CN109156624A - A kind of immunopotentiator and the preparation method and application thereof improving soft-shelled turtle premunition - Google Patents

A kind of immunopotentiator and the preparation method and application thereof improving soft-shelled turtle premunition Download PDF

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Publication number
CN109156624A
CN109156624A CN201811141330.7A CN201811141330A CN109156624A CN 109156624 A CN109156624 A CN 109156624A CN 201811141330 A CN201811141330 A CN 201811141330A CN 109156624 A CN109156624 A CN 109156624A
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China
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powder
shelled turtle
parts
immunopotentiator
soft
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Inventor
孙平
朱文琰
曹慧
张彦坤
王娅琳
张纪亮
徐世晓
高小蝉
张春暖
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Henan University of Science and Technology
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Henan University of Science and Technology
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins

Abstract

The invention belongs to feed additive fields, and in particular to a kind of immunopotentiator and the preparation method and application thereof for improving soft-shelled turtle premunition.The immunopotentiator of the raising soft-shelled turtle premunition, it is prepared by immunopotentiating composition and zymophyte powder fermentation, wherein, immunopotentiating composition includes following component: Cortex Phellodendri, rheum officinale, Divine Comedy, dried orange peel, hawthorn, Radix Glycyrrhizae, Prunella vulgaris, Herba Cistanches, Radix Astragali, Radix Codonopsis, pimiento, radix bupleuri, medical stone and sulphur;Zymophyte powder includes lactobacillus acidophilus powder, bacillus subtilis powder, saccharomycete powder and photosynthetic bacteria powder.The immunopotentiator everfermentation provided by the invention for improving soft-shelled turtle premunition, macromolecular substances are further broken into the small-molecule substance for being easy to absorb, the digestion and absorption function and enhancing soft-shelled turtle immunity function and resistant to diseases and insects to nutriment can be enhanced, have the characteristics that it is pure natural, have no toxic side effect, be pollution-free, without obvious residual, have nutrition and medicinal effect concurrently.

Description

A kind of immunopotentiator and the preparation method and application thereof improving soft-shelled turtle premunition
Technical field
The invention belongs to feed additive fields, and in particular to a kind of immunopotentiator and its system for improving soft-shelled turtle premunition Preparation Method and application.
Background technique
Soft-shelled turtle is a kind of oviparity amphibians faunal, and meat is full of nutrition, contains collagen, keratin, copper, vitamin The nutrients such as D have the laudatory title of " cuisines five tastes meat ";With enhancing disease resistance, adjusts endocrine and improve the function such as breast milk quality Effect.Soft-shelled turtle bone is rare Chinese medicine, being capable of nourishing yin and lessening fire.A kind of very high economic animal of culture efficiency is become, but it is given birth to The clean fearness of habit happiness living is dirty, like positive fear of wind, happiness is quiet is afraid of to shy, more demanding to cultivating condition.Therefore, in order to reduce soft-shelled turtle sick rate, It increases economic efficiency, it is anti-that aquaculture generallys use the enhancing animal such as chemical antimicrobial, immunopotentiator, antibiotic and microelement Sick power, but also result in the drawbacks such as pollution environment, medicament residue and aggravation drug resistance.With going deep into for development of Chinese herb products, Chinese medicine is with it It is pure natural, resource is wide, toxicity is low, noresidue and the advantages such as have no toxic side effect, be increasingly subject to cultivation industry favor.Contain in Chinese medicine The organic principles such as plant polyose, flavones, albumen, alkaloid, saponin, tannic acid matter in addition to nutriment is provided, also have anti- Bacterium is antiviral, anti-inflammatory, calmness, it is anticonvulsion, improve oxidation resistance, adjust the effects of immunity;And it is dynamic to improve cultivation The qualities such as object meat, egg, milk.The research and development that biofermentation technique in recent years is applied to Traditional Chinese drug microecology feed addictive make macromolecular Nutriment resolves into the small molecule for being easy to absorb, and effectively improves utilization rate;And enhance animal disease resistant power, aquaculture cost is reduced, Improve culture efficiency.
