CN105360716A - Feed additive adjusting trionyx sinensis body color - Google Patents

Feed additive adjusting trionyx sinensis body color Download PDF

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Publication number
CN105360716A
CN105360716A CN201510757649.2A CN201510757649A CN105360716A CN 105360716 A CN105360716 A CN 105360716A CN 201510757649 A CN201510757649 A CN 201510757649A CN 105360716 A CN105360716 A CN 105360716A
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parts
chinese medicine
trionyx sinensis
composition
shelled turtle
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CN201510757649.2A
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Chinese (zh)
Inventor
童明艳
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Hangzhou Yier Biotechnology Co Ltd
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Hangzhou Yier Biotechnology Co Ltd
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Priority to CN201510757649.2A priority Critical patent/CN105360716A/en
Publication of CN105360716A publication Critical patent/CN105360716A/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

Abstract

The invention relates to a feed additive adjusting trionyx sinensis body color. The feed additive is characterized in that the feed additive is composed of a traditional Chinese medicine adjusting composition and a microbe adjusting composition, the traditional Chinese medicine adjusting composition is composed of tagetes erecta L., indigowoad leaf, radix astragali, root of pilose asiabell and fructus crataegi, and the microbe adjusting composition is composed of bacillus subtilis, bifidobacteria and lactics. The feed additive is reasonable in compatibility, and abundant and balanced in nutrition, is not added with synthetic additives harmful to trionyx sinensis and human body, possesses anti-inflammatory anti-bacterial and bacteriostatic efficacies, enhances shrimp immunity, is capable of guaranteeing health growth of trionyx sinensis during culture, also is added with traditional Chinese medicinal materials containing natural pigments, and is capable of improving the body color of trionyx sinensis.

