CN105288302A - Feed additive for preventing and treating fester disease of Epinephelus akaara and preparation method of feed additive - Google Patents
Feed additive for preventing and treating fester disease of Epinephelus akaara and preparation method of feed additive Download PDFInfo
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- CN105288302A CN105288302A CN201510853544.7A CN201510853544A CN105288302A CN 105288302 A CN105288302 A CN 105288302A CN 201510853544 A CN201510853544 A CN 201510853544A CN 105288302 A CN105288302 A CN 105288302A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention belongs to the technical field of aquiculture, relates to a feed additive for preventing and treating fester disease of Epinephelus akaara, and further relates to a preparation method of the feed additive for treating the fester disease of Epinephelus akaara. The feed additive comprises the following components in parts by mass: 36-44 parts of onion juice, 27-33 parts of a garden burnet infusion, 24-30 parts of gallnut, 24-30 parts of honeysuckle, 23-31 parts of phaseolus calcaratus, 23-31 parts of red bean, 21-29 parts of herba houttuyniae, 21-28 parts of radix arnebiae, 20-26 parts of mentha arvensis, 19-27 parts of oroxylum indicum, 17-25 parts of coriander, 17-24 parts of polygonum orientale, 16-24 parts of reed rhizome, 15-23 parts of milk vetch, 13-23 parts of euphorbia helioscopia, 13-20 parts of cicada ecdysis and the like. The feed additive can effectively prevent and treat the fester disease of Epinephelus akaara.
Description
Technical field
The invention belongs to technical field of aquaculture, particularly relate to a kind of feed additive preventing and treating epinephelus akaara ulcer disease; The invention still further relates to a kind of preparation method of feed additive of above-mentioned control epinephelus akaara ulcer disease.
Background technology
Epinephelus akaara (Epinephelusakaara) popular name is lithosporic, erythema, for one of them of spoke fin net-rope Perciformes perch Ya Mu Sushi section is planted, all distribution is had in subtropical zone, the whole world and tropical seas, its meat is fertile fresh and tender, nutritious, important marine fish, one of most important seawater cage culture high-level fish of Ye Shi China.Epinephelus akaara cultivation mainly concentrates on Asia, and the cultivation of external epinephelus akaara starts from the sixties in 20th century, domesticly starts from 20 century 70s, mainly concentrates on the provinces such as coastal Fujian, Guangdong, Zhejiang, Hainan, Guangxi, Taiwan.At present, the breeding way of epinephelus akaara has cage culture, pond culture and sea area enhancement releasing etc.In recent years, along with the popularization of the multiple aquaculture model of epinephelus akaara and the breakthrough of artificial propagation and seedling-raising technique, the rise of its aquaculture industry has greatly been promoted.
Ulcer disease is the common disease of epinephelus akaara, and its morbidity season is wide, and mortality rate is high, and morbidity season is April ~ November.Ill epinephelus akaara cardinal symptom is that action is blunt, and swim out of the water surface, each fin and base portion ulcer, even black and blue all over, ulcer surface differs in size, different, and along with the growth of sick time, the face of festering can be deepened, and finally dead, mortality rate is very high.The pathogenic bacterium of ulcer disease are Vibrio vulnificus.Therapeutic Method conventional at present embathes 3 minutes ~ 5 minutes with nitrofural, and (or) antibiotic for oral administration, with symptom management.Its shortcoming is that cure rate is low, and due to drug residue, the cabrilla after edible healing can affect the health of people, and abuse of antibiotics can make the drug resistance of antibacterial strengthen in addition, is unfavorable for the sustainable health development of aquaculture.At present, also someone develops the vaccine of cabrilla ulcer disease, and it utilizes in the formalin-inactivated vaccine of Vibrio vulnificus virulent strain vegetation, heat-inactivated vaccine and Vibrio vulnificus the rough lipopolysaccharide extracted, by intramuscular inoculation cabrilla and cabrilla, but its complex operation, cost is higher.Therefore, develop a kind of environmental protection, can the effective feed additive that occurs of the corrupt disease of prevention and therapy Ietalurus Punetaus hemorrhagic, there is important economic implications and market prospect.
Summary of the invention
An object of the present invention is to provide a kind of feed additive preventing and treating epinephelus akaara ulcer disease, its raw material components is green pure natural material, there is good bactericidal and bacteriostatic effect to the pathogen Vibrio vulnificus of epinephelus akaara ulcer disease, effectively can reduce the sickness rate of epinephelus akaara ulcer disease, the immunity improving epinephelus akaara and survival rate.
In order to solve the problems of the technologies described above, the present invention by the following technical solutions, a kind of feed additive preventing and treating epinephelus akaara ulcer disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 36-44 part, Radix Sanguisorbae infusion 27-33 part, Galla Chinensis 24-30 part, Flos Lonicerae 24-30 part, Semen Phaseoli 23-31 part, Semen Phaseoli 23-31 part, Herba Houttuyniae 21-29 part, Radix Arnebiae (Radix Lithospermi) 21-28 part, wild mint 20-26 part, Semen Oroxyli 19-27 part, coriander 17-25 part, smartweed 17-24 part, Rhizoma Phragmitis 16-24 part, Herba Astragali Melilotoidis (Herba Astragali Sinici) 15-23 part, Herba Euphorbiae Helioscopiae 13-23 part, Periostracum Cicadae 13-20 part, attached column flower 12-20 part, Radix Stephaniae Cepharanthae 10-20 part, Cortex Pseudolaricis 10-18 part, Zanthoxyli Bungeani powder 9-19 part, Herba Violae 9-19 part, Bulbus Allii 7-15 part, bamboo salt 8-14 part, clam worm powder 15-23 part, red line worm powder 15-23 part, Fructus Crataegi powder 13-19 part, fish oil 11-19 part.