Summary of the invention
For overcome the deficiencies in the prior art and disadvantage, the primary purpose of the present invention is that it is disease-resistant to provide a kind of raising soft-shelled turtle The immunopotentiator of power, the immunopotentiator can reduce breeding environment oxidative stress caused by soft-shelled turtle, improve the anti-of soft-shelled turtle and answer Sharp and disease resistance, improves the digestion and absorption efficiency of nutriment, promotes the growth and development of soft-shelled turtle, improves production performance.
Another object of the present invention is to provide the preparation methods of the immunopotentiator of above-mentioned raising soft-shelled turtle premunition.
A further object of the present invention is to provide the applications of the immunopotentiator of above-mentioned raising soft-shelled turtle premunition.
The purpose of the invention is achieved by the following technical solution:
A kind of immunopotentiator improving soft-shelled turtle premunition is prepared by immunopotentiating composition and zymophyte powder fermentation It arrives;
The immunopotentiating composition includes component as follows in parts by mass:
6~10 parts of Cortex Phellodendri, 4~6 parts of rheum officinale, 5~10 parts of Divine Comedy, 4~7 parts of dried orange peel, 3~8 parts of hawthorn, 6~7 parts of Radix Glycyrrhizae, 7~9 parts of Prunella vulgaris, 6~8 parts of Herba Cistanches, 5~7 parts of Radix Astragali, 5~7 parts of Radix Codonopsis, 5~8 parts of pimiento, 6~8 parts of radix bupleuri, wheat meal 6~8 parts of stone, 4~6 parts of sulphur;
The zymophyte powder includes lactobacillus acidophilus powder, bacillus subtilis powder, saccharomycete powder and photosynthetic bacteria powder;
The lactobacillus acidophilus powder, bacillus subtilis powder, saccharomycete powder and photosynthetic bacteria powder mass ratio be preferably 3:(2~4): 2:(1~2);
Lactobacillus acidophilus powder, bacillus subtilis powder, saccharomycete powder and the photosynthetic bacteria powder be preferably not less than 1 × 109cfu/g;
The immunopotentiator of the high soft-shelled turtle premunition also includes multi-vitamins, wherein multi-vitamins and Cortex Phellodendri Mass ratio be preferably (8~12): (6~10);
The immunopotentiating composition preferably comprises following component in parts by mass:
8 parts of Cortex Phellodendri, 5 parts of rheum officinale, 9 parts of Divine Comedy, 6 parts of dried orange peel, 5 parts of hawthorn, 6 parts of Radix Glycyrrhizae, 8 parts of Prunella vulgaris, 7 parts of Herba Cistanches, 6 parts of Radix Astragali, 5 parts of Radix Codonopsis, 6 parts of pimiento, 7 parts of radix bupleuri, 5 parts of sulphur, 7 parts of medical stone, 10 parts of multi-vitamins;
The preparation method of the immunopotentiator of the raising soft-shelled turtle premunition, comprises the following steps:
(1) by the component Cortex Phellodendri of immunopotentiating composition, rheum officinale, Divine Comedy, dried orange peel, hawthorn, Radix Glycyrrhizae, Prunella vulgaris, Herba Cistanches, Radix Astragali, Radix Codonopsis, pimiento and radix bupleuri pulverize, and sieving obtains mixing fine powders;
(2) the component medical stone and sulphur of immunopotentiating composition are smashed, levigation respectively, is sieved, it is thin obtains medical stone Powder and sulphur fine powder;
(3) medical stone fine powder made from mixing fine powders made from (1) step, step (2) and sulphur fine powder are mixed, is obtained Immunopotentiating composition;
(4) lactobacillus acidophilus powder, bacillus subtilis powder, saccharomycete powder and photosynthetic bacteria powder are mixed, obtains zymophyte Zymophyte powder is inoculated in LB culture medium by powder according to the ratio of 6~10wt%, and 36~38 DEG C of 40~50h of culture obtain level-one Seed liquor;Primary seed solution is inoculated in LB culture medium according to the ratio of 6~10wt%, 36~38 DEG C of 35~45h of culture are obtained To secondary seed solution;
(5) by secondary seed solution made from step (4) according to mass ratio 1:(1~1.5) be added step (3) made from be immunized Enhance in composition, 37~39 DEG C of 36~72h of fermentation dry, pulverize, be improved the immunopotentiator of soft-shelled turtle premunition;Such as The component that fruit improves the immunopotentiator of soft-shelled turtle premunition also includes multi-vitamins, then by multi-vitamins and smashed production Object is mixed with multi-vitamins, is improved the immunopotentiator of soft-shelled turtle premunition;
