CN105558422A - Feed additive with functions of prevention and treatment of bacillary enteritis of plecoglossus altivelis and preparation method of feed additive - Google Patents
Feed additive with functions of prevention and treatment of bacillary enteritis of plecoglossus altivelis and preparation method of feed additive Download PDFInfo
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- CN105558422A CN105558422A CN201510971297.0A CN201510971297A CN105558422A CN 105558422 A CN105558422 A CN 105558422A CN 201510971297 A CN201510971297 A CN 201510971297A CN 105558422 A CN105558422 A CN 105558422A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
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Abstract
The invention belongs to the technical field of aquaculture, relates to a feed additive with functions of prevention and treatment of bacillary enteritis of plecoglossus altivelis and further relates to a preparation method of the feed additive with the functions of prevention and treatment of bacillary enteritis of the plecoglossus altivelis. The feed additive with the functions of prevention and treatment of bacillary enteritis of the plecoglossus altivelis is prepared from, by weight, 32-40 parts of east wall soil, 24-30 parts of Pericarpium Granati, 24-30 parts of Herba Hydrocotylis, 23-30 parts of Fructus Crataegi, 22-30 parts of Fructus Gleditsiae, 21-28 parts of Galla Chinensis, 19-27 parts of Radix Ophiopogonis, 19-27 parts of Radix Puerariae, 18-25 parts of Herba Asari, 18-26 parts of Radix Scutellariae, 17-25 parts of Poria Cocos, 6-14 parts of yeast powder, 8-17 parts of Plumula Nelumbinis, 8-15 parts of Pericarpium Citri Reticulatae, 4-5 parts of clam powder, 16-26 parts of Rhizoma Phragmitis and the like. The feed additive is effective in prevention and treatment of bacillary enteritis of the plecoglossus altivelis.
Description
Technical field
The invention belongs to technical field of aquaculture, particularly relate to a kind of feed addictive preventing and treating sweetfish bacterial enteritis disease; The invention still further relates to a kind of preparation method of feed addictive of above-mentioned control sweetfish bacterial enteritis disease.
Background technology
The most pressing issues that the sharp increase of population brings provide food for the mankind, and aquatic products are as a class high protein, low-fat high value food in food, and people increase severely to its demand nature and day.Sweetfish body is long and narrow and flat-sided, little and kiss point, and the large eye of mouth is little, and back of the body edge is grey black, both sides and belly are white, and tail bifurcated, without hard sour jujube, have a little adipose fin after dorsal fin, time fresh and alive, each fin is faint yellow, and there is the yellow color spot in a place top of abdomeinal fin, sweetfish kiss end is lower curved, and form elegant jessamine, respectively there is a projection lower neck front end, become significantly depression between two projections, lead up and down and respectively have the flat closely-pitched of a line width, can be movable, and be born on skin, vomer anodontia, tool tooth on jawbone and tongue.Whole body all has tiny cycloid scale except head, tool adipose fin, and body length can reach 30 cm, and sweetfish oppresses delicate greasiness, delicious and savory, and being first-class food fish, is famous and precious economic fish, have the good reputation of " kings of fresh-water fishes ".Sweetfish is because meat is tender, delicious, it is Japanese to be deeply subject to peculiar fragrance, Taiwan consumer likes, and has very high economic worth.In recent years, the some areas such as Fujian, Zhejiang and Hebei had started a burst of cultivation upsurge, and according to incompletely statistics, the sweetfish cultivation quantity that only Fujian, two places, Zhejiang are annual is more than 6000 tons.
Enteritis disease is the common disease of sweetfish, and this disease all can occur throughout the year.Sweetfish enteritis disease that the cause of disease has mainly eaten uncleanly food or satiety of ingesting, enteron aisle is swollen is full, excretion is obstructed, finally cause enteritis by bacteriological infection enteron aisle, its pathogen is Aeromonas punctata.Its cardinal symptom is: sick fish usually belly is expanded, and presents erythema, and anus redness is outstanding, and sometimes can extrude yellow mucus, fish body is dull, is parked in aquarium corner motionless, and body flesh does the tic of short time, does not take food, ight soil white; Cut fish belly open, visible hydroabdomen, the congested inflammation of intestines wall, the lighter only partial enteral occurs red, and time serious, full intestines are aubergine, without food in intestines, are filled with flaxen mucus and blood purulence.There is no the medicine of special for treating sweetfish enteritis disease at present, the common method for the treatment of of treatment sweetfish enteritis disease is thrown something and fed for the dipping bath or add in feed of splashing with conventional sterilization antiphlogistics such as orfloxacin, nitrofurazone, gentamicin or terramycin, its shortcoming is that result for the treatment of is not good, toxicity is larger, toxic residual in sweetfish body, not only environment easily polluted, affect the sustainable health development of culture fishery, and the sweetfish after edible healing also can affect the healthy of people.
Summary of the invention
An object of the present invention is to provide a kind of feed addictive preventing and treating sweetfish bacterial enteritis disease, and its raw material components is green pure natural material, and easy to use, has good prevention and therapy effect to sweetfish bacterial enteritis disease.
In order to solve the problems of the technologies described above, the present invention by the following technical solutions, a kind of feed addictive preventing and treating sweetfish bacterial enteritis disease, be made up of each component of following masses number: eastern wall soil 32-40 part, granatum 24-30 part, pennywort 24-30 part, hawthorn 23-30 part, Chinese honey locust 22-30 part, Chinese gall 21-28 part, the tuber of dwarf lilyturf 19-27 part, root of kudzu vine 19-27 part, root of Chinese wild ginger 18-25 part, root of large-flowered skullcap 18-26 part, Poria cocos 17-25 part, dusty yeast 6-14 part, lotus nut 8-17 part, dried orange peel 8-15 part, clam meal 4-5 part, reed rhizome 16-26 part, moutan bark 14-24 part, lophatherum gracile 12-20 part, Gorgon fruit 15-22 part, balsam pear 15-21 part, the membrane of a chicken's gizzard 6-15 part, bark of ash 14-21 part, Asiatic plantain 12-20 part, rhizoma atractylodis 11-18 part, radix pseudostellariae 12-18 part, smartweed 13-20 part, wartwort 12-19 part, cordate houttuynia 10-20 part, sealwort 9-18 part, earthworm powder 15-23 part, clam worm powder 10-20 part, winter honey 7-13 part, fish oil 8-16 part.
Preferably, the feed addictive of described control sweetfish bacterial enteritis disease, be made up of each component of following masses number: eastern 35 parts, wall soil, granatum 28 parts, pennywort 28 parts, hawthorn 27 parts, 25 parts, Chinese honey locust, 25 parts, Chinese gall, the tuber of dwarf lilyturf 23 parts, the root of kudzu vine 22 parts, the root of Chinese wild ginger 22 parts, the root of large-flowered skullcap 21 parts, 20 parts, Poria cocos, dusty yeast 10 parts, lotus nut 12 parts, dried orange peel 13 parts, clam meal 4 parts, reed rhizome 20 parts, moutan bark 18 parts, lophatherum gracile 16 parts, Gorgon fruit 18 parts, 18 parts, balsam pear, the membrane of a chicken's gizzard 9 parts, the bark of ash 18 parts, Asiatic plantain 16 parts, rhizoma atractylodis 15 parts, radix pseudostellariae 15 parts, smartweed 17 parts, wartwort 16 parts, cordate houttuynia 15 parts, sealwort 14 parts, earthworm powder 18 parts, 15 parts, clam worm powder, winter honey 10 parts, 12 parts, fish oil.
