CN102124997B - Method for breeding two-point-pattern lacy duck new breed - Google Patents
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Abstract
The invention relates to a method for breeding a two-point-pattern lacy duck new breed. The method comprises the following steps of: 1) selecting Sichuan lacy ducks as materials and inbreeding two generations, wherein the Sichuan lacy ducks are hybridized by white feather Tianfu meat male ducks and Sichuan local female sheldrakes; 2) hybridizing male ducks obtained in the step 1) with female cherry valley ducks to generate hybrid lacy ducks, performing appearance selection for three generations, and building the hybrid lacy duck F1 generation by the remained individuals; 3) performing early breeding on the hybrid lacy duck F1 generation by using a molecular marker of an MC1R gene; 4) performing family breeding on the obtained remained individuals and performing closed breeding to generate F2 generation; and 5) hybridizing the F2 generation individuals with Aobaixing Duck or Beijing ducks to generate the lacy ducks with a small amount of black feather on the heads and the tails. The method has the advantages that: hybrid combination is performed on the basis of natural group lacy ducks; and the meat duck new breed with consistent appearance and stable heritability are bred by the traditional breeding method and the modern molecular breeding method.
Description
Technical field
The present invention relates to the poultry breeding method, relate in particular to a kind of lace duck new lines producing method for seed of 2 flowers.
Background technology
The lace duck has another name called 4 colored ducks, and being the Growth of Cherry Valley kind forms with the sheldrake inter breed crossing, and Bai Yu self-sufficient and strategically located region meat duck drake hybridizes with Sichuan sheldrake duck and forms, and also someone thinks a kind of in the sheldrake of Guangxi to belong to meat egg dual-purpose type duck kind.When going out shell, take yellow feather as main, at head, neck, chest and back or afterbody have white, brown, black assorted feather mutually.Grow up moult after, the part hair color is entirely white, above brown or black plumage is arranged everywhere, the spitting image of the lace in traditional clothes, so gain the name.Present lace drake feather look disunity, the black plumage band distributes more chaotic, disorderly and unsystematic, consumer and raiser when choosing, the duck of being confused about that a kind of plumage look is the lace duck, often have the swindle phenomenon to produce in process of exchange, and present lace duck does not reach a fine very pure external appearance characteristic in consumer's eye.
Sichuan is major production base and the consumption market of China meat duck, and variety type is mainly large-scale meat duck and lace duck.The former fast growth, the price of deed is high, but is subject to larger limitation take roasting as the consumption of main eating method in the zone, southwest.The lace duck is traditional eating method of locality, very suitable Sichuan, color, smell and taste are complete, but this kind is not all passed through systematic breeding, the outward appearance disunity, buy constant error solution often the consumer, and growth rate is slow, productivity is low, feeding cost is higher, and raiser and consumer have been produced certain restriction.At present, the shared meat duck in lace duck consumption market ratio is more than 60%.In order further to promote the development that the duck industry is supported in Sichuan, accomplish not rely on from raising the family scattered and advance to plant, reduce production costs simultaneously, the actual conditions in calmodulin binding domain CaM market and Sichuan, carry out " seed selection of lace duck and Application and Development ", cultivate and have better productivity the lace duck new lines of (growth is very fast, the price of deed high), the demand of satisfying the market is enhanced competitiveness.
Summary of the invention
the lace duck new lines producing method for seed that the purpose of this invention is to provide a kind of 2 flowers, carry out the corss combination research work take the fast large-scale duck kind (French white star duck difficult to understand) of the lace duck specialized strain of existing high-quality Sichuan province and local breeding and introduction as main material, each side's advantage is organically combined, foundation has certain growth speed, the fast large-scale lace duck breeding of method that meat is good, carry out intensive production, simultaneously in conjunction with modern molecule breeding technique-by melanocortin receptor 1 (Melanocortin 1 receptor, MC1R) (the MC1R gene is the important gene of controlling the boneblack pigment synthesis to gene, at melanocyte, adrenal cortical cell, show different functions in nervous system and immune system) the mutational site carry out seed selection, seed selection in early days in practice, purifying lace drake feather look, produce lace duck-" 2 flowers end to end " of obvious characteristic, unified and obvious feature is arranged, make raiser and consumer to be easy to and recognize very accurately this strain, to overcome the prior art above shortcomings.