Crushing described in step (1) preferably pulverizes;
Sieving described in step (1) was preferably 30~40 meshes;
Sieving described in step (2) was preferably 30~50 meshes;
The inoculum concentration of zymophyte powder described in step (4) is preferably 8wt%;
Cultivation temperature described in step (4) is preferably 37 DEG C;
LB culture medium described in step (4) includes following component: tryptone (Tryptone) 9g/L, yeast extract (Yeast extract) 7g/L and sodium chloride (NaCl) 7g/L, pH7.4;
Preferred 30~the 50min of ultraviolet sterilization of immunopotentiating composition described in step (5);
The mass ratio of secondary seed solution described in step (5) and immunopotentiating composition is preferably 1:1.1;
Fermentation condition described in step (5) is preferably 38 DEG C of fermentation 48h;
Drying described in step (5) is preferably low temperature drying;
The immunopotentiator of the raising soft-shelled turtle premunition can be mixed further with enzyme process maltodextrin, be prepared and mentioned The immunopotentiator particle of high soft-shelled turtle premunition;
The immunopotentiator of raising soft-shelled turtle premunition and the mass ratio of enzyme process maltodextrin is preferably 4:1;
The partial size of the immunopotentiator particle of the raising soft-shelled turtle premunition is preferably 15~20 mesh;
Application of the immunopotentiator of the raising soft-shelled turtle premunition in soft-shelled turtle raising field;
When raising soft-shelled turtle, young soft-shelled turtle starts to add, and at during the soft-shelled turtle phase, improves the dosage of the immunopotentiator of soft-shelled turtle premunition It is the 1~2% of soft-shelled turtle feed total weight;At the soft-shelled turtle phase and after, improve soft-shelled turtle premunition immunopotentiator dosage be soft-shelled turtle The 3~5% of feed total weight;
The principle of the present invention:
It is provided by the invention improve soft-shelled turtle premunition immunopotentiator in, Cortex Phellodendri have strong nourishing, stomach strengthening and digestion promoting, clearly Cool convergence effect;There is rheum officinale clearing heat and removing internal heat, cool blood to conduct oneself in society, detoxification and removing stasis and other effects;Divine Comedy, dried orange peel, hawthorn three appetizing are strong Spleen improves appetite, enhancing digestive function;It invigorates the spleen and benefits qi, it is clearing heat and detoxicating, alleviate drug toxicity, strong.Radix Glycyrrhizae has tonifying spleen benefit Gas, it is clearing heat and detoxicating, alleviate dealed with medicine went;Rhizome of cyrtomium has effects that desinsection, heat-clearing, removing toxic substances, cooling blood and hemostasis.Prunella vulgaris heat-clearing is rushed down Fire, mass dissipating and swelling eliminating have antibacterial action to comma bacillus, typhoid bacillus, shigella dysenteriae and Escherichia coli etc..Herba Cistanches, which have, to be adjusted Whole endocrine promotes metabolism, strong body and strengthen immunity effect.Radix Astragali and Radix Codonopsis tonifying middle-Jiao and Qi, enrich blood, build up health, Anti-aging.Pimiento is rich in vitamin A, B, C, dietary fiber and minerals etc., acrid flavour heat, spleen benefiting and stimulating the appetite.Radix bupleuri and inducing diaphoresis In, soothing liver-qi stagnation, anti-bacteria and anti-virus, enhancing are immune.Sulphur has sterilization, mite killing and insecticidal action.Medical stone, which has to improve, raises Expect utilization rate, promotes the metabolism of organism, anti anoxia, antifatigue, raising animal body immunocompetence.
The present invention further ferments immunopotentiating composition with zymophyte powder, big in one side immunopotentiating composition Molecular substance can be further broken into small-molecule substance, and on the other hand, under the action of zymophyte powder, release more drug is living Property ingredient, and be conducive to zymophyte beneficial metabolic product accumulation.
