CN105340713B - A kind of organic strawberry tissue-culture container seedling transplanting medium and method for culturing seedlings - Google Patents
A kind of organic strawberry tissue-culture container seedling transplanting medium and method for culturing seedlings Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The present invention disclose a kind of organic strawberry tissue-culture container seedling transplanting medium and its method for culturing seedlings, it includes tea seed cake fermentation material, turf, vermiculite and perlite, the tea seed cake fermentation material, turf, vermiculite and perlite volume ratio be 0.2~0.4：0.6~0.8：0.5：0.5.The tea seed cake fermentation material is prepared using following steps：(1) tea seed cake powder is broken into tea seed powder and mixture is made with sawdust mixing, simultaneously mixing is uniformly sprayed on mixture after EM stostes are diluted with water, adjust carbon/nitrogen ratio, pH value and water content of mixture；(2) it builds heap fermentation and tea seed cake fermentation material is made.The seedling medium cooperation special nutrient fluid that the present invention is configured to, the seedling brought out is compared with generic media nursery：Survival rate, planting percent improve；The diseases such as anthrax, root-rot can be effectively controlled, strawberry dark green leaf color, radical increase, and maternal plant is healthy and strong；Without using root agent, antibiotic and inorganic nutrient solution is lured in seedling raising process, seedling raising process realizes organic control.
Description
Technical field：
The present invention relates to field of agricultural cultivation more particularly to a kind of organic strawberry tissue-culture container seedling transplanting medium and nursery sides
Method.
Technical background：
Strawberry is become with it higher than the vitamin content of other 10 times of fruit and distinctive ellagic acid with anticancer effect
Most popular health fruit, country of the China as cultivated strawberry area maximum, cultivated area is more than 70,000 hectares, yield
Up to 700,000 tons.And the enhancing with health care consciousness with the improvement of people ' s living standards, the organic strawberry market demand of reply
Constantly increase, also proposed Strawberry Seedlings the requirement of " organic ".In current cultivated strawberry, strawberry nursery is as realization high yield
Important measures, researcher constantly improve strawberry seedling-raising technique, currently with tissue culture technique culture virus-free strawberry bottle seedling,
And it carries out rooting induction using Light media and is used widely in production with domestication acquisition strawberry maternal plant technology.Bottle seedling transplants base
Raw material is mainly sawdust, coconut palm chaff, bacteria residue, peat, earthworm excrement etc. and adds in the nothings such as perlite, vermiculite by a certain percentage used by matter
Machine matrix is made, by the use of its matrix as bottle seedling transplanting medium, with traditionally using stem breeding ratio of crawling, breeding coefficient, disease resistance
And young plant quality greatly improves.But due to after bottle seedling is transplanted, often due to culture medium does not have wash clean that phytosis can occur
Evil inhibits to take root, and influences to survive, therefore control germ contamination and the root induction such as common carbendazim, penicillin, NAA, improves into
Motility rate.And the later stage also easily infects the diseases such as anthrax, root-rot, it is necessary to be controlled with fungicide such as charcoal Trane, root-rot spirits, the later stage
In order to which robust seedling also needs supplement inorganic nutrient solution, the seedling brought out in this way is unable to reach organic standard.
Tea seed cake is the byproduct after tea seed liquefaction, have very high nutritive value, rich in organic matter, nitrogen, phosphorus, potassium, calcium,
The nutrients such as magnesium, fluorine, manganese, available nitrogen, potassium are multiplied and a kind of good base starting material after decomposition；It is peculiar in tea seed
Tea Saponin, have good fungistatic effect to all kinds of bacteriums, fungi.
Therefore, the feasibility as strawberry seedling medium of tea seed cake is developed, widens the raw material of tissue-culture container seedling transplanting medium
Source, a kind of tissue-culture container seedling transplanting base that can be reduced cost and significantly improve the pre- disease prevention of strawberry survival rate, planting percent of research and development
Matter and nutrient solution are the technical issues of those skilled in the art are urgently to be resolved hurrily.
Invention content：
The purpose of the present invention is to provide a kind of organic strawberry tissue-culture container seedling transplanting mediums.