The objective of the invention is to be achieved through the following technical solutions:
A kind of lace duck new lines producing method for seed of 2 flowers comprises the following steps:
1) select the Sichuan lace duck that forms take Bai Yu self-sufficient and strategically located region meat duck drake and Sichuan sheldrake duck selection cross as material, from lace duck colony selective body great (greater than 2kg), the bodily form is wide, neck is thick, 100 of fully-fledged individual drakes are set up into paternal underlying group with 700 of ducks; From opening the lace duck colony of product, next group select the bodily form 130 of individual drakes good, that full-fledged, black wool is few to set up into maternal underlying group with 900 of ducks, two generations of the inbreeding production of hybrid seeds;
2) through step 1) in the Sichuan lace duck seed selection group that obtains, selective body is heavy, the bodily form is wide, neck is thick, fully-fledged drake, again Sichuan lace duck seed selection group's drake and the duck hybridization of cherry valley duck are produced hybridization lace duck, hybridization lace duck carries out outward appearance again and selects the three generations, reject numb plumage and Bai Yu individual, produce hybridization lace duck F1 generation;
3) molecular labeling that utilizes the MC1R gene is to through step 2) hybridization lace duck F1 generation of obtaining chooses seeds in early days, and according to molecular labeling seed selection result and hatching constantly the phenotype result select the individuality of reserving seed for planting; Its early stage seed selection comprises the following steps:
(1) hybridization lace duck F1 was for rear 7 days of birth, and venous blood 1mL is adopted at the place at Duck wing; Draw blood 20uL utilizes " phenol/chloroform recovery method " to extract DNA;
(2) utilize MC1R gene magnification primer to carry out pcr amplification, template is the DNA sample in hybridization lace duck F1 generation of extracting by step (1), and the amplification total length is 754bp; Check order, obtain the nucleotide sequence that increases;
When carrying out pcr amplification, the PCR reaction system is 30uL, comprises the full genomic templates DNA of 3uL, 2 * Taq Master Mix of 15uL, the upstream primer of 1uL and the downstream primer of 1uL, the ddH2O of 10uL; Amplified reaction is all completed on the PCR instrument;
The PCR reaction condition is as follows:
(a) 95 ℃ of denaturation 4min;
(b) 94 ℃ of sex change 30s;
(c) annealing temperature 53.5 degree 45s;
(d) 72 ℃ are extended 50s;
(e) step (b)-(d) repeats 35 times;
(f) 72 ℃ are extended 15min.
(3) according to the nucleotide sequence that increases, select to satisfy simultaneously in MC1R gene 411 CC of place genotype and the 756 genotypic individualities of the GG of place to reserve seed for planting, all the other individual eliminating.
4) will be by step 3) individuality of reserving seed for planting that obtains sets up the family group in breeding objective with the male and female ratio of 1: 3 respectively and carries out the family breeding, seals to breed to produce F2 generation;
5) will be by step 4) F2 that obtains is for individual and white star duck difficult to understand or Beijing duck hybridization, and produce head and afterbody and all have a small amount of black plumage, and health remainder major part is white lace duck.
In the lace duck new lines producing method for seed of 2 flowers described above, described MC1R gene magnification primer is:
Upstream primer is 5 '-GCCCAATGAACTCTTCCTCACG-3 ', and downstream primer is 5 '-GCACCGAGTTGCAGATGATGAG-3 '.
Beneficial effect of the present invention is:
1) save time and money, if conventional outward appearance seed selection needs 8 years at least, what this method seed selection was total has only spent 5 years; The binding molecule seed selection need not wait duck to grow up, and only need to adopt a blood in rear 7 days in birth, extracts the DNA-PCR-order-checking, comparison, these experiments just can be completed in 3 days, satisfactory reserving seed for planting, undesirable eliminating, the duck of eliminating only need not be raised again, directly can sell the saving cost; Secondly, early stage seed selection (be born and just began afterwards in 7 days) has reduced breeding expenses, feed cost, place rent with expense, immune defense expense and labour cost etc.; Due to the seed selection time that shortens, be shorten-originally to wait duck to grow up the generation interval, observe plumage look separation case about 50 days, now if about 10 days just can, and can eliminate undesirablely, ceaselessly hatch simultaneously, ceaselessly seed selection, cultivation that need not be conventional-every a collection of will arriving about 50 days selected again, need not consider every a collection of duck feeding cost only, space, place that takies etc.