The present invention has the following advantages and effects with respect to the prior art:
(1) the immunopotentiator everfermentation provided by the invention for improving soft-shelled turtle premunition, on the one hand can by macromolecular substances To be further broken into the small-molecule substance for being easy to absorb, the digestion and absorption function to nutriment can be enhanced, on the other hand existed Under the action of zymophyte powder, more drug active constituent is discharged, and be conducive to the accumulation of zymophyte beneficial metabolic product, it can be significant Enhance soft-shelled turtle immunity function and resistant to diseases and insects.
(2) it is provided by the invention improve soft-shelled turtle premunition immunopotentiator have it is pure natural, have no toxic side effect, without dirt The characteristics of dye, nothing obviously remain, has nutrition and medicinal effect concurrently, promotes digestion and absorption of the soft-shelled turtle to nutriment, effectively increasing Strong soft-shelled turtle immunity, improves meat.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
In embodiment, LB culture medium prescription: tryptone (Tryptone 9g/L, yeast extract (Yeast Extract) 7g/L, sodium chloride (NaCl) 7g/L, distilled water 1L, 1mL 1mol/L NaOH solution adjust pH to 7.4, and high pressure is steamed Vapour sterilizing 20min;
Number as described in the examples is mass parts;Lactobacillus lactis powder, bacillus subtilis powder, saccharomycete powder and photosynthetic Bacterium powder be it is commercially available, clump count be greater than 1 × 109cfu/g;
Multi-vitamins are commercially available soft-shelled turtle multi-vitamins;
Embodiment 1
(1) by 8 parts of the component Cortex Phellodendri of immunopotentiating composition, 5 parts of rheum officinale, 9 parts of Divine Comedy, 6 parts of dried orange peel, 5 parts of hawthorn, Radix Glycyrrhizae 6 parts, 8 parts of Prunella vulgaris, 7 parts of Herba Cistanches, 6 parts of Radix Astragali, 7 parts of 5 parts of Radix Codonopsis, 6 parts of pimiento and radix bupleuri ultra micros in micronizer It crushes, crosses 40 meshes, obtain mixing fine powders, low temperature drying saves, spare;
(2) 7 parts and 5 parts of sulphur of the component medical stone of immunopotentiating composition are smashed, levigation respectively, crosses 50 meshes, obtains To medical stone fine powder and sulphur fine powder, low temperature drying is saved, spare;
(3) medical stone fine powder made from mixing fine powders made from (1) step, step (2) and sulphur fine powder are mixed, in nothing Ultraviolet sterilization 30min in bacterium station, obtains immunopotentiating composition, spare;
(4) by lactobacillus acidophilus powder, bacillus subtilis powder, saccharomycete powder and photosynthetic bacteria powder according to mass ratio 3:3:2: 2 mixing, obtain zymophyte powder, zymophyte powder are inoculated in LB culture medium according to the ratio of 8wt%, 37 DEG C of culture 48h are obtained Primary seed solution;Primary seed solution is inoculated in LB culture medium according to the ratio of 8wt%, 37 DEG C of culture 40h obtain second level kind Sub- liquid;
(5) Immune-enhancing effect made from step (3) is added according to mass ratio 1:1.1 in secondary seed solution made from step (4) In composition, 38 DEG C of fermentation 48h be dry, pulverize, then with the multi-vitamins (matter of Cortex Phellodendri in multi-vitamins and step (1) Amount is than being 10:8) mixing, it is improved the immunopotentiator of soft-shelled turtle premunition;
(6) by the obtained immunopotentiator for improving soft-shelled turtle premunition of step (5) further with enzyme process maltodextrin according to Mass ratio 4:1 mixing, is made the immunopotentiator particle of the raising soft-shelled turtle premunition of 15 mesh, and low temperature drying saves.
