CN105410481A - Feed additive for preventing and treating turbot fin rot and preparation method thereof - Google Patents

Feed additive for preventing and treating turbot fin rot and preparation method thereof Download PDF

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Publication number
CN105410481A
CN105410481A CN201510973793.XA CN201510973793A CN105410481A CN 105410481 A CN105410481 A CN 105410481A CN 201510973793 A CN201510973793 A CN 201510973793A CN 105410481 A CN105410481 A CN 105410481A
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parts
turbot
treating
preventing
fin rot
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王芬
李洪
孙明
王亮
宋辉
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Qingdao Haizhiyuan Intelligent Technology Co Ltd
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Qingdao Haizhiyuan Intelligent Technology Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

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Abstract

The invention belongs to the technical field of aquaculture, relates to a feed additive for preventing and treating turbot fin rot and also relates to a preparation method of the feed additive. The feed additive is composed of the following components, by weight: 35-42 parts of duckweed, 26-33 parts of cortex pseudolaricis, 26-33 parts of houttuynia cordata, 26-32 parts of lycoris radiata, 26-32 parts of fructus cnidii, 24-30 parts of prunella vulgaris, 23-30 parts of astragalus sinicus, 8-16 parts of honeycomb, 22-29 parts of muskmelon pedicel, 19-27 parts of seeds of grapes, 17-25 parts of ephedra, 18-24 parts of asarum, 18-24 parts of roots of oriental stephania root, 16-23 parts of divaricate saposhnikovia, 15-22 parts of angelica decursiva, 14-22 parts of scutellaria baicalensis and 14-20 parts of abrus precatorius. The feed additive can effectively prevent and treat the turbot fin rot.

Description

Prevent and treat feed addictive of turbot fin rot and preparation method thereof
Technical field
The invention belongs to technical field of aquaculture, particularly relate to a kind of feed addictive preventing and treating turbot fin rot; The invention still further relates to a kind of above-mentioned preparation method preventing and treating the feed addictive of turbot fin rot.
Background technology
Turbot, also known as " turbot ", belong to Pleuronectiformes Bothidae, English name Turbot, is commonly called as brill, and health is flat approximate round, and eyes are positioned at left side, have a side to be blue or green brown, a small amount of prickle of tool; The sliding white of anophthalmia sidelight, dorsal fin and stern are without hardware and more long-living length is rapid, and meat flavour is delicious, has higher economic worth.The nineties in 20th century, turbot was introduced into China, had become one of Variety comprehensive of the coastal industrial aquaculture of northern China now.Turbot belongs to Northern Europe cold water fish, and comparatively strict to seawater index requests such as temperature, turbot maximum fatal temperature is 28 ~ 30 DEG C, minimum lethal temperature is 1 ~ 2 DEG C, optimum growth temperature is 14 ~ 17 DEG C, and it is up to 40 ‰ to the tolerance of temperature, and minimum is 12 ‰.
The cause of disease of turbot fin rot is Vibrio anguillarum, and the first feculence of the dorsal fin of sick fish, anal fin, pectoral fin and tail fin is white, congested rubescent until fester gradually after 1-2 days, time serious, fin tissue rots and defect, this disease is multiple is born in seedling stage and forms the initial stage, and propagation is fast, the death rate is high, belongs to acute death.Turbot fin rot is popular in summer more, there is no the medicine of special for treating turbot fin rot at present, the common method for the treatment of of treatment turbot fin rot is thrown something and fed for the dipping bath or add in feed of splashing with antibiotic, its shortcoming is that result for the treatment of is not good, toxicity is larger, toxic residual in turbot body, not only environment easily polluted, affect the sustainable health development of culture fishery, and the turbot after edible healing also can affect the healthy of people.Therefore, develop a kind of green non-poisonous, effectively the medicine of prevention and therapy turbot fin rot or feed addictive can have important economic implications and market prospects.
Summary of the invention
An object of the present invention is to provide a kind of feed addictive preventing and treating turbot fin rot, and its raw material components is green pure natural material, and easy to use, has good prevention and therapy effect to turbot fin rot.
In order to solve the problems of the technologies described above, the present invention is by the following technical solutions: a kind of feed addictive preventing and treating turbot fin rot, be made up of each component of following masses number: duckweed 35-42 part, Golden Larch Bark 26-33 part, cordate houttuynia 26-33 part, short-tube lycoris 26-32 part, frutus cnidii 26-32 part, selfheal 24-30 part, Chinese milk vetch 23-30 part, honeycomb 8-16 part, muskmelon pedicel 22-29 part, grape pip 19-27 part, Chinese ephedra 17-25 part, root of Chinese wild ginger 18-24 part, stephanotis 18-24 part, windproof 16-23 part, RADIX PEUCEDANI 15-22 part, root of large-flowered skullcap 14-22 part, jequirity 14-20 part, Chinese honey locust 13-18 part, dandelion 10-18 part, kalimeris 10-18 part, peppermint oil 8-16 part, light-coloured vinegar 7-16 part, surf clan shell powder 10-18 part, dusty yeast 3-6 part, euphausia superba powder 12-19 part, earthworm powder 11-18 part, fish oil 8-15 part.
Preferably, the described feed addictive preventing and treating turbot fin rot, be made up of each component of following masses number: 38 parts, duckweed, Golden Larch Bark 29 parts, cordate houttuynia 30 parts, short-tube lycoris 30 parts, frutus cnidii 28 parts, selfheal 27 parts, Chinese milk vetch 26 parts, 13 parts, honeycomb, muskmelon pedicel 25 parts, grape pip 24 parts, 22 parts, Chinese ephedra, the root of Chinese wild ginger 21 parts, stephanotis 22 parts, windproof 20 parts, RADIX PEUCEDANI 18 parts, the root of large-flowered skullcap 19 parts, jequirity 16 parts, 15 parts, Chinese honey locust, dandelion 15 parts, kalimeris 14 parts, peppermint oil 12 parts, light-coloured vinegar 10 parts, surf clan shell powder 15 parts, dusty yeast 4 parts, euphausia superba powder 15 parts, earthworm powder 14 parts, 10 parts, fish oil.
Preferably, the described feed addictive preventing and treating turbot fin rot, be made up of each component of following masses number: 39 parts, duckweed, Golden Larch Bark 30 parts, cordate houttuynia 28 parts, short-tube lycoris 29 parts, frutus cnidii 30 parts, selfheal 27 parts, Chinese milk vetch 27 parts, 12 parts, honeycomb, muskmelon pedicel 26 parts, grape pip 23 parts, 20 parts, Chinese ephedra, the root of Chinese wild ginger 22 parts, stephanotis 22 parts, windproof 19 parts, RADIX PEUCEDANI 19 parts, the root of large-flowered skullcap 18 parts, jequirity 17 parts, 15 parts, Chinese honey locust, dandelion 14 parts, kalimeris 14 parts, peppermint oil 13 parts, light-coloured vinegar 12 parts, surf clan shell powder 14 parts, dusty yeast 5 parts, euphausia superba powder 16 parts, earthworm powder 15 parts, 12 parts, fish oil.
