CN104435594A - Medicine for treating viral hemorrhagic disease of trionyx sinensis - Google Patents

Medicine for treating viral hemorrhagic disease of trionyx sinensis Download PDF

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CN104435594A
CN104435594A CN201410609561.1A CN201410609561A CN104435594A CN 104435594 A CN104435594 A CN 104435594A CN 201410609561 A CN201410609561 A CN 201410609561A CN 104435594 A CN104435594 A CN 104435594A
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parts
radix
vitamin
medicine
rhizoma
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占水秀
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    • A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
    • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
    • A61K31/355—Tocopherols, e.g. vitamin E
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Abstract

The invention discloses a medicine for treating the viral hemorrhagic disease of trionyx sinensis. The medicine comprises the following components: radix isatidis, fructus forsythia, flos lonicerae, flos chrysanthemi indici, cordate houttuynia, liquorices, andrographis paniculate, radix paeoniae rubra, blackberry lily, pine needle, sodium chloride, radix astragali, goldthread root, rheum officinale, phellodendron, radix sophorae falvescentis, garlic, rhizoma calami, fructus cnidii, dried tangerine peel, hawthorns, camphor, rhizome of cyrtomium, dandelion, herba epimedii, potassium sulphate, sodium bicarbonate, vitamin C, vitamin E and vitamin K3. The medicine for treating the viral hemorrhagic disease of trionyx sinensis disclosed by the invention restrains virus, can be used for treating the viral hemorrhagic disease of turtles with effective percent of over 95%, and the cure rate in 20 days reaches 90 percent above; and the medicine for treating the viral hemorrhagic disease of trionyx sinensis has reasonable compatibility, can resist fungi as well as remove garlic stink due to the use of dried tangerine peel, hawthorns, cordate houttuynia, flos lonicerae, flos chrysanthemi indici, phellodendron and the like; the preparation method of the medicine for treating the viral hemorrhagic disease of trionyx sinensis can improve the extraction rate of effective ingredients and reduce the loss of useful substances; and firstly mixing garlic with dried tangerine peel can remove the stink of garlic in advance.

Description

A kind of medicine for the treatment of Trionyx Sinensis Virus hemorrhage
Technical field
The present invention relates to the medicine relating to and treat Trionyx sinensis (Wiegmann) hemorrhage, it contains Chinese medicine ingredients and composition is repaired in nutrition.
Background technology
The hemorrhage of Trionyx sinensis (Wiegmann) is considered to be caused by virus." China's trionyx disease and study on prevention progress thereof " discloses the result of study of forefathers, and it is pointed out, hemorrhage is the later stage disease of blister sore to have researcher to think, controls from heavy, considers the body constitution impact of later stage Trionyx sinensis Wiegmann simultaneously.Researcher is also had to think that spherical viruses is inducement." comprehensive preventive health measures of Trionyx sinensis Wiegmann class disease " also discloses similar content.Trionyx disease is complicated, is difficult to quantitative analysis, and later stage researcher deflection is being determined under the prerequisite that virus causes, and adopt antiviral drug treatment, no matter specifically which kind of is viral, experimental verification curative effect is up to standard." widely popularize application Chinese herbal medicine to prevent and treat trionyx disease " discloses the inhibitory action of Chinese medicine to virus, but do not relate to can for the concrete compound recipe of Trionyx sinensis (Wiegmann)." China's trionyx disease and study on prevention progress thereof " describes the Common drugs for the treatment of viral hemorrhagic disease, and western medicine toxic and side effects is large, and single medicine easily makes virus develop immunity to drugs.201310386969.2 relate to a kind of medicine for the treatment of turtle cavity disease, it discloses the antiviral effect of part Chinese medicine.
Summary of the invention
The present invention proposes a kind of medicine for the treatment of Trionyx Sinensis Virus hemorrhage, and it is sick that it can treat Trionyx sinensis Wiegmann hemorrhage, Be very effective.