Another object of the present invention is to provide a kind of organic strawberry tissue-culture container seedling transplanting medium nursery special nutrient fluid.Have
In machine Strawberry Plantlets bottle seedling transplanting medium and seedling raising process dedicated nutrient solution using tea seed cake have abundant nutrient with
Using EM fermentation techniques, tea seed cake is fermented in different ways for the characteristic of the distinctive bacteriostasis of Tea Saponin, and organic grass is made
The fermentation materials of certain kind of berries bottle seedling transplanting medium and organic strawberry tissue-culture container seedling nursery special nutrient fluid；By fermentation materials and common matrix
(turf, vermiculite, perlite) is mixed in a certain ratio, and is configured to organic strawberry tissue-culture container seedling seedling medium.For organic strawberry
Tissue-culture container seedling nursery solves technological deficiency present in technical background.
Another object of the present invention is to provide a kind of organic strawberry tissue-culture container seedling transplanting medium method for culturing seedlings.
The purpose of the present invention is achieved through the following technical solutions：
A kind of organic strawberry tissue-culture container seedling transplanting medium, it includes tea seed cake fermentation material, turf, vermiculite and perlite, institute
The volume ratio for stating tea seed cake fermentation material, turf, vermiculite and perlite is 0.2~0.4：0.6~0.8：0.5：0.5.
As a kind of optimal technical scheme, the tea seed cake fermentation material, turf, vermiculite and perlite volume ratio be 0.3：
0.7：0.5：0.5.
Above-mentioned tea seed cake fermentation material is prepared using following steps：
(1) tea seed cake powder is broken into tea seed powder and mixture is made with sawdust mixing, after EM stostes are diluted with water uniformly
It is sprayed on mixture and mixing, the carbon/nitrogen ratio for adjusting mixture is：25~30：1, pH value is：5.5~6.5, water content is
60%~65%；
(2) heap fermentation is built：The mixture that step (1) obtains is built after heap and covers film and shading, progress aerobic fermentation 3~
It is 5 days, primary every turning in 2 days, tea seed cake fermentation material is made.
As a kind of optimal technical scheme, the amount ratio of the tea seed powder and sawdust is：Add 40 in per 1000kg tea seed powder
~60kg sawdust mixings.
The EM stostes and the amount ratio of tea seed powder are：2~3kgEM stostes are used per 1000kg tea seeds powder.
It is further preferred that the high 0.4~0.5m of heap, heap wide 1~1.2m when the mixture builds heap.
A kind of organic strawberry tissue-culture container seedling transplanting medium nursery special nutrient fluid, is prepared using following steps：
(1) tea seed cake powder is broken into tea seed powder and mixture is made with sawdust mixing, after EM stostes are diluted with water uniformly
It is sprayed on mixture and mixing, the carbon/nitrogen ratio for adjusting mixture is：25~30：1, pH value is：5.5~6.5, water content is
60%~65%；
(2) fermentation material 1 and fermentation material 2 are prepared：Film and shading are covered after the mixture that step (1) obtains is built heap, into
It acts charitably aerobe fermentation 3~5 days, it is primary every turning in 2 days, tea seed cake fermentation material 1 is made；The mixture that step (1) obtains is built into heap
Covering film and shading afterwards, carry out aerobic fermentation 15~20 days, and every turning in 5~7 days is primary, and tea seed cake fermentation material 2 is made；
(3) it prepares mixed fermentation material and carries out compounding：Fermentation material 2 prepared by step (2) and fermentation material 1 are pressed into matter
Amount is than being 80~90：Mixed fermentation material is made in 10~20 ratio mixing, is then 1 in mass ratio by mixed fermentation material and water：
8~9 mixings are put into fermentation filling, and after supplementing EM stostes, anaerobic fermentation 2~3 days obtains zymotic fluid, filtering fermentation liquor is obtained
Organic strawberry tissue-culture container seedling transplanting medium nursery special nutrient fluid.
Above-mentioned machine Strawberry Plantlets bottle seedling nursery special nutrient fluid pours applying method：To during seedling after tissue-cultured seedling survives, incite somebody to action
20~25 times of special nutrient fluid dilution obtained, which is poured, to be applied 2~3 times.
As a kind of optimal technical scheme, the amount ratio of tea seed powder and sawdust is described in step (1)：Per 1000kg tea seeds
Add 40~60kg sawdust mixings in powder；
The EM stostes and the amount ratio of tea seed powder are：2~3kgEM stostes are used per 1000kg tea seeds powder；In step (2)
High 0.4~the 0.5m of heap, heap wide 1~1.2m when the mixture builds heap；
The additive amount of EM stostes described in step (3) adds 0.5~1kgEM stostes for 100kg water.