Each component effect of the present invention is resolved as follows:
Duckweed is also known as blue or green duckweed, Tian Ping, duckweed grass, water duckweed, duckweed grass, and be Lemnaceae water surface floating plant, be used as medicine with whole herb with root, cold in nature, taste is pungent, function sweating promoting eruption, clearing away heat and promoting diuresis, cures mainly the diseases such as table evil heating, measles, oedema, can pest-resistant sterilization.Golden Larch Bark is dry root skin or the near root bark of pinaceae plant golden larch, can desinsection, antipruritic, antimycotic.Cordate houttuynia taste is pungent, cold nature; Return lung, bladder, large intestine channel, have clearing heat and detoxicating, reduce phlegm removing pus and relieving carbuncle, and the effect that inducing diuresis to reduce edema is treating stranguria, cures mainly dyspnea and cough due to lung-heat, lung carbuncle pyemesis, tonsillitis, hot dysentery, malaria, oedema, carbuncle sore tumefacting virus, and heat is drenched, eczema, the illnesss such as prolapse of the anus.The certain active anticancer of short-tube lycoris tool, and can anti-inflammatory, antipyretic, calm and emetic.Frutus cnidii is warm in nature, bitter, and energy heat-clearing and damp-drying drug, dispellings wind and arresting itching, be applicable to neurodermatitis, pruitus, chronic eczema, ringworm etc.The decoction of selfheal has obvious antiinflammatory action; The decoction of selfheal all has certain inhibitory action to shigella dysenteriae, typhoid bacillus, comma bacillus, Escherichia coli, proteus, staphylococcus and Bacillus tuberculosis in vitro.Chinese milk vetch has dispelling pathogenic wind for improving eyesight, strengthening the spleen and replenishing qi, the effect of removing toxic substances pain relieving.Honeycomb has detoxicating and destroying parasites, and effect of wind-expelling pain-stopping, is usually used in sore swollen toxin, acute mastitis, scrofula, skin stubborn dermatitis, the fungal infection of the hand, toothache, arthralgia due to wind-dampness.Muskmelon pedicel returns spleen; Stomach; Liver Channel, cures mainly apoplexy; Epilepsy; Larynx numbness; Accumulation and obstruction of sputum; It is unfavorable to breathe; Indigestion; Chest gastral cavity distending pain; Jaundice with damp-heat pathogen.Grape pip can stop the destruction of radical pair human body cell; protection Human autopsy tissues, prevents and treats more than the 100 kind of diseases caused by free radical such as heart disease, cancer, early ageing, diabetes, artery sclerosis, can also reduce blood fat; protection skin, energy sterilization, antiallergy.Chinese ephedra is warm in nature, pungent, the micro-hardship of taste, has Pulvis Diaphoreticus to tremble with fear, freeings lung and relieving asthma, effect of inducing diuresis for removing edema, can treat anemofrigid cold, the illnesss such as geomantic omen edema, bronchial astehma of breathing with cough uncomfortable in chest.The root of Chinese wild ginger can induce sweat loose cold, and wind-expelling pain-stopping is sensible, warming lung to reduce watery phlegm.Stephanotis is clearing heat and detoxicating, cooling blood and hemostasis, and eliminating stasis to subdue swelling can be used for oxyhepatitis, bacillary dysentery, acute appendicitis, stomachache, internal haemorrhage, traumatic injury, venomous snake bite; Mumps is controlled in external application, lymphnoditis, neurodermatitis.Anti-local flavor is pungent, sweet, and slightly warm in nature expels pathogenic wind from the body surface, removing dampness to relieve pain, only effect of convulsion.RADIX PEUCEDANI is herbaceos perennial, belongs to Umbelliferae, has spasmolysis of eliminating the phlegm, platelet aggregation-against and antiinflammatory action, and the metabolism and growth effect of inhibition cancer cell.Root of large-flowered skullcap bitter, cold in nature, there are heat-clearing and damp-drying drug, purging intense heat and detonicating, hemostasis, the effect such as antiabortive, cure mainly warm heat disease, the infection of the upper respiratory tract, cough with lung heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, hot eyes, fetal irritability, hypertension, carbuncle swell the diseases such as furuncle sore, the clinical antibiotic property of the root of large-flowered skullcap is better than the coptis, and does not develop immunity to drugs.Jequirity external application can control skin disease, and root, rattan are used as medicine, can be clearing heat and detoxicating, and diuresis, eliminates the phlegm, desinsection, cures mainly pain sore, mumps, mange, treating rheumatic ostealgia.Chinese honey locust is containing triterpenoid saponin (gledinin, gleditschiasaponin), tannin, ceryl alcohol, nonacosane, stigmasterol, sitosterol etc., the function of have eliminating the phlegm, having one's ideas straightened out, saponin(e element contained in Chinese honey locust is triterpene class and compound sugar, there are anti-inflammatory, antiulcer, disease-resistant change effect, also have anticancer and improve the effects such as AIDS immunity.Dandelion taste is sweet, and flat, nontoxic, clearing heat and detoxicating, diuresis dissipating bind, cures mainly acute mastitis, adenolymphitis, scrofula, malignant boil carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infections.Kalimeris taste is pungent, cold nature, cures mainly and returns liver, kidney, stomach, large intestine channel, and relieve internal heat anticancer, cool blood scattered silt, clearing heat and promoting diuresis, swelling and pain relieving, can be clearing heat and detoxicating, loose stasis of blood hemostasis, dampness removing, helps digestion, disappear long-pending, for cat fever, cough, acpuei pharyngitis, tonsillitis, mumps, catarrhal jaundice, taste-blindness rate, infantile malnutrition, enteritis, dysentery, spit blood, uterine bleeding, irregular menstruation; Sore furuncle pain is controlled in external application, mastitis, traumatism and bleeding; Dysentery or damp-heat diarrhea; Abscess of throat, carbuncle swells sore; Blood-head bleeding from five sense organs or subcutaneous tissue, to have blood in stool; Jaundice with damp-heat pathogen; Or oedema, difficult urination; Eating accumulation, abdominal fullness and distention.Peppermint oil has the merit declaring malicious promoting eruption, can be used for controlling wind-heat bundle table, measles without adequate eruption; Also can treat rubella itch, get the effect that its promoting eruption of dispeling the wind is antipruritic.Light-coloured vinegar can whet the appetite, and promotes the secretion of saliva and gastric juice, help digest absorption, make honey stomach, promoting digestion and removing indigestion, and light-coloured vinegar has well antibacterial and bactericidal action.Nutrition contained in surf clan shell powder is relatively more comprehensive, high protein, high-micro-element, high ferro, high calcium, few fat, delicious flavour, has nourshing Yin and drynsessmoistening prescription, inducing diuresis to reduce edema, softening and resolving hard mass effect.Dusty yeast is rich in various trace elements, is the natural flavouring that a kind of nutritive value is very high, and dusty yeast can also promote to digest and assimilate in addition.Containing abundant calcium in krill body, phosphorus and abundant krill astacin, its astacin is 40 times more than of common fish meal, and protein content is high in krill body, it is the natural feed of many fish and shellfish, the palatability of krill shows the raw glycogen amino acid that can stimulate certain seed amino acid of sense of smell and the sense of taste and appetite be had to facilitation, and in addition, the enzymatic contained in krill body enters the absorption and digestion of fish.Earthworm is nutritious, and has food calling effect to turbot.Fish oil can not only provide energy for turbot and have food calling effect.