2) accuracy, if only utilize the molecule seed selection, because feather color probably is subject to the control of a plurality of genes simultaneously, and being difficult to now determine is those several genes, the present invention combines conventional outward appearance seed selection, carry out again the molecule seed selection on the basis of outward appearance seed selection, obtain that appearance is consistent, heritability is stable, maternal lay heavily, paternal line are produced meat duck new lines faster in early days.
Description of drawings
Fig. 1 is " 2 flowers end to end " lace duck production of hybrid seeds flow chart of determining in the lace duck new lines producing method for seed of described a kind of 2 flowers of the embodiment of the present invention.
Embodiment
The lace duck new lines producing method for seed of described a kind of 2 flowers of the embodiment of the present invention comprises the following steps:
1) select the Sichuan lace duck that forms take Bai Yu self-sufficient and strategically located region meat duck drake and Sichuan sheldrake duck selection cross as material, from lace duck colony selective body great (greater than 2kg), the bodily form is wide, neck is thick, 100 of fully-fledged individual drakes are set up into paternal underlying group with 700 of ducks; From opening the lace duck colony of product, next group select the bodily form 130 of individual drakes good, that full-fledged, black wool is few to set up into maternal underlying group with 900 of ducks, two generations of the inbreeding production of hybrid seeds;
2) through step 1) in the Sichuan lace duck seed selection group that obtains, selective body is great, the bodily form is wide, neck is thick, fully-fledged drake, again Sichuan lace duck seed selection group's drake and the duck hybridization of cherry valley duck are produced hybridization lace duck, hybridization lace duck carries out outward appearance again and selects the three generations, rejects numb plumage and Bai Yu individuality; Produce hybridization lace duck F1 generation;
3) molecular labeling that utilizes the MC1R gene is to through step 2) hybridization lace duck F1 generation of obtaining chooses seeds in early days, and according to molecular labeling seed selection result and hatching constantly the phenotype result select the individuality of reserving seed for planting; Its early stage seed selection comprises the following steps:
(1) hybridization lace duck F1 was for rear 7 days of birth, and venous blood 1mL is adopted at the place at Duck wing; Draw blood 20uL utilizes " phenol/chloroform recovery method " to extract DNA;
(2) utilize MC1R gene magnification primer to carry out pcr amplification, template is the DNA sample in hybridization lace duck F1 generation of extracting by step (1), and the amplification total length is 754bp; Check order, obtain the nucleotide sequence that increases;
When carrying out pcr amplification, the PCR reaction system is 30uL, comprises the full genomic templates DNA of 3uL, 2 * Taq Master Mix of 15uL, the upstream primer of 1uL and the downstream primer of 1uL, the ddH2O of 10uL; Amplified reaction is all completed on the PCR instrument;
The PCR reaction condition is as follows:
(a) 95 ℃ of denaturation 4min;
(b) 94 ℃ of sex change 30s;
(c) annealing temperature 53.5 degree 45s;
(d) 72 ℃ are extended 50s;
(e) step (b)-(d) repeats 35 times;
(f) 72 ℃ are extended 15min.
(3) according to the nucleotide sequence that increases, select to satisfy simultaneously in MC1R gene 411 CC of place genotype and the 756 genotypic individualities of the GG of place to reserve seed for planting, all the other individual eliminating.
4) will be by step 3) individuality of reserving seed for planting that obtains sets up the family group in breeding objective with the male and female ratio of 1: 3 respectively and carries out the family breeding, seals to breed to produce F2 generation;
5) will be by step 4) F2 that obtains is for individual and white star duck difficult to understand or Beijing duck hybridization, and produce head and afterbody and all have a small amount of black plumage, and health remainder major part is white lace duck.
In the lace duck new lines producing method for seed of 2 flowers described above, described MC1R gene magnification primer is:
Upstream primer is 5 '-GCCCAATGAACTCTTCCTCACG-3 ', and downstream primer is 5 '-GCACCGAGTTGCAGATGATGAG-3 '
Test
(1) experiment material
White star duck difficult to understand is to be adopted the commercial meat duck of strain matching method seed selection by France white star difficult to understand company.Have that build is large, growth is fast, precocious, easily fertilizer and dressing percentage advantages of higher.This duck property happiness is dry, can carry out on the ground natural mating, adapts to nonirrigated farmland stable breeding or online the raising.What this experiment was used is white star 2000 type meat ducks difficult to understand, pure white.
