according to the etiopathogenesis of branchiomycosis, and is organically combined, have significant cooperative synergism effect, played the function of each component to greatest extent.
(3), in preparation method, the object that Flos Lonicerae is soaked 4-6h 70-80 DEG C of water-bath is fully separated out by the effective ingredient of Flos Lonicerae, by Rhizoma Acori Graminei, Herba Pogostemonis, Herba Setariae Viridis, Herba Astragali Melilotoidis (Herba Astragali Sinici), Herba Portulacae, Herba Euphorbiae Helioscopiae, Herba Abutili, Fructus Tribuli, Herba Violae, Herba Houttuyniae, Herba Saginae Japonicae, Herba Hedyotidis Diffusae, Pericarpium Citri Reticulatae and Bulbus Allii purified water soaking a period of time, medical material deliquescing, cell expansion, effective ingredient is easily fried, and medical material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medical material surface, protein coagulating, the capillary channel on blocking medical material surface, the moisture when decocting is made to enter not go, effective ingredient is not easily fried, affect curative effect of medication, decoction can change the irritation effect of medicine, relatively mildly act on tilapia, promote that tilapia is to the absorption of ingredient, by Galla Chinensis, Radix Alangii, Radix Stephaniae Cepharanthae, Fructus Ligustri Lucidi, Periostracum Cicadae, conch meal, Rhizoma Cyperi with Flos Daturae grinding can make the starch in medical material, protein separates out more fully at subsequent step, supercritical extraction step retains the effective ingredient of each component in the present invention to greatest extent.
(4) fish oil contained in the immunostimulant due to control tilapia branchiomycosis of the present invention has food calling effect, artemia powder and Lumbricus nutritious, delicious flavour, Fructus Crataegi can improve the overall mouthfeel of feedstuff and energy spleen benefiting and stimulating the appetite, relieving dyspepsia, Mel can improve mouthfeel and strengthen the immunity of tilapia, Mel, dry Fructus Crataegi powder, artemia powder, dry Lumbricus powder and fish oil have synergism, therefore the immunostimulant of control tilapia branchiomycosis of the present invention can improve the mouthfeel of normal feedstuff, stimulate the taste bud of tilapia, induction tilapia ingests and adds the normal feedstuff of immunostimulant of the present invention, therefore tilapia immunity and resistance can be strengthened in immunostimulant of the present invention, composition branchiomycosis to remarkable inhibition can enter in tilapia body, and play it and act on accordingly.
(5) component of the immunostimulant of control tilapia branchiomycosis of the present invention is environmental protection raw material, nontoxic to environment, not containing antibiotic, can not affect the healthy of people after edible tilapia of having cured, and immunostimulant of the present invention is good to the curative effect of tilapia branchiomycosis, cure rate is high, make the survival rate of ill tilapia to more than 90%, after medication 2-3 days, tilapia starts progressively to recover to ingest, and ingestion frequency improves.And the immunostimulant of control tilapia branchiomycosis of the present invention effectively can also prevent the generation of tilapia branchiomycosis.
Detailed description of the invention
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.
Embodiment 1
A kind of immunostimulant preventing and treating tilapia branchiomycosis, be made up of each component of following masses number: Flos Lonicerae 40 parts, Rhizoma Acori Graminei 31 parts, Herba Pogostemonis 31 parts, Herba Setariae Viridis 30 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 30 parts, Herba Portulacae 27 parts, Herba Euphorbiae Helioscopiae 27 parts, Herba Abutili 26 parts, Fructus Tribuli 26 parts, Herba Violae 26 parts, Galla Chinensis 25 parts, Radix Alangii 25 parts, Radix Stephaniae Cepharanthae 24 parts, Herba Houttuyniae 24 parts, Fructus Ligustri Lucidi 22 parts, Herba Saginae Japonicae 22 parts, Herba Hedyotidis Diffusae 25 parts, Periostracum Cicadae 22 parts, conch meal 10 parts, Pericarpium Citri Reticulatae 18 parts, Rhizoma Cyperi 18 parts, Flos Daturae 19 parts, white vinegar 20 parts, 15 parts, Bulbus Allii, Mel 15 parts, dry Fructus Crataegi powder 15 parts, artemia powder 20 parts, dry Lumbricus powder 18 parts, 18 parts, fish oil.