Preferably, the feed addictive of described control sweetfish bacterial enteritis disease, be made up of each component of following masses number: eastern 36 parts, wall soil, granatum 26 parts, pennywort 27 parts, hawthorn 26 parts, 26 parts, Chinese honey locust, 24 parts, Chinese gall, the tuber of dwarf lilyturf 24 parts, the root of kudzu vine 24 parts, the root of Chinese wild ginger 21 parts, the root of large-flowered skullcap 23 parts, 22 parts, Poria cocos, dusty yeast 9 parts, lotus nut 14 parts, dried orange peel 12 parts, clam meal 5 parts, reed rhizome 21 parts, moutan bark 20 parts, lophatherum gracile 15 parts, Gorgon fruit 19 parts, 18 parts, balsam pear, the membrane of a chicken's gizzard 10 parts, the bark of ash 17 parts, Asiatic plantain 15 parts, rhizoma atractylodis 14 parts, radix pseudostellariae 15 parts, smartweed 16 parts, wartwort 15 parts, cordate houttuynia 16 parts, sealwort 13 parts, earthworm powder 20 parts, 14 parts, clam worm powder, winter honey 9 parts, 14 parts, fish oil.
Each component effect of the present invention is resolved as follows:
East wall soil is sweet, warm, nontoxic, can toxin expelling, detoxify, Huang of dispelling, remove edema, anti-inflammation and sterilization, calmness releives.Granatum is the dry pericarp of Punicaceae plant pomegranate, has relieving diarrhea with astringents, hemostasis, effect of expelling parasite, and be usually used in rushing down for a long time, protracted dysentery, has blood in stool, prolapse of the anus, uterine bleeding, under band, and abdominal pain due to enterositosis.Pennywort is a kind of Umbelliferae pennywort subordinate, belongs to the wild caraway of the green type of nuisanceless health care, all herbal medicine, heat-clearing, diuresis, detumescence, removing toxic substances, subcutaneous ulcer of harnessing the Yellow River, dysentery, order screen, larynx are swollen, ulcer furunculosis, falls and beat bruise; In vitro test has comparatively high inhibition effect to staphylococcus aureus, and to proteus, shigella flexneri, typhoid bacillus also have the inhibitory action of not degree; Plague model test result, it is 54% that mouse mould suppresses, and by row antimalarial the selection result during pennywort herb 100g/kg, inhibiting rate is 54%.Hawthorn to have another name called in mountain fruit, large-fruited Chinese hawthorn, can bactericidal antiphlogistic, and has the effects such as reducing blood lipid, blood pressure, cardiac stimulant, arrhythmia, is also the good medicine of spleen benefiting and stimulating the appetite, relieving dyspepsia, phlegm reduction of blood circulation promoting simultaneously.Chinese honey locust is containing triterpenoid saponin (gledinin, gleditschiasaponin), tannin, ceryl alcohol, nonacosane, stigmasterol, sitosterol etc., the function of have eliminating the phlegm, having one's ideas straightened out, saponin(e element contained in Chinese honey locust is triterpene class and compound sugar, there are anti-inflammatory, antiulcer, disease-resistant change effect, also have anticancer and improve the effects such as AIDS immunity.Chinese gall is cold in nature, taste acid, puckery, and having astringes the lung falls the effects such as fire, relieving diarrhea with astringents, arrest sweating hemostasis, hygroscopic sore, cures mainly chronic cough of deficiency lung, rushes down protracted dysentery for a long time, the disease such as hemorrhoid of having blood in stool blood, carbuncle sore tumefacting virus, skin moisture fester.Can enter stomach the tuber of dwarf lilyturf with nourishing the stomach liquid, stimulating appetite, spleen is loose is skillful in lung, Dingchuan relieve coughing to help more to enter spleen.The root of kudzu vine has relieving muscles to expel heat, promoting eruption, promotes the production of body fluid to quench thirst, and rises the merit of positive antidiarrheal, and be usually used in illness that has not attacked the vital organs of the human body heating, stiff nape and back, measles without adequate eruption, pyreticosis is thirsty, and the deficiency of Yin is quenched one's thirst, and heat is purged heat dysentery, splenasthenic diarrhea.The root of Chinese wild ginger can induce sweat loose cold, and wind-expelling pain-stopping is sensible, warming lung to reduce watery phlegm.Root of large-flowered skullcap bitter, cold in nature, there are heat-clearing and damp-drying drug, purging intense heat and detonicating, hemostasis, the effect such as antiabortive, cure mainly warm heat disease, the infection of the upper respiratory tract, cough with lung heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, hot eyes, fetal irritability, hypertension, carbuncle swell the diseases such as furuncle sore, the clinical antibiotic property of the root of large-flowered skullcap is better than the coptis, and does not develop immunity to drugs.Poria cocos is used for oedema oliguria, and phlegm and retained fluid is dizzy throbs with fear, and insufficiency of the spleen food is few, has loose bowels in loose stool, confused and worried, palpitation with fear insomnia.Dusty yeast is rich in various trace elements, is the natural flavouring that a kind of nutritive value is very high, and dusty yeast can also promote to digest and assimilate in addition.Lotus nut and lotus nut, have clearing away the heart-fire, flat irascibility, hemostasis, effect of controlling nocturnal emission with astringent drugs.The promoting the circulation of qi of dried orange peel energy and in, stomach Qi rising, n and V can be controlled, and can bactericidal.Clam meal nature and flavor are salty, cold, and energy heat-clearing, dampness removing, reduces phlegm, soft heavily fortified point, makes the fishy smell that can remove medicine altogether, heighten the effect of a treatment with medicine.Reed rhizome taste is sweet, cold in nature, returns lung channel, stomach warp, drenches puckery pain for pyreticosis polydipsia, gastropyretic vomiting, cough with lung heat, lung carbuncle pyemesis, heat.Moutan bark is bitter, pungent, is slightly cold, the thoughts of returning home, liver, kidney channel, has clearing heat and cooling blood, activates blood circulation and disperses blood clots, the effect such as reducing the asthenic fever.Lophatherum gracile is grass family, and lophatherum gracile belongs to herbaceos perennial, has refrigerant, antipyretic, diuresis and the effect of hastening parturition; Cure mainly: in the heart disease heat, cough with dyspnea, haematemesis, hot malicious wind, only quench one's thirst, press borax poison, dissolving phlegm, control fanatical unhappiness, apoplectic aphasia in silence, pain head wind, only palpitation with fear, pestilence fan be vexed, kill small worm, heat extraction delays spleen.Gorgon fruit is used for emission and spermatorrhea, and enuresis frequent micturition is insufficiency of the spleenly rushed down for a long time, and gonorrhoea, under band.Balsam pear has clearing heat and detoxicating effect.The membrane of a chicken's gizzard returns spleen, stomach, small intestine, urinary bladder channel, can stomach strengthening and digestion promoting, arresting seminal emission, and treating stranguria fossil, can be used for the treatment of dyspepsia and not disappear, vomiting dysentery, infantile malnutrition, the enuresis, seminal emission, the puckery pain of urolithiasis, hypochondriac pain that courage is swollen.Bark of ash bitter, puckery, cold in nature, cure mainly heat-clearing and damp-drying drug, inducing astrigency stops dysentery, only band, improving eyesight, rush down dysentery, leukorrhea with reddish discharge, red eye, swell pain, the raw ocular of order for hot poison.Asiatic plantain, Chinese medicine name, heat-clearing, diuresis, eliminates the phlegm, cool blood, removing toxic substances, and for curing mainly oedema oliguria, heat drenches puckery pain, and summer-heat and damp rushes down dysentery, phlegm-heat cough, hematemesis and epistaxis, carbuncle sore tumefacting virus.Rhizoma atractylodis, taste is pungent, bitter, warm in nature, returns spleen, stomach, Liver Channel, and for damp retention in middle-jiao, abdominal fullness and distention, has loose bowels, oedema, and beriberi impotence is knitted, and arthralgia due to wind-dampness, anemofrigid cold, yctalopia, eyes dusk is puckery.Radix pseudostellariae has and replenishes qi to invigorate the spleen, and promoting production of body fluid and nourishing the lung effect, is usually used in insufficiency of the spleen body tired, poor appetite, weak after being ill, and deficiency of both vital energy and Yin, spontaneous perspiration day is thirsty, dryness of the lung dry cough.Smartweed is invigorated blood circulation, pain relieving, disappear long-pending, diuresis effect.Wartwort taste is pungent, bitter, cold nature, line water detumescence, preventing phlegm from forming and stopping coughing, and removing toxic substances desinsection, cures mainly edematous asthma, and phlegm and retained fluid is breathed with cough malaria, bacillary dysentery, scrofula, Tuberculous fistula, osteomyelitis.Cordate houttuynia has clearing heat and detoxicating, the carbuncle that disappears apocenosis, the effect of inducing diuresis for treating strangurtia.Sealwort herb can hyoscine, has the effect of removing toxic substances of bringing down a fever, and fresh leaf rubs juice, and to be coated with dermatitis rhus effective, and can control dermatitis rhus, the favus of the scalp, carbuncle swells, scrofula, carious tooth, and children's's breast is long-pending, internal traumatic injury.Also containing abundant vitamin D and the mineral matter element such as calcium and phosphorus in earthworm body, containing the multiple medicinal ingredient such as earthworm element, earthworm toxolysin, xanthine, antihistaminicum and Cobastab in earthworm body, and earthworm delicious flavour, to sweetfish, there is food calling effect.Clam worm has anti-oxidant, antibacterial, and radioresistance is antiviral, antifatigue, and anti-cancer, immunity moderation, the effect delayed senility, and delicious meat, be one of important bait of fish, have food calling effect to most fish.Winter honey not only can strengthen the immunity of body, and can be in harmonious proportion feed mouthfeel.Fish oil can be sweetfish makeup energy, also has food calling effect to sweetfish.