Preferably, the feed additive of described control epinephelus akaara ulcer disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 39-41 part, Radix Sanguisorbae infusion 29-31 part, Galla Chinensis 26-28 part, Flos Lonicerae 26-28 part, Semen Phaseoli 26-28 part, Semen Phaseoli 26-28 part, Herba Houttuyniae 24-26 part, Radix Arnebiae (Radix Lithospermi) 24-25 part, wild mint 22-24 part, Semen Oroxyli 22-24 part, coriander 20-22 part, smartweed 19-21 part, Rhizoma Phragmitis 19-21 part, Herba Astragali Melilotoidis (Herba Astragali Sinici) 18-20 part, Herba Euphorbiae Helioscopiae 17-19 part, Periostracum Cicadae 15-18 part, attached column flower 15-17 part, Radix Stephaniae Cepharanthae 14-16 part, Cortex Pseudolaricis 13-15 part, Zanthoxyli Bungeani powder 13-16 part, Herba Violae 13-15 part, Bulbus Allii 10-12 part, bamboo salt 10-12 part, clam worm powder 18-20 part, red line worm powder 18-20 part, Fructus Crataegi powder 15-17 part, fish oil 14-16 part.
Preferably, the feed additive of described control epinephelus akaara ulcer disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 40 parts, Radix Sanguisorbae infusion 30 parts, Galla Chinensis 27 parts, Flos Lonicerae 27 parts, Semen Phaseoli 27 parts, 27 parts, Semen Phaseoli, Herba Houttuyniae 25 parts, Radix Arnebiae (Radix Lithospermi) 24 parts, wild mint 23 parts, Semen Oroxyli 23 parts, coriander 21 parts, smartweed 20 parts, Rhizoma Phragmitis 20 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 19 parts, Herba Euphorbiae Helioscopiae 18 parts, Periostracum Cicadae 16 parts, attached column spends 16 parts, Radix Stephaniae Cepharanthae 15 parts, Cortex Pseudolaricis 14 parts, Zanthoxyli Bungeani powder 14 parts, Herba Violae 14 parts, 11 parts, Bulbus Allii, bamboo salt 11 parts, clam worm powder 19 parts, 19 parts, red line worm powder, Fructus Crataegi powder 16 parts, 15 parts, fish oil.
Each component effect of the present invention is resolved as follows:
Nutritional labeling in Bulbus Allii Cepae is very abundant, is not only rich in potassium, vitamin C, folic acid, zinc, selenium, and the nutrient such as cellulosic, more has the nutrient substance that two kinds special: Quercetin and prostaglandin A; Phytocide is contained as garlicin etc. in Bulbus Allii Cepae, there is very strong sterilizing ability, can effectively resist influenza virus, preventing cold, when the bacteriocidin of Bulbus Allii Cepae is discharged via respiratory tract, urinary tract, sweat gland, the cell duct wall of these positions can be stimulated to secrete, thus eliminate the phlegm again, diuresis, diaphoresis and the antibacterial effect such as anticorrosion.Radix Sanguisorbae another name " yellow pawl is fragrant ", " beautiful letter ", " beautiful fermented soya beans, salted or other wise " or " acid is reddish brown " etc., cold in nature, bitter in the mouth acid, nontoxic; Return liver, lung, kidney and large intestine channel, have cooling blood for hemostasis, heat-clearing and toxic substances removing, train clear yin nourishing, the effects such as detumescence sore.Galla Chinensis is cold in nature, sour in the mouth, puckery, has the effects such as pathogenic fire reducing of astringing the lung, relieving diarrhea with astringents, arresting sweating hemostasis, removing dampness sore, cures mainly the diseases such as chronic cough of deficiency lung, chronic diarrhea chronic dysentery, hemorrhoidal bleeding of having blood in stool, carbuncle sore tumefacting virus, Skin wet and rotten.Flos Lonicerae contains the pharmacological component such as chlorogenic acid, luteolin glycosides, stronger restraint is had to the various pathogens such as Hemolytic streptococcus, staphylococcus aureus and upper respiratory tract infection Causative virus etc., in addition also can enhancing immunity, antiearly pregnancy, hepatoprotective, antitumor, antiinflammatory, antipyretic, hemostasis (blood coagulation), suppress intestinal absorption cholesterol etc., its clinical application widely, can be used for the treatment of respiratory tract infection, bacillary dysentery, acute urinary, hypertension etc. more than 40 with other medicines compatibility and plant disease.Semen Phaseoli is put down, sweet in the mouth, acid, can inducing diuresis and reducing edema (jaundice, dysentery, has blood in stool, carbuncle for edema, beriberi), heat clearing away jaundice eliminating, removing toxic substances and promoting pus discharge.Semen Phaseoli can anti-inflammation, swollen sitting and lying of harnessing the river must not, women's head-ornaments health know swollen, control perianal abscess defecate normal blood, control hematochezia due to toxic heat, control cellulitis maiden work, Semen Phaseoli energy pain relieving dispel itch is subsided a swelling.Herba Houttuyniae has heat-clearing and toxic substances removing, eliminating carbuncle evacuation of pus, the effect of inducing diuresis for treating stranguria syndrome.Radix Arnebiae (Radix Lithospermi) bitter in the mouth cold in nature, can removing heat from blood, invigorates blood circulation, heat clearing away, removing toxic substances, controls warm macule, jaundice due to damp-heat, purpura, tells, is defeated in battle, hematuria, stranguria with turbid discharge, constipation with heat retention, burn, eczema, erysipelas, carbuncle and ulcer.Wild mint acrid in the mouth, cool in nature, can inducing diaphoresis to dispel wind.Seed, the peel of stem of Semen Oroxyli contain