Another object of the present invention is to provide a kind of above-mentioned preparation method preventing and treating the feed addictive of turbot fin rot, and this preparation method preventing and treating the feed addictive of turbot fin rot comprises the following steps:
(1) take duckweed and dandelion according to proportioning, add the purified water of 4 times that parts by weight are duckweed and dandelion parts by weight, 70-80 DEG C is soaked 4-6h, filters to obtain infusion;
(2) take Golden Larch Bark, cordate houttuynia, short-tube lycoris, frutus cnidii, selfheal and Chinese milk vetch according to proportioning, gross weight is designated as 1 part, adds 10 parts of purified water, and 20-30 DEG C is soaked 6-9h, obtain soak;
(3) boil 2h after the soak that step (2) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 6 parts in medicinal material, continue to boil 1h after being heated to boiling, merge twice fried liquid and be extract;
(4) take honeycomb, muskmelon pedicel, grape pip, Chinese ephedra, the root of Chinese wild ginger, stephanotis, windproof, RADIX PEUCEDANI, the root of large-flowered skullcap and jequirity respectively according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(5) grounds travel that extract step (3) obtained and step (4) obtain is positioned in the extraction kettle in supercritical extract instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(6) take Chinese honey locust and kalimeris respectively according to proportioning, smash to pieces respectively and get gained juice;
(7) peppermint oil, light-coloured vinegar, surf clan shell powder, dusty yeast, euphausia superba powder, earthworm powder and fish oil is taken respectively according to proportioning, mix with the juice of the infusion of step (1) gained, the extract of step (5) gained and step (6) gained, obtain object.
Preferably, described surf clan shell powder preparation method is: by clam meat autoclaving after 15-30 minute, 60 DEG C of oven dry in dryer, then pulverizes with pulverizer and cross 80 mesh sieves.Autoclaved object kills the pathogen may carried in clam meat.
Preferably, the preparation method of described earthworm powder is: the earthworm freeze drying cleaned up ground on ice to finish-drying, after 50 mesh sieves, obtain earthworm powder.Cryodesiccated advantage can not make the active material inactivation contained by earthworm in dry run, and the heat produced because constantly stirring when the advantage of grinding on ice is and reduces grinding, to keep temperature, protective tissue.
Usage and dosage: throw something and feed after the feed addictive preventing and treating turbot fin rot of the present invention is mixed with the weight ratio of 1:30-1:10 with commercially available basal feed, preferably, the weight ratio of the feed addictive and commercially available basal feed of preventing and treating turbot fin rot of the present invention is 1:20.
In preparation method, be that the active ingredient of duckweed and dandelion is fully separated out by duckweed and dandelion in the object that 70-80 DEG C is soaked 4-6h; By purified to Golden Larch Bark, cordate houttuynia, short-tube lycoris, frutus cnidii, selfheal and Chinese milk vetch water soaking a period of time, medicinal material deliquescing, cell expansion, active ingredient is easily fried, and medicinal material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medicinal material surface, protein coagulating, the capillary channel on blocking medicinal material surface, make the moisture when decocting enter not go, active ingredient is not easily fried, affect curative effect of medication, decoction can change the irritation effect of medicine, relatively mildly acts on turbot, promotes that turbot is to the absorption of medicine; Honeycomb, muskmelon pedicel, grape pip, Chinese ephedra, the root of Chinese wild ginger, stephanotis, windproof, RADIX PEUCEDANI, the root of large-flowered skullcap and jequirity grinding can be made the starch in medicinal material, protein separates out more fully at subsequent step; Supercritical extract step can retain the active ingredient of each component in the present invention to greatest extent; Chinese honey locust and kalimeris are smashed to pieces, the advantage of getting gained juice is that preparation method is simple, saves medicinal raw material, improves the utilization rate to Chinese honey locust and kalimeris, and the active ingredient of abundant Chinese honey locust and kalimeris, the better effects if of bactericidal.
Because the surf clan shell powder, euphausia superba powder and the earthworm powder that contain in feed addictive of the present invention are nutritious, delicious flavour, the overall mouthfeel of feed can be improved, fish oil for turbot makeup energy and can not only have food calling effect, dusty yeast is rich in various trace elements, be the natural flavouring that a kind of nutritive value is very high, dusty yeast can also promote to digest and assimilate in addition.Surf clan shell powder, dusty yeast, euphausia superba powder, earthworm powder and fish oil have collaborative food calling effect to turbot, therefore the feed addictive preventing and treating turbot fin rot of the present invention can improve the mouthfeel of basal feed, stimulate the taste bud of turbot, induction turbot is ingested and be with the addition of the basal feed of feed addictive of the present invention, and fully absorb the active ingredient that can strengthen turbot immunity and resistance in feed addictive, therefore turbot immunity and resistance can be strengthened in feed addictive of the present invention, the composition pathogen of fin rot to remarkable bactericidal and bacteriostatic effect can enter in turbot body, and play it and act on accordingly.
The feed addictive preventing and treating turbot fin rot of the present invention is the nosogenesis according to bacillary fin rot, the suitable component of selection, and is organically combined, and has significant cooperative synergism effect, plays the function of each component to greatest extent.Component of preventing and treating the feed addictive of turbot fin rot of the present invention is environmental protection raw material, nontoxic to environment, not containing chemicals or antibiotic, is conducive to the sustainable health development of culture fishery; The healthy of people can not be affected after edible turbot of having cured, and feed addictive of the present invention is good to the curative effect of turbot fin rot, cure rate is high, makes the survival rate of ill turbot to more than 95%, after medication 1-2 days, rotten fin phenomenon suppresses to some extent.The present invention uses safer; little to turbot damage; after using the feed addictive for the treatment of turbot fin rot of the present invention; turbot Health restoration is fast, and use after 2-5 days, turbot can recover normal activity; idle fund is strong and vigorous; wound progressively heals, and fin ray progressively recovers normal, ingests completely normal.In addition, the feed addictive preventing and treating turbot fin rot of the present invention effectively can also prevent the generation of turbot fin rot.