Another object of the present invention is to provide a kind of preparation method of feed addictive of above-mentioned control sweetfish bacterial enteritis disease, and the preparation method of the feed addictive of this control sweetfish bacterial enteritis disease comprises the following steps:
(1) take eastern wall soil and clam meal according to proportioning, eastern wall soil is mixed with clam meal, at 120 ~ 160 DEG C of frying 5-8 minute, puts into pulverizer after frying completes and pulverize and cross 100 mesh sieves, obtain comminuted powder;
(2) by mass concentration be 50% alcohol add in Chinese medicine extracting tank, alcohol consumption is 10 times of the comminuted powder weight of step (1) gained, open and stir, the comminuted powder of step (1) gained is added in Chinese medicine extracting tank, temperature of charge in Chinese medicine extracting tank is warming up to 77 DEG C of constant temperature and extracts 30min, 100 orders cross leaching filtrate, and filter residue returns in Chinese medicine extracting tank and repeats to extract once, 100 orders cross leaching filtrate, merge twice gained filtrate;
(3) granatum, pennywort, hawthorn, Chinese honey locust, Chinese gall, the tuber of dwarf lilyturf, the root of kudzu vine, the root of Chinese wild ginger, the root of large-flowered skullcap, Poria cocos, dried orange peel, reed rhizome, moutan bark and lophatherum gracile is taken according to proportioning, gross weight is designated as 1 part, add 6 parts of purified water, 70-80 DEG C is soaked 6-9h, obtains soak;
(4) boil 2h after the soak that step (3) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 4 parts in medicinal material, continue to boil 1h after being heated to boiling, merge twice fried liquid and be extract;
(5) take lotus nut, Gorgon fruit, balsam pear, the membrane of a chicken's gizzard, the bark of ash, Asiatic plantain, rhizoma atractylodis, radix pseudostellariae, smartweed, wartwort, cordate houttuynia and sealwort respectively according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(6) grounds travel that extract step (4) obtained and step (5) obtain is positioned in the extraction kettle in supercritical extract instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(7) take dusty yeast, earthworm powder, clam worm powder, winter honey and fish oil respectively according to proportioning, mix with the filtrate of step (2) gained, the extract of step (6) gained, obtain object.
The preparation method of described earthworm powder is: the earthworm freeze drying cleaned up ground on ice to finish-drying, after 50 mesh sieves, obtain earthworm powder.Cryodesiccated advantage to make active material inactivation contained in earthworm in dry run, produces heat, to keep temperature, protective tissue when the advantage of grinding is and reduces grinding on ice because constantly stirring.
The preparation method of described clam worm powder is: the clam worm freeze drying cleaned up ground on ice to finish-drying, after 50 mesh sieves, obtains clam worm powder.Cryodesiccated advantage to make active material inactivation contained in clam worm in dry run, produces heat, to keep temperature, protective tissue when the advantage of grinding is and reduces grinding on ice because constantly stirring.
Usage and dosage: throw something and feed after the feed addictive of control sweetfish bacterial enteritis disease of the present invention is mixed with the weight ratio of 1:35-1:10 with commercially available basal feed, preferably, the feed addictive of control sweetfish enteritis disease of the present invention and the weight ratio of commercially available basal feed are 1:20.
In preparation method, with the effect that clam meal frying east wall soil can strengthen eastern wall soil anti-inflammation and sterilization, calmness is releived; By purified to granatum, pennywort, hawthorn, Chinese honey locust, Chinese gall, the tuber of dwarf lilyturf, the root of kudzu vine, the root of Chinese wild ginger, the root of large-flowered skullcap, Poria cocos, dried orange peel, reed rhizome, moutan bark and lophatherum gracile water soaking a period of time, medicinal material deliquescing, cell expansion, active ingredient is easily fried, and medicinal material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medicinal material surface, protein coagulating, the capillary channel on blocking medicinal material surface, makes the moisture when decocting enter not go, active ingredient is not easily fried, affects curative effect of medication; Decoction can change the irritation effect of medicine, relatively mildly acts on sweetfish, promotes that sweetfish is to the absorption of medicine; By lotus nut, Gorgon fruit, balsam pear, the membrane of a chicken's gizzard, the bark of ash, Asiatic plantain, rhizoma atractylodis, radix pseudostellariae, smartweed, wartwort, cordate houttuynia with sealwort grinding can make the starch in medicinal material, protein separates out more fully at subsequent step; Supercritical extract step can retain the active ingredient of each component in the present invention to greatest extent.
Because the fish oil contained in feed addictive of the present invention for sweetfish makeup energy and can not only have food calling effect, earthworm and clam worm delicious flavour, winter honey can improve the overall mouthfeel of feed, earthworm powder, clam worm powder, winter honey and fish oil have collaborative food calling effect to sweetfish, therefore the feed addictive of control sweetfish enteritis disease of the present invention can improve the mouthfeel of basal feed, stimulate the taste bud of sweetfish, induction sweetfish ingests and with the addition of the basal feed of feed addictive of the present invention, therefore sweetfish immunity and resistance can be strengthened in feed addictive of the present invention, the composition pathogen of enteritis disease to remarkable bactericidal and bacteriostatic effect can enter in sweetfish body, and play it and act on accordingly.
The feed addictive of control sweetfish bacterial enteritis disease of the present invention is the nosogenesis according to enteritis disease, the suitable component of selection, and is organically combined, and has significant cooperative synergism effect, plays the function of each component to greatest extent.The component of the feed addictive of control sweetfish bacterial enteritis disease of the present invention is environmental protection raw material, nontoxic to environment, not containing chemicals or antibiotic, be conducive to the sustainable health development of sweetfish cultivation industry, the healthy of people can not be affected after edible sweetfish of having cured, and feed addictive of the present invention is good to the curative effect of sweetfish enteritis disease, cure rate is high, make the survival rate of ill sweetfish to more than 90%, after medication 1-3 days, sweetfish starts progressively to recover to ingest, and ingestion frequency improves.In addition, the present invention uses safer, little to sweetfish damage, and after using the feed addictive for the treatment of sweetfish enteritis disease of the present invention, sweetfish Health restoration is fast, medication 2-5 days, and sweetfish can recover normal activity, and idle fund is strong and vigorous, ingests completely normal.In addition, the feed addictive of control sweetfish bacterial enteritis disease of the present invention effectively can also prevent the generation of sweetfish enteritis disease.