Description

A kind of feed addictive regulating Shelled Turtle Trionyx Sinensis body colour
Technical field
The present invention relates to feed additive field, particularly a kind of feed addictive regulating Shelled Turtle Trionyx Sinensis body colour.
Background technology
Shelled Turtle Trionyx Sinensis, has another name called water fish, soft-shelled turtle, the soft-shelled turtle, is common cultivation tortoise kind, is a kind of oviparity amphibians faunal, its head portrait tortoise, but carapace does not have the striped as tortoise, and edge is soft shape shirt rim, and color is blackish green; Soft-shelled turtle is everlasting the freshwater life of water-bed silt bottom, has the habit of debarking and taking a solar bath; Carnivorous, with fish, shrimp, mollusk etc. for staple food, many prowl at nights, melon skin fruit bits, green grass and cereal etc. are also eaten; Turtle delicious, every hectogram meat is containing 16.5 grams, protein, fat and calcium, phosphorus, iron etc., and nutritive value is very high, and being nourishing treasure, is the aquatic products that a kind of people like very much.Wild Shelled Turtle Trionyx Sinensis is all visible in China, Japan, North Vietnam, Korea S, Russian east.
Shelled Turtle Trionyx Sinensis mostly is to be propagated artificially, along with increase, the culture-cycle shortening of cultivation popularization, cultivation density, after Shelled Turtle Trionyx Sinensis eats artifical compound feed for a long time, body colour often bleaches, be that the Shelled Turtle Trionyx Sinensis difference of yellow cultivars is very large with body colour, its economic worth also can decline to a great extent because of the change of body colour.
China Patent Publication No. CN101411395A, patent publication date on April 22nd, 2009, disclose a kind of feed addictive that can improve body color of aquatic animal, this kind is by astaxanthin, Dunaliella salina, vitamin E, radix bupleuri, canthaxanthin, antioxidant and corn protein powder, this kind of feed addictive can improve body colour that is yellow and red colour system aquatic products, make the body colour of aquatic products present natural yellow or redness, and effectively can improve the immunity of aquatic class, fertility and meat quality.But the raw material of this kind of feeding additive aquatic animal many employings chemical industry synthesis, can exist unavoidably and bad impact is caused on aquatic products quality, and in emphasis food security and quality instantly, use chemical industry synthesis material, as food additives, can affect the desire to purchase of consumer unavoidably.And mostly each component contained in this additive is to improve the artificial of aquatic products body colour or natural colouring matter, and lack the additive that can promote aquatic products appetite or food-intake, improve the inefficiency of aquatic products body colour, effect is poor.
Summary of the invention
The present invention in order to overcome existing adjustment Shelled Turtle Trionyx Sinensis body colour feed addictive in above shortcomings, a kind of feed addictive regulating Shelled Turtle Trionyx Sinensis body colour is provided, this kind of compound additive can improve the body colour of Shelled Turtle Trionyx Sinensis, improve its immunity simultaneously, strengthen the vigor of Shelled Turtle Trionyx Sinensis, and then improve survival rate and the speed of growth of Shelled Turtle Trionyx Sinensis.
Regulate a feed addictive for Shelled Turtle Trionyx Sinensis body colour, be made up of the component of following weight portion: Chinese medicine regulates composition 41 ~ 69 parts, microorganism regulates composition 50 ~ 90 parts.
Described Chinese medicine regulates composition to be obtained by the raw material of following weight portion: marigold 30 ~ 50 parts, folium isatidis 4 ~ 6 parts, the Radix Astragali 2 ~ 4 parts, Radix Codonopsis 2 ~ 4 parts, hawthorn 3 ~ 5 parts.Marigold, folium isatidis and Radix Codonopsis all contain abundant nutrition, also have effect of anti-inflammation, can reduce the bacteriological infection of Shelled Turtle Trionyx Sinensis in breeding process, Radix Codonopsis also has effect of anti-gastric mucosa injure simultaneously, can promote that Shelled Turtle Trionyx Sinensis stomach moves, promote the growth of Shelled Turtle Trionyx Sinensis.The Radix Astragali is except containing except the saponin(e that can play antibacterial action, also containing flavone compound, immunologic function can be strengthened except having, strengthening body hypoxia-bearing and stress ability, except promotion organism metabolism, antibacterial and antiviral effect, can also play a role in improving to the body colour of Shelled Turtle Trionyx Sinensis.
Described microorganism regulates composition to be made up of the component of following weight portion: bacillus subtilis 30 ~ 50 parts, Bifidobacterium 10 ~ 20 parts, lactic acid bacteria 10 ~ 20 parts.Bacillus subtilis can produce AMS and neutral proteinase, and starchiness nutriment contained in feed can be decomposed into micromolecular polysaccharide by AMS, is more conducive to the absorption of Shelled Turtle Trionyx Sinensis; Protide nutriment in feed can be decomposed into the micromolecule polypeptide more easily absorbed by neutral proteinase under mild conditions, is more conducive to the growth promoting Shelled Turtle Trionyx Sinensis, thus can improves the body colour of Shelled Turtle Trionyx Sinensis; Bacillus subtilis can also produce the various active such as subtilin, polymyxins material, to pathogenic bacteria and autogenous infection inhibited, the diseases such as Shelled Turtle Trionyx Sinensis gill rot can be prevented.Bifidobacterium can promote that Shelled Turtle Trionyx Sinensis is to the absorption of feed Middle nutrition material.Lactic acid bacteria has the function promoting the absorption of nutrient ingredients such as protein, can promote that Shelled Turtle Trionyx Sinensis absorbs nutrition; There is immunoregulatory function, immunity and the resistance of Shelled Turtle Trionyx Sinensis can be strengthened; Can secrete B family vitamin and Shelled Turtle Trionyx Sinensis grows necessary amino acid, be the same more abundant balanced in feed; Lactic acid bacteria can suppress harmful bacteria growth in feed in addition, improves the effect of feed quality.