Compared with prior art, the invention has the beneficial effects as follows:
(1), in each component of the present invention, duckweed, also known as blue or green duckweed, Tian Ping, duckweed grass, water duckweed, duckweed grass, is Lemnaceae water surface floating plant, be used as medicine with whole herb with root, cold in nature, taste is pungent, function sweating promoting eruption, clearing away heat and promoting diuresis, cure mainly the diseases such as table evil heating, measles, oedema, can pest-resistant sterilization.Golden Larch Bark is dry root skin or the near root bark of pinaceae plant golden larch, can desinsection, antipruritic, antimycotic.Cordate houttuynia taste is pungent, cold nature; Return lung, bladder, large intestine channel, have clearing heat and detoxicating, reduce phlegm removing pus and relieving carbuncle, and the effect that inducing diuresis to reduce edema is treating stranguria, cures mainly dyspnea and cough due to lung-heat, lung carbuncle pyemesis, tonsillitis, hot dysentery, malaria, oedema, carbuncle sore tumefacting virus, and heat is drenched, eczema, the illnesss such as prolapse of the anus.The certain active anticancer of short-tube lycoris tool, and can anti-inflammatory, antipyretic, calm and emetic.Frutus cnidii is warm in nature, bitter, and energy heat-clearing and damp-drying drug, dispellings wind and arresting itching, be applicable to neurodermatitis, pruitus, chronic eczema, ringworm etc.The decoction of selfheal has obvious antiinflammatory action; The decoction of selfheal all has certain inhibitory action to shigella dysenteriae, typhoid bacillus, comma bacillus, Escherichia coli, proteus, staphylococcus and Bacillus tuberculosis in vitro.Chinese milk vetch has dispelling pathogenic wind for improving eyesight, strengthening the spleen and replenishing qi, the effect of removing toxic substances pain relieving.Honeycomb has detoxicating and destroying parasites, and effect of wind-expelling pain-stopping, is usually used in sore swollen toxin, acute mastitis, scrofula, skin stubborn dermatitis, the fungal infection of the hand, toothache, arthralgia due to wind-dampness.Muskmelon pedicel returns spleen; Stomach; Liver Channel, cures mainly apoplexy; Epilepsy; Larynx numbness; Accumulation and obstruction of sputum; It is unfavorable to breathe; Indigestion; Chest gastral cavity distending pain; Jaundice with damp-heat pathogen.Grape pip can stop the destruction of radical pair human body cell; protection Human autopsy tissues, prevents and treats more than the 100 kind of diseases caused by free radical such as heart disease, cancer, early ageing, diabetes, artery sclerosis, can also reduce blood fat; protection skin, energy sterilization, antiallergy.Chinese ephedra is warm in nature, pungent, the micro-hardship of taste, has Pulvis Diaphoreticus to tremble with fear, freeings lung and relieving asthma, effect of inducing diuresis for removing edema, can treat anemofrigid cold, the illnesss such as geomantic omen edema, bronchial astehma of breathing with cough uncomfortable in chest.The root of Chinese wild ginger can induce sweat loose cold, and wind-expelling pain-stopping is sensible, warming lung to reduce watery phlegm.Stephanotis is clearing heat and detoxicating, cooling blood and hemostasis, and eliminating stasis to subdue swelling can be used for oxyhepatitis, bacillary dysentery, acute appendicitis, stomachache, internal haemorrhage, traumatic injury, venomous snake bite; Mumps is controlled in external application, lymphnoditis, neurodermatitis.Anti-local flavor is pungent, sweet, and slightly warm in nature expels pathogenic wind from the body surface, removing dampness to relieve pain, only effect of convulsion.RADIX PEUCEDANI is herbaceos perennial, belongs to Umbelliferae, has spasmolysis of eliminating the phlegm, platelet aggregation-against and antiinflammatory action, and the metabolism and growth effect of inhibition cancer cell.Root of large-flowered skullcap bitter, cold in nature, there are heat-clearing and damp-drying drug, purging intense heat and detonicating, hemostasis, the effect such as antiabortive, cure mainly warm heat disease, the infection of the upper respiratory tract, cough with lung heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, hot eyes, fetal irritability, hypertension, carbuncle swell the diseases such as furuncle sore, the clinical antibiotic property of the root of large-flowered skullcap is better than the coptis, and does not develop immunity to drugs.Jequirity external application can control skin disease, and root, rattan are used as medicine, can be clearing heat and detoxicating, and diuresis, eliminates the phlegm, desinsection, cures mainly pain sore, mumps, mange, treating rheumatic ostealgia.Chinese honey locust is containing triterpenoid saponin (gledinin, gleditschiasaponin), tannin, ceryl alcohol, nonacosane, stigmasterol, sitosterol etc., the function of have eliminating the phlegm, having one's ideas straightened out, saponin(e element contained in Chinese honey locust is triterpene class and compound sugar, there are anti-inflammatory, antiulcer, disease-resistant change effect, also have anticancer and improve the effects such as AIDS immunity.Dandelion taste is sweet, and flat, nontoxic, clearing heat and detoxicating, diuresis dissipating bind, cures mainly acute mastitis, adenolymphitis, scrofula, malignant boil carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infections.Kalimeris taste is pungent, cold nature, cures mainly and returns liver, kidney, stomach, large intestine channel, and relieve internal heat anticancer, cool blood scattered silt, clearing heat and promoting diuresis, swelling and pain relieving, can be clearing heat and detoxicating, loose stasis of blood hemostasis, dampness removing, helps digestion, disappear long-pending, for cat fever, cough, acpuei pharyngitis, tonsillitis, mumps, catarrhal jaundice, taste-blindness rate, infantile malnutrition, enteritis, dysentery, spit blood, uterine bleeding, irregular menstruation; Sore furuncle pain is controlled in external application, mastitis, traumatism and bleeding; Dysentery or damp-heat diarrhea; Abscess of throat, carbuncle swells sore; Blood-head bleeding from five sense organs or subcutaneous tissue, to have blood in stool; Jaundice with damp-heat pathogen; Or oedema, difficult urination; Eating accumulation, abdominal fullness and distention.Peppermint oil has the merit declaring malicious promoting eruption, can be used for controlling wind-heat bundle table, measles without adequate eruption; Also can treat rubella itch, get the effect that its promoting eruption of dispeling the wind is antipruritic.Light-coloured vinegar can whet the appetite, and promotes the secretion of saliva and gastric juice, help digest absorption, make honey stomach, promoting digestion and removing indigestion, and light-coloured vinegar has well antibacterial and bactericidal action.Nutrition contained in surf clan shell powder is relatively more comprehensive, high protein, high-micro-element, high ferro, high calcium, few fat, delicious flavour, has nourshing Yin and drynsessmoistening prescription, inducing diuresis to reduce edema, softening and resolving hard mass effect.Dusty yeast is rich in various trace elements, is the natural flavouring that a kind of nutritive value is very high, and dusty yeast can also promote to digest and assimilate in addition.Containing abundant calcium in krill body, phosphorus and abundant krill astacin, its astacin is 40 times more than of common fish meal, and protein content is high in krill body, it is the natural feed of many fish and shellfish, the palatability of krill shows the raw glycogen amino acid that can stimulate certain seed amino acid of sense of smell and the sense of taste and appetite be had to facilitation, and in addition, the enzymatic contained in krill body enters the absorption and digestion of fish.Earthworm is nutritious, and has food calling effect to turbot.Fish oil can not only provide energy for turbot and have food calling effect.