Beijing duck, Beijing duck are world-renowned good meat duck standard varieties.Most of pure white, have grow fast, fatten the good characteristics of performance, be the making raw material of " Beijing roast duck " well-known at home and abroad.Originate in the Yuquanshan Mountain, west suburb in Beijing one band, now spread all over all over the world, support in the duck industry in the world and occupy critical role.
Cherry valley duck, cherry valley duck once came from Chinese duck kind, optimize through the Englishman and " hit back to " Chinese market from Britain again after breeding, and were world-renowned lean meat species ducks.Have that growth is fast, lean meat percentage is high, pure meat percentage is high and feed conversion rate is high, and the advantage such as premunition is strong, build is larger, adult weight drake 4.0-4.5 kilogram, duck 3.5-4.0 kilogram.In 26 ages in week of father and mother Dai Qun duck sexual maturing period, annual is laid eggs 210-220 piece.White plumage L is 3.0 kilograms of commodity duck 47 age in days body weight, material anharmonic ratio 3: 1, and lean meat percentage reaches more than 70%, brisket rate 23.6%-24.7%.
Sichuan lace duck breeding group (being called for short H) builds for this experiment is special.Construction method is as follows:
The Sichuan lace duck that selection forms take Bai Yu self-sufficient and strategically located region meat duck drake and Sichuan sheldrake duck selection cross is as material, and selective body great (greater than 2kg), the bodily form wide (chest breadth is greater than 12.5 centimetres), neck thick (duck neck width is greater than 3 centimetres), 100 of fully-fledged individual drakes are set up into paternal underlying group with 700 of ducks from lace duck colony; From opening the lace duck colony of product, next group select the bodily form 130 of individual drakes good, that full-fledged, black wool is few to set up into maternal underlying group with 900 of ducks, two generations of the inbreeding production of hybrid seeds.
(2) experimental cross forms a complete set of:
Remarks: be paternal in bracket, the drake of only selecting and remain.
4 experimental group paternal line { (Growth of Cherry Valley ♀ X Sichuan lace duck ♂) ♂, (Growth of Cherry Valley ♀ X Sichuan lace duck ♂) }, perhaps maternal { (Growth of Cherry Valley ♀ X Sichuan lace duck ♂) ♀, (Growth of Cherry Valley ♀ X Sichuan lace duck ♂) ♀ } all pass through outward appearance three generations's seed selection, reduce numb plumage in a large number at first in appearance.secondly the paternal or maternal F1 that produces respectively is for individuality, adopt the Duck wing venous blood 1mL of place when 7 age in days, draw blood 20uL, utilize " phenol/chloroform recovery method " to extract DNA, utilize MC1R gene magnification primer to carry out pcr amplification to the MC1R gene, after send the order-checking of the large genome company of China with amplification, then compare sequencing result, selection result satisfies simultaneously in 411 CC of place genotype and the 756 genotypic individualities of the GG of place reserves seed for planting, all the other individual eliminating, the individuality of reserving seed for planting is set up family in breeding objective with the male and female ratio of 1: 3 respectively and is carried out the family breeding, seal to breed and produce F2 generation.F2 is hybridized with white star duck difficult to understand or Beijing duck for individuality again.
(3) index determining and method
The hatching constantly hatching of phenotype is determined as: select bouncing, hair color meets the individuality that kind requires, paternal the first two adopts the method for mass selection from generation to generation, and when setting up family colony from the third generation, hatching just requires to wear wing number; Differentiate male and female during maternal hatching and begin to wear wing and number reserve seed for planting, and carry out detailed pedigree and individual registration.During raising, male and female is separately raised, and each requires to be no less than 2000 seedlings the hatching male and female.
Seed selection and feed conversion rate are measured: 6 age in week the foot couple drake carry out whose body weight and select, select simultaneously hair color and other correlated traits, 600 drakes of selecting and remain carry out feed conversion rate and measure; Feed conversion rate is measured 150-180 the drake of selecting and remain after a week and is reserved seed for planting; Hair color and other correlated traits are selected in the direct body weight selection in 7 ages in week of duck simultaneously.
Slaughter determining: the Slaughter of suitably being correlated with when 6,7 age in week is measured.
The mensuration of reproductive performance and selection: carry out mainly for maternal genealogy, carry out individual egg-laying test, recording individual is opened the product time, opens and lays eggs heavily, lay eggs situation and set up family and raise up seed of computer statistics family during record statistics 300 age in days.The selection of fertilization rate, incubation rate is planted drake and is selected in conjunction with the routine inspection of semen quality take pedigree record and offspring's achievement as selecting foundation.