The preparation method of the immunostimulant of above-mentioned control tilapia branchiomycosis comprises the following steps:
(1) take Flos Lonicerae according to proportioning, add the purified water that parts by weight are 20 times of Flos Lonicerae parts by weight, 4-6h is soaked in 70-80 DEG C of water-bath, filters to obtain infusion;
(2) Rhizoma Acori Graminei, Herba Pogostemonis, Herba Setariae Viridis, Herba Astragali Melilotoidis (Herba Astragali Sinici), Herba Portulacae, Herba Euphorbiae Helioscopiae, Herba Abutili, Fructus Tribuli, Herba Violae, Herba Houttuyniae, Herba Saginae Japonicae, Herba Hedyotidis Diffusae, Pericarpium Citri Reticulatae and Bulbus Allii is taken according to proportioning, gross weight is designated as 1 part, add 6 parts of purified water, 20-30 DEG C is soaked 6-9h, obtains soak;
(3) boil 2h after the soak that step (2) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 3 parts in medical material, continue to boil 1h after being heated to boiling, merge twice fried liquid and be extracting solution;
(4) take Galla Chinensis, Radix Alangii, Radix Stephaniae Cepharanthae, Fructus Ligustri Lucidi, Periostracum Cicadae, conch meal, Rhizoma Cyperi and Flos Daturae respectively according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(5) grounds travel that extracting solution step (3) obtained and step (4) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(6) take white vinegar, Mel, dry Fructus Crataegi powder, artemia powder, dry Lumbricus powder and fish oil according to proportioning, mix homogeneously with the infusion of step (1) gained, the extract of step (5) gained, obtain object.
The preparation method of the Fructus Crataegi powder of described drying is: cleaned up by Fructus Crataegi, grinds on ice in freezer dryer after lyophilization to finish-drying, after 50 mesh sieves, obtains dry Fructus Crataegi powder.
The preparation method of the Lumbricus powder of described drying is: the Lumbricus lyophilization cleaned up ground on ice to finish-drying, after 50 mesh sieves, obtains dry Lumbricus powder.
Embodiment 2
A kind of immunostimulant preventing and treating tilapia branchiomycosis, be made up of each component of following masses number: Flos Lonicerae 38 parts, Rhizoma Acori Graminei 31 parts, Herba Pogostemonis 30 parts, Herba Setariae Viridis 31 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 28 parts, Herba Portulacae 27 parts, Herba Euphorbiae Helioscopiae 26 parts, Herba Abutili 27 parts, Fructus Tribuli 24 parts, Herba Violae 24 parts, Galla Chinensis 24 parts, Radix Alangii 24 parts, Radix Stephaniae Cepharanthae 25 parts, Herba Houttuyniae 22 parts, Fructus Ligustri Lucidi 21 parts, Herba Saginae Japonicae 22 parts, Herba Hedyotidis Diffusae 22 parts, Periostracum Cicadae 20 parts, conch meal 8 parts, Pericarpium Citri Reticulatae 16 parts, Rhizoma Cyperi 20 parts, Flos Daturae 18 parts, white vinegar 18 parts, 17 parts, Bulbus Allii, Mel 14 parts, dry Fructus Crataegi powder 16 parts, artemia powder 16 parts, dry Lumbricus powder 20 parts, 20 parts, fish oil.
The preparation method of the immunostimulant of the control tilapia branchiomycosis of the present embodiment is with embodiment 1.
