CN105326898A - Compound immunopotentiator for apostichopus japonicus - Google Patents
Compound immunopotentiator for apostichopus japonicus Download PDFInfo
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- CN105326898A CN105326898A CN201510685091.1A CN201510685091A CN105326898A CN 105326898 A CN105326898 A CN 105326898A CN 201510685091 A CN201510685091 A CN 201510685091A CN 105326898 A CN105326898 A CN 105326898A
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- Prior art keywords
- apostichopus japonicus
- gluconate
- radix sophorae
- sophorae flavescentis
- herba taraxaci
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/288—Taraxacum (dandelion)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/30—Zinc; Compounds thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/32—Manganese; Compounds thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Life Sciences & Earth Sciences (AREA)
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Abstract
The invention discloses a compound immunopotentiator for apostichopus japonicus. The compound immunopotentiator comprises, by weight, 1-5% of zinc gluconate, 45-49% of manganese gluconate, 20-30% of herba taraxaci and 20-30% of radix sophorae flavescentis. A preparation method of the compound immunopotentiator includes decocting the herba taraxaci and the radix sophorae flavescentis in 50 times amount of water, discarding decoction dregs, dissolving the mineral elements of the zinc gluconate and the manganese gluconate into traditional Chinese medicine decoction, and splashing the compound immunopotentiator into aquaculture water according to the 0.1g/L dose. The compound immunopotentiator for the apostichopus japonicus has the advantages of scientific and reasonable formula, quick action, capability of improving apostichopus japonicus immunity remarkably, exact ingredients, small dosage, no toxic or side effect on the apostichopus japonicus, avoidance of food and environmental safety problems, stable raw material sources, low price and simplicity and feasibility in operation.
Description
Technical field
The present invention relates to a kind of Stichopus japonicus immunostimulant, be specifically related to a kind of Apostichopus japonicus mineral element and Chinese herbal medicine composite immune reinforcing agent, belong to echinoderm immunological technique field.
Background technology
Apostichopus japonicus belongs to invertebrates Echinodermata, is one of economic kind of the topmost aquaculture in northern China coastal waters.In recent years, due to reasons such as kind of a matter degeneration, cultivation density increase and breeding environment deteriorations, Apostichopus japonicus in nursery, keep a full stand of seedings and the various diseases that takes place frequently in breeding process, as rotten gastropathy, rotten limit disease, skin ulcerative syndrome, acute mouth enclose swelling etc., cause serious economic loss, have impact on the development of Apostichopus japonicus aquaculture.Antibiotics due to the problems such as resistance, food and Environmental security disease control of aquatic animal application on there is significant limitation, therefore, the use of nuisanceless immunostimulant becomes one of topmost means of aquatic animal disease prevention and control.Chinese herbal medicine, as nuisanceless immunostimulant conventional in aquaculture, is widely used in the marine cultured animals such as Stichopus japonicus, Hemicentrotus seu Strongylocentrotus, prawn, Carnis Haliotidis.At present in Apostichopus japonicus, immunostimulant agent prescription is Chinese herb compound or Chinese herbal medicine extract compound recipe mainly, by to throw something and feed or breeding water body is splashed mode dispenser, the immunity of Apostichopus japonicus can be significantly improved, but because Chinese medicine mechanism of action is complicated, Chinese herb compound or Chinese herbal medicine extract compound recipe are improving shortcoming Apostichopus japonicus immunity also existing onset time length.
Summary of the invention
The object of this invention is to provide a kind of Apostichopus japonicus to be had no side effect, nuisanceless, mineral element and the Chinese herbal medicine composite immune reinforcing agent of Apostichopus japonicus immunity can be improved by Fast Persistence, thus make up the deficiencies in the prior art.
Technical solution of the present invention is:
A kind of Apostichopus japonicus composite immune reinforcing agent, is characterized in that comprising following weight percent composition:
Zinc gluconate 1 ~ 5%;
Manganese gluconate 45 ~ 49%;
Herba Taraxaci 20 ~ 30%;
Radix Sophorae Flavescentis 20 ~ 30%.