(4) experimental result:
Can be found out by table 1-table 8:
Table 1, hybridization lace duck different generations duckling plumage look separation case
As can be seen from Table 1, hybridization lace duck appearance reaches unanimity: adopt the homogeneity apolegamy of phenotype during successive propagation, the plumage look of colony more reaches unanimity, and the 3rd from generation to generation, and numb plumage, white plumage ratio obviously reduce.
Table 2, experimental group lethality
Experimental group at brood (0-7 week age), breed (8-21 age in week), the lethality of laying eggs the stage (21-64 age in week) relatively in, experimental group YHA combination lethality is all lower, vitality is improved significantly.
Table 3, experimental group reproductive performance average unit: day, piece
Experimental group reproductive performance average relatively, the YHA group is opened and is produced age in days early, egg production is high, laying period lethality low, YHB group fertilization rate is high, generally speaking YHA group reproductive performance is best.
Table 4, experimental group kind Egg Quality are measured situation
| Test combinations | Quantity (n) | Egg size (g) | Major axis (mm) | Minor axis (mm) | The egg shape index |
| YHB | 189 | 81.52±5.68 | 66.23±2.28 | 46.73±1.43 | 1.42±0.06 |
| BYH | 236 | 81.49±5.56 | 65.52±2.62 | 46.33±1.74 | 1.42±0.07 |
| YHA | 230 | 81.42±5.41 | 65.92±2.22 | 46.80±1.23 | 1.41±0.05 |
| AYH | 235 | 81.96±5.97 | 68.22±2.88 | 47.93±1.93 | 1.42±0.07 |
The experimental group Egg Quality compares, each experimental group no significant difference.
Table 5, control group and experimental group lace duck early growth comparative trial statistical form (least squares means) unit: gram
The live body of experimental group is heavy, and experimental group YHA is the heaviest in 5,6,7 ages in week, and Zhou Zengchong is the fastest, and feedstuff-meat ratio is placed in the middle.
Table 6, experimental group slaughter determining unit: gram, %, mm
By 6,7 ages in week butcher experiment, each experimental group chest muscle rate, leg flesh rate, the thick difference of sebum are obvious.Experimental group YHA chest muscle rate, leg flesh rate be significantly higher than experimental group AYH, abdomen fat rate no significant difference between each experimental group.
Table 7, (2Kg left and right) productivity comparison when experimental group seed selection group reaches market weight
| Test combinations | Go on the market a day age | Survival rate (%) | Counterpoise (jin) | Feedstuff-meat ratio | Expenses for medicine (unit) |
| YHB | 53 | 95.14 | 4.143 | 3.36∶1 | 0.44 |
| BYH | 50 | 96.29 | 4.029 | 2.91∶1 | 0.35 |
| YHA | 45 | 97.81 | 3.884 | 2.72∶1 | 0.33 |
| AYH | 42 | 90.91 | 3.959 | 2.18∶1 | 0.32 |
Experimental group AYH listing age in days the earliest, and is all low compared with other experimental group feedstuff-meat ratios, expenses for medicine, but survival rate is lower.Experimental group YHA listing age in days is morning, and survival rate is high, and material is than descending, and expenses for medicine reduces.
Routine for appearance is selected and the early stage seed selection of molecule, by the above-mentioned production of hybrid seeds, experimental group YHA hair color occurred with YHB and has separated, a most of duck head and afterbody have some black plumages, and wherein the duck of YHA combination only all has some black plumages at head and afterbody, then based on the reproductive performance to each experimental group, Egg Quality, Slaughter and performance test have finally been established YHA and have been optimum experiment combination, the i.e. production of hybrid seeds pattern of " 2 flowers end to end " lace duck.
Table 8, " 2 flowers end to end " lace duck different lines grow and food consumption
" 2 flowers end to end " " the lace duck under normal feeding and management condition, adopt the controlled feeding technology, finishing period grows normally, build, body weight meet kind of use and require.The finishing period feed consumption hangs down 18.0Kg, cultivates cost lower.From duckling to the adult duck moult time be in addition after 3 weeks (21 days), moult was completed in lasting 19-70 days, mean that the words grade at least of proceeding the outward appearance seed selection is after 40-91 days, and adopt method seed selection of the present invention, a week just begins seed selection after the duck hatching, has shortened 33-81 days, has greatly saved feeding cost, immunity prevents, artificial and place rent is with cost.