Embodiment 2
(1) by 10 parts of the component Cortex Phellodendri of immunopotentiating composition, 5 parts of rheum officinale, 5 parts of Divine Comedy, 6 parts of dried orange peel, 5 parts of hawthorn, sweet 7 parts of grass, 8 parts of Prunella vulgaris, 7 parts of Herba Cistanches, 6 parts of Radix Astragali, 7 parts of Radix Codonopsis, 6 parts of pimiento and 7 parts of radix bupleuri surpass in micronizer Crushing of Ultrafine crosses 30 meshes, obtains mixing fine powders, low temperature drying saves, spare;
(2) 7 parts and 5 parts of sulphur of the component medical stone of immunopotentiating composition are smashed, levigation respectively, crosses 30 meshes, obtains To medical stone fine powder and sulphur fine powder, low temperature drying is saved, spare;
(3) medical stone fine powder made from mixing fine powders made from (1) step, step (2) and sulphur fine powder are mixed, in nothing Ultraviolet sterilization 50min in bacterium station, obtains immunopotentiating composition, spare;
(4) by lactobacillus acidophilus powder, bacillus subtilis powder, saccharomycete powder and photosynthetic bacteria powder according to mass ratio 3:4:2: 1 mixing, obtains zymophyte powder, zymophyte powder is inoculated in LB culture medium according to the ratio of 10wt%, 37 DEG C of culture 40h are obtained To primary seed solution;Primary seed solution is inoculated in LB culture medium according to the ratio of 6wt%, 37 DEG C of culture 48h obtain second level Seed liquor;
(5) Immune-enhancing effect made from step (3) is added according to mass ratio 1:1.5 in secondary seed solution made from step (4) In composition, 38 DEG C of fermentation 48h be dry, pulverize, then with the multi-vitamins (matter of Cortex Phellodendri in multi-vitamins and step (1) Amount is than being 8:10) mixing, it is improved the immunopotentiator of soft-shelled turtle premunition;
(6) by the obtained immunopotentiator for improving soft-shelled turtle premunition of step (5) further with enzyme process maltodextrin according to Mass ratio 4:1 mixing, obtains the immunopotentiator particle of the raising soft-shelled turtle premunition of 20 mesh, and low temperature drying saves.
Embodiment 3
(1) by 6 parts of the component Cortex Phellodendri of immunopotentiating composition, 4 parts of rheum officinale, 10 parts of Divine Comedy, 4 parts of dried orange peel, 3 parts of hawthorn, sweet 6 parts of grass, 7 parts of Prunella vulgaris, 6 parts of Herba Cistanches, 5 parts of Radix Astragali, 7 parts of Radix Codonopsis, 5 parts of pimiento and 6 parts of radix bupleuri surpass in micronizer Crushing of Ultrafine crosses 40 meshes, obtains mixing fine powders, low temperature drying saves, spare;
(2) 8 parts and 4 parts of sulphur of the component medical stone of immunopotentiating composition are smashed, levigation respectively, crosses 30 meshes, obtains To medical stone fine powder and sulphur fine powder, low temperature drying is saved, spare;
(3) medical stone fine powder made from mixing fine powders made from (1) step, step (2) and sulphur fine powder are mixed, in nothing Ultraviolet sterilization 40min in bacterium station, obtains immunopotentiating composition, spare;
(4) by lactobacillus acidophilus powder, bacillus subtilis powder, saccharomycete powder and photosynthetic bacteria powder according to mass ratio 3:3:2: 1.5 mixing, obtain zymophyte powder, zymophyte powder are inoculated in LB culture medium according to the ratio of 6wt%, 36 DEG C of culture 50h are obtained To primary seed solution;Primary seed solution is inoculated in LB culture medium according to the ratio of 10wt%, 36 DEG C of culture 35h obtain two Grade seed liquor;
(5) Immune-enhancing effect made from step (3) is added according to mass ratio 1:1.5 in secondary seed solution made from step (4) In composition, 37 DEG C of fermentation 72h be dry, pulverize, then with the multi-vitamins (matter of Cortex Phellodendri in multi-vitamins and step (1) Amount is than being 12:6) mixing, it is improved the immunopotentiator of soft-shelled turtle premunition;
(6) by the obtained immunopotentiator for improving soft-shelled turtle premunition of step (5) further with enzyme process maltodextrin according to Mass ratio 4:1 mixing, obtains the immunopotentiator particle of the raising soft-shelled turtle premunition of 15 mesh, and low temperature drying saves.