As preferably, Chinese medicine regulates composition to be obtained by following steps:
(1) regulated by Chinese medicine each raw material of composition to be mixed into Traditional Chinese drug mixture, and by Traditional Chinese drug mixture and mass concentration be 85 ~ 95% ethanol mix obtained Chinese medicine according to the solid-liquid ratio of 1g:8 ~ 10mL and regulate composition semifinished product;
(2) Chinese medicine of gained is regulated composition semifinished product heating and refluxing extraction 1 ~ 3 hour under 80 ~ 85 DEG C of conditions, after filtration, filtrate reduced in volume process is obtained Chinese medicine instrumentality concentrate;
(3) Chinese medicine instrumentality concentrate vacuum drying treatment at 50 ~ 60 DEG C that will be obtained by step (2), pulverizes after drying again, and crosses 80 ~ 100 mesh sieves, and obtained Chinese medicine regulates composition.
As preferably, Chinese medicine regulates composition to be obtained by the raw material of following weight portion: marigold 35 parts, folium isatidis 4.4 parts, the Radix Astragali 2.5 parts, Radix Codonopsis 2.8 parts, hawthorn 4 parts.
As preferably, microorganism regulates composition to be made up of the component of following weight portion: bacillus subtilis 37 parts, Bifidobacterium 16 parts, lactic acid bacteria 13 parts.
Therefore, the present invention has following beneficial effect:
(1) compatibility is reasonable, nutritious and balanced, does not add Shelled Turtle Trionyx Sinensis and the harmful synthetic additive of human body, green health;
(2) add the Chinese medicine with anti-inflammatory, antibacterial and bacteriostasis efficacy, strengthen its immunity, Shelled Turtle Trionyx Sinensis healthy growth in breeding process can be ensured, improve the body colour of Shelled Turtle Trionyx Sinensis;
(3) add the microorganism inoculant that multiple nutrients material can be provided, the nutriment in feed can be decomposed into the Small molecular more easily absorbed, be beneficial to the absorption of Shelled Turtle Trionyx Sinensis, promote the growth of Shelled Turtle Trionyx Sinensis, improve the body colour of Shelled Turtle Trionyx Sinensis;
(4) add the Chinese medicine containing natural colouring matter, the body colour of Shelled Turtle Trionyx Sinensis can be improved.
Detailed description of the invention
Below by specific embodiment, technical scheme of the present invention is described in further detail.
Embodiment 1
Regulate a feed addictive for Shelled Turtle Trionyx Sinensis body colour, be made up of the component of following weight portion: Chinese medicine regulates composition 41 parts, microorganism regulates composition 50 parts;
Described Chinese medicine regulates composition to be obtained by the raw material of following weight portion: marigold 30 parts, folium isatidis 4 parts, the Radix Astragali 2 parts, Radix Codonopsis 2 parts, hawthorn 3 parts;
Described microorganism regulates composition to be made up of the component of following weight portion: bacillus subtilis 30 parts, Bifidobacterium 10 parts, lactic acid bacteria 10 parts.
Described Chinese medicine regulates composition to be obtained by following steps:
(1) regulated by Chinese medicine each raw material of composition to be mixed into Traditional Chinese drug mixture, and by Traditional Chinese drug mixture and mass concentration be 85% ethanol mix obtained Chinese medicine according to the solid-liquid ratio of 1g:8mL and regulate composition semifinished product;
(2) Chinese medicine of gained is regulated composition semifinished product heating and refluxing extraction 1 hour under 80 DEG C of conditions, after filtration, filtrate reduced in volume process is obtained Chinese medicine instrumentality concentrate;
(3) Chinese medicine instrumentality concentrate vacuum drying treatment at 50 DEG C that will be obtained by step (2), pulverizes after drying again, and crosses 80 mesh sieves, and obtained Chinese medicine regulates composition.
Embodiment 2
Regulate a feed addictive for Shelled Turtle Trionyx Sinensis body colour, be made up of the component of following weight portion: Chinese medicine regulates composition 48.7 parts, microorganism regulates composition 66 parts;
Described Chinese medicine regulates composition to be obtained by the raw material of following weight portion: marigold 35 parts, folium isatidis 4.4 parts, the Radix Astragali 2.5 parts, Radix Codonopsis 2.8 parts, hawthorn 4 parts;
Described microorganism regulates composition to be made up of the component of following weight portion: bacillus subtilis 37 parts, Bifidobacterium 16 parts, lactic acid bacteria 13 parts.
Described Chinese medicine regulates composition to be obtained by following steps:
(1) regulated by Chinese medicine each raw material of composition to be mixed into Traditional Chinese drug mixture, and by Traditional Chinese drug mixture and mass concentration be 90% ethanol mix obtained Chinese medicine according to the solid-liquid ratio of 1g:9mL and regulate composition semifinished product;
(2) Chinese medicine of gained is regulated composition semifinished product heating and refluxing extraction 2 hours under 83 DEG C of conditions, after filtration, filtrate reduced in volume process is obtained Chinese medicine instrumentality concentrate;
(3) Chinese medicine instrumentality concentrate vacuum drying treatment at 55 DEG C that will be obtained by step (2), pulverizes after drying again, and crosses 90 mesh sieves, and obtained Chinese medicine regulates composition.
