CN105360031B - A kind of method of quick foundation hybridization XY ♂ all-male Pelteobagrus fulvidracos - Google Patents
A kind of method of quick foundation hybridization XY ♂ all-male Pelteobagrus fulvidracos Download PDFInfo
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- CN105360031B CN105360031B CN201510818624.9A CN201510818624A CN105360031B CN 105360031 B CN105360031 B CN 105360031B CN 201510818624 A CN201510818624 A CN 201510818624A CN 105360031 B CN105360031 B CN 105360031B
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- 241000376029 Tachysurus fulvidraco Species 0.000 title claims abstract description 71
- 238000009396 hybridization Methods 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 24
- 241000251468 Actinopterygii Species 0.000 claims abstract description 33
- 230000035479 physiological effects, processes and functions Effects 0.000 claims abstract description 27
- 230000008771 sex reversal Effects 0.000 claims abstract description 18
- 238000009395 breeding Methods 0.000 claims abstract description 16
- 230000001488 breeding effect Effects 0.000 claims abstract description 15
- 241000746812 Tachysurus vachellii Species 0.000 claims abstract description 13
- 230000012010 growth Effects 0.000 claims abstract description 9
- 230000013011 mating Effects 0.000 claims abstract description 9
- 230000009027 insemination Effects 0.000 claims description 16
- 229940011871 estrogen Drugs 0.000 claims description 13
- 239000000262 estrogen Substances 0.000 claims description 13
- 238000011031 large-scale manufacturing process Methods 0.000 claims description 7
- 235000019733 Fish meal Nutrition 0.000 claims description 5
- 239000004467 fishmeal Substances 0.000 claims description 5
- 241001247197 Cephalocarida Species 0.000 claims description 3
- 238000012795 verification Methods 0.000 claims description 3
- 210000001061 forehead Anatomy 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 5
- 238000010924 continuous production Methods 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 230000002068 genetic effect Effects 0.000 abstract description 2
- 230000006641 stabilisation Effects 0.000 abstract description 2
- 238000011105 stabilization Methods 0.000 abstract description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 241001471302 Tachysurus Species 0.000 description 3
- 229960000583 acetic acid Drugs 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000009402 cross-breeding Methods 0.000 description 2
- 238000002224 dissection Methods 0.000 description 2
- 239000012362 glacial acetic acid Substances 0.000 description 2
- 230000002710 gonadal effect Effects 0.000 description 2
- 230000000366 juvenile effect Effects 0.000 description 2
- 210000001672 ovary Anatomy 0.000 description 2
- 241000252496 Siluriformes Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013332 fish product Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of methods that quick foundation hybridizes XY ♂ all-male Pelteobagrus fulvidracos, it is to be mated with common Pelteobagrus fulvidraco raun (XX ♀) by genetic method cultivation acquisition hybridization YY ♂ supermales fishes based on Pelteobagrus fulvidraco YY ♀ physiology rauns and the mating filial generation of Pelteobagrus vachelli XY ♂ milters and produce hybridization XY ♂ all-male Pelteobagrus fulvidracos.The method of the present invention can be obviously shortened breeding cycle, compared with the technologies such as Varadaraj, can shorten for 2 generations, can shorten for 1 generation compared with the method for gynogenesis continuous production all-male Pelteobagrus fulvidraco with sex reversal is used.In addition, the hybridization all-male Pelteobagrus fulvidraco stabilization characteristics of genetics that the method for the present invention obtains, the speed of growth is fast, and individual is big, and strong environmental adaptability not only has heterosis, hybrid vigor, and inherits the advantage of all-male fish, can obtain preferably cultivation and economic benefit.
Description
Technical field
The present invention relates to the breedings of hybridization Pelteobagrus fulvidraco, and in particular to a kind of side of quick foundation hybridization XY ♂ all-male Pelteobagrus fulvidracos
Method.
Background technology
Pelteobagrus fulvidraco and Pelteobagrus vachelli belong to siluriformes, Chang sections, and Pelteobagrus is that economic value is higher in Pelteobagrus
Two types.Both fishes are because of its fine and tender taste, delicious flavour, full of nutrition, without being pierced between flesh, deep to be liked by consumers in general,
It is the important fresh water Quality and economy fish in China.Market demand is in increased situation year by year, and supply falls short of demand in market.China is certainly
The artificial propagation of two kinds of fish is proceeded by the beginning of 21 century and is raised and train, and successful.In recent years, the big rule of both fishes
Mould artificial breeding is just risen throughout the country, and the artificial breeding of especially Pelteobagrus fulvidraco is more extensive.