Embodiment 3
A kind of immunostimulant preventing and treating tilapia branchiomycosis, be made up of each component of following masses number: Flos Lonicerae 32 parts, Rhizoma Acori Graminei 26 parts, Herba Pogostemonis 26 parts, Herba Setariae Viridis 24 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 22 parts, Herba Portulacae 20 parts, Herba Euphorbiae Helioscopiae 20 parts, Herba Abutili 20 parts, Fructus Tribuli 18 parts, Herba Violae 18 parts, Galla Chinensis 19 parts, Radix Alangii 17 parts, Radix Stephaniae Cepharanthae 17 parts, Herba Houttuyniae 16 parts, Fructus Ligustri Lucidi 16 parts, Herba Saginae Japonicae 17 parts, Herba Hedyotidis Diffusae 15 parts, Periostracum Cicadae 13 parts, conch meal 5 parts, Pericarpium Citri Reticulatae 12 parts, Rhizoma Cyperi 14 parts, Flos Daturae 11 parts, white vinegar 12 parts, 10 parts, Bulbus Allii, Mel 8 parts, dry Fructus Crataegi powder 6 parts, artemia powder 12 parts, dry Lumbricus powder 14 parts, 12 parts, fish oil.
The preparation method of the immunostimulant of the control tilapia branchiomycosis of the present embodiment is with embodiment 1.
Embodiment 4
A kind of immunostimulant preventing and treating tilapia branchiomycosis, be made up of each component of following masses number: Flos Lonicerae 33 parts, Rhizoma Acori Graminei 25 parts, Herba Pogostemonis 25 parts, Herba Setariae Viridis 24 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 24 parts, Herba Portulacae 21 parts, Herba Euphorbiae Helioscopiae 21 parts, Herba Abutili 21 parts, Fructus Tribuli 18 parts, Herba Violae 20 parts, Galla Chinensis 19 parts, Radix Alangii 19 parts, Radix Stephaniae Cepharanthae 19 parts, Herba Houttuyniae 18 parts, Fructus Ligustri Lucidi 16 parts, Herba Saginae Japonicae 16 parts, Herba Hedyotidis Diffusae 18 parts, Periostracum Cicadae 15 parts, conch meal 6 parts, Pericarpium Citri Reticulatae 14 parts, Rhizoma Cyperi 12 parts, Flos Daturae 13 parts, white vinegar 10 parts, 8 parts, Bulbus Allii, Mel 7 parts, dry Fructus Crataegi powder 8 parts, artemia powder 13 parts, dry Lumbricus powder 12 parts, 10 parts, fish oil.
The preparation method of the immunostimulant of the control tilapia branchiomycosis of the present embodiment is with embodiment 1.
Embodiment 5
A kind of immunostimulant preventing and treating tilapia branchiomycosis, be made up of each component of following masses number: Flos Lonicerae 36 parts, Rhizoma Acori Graminei 29 parts, Herba Pogostemonis 29 parts, Herba Setariae Viridis 27 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 26 parts, Herba Portulacae 24 parts, Herba Euphorbiae Helioscopiae 23 parts, Herba Abutili 24 parts, Fructus Tribuli 22 parts, Herba Violae 22 parts, Galla Chinensis 23 parts, Radix Alangii 20 parts, Radix Stephaniae Cepharanthae 20 parts, Herba Houttuyniae 20 parts, Fructus Ligustri Lucidi 20 parts, Herba Saginae Japonicae 18 parts, Herba Hedyotidis Diffusae 18 parts, Periostracum Cicadae 17 parts, conch meal 8 parts, Pericarpium Citri Reticulatae 15 parts, Rhizoma Cyperi 16 parts, Flos Daturae 14 parts, white vinegar 16 parts, 12 parts, Bulbus Allii, Mel 10 parts, dry Fructus Crataegi powder 10 parts, artemia powder 15 parts, dry Lumbricus powder 14 parts, 17 parts, fish oil.
The preparation method of the immunostimulant of the control tilapia branchiomycosis of the present embodiment is with embodiment 1.