Compared with prior art, the invention has the beneficial effects as follows:
(1), in each component of the present invention, Dong Bitu is sweet, warm, nontoxic, can toxin expelling, detoxify, Huang of dispelling, remove edema, anti-inflammation and sterilization, calmness releives.Granatum is the dry pericarp of Punicaceae plant pomegranate, has relieving diarrhea with astringents, hemostasis, effect of expelling parasite, and be usually used in rushing down for a long time, protracted dysentery, has blood in stool, prolapse of the anus, uterine bleeding, under band, and abdominal pain due to enterositosis.Pennywort is a kind of Umbelliferae pennywort subordinate, belongs to the wild caraway of the green type of nuisanceless health care, all herbal medicine, heat-clearing, diuresis, detumescence, removing toxic substances, subcutaneous ulcer of harnessing the Yellow River, dysentery, order screen, larynx are swollen, ulcer furunculosis, falls and beat bruise; In vitro test has comparatively high inhibition effect to staphylococcus aureus, and to proteus, shigella flexneri, typhoid bacillus also have the inhibitory action of not degree; Plague model test result, it is 54% that mouse mould suppresses, and by row antimalarial the selection result during pennywort herb 100g/kg, inhibiting rate is 54%.Hawthorn to have another name called in mountain fruit, large-fruited Chinese hawthorn, can bactericidal antiphlogistic, and has the effects such as reducing blood lipid, blood pressure, cardiac stimulant, arrhythmia, is also the good medicine of spleen benefiting and stimulating the appetite, relieving dyspepsia, phlegm reduction of blood circulation promoting simultaneously.Chinese honey locust is containing triterpenoid saponin (gledinin, gleditschiasaponin), tannin, ceryl alcohol, nonacosane, stigmasterol, sitosterol etc., the function of have eliminating the phlegm, having one's ideas straightened out, saponin(e element contained in Chinese honey locust is triterpene class and compound sugar, there are anti-inflammatory, antiulcer, disease-resistant change effect, also have anticancer and improve the effects such as AIDS immunity.Chinese gall is cold in nature, taste acid, puckery, and having astringes the lung falls the effects such as fire, relieving diarrhea with astringents, arrest sweating hemostasis, hygroscopic sore, cures mainly chronic cough of deficiency lung, rushes down protracted dysentery for a long time, the disease such as hemorrhoid of having blood in stool blood, carbuncle sore tumefacting virus, skin moisture fester.Can enter stomach the tuber of dwarf lilyturf with nourishing the stomach liquid, stimulating appetite, spleen is loose is skillful in lung, Dingchuan relieve coughing to help more to enter spleen.The root of kudzu vine has relieving muscles to expel heat, promoting eruption, promotes the production of body fluid to quench thirst, and rises the merit of positive antidiarrheal, and be usually used in illness that has not attacked the vital organs of the human body heating, stiff nape and back, measles without adequate eruption, pyreticosis is thirsty, and the deficiency of Yin is quenched one's thirst, and heat is purged heat dysentery, splenasthenic diarrhea.The root of Chinese wild ginger can induce sweat loose cold, and wind-expelling pain-stopping is sensible, warming lung to reduce watery phlegm.Root of large-flowered skullcap bitter, cold in nature, there are heat-clearing and damp-drying drug, purging intense heat and detonicating, hemostasis, the effect such as antiabortive, cure mainly warm heat disease, the infection of the upper respiratory tract, cough with lung heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, hot eyes, fetal irritability, hypertension, carbuncle swell the diseases such as furuncle sore, the clinical antibiotic property of the root of large-flowered skullcap is better than the coptis, and does not develop immunity to drugs.Poria cocos is used for oedema oliguria, and phlegm and retained fluid is dizzy throbs with fear, and insufficiency of the spleen food is few, has loose bowels in loose stool, confused and worried, palpitation with fear insomnia.Dusty yeast is rich in various trace elements, is the natural flavouring that a kind of nutritive value is very high, and dusty yeast can also promote to digest and assimilate in addition.Lotus nut and lotus nut, have clearing away the heart-fire, flat irascibility, hemostasis, effect of controlling nocturnal emission with astringent drugs.The promoting the circulation of qi of dried orange peel energy and in, stomach Qi rising, n and V can be controlled, and can bactericidal.Clam meal nature and flavor are salty, cold, and energy heat-clearing, dampness removing, reduces phlegm, soft heavily fortified point, makes the fishy smell that can remove medicine altogether, heighten the effect of a treatment with medicine.Reed rhizome taste is sweet, cold in nature, returns lung channel, stomach warp, drenches puckery pain for pyreticosis polydipsia, gastropyretic vomiting, cough with lung heat, lung carbuncle pyemesis, heat.Moutan bark is bitter, pungent, is slightly cold, the thoughts of returning home, liver, kidney channel, has clearing heat and cooling blood, activates blood circulation and disperses blood clots, the effect such as reducing the asthenic fever.Lophatherum gracile is grass family, and lophatherum gracile belongs to herbaceos perennial, has refrigerant, antipyretic, diuresis and the effect of hastening parturition; Cure mainly: in the heart disease heat, cough with dyspnea, haematemesis, hot malicious wind, only quench one's thirst, press borax poison, dissolving phlegm, control fanatical unhappiness, apoplectic aphasia in silence, pain head wind, only palpitation with fear, pestilence fan be vexed, kill small worm, heat extraction delays spleen.Gorgon fruit is used for emission and spermatorrhea, and enuresis frequent micturition is insufficiency of the spleenly rushed down for a long time, and gonorrhoea, under band.Balsam pear has clearing heat and detoxicating effect.The membrane of a chicken's gizzard returns spleen, stomach, small intestine, urinary bladder channel, can stomach strengthening and digestion promoting, arresting seminal emission, and treating stranguria fossil, can be used for the treatment of dyspepsia and not disappear, vomiting dysentery, infantile malnutrition, the enuresis, seminal emission, the puckery pain of urolithiasis, hypochondriac pain that courage is swollen.Bark of ash bitter, puckery, cold in nature, cure mainly heat-clearing and damp-drying drug, inducing astrigency stops dysentery, only band, improving eyesight, rush down dysentery, leukorrhea with reddish discharge, red eye, swell pain, the raw ocular of order for hot poison.Asiatic plantain, Chinese medicine name, heat-clearing, diuresis, eliminates the phlegm, cool blood, removing toxic substances, and for curing mainly oedema oliguria, heat drenches puckery pain, and summer-heat and damp rushes down dysentery, phlegm-heat cough, hematemesis and epistaxis, carbuncle sore tumefacting virus.Rhizoma atractylodis, taste is pungent, bitter, warm in nature, returns spleen, stomach, Liver Channel, and for damp retention in middle-jiao, abdominal fullness and distention, has loose bowels, oedema, and beriberi impotence is knitted, and arthralgia due to wind-dampness, anemofrigid cold, yctalopia, eyes dusk is puckery.Radix pseudostellariae has and replenishes qi to invigorate the spleen, and promoting production of body fluid and nourishing the lung effect, is usually used in insufficiency of the spleen body tired, poor appetite, weak after being ill, and deficiency of both vital energy and Yin, spontaneous perspiration day is thirsty, dryness of the lung dry cough.Smartweed is invigorated blood circulation, pain relieving, disappear long-pending, diuresis effect.Wartwort taste is pungent, bitter, cold nature, line water detumescence, preventing phlegm from forming and stopping coughing, and removing toxic substances desinsection, cures mainly edematous asthma, and phlegm and retained fluid is breathed with cough malaria, bacillary dysentery, scrofula, Tuberculous fistula, osteomyelitis.Cordate houttuynia has clearing heat and detoxicating, the carbuncle that disappears apocenosis, the effect of inducing diuresis for treating strangurtia.Sealwort herb can hyoscine, has the effect of removing toxic substances of bringing down a fever, and fresh leaf rubs juice, and to be coated with dermatitis rhus effective, and can control dermatitis rhus, the favus of the scalp, carbuncle swells, scrofula, carious tooth, and children's's breast is long-pending, internal traumatic injury.Also containing abundant vitamin D and the mineral matter element such as calcium and phosphorus in earthworm body, containing the multiple medicinal ingredient such as earthworm element, earthworm toxolysin, xanthine, antihistaminicum and Cobastab in earthworm body, and earthworm delicious flavour, to sweetfish, there is food calling effect.Clam worm has anti-oxidant, antibacterial, and radioresistance is antiviral, antifatigue, and anti-cancer, immunity moderation, the effect delayed senility, and delicious meat, be one of important bait of fish, have food calling effect to most fish.Winter honey not only can strengthen the immunity of body, and can be in harmonious proportion feed mouthfeel.Fish oil can be sweetfish makeup energy, also has food calling effect to sweetfish.