baicaligenin, have antiinflammatory, antiallergic action, diuresis, function of gallbladder promoting, the effect of cholesterol reducing, contain chrysin, have cytotoxic activity to human nasal pharyngeal cancer (KB) cell in seed and peel of stem.The pungent taste temperature of coriander, have diaphoresis rash, removing stagnancy of indigested food and making QI downward, be amusing and in effect, curing mainly the measles initial stage appears the diseases such as smooth, food stagnant, appetite is not opened, proctoptosis.Smartweed acrid in the mouth, property is put down, Gui Jing, liver, energy expelling wind and removing dampness; Heat-clearing and toxic substances removing; Invigorate blood circulation; Preventing the attack (or recurrence) of malaria, cures mainly rheumatic arthralgia; Dysentery; Diarrhoea; Vomit and diarrhoea and have a cramp; Edema; Foot gas; Carbuncle skin ulcer furuncle furuncle; Snake bite and insect sting; Infantile malnutrition hernia; Traumatic injury; Malaria, can except malignant boil swell, control hernia, can antitumor, antibacterial, diuresis.Rhizoma Phragmitis sweet in the mouth, cold in nature, return lung meridian, stomach warp, for calentura excessive thirst, gastropyretic vomiting, cough due to lung-heat, lung abscess vomiting pus, the puckery pain of pyretic stranguria.Herba Astragali Melilotoidis (Herba Astragali Sinici) cures mainly removing blood stasis, hemostasia and promoting granulation, and profit can support the five internal organs, improve visual and auditory acuity, hot blast of dispelling, makes us spry and light, and food is not minded for a long time, mends people, can stop hot malaria, flat stomach of invigorating blood circulation.Herba Euphorbiae Helioscopiae acrid in the mouth, hardship, cold nature, line water detumescence, preventing phlegm from forming and stopping coughing, removing toxic substances parasite killing, cures mainly edematous asthma, and phlegm retention is breathed with cough malaria, bacillary dysentery, scrofula, Tuberculous fistula, osteomyelitis.Periostracum Cicadae energy dispelling wind-heat, rash sore-throat relieving, removing nebula to improve visual acuity, to expelling wind and relieving convulsion.Attached column flower mildly bitter flavor, pungent, cool in nature, can heat-clearing and toxic substances removing; Dampness removing is antipruritic; Reducing swelling and alleviating pain, main dysentery; Skin infection; Eczema; Skin pruritus; Caterpillar is stabbed; Traumatic injury.Radix Stephaniae Cepharanthae is Menispermaceae stephania plant, is used as medicine with tuber, can heat-clearing and toxic substances removing, cooling blood for hemostasis, and dissipating blood stasis for subsidence of swelling, can be used for acute hepatitis, bacillary dysentery, acute appendicitis, stomachache, internal hemorrhage, traumatic injury, venom; Mumps is controlled in external, lymphadenitis, neurodermatitis.Cortex Pseudolaricis is again Cortex Pseudolaricis, pseudolaricis,cortex, and have the effect that significant anti-tumor neovascularization is formed, antitumor action, antifertility action, blood vessel formation against function, antifungic action, Pseudolarix acid B can peroxide activator proliferator-activated receptor.Pericarpium Zanthoxyli acrid in the mouth, hot in nature, return spleen, stomach warp; Have warming spleen and stomach for dispelling cold, removing damp and stopping pain, parasite killing detoxifies, effect of antipruritic solution raw meat; Primary treatment is vomitted, and anemofrigid-damp arthralgia, the diseases such as toothache, taking pepper water can drive away parasite.Herba Violae taste bitter, acrid, cold, GUIXIN, lung meridian, have heat-clearing and toxic substances removing, and removing heat from blood is subsided a swelling, and the effect of clearing away heat-damp and promoting diuresis, cures mainly furuncle, carbuncle, scrofula, jaundice, dysentery, diarrhoea, conjunctival congestion, sore throat, venom.Bulbus Allii removing toxic substances parasite killing, reducing swelling and alleviating pain, antidiarrheal dysentery relieving, controls lung, anthelmintic, and also have warming the spleen warming the stomach in addition, control swollen ulcer drug, white bald tinea skin ulcer, dysentery is had loose bowels, pulmonary tuberculosis pertussis, ascarid pinworm, eating accumulation, coldness and pain in the epigastrium, edema distension.Bamboo salt has antibacterial, antiinflammatory, toxopexic effect.Clam worm clam worm delicious flavour, nutritious, the multiple nutritional components such as its rich in proteins and calcium, phosphorus, ferrum, iodine, and there is higher medicinal and food therapy value, property is salty-cold, has nourishing YIN to lower pathogenic fire, the function of tonify deficiency lung heat clearing has blood pressure lowering, controls wet, protective effect on cancer risk, clam worm is cold in nature, sweet in the mouth, salty, has effect of nourishing YIN to lower pathogenic fire, lung heat clearing tonify deficiency.Red line worm can be used as the high-quality animal feed of aquatic animal, can reduce the production cost of feedstuff, improves culture benefit and forms quality.Fructus Crataegi can improve mouthfeel, and can spleen benefiting and stimulating the appetite, relieving dyspepsia, phlegm reduction of blood circulation promoting, antiinflammatory antibacterial.Fish oil can not only provide energy for epinephelus akaara and have food calling effect.