Embodiment 6
A kind of immunostimulant preventing and treating tilapia branchiomycosis, be made up of each component of following masses number: Flos Lonicerae 36 parts, Rhizoma Acori Graminei 28 parts, Herba Pogostemonis 28 parts, Herba Setariae Viridis 26 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 25 parts, Herba Portulacae 25 parts, Herba Euphorbiae Helioscopiae 24 parts, Herba Abutili 24 parts, Fructus Tribuli 20 parts, Herba Violae 21 parts, Galla Chinensis 22 parts, Radix Alangii 22 parts, Radix Stephaniae Cepharanthae 21 parts, Herba Houttuyniae 20 parts, Fructus Ligustri Lucidi 18 parts, Herba Saginae Japonicae 20 parts, Herba Hedyotidis Diffusae 20 parts, Periostracum Cicadae 18 parts, conch meal 7 parts, Pericarpium Citri Reticulatae 14 parts, Rhizoma Cyperi 15 parts, Flos Daturae 15 parts, white vinegar 15 parts, 12 parts, Bulbus Allii, Mel 12 parts, dry Fructus Crataegi powder 9 parts, artemia powder 16 parts, dry Lumbricus powder 16 parts, 15 parts, fish oil.
The preparation method of the immunostimulant of the control tilapia branchiomycosis of the present embodiment is with embodiment 1.
Experimental result
Contrast experiment 1
Test site is selected in fishing ground, Qingdao, in order to verify therapeutic effect of the present invention, is testing the tilapia suffering from branchiomycosis:
The process of matched group is as follows: every day, erythromycin to be splashed ill pond (erythromycin concentration in pond waters is 0.4ppm) all over pond, until the recovery from illness of sick fish.
The process of experiment A group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the immunostimulant of the control tilapia branchiomycosis of the embodiment of the present invention 1, until the recovery from illness of sick fish.
The process of experiment B group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the immunostimulant of the control tilapia branchiomycosis of the embodiment of the present invention 2, until the recovery from illness of sick fish.
The process of experiment C group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the immunostimulant of the control tilapia branchiomycosis of the embodiment of the present invention 3, until the recovery from illness of sick fish.
The process of experiment D group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the immunostimulant of the control tilapia branchiomycosis of the embodiment of the present invention 4, until the recovery from illness of sick fish.
The process of experiment E group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the immunostimulant of the control tilapia branchiomycosis of the embodiment of the present invention 5, until the recovery from illness of sick fish.
The process of experiment F group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the immunostimulant of the control tilapia branchiomycosis of the embodiment of the present invention 6, until the recovery from illness of sick fish.
Experimental result is in table 1:
The immunostimulant therapeutic effect of table 1 control of the present invention tilapia branchiomycosis
Experimental result shows: the immunostimulant of control tilapia branchiomycosis of the present invention is the etiopathogenesis according to branchiomycosis, the suitable component of selection, and is organically combined, and has significant cooperative synergism effect, plays the function of each component to greatest extent.The component of the immunostimulant of control tilapia branchiomycosis of the present invention is environmental protection raw material, nontoxic to environment, not containing antibiotic, is conducive to the sustainable health development of culture fishery; Can not affect the healthy of people after edible tilapia of having cured, and immunostimulant of the present invention is good to the curative effect of tilapia branchiomycosis, cure rate is high, and the cycle of curing is short.