(2) fish oil owing to containing in feed addictive of the present invention for sweetfish makeup energy and can not only have food calling effect, earthworm and clam worm delicious flavour, winter honey can improve the overall mouthfeel of feed, earthworm powder, clam worm powder, winter honey and fish oil have collaborative food calling effect to sweetfish, therefore the feed addictive of control sweetfish enteritis disease of the present invention can improve the mouthfeel of basal feed, stimulate the taste bud of sweetfish, induction sweetfish ingests and with the addition of the basal feed of feed addictive of the present invention, therefore sweetfish immunity and resistance can be strengthened in feed addictive of the present invention, the composition pathogen of enteritis disease to remarkable bactericidal and bacteriostatic effect can enter in sweetfish body, and play it and act on accordingly.
(3) pathogen of feed addictive to sweetfish enteritis disease of control sweetfish bacterial enteritis disease of the present invention has good inhibition.
(4) component of the feed addictive of control sweetfish bacterial enteritis disease of the present invention is environmental protection raw material, nontoxic to environment, without chemicals or antibiotic, the healthy of people can not be affected after edible sweetfish of having cured, and the feed addictive of control sweetfish bacterial enteritis disease of the present invention is very little to sweetfish damage, after using the feed addictive of control sweetfish bacterial enteritis disease of the present invention, sweetfish Health restoration is fast.In addition, the feed addictive of control sweetfish bacterial enteritis disease of the present invention is easy to use, will not pull rear medicine-feeding out by sick fish, decrease the injury to sick fish in operating process.
(5) in preparation method, with the effect that clam meal frying can strengthen eastern wall soil anti-inflammation and sterilization, calmness is releived; By purified to granatum, pennywort, hawthorn, Chinese honey locust, Chinese gall, the tuber of dwarf lilyturf, the root of kudzu vine, the root of Chinese wild ginger, the root of large-flowered skullcap, Poria cocos, dried orange peel, reed rhizome, moutan bark and lophatherum gracile water soaking a period of time, medicinal material deliquescing, cell expansion, active ingredient is easily fried, and medicinal material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medicinal material surface, protein coagulating, the capillary channel on blocking medicinal material surface, makes the moisture when decocting enter not go, active ingredient is not easily fried, affects curative effect of medication; Decoction can change the irritation effect of medicine, relatively mildly acts on sweetfish, promotes that sweetfish is to the absorption of medicine; By lotus nut, Gorgon fruit, balsam pear, the membrane of a chicken's gizzard, the bark of ash, Asiatic plantain, rhizoma atractylodis, radix pseudostellariae, smartweed, wartwort, cordate houttuynia with sealwort grinding can make the starch in medicinal material, protein separates out more fully at subsequent step; Supercritical extract step can retain the active ingredient of each component in the present invention to greatest extent.
Detailed description of the invention
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.
Embodiment 1
A kind of feed addictive preventing and treating sweetfish bacterial enteritis disease, be made up of each component of following masses number: eastern 38 parts, wall soil, granatum 30 parts, pennywort 30 parts, hawthorn 28 parts, 28 parts, Chinese honey locust, 26 parts, Chinese gall, the tuber of dwarf lilyturf 27 parts, the root of kudzu vine 26 parts, the root of Chinese wild ginger 25 parts, the root of large-flowered skullcap 26 parts, 25 parts, Poria cocos, dusty yeast 14 parts, lotus nut 17 parts, dried orange peel 14 parts, clam meal 5 parts, reed rhizome 26 parts, moutan bark 24 parts, lophatherum gracile 20 parts, Gorgon fruit 22 parts, 20 parts, balsam pear, the membrane of a chicken's gizzard 15 parts, the bark of ash 21 parts, Asiatic plantain 20 parts, rhizoma atractylodis 18 parts, radix pseudostellariae 18 parts, smartweed 18 parts, wartwort 19 parts, cordate houttuynia 20 parts, sealwort 18 parts, earthworm powder 22 parts, 20 parts, clam worm powder, winter honey 12 parts, 16 parts, fish oil.
The preparation method of the feed addictive of above-mentioned control sweetfish bacterial enteritis disease comprises the following steps:
(1) take eastern wall soil and clam meal according to proportioning, eastern wall soil is mixed with clam meal, at 120 ~ 160 DEG C of frying 5-8 minute, puts into pulverizer after frying completes and pulverize and cross 100 mesh sieves, obtain comminuted powder;
(2) by mass concentration be 50% alcohol add in Chinese medicine extracting tank, alcohol consumption is 10 times of the comminuted powder weight of step (1) gained, open and stir, the comminuted powder of step (1) gained is added in Chinese medicine extracting tank, temperature of charge in Chinese medicine extracting tank is warming up to 77 DEG C of constant temperature and extracts 30min, 100 orders cross leaching filtrate, and filter residue returns in Chinese medicine extracting tank and repeats to extract once, 100 orders cross leaching filtrate, merge twice gained filtrate;
(3) granatum, pennywort, hawthorn, Chinese honey locust, Chinese gall, the tuber of dwarf lilyturf, the root of kudzu vine, the root of Chinese wild ginger, the root of large-flowered skullcap, Poria cocos, dried orange peel, reed rhizome, moutan bark and lophatherum gracile is taken according to proportioning, gross weight is designated as 1 part, add 6 parts of purified water, 70-80 DEG C is soaked 6-9h, obtains soak;
(4) boil 2h after the soak that step (3) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 4 parts in medicinal material, continue to boil 1h after being heated to boiling, merge twice fried liquid and be extract;
(5) take lotus nut, Gorgon fruit, balsam pear, the membrane of a chicken's gizzard, the bark of ash, Asiatic plantain, rhizoma atractylodis, radix pseudostellariae, smartweed, wartwort, cordate houttuynia and sealwort respectively according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(6) grounds travel that extract step (4) obtained and step (5) obtain is positioned in the extraction kettle in supercritical extract instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(7) take dusty yeast, earthworm powder, clam worm powder, winter honey and fish oil respectively according to proportioning, mix with the filtrate of step (2) gained, the extract of step (6) gained, obtain object.
The preparation method of described earthworm powder is: the earthworm freeze drying cleaned up ground on ice to finish-drying, after 50 mesh sieves, obtain earthworm powder.
The preparation method of described clam worm powder is: the clam worm freeze drying cleaned up ground on ice to finish-drying, after 50 mesh sieves, obtains clam worm powder.