Further, each component optimum weight percentage ratio is:
Zinc gluconate 2%;
Manganese gluconate 46%;
Herba Taraxaci 25%;
Radix Sophorae Flavescentis 27%.
In the present invention, Herba Taraxaci has effect of broad-spectrum antiseptic and sterilization, also has inhibitory action to some fungus, also significantly can activate the phagocytal vigor of body endocytosis simultaneously; Radix Sophorae Flavescentis, except having effect of Antifungi, antiviral, anti-inflammation, also has immunoregulatory effect.
Except Chinese herbal medicine, some mineral elements also have potential immunostimulant using value in aquatic animal.Among mineral element, manganese and zinc comprise people and the necessary trace element of Apostichopus japonicus many animals, significantly can improve the vigor of some immune relevant enzyme.Such as, manganese has significant activation and facilitation to the superoxide dismutase in animal body, alkali phosphatase, phenol oxidase and nucleic acid polymerase etc.; And zinc plays a significant role in the vigor of interleukin, superoxide dismutase, lysozyme, ATP enzyme etc. keeps and promotes.The inorganic salt of manganese and zinc has certain bio-toxicity effect, and its gluconate inanimate object toxic action, and be used widely in human lives as health product and food additive etc., therefore the present invention selects manganese gluconate and zinc gluconate as the source of mineral element manganese and zinc.In addition, mineral element and Chinese herbal medicine regulate the mechanism of immunity of organisms different, Chinese herbal medicine on the one hand by the vigor of the compositions such as its polysaccharide contained through complicated approach Promote immunity cell and immune relevant enzyme, on the other hand by having sterilization, the organic compound of bacteriostatic activity directly suppresses the Growth and reproduction of pathogen in body; And mineral element participates in maintenance and the adjustment of immunity and metabolizing enzyme vigor in body directly mainly as enzymatic cofactor.Therefore, by suitable mineral element and Chinese herbal medicine with the use of, by number of ways combined effect, improve immunity of organisms sooner, better.
The present invention is a kind of mineral element for improving Apostichopus japonicus immunity and Chinese herbal medicine compound formulation, containing zinc gluconate and manganese gluconate two kinds of mineralogical compositions and Herba Taraxaci and Radix Sophorae Flavescentis two kinds of Chinese medicine ingredients, its preparation method is after Herba Taraxaci and Radix Sophorae Flavescentis two kinds of Chinese medicine ingredients are added 50 times of soak by water, discard medicinal residues, again zinc gluconate and manganese gluconate two kinds of mineralogical compositions are dissolved in decocting for Chinese herbal medicine liquid, then splash in breeding water body according to the dose of 0.1g/L.
The invention has the advantages that: scientific formulation is reasonable, rapid-action, can significantly improve Apostichopus japonicus immunity; Definite ingredients, using dosage is little, has no side effect to Apostichopus japonicus, can not produce food and environmental security; Raw materials enjoy stable sources, cheap, operation is simple.
Accompanying drawing explanation
Fig. 1 is the impact of composite immune reinforcing agent of the present invention on Apostichopus japonicus Acid Phosphatase Activity.
Fig. 2 is the impact of composite immune reinforcing agent of the present invention on Apostichopus japonicus alkaline phosphatase activity.
Fig. 3 is the impact of composite immune reinforcing agent of the present invention on Apostichopus japonicus superoxide dismutase activity.
Fig. 4 is the impact of composite immune reinforcing agent of the present invention on Apostichopus japonicus antalzyme activity.
Fig. 5 is the impact of composite immune reinforcing agent of the present invention on Apostichopus japonicus Phenoloxidase Activities.
Detailed description of the invention
The present invention is further illustrated by specific embodiment below in conjunction with accompanying drawing.