For solving the problems of the technologies described above, the present invention constructs following technical proposals:
A kind of medicine for the treatment of Trionyx Sinensis Virus hemorrhage, it is characterized in that, according to the mass fraction, consist of the following composition: Radix Isatidis 12 parts, Fructus Forsythiae 12 parts, Flos Lonicerae 12 parts, Flos Chrysanthemi Indici 12 parts, Herba Houttuyniae 12 parts, 10 parts, Radix Glycyrrhizae, Herba Andrographis 10 parts, Radix Paeoniae Rubra 10 parts, Rhizoma Belamcandae 10 parts, 10 parts, Folium Pini, 10 parts, sodium chloride, the Radix Astragali 9 parts, Rhizoma Coptidis 9 parts, Radix Et Rhizoma Rhei 9 parts, Cortex Phellodendri 9 parts, Radix Sophorae Flavescentis 8 parts, 8 parts, Bulbus Allii, Rhizoma Acori Graminei 8 parts, Fructus Cnidii 8 parts, Pericarpium Citri Reticulatae 6 parts, Fructus Crataegi 6 parts, Camphora 6 parts, Rhizoma Osmundae 5 parts, Herba Taraxaci 5 parts, Herba Epimedii 5 parts, potassium sulfate 3 parts, sodium bicarbonate 3 parts, vitamin C 0.2 part, vitamin E 0.1 part, vitamin K 30.1 part.
A kind of medicine for the treatment of Trionyx Sinensis Virus hemorrhage, it is characterized in that, according to the mass fraction, consist of the following composition: Radix Isatidis 15 parts, Fructus Forsythiae 15 parts, Flos Lonicerae 12 parts, Flos Chrysanthemi Indici 12 parts, Herba Houttuyniae 12 parts, 10 parts, Radix Glycyrrhizae, Herba Andrographis 10 parts, Radix Paeoniae Rubra 10 parts, Rhizoma Belamcandae 10 parts, 10 parts, Folium Pini, 10 parts, sodium chloride, the Radix Astragali 9 parts, Rhizoma Coptidis 9 parts, Radix Et Rhizoma Rhei 9 parts, Cortex Phellodendri 9 parts, Radix Sophorae Flavescentis 8 parts, 8 parts, Bulbus Allii, Rhizoma Acori Graminei 8 parts, Fructus Cnidii 8 parts, Pericarpium Citri Reticulatae 6 parts, Fructus Crataegi 6 parts, Camphora 6 parts, Rhizoma Osmundae 5 parts, Herba Taraxaci 5 parts, Herba Epimedii 5 parts, potassium sulfate 3 parts, sodium bicarbonate 3 parts, vitamin C 0.1 part, vitamin E 0.1 part, vitamin K 30.1 part.
A kind of medicine for the treatment of Trionyx Sinensis Virus hemorrhage, it is characterized in that, according to the mass fraction, consist of the following composition: Radix Isatidis 12 parts, Herba Andrographis 12 parts, Fructus Forsythiae 12 parts, Flos Lonicerae 12 parts, Flos Chrysanthemi Indici 12 parts, Herba Houttuyniae 12 parts, 10 parts, Radix Glycyrrhizae, Radix Paeoniae Rubra 10 parts, Rhizoma Belamcandae 10 parts, 10 parts, Folium Pini, 10 parts, sodium chloride, the Radix Astragali 9 parts, Rhizoma Coptidis 9 parts, Radix Et Rhizoma Rhei 9 parts, Cortex Phellodendri 9 parts, Radix Sophorae Flavescentis 8 parts, 8 parts, Bulbus Allii, Rhizoma Acori Graminei 8 parts, Fructus Cnidii 8 parts, Fructus Crataegi 8 parts, Camphora 5 parts, Pericarpium Citri Reticulatae 5 parts, Rhizoma Osmundae 5 parts, Herba Taraxaci 5 parts, Herba Epimedii 5 parts, sodium bicarbonate 3 parts, potassium sulfate 1 part, vitamin C 0.2 part, vitamin E 0.1 part, vitamin K 30.1 part.