(2) feed addictive preventing and treating turbot fin rot of the present invention, is the Chinese herbal medicine selected according to the nosogenesis of fin rot, and is organically combined, have significant cooperative synergism effect, has played the function of each component to greatest extent.
(3) pathogen of feed addictive to turbot fin rot preventing and treating turbot fin rot of the present invention has good inhibition.
(4) component of preventing and treating the feed addictive of turbot fin rot of the present invention is environmental protection raw material, nontoxic to environment, without chemicals or antibiotic, is conducive to the sustainable health development of culture fishery; The healthy of people can not be affected after edible turbot of having cured, and the feed addictive preventing and treating turbot fin rot of the present invention damages very little to turbot, use of the present invention prevent and treat the feed addictive of turbot fin rot after, turbot Health restoration is fast.In addition, the feed addictive preventing and treating turbot fin rot of the present invention is easy to use, will not pull rear medicine-feeding out by sick fish, decrease the injury to sick fish in operating process.
(5) surf clan shell powder owing to containing in feed addictive of the present invention, euphausia superba powder and earthworm powder nutritious, delicious flavour, and the overall mouthfeel of feed can be improved, fish oil for turbot makeup energy and can not only have food calling effect, dusty yeast is rich in various trace elements, it is the natural flavouring that a kind of nutritive value is very high, in addition dusty yeast can also promote to digest and assimilate, surf clan shell powder, dusty yeast, euphausia superba powder, earthworm powder and fish oil have collaborative food calling effect to turbot, therefore the feed addictive preventing and treating turbot fin rot of the present invention can improve the mouthfeel of basal feed, stimulate the taste bud of turbot, induction turbot is ingested and be with the addition of the basal feed of feed addictive of the present invention, and fully absorb the active ingredient that can strengthen turbot immunity and resistance in feed addictive, therefore turbot immunity and resistance can be strengthened in feed addictive of the present invention, the composition pathogen of fin rot to remarkable bactericidal and bacteriostatic effect can enter in turbot body, and play it and act on accordingly.
(6), in preparation method, be that the active ingredient of duckweed and dandelion is fully separated out by duckweed and dandelion in the object that 70-80 DEG C is soaked 4-6h; By purified to Golden Larch Bark, cordate houttuynia, short-tube lycoris, frutus cnidii, selfheal and Chinese milk vetch water soaking a period of time, medicinal material deliquescing, cell expansion, active ingredient is easily fried, and medicinal material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medicinal material surface, protein coagulating, the capillary channel on blocking medicinal material surface, make the moisture when decocting enter not go, active ingredient is not easily fried, affect curative effect of medication, decoction can change the irritation effect of medicine, relatively mildly acts on turbot, promotes that turbot is to the absorption of medicine; Honeycomb, muskmelon pedicel, grape pip, Chinese ephedra, the root of Chinese wild ginger, stephanotis, windproof, RADIX PEUCEDANI, the root of large-flowered skullcap and jequirity grinding can be made the starch in medicinal material, protein separates out more fully at subsequent step; Supercritical extract step can retain the active ingredient of each component in the present invention to greatest extent; Chinese honey locust and kalimeris are smashed to pieces, the advantage of getting gained juice is that preparation method is simple, saves medicinal raw material, improves the utilization rate to Chinese honey locust and kalimeris, and the active ingredient of abundant Chinese honey locust and kalimeris, the better effects if of bactericidal.
Detailed description of the invention
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.
Embodiment 1
A kind of feed addictive preventing and treating turbot fin rot, be made up of each component of following masses number: 40 parts, duckweed, Golden Larch Bark 33 parts, cordate houttuynia 33 parts, short-tube lycoris 32 parts, frutus cnidii 32 parts, selfheal 28 parts, Chinese milk vetch 30 parts, 16 parts, honeycomb, muskmelon pedicel 29 parts, grape pip 27 parts, 25 parts, Chinese ephedra, the root of Chinese wild ginger 24 parts, stephanotis 23 parts, windproof 23 parts, RADIX PEUCEDANI 22 parts, the root of large-flowered skullcap 20 parts, jequirity 20 parts, 18 parts, Chinese honey locust, dandelion 18 parts, kalimeris 16 parts, peppermint oil 16 parts, light-coloured vinegar 14 parts, surf clan shell powder 18 parts, dusty yeast 5 parts, euphausia superba powder 18 parts, earthworm powder 18 parts, 15 parts, fish oil.
The above-mentioned preparation method preventing and treating the feed addictive of turbot fin rot comprises the following steps:
(1) take duckweed and dandelion according to proportioning, add the purified water of 4 times that parts by weight are duckweed and dandelion parts by weight, 70-80 DEG C is soaked 4-6h, filters to obtain infusion;
(2) take Golden Larch Bark, cordate houttuynia, short-tube lycoris, frutus cnidii, selfheal and Chinese milk vetch according to proportioning, gross weight is designated as 1 part, adds 10 parts of purified water, and 20-30 DEG C is soaked 6-9h, obtain soak;
(3) boil 2h after the soak that step (2) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 6 parts in medicinal material, continue to boil 1h after being heated to boiling, merge twice fried liquid and be extract;
(4) take honeycomb, muskmelon pedicel, grape pip, Chinese ephedra, the root of Chinese wild ginger, stephanotis, windproof, RADIX PEUCEDANI, the root of large-flowered skullcap and jequirity respectively according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(5) grounds travel that extract step (3) obtained and step (4) obtain is positioned in the extraction kettle in supercritical extract instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(6) take Chinese honey locust and kalimeris respectively according to proportioning, smash to pieces respectively and get gained juice;
(7) peppermint oil, light-coloured vinegar, surf clan shell powder, dusty yeast, euphausia superba powder, earthworm powder and fish oil is taken respectively according to proportioning, mix with the juice of the infusion of step (1) gained, the extract of step (5) gained and step (6) gained, obtain object.