Embodiment 4
(1) by 7 parts of the component Cortex Phellodendri of immunopotentiating composition, 6 parts of rheum officinale, 6 parts of Divine Comedy, 7 parts of dried orange peel, 8 parts of hawthorn, Radix Glycyrrhizae 6 parts, 9 parts of Prunella vulgaris, 8 parts of Herba Cistanches, 7 parts of Radix Astragali, 8 parts of 6 parts of Radix Codonopsis, 8 parts of pimiento and radix bupleuri ultra micros in micronizer It crushes, crosses 50 meshes, obtain mixing fine powders, low temperature drying saves, spare;
(2) 6 parts and 6 parts of sulphur of the component medical stone of immunopotentiating composition are smashed, levigation respectively, crosses 40 meshes, obtains To medical stone fine powder and sulphur fine powder, low temperature drying is saved, spare;
(3) medical stone fine powder made from mixing fine powders made from (1) step, step (2) and sulphur fine powder are mixed, in nothing Ultraviolet sterilization 50min in bacterium station, obtains immunopotentiating composition, spare;
(4) by lactobacillus acidophilus powder, bacillus subtilis powder, saccharomycete powder and photosynthetic bacteria powder according to mass ratio 3:2:2: 2 mixing, obtain zymophyte powder, zymophyte powder are inoculated in LB culture medium according to the ratio of 7wt%, 38 DEG C of culture 45h are obtained Primary seed solution;Primary seed solution is inoculated in LB culture medium according to the ratio of 7wt%, 38 DEG C of culture 45h obtain second level kind Sub- liquid;
(5) Immune-enhancing effect group made from step (3) is added according to mass ratio 1:1 in secondary seed solution made from step (4) Close in object, 39 DEG C of fermentation 36h dry, pulverize, then with the multi-vitamins (quality of Cortex Phellodendri in multi-vitamins and step (1) Than mixing for 9:7), it is improved the immunopotentiator of soft-shelled turtle premunition;
(6) by the obtained immunopotentiator for improving soft-shelled turtle premunition of step (5) further with enzyme process maltodextrin according to Mass ratio 4:1 mixing, obtains the immunopotentiator particle of the raising soft-shelled turtle premunition of 20 mesh, and low temperature drying saves.
Comparative example 1
(1) by 8 parts of Cortex Phellodendri, 5 parts of rheum officinale, 9 parts of Divine Comedy, 6 parts of dried orange peel, 5 parts of hawthorn, 6 parts of Radix Glycyrrhizae, 8 parts of Prunella vulgaris, Herba Cistanches 7 parts, 6 parts of Radix Astragali, 5 parts of Radix Codonopsis, 6 parts of pimiento and 7 parts of radix bupleuri pulverize in micronizer, cross 40 meshes, are mixed Fine powder is closed, low temperature drying saves, spare;
(2) 7 parts and 5 parts of sulphur of medical stone are smashed, levigation respectively, crosses 50 meshes, obtains medical stone fine powder and sulphur is thin Powder, low temperature drying saves, spare;
(3) by medical stone fine powder and sulphur fine powder made from mixing fine powders made from (1) step, step (2) and compound Vitamin (mass ratio of Cortex Phellodendri is 10:8 in multi-vitamins and step (1)) mixes, the ultraviolet sterilization in aseptic operating platform 30min is improved the immunopotentiator of soft-shelled turtle premunition.
Comparative example 2
(1) by 8 parts of the component Cortex Phellodendri of immunopotentiating composition, 9 parts of Divine Comedy, 6 parts of dried orange peel, 5 parts of hawthorn, 7 parts of Herba Cistanches, Huang 6 parts of stilbene, 5 parts of Radix Codonopsis and 6 parts of pimiento pulverize in micronizer, cross 40 meshes, obtain mixing fine powders, low temperature drying It saves, it is spare;
(2) 7 parts and 5 parts of sulphur of the component medical stone of immunopotentiating composition are smashed, levigation respectively, crosses 50 meshes, obtains To medical stone fine powder and sulphur fine powder, low temperature drying is saved, spare;
(3) medical stone fine powder made from mixing fine powders made from (1) step, step (2) and sulphur fine powder are mixed, in nothing Ultraviolet sterilization 30min in bacterium station, obtains immunopotentiating composition, spare;
(4) by lactobacillus acidophilus powder, bacillus subtilis powder, saccharomycete powder and photosynthetic bacteria powder according to mass ratio 3:3:2: 2 mixing, obtain zymophyte powder, zymophyte powder are inoculated in LB culture medium according to the ratio of 8wt%, 37 DEG C of culture 48h are obtained Primary seed solution;Primary seed solution is inoculated in LB culture medium according to the ratio of 8wt%, 37 DEG C of culture 40h obtain second level kind Sub- liquid;
(5) Immune-enhancing effect made from step (3) is added according to mass ratio 1:1.1 in secondary seed solution made from step (4) In composition, 38 DEG C of fermentation 48h be dry, pulverize, then with the multi-vitamins (matter of Cortex Phellodendri in multi-vitamins and step (1) Amount is than being 10:8) mixing, it is improved the immunopotentiator of soft-shelled turtle premunition;
(6) by the obtained immunopotentiator for improving soft-shelled turtle premunition of step (5) further with enzyme process maltodextrin according to Mass ratio 4:1 mixing, is made the immunopotentiator particle of the raising soft-shelled turtle premunition of 15 mesh, and low temperature drying saves.