Treat a preparation method for the medicine of Trionyx Sinensis Virus hemorrhage, it is characterized in that comprising the following steps:
Pulverized by the sodium chloride of Pericarpium Citri Reticulatae, Fructus Crataegi and half, add the water of 2 times of quality, 2h at a simmer, extracting juice, Bulbus Allii is pulverized, and adds in medicine juice, obtains Bulbus Allii mixed liquor;
By Herba Houttuyniae, Flos Lonicerae, Flos Chrysanthemi Indici mixing, in 80 DEG C of water-baths, be the alcoholic solution reflux, extract, filtrate 2 times of 70% by the volume fraction of 10 times amount, each 2h, merging extracted twice liquid;
Other Chinese medicinal components are mixed, adds the water of twice quality, be warming up to 60 DEG C, multistage microwave amplifier under power 400W, frequency 2450MHZ, extraction time 30min, filter, obtain filtrate;
Mixing filtrate, Bulbus Allii mixed liquor and extracting solution, decoct, until yield rate is 1g/ml, adds the water of vitamin C, vitamin E, vitamin K3, residue sodium chloride, sodium bicarbonate, potassium sulfate and 4 times of quality, namely make described medicine.
Drug on viral of the present invention has restraint, and may be used for treatment Trionyx sinensis Wiegmann viral hemorrhagic disease, effective percentage also reaches more than 90 percent more than the cure rate in 95%, 20d.Reasonable compatibility, Pericarpium Citri Reticulatae, Fructus Crataegi, Herba Houttuyniae, Flos Lonicerae, Flos Chrysanthemi Indici, Cortex Phellodendri etc. eliminate the heavy taste of Bulbus Allii at antifungal simultaneously.Preparation method can improve effective component extraction rate, and reduces the loss of benefit materials.Bulbus Allii first mixes with Pericarpium Citri Reticulatae etc., de-tastes in advance.
Detailed description of the invention
Below provide some embodiments, and record its curative effect, the item do not described in detail in embodiment is see the common process in Chemistry for Chinese Traditional Medicine field, and detailed pharmacological action can see " Chinese Pharmacopoeia (2010 editions) ".
Embodiment one
The medicine for the treatment of Trionyx Sinensis Virus hemorrhage, according to the mass fraction, consist of the following composition: Radix Isatidis 12 parts, Fructus Forsythiae 12 parts, Flos Lonicerae 12 parts, Flos Chrysanthemi Indici 12 parts, Herba Houttuyniae 12 parts, 10 parts, Radix Glycyrrhizae, Herba Andrographis 10 parts, Radix Paeoniae Rubra 10 parts, Rhizoma Belamcandae 10 parts, 10 parts, Folium Pini, 10 parts, sodium chloride, the Radix Astragali 9 parts, Rhizoma Coptidis 9 parts, Radix Et Rhizoma Rhei 9 parts, Cortex Phellodendri 9 parts, Radix Sophorae Flavescentis 8 parts, 8 parts, Bulbus Allii, Rhizoma Acori Graminei 8 parts, Fructus Cnidii 8 parts, Pericarpium Citri Reticulatae 6 parts, Fructus Crataegi 6 parts, Camphora 6 parts, Rhizoma Osmundae 5 parts, Herba Taraxaci 5 parts, Herba Epimedii 5 parts, potassium sulfate 3 parts, sodium bicarbonate 3 parts, vitamin C 0.2 part, vitamin E 0.1 part, vitamin K 30.1 part.
Embodiment two
The medicine for the treatment of Trionyx Sinensis Virus hemorrhage, according to the mass fraction, consist of the following composition: Radix Isatidis 12 parts, Herba Andrographis 12 parts, Fructus Forsythiae 12 parts, Flos Lonicerae 12 parts, Flos Chrysanthemi Indici 12 parts, Herba Houttuyniae 12 parts, 10 parts, Radix Glycyrrhizae, Radix Paeoniae Rubra 10 parts, Rhizoma Belamcandae 10 parts, 10 parts, Folium Pini, 10 parts, sodium chloride, the Radix Astragali 9 parts, Rhizoma Coptidis 9 parts, Radix Et Rhizoma Rhei 9 parts, Cortex Phellodendri 9 parts, Radix Sophorae Flavescentis 8 parts, 8 parts, Bulbus Allii, Rhizoma Acori Graminei 8 parts, Fructus Cnidii 8 parts, Fructus Crataegi 8 parts, Camphora 5 parts, Pericarpium Citri Reticulatae 5 parts, Rhizoma Osmundae 5 parts, Herba Taraxaci 5 parts, Herba Epimedii 5 parts, sodium bicarbonate 3 parts, potassium sulfate 1 part, vitamin C 0.2 part, vitamin E 0.1 part, vitamin K 30.1 part.