Described surf clan shell powder preparation method is: by clam meat autoclaving after 15-30 minute, 60 DEG C of oven dry in dryer, then pulverizes with pulverizer and cross 80 mesh sieves.Autoclaved object kills the pathogen may carried in clam meat.
The preparation method of described earthworm powder is: the earthworm freeze drying cleaned up ground on ice to finish-drying, after 50 mesh sieves, obtain earthworm powder.Cryodesiccated advantage can not make the active material inactivation contained by earthworm in dry run, and the heat produced because constantly stirring when the advantage of grinding on ice is and reduces grinding, to keep temperature, protective tissue
Embodiment 2
A kind of feed addictive preventing and treating turbot fin rot, be made up of each component of following masses number: 42 parts, duckweed, Golden Larch Bark 32 parts, cordate houttuynia 32 parts, short-tube lycoris 32 parts, frutus cnidii 32 parts, selfheal 30 parts, Chinese milk vetch 28 parts, 14 parts, honeycomb, muskmelon pedicel 28 parts, grape pip 26 parts, 25 parts, Chinese ephedra, the root of Chinese wild ginger 24 parts, stephanotis 24 parts, windproof 22 parts, RADIX PEUCEDANI 21 parts, the root of large-flowered skullcap 22 parts, jequirity 18 parts, 17 parts, Chinese honey locust, dandelion 17 parts, kalimeris 18 parts, peppermint oil 15 parts, light-coloured vinegar 16 parts, surf clan shell powder 17 parts, dusty yeast 6 parts, euphausia superba powder 19 parts, earthworm powder 18 parts, 14 parts, fish oil.
The preparation method preventing and treating the feed addictive of turbot fin rot of the present embodiment is with embodiment 1.
Embodiment 3
A kind of feed addictive preventing and treating turbot fin rot, be made up of each component of following masses number: 35 parts, duckweed, Golden Larch Bark 28 parts, cordate houttuynia 27 parts, short-tube lycoris 26 parts, frutus cnidii 26 parts, selfheal 24 parts, Chinese milk vetch 23 parts, 10 parts, honeycomb, muskmelon pedicel 24 parts, grape pip 19 parts, 18 parts, Chinese ephedra, the root of Chinese wild ginger 18 parts, stephanotis 18 parts, windproof 16 parts, RADIX PEUCEDANI 15 parts, the root of large-flowered skullcap 15 parts, jequirity 14 parts, 14 parts, Chinese honey locust, dandelion 12 parts, kalimeris 10 parts, peppermint oil 9 parts, light-coloured vinegar 7 parts, surf clan shell powder 12 parts, dusty yeast 3 parts, euphausia superba powder 14 parts, earthworm powder 11 parts, 9 parts, fish oil.
The preparation method preventing and treating the feed addictive of turbot fin rot of the present embodiment is with embodiment 1.
Embodiment 4
A kind of feed addictive preventing and treating turbot fin rot, be made up of each component of following masses number: 36 parts, duckweed, Golden Larch Bark 26 parts, cordate houttuynia 26 parts, short-tube lycoris 27 parts, frutus cnidii 27 parts, selfheal 24 parts, Chinese milk vetch 24 parts, 8 parts, honeycomb, muskmelon pedicel 22 parts, grape pip 20 parts, 17 parts, Chinese ephedra, the root of Chinese wild ginger 18 parts, stephanotis 20 parts, windproof 18 parts, RADIX PEUCEDANI 15 parts, the root of large-flowered skullcap 14 parts, jequirity 15 parts, 13 parts, Chinese honey locust, dandelion 10 parts, kalimeris 12 parts, peppermint oil 8 parts, light-coloured vinegar 8 parts, surf clan shell powder 10 parts, dusty yeast 3 parts, euphausia superba powder 12 parts, earthworm powder 11 parts, 8 parts, fish oil.
The preparation method preventing and treating the feed addictive of turbot fin rot of the present embodiment is with embodiment 1.
Embodiment 5
A kind of feed addictive preventing and treating turbot fin rot, be made up of each component of following masses number: 38 parts, duckweed, Golden Larch Bark 29 parts, cordate houttuynia 30 parts, short-tube lycoris 30 parts, frutus cnidii 28 parts, selfheal 27 parts, Chinese milk vetch 26 parts, 13 parts, honeycomb, muskmelon pedicel 25 parts, grape pip 24 parts, 22 parts, Chinese ephedra, the root of Chinese wild ginger 21 parts, stephanotis 22 parts, windproof 20 parts, RADIX PEUCEDANI 18 parts, the root of large-flowered skullcap 19 parts, jequirity 16 parts, 15 parts, Chinese honey locust, dandelion 15 parts, kalimeris 14 parts, peppermint oil 12 parts, light-coloured vinegar 10 parts, surf clan shell powder 15 parts, dusty yeast 4 parts, euphausia superba powder 15 parts, earthworm powder 14 parts, 10 parts, fish oil.
The preparation method preventing and treating the feed addictive of turbot fin rot of the present embodiment is with embodiment 1.
Embodiment 6
A kind of feed addictive preventing and treating turbot fin rot, be made up of each component of following masses number: 39 parts, duckweed, Golden Larch Bark 30 parts, cordate houttuynia 28 parts, short-tube lycoris 29 parts, frutus cnidii 30 parts, selfheal 27 parts, Chinese milk vetch 27 parts, 12 parts, honeycomb, muskmelon pedicel 26 parts, grape pip 23 parts, 20 parts, Chinese ephedra, the root of Chinese wild ginger 22 parts, stephanotis 22 parts, windproof 19 parts, RADIX PEUCEDANI 19 parts, the root of large-flowered skullcap 18 parts, jequirity 17 parts, 15 parts, Chinese honey locust, dandelion 14 parts, kalimeris 14 parts, peppermint oil 13 parts, light-coloured vinegar 12 parts, surf clan shell powder 14 parts, dusty yeast 5 parts, euphausia superba powder 16 parts, earthworm powder 15 parts, 12 parts, fish oil.
The preparation method preventing and treating the feed addictive of turbot fin rot of the present embodiment is with embodiment 1.
Experimental result
Contrast experiment 1
Test site is selected in fishing ground, Qingdao, in order to verify result for the treatment of of the present invention, is testing the turbot suffering from fin rot:
The process of control group is as follows: with the sick fish 15-20 minute of the nitrofurazone dipping of 2ppm, every day 2 times, uses continuously until the recovery from illness of sick fish.
The process of experiment A group is as follows: throw something and feed after being mixed with the weight ratio of 1:20 with commercially available basal feed by the feed addictive preventing and treating turbot fin rot of embodiment 1, until the recovery from illness of sick fish.