In sum, cultivated into paternal 1, maternal 1 of " 2 flowers " lace duck of differing from one another by producing method for seed of the present invention.Under the controlled feeding condition, each strain is along with the generation seed selection improves year by year, and it is consistent that macroscopic features reaches.And institute laciness duck has a common feature-" 2 flowers ", and rostral-caudal respectively has place's black plumage, and this duck is only few at the finishing period feed consumption, cultivates cost low, and egg production is high." 2 flowers " lace duck have a higher heterosis, hybrid vigor, early growth has a clear superiority in." 2 flowers " lace duck has good Slaughter, complete clean thorax 73.5% during 7 age in week, and sebum is thick and abdomen fat rate is low than the Growth of Cherry Valley commercial generation, and viscera tissue physically well develops, and meets southwest consumer's consumption habit, and can cut apart deep processing production." 2 flowers " lace duck breeding technique combines outward appearance seed selection and molecule seed selection, has advance, practicality and operability.Finally determined " 2 flowers end to end " lace duck production of hybrid seeds pattern: see accompanying drawing 1.
The present invention has following characteristics: 1), first the physiological characteristic of lace duck varieties, vitality, breediness etc. are studied, and carry out the breed breeding of system, science, form sign-" 2 flowers end to end " of lace duck new varieties.2) save time and money, if conventional outward appearance seed selection, at least (the general duck that needs 8 years, open and produce age in days more than 120 days, add that collection kind of the egg time, (general 15 days a collection of, each lot number amount is less), added every a collection of hatching number of days 28 days, carried out the outward appearance seed selection in 40-90 days after hatching, generally completing a conventional herd breeding needs about 1 year, then counts in reality seasonal variation factor in operation in, can utilize site factors, and management factors, 8 years general energy seed selection 5 times), what this method seed selection was total has only spent 5 years; The binding molecule seed selection need not wait duck to grow up, and only need to adopt a blood in rear 7 days in birth, extracts the DNA-PCR-order-checking, comparison, these experiments just can be completed in 3 days, satisfactory reserving seed for planting, undesirable eliminating, the duck of eliminating only need not be raised again, directly can sell the saving cost; Secondly, early stage seed selection (be born and just began afterwards in 7 days) has reduced breeding expenses, feed cost, place rent with expense, immune defense expense and labour cost etc.; Due to the seed selection time that shortens, be shorten-originally to wait duck to grow up the generation interval, observe plumage look separation case about 50 days, now if about 10 days just can, and can eliminate undesirablely, ceaselessly hatch simultaneously, ceaselessly seed selection, cultivation that need not be conventional-every a collection of will arriving about 50 days selected again, need not consider every a collection of duck feeding cost only, space, place that takies etc.3) accuracy, if only utilize the molecule seed selection, because feather color probably is subject to the control of a plurality of genes simultaneously, and being difficult to now determine is those several genes, the present invention combines conventional outward appearance seed selection, carry out again the molecule seed selection on the basis of outward appearance seed selection, obtain that appearance is consistent, heritability is stable, maternal lay heavily, paternal early growth meat duck new lines faster.