Another object of the present invention is to provide a kind of preparation method of feed additive of above-mentioned control epinephelus akaara ulcer disease, and the preparation method of the feed additive of this control epinephelus akaara ulcer disease comprises the following steps:
(1) by Bulbus Allii Cepae grinding or put into juice extractor and squeeze the juice, Bulbus Allii Cepae juice is obtained;
(2) cleaned by Radix Sanguisorbae, drain the water, add the purified water that parts by weight are 4 times of Radix Sanguisorbae parts by weight, 70-80 DEG C is soaked 4-6h, filters to obtain the infusion of Radix Sanguisorbae;
(3) take Galla Chinensis, Flos Lonicerae, Herba Houttuyniae, Radix Arnebiae (Radix Lithospermi), wild mint, Semen Oroxyli, coriander, smartweed and Rhizoma Phragmitis according to proportioning, gross weight is designated as 1 part, adds 6 parts of purified water, and 70-80 DEG C is soaked 6-9h, obtain soak;
(4) boil 2h after the soak that step (3) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 4 parts in medical material, continue to boil 1h after being heated to boiling, merge twice fried liquid and be extracting solution;
(5) take Semen Phaseoli, Semen Phaseoli, Herba Astragali Melilotoidis (Herba Astragali Sinici), Herba Euphorbiae Helioscopiae, Periostracum Cicadae, attached column flower, Radix Stephaniae Cepharanthae, Cortex Pseudolaricis, Zanthoxyli Bungeani powder, Herba Violae and Bulbus Allii respectively according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(6) grounds travel that extracting solution step (4) obtained and step (5) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(7) take Bulbus Allii Cepae juice, the infusion of Radix Sanguisorbae, bamboo salt, clam worm powder, red line worm powder, Fructus Crataegi powder and fish oil respectively according to proportioning, mix homogeneously with the extract of step (6) gained, obtain object.
Further, the preparation method of described clam worm powder is: the clam worm lyophilization cleaned up ground on ice to finish-drying, after 50 mesh sieves, obtain clam worm powder.Cryodesiccated advantage can not make the active substance inactivation contained by clam worm in dry run, because constantly stir produces heat, to keep temperature, protective tissue when the advantage of grinding is and reduces grinding on ice.
Further, the preparation method of described red line worm powder is: grind after the red line worm lyophilization to finish-drying cleaned up on ice, and after 50 mesh sieves, obtains red line worm powder.Cryodesiccated advantage is; The active substance inactivation contained by red line worm can not be made in dry run, when the advantage of grinding is and reduces grinding, produce heat because constantly stirring, to keep temperature, protective tissue on ice.
Usage and dosage: throw something and feed after the feed additive of control epinephelus akaara ulcer disease of the present invention is mixed homogeneously with the weight ratio of 1:30-1:10 with commercially available normal feedstuff, preferably, the feed additive of control epinephelus akaara ulcer disease of the present invention and the weight ratio of commercially available normal feedstuff are 1:20.
In preparation method, crude drug purified water soaking a period of time, medical material deliquescing, cell expansion, effective ingredient is easily fried, and medical material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medical material surface, protein coagulating, the capillary channel on blocking medical material surface, make the moisture when decocting enter not go, effective ingredient is not easily fried, affects curative effect of medication; Decoction can change the irritation effect of medicine, relatively mildly acts on cabrilla, promotes that cabrilla is to the absorption of medicine; Grinding makes the starch in medical material, protein is separated out more fully at subsequent step; Supercritical extraction, immersion, concentration step retain the effective ingredient of each component in the present invention to greatest extent; Ground or put into juice extractor by Bulbus Allii Cepae and squeeze the juice, the advantage of getting gained juice is that preparation method is simple, saves medicinal raw material, improves the utilization rate to Bulbus Allii Cepae, and take full advantage of the effective ingredient of Bulbus Allii Cepae, the better effects if of bactericidal; Cleaned by Radix Sanguisorbae after draining the water and add the purified water that parts by weight are 4 times of Radix Sanguisorbae parts by weight, then 70-80 DEG C is soaked 4-6h, gets the infusion of Radix Sanguisorbae, its objective is the effective ingredient of fully separating out in Radix Sanguisorbae.
Because the fish oil contained in feed additive of the present invention has food calling effect, red line worm and clam worm delicious flavour, Fructus Crataegi can improve the overall mouthfeel of feedstuff and energy spleen benefiting and stimulating the appetite, relieving dyspepsia, therefore the feed additive of control epinephelus akaara ulcer disease of the present invention can improve the mouthfeel of normal feedstuff, stimulate the taste bud of epinephelus akaara, induction epinephelus akaara ingests and adds the normal feedstuff of feed additive of the present invention, therefore epinephelus akaara immunity and resistance can be strengthened in feed additive of the present invention, the composition pathogen Vibrio vulnificus of ulcer disease to remarkable bactericidal and bacteriostatic effect can enter in epinephelus akaara body, and play it and act on accordingly.
The feed additive of control epinephelus akaara ulcer disease of the present invention is the etiopathogenesis according to ulcer disease, the suitable component of selection, and is organically combined, and has significant cooperative synergism effect, plays the function of each component to greatest extent.The component of the feed additive of control epinephelus akaara ulcer disease of the present invention is environmental protection raw material, nontoxic to environment, not containing chemical drugs or antibiotic, can not affect the healthy of people after edible cabrilla of having cured, and feed additive of the present invention is good to the curative effect of cabrilla ulcer disease, cure rate is high, make the survival rate of ill cabrilla to more than 90%, after medication 2-4 days, cabrilla starts progressively to recover to ingest, and ingestion frequency improves.
The present invention uses safer, little to cabrilla damage, after using the feed additive for the treatment of cabrilla ulcer disease of the present invention; cabrilla Health restoration is fast, medication 3-10 days, and cabrilla can recover normal activity; idle fund is strong and vigorous, and wound progressively heals, and ingests completely normal.And the feed additive of control epinephelus akaara ulcer disease of the present invention effectively can also prevent the generation of cabrilla ulcer disease.
Compared with prior art, the invention has the beneficial effects as follows:
(1) fish oil owing to containing in feed additive of the present invention has food calling effect, red line worm and clam worm delicious flavour, Fructus Crataegi can improve the overall mouthfeel of feedstuff and energy spleen benefiting and stimulating the appetite, relieving dyspepsia, therefore the feed additive of control epinephelus akaara ulcer disease of the present invention can improve the mouthfeel of normal feedstuff, stimulate the taste bud of epinephelus akaara, induction epinephelus akaara ingests and adds the normal feedstuff of feed additive of the present invention, therefore epinephelus akaara immunity and resistance can be strengthened in feed additive of the present invention, the composition pathogen point-like Vibrio vulnificus of ulcer disease to remarkable bactericidal and bacteriostatic effect can enter in epinephelus akaara body, and play it and act on accordingly.