However, there is larger differences on biological and ecological characteristics for both fishes.Pelteobagrus fulvidraco is mainly distributed on length
In the lake of river middle and lower reaches, under identical cultivating condition, an age male Pelteobagrus fulvidraco produces fast by 30%~50%, two ages life than raun
Produce fast 1~2 times of speed.Its individual is smaller, and the speed of growth is slower, and in highdensity pond culture, current year seed is more difficult to be grown to
Listing specification (50g or more), but to strong environmental adaptability.2 rheological properties of Pelteobagrus vachelli is ripe, in rivers water body and artificial feeding
Fish male and female growth individual difference is also larger in pond, and milter is more than raun usually 40%~52.5%, and some milters are more than
By about one time above (Cai Yan values etc., 2003), current year seed can grow to listing specification to raun, be produced in Pelteobagrus it is most fast,
The maximum type of individual, but it is poor to adaptive capacity to environment.
Currently, inventor has invented using sex reversal and gynogenesis continuous production all-male Pelteobagrus fulvidraco (referring to Chinese special
Sharp publication number CN1994072A, publication date on July 11st, 2007, patent name：Sex reversal and gynogenesis continuous production all-male
Pelteobagrus fulvidraco method), breeding time is shortened, economic benefit is improved.However compared to Pelteobagrus vachelli, all-male Pelteobagrus fulvidraco individual
Still smaller, inhereditary feature is unstable and the speed of growth is slow.
Invention content
In order to further increase the quality and breeding speed of Yellow cartfish adult, acquisition individual is larger, the speed of growth is very fast,
Current year seed grows to commercial specification, adaptable hybridization all-male Pelteobagrus fulvidraco new varieties.Cultivation of the present invention all-male Pelteobagrus fulvidraco
Method combines with Pelteobagrus fulvidraco with the method for Pelteobagrus vachelli crossbreeding, provides a kind of quickly establish and hybridizes XY ♂ all-males
The method of Pelteobagrus fulvidraco.
The method of the quick foundation hybridization XY ♂ all-male Pelteobagrus fulvidracos, includes the following steps：
(1) hybridize the artificial propagation of Pelteobagrus fulvidraco：Select body surface without wound, individual is big, sexually matured Pelteobagrus fulvidraco YY ♀ physiology is female
Fish and Pelteobagrus vachelli XY ♂ milters, artificial insemination, all milters of fry of acquisition；Part fry routinely feeds to sexal maturity,
Another part fry carries out artificial sex reversal with estrogen and feeds to sexal maturity；
(2) hybridize YY ♀ physiology raun parent's selection and breeding：The sexal maturity fish of sex reversal, it is yellow with XY ♂ Pelteobagrus fulvidracos or XY ♂ Wa Shi
All filial generation fries of forehead fish test cross, every raun test cross were raised respectively to after three months, the filial generation fry of each mating combination
A part is separated for dissecting identification gender, the tester of all milters of filial generation is screened, as hybridizes YY ♀ physiology rauns
Parent；
(3) hybridize the acquisition of YY ♂ supermale Pelteobagrus fulvidracos：After the fry growth to sexal maturity of conventinal breeding, essence is manually taken, every
A sperm part for fish hybridizes YY ♀ physiology raun parents progress artificial insemination, another part and XX ♀ with raising to sexually matured
Pelteobagrus fulvidraco or XX ♀ Pelteobagrus vachelli artificial insemination are verified for test cross；After artificial insemination, the filial generation fry of each mating combination
It raises respectively；Fry for test cross verification was fed to after three months, takes out a part for dissecting identification gender, screens filial generation
The tester of all milters, as YY ♂ supermales Pelteobagrus fulvidraco parent retain the parent and are awarded with YY ♀ physiology raun parents are hybridized
The offspring of essence is to hybridize YY ♂ supermale Pelteobagrus fulvidracos；
(4) hybridization YY ♀ physiology raun parents and the quick group expanding for hybridizing YY ♂ supermale yellow cartfish breeding populations：Take hybridization YY
♀ physiology raun parents carry out artificial insemination with YY ♂ supermale Pelteobagrus fulvidracos are hybridized, and generate 100% hybridization YY ♂ supermale fishes, take part fish
Seedling carries out artificial sex reversal with estrogen becomes hybridization YY ♀ physiology rauns；
(5) large-scale production hybridizes XY ♂ all-male Pelteobagrus fulvidracos：Hybridization YY ♂ supermales fishes mate with common Pelteobagrus fulvidraco XX ♀ rauns,
Be produced on a large scale hybridization XY ♂ all-male Pelteobagrus fulvidracos.