Healthy Apostichopus japonicus used totally 100 in following embodiment, take from Research Institute of Ocean Fishery Science, Liaoning Province and draw breeding center, body weight 21 ± 3.3g, on average support temporarily in 4 100L tanks (containing 80L sea water, water temperature 16 ~ 18 DEG C, pH8.2 ~ 8.5, salinity 28), 1. tank numbers respectively, 2., 3., 4..Apostichopus japonicus is supported temporarily and is added composite immune reinforcing agent after one week.
Embodiment 1:
Get Herba Taraxaci 20g, Radix Sophorae Flavescentis 30g, after adding 2500g water boil half an hour, abandon medicinal residues, zinc gluconate 1g, manganese gluconate 49g are dissolved in decoction liquor, be prepared into immunostimulant and use liquid.
The immunostimulant of above-mentioned preparation is splashed in screw clamp breeding water body 1. with the amount of 0.1g/L, supports 20 days temporarily subsequently.Period changes weekly water 1 time and supplements immunostimulant to 0.1g/L.After interpolation immunostimulant, the 5th day, 10 days and 20 days difference random selecting, 6 Apostichopus japonicus, cut abdominal cavity open and obtain coelomic fluid.By with centrifugal after the coelomic fluid mixing of batch 6 Apostichopus japonicus, get supernatant, measure the vigor of immune relevant enzyme in coelomic fluid supernatant.4. tank is used as reference examples, does not add immunostimulant, and all the other operations are with adding immunostimulant.
Acid Phosphatase Activity and alkaline phosphatase activity adopt " Nanjing is built up " disodium phenyl phosphate method kit measurement, superoxide dismutase activity adopts " Nanjing is built up " WST-1 method kit measurement, antalzyme activity adopts " Nanjing is built up " turbidimetry kit measurement, protein concentration adopts " Nanjing is built up " BCA method kit measurement, and concrete determination step carries out according to test kit description.
The assay method of Phenoloxidase Activities is as follows: after 100 μ L Apostichopus japonicus coelomic fluid supernatants and 2.0mL20mmol/L levodopa solution mix, under 492nm wavelength, measure light absorption value, surveys once, survey 10 times every 3min.
a 490increase by 0.001 per minute is defined as 1 unit of activity (U).
Enzyme activity assay experiment repetition 3 times, enzyme activity size represents with the form of Rate activity, and unit is U/mg albumen.Result represents with mean+SD.Data use SPSS13.0 to carry out one factor analysis of variance, and diversity is significantly defined as
p<0.05, represents with " * ".
Embodiment 2:
Get Herba Taraxaci 30g, Radix Sophorae Flavescentis 20g, after adding 2500g water boil half an hour, abandon medicinal residues, zinc gluconate 5g, manganese gluconate 45g are dissolved in decoction liquor, be prepared into immunostimulant and use liquid.
The immunostimulant of above-mentioned preparation is splashed in screw clamp breeding water body 2. with the amount of 0.1g/L, supports 20 days temporarily subsequently.4. tank is used as reference examples, does not add immunostimulant.Change the operations such as water, sampling and immune Enzyme activity assay with embodiment 1.
Embodiment 3:
Get Herba Taraxaci 25g, Radix Sophorae Flavescentis 27g, after adding 2600g water boil half an hour, abandon medicinal residues, zinc gluconate 2g, manganese gluconate 46g are dissolved in decoction liquor, be prepared into immunostimulant and use liquid.
The immunostimulant of above-mentioned preparation is splashed in screw clamp breeding water body 3. with the amount of 0.1g/L, supports 20 days temporarily subsequently.4. tank is used as reference examples, does not add immunostimulant.Change the operations such as water, sampling and immune Enzyme activity assay with embodiment 1.