(2) feed additive of control epinephelus akaara ulcer disease of the present invention, is the Chinese herbal medicine selected according to the etiopathogenesis of ulcer disease, and is organically combined, have significant cooperative synergism effect, played the function of each component to greatest extent.
(3) pathogen of feed additive to cabrilla ulcer disease of control epinephelus akaara ulcer disease of the present invention has good inhibition.
(4) component of the feed additive of control epinephelus akaara ulcer disease of the present invention is environmental protection raw material, nontoxic to environment, without chemical drugs or antibiotic, the healthy of people can not be affected after edible cabrilla of having cured, and the feed additive of control epinephelus akaara ulcer disease of the present invention is very little to cabrilla damage, after using the feed additive of control epinephelus akaara ulcer disease of the present invention, cabrilla Health restoration is fast.In addition, the feed additive of control epinephelus akaara ulcer disease of the present invention is easy to use, will not pull rear medicine-feeding out by sick fish, decrease the injury to sick fish in operating process.
(5) in preparation method, crude drug purified water soaking a period of time, medical material deliquescing, cell expansion, effective ingredient is easily fried, and medical material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medical material surface, protein coagulating, the capillary channel on blocking medical material surface, make the moisture when decocting enter not go, effective ingredient is not easily fried, affects curative effect of medication; Decoction can change the irritation effect of medicine, relatively mildly acts on cabrilla, promotes that cabrilla is to the absorption of medicine; Grinding makes the starch in medical material, protein is separated out more fully at subsequent step; Supercritical extraction, immersion, concentration step retain the effective ingredient in Chinese medicine of the present invention to greatest extent; Ground or put into juice extractor by Bulbus Allii Cepae and squeeze the juice, the advantage of getting gained juice is that preparation method is simple, saves medicinal raw material, improves the utilization rate to Bulbus Allii Cepae, and take full advantage of the effective ingredient of Bulbus Allii Cepae, the better effects if of bactericidal; Cleaned by Radix Sanguisorbae after draining the water and add the purified water that parts by weight are 4 times of Radix Sanguisorbae parts by weight, then 70-80 DEG C is soaked 4-6h, gets the infusion of Radix Sanguisorbae, its objective is the effective ingredient of fully separating out in Radix Sanguisorbae.
Detailed description of the invention
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.
Embodiment 1
A kind of feed additive preventing and treating epinephelus akaara ulcer disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 41 parts, Radix Sanguisorbae infusion 31 parts, Galla Chinensis 30 parts, Flos Lonicerae 28 parts, Semen Phaseoli 28 parts, 28 parts, Semen Phaseoli, Herba Houttuyniae 27 parts, Radix Arnebiae (Radix Lithospermi) 25 parts, wild mint 24 parts, Semen Oroxyli 24 parts, coriander 22 parts, smartweed 21 parts, Rhizoma Phragmitis 21 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 20 parts, Herba Euphorbiae Helioscopiae 19 parts, Periostracum Cicadae 18 parts, attached column spends 17 parts, Radix Stephaniae Cepharanthae 17 parts, Cortex Pseudolaricis 18 parts, Zanthoxyli Bungeani powder 16 parts, Herba Violae 15 parts, 12 parts, Bulbus Allii, bamboo salt 12 parts, clam worm powder 23 parts, 23 parts, red line worm powder, Fructus Crataegi powder 18 parts, 19 parts, fish oil.
The preparation method of the feed additive of above-mentioned control epinephelus akaara ulcer disease comprises the following steps:
(1) by Bulbus Allii Cepae grinding or put into juice extractor and squeeze the juice, Bulbus Allii Cepae juice is obtained;
(2) cleaned by Radix Sanguisorbae, drain the water, add the purified water that parts by weight are 4 times of Radix Sanguisorbae parts by weight, 70-80 DEG C is soaked 4-6h, filters to obtain the infusion of Radix Sanguisorbae;
(3) take Galla Chinensis, Flos Lonicerae, Herba Houttuyniae, Radix Arnebiae (Radix Lithospermi), wild mint, Semen Oroxyli, coriander, smartweed and Rhizoma Phragmitis according to proportioning, gross weight is designated as 1 part, adds 6 parts of purified water, and 70-80 DEG C is soaked 6-9h, obtain soak;
(4) boil 2h after the soak that step (3) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 4 parts in medical material, continue to boil 1h after being heated to boiling, merge twice fried liquid and be extracting solution;
(5) take Semen Phaseoli, Semen Phaseoli, Herba Astragali Melilotoidis (Herba Astragali Sinici), Herba Euphorbiae Helioscopiae, Periostracum Cicadae, attached column flower, Radix Stephaniae Cepharanthae, Cortex Pseudolaricis, Zanthoxyli Bungeani powder, Herba Violae and Bulbus Allii respectively according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(6) grounds travel that extracting solution step (4) obtained and step (5) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(7) take Bulbus Allii Cepae juice, the infusion of Radix Sanguisorbae, bamboo salt, clam worm powder, red line worm powder, Fructus Crataegi powder and fish oil respectively according to proportioning, mix homogeneously with the extract of step (6) gained, obtain object.
The preparation method of described clam worm powder is: the clam worm lyophilization cleaned up ground on ice to finish-drying, after 50 mesh sieves, obtain clam worm powder.
The preparation method of described red line worm powder is: grind after the red line worm lyophilization to finish-drying cleaned up on ice, and after 50 mesh sieves, obtains red line worm powder.