A kind of organic strawberry tissue-culture container seedling transplanting medium method for culturing seedlings, using above-mentioned transplanting medium as organic strawberry tissue culture
To during seedling after Strawberry Plantlets bottle seedling transplant survival, above-mentioned organic strawberry tissue-culture container seedling is transplanted for bottle seedling transplanting medium
20~25 times of substrate seedling special nutrient fluid dilution, which is poured, to be applied 2~3 times.
The Strawberry Plantlets bottle seedling transplanting medium physicochemical character such as table 1 prepared in above-mentioned technical proposal.
1 organic strawberry tissue-culture container seedling transplanting medium physicochemical character of table
The matrix retentiveness, aeration are good, containing a certain amount of organic matter, nutrient, beneficial bacterium and Tea Saponin, soda acid
It spends moderate, can effectively inhibit all kinds of bacteriums, fungi, promote the control of organic strawberry rooting and disease.
The tea seed cake is the byproduct after conventional organic tea seed liquefaction, and it is 10%~20% to air-dry to water content.Institute
The sawdust stated is timber processing byproduct, is air-dried to water content 10%~20%.
The EM stostes are provided by Nanjing Ai Mule environment friendly biologicals Technology Co., Ltd..It is a kind of NEW TYPE OF COMPOSITE microorganism
It is common to belong to more than 80 kind microorganisms by 5 sections 10 such as photosynthetic bacteria group, lactobacillus, yeast flora, actinomycete group, Filamentous floras for microbial inoculum
Composition.
The present invention is added in using nutriment and the bacteriostasis of distinctive Tea Saponin in tea seed cake in conventional substrate
A certain amount of tea seed cake fermentation material, is made Strawberry Plantlets bottle seedling transplanting medium, in Strawberry Seedlings growth course, is made using tea seed cake
Special nutrient fluid, which pours, to be applied, and use existing for existing Strawberry Plantlets bottle seedling transplanting medium can be overcome to lure root agent, antibiotic, sterilization
Agent, inorganic nutrient solution etc. can not realize the defects of organic seedling, ensure the survival rate of strawberry, nurturing staff.
Beneficial effects of the present invention：
The organic strawberry tissue-culture container seedling seedling medium that the present invention is configured to is rich in organic matter, various nutriments, containing having greatly
A certain amount of beneficial bacterium, Tea Saponin etc. for transplanting organic strawberry tissue-culture container seedling, mix organic strawberry group again in seedling raising process
Bottle seedling special nutrient fluid is trained, the seedling brought out is compared with generic media nursery：Survival rate, planting percent improve；Can effectively control anthrax,
The diseases such as root-rot, strawberry dark green leaf color, radical increase, and maternal plant is healthy and strong；Without using luring root agent, antibiotic and inorganic in seedling raising process
Nutrient solution, seedling raising process realize organic control.
Specific embodiment：
Embodiment 1：Organic strawberry tissue-culture container seedling transplanting medium is prepared
(1) tea seed cake powder is broken into tea seed powder, and mixture is made by tea seed powder 1000kg, sawdust 50kg mixings；By EM
Stoste 3kg is dissolved in 450kg water to be uniformly sprayed on mixture after dilution, is turned in spray, the C/N ratios for adjusting mixture are：25
~30：1, pH value is：5.5~6.5, water content is 60%~65%.After the tea seed cake is conventional organic tea seed liquefaction
Byproduct, it is 10%~20% to air-dry to water content.The sawdust be timber processing byproduct, air-dry to water content 10%~
20% (similarly hereinafter).
(2) heap fermentation is built：After the heap that above-mentioned mixture is piled to high 0.4~0.5m, wide about 1~1.2m, plastics in covering
Film with the appropriate shading such as straw screen or mat, carries out aerobic fermentation 3 days, 5 days, 7 days, 10 days, every turning one in 2 days in fermentation process respectively
It is secondary, 4 kinds of tea seed cake fermentation materials are made.
Respectively above 4 kinds of tea seed cake fermentation materials mix matching by three kinds of volume ratios respectively with peat, vermiculite, perlite
It is formed with machine Strawberry Plantlets bottle seedling transplanting medium：
Proportioning 1：0.2：0.8：0.5：0.5
Proportioning 2：0.3：0.7：0.5：0.5
Proportioning 3：0.4：0.6：0.5：0.5
With peat, vermiculite, perlite by volume for 1：0.5：0.5 is control, sets 13 processing altogether, and complex treatment is set
It puts and is shown in Table 2.