The process of experiment B group is as follows: throw something and feed after being mixed with the weight ratio of 1:20 with commercially available basal feed by the feed addictive preventing and treating turbot fin rot of embodiment 2, until the recovery from illness of sick fish.
The process of experiment C group is as follows: throw something and feed after being mixed with the weight ratio of 1:20 with commercially available basal feed by the feed addictive preventing and treating turbot fin rot of embodiment 3, until the recovery from illness of sick fish.
The process of experiment D group is as follows: throw something and feed after being mixed with the weight ratio of 1:20 with commercially available basal feed by the feed addictive preventing and treating turbot fin rot of embodiment 4, until the recovery from illness of sick fish.
The process of experiment E group is as follows: throw something and feed after being mixed with the weight ratio of 1:20 with commercially available basal feed by the feed addictive preventing and treating turbot fin rot of embodiment 5, until the recovery from illness of sick fish.
The process of experiment F group is as follows: throw something and feed after being mixed with the weight ratio of 1:20 with commercially available basal feed by the feed addictive preventing and treating turbot fin rot of embodiment 6, until the recovery from illness of sick fish.
Experimental result is in table 1:
Table 1 feed addictive result for the treatment of of preventing and treating turbot fin rot of the present invention
Experimental result shows: the feed addictive preventing and treating turbot fin rot of the present invention, is the nosogenesis according to fin rot, the suitable component of selection, and is organically combined, and has significant cooperative synergism effect, plays the function of each component to greatest extent.Component of preventing and treating the feed addictive of turbot fin rot of the present invention is environmental protection raw material, nontoxic to environment, not containing chemicals or antibiotic, is conducive to the sustainable health development of culture fishery; The healthy of people can not be affected after edible turbot of having cured, and feed addictive of the present invention is good to the curative effect of turbot fin rot, cure rate is high, makes the survival rate of ill turbot to more than 95%, use after 1-2 days, rotten fin phenomenon suppresses to some extent.The present invention uses safer; little to turbot damage; after using the feed addictive for the treatment of turbot fin rot of the present invention; turbot Health restoration is fast, medication 2-5 days, and turbot can recover normal activity; idle fund is strong and vigorous; wound progressively heals, and fin ray progressively recovers normal, ingests completely normal.
Contrast experiment 2
Normally to raise group for blank group.Experimental group process is as follows:
Experiment a group: throw something and feed after being mixed with the weight ratio of 1:30 with commercially available basal feed by the feed addictive preventing and treating turbot fin rot of embodiment 1, until the recovery from illness of sick fish.
Experiment b group: throw something and feed after being mixed with the weight ratio of 1:30 with commercially available basal feed by the feed addictive preventing and treating turbot fin rot of embodiment 2, until the recovery from illness of sick fish.
Experiment c group: throw something and feed after being mixed with the weight ratio of 1:30 with commercially available basal feed by the feed addictive preventing and treating turbot fin rot of embodiment 3, until the recovery from illness of sick fish.
Experiment d group: throw something and feed after being mixed with the weight ratio of 1:30 with commercially available basal feed by the feed addictive preventing and treating turbot fin rot of embodiment 4, until the recovery from illness of sick fish.
Experiment e group: throw something and feed after being mixed with the weight ratio of 1:30 with commercially available basal feed by the feed addictive preventing and treating turbot fin rot of embodiment 5, until the recovery from illness of sick fish.
Experiment f group: throw something and feed after being mixed with the weight ratio of 1:30 with commercially available basal feed by the feed addictive preventing and treating turbot fin rot of embodiment 6, until the recovery from illness of sick fish.
When experiment starts, each processed group puts into healthy turbot 20, after raising 20 days, adds the turbot that 1 is suffered from fin rot in each processed group by above-mentioned process, observes and records infection state after one week.The results are shown in Table 2:
Table 2 feed addictive preventing and treating turbot fin rot of the present invention prevents the effect of bacillary fin rot
From table 2, the feed addictive preventing and treating turbot fin rot of the present invention effectively can reduce the incidence rate of turbot fin rot, can improve the immunity of turbot, has good prevention effect to bacillary fin rot.
Bacteriostatic test checks
With nitrofurazone as control group, the feed addictive preventing and treating turbot fin rot of embodiment of the present invention 1-embodiment 6 is that experimental group carries out antibacterial inspection contrast.
Culture medium: LB culture medium.
Bacterial classification is: Vibrio anguillarum.
Control group antibacterial liquid is prepared: being dissolved in by nitrofurazone in aseptic deionized water and being diluted to concentration is 2ppm.
Experimental group antibacterial liquid is prepared: be dissolved in respectively in aseptic deionized water by the feed addictive preventing and treating turbot fin rot of embodiment of the present invention 1-embodiment 6, liquor strength being diluted to concentration is 2ppm.
Observe and record experimental result:
Represent with "-" without inhibition zone;
Antibacterial circle diameter < 10mm is slightly antibacterial, represents with "+";
Antibacterial circle diameter 10 ~ 14mm is that moderate is antibacterial, represents with " ++ ";
Antibacterial circle diameter > 14mm is highly antibacterial, represents with " +++ "; To the fungistatic effect of Vibrio anguillarum in table 3:
Table 3 bactericidal and bacteriostatic effect preventing and treating the feed addictive of turbot fin rot of the present invention
From table 3, the pathogen Vibrio anguillarum of feed addictive to bacillary fin rot preventing and treating turbot fin rot of the present invention has good bactericidal and bacteriostatic effect.
The above is only the preferred embodiment of the present invention, is noted that for those skilled in the art, and under the premise without departing from the principles of the invention, can also make some improvement, these improvement should be considered as protection scope of the present invention.

Claims (8)

1. prevent and treat the feed addictive of turbot fin rot for one kind, it is characterized in that: be made up of each component of following masses number: duckweed 35-42 part, Golden Larch Bark 26-33 part, cordate houttuynia 26-33 part, short-tube lycoris 26-32 part, frutus cnidii 26-32 part, selfheal 24-30 part, Chinese milk vetch 23-30 part, honeycomb 8-16 part, muskmelon pedicel 22-29 part, grape pip 19-27 part, Chinese ephedra 17-25 part, root of Chinese wild ginger 18-24 part, stephanotis 18-24 part, windproof 16-23 part, RADIX PEUCEDANI 15-22 part, root of large-flowered skullcap 14-22 part, jequirity 14-20 part, Chinese honey locust 13-18 part, dandelion 10-18 part, kalimeris 10-18 part, peppermint oil 8-16 part, light-coloured vinegar 7-16 part, surf clan shell powder 10-18 part, dusty yeast 3-6 part, euphausia superba powder 12-19 part, earthworm powder 11-18 part, fish oil 8-15 part.