Embodiment 2
A kind of feed additive preventing and treating epinephelus akaara ulcer disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 44 parts, Radix Sanguisorbae infusion 29 parts, Galla Chinensis 28 parts, Flos Lonicerae 30 parts, Semen Phaseoli 30 parts, 30 parts, Semen Phaseoli, Herba Houttuyniae 26 parts, Radix Arnebiae (Radix Lithospermi) 28 parts, wild mint 26 parts, Semen Oroxyli 27 parts, coriander 25 parts, smartweed 24 parts, Rhizoma Phragmitis 24 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 23 parts, Herba Euphorbiae Helioscopiae 23 parts, Periostracum Cicadae 20 parts, attached column spends 20 parts, Radix Stephaniae Cepharanthae 16 parts, Cortex Pseudolaricis 15 parts, Zanthoxyli Bungeani powder 13 parts, Herba Violae 13 parts, 15 parts, Bulbus Allii, bamboo salt 14 parts, clam worm powder 21 parts, 20 parts, red line worm powder, Fructus Crataegi powder 19 parts, 19 parts, fish oil.
The preparation method of the feed additive of the control epinephelus akaara ulcer disease of the present embodiment is with embodiment 1.
Embodiment 3
A kind of feed additive preventing and treating epinephelus akaara ulcer disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 39 parts, Radix Sanguisorbae infusion 33 parts, Galla Chinensis 30 parts, Flos Lonicerae 30 parts, Semen Phaseoli 31 parts, 31 parts, Semen Phaseoli, Herba Houttuyniae 29 parts, Radix Arnebiae (Radix Lithospermi) 24 parts, wild mint 25 parts, Semen Oroxyli 25 parts, coriander 20 parts, smartweed 19 parts, Rhizoma Phragmitis 19 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 20 parts, Herba Euphorbiae Helioscopiae 19 parts, Periostracum Cicadae 15 parts, attached column spends 15 parts, Radix Stephaniae Cepharanthae 20 parts, Cortex Pseudolaricis 18 parts, Zanthoxyli Bungeani powder 19 parts, Herba Violae 19 parts, 10 parts, Bulbus Allii, bamboo salt 8 parts, clam worm powder 20 parts, 18 parts, red line worm powder, Fructus Crataegi powder 17 parts, 16 parts, fish oil.
The preparation method of the feed additive of the control epinephelus akaara ulcer disease of the present embodiment is with embodiment 1.
Embodiment 4
A kind of feed additive preventing and treating epinephelus akaara ulcer disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 36 parts, Radix Sanguisorbae infusion 27 parts, Galla Chinensis 26 parts, Flos Lonicerae 26 parts, Semen Phaseoli 26 parts, 26 parts, Semen Phaseoli, Herba Houttuyniae 24 parts, Radix Arnebiae (Radix Lithospermi) 21 parts, wild mint 22 parts, Semen Oroxyli 22 parts, coriander 17 parts, smartweed 19 parts, Rhizoma Phragmitis 16 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 18 parts, Herba Euphorbiae Helioscopiae 17 parts, Periostracum Cicadae 13 parts, attached column spends 12 parts, Radix Stephaniae Cepharanthae 14 parts, Cortex Pseudolaricis 13 parts, Zanthoxyli Bungeani powder 9 parts, Herba Violae 9 parts, 7 parts, Bulbus Allii, bamboo salt 10 parts, clam worm powder 18 parts, 18 parts, red line worm powder, Fructus Crataegi powder 15 parts, 14 parts, fish oil.
The preparation method of the feed additive of the control epinephelus akaara ulcer disease of the present embodiment is with embodiment 1.
Embodiment 5
A kind of feed additive preventing and treating epinephelus akaara ulcer disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 40 parts, Radix Sanguisorbae infusion 31 parts, Galla Chinensis 24 parts, Flos Lonicerae 24-30 part, Semen Phaseoli 23 parts, 23 parts, Semen Phaseoli, Herba Houttuyniae 21 parts, Radix Arnebiae (Radix Lithospermi) 22 parts, wild mint 20 parts, Semen Oroxyli 19 parts, coriander 22 parts, smartweed 17 parts, Rhizoma Phragmitis 19 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 15 parts, Herba Euphorbiae Helioscopiae 13 parts, Periostracum Cicadae 13 parts, attached column spends 15 parts, Radix Stephaniae Cepharanthae 10 parts, Cortex Pseudolaricis 10 parts, Zanthoxyli Bungeani powder 13 parts, Herba Violae 13 parts, 10 parts, Bulbus Allii, bamboo salt 12 parts, clam worm powder 15 parts, 15 parts, red line worm powder, Fructus Crataegi powder 13 parts, 11 parts, fish oil.
The preparation method of the feed additive of the control epinephelus akaara ulcer disease of the present embodiment is with embodiment 1.
Embodiment 6
A kind of feed additive preventing and treating epinephelus akaara ulcer disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 40 parts, Radix Sanguisorbae infusion 30 parts, Galla Chinensis 27 parts, Flos Lonicerae 27 parts, Semen Phaseoli 27 parts, 27 parts, Semen Phaseoli, Herba Houttuyniae 25 parts, Radix Arnebiae (Radix Lithospermi) 24 parts, wild mint 23 parts, Semen Oroxyli 23 parts, coriander 21 parts, smartweed 20 parts, Rhizoma Phragmitis 20 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 19 parts, Herba Euphorbiae Helioscopiae 18 parts, Periostracum Cicadae 16 parts, attached column spends 16 parts, Radix Stephaniae Cepharanthae 15 parts, Cortex Pseudolaricis 14 parts, Zanthoxyli Bungeani powder 14 parts, Herba Violae 14 parts, 11 parts, Bulbus Allii, bamboo salt 11 parts, clam worm powder 19 parts, 19 parts, red line worm powder, Fructus Crataegi powder 16 parts, 15 parts, fish oil.
The preparation method of the feed additive of the control epinephelus akaara ulcer disease of the present embodiment is with embodiment 1.
Experimental result
Contrast experiment 1
Test site is selected in fishing ground, Qingdao, in order to verify therapeutic effect of the present invention, is testing the cabrilla suffering from ulcer disease:
The process of matched group is as follows: every day, ill cabrilla was embathed 3 minutes ~ 5 minutes by the nitrofural of 2ppm, until the recovery from illness of sick fish.