Embodiment 3
Regulate a feed addictive for Shelled Turtle Trionyx Sinensis body colour, be made up of the component of following weight portion: Chinese medicine regulates composition 69 parts, microorganism regulates composition 90 parts;
Described Chinese medicine regulates composition to be obtained by the raw material of following weight portion: marigold 50 parts, folium isatidis 6 parts, the Radix Astragali 4 parts, Radix Codonopsis 4 parts, hawthorn 5 parts;
Described microorganism regulates composition to be made up of the component of following weight portion: bacillus subtilis 50 parts, Bifidobacterium 20 parts, lactic acid bacteria 20 parts.
Described Chinese medicine regulates composition to be obtained by following steps:
(1) regulated by Chinese medicine each raw material of composition to be mixed into Traditional Chinese drug mixture, and by Traditional Chinese drug mixture and mass concentration be 95% ethanol mix obtained Chinese medicine according to the solid-liquid ratio of 1g:10mL and regulate composition semifinished product;
(2) Chinese medicine of gained is regulated composition semifinished product heating and refluxing extraction 3 hours under 85 DEG C of conditions, after filtration, filtrate reduced in volume process is obtained Chinese medicine instrumentality concentrate;
(3) Chinese medicine instrumentality concentrate vacuum drying treatment at 60 DEG C that will be obtained by step (2), pulverizes after drying again, and crosses 100 mesh sieves, and obtained Chinese medicine regulates composition.
When proportioning feed, a kind of feed addictive of Shelled Turtle Trionyx Sinensis body colour that regulates of the present invention accounts for 9 ~ 16% of feed gross mass.
Effect test:
Feeding experiment is carried out in greenhouse, and young soft-shelled turtle basically identical for commercially available body weight is divided into 2 groups at random: test group is test I group and test II group, and often group is 1000, supports respectively and is 50m at area 2pond in, test I group and throw something and feed and be added with the present invention and regulate the puffing granule compound feed of the feed addictive of Shelled Turtle Trionyx Sinensis body colour (feed addictive addition is 13% of feed gross weight), test II group of basestocks puffing granule compound feed of throwing something and feeding.Control between feeding period that water temperature is 30 ± 1 DEG C, temperature is 32 ± 2 DEG C, the depth of water is grown up to be added gradually by 30cm with soft-shelled turtle individuality and is deep to 80cm, full water quality is regulated by regularly splash quick lime and EM microbial inoculum of pond, and refreshes the water periodically 20%, to ensure that water quality is good.Experimental period is 200 days, throws something and feeds every day three times.Total carotinoid and lutein in period sampling measuring weightening finish, survival rate, skin.
Shelled Turtle Trionyx Sinensis quantity and weight is added up by group, the situations such as duration of test observed and recorded soft-shelled turtle ingests, activity, growth before and after test.Two groups of soft-shelled turtles, between greenhouse feeding period, are tested I group and obviously strengthen than the appetite of test II group, have a medical check-up heavily at random at every turn, test I group than test II group and control group speed of weight increment fast.When going out pond, soft-shelled turtle gross weight is the highest to test I group, tests II group and takes second place; Individual counterpoise is then that test I group of > tests II group; Morbidity death toll tests II group more than test I group (see table 1).
The weightening finish of table 1 test group and control group Shelled Turtle Trionyx Sinensis and death condition
Group Throw in gross weight/kg Individual Size/g Survival rate/% Go out pond gross weight/kg Go out the individual counterpoise/g in pond Feed coefficient
Test I group 49 23-32 93.8 564.3 473 1.04
Test II group 50 26-33 77.6 321.7 396 1.18
Experimental result shows, test the survival rate of I group, increase weight apparently higher than test II group, feed coefficient also obviously reduces.Produce food calling effect, resisting pathogenic microbes effect that these effects may regulate composition tool stronger with the Chinese medicine in feed and regulate body immunization to have direct relation.Aquatic livestock self can not synthesize carotenoid and lutein, can only obtain from feed, food, if do not add colouring agent in the mixed feed eaten of the fish propagated artificially or content is very low, is just not enough to meet its needs, thus body colour is shoaled.
The content (" the pond culture Shelled Turtle Trionyx Sinensis skin pigment content analysis " of assay method reference Zhang Bingjie, leaf unit soil, contemporary aquatic products, 2013.11) of the carotenoid in mensuration Shelled Turtle Trionyx Sinensis skin and lutein.
The mensuration of total carotinoid total content, the results are shown in Table 2:
Carotenoid content mg/kg in table 2 Shelled Turtle Trionyx Sinensis skin
Group Test I group Test II group
Carotenoid content mg/kg 421.3 368.6
The mensuration of lutein content, the results are shown in Table 3:
Table 3 Shelled Turtle Trionyx Sinensis skin Lutein cellulose content, mg/kg
Group Test I group Test II group
Lutein content mg/kg 1.89 1.49
Shown by table 2 and table 3, test the content of carotenoid in I group of Shelled Turtle Trionyx Sinensis skin and lutein apparently higher than test II group, visible Chinese medicine regulates composition and microorganism to regulate composition to facilitate the utilization of Shelled Turtle Trionyx Sinensis to lutein.
Above-described embodiment is one of the present invention preferably scheme, not does any pro forma restriction to the present invention, also has other variant and remodeling under the prerequisite not exceeding the technical scheme described in claim.