Claims (3)
1. the lace duck strain producing method for seed with 2 flowers, is characterized in that, comprises the following steps:
1) select the Sichuan lace duck that forms take Bai Yu self-sufficient and strategically located region meat duck drake and Sichuan sheldrake duck selection cross as material, from lace duck colony selective body great in 2kg, the bodily form is wide, neck is thick, 100 of fully-fledged individual drakes are set up into paternal underlying group with 700 of ducks; From opening the lace duck colony of product, next group select the bodily form 130 of individual drakes good, that full-fledged, black wool is few to set up into maternal underlying group with 900 of ducks, two generations of the inbreeding production of hybrid seeds;
2) in the Sichuan lace duck seed selection group who obtains through step 1), selective body is great, the bodily form is wide, neck is thick, fully-fledged drake, again Sichuan lace duck seed selection group's drake and the duck hybridization of cherry valley duck are produced hybridization lace duck, hybridization lace duck carries out outward appearance again and selects the three generations, rejects numb plumage and Bai Yu individuality; Produce hybridization lace duck F1 generation;
3) molecular labeling that utilizes the MC1R gene is to through step 2) hybridization lace duck F1 generation of obtaining chooses seeds in early days, and according to molecular labeling seed selection result and hatching constantly the phenotype result select the individuality of reserving seed for planting, described early stage seed selection comprises the following steps:
(1) hybridization lace duck F1 was for rear 7 days of birth, and venous blood 1mL is adopted at the place at Duck wing; Draw blood 20 μ L utilize phenol/chloroform recovery method to extract DNA;
(2) utilize MC1R gene magnification primer to carry out pcr amplification, template is the DNA sample in hybridization lace duck F1 generation of extracting by step (1), and the amplification total length is 754bp; Check order, the nucleotide sequence that acquisition is increased, described MC1R gene magnification primer is: upstream primer is 5 '-GCCCAATGAACTCTTCCTCACG-3 ', and downstream primer is 5 '-GCACCGAGTTGCAGATGATGAG-3 ';
(3) according to the nucleotide sequence that increases, select to satisfy simultaneously in 411 CC of place genotype and the 756 genotypic individualities of the GG of place to reserve seed for planting, all the other individual eliminating;
4) will set up the family group in breeding objective with the male and female ratio of 1:3 respectively by the individuality of reserving seed for planting that step 3) is obtained and carry out the family breeding, and seal and breed generation F2 generation;
5) will hybridize with white star duck difficult to understand or Beijing duck for individual by the F2 that step 4) obtains, and produce head and afterbody and all have a small amount of black plumage, and health remainder major part be the lace duck of white.
2. the lace duck strain producing method for seed with 2 flowers according to claim 1, it is characterized in that, in step (2): when carrying out pcr amplification, the PCR reaction system is 30 μ L, the full genomic templates DNA that comprises 3 μ L, 2 * Taq Master Mix of 15 μ L, the downstream primer of the upstream primer of 1 μ L and 1 μ L, the ddH of 10 μ L
2O； Amplified reaction is all completed on the PCR instrument.
3. the lace duck strain producing method for seed with 2 flowers according to claim 2, is characterized in that, the PCR reaction condition is as follows:
(a) 95 ℃ of denaturation 4min;
(b) 94 ℃ of sex change 30 s;
(c) 53.5 ℃ of 45s of annealing temperature;
(d) 72 ℃ are extended 50s;
(e) step (b)-(d) repeats 35 times;
(f) 72 ℃ are extended 15min.
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| CN102783456B (en) * | 2012-08-31 | 2013-10-16 | 贵州千里山生态食品股份有限公司 | Method for modifying hybrid matching strains of Sansui duck |
| CN103478073A (en) * | 2013-09-06 | 2014-01-01 | 四川农业大学 | Production method of black-feather meat duck |
| CN104126543B (en) * | 2014-07-18 | 2017-04-19 | 周黑鸭食品股份有限公司 | Hybrid seed production method of meat ducks with light black beaks, black feet and black feathers |
| CN105284733B (en) * | 2015-11-20 | 2017-12-26 | 中国农业科学院北京畜牧兽医研究所 | A kind of white plumage generic cross duck female parent selection |
| CN111334588B (en) * | 2020-04-20 | 2022-07-22 | 四川农业大学 | Molecular marker related to black and brown feather characters of duck and application thereof |
| CN112021256B (en) * | 2020-08-24 | 2022-02-11 | 大余马蹄山生态农业开发有限公司 | Breeding method of pure breed sheldrake |
| CN116918763A (en) * | 2023-08-29 | 2023-10-24 | 山东樱桃谷农业科技有限公司 | Breeding method of ducks |
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| CN1669414A (en) * | 2005-04-13 | 2005-09-21 | 江苏高邮鸭集团 | Hybridization complement technique for making ducks lay eggs with greenish shell |
| CN101904318A (en) * | 2010-08-09 | 2010-12-08 | 镇江市天成畜禽有限公司 | Breeding method of ornamental tufted ducks |
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|---|---|---|---|---|
| CN1669414A (en) * | 2005-04-13 | 2005-09-21 | 江苏高邮鸭集团 | Hybridization complement technique for making ducks lay eggs with greenish shell |
| CN101904318A (en) * | 2010-08-09 | 2010-12-08 | 镇江市天成畜禽有限公司 | Breeding method of ornamental tufted ducks |
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| 建昌鸭的羽色类型及其主要经济性状;曾凡同等;《中国畜牧杂志》;19891231;第25卷(第3期);第28-30页 * |
| 曾凡同等.建昌鸭的羽色类型及其主要经济性状.《中国畜牧杂志》.1989,第25卷(第3期),第28-30页. |
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