The process of experiment A group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the feed additive of the control epinephelus akaara ulcer disease of the embodiment of the present invention 1.
The process of experiment B group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the feed additive of the control epinephelus akaara ulcer disease of the embodiment of the present invention 2.
The process of experiment C group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the feed additive of the control epinephelus akaara ulcer disease of the embodiment of the present invention 3.
The process of experiment D group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the feed additive of the control epinephelus akaara ulcer disease of the embodiment of the present invention 4.
The process of experiment E group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the feed additive of the control epinephelus akaara ulcer disease of the embodiment of the present invention 5.
The process of experiment F group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the feed additive of the control epinephelus akaara ulcer disease of the embodiment of the present invention 6.
Experimental result is in table 1:
The feed additive therapeutic effect of table 1 control of the present invention epinephelus akaara ulcer disease
Experimental result shows: the feed additive of control epinephelus akaara ulcer disease of the present invention, the etiopathogenesis according to ulcer disease, the suitable component of selection, and organically combined, there is significant cooperative synergism effect, play the function of each component to greatest extent.The component of the feed additive of control epinephelus akaara ulcer disease of the present invention is environmental protection raw material, nontoxic to environment, not containing chemical drugs or antibiotic, can not affect the healthy of people after edible cabrilla of having cured, and feed additive of the present invention is good to the curative effect of cabrilla ulcer disease, cure rate is high, make the survival rate of ill cabrilla to more than 90%, after medication 2-4 days, cabrilla starts progressively to recover to ingest, and ingestion frequency improves.In addition, the present invention uses safer, little to cabrilla damage, after using the feed additive for the treatment of cabrilla ulcer disease of the present invention; cabrilla Health restoration is fast, medication 3-10 days, and cabrilla can recover normal activity; idle fund is strong and vigorous, and wound progressively heals, and ingests completely normal.
Contrast experiment 2
With the processed group of normal feedstuff of throwing something and feeding for blank group.Experimental group process is as follows:
Experiment a group: throw something and feed after the feed additive of the control epinephelus akaara ulcer disease of the embodiment of the present invention 1 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
Experiment b group: throw something and feed after the feed additive of the control epinephelus akaara ulcer disease of the embodiment of the present invention 2 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
Experiment c group: throw something and feed after the feed additive of the control epinephelus akaara ulcer disease of the embodiment of the present invention 3 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
Experiment d group: throw something and feed after the feed additive of the control epinephelus akaara ulcer disease of the embodiment of the present invention 4 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
Experiment e group: throw something and feed after the feed additive of the control epinephelus akaara ulcer disease of the embodiment of the present invention 5 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
Experiment f group: throw something and feed after the feed additive of the control epinephelus akaara ulcer disease of the embodiment of the present invention 6 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
When experiment starts, each processed group puts into healthy cabrilla 10, after raising 15 days, adds the cabrilla that 1 is suffered from ulcer disease in each processed group by above-mentioned process, observes and records infection state after one week.The results are shown in Table 2:
The effect of the feed additive prevention ulcer disease of table 2 control of the present invention epinephelus akaara ulcer disease
From table 2, the feed additive of control epinephelus akaara ulcer disease of the present invention effectively can reduce the incidence rate of cabrilla ulcer disease, can improve the immunity of cabrilla, has good preventive effect to ulcer disease.
Bacteriostatic test checks
With nitrofural as matched group, the feed additive of the control epinephelus akaara ulcer disease of embodiment of the present invention 1-embodiment 6 is that experimental group carries out antibacterial inspection.
Culture medium: CC agar culture medium.
Strain is: Vibrio vulnificus.
Matched group antibacterial liquid is prepared: be dissolved in by nitrofural in sterile deionized water, concentration is 2ppm.
Experimental group antibacterial liquid is prepared: be dissolved in respectively in sterile deionized water by the feed additive of the control epinephelus akaara ulcer disease of embodiment of the present invention 1-embodiment 6, liquor strength being diluted to concentration is 2ppm.
Observe and record experimental result:
Represent with "-" without inhibition zone;
Antibacterial circle diameter < 10mm is slightly antibacterial, represents with "+";
Antibacterial circle diameter 10 ~ 14mm is that moderate is antibacterial, represents with " ++ ";
Antibacterial circle diameter > 14mm is highly antibacterial, represents with " +++ "; To the fungistatic effect of Vibrio vulnificus in table 3:
The bactericidal and bacteriostatic effect of the feed additive of table 3 control of the present invention epinephelus akaara ulcer disease
From table 3, the pathogen Vibrio vulnificus of feed additive to ulcer disease of control epinephelus akaara ulcer disease of the present invention has good bactericidal and bacteriostatic effect.
The above is only the preferred embodiment of the present invention, is noted that for those skilled in the art, and under the premise without departing from the principles of the invention, can also make some improvement, these improvement should be considered as protection scope of the present invention.
Claims (8)
1. prevent and treat the feed additive of epinephelus akaara ulcer disease for one kind, it is characterized in that: be made up of each component of following masses number: Bulbus Allii Cepae juice 36-44 part, Radix Sanguisorbae infusion 27-33 part, Galla Chinensis 24-30 part, Flos Lonicerae 24-30 part, Semen Phaseoli 23-31 part, Semen Phaseoli 23-31 part, Herba Houttuyniae 21-29 part, Radix Arnebiae (Radix Lithospermi) 21-28 part, wild mint 20-26 part, Semen Oroxyli 19-27 part, coriander 17-25 part, smartweed 17-24 part, Rhizoma Phragmitis 16-24 part, Herba Astragali Melilotoidis (Herba Astragali Sinici) 15-23 part, Herba Euphorbiae Helioscopiae 13-23 part, Periostracum Cicadae 13-20 part, attached column flower 12-20 part, Radix Stephaniae Cepharanthae 10-20 part, Cortex Pseudolaricis 10-18 part, Zanthoxyli Bungeani powder 9-19 part, Herba Violae 9-19 part, Bulbus Allii 7-15 part, bamboo salt 8-14 part, clam worm powder 15-23 part, red line worm powder 15-23 part, Fructus Crataegi powder 13-19 part, fish oil 11-19 part.