Contrast experiment 2
Normally to raise group for blank group.Experimental group process is as follows:
Experiment a group: throw something and feed after the immunostimulant of the control tilapia branchiomycosis of the embodiment of the present invention 1 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
Experiment b group: throw something and feed after the immunostimulant of the control tilapia branchiomycosis of the embodiment of the present invention 2 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
Experiment c group: throw something and feed after the immunostimulant of the control tilapia branchiomycosis of the embodiment of the present invention 3 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
Experiment d group: throw something and feed after the immunostimulant of the control tilapia branchiomycosis of the embodiment of the present invention 4 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
Experiment e group: throw something and feed after the immunostimulant of the control tilapia branchiomycosis of the embodiment of the present invention 5 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
Experiment f group: throw something and feed after the immunostimulant of the control tilapia branchiomycosis of the embodiment of the present invention 6 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
When experiment starts, each processed group puts into healthy tilapia 10, after raising 20 days, adds the tilapia that 1 is suffered from branchiomycosis in each processed group by above-mentioned process, observes and records infection state after one week.The results are shown in Table 2:
The effect of the immunostimulant prevention branchiomycosis of table 2 control of the present invention tilapia branchiomycosis
From table 2, the immunostimulant of control tilapia branchiomycosis of the present invention effectively can reduce the incidence rate of tilapia branchiomycosis, can improve the immunity of tilapia, has good preventive effect to branchiomycosis.
The above is only the preferred embodiment of the present invention, is noted that for those skilled in the art, and under the premise without departing from the principles of the invention, can also make some improvement, these improvement should be considered as protection scope of the present invention.

Claims (8)

1. prevent and treat the immunostimulant of tilapia branchiomycosis for one kind, it is characterized in that: be made up of each component of following masses number: Flos Lonicerae 32-40 part, Rhizoma Acori Graminei 25-31 part, Herba Pogostemonis 25-31 part, Herba Setariae Viridis 24-31 part, Herba Astragali Melilotoidis (Herba Astragali Sinici) 22-30 part, Herba Portulacae 21-27 part, Herba Euphorbiae Helioscopiae 20-27 part, Herba Abutili 20-27 part, Fructus Tribuli 18-26 part, Herba Violae 18-26 part, Galla Chinensis 19-25 part, Radix Alangii 17-25 part, Radix Stephaniae Cepharanthae 17-25 part, Herba Houttuyniae 16-24 part, Fructus Ligustri Lucidi 16-22 part, Herba Saginae Japonicae 16-22 part, Herba Hedyotidis Diffusae 15-25 part, Periostracum Cicadae 13-22 part, conch meal 5-10 part, Pericarpium Citri Reticulatae 12-18 part, Rhizoma Cyperi 12-20 part, Flos Daturae 11-19 part, white vinegar 10-20 part, Bulbus Allii 8-17 part, Mel 7-15 part, dry Fructus Crataegi powder 6-16 part, artemia powder 12-20 part, dry Lumbricus powder 12-20 part, fish oil 10-20 part.
2. the immunostimulant of control tilapia branchiomycosis according to claim 1, it is characterized in that: the immunostimulant of described control tilapia branchiomycosis, be made up of each component of following masses number: Flos Lonicerae 36 parts, Rhizoma Acori Graminei 29 parts, Herba Pogostemonis 29 parts, Herba Setariae Viridis 27 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 26 parts, Herba Portulacae 24 parts, Herba Euphorbiae Helioscopiae 23 parts, Herba Abutili 24 parts, Fructus Tribuli 22 parts, Herba Violae 22 parts, Galla Chinensis 23 parts, Radix Alangii 20 parts, Radix Stephaniae Cepharanthae 20 parts, Herba Houttuyniae 20 parts, Fructus Ligustri Lucidi 20 parts, Herba Saginae Japonicae 18 parts, Herba Hedyotidis Diffusae 18 parts, Periostracum Cicadae 17 parts, conch meal 8 parts, Pericarpium Citri Reticulatae 15 parts, Rhizoma Cyperi 16 parts, Flos Daturae 14 parts, white vinegar 16 parts, 12 parts, Bulbus Allii, Mel 10 parts, dry Fructus Crataegi powder 10 parts, artemia powder 15 parts, dry Lumbricus powder 14 parts, 17 parts, fish oil.