Embodiment 2
A kind of feed addictive preventing and treating sweetfish bacterial enteritis disease, be made up of each component of following masses number: eastern 40 parts, wall soil, granatum 28 parts, pennywort 28 parts, hawthorn 30 parts, 30 parts, Chinese honey locust, 28 parts, Chinese gall, the tuber of dwarf lilyturf 25 parts, the root of kudzu vine 27 parts, the root of Chinese wild ginger 25 parts, the root of large-flowered skullcap 25 parts, 24 parts, Poria cocos, dusty yeast 14 parts, lotus nut 15 parts, dried orange peel 15 parts, clam meal 5 parts, reed rhizome 25 parts, moutan bark 22 parts, lophatherum gracile 18 parts, Gorgon fruit 20 parts, 21 parts, balsam pear, the membrane of a chicken's gizzard 15 parts, the bark of ash 21 parts, Asiatic plantain 18 parts, rhizoma atractylodis 16 parts, radix pseudostellariae 18 parts, smartweed 20 parts, wartwort 18 parts, cordate houttuynia 17 parts, sealwort 16 parts, earthworm powder 23 parts, 18 parts, clam worm powder, winter honey 13 parts, 14 parts, fish oil.
The preparation method of the feed addictive of the control sweetfish bacterial enteritis disease of the present embodiment is with embodiment 1.
Embodiment 3
A kind of feed addictive preventing and treating sweetfish bacterial enteritis disease, be made up of each component of following masses number: eastern 32 parts, wall soil, granatum 24 parts, pennywort 25 parts, hawthorn 25 parts, 22 parts, Chinese honey locust, 21 parts, Chinese gall, the tuber of dwarf lilyturf 19 parts, the root of kudzu vine 20 parts, the root of Chinese wild ginger 18 parts, the root of large-flowered skullcap 18 parts, 17 parts, Poria cocos, dusty yeast 6 parts, lotus nut 10 parts, dried orange peel 8 parts, clam meal 4 parts, reed rhizome 16 parts, moutan bark 14 parts, lophatherum gracile 12 parts, Gorgon fruit 15 parts, 15 parts, balsam pear, the membrane of a chicken's gizzard 8 parts, the bark of ash 14 parts, Asiatic plantain 14 parts, rhizoma atractylodis 11 parts, radix pseudostellariae 12 parts, smartweed 13 parts, wartwort 12 parts, cordate houttuynia 10 parts, sealwort 10 parts, earthworm powder 15 parts, 10 parts, clam worm powder, winter honey 8 parts, 10 parts, fish oil.
The preparation method of the feed addictive of the control sweetfish bacterial enteritis disease of the present embodiment is with embodiment 1.
Embodiment 4
A kind of feed addictive preventing and treating sweetfish bacterial enteritis disease, be made up of each component of following masses number: eastern 34 parts, wall soil, granatum 26 parts, pennywort 24 parts, hawthorn 23 parts, 24 parts, Chinese honey locust, 24 parts, Chinese gall, the tuber of dwarf lilyturf 21 parts, the root of kudzu vine 19 parts, the root of Chinese wild ginger 20 parts, the root of large-flowered skullcap 20 parts, 17 parts, Poria cocos, dusty yeast 8 parts, lotus nut 8 parts, dried orange peel 8 parts, clam meal 4 parts, reed rhizome 18 parts, moutan bark 16 parts, lophatherum gracile 14 parts, Gorgon fruit 16 parts, 16 parts, balsam pear, the membrane of a chicken's gizzard 6 parts, the bark of ash 15 parts, Asiatic plantain 12 parts, rhizoma atractylodis 12 parts, radix pseudostellariae 12 parts, smartweed 13 parts, wartwort 13 parts, cordate houttuynia 12 parts, sealwort 9 parts, earthworm powder 16 parts, 12 parts, clam worm powder, winter honey 7 parts, 8 parts, fish oil.
The preparation method of the feed addictive of the control sweetfish bacterial enteritis disease of the present embodiment is with embodiment 1.
Embodiment 5
A kind of feed addictive preventing and treating sweetfish bacterial enteritis disease, be made up of each component of following masses number: eastern 35 parts, wall soil, granatum 28 parts, pennywort 28 parts, hawthorn 27 parts, 25 parts, Chinese honey locust, 25 parts, Chinese gall, the tuber of dwarf lilyturf 23 parts, the root of kudzu vine 22 parts, the root of Chinese wild ginger 22 parts, the root of large-flowered skullcap 21 parts, 20 parts, Poria cocos, dusty yeast 10 parts, lotus nut 12 parts, dried orange peel 13 parts, clam meal 4 parts, reed rhizome 20 parts, moutan bark 18 parts, lophatherum gracile 16 parts, Gorgon fruit 18 parts, 18 parts, balsam pear, the membrane of a chicken's gizzard 9 parts, the bark of ash 18 parts, Asiatic plantain 16 parts, rhizoma atractylodis 15 parts, radix pseudostellariae 15 parts, smartweed 17 parts, wartwort 16 parts, cordate houttuynia 15 parts, sealwort 14 parts, earthworm powder 18 parts, 15 parts, clam worm powder, winter honey 10 parts, 12 parts, fish oil.
The preparation method of the feed addictive of the control sweetfish bacterial enteritis disease of the present embodiment is with embodiment 1.
Embodiment 6
A kind of feed addictive preventing and treating sweetfish bacterial enteritis disease, be made up of each component of following masses number: eastern 36 parts, wall soil, granatum 26 parts, pennywort 27 parts, hawthorn 26 parts, 26 parts, Chinese honey locust, 24 parts, Chinese gall, the tuber of dwarf lilyturf 24 parts, the root of kudzu vine 24 parts, the root of Chinese wild ginger 21 parts, the root of large-flowered skullcap 23 parts, 22 parts, Poria cocos, dusty yeast 9 parts, lotus nut 14 parts, dried orange peel 12 parts, clam meal 5 parts, reed rhizome 21 parts, moutan bark 20 parts, lophatherum gracile 15 parts, Gorgon fruit 19 parts, 18 parts, balsam pear, the membrane of a chicken's gizzard 10 parts, the bark of ash 17 parts, Asiatic plantain 15 parts, rhizoma atractylodis 14 parts, radix pseudostellariae 15 parts, smartweed 16 parts, wartwort 15 parts, cordate houttuynia 16 parts, sealwort 13 parts, earthworm powder 20 parts, 14 parts, clam worm powder, winter honey 9 parts, 14 parts, fish oil.
The preparation method of the feed addictive of the control sweetfish bacterial enteritis disease of the present embodiment is with embodiment 1.
Experimental result
Contrast experiment 1
Test site is selected in fishing ground, Qingdao, in order to verify result for the treatment of of the present invention, is testing the sweetfish suffering from enteritis disease:
The process of control group is as follows: with the sick fish 15-20 minute of the nitrofurazone dipping of 2ppm, every day 2 times, uses continuously until the recovery from illness of sick fish.
The process of experiment A group is as follows: throw something and feed after being mixed with the weight ratio of 1:20 with commercially available basal feed by the feed addictive of the control sweetfish bacterial enteritis disease of the embodiment of the present invention 1, until the recovery from illness of sick fish.
The process of experiment B group is as follows: throw something and feed after being mixed with the weight ratio of 1:20 with commercially available basal feed by the feed addictive of the control sweetfish bacterial enteritis disease of the embodiment of the present invention 2, until the recovery from illness of sick fish.
The process of experiment C group is as follows: throw something and feed after being mixed with the weight ratio of 1:20 with commercially available basal feed by the feed addictive of the control sweetfish bacterial enteritis disease of the embodiment of the present invention 3, until the recovery from illness of sick fish.
The process of experiment D group is as follows: throw something and feed after being mixed with the weight ratio of 1:20 with commercially available basal feed by the feed addictive of the control sweetfish bacterial enteritis disease of the embodiment of the present invention 4, until the recovery from illness of sick fish.