Embodiment three
The medicine for the treatment of Trionyx Sinensis Virus hemorrhage, according to the mass fraction, consist of the following composition: Radix Isatidis 15 parts, Fructus Forsythiae 15 parts, Flos Lonicerae 12 parts, Flos Chrysanthemi Indici 12 parts, Herba Houttuyniae 12 parts, 10 parts, Radix Glycyrrhizae, Herba Andrographis 10 parts, Radix Paeoniae Rubra 10 parts, Rhizoma Belamcandae 10 parts, 10 parts, Folium Pini, 10 parts, sodium chloride, the Radix Astragali 9 parts, Rhizoma Coptidis 9 parts, Radix Et Rhizoma Rhei 9 parts, Cortex Phellodendri 9 parts, Radix Sophorae Flavescentis 8 parts, 8 parts, Bulbus Allii, Rhizoma Acori Graminei 8 parts, Fructus Cnidii 8 parts, Pericarpium Citri Reticulatae 6 parts, Fructus Crataegi 6 parts, Camphora 6 parts, Rhizoma Osmundae 5 parts, Herba Taraxaci 5 parts, Herba Epimedii 5 parts, potassium sulfate 3 parts, sodium bicarbonate 3 parts, vitamin C 0.1 part, vitamin E 0.1 part, vitamin K 30.1 part.
Preparation method comprises the following steps:
Pulverized by the sodium chloride of Pericarpium Citri Reticulatae, Fructus Crataegi and half, add the water of 2 times of quality, 2h at a simmer, extracting juice, Bulbus Allii is pulverized, and adds in medicine juice, obtains Bulbus Allii mixed liquor; By Herba Houttuyniae, Flos Lonicerae, Flos Chrysanthemi Indici mixing, in 80 DEG C of water-baths, be the alcoholic solution reflux, extract, filtrate 2 times of 70% by the volume fraction of 10 times amount, each 2h, merging extracted twice liquid; Other Chinese medicinal components are mixed, adds the water of twice quality, be warming up to 60 DEG C, multistage microwave amplifier under power 400W, frequency 2450MHZ, extraction time 30min, filter, obtain filtrate; Mixing filtrate, Bulbus Allii mixed liquor and extracting solution, decoct, until yield rate is 1g/ml, adds the water of vitamin C, vitamin E, vitamin K3, residue sodium chloride, sodium bicarbonate, potassium sulfate and 4 times of quality, namely make described medicine.
Experiment one
Test method:
1, plant is divided into the test tank of equal-volume (50cm × 50cm × 40cm), is labeled as 30 groups according to Chinese medicine ingredients.Often group adopts same water source (tap water after lake water or aeration), without being communicated with between group.Water temperature 20 to 28 DEG C, pH=6-8.
2, get Trionyx sinensis Wiegmann 600 tail at random in ill plant, be placed in test tank by 20 tails/component.Every day throws in bait by the 3-4% of Trionyx sinensis Wiegmann body weight, and 8: 00,13: 00,16: 00 respectively throw something and feed once.Every day puts half water from bottom, injects water source to 60 ± 10cm simultaneously.
3, daily check, takes out dead or is about to dead Trionyx sinensis Wiegmann, in order to avoid cross infection, affecting result of the test.
Experimental Comparison:
Different Chinese herbal medicine decoctions is thrown according to group name.According to reality survival number, delimit the effect of the monomer in treating skin fester disease of each Chinese medicine ingredients as following table: it is better that "+" represents curative effect more.