2. the feed addictive preventing and treating turbot fin rot according to claim 1, it is characterized in that: the described feed addictive preventing and treating turbot fin rot, be made up of each component of following masses number: 38 parts, duckweed, Golden Larch Bark 29 parts, cordate houttuynia 30 parts, short-tube lycoris 30 parts, frutus cnidii 28 parts, selfheal 27 parts, Chinese milk vetch 26 parts, 13 parts, honeycomb, muskmelon pedicel 25 parts, grape pip 24 parts, 22 parts, Chinese ephedra, the root of Chinese wild ginger 21 parts, stephanotis 22 parts, windproof 20 parts, RADIX PEUCEDANI 18 parts, the root of large-flowered skullcap 19 parts, jequirity 16 parts, 15 parts, Chinese honey locust, dandelion 15 parts, kalimeris 14 parts, peppermint oil 12 parts, light-coloured vinegar 10 parts, surf clan shell powder 15 parts, dusty yeast 4 parts, euphausia superba powder 15 parts, earthworm powder 14 parts, 10 parts, fish oil.
3. the feed addictive preventing and treating turbot fin rot according to claim 1, it is characterized in that: the described feed addictive preventing and treating turbot fin rot, be made up of each component of following masses number: 39 parts, duckweed, Golden Larch Bark 30 parts, cordate houttuynia 28 parts, short-tube lycoris 29 parts, frutus cnidii 30 parts, selfheal 27 parts, Chinese milk vetch 27 parts, 12 parts, honeycomb, muskmelon pedicel 26 parts, grape pip 23 parts, 20 parts, Chinese ephedra, the root of Chinese wild ginger 22 parts, stephanotis 22 parts, windproof 19 parts, RADIX PEUCEDANI 19 parts, the root of large-flowered skullcap 18 parts, jequirity 17 parts, 15 parts, Chinese honey locust, dandelion 14 parts, kalimeris 14 parts, peppermint oil 13 parts, light-coloured vinegar 12 parts, surf clan shell powder 14 parts, dusty yeast 5 parts, euphausia superba powder 16 parts, earthworm powder 15 parts, 12 parts, fish oil.
4. the preparation method preventing and treating the feed addictive of turbot fin rot according to any one of claim 1-3, is characterized in that: comprise the following steps:
(1) take duckweed and dandelion according to proportioning, add the purified water of 4 times that parts by weight are duckweed and dandelion parts by weight, 70-80 DEG C is soaked 4-6h, filters to obtain infusion;
(2) take Golden Larch Bark, cordate houttuynia, short-tube lycoris, frutus cnidii, selfheal and Chinese milk vetch according to proportioning, gross weight is designated as 1 part, adds 10 parts of purified water, and 20-30 DEG C is soaked 6-9h, obtain soak;
(3) boil 2h after the soak that step (2) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 6 parts in medicinal material, continue to boil 1h after being heated to boiling, merge twice fried liquid and be extract;
(4) take honeycomb, muskmelon pedicel, grape pip, Chinese ephedra, the root of Chinese wild ginger, stephanotis, windproof, RADIX PEUCEDANI, the root of large-flowered skullcap and jequirity respectively according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(5) grounds travel that extract step (3) obtained and step (4) obtain is positioned in the extraction kettle in supercritical extract instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(6) take Chinese honey locust and kalimeris respectively according to proportioning, smash to pieces respectively and get gained juice;
(7) peppermint oil, light-coloured vinegar, surf clan shell powder, dusty yeast, euphausia superba powder, earthworm powder and fish oil is taken respectively according to proportioning, mix with the juice of the infusion of step (1) gained, the extract of step (5) gained and step (6) gained, obtain object.
5. the preparation method preventing and treating the feed addictive of turbot fin rot according to claim 4, it is characterized in that: described surf clan shell powder preparation method is: by clam meat autoclaving after 15-30 minute, 60 DEG C of oven dry in dryer, then pulverize with pulverizer and cross 80 mesh sieves.
6. the preparation method preventing and treating the feed addictive of turbot fin rot according to claim 4, it is characterized in that: the preparation method of described earthworm powder is: the earthworm freeze drying cleaned up is ground on ice to finish-drying, after 50 mesh sieves, obtain earthworm powder.
7. the using method of preventing and treating the feed addictive of turbot fin rot according to any one of claim 1-3, is characterized in that: throw something and feed after being mixed with the weight ratio of 1:30-1:10 with commercially available basal feed by the feed addictive preventing and treating turbot fin rot of the present invention.
8. using method of preventing and treating the feed addictive of turbot fin rot according to claim 7, is characterized in that: the weight ratio of the feed addictive and commercially available basal feed of preventing and treating turbot fin rot of the present invention is 1:20.
CN201510973793.XA 2015-12-22 2015-12-22 Feed additive for preventing and treating turbot fin rot and preparation method thereof Withdrawn CN105410481A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107334001A (en) * 2017-07-20 2017-11-10 通化师范学院 A kind of Chinese herbal feed additive for preventing golden trout moth fin disease and application thereof

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Publication number Priority date Publication date Assignee Title
CN101744924A (en) * 2010-01-26 2010-06-23 中国水产科学研究院黄海水产研究所 Multiple-effect Chinese herbal medicine compound of bacterial diseases of turbot
CN102961660A (en) * 2012-12-14 2013-03-13 张�杰 Chinese herbal medicine for treating fish diseases
CN105029105A (en) * 2015-08-28 2015-11-11 青岛海之源智能技术有限公司 Low-cost and easy-to-eat mullet compound feed and manufacturing method thereof
CN105106893A (en) * 2015-09-15 2015-12-02 青岛海之源智能技术有限公司 Chinese herbal medicine feed additive for preventing white head-mouth disease of xiphophorus helleri and preparation method of Chinese herbal medicine feed additive

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Publication number Priority date Publication date Assignee Title
CN101744924A (en) * 2010-01-26 2010-06-23 中国水产科学研究院黄海水产研究所 Multiple-effect Chinese herbal medicine compound of bacterial diseases of turbot
CN102961660A (en) * 2012-12-14 2013-03-13 张�杰 Chinese herbal medicine for treating fish diseases
CN105029105A (en) * 2015-08-28 2015-11-11 青岛海之源智能技术有限公司 Low-cost and easy-to-eat mullet compound feed and manufacturing method thereof
CN105106893A (en) * 2015-09-15 2015-12-02 青岛海之源智能技术有限公司 Chinese herbal medicine feed additive for preventing white head-mouth disease of xiphophorus helleri and preparation method of Chinese herbal medicine feed additive

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107334001A (en) * 2017-07-20 2017-11-10 通化师范学院 A kind of Chinese herbal feed additive for preventing golden trout moth fin disease and application thereof

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Application publication date: 20160323