By in the matrix mixed in the seedlings nursing plate of 50 cavities, and by the group with 2~3 blades, 2~3cm of height of seedling
The culture medium of training bottle seedling root is cleaned, and is transplanted into hole tray.The fermentation material of different fermentations number of days is sampled into detection before matrix compounding
It analyzes its EC value and Tea Saponin content, testing result is shown in Table 3；When Strawberry Seedlings grow young leaves, its survival rate, new radical are carried out
Investigation；Inorganic nutrient solution is supplemented, until during seedling, its planting percent, root rot, anthracnose incidence are investigated, investigation result
It is shown in Table 4.Determine the optimum compound proportion example of the most suitable fermentation number of days of tea seed cake and organic strawberry tissue-culture container seedling transplanting medium.
2 organic strawberry tissue-culture container seedling transplanting medium complex treatment of table is set
The variation of Tea Saponin and EC values in 3 different fermentations number of days tea seed cake of table
4 different disposal Strawberry Plantlets bottle seedling survival rate of table, the amount of taking root, planting percent, root rot, anthracnose incidence
Table 3 the result shows that：With the increase of tea seed cake fermentation number of days, Tea Saponin content gradually decreases, and EC values are gradually increased.
During fermentation 3~5 days, Tea Saponin reduces and the raising unobvious of EC values, when fermenting between when reaching 7~10 days, Tea Saponin content
Decline and the raising of EC values are clearly.Therefore 3~5 days ferment effects that ferment are best.
Table 4 the result shows that：The matrix that compounds after 1~6 processing group, i.e. tea seed cake are fermented 3~5 days, organic strawberry bottle seedling into
Motility rate 78%~84% is approached with compareing 82%, 4.1~4.5 piece/plant of the amount of taking root, and than control, 4.2 pieces/plant are slightly good, planting percent
75%~79%, it increases than control 72%；Root rot incidence 3.8%~6.1% is decreased obviously than control 12.2%；Charcoal
Subcutaneous ulcer disease incidence 4.0%~5.3% is decreased obviously than control 11.3%.7~12 processing groups, that is, tea seed cake is multiple after fermenting 7~10 days
The matrix matched, strawberry survival rate 63%~75%, 2.8~3.6 piece/plant of the amount of taking root, planting percent 57%~71% are below compareing,
Root rot (5.3%~10%) anthracnose (8.2%~8.8%) is less than control group, higher than 1~6 processing group.This is because tea seed
Cake extends fermentation time, and the Tea Saponin content in tea seed cake declines and EC values improve, and fungistatic effect declines, and influences strawberry root of hair,
Reduce the survival rate and disease resistance of strawberry.
As a result also show：In 1~6 processing group, the items such as survival rate, the amount of taking root, planting percent, root rot, anthracnose refer to
Mark is using the 2nd processing group and the 5th processing group to be excellent.
To sum up analyze：Tea cake fermentation number of days was advisable with 3~5 days, tea seed cake fermentation material and turf, vermiculite, the pearl of gained
Rock is 0.2~0.4 by volume：0.8~0.6：0.5：0.5 conveniently, and most preferably 0.3：0.7：0.5：0.5.
The fermentation side of tea seed cake fermentation material 2 in 2 organic strawberry tissue-culture container seedling transplanting medium nursery special nutrient fluid of embodiment
Method
Tea seed cake powder is broken into tea seed powder, and mixture is made by tea seed powder 1000kg, sawdust 50kg mixings；By EM stostes
3kg is dissolved in 450kg water to be uniformly sprayed on mixture after dilution, is turned in spray, the C/N ratios for adjusting mixture are：25~30：
1, pH value is：5.5~6.5, water content is 60%~65%.Mixture is piled to the heap of high 0.4~0.5m, wide about 1~1.2m
Afterwards, plastic foil in covering with the appropriate shading such as straw screen or mat, ferments 15 days, 20 days, 25 days, sets 3 processing altogether, fermenting respectively
Cheng Zhong, it is primary every turning in 5 days, after fermentation, the situation of change of quick-acting nutrients in fermentation materials is measured, testing result is shown in Table
5, determine its number of days that ferments.
The variation of 5 tea seed cake different fermentations number of days available nutrient of table
Table 5 the result shows that, when ferment 15~20 days when, available N is gradually increased with longer fermentation times, available P2O5、
K2O also reaches certain high point；Continue to ferment after 20 days, available N has downward trend, available P2O5、K2O is increased slightly, but is increased
Unobvious.Therefore, from the validity and the angle of reduction nitrogen loss for ensuring tea seed cake nutrient, tea seed cake fermentation 15~20
It is more suitable.