Effect example
In Xinyang breeding soft-shell turtle base to raising soft-shelled turtle premunition made from embodiment 1 and comparative example 1~2 The effect of immunopotentiator particle compares test:
1000 young soft-shelled turtles are selected, are divided into five groups, the identical soft-shelled turtle feed of five groups of feedings, group 1~3 is raised in the soft-shelled turtle of feeding Embodiment 1 is added in material and the immunopotentiator particle obtained for improving soft-shelled turtle premunition of comparative example 1~2, group 4 are being raised Common soft-shelled turtle feed additive (commercially available) is added in the soft-shelled turtle feed fed, group 5 does not add any feeding in the soft-shelled turtle feed of feeding Feed additives.In breeding process, for immunopotentiator from young soft-shelled turtle at during soft-shelled turtle, the additive amount of immunopotentiator or additive is first The 2% of fish meal total weight;At the soft-shelled turtle phase and after, the additive amount of immunopotentiator or additive is soft-shelled turtle feed total weight 5%.Breeding cycle is 270 days, and feeding environment, yard feeding pattern and feeding quality are strictly consistent.Statistics cultivation in 270 days The average initial weight of soft-shelled turtle, average weight, survival rate and feed coefficient eventually in journey.
Through counting, the results are shown in Table 1.
The effect assessment of the immunopotentiator particle of the high soft-shelled turtle premunition of table 1
From the above data, for the average weight eventually and average weight gain for organizing 1 soft-shelled turtle compared with blank group height, average survival is obvious It improves.Compared with the control group without fermented immunopotentiator (group 2) obtained, survival rate can be improved to a certain extent, but It is that feed coefficient is but not much different with blank group.Lack rheum officinale, Radix Glycyrrhizae, Prunella vulgaris and radix bupleuri component, it is fermented obtained immune Although the average weight eventually of reinforcing agent and average weight gain are compared with blank group height, survival rate is lower than group 1.
In addition, the immunopotentiator particle produced by the present invention for improving soft-shelled turtle premunition contains plant polyose, Huang through detecting The Organic nutrients such as ketone, albumen, alkaloid, saponin, tannic acid matter, have stomach invigorating and digestion promoting effects simultaneously, and tonifying middle-Jiao and Qi is clearing heat and detoxicating, Anti-inflammatory analgesic also has broad-spectrum antibacterial action, effectively improves the digestion and absorption efficiency of effective component of chinese medicine, promote the growth of soft-shelled turtle The culture-cycle is shortened in development, improves culture efficiency.
The immunopotentiator particle produced by the present invention for improving soft-shelled turtle premunition also can be applied to other aquatic livestocks Cultivation.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention, It should be equivalent substitute mode, be included within the scope of the present invention.

Claims (10)

1. a kind of immunopotentiator for improving soft-shelled turtle premunition, it is characterised in that fermented by immunopotentiating composition and zymophyte powder It is prepared;
The immunopotentiating composition includes component as follows in parts by mass:
6~10 parts of Cortex Phellodendri, 4~6 parts of rheum officinale, 5~10 parts of Divine Comedy, 4~7 parts of dried orange peel, 3~8 parts of hawthorn, 6~7 parts of Radix Glycyrrhizae, summer are withered 7~9 parts of grass, 6~8 parts of Herba Cistanches, 5~7 parts of Radix Astragali, 5~7 parts of Radix Codonopsis, 5~8 parts of pimiento, 6~8 parts of radix bupleuri, medical stone 6~ 8 parts, 4~6 parts of sulphur;
The zymophyte powder includes lactobacillus acidophilus powder, bacillus subtilis powder, saccharomycete powder and photosynthetic bacteria powder.