Wherein, 100% hybridization YY ♂ supermale fishes in step (4), taking part to carry out artificial sex reversal with estrogen becomes miscellaneous
Hand over YY ♀ physiology rauns, it is therefore an objective to which establishing stable YY crossbreeds system, (namely genotype is the hybridization supermale Pelteobagrus fulvidraco of YY
Breeding population), so that large-scale production hybridizes XY ♂ all-male Pelteobagrus fulvidracos, and YY crossbreeds system is needed by hybridizing YY ♀ physiology
What raun and hybridization YY ♂ supermale fishes mating obtained.
" mating combination " described in the method for the present invention refers to the group of the male parent fish and a maternal fish that mate
It closes." routinely the feeding " refers to being used only that the fish meal of fish vegetative growth is helped to feed, and can be led without adding other for example
The fish meal for causing piscinity not change.
Crossbreeding is a kind of classical, traditional breeding method, and in China, the utilization of heterosis, hybrid vigor, which has become, improves fish production
Amount and the important channel for improving fish products matter.By hybridization, recombination of genes can be made, acquisition may be in the speed of growth, degeneration-resistant
Property and meat etc. show the offspring of hybrid vigour.
The quick method for establishing hybridization XY ♂ all-male Pelteobagrus fulvidracos of the present invention, can be quickly obtained with Pelteobagrus fulvidraco and Wa Shi
The hybridization all-male Pelteobagrus fulvidraco new varieties of Pelteobagrus fulvidraco parents merit or super parents' merit, to be production practices service.
Preferably, in the method for above-described quick foundation hybridization XY ♂ all-male Pelteobagrus fulvidracos, the property described in step (1) is inverse
Turn to be the carrier using artemia larvae as estrogen after fry initial feeding, feeds fry, after continuously feeding 40 days, change
It is fed to sexal maturity with the fish meal without estrogen.
Compared with prior art, the invention has the advantages that：
Hybridization supermale Pelteobagrus fulvidraco (hybridizing YY ♂ supermales fish) and the common Pelteobagrus fulvidraco that the present invention is cultivated by genetic method
Raun (XX ♀) mating production hybridization XY ♂ all-male Pelteobagrus fulvidracos, shorten breeding cycle, can be with compared with the technologies such as Varadaraj
Shortened for 2 generations, with published " the sex reversal and gynogenesis continuous production all-male Pelteobagrus fulvidraco method " patent (publication number of inventor
CN1994072A it) compares, can shorten for 1 generation.Three kinds of technology paths compare as shown in appendix 1.In addition, the method for the present invention obtains
Hybridization all-male Pelteobagrus fulvidraco stabilization characteristics of genetics, the speed of growth is fast, and individual is big, strong environmental adaptability, and it is excellent not only to have hybridization
Gesture, and the advantage of all-male fish is inherited, preferably cultivation and economic benefit can be obtained.
Description of the drawings
Fig. 1 is that the present invention establishes the total Technology Roadmap for hybridizing XY ♂ all-male Pelteobagrus fulvidracos；
Fig. 2 is that hybridization YY ♀ physiology raun parents verify schematic diagram with the test cross for hybridizing YY ♂ supermale Pelteobagrus fulvidracos in the present invention.
Specific implementation mode
The present invention is further explained in the light of specific embodiments, so that those skilled in the art can be better
Understand the present invention and can be practiced, but illustrated embodiment is not as a limitation of the invention.
The method for building up of the hybridization XY ♂ all-male Pelteobagrus fulvidracos of the present embodiment specifically includes following three steps：
1, the acquisition of YY ♀ physiology raun parent
Select body surface without wound, big, the sexually matured Pelteobagrus fulvidraco YY ♀ physiology rauns of individual and Pelteobagrus vachelli XY ♂ milters with now
There is conventional method to carry out artificial induced spawning and insemination, all milters of F1 generation (the 50% miscellaneous miscellaneous YY ♂ of XY ♂ and 50%) of acquisition.Take portion
Divide first familiar generation fry, after fry initial feeding, carries out artificial sex reversal with estrogen, method is to use artemia larvae conduct
The carrier of estrogen, feeds fry, after continuously feeding 40 days, uses the fish meal without estrogen instead and feeds to sexal maturity.