The process of experiment E group is as follows: throw something and feed after being mixed with the weight ratio of 1:20 with commercially available basal feed by the feed addictive of the control sweetfish bacterial enteritis disease of the embodiment of the present invention 5, until the recovery from illness of sick fish.
The process of experiment F group is as follows: throw something and feed after being mixed with the weight ratio of 1:20 with commercially available basal feed by the feed addictive of the control sweetfish bacterial enteritis disease of the embodiment of the present invention 6, until the recovery from illness of sick fish.
Experimental result is in table 1:
The feed addictive result for the treatment of of table 1 control of the present invention sweetfish bacterial enteritis disease
Experimental result shows: the feed addictive of control sweetfish bacterial enteritis disease of the present invention, the nosogenesis according to enteritis disease, the suitable component of selection, and organically combined, there is significant cooperative synergism effect, play the function of each component to greatest extent.The component of the feed addictive of control sweetfish bacterial enteritis disease of the present invention is environmental protection raw material, nontoxic to environment, not containing chemicals or antibiotic, be conducive to the sustainable health development of aquaculture industry, the healthy of people can not be affected after edible sweetfish of having cured, and feed addictive of the present invention is good to the curative effect of sweetfish enteritis disease, cure rate is high, make the survival rate of ill sweetfish to more than 90%, after medication 1-3 days, sweetfish starts progressively to recover to ingest, and ingestion frequency improves.In addition, the present invention uses safer, little to sweetfish damage, and after using the feed addictive for the treatment of sweetfish enteritis disease of the present invention, sweetfish Health restoration is fast, medication 2-5 days, and sweetfish can recover normal activity, and idle fund is strong and vigorous, ingests completely normal.
Contrast experiment 2
Normally to raise group for blank group.Experimental group process is as follows:
Experiment a group: throw something and feed after the feed addictive of the control sweetfish enteritis disease of the embodiment of the present invention 1 is mixed with the weight ratio of 1:35 with commercially available basal feed, throw something and feed continuously 20 days.
Experiment b group: throw something and feed after the feed addictive of the control sweetfish enteritis disease of the embodiment of the present invention 2 is mixed with the weight ratio of 1:35 with commercially available basal feed, throw something and feed continuously 20 days.
Experiment c group: throw something and feed after the feed addictive of the control sweetfish enteritis disease of the embodiment of the present invention 3 is mixed with the weight ratio of 1:35 with commercially available basal feed, throw something and feed continuously 20 days.
Experiment d group: throw something and feed after the feed addictive of the control sweetfish enteritis disease of the embodiment of the present invention 4 is mixed with the weight ratio of 1:35 with commercially available basal feed, throw something and feed continuously 20 days.
Experiment e group: throw something and feed after the feed addictive of the control sweetfish enteritis disease of the embodiment of the present invention 5 is mixed with the weight ratio of 1:35 with commercially available basal feed, throw something and feed continuously 20 days.
Experiment f group: throw something and feed after the feed addictive of the control sweetfish enteritis disease of the embodiment of the present invention 6 is mixed with the weight ratio of 1:35 with commercially available basal feed, throw something and feed continuously 20 days.
When experiment starts, each processed group puts into healthy sweetfish 10, after raising 20 days, adds the sweetfish that 1 is suffered from enteritis disease in each processed group by above-mentioned process, observes and records infection state after one week.The results are shown in Table 2:
The effect of the feed addictive prevention enteritis disease of table 2 control of the present invention sweetfish bacterial enteritis disease
From table 2, the feed addictive of control sweetfish bacterial enteritis disease of the present invention effectively can reduce the incidence rate of sweetfish enteritis disease, can improve the immunity of sweetfish, has good prevention effect to enteritis disease.
Bacteriostatic test checks
With nitrofurazone as control group, the feed addictive of the control sweetfish bacterial enteritis disease of embodiment of the present invention 1-embodiment 6 is that experimental group carries out antibacterial inspection.
Culture medium: LB culture medium.
Bacterial classification is: Aeromonas punctata.
Control group antibacterial liquid is prepared: be dissolved in by nitrofurazone in aseptic deionized water, concentration is 2ppm.
Experimental group antibacterial liquid is prepared: be dissolved in respectively in aseptic deionized water by the feed addictive of the control sweetfish bacterial enteritis disease of embodiment of the present invention 1-embodiment 6, liquor strength being diluted to concentration is 2ppm.
Observe and record experimental result:
Represent with "-" without inhibition zone;
Antibacterial circle diameter < 10mm is slightly antibacterial, represents with "+";
Antibacterial circle diameter 10 ~ 14mm is that moderate is antibacterial, represents with " ++ ";
Antibacterial circle diameter > 14mm is highly antibacterial, represents with " +++ "; To the fungistatic effect of Aeromonas punctata in table 3:
The bactericidal and bacteriostatic effect of the feed addictive of table 3 control of the present invention sweetfish bacterial enteritis disease
From table 3, the pathogen Aeromonas punctata of feed addictive to enteritis disease of control sweetfish bacterial enteritis disease of the present invention has good bactericidal and bacteriostatic effect.
The above is only the preferred embodiment of the present invention, is noted that for those skilled in the art, and under the premise without departing from the principles of the invention, can also make some improvement, these improvement should be considered as protection scope of the present invention.
Claims (8)
1. prevent and treat the feed addictive of sweetfish bacterial enteritis disease for one kind, it is characterized in that: be made up of each component of following masses number: eastern wall soil 32-40 part, granatum 24-30 part, pennywort 24-30 part, hawthorn 23-30 part, Chinese honey locust 22-30 part, Chinese gall 21-28 part, the tuber of dwarf lilyturf 19-27 part, root of kudzu vine 19-27 part, root of Chinese wild ginger 18-25 part, root of large-flowered skullcap 18-26 part, Poria cocos 17-25 part, dusty yeast 6-14 part, lotus nut 8-17 part, dried orange peel 8-15 part, clam meal 4-5 part, reed rhizome 16-26 part, moutan bark 14-24 part, lophatherum gracile 12-20 part, Gorgon fruit 15-22 part, balsam pear 15-21 part, the membrane of a chicken's gizzard 6-15 part, bark of ash 14-21 part, Asiatic plantain 12-20 part, rhizoma atractylodis 11-18 part, radix pseudostellariae 12-18 part, smartweed 13-20 part, wartwort 12-19 part, cordate houttuynia 10-20 part, sealwort 9-18 part, earthworm powder 15-23 part, clam worm powder 10-20 part, winter honey 7-13 part, fish oil 8-16 part.
2. the feed addictive of control sweetfish bacterial enteritis disease according to claim 1, it is characterized in that: the feed addictive of described control sweetfish bacterial enteritis disease, be made up of each component of following masses number: eastern 35 parts, wall soil, granatum 28 parts, pennywort 28 parts, hawthorn 27 parts, 25 parts, Chinese honey locust, 25 parts, Chinese gall, the tuber of dwarf lilyturf 23 parts, the root of kudzu vine 22 parts, the root of Chinese wild ginger 22 parts, the root of large-flowered skullcap 21 parts, 20 parts, Poria cocos, dusty yeast 10 parts, lotus nut 12 parts, dried orange peel 13 parts, clam meal 4 parts, reed rhizome 20 parts, moutan bark 18 parts, lophatherum gracile 16 parts, Gorgon fruit 18 parts, 18 parts, balsam pear, the membrane of a chicken's gizzard 9 parts, the bark of ash 18 parts, Asiatic plantain 16 parts, rhizoma atractylodis 15 parts, radix pseudostellariae 15 parts, smartweed 17 parts, wartwort 16 parts, cordate houttuynia 15 parts, sealwort 14 parts, earthworm powder 18 parts, 15 parts, clam worm powder, winter honey 10 parts, 12 parts, fish oil.