The preparation of 3 organic strawberry tissue-culture container seedling transplanting medium nursery special nutrient fluid of embodiment and effect
Tea seed cake powder is broken into tea seed powder, and mixture is made by tea seed powder 1000kg, sawdust 50kg mixings；By EM stostes
3kg is dissolved in 450kg water to be uniformly sprayed on mixture after dilution, is turned in spray, the C/N ratios for adjusting mixture are：25~30：
1, pH value is：5.5~6.5, water content is 60%~65%；
After the heap that mixture is piled to high 0.4~0.5m, wide about 1~1.2m, plastic foil in covering is suitably hidden with straw screen or mat etc.
Light, carries out aerobic fermentation 3 days, primary every turning in 2 days, and tea seed cake fermentation material 1 is made；
After the dunghill that mixture is piled to high 0.4~0.5m, wide about 1~1.2m, plastic foil in covering is appropriate with straw screen or mat etc.
Shading, carries out aerobic fermentation 20 days, primary every turning in 5 days, and tea seed cake fermentation material 2 is made；
Respectively by tea seed cake fermentation material 2 and tea seed cake fermentation material 1 in mass ratio 100：0、90：10、85：15 and 80：20 is mixed
Even obtained mixed fermentation material, sets 4 processing groups altogether, is respectively 1 in mass ratio by the mixed fermentation material of 4 processing groups and clear water：8
Mixing (floods, while ensures a certain concentration of nutrient solution again) to greatest extent, is put into fermentation filling, appropriate to supplement EM bacterium, mends
Charge adds 0.8kg EM stostes by 100kg water, and closing anaerobic ferments 3 days, nutritious fermentation liquor is obtained, by the filtering fermentation liquor of gained
Obtain the special nutrient fluid in organic strawberry tissue-culture container seedling transplanting medium seedling raising process, by special nutrient fluid dilute 25 times respectively at
Pouring altogether for 15 days, 25 days after the transplanting of strawberry bottle seedling applies 2 times, and (strawberry transplanting medium is preferred organic strawberry tissue culture in embodiment 1
Bottle seedling transplanting medium, that is, processing group 2), if 2 controls, CK1 strawberries transplanting medium is preferred organic strawberry tissue culture in embodiment 1
Bottle seedling transplanting medium, that is, processing group 2, does not pour nutrient solution, and CK2 strawberry transplanting mediums are peat, vermiculite, perlite are by volume
1：0.5：0.5, pour conventional inorganic nutrient solution and positive common medicine.Measured after seedling its strawberry leaves the piece number, plant height, root dry weight,
Top dry weight, anthracnose, root rot incidence.Testing result is shown in Table 6.Determine the compound proportion of fermentation material.
6 different disposal Strawberry Seedlings growth of table, seedling, incidence
Table 6 the result shows that, using the organic strawberry tissue-culture container seedling transplanting medium nursery of preparation, pour the processing (17 of nutrient solution
~20) compare with CK1 (21), dark green leaf color, plant height, the number of blade of strawberry, overground part dry weight and root dry weight significantly increase, planting percent
Also it significantly improves, the incidence reduction of root-rot, anthracnose, and part index number is also slightly better than CK2.Pour the processing (17 of nutrient solution
~20) between, difference is little on leaf color, the number of blade, overground part dry weight, root dry weight, planting percent, but from the point of view of strawberry incidence,
It is applied alone (processing 17) root rot of nutrient solution made from fermentation material 2 and anthracnose and CK1 differences little, and with fermentation material 2 with fermenting
Material 1 in mass ratio 90：10、85：15、80：Nutrient solution, root rot and anthracnose and CK1 are poor made from 20 ratio mixing fermentation
Different apparent, root rot controls substantially, and anthracnose is entirely capable of controlling.
To sum up analyze：Fermentation material 2 is with 1 mixed proportion of fermentation material using mass ratio as 90~80：10~20 mixing, in strawberry group
It is advisable after training bottle seedling transplant survival to pouring 2 times during seedling, it is proposed that poured again before young plant is out of the garden 1 time.