Miscellaneous XY ♂ sex reversals after Estrogenization become miscellaneous XY ♀, miscellaneous YY ♂ sex reversals become miscellaneous YY ♀.By sex reversal
Miscellaneous YY ♀ and the raun of miscellaneous XY ♀ support to sexal maturity, and with common XY ♂ Pelteobagrus fulvidracos or XY ♂ Pelteobagrus vachelli test crosses.By existing
There is conventional method to carry out artificial induced spawning and insemination, after artificial insemination, the offspring of every fish test cross temporarily supports in small-sized net cage respectively.Deng
Fry routine feeding from each test cross combination (i.e. mating combination) to after three months, taking out one third juvenile fish by tail abdominal cavity note
Glacial acetic acid fixer is penetrated, differentiates spermary or ovary after dissection from Gonadal Morphology, analyzes sex ratio.If test cross offspring Quan Shixiong
Property fish, then its parent be hybridization YY ♀ physiology raun parents, it is spare.
2, hybridization YY ♂ supermale Pelteobagrus fulvidracos are obtained
After filtering out hybridization YY ♀ physiology raun parents, and the first familiar generation milter of another part (without sex reversal, miscellaneous XY
♂ and miscellaneous YY ♂) test cross verification is carried out, obtain candidate miscellaneous YY ♂ (i.e. YY ♂ supermales Pelteobagrus fulvidraco parent).Because when artificial insemination, it is necessary to
Milter is killed, cuts open the belly and takes essence, so same tail milter must mate with XX ♀ Pelteobagrus fulvidracos or XX ♀ Pelteobagrus vachellis, and and YY
♀ physiology rauns mate.Per tail milter post-coitum, in generation, temporarily supports in small-sized net cage respectively, and fries is waited to feed to after three months, from each
One third juvenile fish is taken out in test cross combination, glacial acetic acid fixer is injected intraperitoneally by tail, differentiate spermary after dissection from Gonadal Morphology
Or ovary, analyze sex ratio.If test cross offspring is male fish entirely, thus it is speculated that its parent is miscellaneous YY ♂ (i.e. YY ♂ supermales Pelteobagrus fulvidraco parents
This), while retaining the offspring that this miscellaneous YY ♂ milter mates with hybridization YY ♀ physiology raun parents：Hybridize YY ♂ supermale Pelteobagrus fulvidracos.
3, hybridization YY ♂ supermale yellow cartfish breedings system is established
It takes hybridization YY ♀ physiology raun parents to carry out artificial insemination with YY ♂ supermale Pelteobagrus fulvidracos are hybridized, generates 100% hybridization YY
♂ supermale fishes, taking part fry to carry out artificial sex reversal with estrogen becomes hybridization YY ♀ physiology rauns, to quickly establish hybridization
Supermale yellow cartfish breeding population (YY crossbreeds system), and the hybridization of the production of hybrid seeds is provided for large-scale production XY ♂ all-male Pelteobagrus fulvidracos
YY ♂ supermale fishes.
4, large-scale production hybridizes XY ♂ all-male Pelteobagrus fulvidracos
Hybridization YY ♂ supermales fishes mate with common Pelteobagrus fulvidraco XX ♀ rauns, you can large-scale production is miscellaneous.
1 three kinds of technical solution technical process of subordinate list compare
Embodiment described above is only to absolutely prove preferred embodiment that is of the invention and being lifted, protection model of the invention
It encloses without being limited thereto.Those skilled in the art on the basis of the present invention made by equivalent substitute or transformation, in the present invention
Protection domain within.Protection scope of the present invention is subject to claims.