3. the feed addictive of control sweetfish bacterial enteritis disease according to claim 1, it is characterized in that: the feed addictive of described control sweetfish bacterial enteritis disease, be made up of each component of following masses number: eastern 36 parts, wall soil, granatum 26 parts, pennywort 27 parts, hawthorn 26 parts, 26 parts, Chinese honey locust, 24 parts, Chinese gall, the tuber of dwarf lilyturf 24 parts, the root of kudzu vine 24 parts, the root of Chinese wild ginger 21 parts, the root of large-flowered skullcap 23 parts, 22 parts, Poria cocos, dusty yeast 9 parts, lotus nut 14 parts, dried orange peel 12 parts, clam meal 5 parts, reed rhizome 21 parts, moutan bark 20 parts, lophatherum gracile 15 parts, Gorgon fruit 19 parts, 18 parts, balsam pear, the membrane of a chicken's gizzard 10 parts, the bark of ash 17 parts, Asiatic plantain 15 parts, rhizoma atractylodis 14 parts, radix pseudostellariae 15 parts, smartweed 16 parts, wartwort 15 parts, cordate houttuynia 16 parts, sealwort 13 parts, earthworm powder 20 parts, 14 parts, clam worm powder, winter honey 9 parts, 14 parts, fish oil.
4. a preparation method for the feed addictive of the control sweetfish bacterial enteritis disease according to any one of claim 1-3, is characterized in that: comprise the following steps:
(1) take eastern wall soil and clam meal according to proportioning, eastern wall soil is mixed with clam meal, at 120 ~ 160 DEG C of frying 5-8 minute, puts into pulverizer after frying completes and pulverize and cross 100 mesh sieves, obtain comminuted powder;
(2) by mass concentration be 50% alcohol add in Chinese medicine extracting tank, alcohol consumption is 10 times of the comminuted powder weight of step (1) gained, open and stir, the comminuted powder of step (1) gained is added in Chinese medicine extracting tank, temperature of charge in Chinese medicine extracting tank is warming up to 77 DEG C of constant temperature and extracts 30min, 100 orders cross leaching filtrate, and filter residue returns in Chinese medicine extracting tank and repeats to extract once, 100 orders cross leaching filtrate, merge twice gained filtrate;
(3) granatum, pennywort, hawthorn, Chinese honey locust, Chinese gall, the tuber of dwarf lilyturf, the root of kudzu vine, the root of Chinese wild ginger, the root of large-flowered skullcap, Poria cocos, dried orange peel, reed rhizome, moutan bark and lophatherum gracile is taken according to proportioning, gross weight is designated as 1 part, add 6 parts of purified water, 70-80 DEG C is soaked 6-9h, obtains soak;
(4) boil 2h after the soak that step (3) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 4 parts in medicinal material, continue to boil 1h after being heated to boiling, merge twice fried liquid and be extract;
(5) take lotus nut, Gorgon fruit, balsam pear, the membrane of a chicken's gizzard, the bark of ash, Asiatic plantain, rhizoma atractylodis, radix pseudostellariae, smartweed, wartwort, cordate houttuynia and sealwort respectively according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(6) grounds travel that extract step (4) obtained and step (5) obtain is positioned in the extraction kettle in supercritical extract instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(7) take dusty yeast, earthworm powder, clam worm powder, winter honey and fish oil respectively according to proportioning, mix with the filtrate of step (2) gained, the extract of step (6) gained, obtain object.
5. the preparation method of the feed addictive of control sweetfish bacterial enteritis disease according to claim 4, it is characterized in that: the preparation method of described earthworm powder is: the earthworm freeze drying cleaned up is ground on ice to finish-drying, after 50 mesh sieves, obtain earthworm powder.
6. the preparation method of the feed addictive of control sweetfish bacterial enteritis disease according to claim 4, it is characterized in that: the preparation method of described clam worm powder is: the clam worm freeze drying cleaned up is ground on ice to finish-drying, after 50 mesh sieves, obtain clam worm powder.
7. the using method of the feed addictive of the control sweetfish bacterial enteritis disease according to any one of claim 1-3, is characterized in that: throw something and feed after being mixed with the weight ratio of 1:35-1:10 with commercially available basal feed by the feed addictive of control sweetfish bacterial enteritis disease of the present invention.
8. the using method of the feed addictive of control sweetfish bacterial enteritis disease according to claim 7, is characterized in that: the feed addictive of control sweetfish enteritis disease of the present invention and the weight ratio of commercially available basal feed are 1:20.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510971297.0A CN105558422A (en) | 2015-12-22 | 2015-12-22 | Feed additive with functions of prevention and treatment of bacillary enteritis of plecoglossus altivelis and preparation method of feed additive |
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| CN201510971297.0A CN105558422A (en) | 2015-12-22 | 2015-12-22 | Feed additive with functions of prevention and treatment of bacillary enteritis of plecoglossus altivelis and preparation method of feed additive |
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| CN106418001A (en) * | 2016-09-29 | 2017-02-22 | 青岛海之源智能技术有限公司 | Feed additive for treating bacterial enteritis disease of phractocephalus hemioliopterus, as well as preparation method thereof |
| CN106490402A (en) * | 2016-10-28 | 2017-03-15 | 青岛海之星生物科技有限公司 | A kind of feed addictive for treating colored perch red skin disease and preparation method thereof |
| CN106615644A (en) * | 2016-09-29 | 2017-05-10 | 青岛海之源智能技术有限公司 | Feed additive for preventing and treating sweetfish diseases due to Streptococcus and preparation method thereof |
| CN107019119A (en) * | 2017-03-25 | 2017-08-08 | 年付柱 | A kind of feed for preventing and treating catfish enteritis disease |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105029105A (en) * | 2015-08-28 | 2015-11-11 | 青岛海之源智能技术有限公司 | Low-cost and easy-to-eat mullet compound feed and manufacturing method thereof |
| CN105053624A (en) * | 2015-07-30 | 2015-11-18 | 青岛海之源智能技术有限公司 | Feed additive capable of promoting plecoglossus altivelis growth and preparation method thereof |
| CN105106725A (en) * | 2015-09-25 | 2015-12-02 | 青岛海之源智能技术有限公司 | Swordtail bacillary enteritis preventing feed additive and preparation method thereof |
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2015
- 2015-12-22 CN CN201510971297.0A patent/CN105558422A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053624A (en) * | 2015-07-30 | 2015-11-18 | 青岛海之源智能技术有限公司 | Feed additive capable of promoting plecoglossus altivelis growth and preparation method thereof |
| CN105029105A (en) * | 2015-08-28 | 2015-11-11 | 青岛海之源智能技术有限公司 | Low-cost and easy-to-eat mullet compound feed and manufacturing method thereof |
| CN105106725A (en) * | 2015-09-25 | 2015-12-02 | 青岛海之源智能技术有限公司 | Swordtail bacillary enteritis preventing feed additive and preparation method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106418001A (en) * | 2016-09-29 | 2017-02-22 | 青岛海之源智能技术有限公司 | Feed additive for treating bacterial enteritis disease of phractocephalus hemioliopterus, as well as preparation method thereof |
| CN106615644A (en) * | 2016-09-29 | 2017-05-10 | 青岛海之源智能技术有限公司 | Feed additive for preventing and treating sweetfish diseases due to Streptococcus and preparation method thereof |
| CN106490402A (en) * | 2016-10-28 | 2017-03-15 | 青岛海之星生物科技有限公司 | A kind of feed addictive for treating colored perch red skin disease and preparation method thereof |
| CN107019119A (en) * | 2017-03-25 | 2017-08-08 | 年付柱 | A kind of feed for preventing and treating catfish enteritis disease |
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Application publication date: 20160511 |