Claims (6)
1. a kind of organic strawberry tissue-culture container seedling transplants nursery special nutrient fluid, it is characterised in that is prepared using following steps：
（1）Tea seed cake powder is broken into tea seed powder and mixture is made with sawdust mixing, uniformly sprinkling after EM stostes are diluted with water
On mixture and mixing, the carbon/nitrogen ratio for adjusting mixture are：25~30：1, pH value is：5.5~6.5, water content for 60%~
65%；
（2）Prepare fermentation material 1 and fermentation material 2：By step（1）Obtained mixture builds covering film and shading after heap, into acting charitably
Aerobe fermentation 3~5 days, it is primary every turning in 2 days, tea seed cake fermentation material 1 is made；By step（1）Obtained mixture covers after building heap
Lid film and shading, carry out aerobic fermentation 15~20 days, and every turning in 5~7 days is primary, and tea seed cake fermentation material 2 is made；
（3）It prepares mixed fermentation material and carries out compounding：By step（2）The fermentation material 2 and fermentation material 1 of preparation are in mass ratio
It is 80~90：Mixed fermentation material is made in 10~20 ratio mixing, is then 1 in mass ratio by mixed fermentation material and water：8~9
Mixing is put into fermentation filling, and after supplementing EM stostes, anaerobic fermentation 2~3 days obtains zymotic fluid, filtering fermentation liquor obtained organic
Strawberry Plantlets bottle seedling transplants nursery special nutrient fluid.
2. organic strawberry tissue-culture container seedling according to claim 1 transplants nursery special nutrient fluid, it is characterised in that step（1）
Described in the amount ratio of tea seed powder and sawdust be：Add 40~60kg sawdust mixings in per 1000kg tea seed powder；The EM stostes with
The amount ratio of tea seed powder is：2~3kgEM stostes are used per 1000kg tea seeds powder；Step（2）Described in mixture when building heap heap it is high
Wide 1~the 1.2m of 0.4~0.5m, heap；Step（3）Described in the additive amounts of EM stostes add 0.5~1kgEM stostes for 100kg water.
3. a kind of organic strawberry tissue-culture container seedling transplants method for culturing seedlings, which is characterized in that transplants base using organic strawberry tissue-culture container seedling
To during seedling after Strawberry Plantlets bottle seedling transplant survival, organic strawberry tissue-culture container seedling described in claim 1 transplanting is educated for matter
20~25 times of seedling special nutrient fluid dilution, which is poured, to be applied 2~3 times；
The organic strawberry tissue-culture container seedling transplanting medium includes tea seed cake fermentation material, turf, vermiculite and perlite, the tea seed
Cake fermentation material, turf, vermiculite and perlite volume ratio be 0.2 ~ 0.4：0.6~0.8：0.5：0.5；
The tea seed cake fermentation material is prepared using following steps：
（1）Tea seed cake powder is broken into tea seed powder and mixture is made with sawdust mixing, uniformly sprinkling after EM stostes are diluted with water
On mixture and mixing, the carbon/nitrogen ratio for adjusting mixture are：25~30：1, pH value is：5.5~6.5, water content for 60%~
65%；
（2）Build heap fermentation：By step（1）Obtained mixture builds covering film and shading after heap, carries out aerobic fermentation 3~5 days,
It is primary every turning in 2 days, tea seed cake fermentation material is made.
4. organic strawberry tissue-culture container seedling according to claim 3 transplants method for culturing seedlings, it is characterised in that the tea seed cake hair
Ferment material, turf, vermiculite and perlite volume ratio be 0.3：0.7：0.5：0.5.
5. organic strawberry tissue-culture container seedling according to claim 3 transplants method for culturing seedlings, it is characterised in that the tea seed powder with
The amount ratio of sawdust is：Add 40~60kg sawdust mixings in per 1000kg tea seed powder；The EM stostes and the amount ratio of tea seed powder
For：2~3kgEM stostes are used per 1000kg tea seeds powder.
6. organic strawberry tissue-culture container seedling according to claim 3 transplants method for culturing seedlings, it is characterised in that the mixture is built
High 0.4~the 0.5m of heap, heap wide 1~1.2m during heap.
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| CN107188674B (en) * | 2017-06-01 | 2020-11-06 | 河海大学 | Solanaceous vegetable seedling culture substrate and preparation method thereof |
| CN109197509A (en) * | 2018-10-22 | 2019-01-15 | 商丘市农林科学院 | A kind of half matrix of strawberry |
| CN111296259A (en) * | 2020-02-26 | 2020-06-19 | 河北省农林科学院石家庄果树研究所 | Cultivation method for preventing and treating strawberry anthracnose |
| CN111543230B (en) * | 2020-03-30 | 2021-09-14 | 同济大学 | Large-scale blueberry planting method suitable for mountainous regions |
| CN113455319A (en) * | 2021-06-21 | 2021-10-01 | 百色学院 | Transplanting method of cassava tissue culture contaminated seedlings |
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