Interpretation of result:
Embodiment 1,2,3 on the impact of Apostichopus japonicus immunity relevant enzyme vigor as shown in Fig. 1 ~ 5: 5 days, 10 days, 20 days after using immunostimulant, in Apostichopus japonicus coelomic fluid, the vigor of acid phosphatase, alkali phosphatase, superoxide dismutase, lysozyme and phenol oxidase is all significantly higher than reference examples, wherein, the vigor of acid phosphatase and phenol oxidase is promoted to more than 2 times of reference examples, and embodiment 3 is totally better than embodiment 1 and embodiment 2 to the activation effect of immune relevant enzyme.In selected immune indexes, acid phosphatase and alkali phosphatase directly kill by hydrolysis or suppress pathogen, and energy enhancing body is to the phagocytosis of cause of disease; Superoxide dismutase can remove the excess activity oxygen produced in the processes such as immune response, and protection body, simultaneously can also catalysis superoxide anion from the injury of excess activity oxygen, produces the H with very high bactericidal activity
2o
2, participate in sterilization, antibacterial process directly; Lysozyme can destroy the cell wall of antibacterial, thus kills the pathogen of invasion; Phenol oxidase can be killed and wounded by enzymatic reaction, suppress and coagulation cause of disease, and promote encapsulation and engulf, injury repairing, the panimmunity activity such as tuberosity formation.In embodiment 1,2,3 pairs of Apostichopus japonicus bodies, the remarkable activation effect of immune relevant enzyme shows, composite immune reinforcing agent of the present invention significantly can improve the immunity of Apostichopus japonicus, and effect is quick, lasting, stable.
Those of ordinary skill in the art can understand, and in protection scope of the present invention, modifies for above-described embodiment, and it is all possible for adding and replacing, and it does not all exceed protection scope of the present invention.
Claims (3)
1. an Apostichopus japonicus composite immune reinforcing agent, is characterized in that comprising following weight percent composition:
Zinc gluconate 1 ~ 5%;
Manganese gluconate 45 ~ 49%;
Herba Taraxaci 20 ~ 30%;
Radix Sophorae Flavescentis 20 ~ 30%.
2. Apostichopus japonicus composite immune reinforcing agent according to claim 1, is characterized in that each component and percentage by weight are:
Zinc gluconate 2%;
Manganese gluconate 46%;
Herba Taraxaci 25%;
Radix Sophorae Flavescentis 27%.
3. the preparation method of Apostichopus japonicus composite immune reinforcing agent according to claim 1, it is characterized in that comprising the following steps: after Herba Taraxaci and Radix Sophorae Flavescentis two kinds of Chinese medicine ingredients are added 50 times of soak by water, discard medicinal residues, again zinc gluconate and manganese gluconate two kinds of mineralogical compositions are dissolved in decocting for Chinese herbal medicine liquid, then splash in breeding water body according to the dose of 0.1g/L.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107582662A (en) * | 2017-08-29 | 2018-01-16 | 大连海洋大学 | The quick Chinese herbal formula for repairing apostichopus japonicus body-wall skin tissue |
| CN113875909A (en) * | 2021-10-12 | 2022-01-04 | 辽宁省海洋水产科学研究院 | Preparation method of synergistic composite immunopotentiator for stichopus japonicus |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103156093A (en) * | 2013-03-29 | 2013-06-19 | 厦门大学 | Compound vitamin preparation for cultivating stichopus japonicus in summer |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103156093A (en) * | 2013-03-29 | 2013-06-19 | 厦门大学 | Compound vitamin preparation for cultivating stichopus japonicus in summer |
Non-Patent Citations (1)
| Title |
|---|
| 肖克宇主编: "《水产动物免疫学》", 30 June 2011, 中国农业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107582662A (en) * | 2017-08-29 | 2018-01-16 | 大连海洋大学 | The quick Chinese herbal formula for repairing apostichopus japonicus body-wall skin tissue |
| CN113875909A (en) * | 2021-10-12 | 2022-01-04 | 辽宁省海洋水产科学研究院 | Preparation method of synergistic composite immunopotentiator for stichopus japonicus |
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