Through test, the present invention chooses some drugs, simultaneously proportion optimizing.Bulbus Allii plays therapeutic effect, and Flos Chrysanthemi Indici, Flos Lonicerae, Fructus Crataegi, Pericarpium Citri Reticulatae etc. de-taste.
Test two
Test material and environment: test Trionyx sinensis Wiegmann derives from breeding soft-shell turtle field, Nanchang district, Jiangxi Province, and the gross area is about 980m 2, total Trionyx sinensis Wiegmann number about 30000,2012, there is fish molds in certain culture zone of this plant, Trionyx sinensis Wiegmann is sporadicly dead.Trionyx sinensis Wiegmann test site is located at Duchang County, Jiangxi Province, is less than 10km apart from poyang lake water, and environment is suitable for Trionyx sinensis Wiegmann existence.Between test period April to June, cultivation 30d.
Test method:
1, plant is divided into five groups of test tanks of equal-volume (300cm × 300cm × 80cm), is labeled as A experimental group, B experimental group, C experimental group, A matched group, B matched group respectively.Often group adopts same water source (tap water after lake water or aeration), without being communicated with between group.Water temperature 20 to 28 DEG C, pH=6-8.
2, get test Trionyx sinensis Wiegmann 500 tail, be placed in five groups of test tanks by 100 tails/component.Every day throws in fish material by the 3-4% of Trionyx sinensis Wiegmann body weight, and 8: 00,13: 00,16: 00 respectively throw something and feed once.Every day puts half water from bottom, injects water source to 60 ± 10cm simultaneously.
3, daily check, finds (sensory evaluation, the statistical significance) of disease symptoms, takes out or isolation, in order to avoid cross infection, affects result of the test.
Experimental Comparison:
1, build cm × 80cm medicine pond, five group of 300 cm × 300, be labeled as A experimental group, B experimental group, C experimental group, A matched group, B matched group (matched group separate case compares) respectively.
2, medicine of the present invention is injected respectively the medicine pond of A experimental group, B experimental group, C experimental group, the degree of depth 30 ± 5 cm.Sodium bicarbonate water, saline solution inject the medicine pond of A matched group, and B matched group only injects water (or the tap water after lake water or aeration).
3, every 3 days by Trionyx sinensis Wiegmann take out, correspondence put into medicine pond soak 10 to 20 minutes, each medicine pond single only puts into a tail, after Orthogonal Rotational Regressive Tests pond.
Along with the passing of culturing time, in test tank, dead Trionyx sinensis Wiegmann mantissa is as following table:
Soda water, the Sal prevention and control ability to virus is little.The later death quantity of experimental group is few, and the state of an illness is controlled, and curative effect of medication of the present invention is remarkable.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (5)

1. treat a medicine for Trionyx Sinensis Virus hemorrhage, it is characterized in that, comprise Radix Isatidis, Flos Lonicerae, Fructus Forsythiae and Flos Chrysanthemi Indici.
2. medicine according to claim 1, it is characterized in that, according to the mass fraction, consist of the following composition: Radix Isatidis 12 parts, Fructus Forsythiae 12 parts, Flos Lonicerae 12 parts, Flos Chrysanthemi Indici 12 parts, Herba Houttuyniae 12 parts, 10 parts, Radix Glycyrrhizae, Herba Andrographis 10 parts, Radix Paeoniae Rubra 10 parts, Rhizoma Belamcandae 10 parts, 10 parts, Folium Pini, 10 parts, sodium chloride, the Radix Astragali 9 parts, Rhizoma Coptidis 9 parts, Radix Et Rhizoma Rhei 9 parts, Cortex Phellodendri 9 parts, Radix Sophorae Flavescentis 8 parts, 8 parts, Bulbus Allii, Rhizoma Acori Graminei 8 parts, Fructus Cnidii 8 parts, Pericarpium Citri Reticulatae 6 parts, Fructus Crataegi 6 parts, Camphora 6 parts, Rhizoma Osmundae 5 parts, Herba Taraxaci 5 parts, Herba Epimedii 5 parts, potassium sulfate 3 parts, sodium bicarbonate 3 parts, vitamin C 0.2 part, vitamin E 0.1 part, vitamin K 30.1 part.