Claims (2)
1. a kind of method of quick foundation hybridization XY ♂ all-male Pelteobagrus fulvidracos, which is characterized in that include the following steps：
(1) hybridize the artificial propagation of Pelteobagrus fulvidraco：Select body surface without wound, big, the sexually matured Pelteobagrus fulvidraco YY ♀ physiology rauns of individual and
Pelteobagrus vachelli XY ♂ milters, artificial insemination, all milters of fry of acquisition；Part fry routinely feeds to sexal maturity, another
Part fry carries out artificial sex reversal with estrogen and feeds to sexal maturity；
(2) hybridize YY ♀ physiology raun parent's selection and breeding：The sexal maturity fish of sex reversal, with XY ♂ Pelteobagrus fulvidracos or XY ♂ Pelteobagrus vachellis
All filial generation fries of test cross, every raun test cross were raised respectively to after three months, and the filial generation fry of each mating combination separates
A part screens the tester of all milters of filial generation, as hybridizes YY ♀ physiology raun parents for dissecting identification gender；
(3) hybridize the acquisition of YY ♂ supermale Pelteobagrus fulvidracos：After the fry growth to sexal maturity of conventinal breeding, essence is manually taken, every fish
A sperm part hybridizes YY ♀ physiology raun parents progress artificial insemination, another part and XX ♀ Huang foreheads with raising to sexually matured
Fish or XX ♀ Pelteobagrus vachelli artificial insemination are verified for test cross；After artificial insemination, the filial generation fry of each mating combination is distinguished
Raising；Fry for test cross verification was fed to after three months, took out a part for dissecting identification gender, screening filial generation is whole
For the tester of milter, as YY ♂ supermales Pelteobagrus fulvidraco parent, retains the parent and hybridize YY ♀ physiology raun parents insemination
Offspring is to hybridize YY ♂ supermale Pelteobagrus fulvidracos；
(4) hybridization YY ♀ physiology raun parents and the quick group expanding for hybridizing YY ♂ supermale yellow cartfish breeding populations：Take hybridization YY ♀ lifes
Reason raun parent carries out artificial insemination with YY ♂ supermale Pelteobagrus fulvidracos are hybridized, and generates 100% hybridization YY ♂ supermale fishes, part fry is taken to use
Estrogen, which carries out artificial sex reversal, becomes hybridization YY ♀ physiology rauns；
(5) large-scale production hybridizes XY ♂ all-male Pelteobagrus fulvidracos：Hybridization YY ♂ supermales fishes mate with common Pelteobagrus fulvidraco XX ♀ rauns, you can
Large-scale production hybridizes XY ♂ all-male Pelteobagrus fulvidracos.
2. the method for quick foundation hybridization XY ♂ all-male Pelteobagrus fulvidracos according to claim 1, which is characterized in that step (1) institute
The sex reversal stated is after fry initial feeding, and the carrier using artemia larvae as estrogen feeds fry, continuously feeds 40
After it, uses the fish meal without estrogen instead and feed to sexal maturity.
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| CN105918184B (en) * | 2016-06-14 | 2018-08-21 | 武汉百瑞生物技术有限公司 | A kind of method of selection cross all-male Pelteobagrus fulvidraco improved seeds |
| CN106489799B (en) * | 2016-11-16 | 2019-05-17 | 唐志发 | The method that breeding is sheerly the full raun of Pelteobagrus fulvidraco and supermale fish large-scale production all-male fish |
| CN106577388B (en) * | 2016-12-14 | 2019-08-06 | 华中农业大学 | A kind of cross breeding method of excellent No. 1 Pelteobagrus fulvidraco of Huang of improvement growth traits |
| CN107094671A (en) * | 2017-05-19 | 2017-08-29 | 佛山市南海百容水产良种有限公司 | A kind of all-male hybridizes the breeding method of Pelteobagrus fulvidraco |
| CN109169415A (en) * | 2018-07-24 | 2019-01-11 | 武汉百瑞生物技术有限公司 | A kind of production method of all-male snakehead |
| CN110583539B (en) * | 2019-10-25 | 2021-05-28 | 西南大学 | Method for efficiently producing Nile tilapia high-reproduction-rate YY-converted female fish |
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| CN101390503A (en) * | 2008-11-03 | 2009-03-25 | 水利部中国科学院水工程生态研究所 | Method for establishing super-male yellow cartfish breeding population by copulating super-male fish with physiology spawner |
| CN102960280A (en) * | 2012-11-30 | 2013-03-13 | 射阳康余水产技术有限公司 | Method for cultivating super-male fish and fully-male fish of yellow catfish by genetic method |
| CN103798169A (en) * | 2014-02-20 | 2014-05-21 | 武汉百瑞生物技术有限公司 | Method for quickly establishing improved breeding system of YY super-male pelteobagrus fulvidraco |
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2015
- 2015-11-23 CN CN201510818624.9A patent/CN105360031B/en not_active Expired - Fee Related
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1340298A (en) * | 2000-08-25 | 2002-03-20 | 武汉康祥科技发展有限公司 | Technique for creating super-male fish reproduction system |
| CN1994072A (en) * | 2006-12-28 | 2007-07-11 | 中国科学院水生生物研究所 | Method for continuously producing complete male pelteobagrus fulvidraco by sex reversion and embryonic development |
| CN101390503A (en) * | 2008-11-03 | 2009-03-25 | 水利部中国科学院水工程生态研究所 | Method for establishing super-male yellow cartfish breeding population by copulating super-male fish with physiology spawner |
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