2. the immunopotentiator according to claim 1 for improving soft-shelled turtle premunition, it is characterised in that:
The lactobacillus acidophilus powder, bacillus subtilis powder, saccharomycete powder and photosynthetic bacteria powder mass ratio be 3:(2~4): 2:(1~2).
3. the immunopotentiator according to claim 1 for improving soft-shelled turtle premunition, it is characterised in that:
Lactobacillus acidophilus powder, bacillus subtilis powder, saccharomycete powder and the photosynthetic bacteria powder is not less than 1 × 109cfu/g。
4. the immunopotentiator according to claim 1 for improving soft-shelled turtle premunition, it is characterised in that:
The immunopotentiator of the high soft-shelled turtle premunition also includes multi-vitamins, wherein the matter of multi-vitamins and Cortex Phellodendri Amount is than being (8~12): (6~10).
5. the preparation method of the described in any item immunopotentiators for improving soft-shelled turtle premunition of Claims 1 to 4, it is characterised in that It comprises the following steps:
(1) by the component Cortex Phellodendri of immunopotentiating composition, rheum officinale, Divine Comedy, dried orange peel, hawthorn, Radix Glycyrrhizae, Prunella vulgaris, Herba Cistanches, Huang Stilbene, Radix Codonopsis, pimiento and radix bupleuri pulverize, and sieving obtains mixing fine powders;
(2) the component medical stone and sulphur of immunopotentiating composition are smashed respectively, levigation, be sieved, obtain medical stone fine powder and Sulphur fine powder;
(3) medical stone fine powder made from mixing fine powders made from (1) step, step (2) and sulphur fine powder are mixed, is immunized Enhance composition;
(4) lactobacillus acidophilus powder, bacillus subtilis powder, saccharomycete powder and photosynthetic bacteria powder are mixed, obtains zymophyte powder, it will Zymophyte powder is inoculated in LB culture medium according to the ratio of 6~10wt%, and 36~38 DEG C of 40~50h of culture obtain first order seed Liquid;Primary seed solution is inoculated in LB culture medium according to the ratio of 6~10wt%, 36~38 DEG C of 35~45h of culture obtain two Grade seed liquor;
(5) by secondary seed solution made from step (4) according to mass ratio 1:(1~1.5) immune made from step (3) increase is added In strong composition, 37~39 DEG C of 36~72h of fermentation dry, pulverize, be improved the immunopotentiator of soft-shelled turtle premunition;If The component for improving the immunopotentiator of soft-shelled turtle premunition also includes multi-vitamins, then by multi-vitamins and smashed product It is mixed with multi-vitamins, is improved the immunopotentiator of soft-shelled turtle premunition.
6. the preparation method of the immunopotentiator according to claim 5 for improving soft-shelled turtle premunition, it is characterised in that:
The mass ratio of secondary seed solution described in step (5) and immunopotentiating composition is 1:1.1.
7. the preparation method of the immunopotentiator according to claim 5 for improving soft-shelled turtle premunition, it is characterised in that:
Fermentation condition described in step (5) is 38 DEG C of fermentation 48h.
8. the preparation method of the immunopotentiator according to claim 5 for improving soft-shelled turtle premunition, it is characterised in that:
The immunopotentiator of the raising soft-shelled turtle premunition is further mixed with enzyme process maltodextrin, is prepared and is improved soft-shelled turtle The immunopotentiator particle of premunition.
9. the preparation method of the immunopotentiator according to claim 8 for improving soft-shelled turtle premunition, it is characterised in that:
The immunopotentiator of raising soft-shelled turtle premunition and the mass ratio of enzyme process maltodextrin is 4:1.
10. described in any item immunopotentiator the answering in soft-shelled turtle raising field for improving soft-shelled turtle premunition of Claims 1 to 4 With.
CN201811141330.7A 2018-09-28 2018-09-28 A kind of immunopotentiator and the preparation method and application thereof improving soft-shelled turtle premunition Pending CN109156624A (en)

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