3. medicine according to claim 1, it is characterized in that, according to the mass fraction, consist of the following composition: Radix Isatidis 15 parts, Fructus Forsythiae 15 parts, Flos Lonicerae 12 parts, Flos Chrysanthemi Indici 12 parts, Herba Houttuyniae 12 parts, 10 parts, Radix Glycyrrhizae, Herba Andrographis 10 parts, Radix Paeoniae Rubra 10 parts, Rhizoma Belamcandae 10 parts, 10 parts, Folium Pini, 10 parts, sodium chloride, the Radix Astragali 9 parts, Rhizoma Coptidis 9 parts, Radix Et Rhizoma Rhei 9 parts, Cortex Phellodendri 9 parts, Radix Sophorae Flavescentis 8 parts, 8 parts, Bulbus Allii, Rhizoma Acori Graminei 8 parts, Fructus Cnidii 8 parts, Pericarpium Citri Reticulatae 6 parts, Fructus Crataegi 6 parts, Camphora 6 parts, Rhizoma Osmundae 5 parts, Herba Taraxaci 5 parts, Herba Epimedii 5 parts, potassium sulfate 3 parts, sodium bicarbonate 3 parts, vitamin C 0.1 part, vitamin E 0.1 part, vitamin K 30.1 part.
4. medicine according to claim 1, it is characterized in that, according to the mass fraction, consist of the following composition: Radix Isatidis 12 parts, Herba Andrographis 12 parts, Fructus Forsythiae 12 parts, Flos Lonicerae 12 parts, Flos Chrysanthemi Indici 12 parts, Herba Houttuyniae 12 parts, 10 parts, Radix Glycyrrhizae, Radix Paeoniae Rubra 10 parts, Rhizoma Belamcandae 10 parts, 10 parts, Folium Pini, 10 parts, sodium chloride, the Radix Astragali 9 parts, Rhizoma Coptidis 9 parts, Radix Et Rhizoma Rhei 9 parts, Cortex Phellodendri 9 parts, Radix Sophorae Flavescentis 8 parts, 8 parts, Bulbus Allii, Rhizoma Acori Graminei 8 parts, Fructus Cnidii 8 parts, Fructus Crataegi 8 parts, Camphora 5 parts, Pericarpium Citri Reticulatae 5 parts, Rhizoma Osmundae 5 parts, Herba Taraxaci 5 parts, Herba Epimedii 5 parts, sodium bicarbonate 3 parts, potassium sulfate 1 part, vitamin C 0.2 part, vitamin E 0.1 part, vitamin K 30.1 part.
5. treat a preparation method for the medicine of Trionyx Sinensis Virus hemorrhage, it is characterized in that comprising the following steps:
Pulverized by the sodium chloride of Pericarpium Citri Reticulatae, Fructus Crataegi and half, add the water of 2 times of quality, 2h at a simmer, extracting juice, Bulbus Allii is pulverized, and adds in medicine juice, obtains Bulbus Allii mixed liquor;
By Herba Houttuyniae, Flos Lonicerae, Flos Chrysanthemi Indici mixing, in 80 DEG C of water-baths, be the alcoholic solution reflux, extract, filtrate 2 times of 70% by the volume fraction of 10 times amount, each 2h, merging extracted twice liquid;
Other Chinese medicinal components are mixed, adds the water of twice quality, be warming up to 60 DEG C, multistage microwave amplifier under power 400W, frequency 2450MHZ, extraction time 30min, filter, obtain filtrate;
Mixing filtrate, Bulbus Allii mixed liquor and extracting solution, decoct, until yield rate is 1g/ml, adds the water of vitamin C, vitamin E, vitamin K3, residue sodium chloride, sodium bicarbonate, potassium sulfate and 4 times of quality, namely make described medicine.
CN201410609561.1A 2014-11-04 2014-11-04 Medicine for treating viral hemorrhagic disease of trionyx sinensis Pending CN104435594A (en)

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