Claims (4)

1. regulate a feed addictive for Shelled Turtle Trionyx Sinensis body colour, it is characterized in that it is made up of the component of following weight portion: Chinese medicine regulates composition 41 ~ 69 parts, microorganism regulates composition 50 ~ 90 parts;
Described Chinese medicine regulates composition to be obtained by the raw material of following weight portion: marigold 30 ~ 50 parts, folium isatidis 4 ~ 6 parts, the Radix Astragali 2 ~ 4 parts, Radix Codonopsis 2 ~ 4 parts, hawthorn 3 ~ 5 parts;
Described microorganism regulates composition to be made up of the component of following weight portion: bacillus subtilis 30 ~ 50 parts, Bifidobacterium 10 ~ 20 parts, lactic acid bacteria 10 ~ 20 parts.
2. a kind of feed addictive regulating Shelled Turtle Trionyx Sinensis body colour according to claim 1, is characterized in that: described Chinese medicine regulates composition to be obtained by following steps:
(1) regulated by Chinese medicine each raw material of composition to be mixed into Traditional Chinese drug mixture, and by Traditional Chinese drug mixture and mass concentration be 85 ~ 95% ethanol mix obtained Chinese medicine according to the solid-liquid ratio of 1g:8 ~ 10mL and regulate composition semifinished product;
(2) Chinese medicine of gained is regulated composition semifinished product heating and refluxing extraction 1 ~ 3 hour under 80 ~ 85 DEG C of conditions, after filtration, filtrate reduced in volume process is obtained Chinese medicine instrumentality concentrate;
(3) Chinese medicine instrumentality concentrate vacuum drying treatment at 50 ~ 60 DEG C that will be obtained by step (2), pulverizes after drying again, and crosses 80 ~ 100 mesh sieves, and obtained Chinese medicine regulates composition.
3. a kind of feed addictive regulating Shelled Turtle Trionyx Sinensis body colour according to claim 1, it is characterized in that: described Chinese medicine regulates composition to be obtained by the raw material of following weight portion: marigold 35 parts, folium isatidis 4.4 parts, the Radix Astragali 2.5 parts, Radix Codonopsis 2.8 parts, hawthorn 4 parts.
4. a kind of feed addictive regulating Shelled Turtle Trionyx Sinensis body colour according to claim 1, is characterized in that: described microorganism regulates composition to be made up of the component of following weight portion: bacillus subtilis 37 parts, Bifidobacterium 16 parts, lactic acid bacteria 13 parts.
CN201510757649.2A 2015-11-09 2015-11-09 Feed additive adjusting trionyx sinensis body color Pending CN105360716A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107223776A (en) * 2017-07-07 2017-10-03 芜湖市木子生态园有限公司 A kind of green and healthy trionyx sinensis feed additive
CN108094766A (en) * 2018-01-24 2018-06-01 东海县水产技术推广站 The carp fooder additive and its application of a kind of Radix Astragali and compound probiotic compatibility
CN109156624A (en) * 2018-09-28 2019-01-08 河南科技大学 A kind of immunopotentiator and the preparation method and application thereof improving soft-shelled turtle premunition

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107223776A (en) * 2017-07-07 2017-10-03 芜湖市木子生态园有限公司 A kind of green and healthy trionyx sinensis feed additive
CN108094766A (en) * 2018-01-24 2018-06-01 东海县水产技术推广站 The carp fooder additive and its application of a kind of Radix Astragali and compound probiotic compatibility
CN109156624A (en) * 2018-09-28 2019-01-08 河南科技大学 A kind of immunopotentiator and the preparation method and application thereof improving soft-shelled turtle premunition

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Application publication date: 20160302