2. the feed additive of control epinephelus akaara ulcer disease according to claim 1, it is characterized in that: the feed additive of described control epinephelus akaara ulcer disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 39-41 part, Radix Sanguisorbae infusion 29-31 part, Galla Chinensis 26-28 part, Flos Lonicerae 26-28 part, Semen Phaseoli 26-28 part, Semen Phaseoli 26-28 part, Herba Houttuyniae 24-26 part, Radix Arnebiae (Radix Lithospermi) 24-25 part, wild mint 22-24 part, Semen Oroxyli 22-24 part, coriander 20-22 part, smartweed 19-21 part, Rhizoma Phragmitis 19-21 part, Herba Astragali Melilotoidis (Herba Astragali Sinici) 18-20 part, Herba Euphorbiae Helioscopiae 17-19 part, Periostracum Cicadae 15-18 part, attached column flower 15-17 part, Radix Stephaniae Cepharanthae 14-16 part, Cortex Pseudolaricis 13-15 part, Zanthoxyli Bungeani powder 13-16 part, Herba Violae 13-15 part, Bulbus Allii 10-12 part, bamboo salt 10-12 part, clam worm powder 18-20 part, red line worm powder 18-20 part, Fructus Crataegi powder 15-17 part, fish oil 14-16 part.
3. the feed additive of control epinephelus akaara ulcer disease according to claim 1, it is characterized in that: the feed additive of described control epinephelus akaara ulcer disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 40 parts, Radix Sanguisorbae infusion 30 parts, Galla Chinensis 27 parts, Flos Lonicerae 27 parts, Semen Phaseoli 27 parts, 27 parts, Semen Phaseoli, Herba Houttuyniae 25 parts, Radix Arnebiae (Radix Lithospermi) 24 parts, wild mint 23 parts, Semen Oroxyli 23 parts, coriander 21 parts, smartweed 20 parts, Rhizoma Phragmitis 20 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 19 parts, Herba Euphorbiae Helioscopiae 18 parts, Periostracum Cicadae 16 parts, attached column spends 16 parts, Radix Stephaniae Cepharanthae 15 parts, Cortex Pseudolaricis 14 parts, Zanthoxyli Bungeani powder 14 parts, Herba Violae 14 parts, 11 parts, Bulbus Allii, bamboo salt 11 parts, clam worm powder 19 parts, 19 parts, red line worm powder, Fructus Crataegi powder 16 parts, 15 parts, fish oil.
4. a preparation method for the feed additive of the control epinephelus akaara ulcer disease according to any one of claim 1-3, is characterized in that: comprise the following steps:
(1) by Bulbus Allii Cepae grinding or put into juice extractor and squeeze the juice, Bulbus Allii Cepae juice is obtained;
(2) cleaned by Radix Sanguisorbae, drain the water, add the purified water that parts by weight are 4 times of Radix Sanguisorbae parts by weight, 70-80 DEG C is soaked 4-6h, filters to obtain the infusion of Radix Sanguisorbae;
(3) take Galla Chinensis, Flos Lonicerae, Herba Houttuyniae, Radix Arnebiae (Radix Lithospermi), wild mint, Semen Oroxyli, coriander, smartweed and Rhizoma Phragmitis according to proportioning, gross weight is designated as 1 part, adds 6 parts of purified water, and 70-80 DEG C is soaked 6-9h, obtain soak;
(4) boil 2h after the soak that step (3) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 4 parts in medical material, continue to boil 1h after being heated to boiling, merge twice fried liquid and be extracting solution;
(5) take Semen Phaseoli, Semen Phaseoli, Herba Astragali Melilotoidis (Herba Astragali Sinici), Herba Euphorbiae Helioscopiae, Periostracum Cicadae, attached column flower, Radix Stephaniae Cepharanthae, Cortex Pseudolaricis, Zanthoxyli Bungeani powder, Herba Violae and Bulbus Allii respectively according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(6) grounds travel that extracting solution step (4) obtained and step (5) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(7) take Bulbus Allii Cepae juice, the infusion of Radix Sanguisorbae, bamboo salt, clam worm powder, red line worm powder, Fructus Crataegi powder and fish oil respectively according to proportioning, mix homogeneously with the extract of step (6) gained, obtain object.
5. the preparation method of the feed additive of control epinephelus akaara ulcer disease according to claim 4, it is characterized in that: the preparation method of described clam worm powder is: the clam worm lyophilization cleaned up is ground on ice to finish-drying, after 50 mesh sieves, obtain clam worm powder.
6. the preparation method of the feed additive of control epinephelus akaara ulcer disease according to claim 4, it is characterized in that: the preparation method of described red line worm powder is: grind after the red line worm lyophilization to finish-drying cleaned up on ice, after 50 mesh sieves, obtain red line worm powder.
7. a using method for the feed additive of the control epinephelus akaara ulcer disease according to any one of claim 1-3, is characterized in that: throw something and feed after being mixed homogeneously with the weight ratio of 1:30-1:10 with commercially available normal feedstuff by the feed additive of control epinephelus akaara ulcer disease of the present invention.
8. the using method of the feed additive of control epinephelus akaara ulcer disease according to claim 7, is characterized in that: the feed additive of control epinephelus akaara ulcer disease of the present invention and the weight ratio of commercially available normal feedstuff are 1:20.
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| CN113826781A (en) * | 2021-10-15 | 2021-12-24 | 永辰水产科技(盐城)有限公司 | Feed additive for preventing and treating largemouth black bass ulcer syndrome |
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