3. the immunostimulant of control tilapia branchiomycosis according to claim 1, it is characterized in that: the immunostimulant of described control tilapia branchiomycosis, be made up of each component of following masses number: Flos Lonicerae 36 parts, Rhizoma Acori Graminei 28 parts, Herba Pogostemonis 28 parts, Herba Setariae Viridis 26 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 25 parts, Herba Portulacae 25 parts, Herba Euphorbiae Helioscopiae 24 parts, Herba Abutili 24 parts, Fructus Tribuli 20 parts, Herba Violae 21 parts, Galla Chinensis 22 parts, Radix Alangii 22 parts, Radix Stephaniae Cepharanthae 21 parts, Herba Houttuyniae 20 parts, Fructus Ligustri Lucidi 18 parts, Herba Saginae Japonicae 20 parts, Herba Hedyotidis Diffusae 20 parts, Periostracum Cicadae 18 parts, conch meal 7 parts, Pericarpium Citri Reticulatae 14 parts, Rhizoma Cyperi 15 parts, Flos Daturae 15 parts, white vinegar 15 parts, 12 parts, Bulbus Allii, Mel 12 parts, dry Fructus Crataegi powder 9 parts, artemia powder 16 parts, dry Lumbricus powder 16 parts, 15 parts, fish oil.
4. a preparation method for the immunostimulant of the control tilapia branchiomycosis according to any one of claim 1-3, is characterized in that: comprise the following steps:
(1) take Flos Lonicerae according to proportioning, add the purified water that parts by weight are Flos Lonicerae parts by weight 20 times, 4-6h is soaked in 70-80 DEG C of water-bath, filters to obtain infusion;
(2) Rhizoma Acori Graminei, Herba Pogostemonis, Herba Setariae Viridis, Herba Astragali Melilotoidis (Herba Astragali Sinici), Herba Portulacae, Herba Euphorbiae Helioscopiae, Herba Abutili, Fructus Tribuli, Herba Violae, Herba Houttuyniae, Herba Saginae Japonicae, Herba Hedyotidis Diffusae, Pericarpium Citri Reticulatae and Bulbus Allii is taken according to proportioning, gross weight is designated as 1 part, add 6 parts of purified water, 20-30 DEG C is soaked 6-9h, obtains soak;
(3) boil 2h after the soak that step (2) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 3 parts in medical material, continue to boil 1h after being heated to boiling, merge twice fried liquid and be extracting solution;
(4) take Galla Chinensis, Radix Alangii, Radix Stephaniae Cepharanthae, Fructus Ligustri Lucidi, Periostracum Cicadae, conch meal, Rhizoma Cyperi and Flos Daturae respectively according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(5) grounds travel that extracting solution step (3) obtained and step (4) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(6) take white vinegar, Mel, dry Fructus Crataegi powder, artemia powder, dry Lumbricus powder and fish oil according to proportioning, mix homogeneously with the infusion of step (1) gained, the extract of step (5) gained, obtain object.
5. a using method for the immunostimulant of the control tilapia branchiomycosis according to any one of claim 1-3, is characterized in that: throw something and feed after being mixed homogeneously with the weight ratio of 1:30-1:10 with commercially available normal feedstuff by the immunostimulant of control tilapia branchiomycosis of the present invention.
6. the using method of the immunostimulant of control tilapia branchiomycosis according to claim 5, is characterized in that: the immunostimulant of control tilapia branchiomycosis of the present invention and the weight ratio of commercially available normal feedstuff are 1:20.
7. the preparation method of the immunostimulant of control tilapia branchiomycosis according to claim 4, it is characterized in that: the preparation method of the Fructus Crataegi powder of described drying is: cleaned up by Fructus Crataegi, grind on ice after lyophilization to finish-drying in freezer dryer, after 50 mesh sieves, obtain dry Fructus Crataegi powder.
8. the preparation method of the immunostimulant of control tilapia branchiomycosis according to claim 4, it is characterized in that: the preparation method of the Lumbricus powder of described drying is: the Lumbricus lyophilization cleaned up is ground on ice to finish-drying, after 50 mesh sieves, obtain dry Lumbricus powder.
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